CN107027662A - A kind of bar house oil fish hybridization triploid artificial propagation techniques - Google Patents
A kind of bar house oil fish hybridization triploid artificial propagation techniques Download PDFInfo
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- CN107027662A CN107027662A CN201710180290.6A CN201710180290A CN107027662A CN 107027662 A CN107027662 A CN 107027662A CN 201710180290 A CN201710180290 A CN 201710180290A CN 107027662 A CN107027662 A CN 107027662A
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- house oil
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- parent population
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
It is contemplated that a kind of bar house oil fish artificial fecundation method is disclosed, using following steps：Set up fishpond, select parent population, artificial insemination, artificial incubation, cultivation seed before parent population, injection ocyodinic, management spawning; it is slow that the present invention solves bar house oil fish gonad development; female, the nonsynchronous method of milter gonad development; and propose fry opening material and instruct feeding for feed, seedling can be provided for large-scale cultivation bar house oil fish.
Description
Technical field
The present invention relates to belong to cultural technique field, more particularly to a kind of bar house oil fish hybridization triploid artificial propagation skill
Art.
Background technology
Bar house oil fish original name bar horse Sui lips Barb（Crossocheilus bamaensis Fang,sp.nov.）, bar horse intend tassel
Fish（Pseudocrossocheilus bamaensis Fang）, main product in the Pan Yanghe in Guangxi China bar horse border of the county, and
Hongsuihe River mainstream, is the rare small-sized economic fish of Guangxi rivers original inhabitants.Its delicious meat, edibility are high and with one
Fixed medical value, is described as " ginseng under water ", is one of township Bama County of Guangxi " bar horse Triratna " of Chinese Famous longevity.
The requirement that bar house oil fish is perched, survived, grown and bred to environmental condition is special, propagates middle gonad development artificially
Slowly, female, milter gonad development is asynchronous, and scale artificial culture can not be carried out so far.Current bar house oil fish eats fish market and sold
Valency has price but no buyers in 200 yuan/more than kg（Fish）.The vigorous market demand, urgent need is carried out its large-scale artificial and bred, and passes through
The popularization cultivation of artificial breeding seed, alleviates its market supply and demand contradiction.Since nineteen eighty-two, multiple departments successively carry out in Guangxi District
The Primary Study experiment to bar house oil fish related, but still fail to break through its large-scale artificial breeding key technology, seafood so far
In the market bar house oil fish culture product is still in blank stage.Plus manually excessively capturing for many years, stealing and catch, rivers fish germ plasm resource connects
It is near exhausted.Carry out bar house oil fish artificial domesticating and cultivating and biological control technical research, artificial breeding nursery is supplied to social batch
Kind, help to drive the development of bar house oil fish culture industry and Poor Mountainous Area peasant cultivation to get rich, if meeting national at present and following
The estate planning in dry year and supply side reform orientation；To realizing bar house oil fish enhancement releasing, protection River Fish resource, promoting bar
The industrialization of house oil fish and the development of local tour tourism business have important meaning view.
Bar house oil fish is slow-growing, and population quantity is drastically reduced, and large-scale farming is provided without seedling, the Poor Mountainous Area people
The masses shake off poverty and set out on the road to prosperity in the urgent need to a kind of improved seeds of energy large-scale farming, add bar house oil fish population deterioration seriously early,
It is badly in need of breed improvement, the cultivation amount of bar house oil fish is improved comprehensively, polyploid breeding is to solve improvement population quality and scale breeding
Key means and method, because the key of triploid nursery is to find out suitable polyploid seedling, pass through genetic recombination, training
The excellent Polyploid fish of character is brought out, triploid body fish has fast growth individual greatly, and meat is good, and infertility, anti-antidromicity is strong,
The characteristics of survival rate is high, artificial induction's triploid Breeding technology is a kind of high-tech means for starting the sixties in last century to rise,
It is divided into artificial induction's thelykaryon（Arrhenokaryon）Develop diploid induction；Artificial induction's fish body triploid gene, diploid is miscellaneous with tetraploid
The three kinds of methods that obtain are handed over, it is most simple and most directly effective method that wherein Cross between diploid and tetraploid, which obtains triploid fish,
The suitable tetraploid fish of selection turns into the core of breed triploid fish, according to the chromosome ratio to mutually equal class and subfamily class fingerling
Choosing, selects close section and subfamily fish polyploid chromosome fish as hybridization parent population, using distant hybridization technology, so as to cultivate
Go out triploid hydrid bar house oil fish.
The content of the invention
In view of the above-mentioned problems, the present invention provides a kind of bar house oil fish hybridization triploid artificial propagation techniques, can be to society
Seed is provided, promotes pond, reservoir and propagates artificially, because the sterility of triploid fish does not destroy germ plasm resource, fisherman again
It can be improved the people's livelihood to accelerating fishery innovation, promote rural economy flourishing, can not only make poor while obtaining economic benefit
The tired mountain area common people obtain the improved seeds of energy large-scale farming, shake off poverty and set out on the road to prosperity early, can also realize large-scale cultivation, lift bar
The quality of house oil fish, technical support is provided for the improvement species of bar house oil fish.
To solve technical problem, the technical solution adopted by the present invention：A kind of bar house oil fish hybridization triploid artificial propagation skill
Art, comprises the following steps：
（1）Build in fishpond
Selection builds fishpond in Bama County of Guangxi or the positive Ha Noi environment of periphery mimic panel, and carries out the disinfection in fishpond, finally
The positive river of disk or the mountain spring water and underground river of cleaning are put into, and keeps certain mobility, stimulation by running water parent population is used.
（2）The selection of parent population
Annual 4-6 months and 9-12 months and select physique healthy and strong, it is disease-free without wound, the good bar house oil fish public affairs fish parent fish of gonad development with
Barbinae tetraploid parent population raun carries out artificial induced spawning；Substantially, hand is touched with the white pearl star of sexually matured bar house oil fish milter rhynchodaenm
Obvious furcella, light pressure belly is the seminal fluid outflow for having white thick；Barbinae tetraploid parent population raun belly expands, accidental ovum
Nest profile, cloacal aperture is micro- red, touches belly, and feel has certain full, pliability.
The ripe Spinibarbus sinensis of selection, hangnail Barb and black ridge hangnail Barb are used as Barbinae tetraploid parent population and bar house oil
Fish is hybridized.
（3）Inject ocyodinic
The female parent population of Barbinae tetraploid is injected using a pin, and injection site is thoracic cavity；Bar house oil fish milter is injected using a pin, note
Position is penetrated for thoracic cavity, lucifuge sunshade during injection；
The female parent population ocyodinic selection maleic acid DOM of Barbinae tetraploid is discharged by per kilogram of body weight 3-5mg and luteotropin
Hormone A2 carries out intramuscular injection by per kilogram of body weight 9-18 μ g and HCG per kilograms 1000-1500IU, and bar house oil fish milter regards maturation
Situation suitably reduces dosage or not injected；
Water temperature control is at 18~25 DEG C during artificial induced spawning, and injection implements artificial aiding-birth after 24 hours.
（4）The management of parent population before spawning
After the injection of parent population ocyodinic, it is put into constructed fishpond, shading simultaneously keeps environment quiet.
（5）Artificial insemination
The method for carrying out dry method of artificial fertilization after the injection of parent population ocyodinic for 24 hours carries out artificial insemination；Embryonated egg using unsticking or
Manually fish nest collects embryonated egg.
（6）Artificial incubation
Embryonated egg that artificial insemination is obtained is cleaned, go the removal of impurity, after mould proof sterilization, is put into hatchery and is incubated with shade net sunshade
Change；Miniflow water is kept, hatching coolant-temperature gage keeps 18-25C °, more than water body dissolved oxygen amount 5mg/L.
（7）Seed rearing
3-5 days waist points occur after fry membrane, the flat trip of fry, feed dedicated openings bait, after 14 days, are progressively transitioned into and feed specially
Perfect compound feed powder is used, when body length reaches more than 3cm, instruction raises artificial mixed feed of mediating and sinks to the bottom material.
Further：The environment in the fishpond oneself can simulate manufacture, it is desirable to：Water colour, transparency, water temperature, dissolved oxygen, pH value
Content is approached with the positive river of Bama County of Guangxi disk.
Further：The open-mouthed bait is yolk or yolk, soya-bean milk mixing or contained using the commercial protein for being crushed to 400 mesh
The compound of the eel feed powder material of amount 43% or more material.
Further：The ocyodinic selects luteinizing hormone-releasing hormone analog LRH-A2, maleic acid DOM DOM
And HCG.
Further：The Barbinae tetraploid parent population raun is injected using a pin, injects luteinizing hormone-releasing hormone
Analog LRH-A2+ maleic acid DOMs DOM and HCG, dosage 9-18ugLRH-A2+3-5mgDOM/kg+1000-1500IU/
kg.Bar house oil fish milter is injected using a pin, and injection site is thoracic cavity, and bar house oil fish milter bar house oil fish milter regards mature condition
It is appropriate to reduce dosage or do not inject.
Further：After the parent population ocyodinic injection, it is put into constructed fishpond, shading simultaneously keeps environment quiet, half
With stimulation by running water 1-2 hour after hour, afterwards interval 3 hours with stimulation by running water once.
Further：The dry method of artificial fertilization comprises the following steps：The dry method of artificial fertilization comprises the following steps：First
The fish-egg of raun is squeezed into the vessel dried, then squeezes into milter seminal fluid, is stirred with chicken, drake feather, then adds 5% grape
Syrup is stirred evenly, static 1-2 minutes, is added clear water and is stirred evenly, static 1-2 minutes.Unsticking uses 10% 400 mesh talcum powder water, people
Work stirring unsticking 30 minutes, obtains unsticking ovum.
Further：Hatching water temperature control in the fishpond is at 22-25 DEG C, using Lotic hatching, water body dissolved oxygen amount 5mg/L with
On..
Further：The ripe Spinibarbus sinensis of the Barbinae tetraploid parent population selection, hangnail Barb and black ridge hangnail Barb
It is used as hybridization triploid parent population.
Further：The Barbinae tetraploid parent population can be produced with bar house oil fish with reciprocal cross.
Beneficial effects of the present invention：In view of the above-mentioned problems, the present invention provides a kind of bar house oil fish hybridization triploid artificial breeding
Plantation technology, can provide seed to society, promote the Chi Ba pools, reservoir and propagate artificially, due to triploid fish sterility again
Germ plasm resource is not destroyed, can be improved the people's livelihood while fisherman obtains economic benefit to accelerating fishery innovation, promote rural area warp
Ji is flourishing, the Poor Mountainous Area common people is obtained the improved seeds of energy large-scale farming, shake off poverty and set out on the road to prosperity early, can also be real
Existing large-scale cultivation, lifts the quality of bar house oil fish, and technical support is provided for the improvement species of bar house oil fish.
Embodiment
The present invention will be further described for implementation process below, to facilitate the technical staff to understand.
Embodiment 1:Bar house oil fish hybridization triploid artificial propagation techniques, comprise the following steps：
（1）Build in fishpond
Selection builds fishpond in Bama County of Guangxi or the positive Ha Noi environment of periphery mimic panel, and carries out the disinfection in fishpond, finally
The positive river of disk or the mountain spring water and underground river of cleaning are put into, and keeps certain mobility, stimulation by running water parent population is used.
（2）The selection of parent population
Annual 4-6 months and 9-12 months and select physique healthy and strong, it is disease-free without wound, the good bar house oil fish public affairs fish parent fish of gonad development with
Barbinae tetraploid parent population raun carries out artificial induced spawning；Substantially, hand is touched with the white pearl star of sexually matured bar house oil fish milter rhynchodaenm
Obvious furcella, light pressure belly is the seminal fluid outflow for having white thick；Barbinae tetraploid parent population raun belly expands, accidental ovum
Nest profile, cloacal aperture is micro- red, touches belly, and feel has certain full, pliability.
The ripe Spinibarbus sinensis of selection, hangnail Barb and black ridge hangnail Barb are used as Barbinae tetraploid parent population and bar house oil
Fish is hybridized.
（3）Inject ocyodinic
The female parent population of Barbinae tetraploid is injected using a pin, and injection site is thoracic cavity；Bar house oil fish milter is injected using a pin, note
Position is penetrated for thoracic cavity, lucifuge sunshade during injection；
The female parent population ocyodinic selection maleic acid DOM of Barbinae tetraploid is swashed by per kilogram of body weight 3mg and luteotropin release
Plain A2 carries out intramuscular injection by the μ g of per kilogram of body weight 12 and per kilogram 1200IU, and bar house oil fish milter is suitably reduced depending on mature condition
Dosage is not injected；
Water temperature control is at 22 DEG C during artificial induced spawning, and injection implements artificial aiding-birth after 24 hours.
（4）The management of parent population before spawning
After the injection of parent population ocyodinic, it is put into constructed fishpond, shading simultaneously keeps environment quiet.
（5）Artificial insemination
The method for carrying out dry method of artificial fertilization after the injection of parent population ocyodinic for 24 hours carries out artificial insemination；Embryonated egg using unsticking or
Manually fish nest collects embryonated egg.
（6）Artificial incubation
Embryonated egg that artificial insemination is obtained is cleaned, go the removal of impurity, after mould proof sterilization, is put into hatchery and is incubated with shade net sunshade
Change；Miniflow water is kept, hatching coolant-temperature gage is kept for 22C °；It is oxygenated with oxygen pump to water body, it is desirable to more than water body dissolved oxygen amount 5mg/L.
（7）Seed rearing
3-5 days waist points occur after fry membrane, the flat trip of fry, feed dedicated openings bait, after 14 days, are progressively transitioned into and feed specially
Perfect compound feed powder is used, when body length reaches more than 3cm, instruction raises artificial mixed feed of mediating and sinks to the bottom material.
Above-described is only presently preferred embodiments of the present invention, all timess made in the range of the spirit and principles in the present invention
What modifications, equivalent substitutions and improvements etc., should be included in the scope of the protection.
Claims (10)
1. a kind of bar house oil fish hybridization triploid artificial propagation techniques, it is characterised in that comprise the following steps：
（1）Build in fishpond
Selection builds fishpond in Bama County of Guangxi or the positive Ha Noi environment of periphery mimic panel, and carries out the disinfection in fishpond, finally
The positive river of disk or the mountain spring water and underground river of cleaning are put into, and keeps certain mobility, stimulation by running water parent population is used；
（2）The selection of parent population
Annual 4-6 months and 9-12 months and select physique healthy and strong, it is disease-free without wound, the good bar house oil fish public affairs fish parent fish of gonad development with
Barbinae tetraploid parent population raun carries out artificial induced spawning；Substantially, hand is touched with the white pearl star of sexually matured bar house oil fish milter rhynchodaenm
Obvious furcella, light pressure belly is the seminal fluid outflow for having white thick；Barbinae tetraploid parent population raun belly expands, accidental ovum
Nest profile, cloacal aperture is micro- red, touches belly, and feel has certain full, pliability, and the ripe Spinibarbus sinensis of selection falls
Thorn Barb and black ridge hangnail Barb is hybridized as Barbinae tetraploid parent population with bar house oil fish；
（3）Inject ocyodinic
The female parent population of Barbinae tetraploid is injected using a pin, and injection site is thoracic cavity；Bar house oil fish milter is injected using a pin, note
Position is penetrated for thoracic cavity, lucifuge sunshade during injection；
The female parent population ocyodinic selection maleic acid DOM of Barbinae tetraploid is discharged by per kilogram of body weight 3-5mg and luteotropin
Hormone A2 carries out intramuscular injection by per kilogram of body weight 9-18 μ g and HCG per kilograms 1000-1500IU, and bar house oil fish milter regards maturation
Situation suitably reduces dosage or not injected；
Water temperature control is at 18~25 DEG C during artificial induced spawning, and injection implements artificial aiding-birth after 24 hours；
（4）The management of parent population before spawning
After the injection of parent population ocyodinic, it is put into constructed fishpond, shading simultaneously keeps environment quiet；
（5）Artificial insemination
The method for carrying out dry method of artificial fertilization after the injection of parent population ocyodinic for 24 hours carries out artificial insemination；Embryonated egg using unsticking or
Manually fish nest collects embryonated egg；
（6）Artificial incubation
Embryonated egg that artificial insemination is obtained is cleaned, go the removal of impurity, after mould proof sterilization, is put into hatchery and is incubated with shade net sunshade
Change；Miniflow water is kept, hatching coolant-temperature gage keeps 18-25C °, more than water body dissolved oxygen amount 5mg/L；
（7）Seed rearing
3-5 days waist points occur after fry membrane, the flat trip of fry, feed dedicated openings bait, after 14 days, are progressively transitioned into and feed specially
Perfect compound feed powder is used, when body length reaches more than 3cm, instruction raises artificial mixed feed of mediating and sinks to the bottom material.
2. a kind of bar house oil fish hybridization triploid artificial propagation techniques according to claim 1, it is characterised in that the fish
The environment in pond oneself can simulate manufacture, it is desirable to：Water colour, transparency, water temperature, dissolved oxygen, pH value content and Bama County of Guangxi disk sun
River is approached.
3. a kind of bar house oil fish hybridization triploid artificial propagation techniques according to claim 1, it is characterised in that described to open
Mouthful bait be yolk or yolk, soya-bean milk mixing or the eel feed powder material for using the commercial protein content 43% for being crushed to 400 mesh or with
The compound of upper material.
4. a kind of bar house oil fish hybridization triploid artificial propagation techniques according to claim 1, it is characterised in that described to urge
Produce agent and select luteinizing hormone-releasing hormone analog LRH-A2, maleic acid DOM DOM and HCG.
5. a kind of bar house oil fish hybridization triploid artificial propagation techniques according to claim 1, it is characterised in that the Barb
Subfamily tetraploid parent population raun is injected using a pin, injection luteinizing hormone-releasing hormone analog LRH-A2+ maleic acids
Europe ketone DOM+HCG, dosage 9-18ugLRH-A2+3-5mgDOM/kg+1000-1500IU/kg, bar house oil fish milter use a pin
Injection, injection site is thoracic cavity, and bar house oil fish milter suitably reduces dosage depending on mature condition or do not injected.
6. the bar house oil fish hybridization triploid artificial propagation techniques according to claim 1 any one, it is characterised in that institute
State after the injection of parent population ocyodinic, be put into constructed fishpond, shading simultaneously keeps environment quiet, and stimulation by running water 1- is used after half an hour
2 hours, afterwards be spaced 3 hours with stimulation by running water once.
7. bar house oil fish hybridization triploid artificial propagation techniques according to claim 1, it is characterised in that the dry method people
Work insemination comprises the following steps：The fish-egg of raun is squeezed into first in the vessel dried, then squeeze into milter seminal fluid, with chicken, drake feather
Stir, then add 5% G/W and stir evenly, it is static 1-2 minutes, add clear water and stir evenly, static 1-2 minutes, unsticking is adopted
With 10% 400 mesh talcum powder water, artificial stirring unsticking 30 minutes obtains unsticking ovum.
8. the bar house oil fish hybridization triploid artificial propagation techniques according to claim 1 any one, it is characterised in that institute
Fishpond hatching water temperature control is stated at 22-25 DEG C, using Lotic hatching, more than water body dissolved oxygen amount 5mg/L.
9. the bar house oil fish hybridization triploid artificial propagation techniques according to claim 1 any one, it is characterised in that institute
The ripe Spinibarbus sinensis of Barbinae tetraploid parent population selection is stated, hangnail Barb and black ridge hangnail Barb are used as hybridization triploid parent
Fish.
10. the bar house oil fish hybridization triploid artificial propagation techniques according to claim 1 any one, it is characterised in that
The Barbinae tetraploid parent population can produce triploid fish with bar house oil fish with reciprocal cross.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107581111A (en) * | 2017-11-08 | 2018-01-16 | 重庆市同盟农业开发有限公司 | A kind of Triploid Rainbow Trout fish seeding technique method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1263703A (en) * | 1999-02-15 | 2000-08-23 | 湖南师范大学 | Method for breeding triploid crucian carp (common carp) and apparatus thereof |
| CN101554144A (en) * | 2009-05-22 | 2009-10-14 | 中国科学院昆明动物研究所 | Artificial fertilization and incubation method of sinocyclocheilus grahami |
| CN103651220A (en) * | 2013-12-30 | 2014-03-26 | 玉溪市水产工作站（玉溪市水生动物防疫检疫站云南省渔业船舶检验局玉溪检验处） | Barbodes fuxianensis artificial propagation technology |
| CN106035235A (en) * | 2016-06-30 | 2016-10-26 | 湖南文理学院 | Efficient breeding method for silver-fish triploid |
| CN106135093A (en) * | 2016-06-30 | 2016-11-23 | 湖南文理学院 | A kind of method improving Mylopharyngodon piceus triploid incubation rate |
-
2017
- 2017-03-24 CN CN201710180290.6A patent/CN107027662A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1263703A (en) * | 1999-02-15 | 2000-08-23 | 湖南师范大学 | Method for breeding triploid crucian carp (common carp) and apparatus thereof |
| CN101554144A (en) * | 2009-05-22 | 2009-10-14 | 中国科学院昆明动物研究所 | Artificial fertilization and incubation method of sinocyclocheilus grahami |
| CN103651220A (en) * | 2013-12-30 | 2014-03-26 | 玉溪市水产工作站（玉溪市水生动物防疫检疫站云南省渔业船舶检验局玉溪检验处） | Barbodes fuxianensis artificial propagation technology |
| CN106035235A (en) * | 2016-06-30 | 2016-10-26 | 湖南文理学院 | Efficient breeding method for silver-fish triploid |
| CN106135093A (en) * | 2016-06-30 | 2016-11-23 | 湖南文理学院 | A kind of method improving Mylopharyngodon piceus triploid incubation rate |
Non-Patent Citations (1)
| Title |
|---|
| 宋灿等: "多倍体生物研究进展", 《中国科学:生命科学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107581111A (en) * | 2017-11-08 | 2018-01-16 | 重庆市同盟农业开发有限公司 | A kind of Triploid Rainbow Trout fish seeding technique method |
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