CN107014786A - 驻波干涉显微镜 - Google Patents
驻波干涉显微镜 Download PDFInfo
- Publication number
- CN107014786A CN107014786A CN201610863720.XA CN201610863720A CN107014786A CN 107014786 A CN107014786 A CN 107014786A CN 201610863720 A CN201610863720 A CN 201610863720A CN 107014786 A CN107014786 A CN 107014786A
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- 230000003287 optical effect Effects 0.000 claims abstract description 60
- 238000004458 analytical method Methods 0.000 claims abstract description 25
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- 230000001678 irradiating effect Effects 0.000 claims abstract description 5
- 238000000034 method Methods 0.000 claims description 8
- 230000001427 coherent effect Effects 0.000 claims description 6
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- 101100509369 Caenorhabditis elegans isw-1 gene Proteins 0.000 description 4
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- 238000000799 fluorescence microscopy Methods 0.000 description 2
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- 235000011464 Pachycereus pringlei Nutrition 0.000 description 1
- 240000006939 Pachycereus weberi Species 0.000 description 1
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- 238000001218 confocal laser scanning microscopy Methods 0.000 description 1
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- 230000002708 enhancing effect Effects 0.000 description 1
- 102000034287 fluorescent proteins Human genes 0.000 description 1
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/41—Refractivity; Phase-affecting properties, e.g. optical path length
- G01N21/45—Refractivity; Phase-affecting properties, e.g. optical path length using interferometric methods; using Schlieren methods
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0032—Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0052—Optical details of the image generation
- G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
- G02B21/08—Condensers
- G02B21/088—Condensers for both incident illumination and transillumination
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
- G02B21/08—Condensers
- G02B21/14—Condensers affording illumination for phase-contrast observation
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/18—Arrangements with more than one light path, e.g. for comparing two specimens
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/361—Optical details, e.g. image relay to the camera or image sensor
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/364—Projection microscopes
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
- G02B27/58—Optics for apodization or superresolution; Optical synthetic aperture systems
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0052—Optical details of the image generation
- G02B21/0056—Optical details of the image generation based on optical coherence, e.g. phase-contrast arrangements, interference arrangements
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/008—Details of detection or image processing, including general computer control
Landscapes
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Optics & Photonics (AREA)
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Pathology (AREA)
- Multimedia (AREA)
- Engineering & Computer Science (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Microscoopes, Condenser (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP15190749.0A EP3159728A1 (de) | 2015-10-21 | 2015-10-21 | Interferometrisches stehwellenmikroskop |
EP15190749.0 | 2015-10-21 |
Publications (1)
Publication Number | Publication Date |
---|---|
CN107014786A true CN107014786A (zh) | 2017-08-04 |
Family
ID=54337677
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201610863720.XA Pending CN107014786A (zh) | 2015-10-21 | 2016-09-29 | 驻波干涉显微镜 |
Country Status (4)
Country | Link |
---|---|
US (2) | US9885660B2 (de) |
EP (2) | EP3159728A1 (de) |
JP (1) | JP2017078855A (de) |
CN (1) | CN107014786A (de) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2019134585A1 (zh) * | 2018-01-03 | 2019-07-11 | 宁波纳美致生物科技有限公司 | 一种三通道荧光定位超分辨率生物显微系统及方法 |
CN112639448A (zh) * | 2018-07-03 | 2021-04-09 | 美国卫生和人力服务部 | 利用光开关和驻波照射技术提高显微镜轴向分辨率的系统和方法 |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP3159728A1 (de) * | 2015-10-21 | 2017-04-26 | FEI Company | Interferometrisches stehwellenmikroskop |
Citations (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20050006597A1 (en) * | 2003-07-11 | 2005-01-13 | Carl Zeiss Jena Gmbh | Arrangement for the optical detection of light radiation which is excited and/or backscattered in a specimen with a double-objective arrangement |
US20060291043A1 (en) * | 2001-01-05 | 2006-12-28 | Leica Microsystems Heidelberg Gmbh | Interference microscope, and method for operating an interference microscope |
US20070292015A1 (en) * | 2006-06-20 | 2007-12-20 | Nec Corporation | Method and apparatus for inspecting a pattern |
US20080007248A1 (en) * | 2003-08-22 | 2008-01-10 | Centre National De La Recherche Scientifque-Cnrs | Non-invasive electric-filed-detection device and method |
US20080158551A1 (en) * | 2006-12-21 | 2008-07-03 | Hess Harald F | Systems and methods for 3-dimensional interferometric microscopy |
US20090263002A1 (en) * | 2008-04-17 | 2009-10-22 | Ruprecht-Karls-Universitat Heidelberg | Wave field microscope with sub-wavelength resolution and methods for processing microscopic images to detect objects with sub-wavelength dimensions |
WO2010065538A1 (en) * | 2008-12-02 | 2010-06-10 | The Regents Of The University Of California | Imaging arrangement and microscope |
US20100231922A1 (en) * | 2006-12-21 | 2010-09-16 | Howard Hughes Medical Institute | Three-dimensional interferometric microscopy |
CN103189778A (zh) * | 2010-10-14 | 2013-07-03 | 株式会社尼康 | 结构化照明装置、结构化照明显微镜装置以及面形状测定装置 |
US20140050382A1 (en) * | 2011-01-24 | 2014-02-20 | The Board Of Trustees Of The University Of Illinois | Computational Adaptive Optics for Interferometric Synthetic Aperture Microscopy and Other Interferometric Imaging |
CN103874917A (zh) * | 2011-10-12 | 2014-06-18 | 文塔纳医疗系统公司 | 多焦点干涉图像获取 |
JP5609729B2 (ja) * | 2011-03-18 | 2014-10-22 | 横河電機株式会社 | 顕微鏡装置、観察方法および試料搭載機構 |
CN104541194A (zh) * | 2012-08-16 | 2015-04-22 | 莱卡微系统Cms有限责任公司 | 光学装置和显微镜 |
US20150216407A1 (en) * | 2014-01-31 | 2015-08-06 | University Of Rochester | Measurement of the lipid and aqueous layers of a tear film |
Family Cites Families (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE4040441A1 (de) * | 1990-12-18 | 1992-07-02 | Hell Stefan | Doppelkonfokales rastermikroskop |
US5671085A (en) * | 1995-02-03 | 1997-09-23 | The Regents Of The University Of California | Method and apparatus for three-dimensional microscopy with enhanced depth resolution |
JPH09229861A (ja) * | 1996-02-21 | 1997-09-05 | Bunshi Bio Photonics Kenkyusho:Kk | 蛍光顕微鏡 |
DE10250247B4 (de) * | 2002-10-28 | 2006-06-01 | Leica Microsystems Cms Gmbh | Probenträger für die Mikroskopie und Verfahren zum Anfertigen eines Probenträgers |
NL1027462C2 (nl) | 2004-11-09 | 2006-05-10 | Koninkl Philips Electronics Nv | Werkwijze voor het lokaliseren van fluorescente markers. |
US7599069B2 (en) * | 2005-05-06 | 2009-10-06 | The University Of Chicago | Vector beam generator using a passively phase stable optical interferometer |
US8319181B2 (en) | 2011-01-30 | 2012-11-27 | Fei Company | System and method for localization of large numbers of fluorescent markers in biological samples |
DE102011017046A1 (de) | 2011-04-14 | 2012-10-18 | Till Photonics Gmbh | Umschaltbare Mikroskopanordnung mit mehreren Detektoren |
US8237835B1 (en) * | 2011-05-19 | 2012-08-07 | Aeon Imaging, LLC | Confocal imaging device using spatially modulated illumination with electronic rolling shutter detection |
FR2978254B1 (fr) * | 2011-07-21 | 2014-01-24 | Imagine Optic | Methode et dispositif optique pour la localisation d'une particule en super resolution |
US20140333750A1 (en) * | 2011-12-15 | 2014-11-13 | President And Fellows Of Harvard College | High resolution dual-objective microscopy |
DE102012200344A1 (de) * | 2012-01-11 | 2013-07-11 | Carl Zeiss Microscopy Gmbh | Mikroskopsystem und Verfahren für die 3-D hochauflösende Mikroskopie |
WO2013146365A1 (ja) * | 2012-03-29 | 2013-10-03 | 三洋電機株式会社 | 蛍光検出装置 |
US8872105B2 (en) | 2013-02-19 | 2014-10-28 | Fei Company | In situ reactivation of fluorescence marker |
WO2014152739A2 (en) * | 2013-03-14 | 2014-09-25 | The Regents Of The University Of California | Interferometric focusing of guide-stars for direct wavefront sensing |
EP3159728A1 (de) * | 2015-10-21 | 2017-04-26 | FEI Company | Interferometrisches stehwellenmikroskop |
-
2015
- 2015-10-21 EP EP15190749.0A patent/EP3159728A1/de not_active Withdrawn
-
2016
- 2016-08-24 US US15/246,512 patent/US9885660B2/en active Active
- 2016-08-24 EP EP16185525.9A patent/EP3159729B1/de active Active
- 2016-09-29 CN CN201610863720.XA patent/CN107014786A/zh active Pending
- 2016-10-18 JP JP2016204524A patent/JP2017078855A/ja active Pending
-
2018
- 2018-01-26 US US15/880,945 patent/US10401291B2/en active Active
Patent Citations (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20060291043A1 (en) * | 2001-01-05 | 2006-12-28 | Leica Microsystems Heidelberg Gmbh | Interference microscope, and method for operating an interference microscope |
US20050006597A1 (en) * | 2003-07-11 | 2005-01-13 | Carl Zeiss Jena Gmbh | Arrangement for the optical detection of light radiation which is excited and/or backscattered in a specimen with a double-objective arrangement |
US20080007248A1 (en) * | 2003-08-22 | 2008-01-10 | Centre National De La Recherche Scientifque-Cnrs | Non-invasive electric-filed-detection device and method |
US20070292015A1 (en) * | 2006-06-20 | 2007-12-20 | Nec Corporation | Method and apparatus for inspecting a pattern |
US20100231922A1 (en) * | 2006-12-21 | 2010-09-16 | Howard Hughes Medical Institute | Three-dimensional interferometric microscopy |
US20080158551A1 (en) * | 2006-12-21 | 2008-07-03 | Hess Harald F | Systems and methods for 3-dimensional interferometric microscopy |
US20090263002A1 (en) * | 2008-04-17 | 2009-10-22 | Ruprecht-Karls-Universitat Heidelberg | Wave field microscope with sub-wavelength resolution and methods for processing microscopic images to detect objects with sub-wavelength dimensions |
WO2010065538A1 (en) * | 2008-12-02 | 2010-06-10 | The Regents Of The University Of California | Imaging arrangement and microscope |
CN103189778A (zh) * | 2010-10-14 | 2013-07-03 | 株式会社尼康 | 结构化照明装置、结构化照明显微镜装置以及面形状测定装置 |
US20140050382A1 (en) * | 2011-01-24 | 2014-02-20 | The Board Of Trustees Of The University Of Illinois | Computational Adaptive Optics for Interferometric Synthetic Aperture Microscopy and Other Interferometric Imaging |
JP5609729B2 (ja) * | 2011-03-18 | 2014-10-22 | 横河電機株式会社 | 顕微鏡装置、観察方法および試料搭載機構 |
CN103874917A (zh) * | 2011-10-12 | 2014-06-18 | 文塔纳医疗系统公司 | 多焦点干涉图像获取 |
CN104541194A (zh) * | 2012-08-16 | 2015-04-22 | 莱卡微系统Cms有限责任公司 | 光学装置和显微镜 |
US20150216407A1 (en) * | 2014-01-31 | 2015-08-06 | University Of Rochester | Measurement of the lipid and aqueous layers of a tear film |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2019134585A1 (zh) * | 2018-01-03 | 2019-07-11 | 宁波纳美致生物科技有限公司 | 一种三通道荧光定位超分辨率生物显微系统及方法 |
US10914680B2 (en) | 2018-01-03 | 2021-02-09 | Shenzhen Naguan Biotech Co., Ltd. | Three-channel fluorescence localization super-resolution biological microscope system and method |
CN112639448A (zh) * | 2018-07-03 | 2021-04-09 | 美国卫生和人力服务部 | 利用光开关和驻波照射技术提高显微镜轴向分辨率的系统和方法 |
Also Published As
Publication number | Publication date |
---|---|
US9885660B2 (en) | 2018-02-06 |
US10401291B2 (en) | 2019-09-03 |
US20180195962A1 (en) | 2018-07-12 |
EP3159729A1 (de) | 2017-04-26 |
US20170115224A1 (en) | 2017-04-27 |
JP2017078855A (ja) | 2017-04-27 |
EP3159729B1 (de) | 2023-08-23 |
EP3159728A1 (de) | 2017-04-26 |
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