CN107012198A - A kind of replaceable magnetic frame that pathogen is enriched with for paramagnetic particle method - Google Patents
A kind of replaceable magnetic frame that pathogen is enriched with for paramagnetic particle method Download PDFInfo
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- CN107012198A CN107012198A CN201710375975.6A CN201710375975A CN107012198A CN 107012198 A CN107012198 A CN 107012198A CN 201710375975 A CN201710375975 A CN 201710375975A CN 107012198 A CN107012198 A CN 107012198A
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- base
- magnetic frame
- ndfeb magnet
- replaceable magnetic
- cavity
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- 230000005291 magnetic effect Effects 0.000 title claims abstract description 90
- 238000000034 method Methods 0.000 title claims abstract description 20
- 239000002245 particle Substances 0.000 title claims abstract description 18
- 230000005298 paramagnetic effect Effects 0.000 title claims abstract description 17
- 244000052769 pathogen Species 0.000 title claims abstract description 16
- 230000001717 pathogenic effect Effects 0.000 title claims abstract description 16
- QJVKUMXDEUEQLH-UHFFFAOYSA-N [B].[Fe].[Nd] Chemical compound [B].[Fe].[Nd] QJVKUMXDEUEQLH-UHFFFAOYSA-N 0.000 claims description 3
- 238000005452 bending Methods 0.000 claims description 3
- 229910001172 neodymium magnet Inorganic materials 0.000 claims description 3
- 239000011324 bead Substances 0.000 abstract description 28
- 238000010521 absorption reaction Methods 0.000 abstract description 6
- 238000007689 inspection Methods 0.000 abstract description 6
- 238000001179 sorption measurement Methods 0.000 abstract description 6
- 230000008901 benefit Effects 0.000 abstract description 3
- 230000008859 change Effects 0.000 abstract description 3
- 230000000694 effects Effects 0.000 description 14
- 239000006228 supernatant Substances 0.000 description 14
- 238000010790 dilution Methods 0.000 description 11
- 239000012895 dilution Substances 0.000 description 11
- 239000007788 liquid Substances 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 231100000693 bioaccumulation Toxicity 0.000 description 3
- 230000008033 biological extinction Effects 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 208000015181 infectious disease Diseases 0.000 description 3
- 238000005070 sampling Methods 0.000 description 3
- 241000588724 Escherichia coli Species 0.000 description 2
- 241000191967 Staphylococcus aureus Species 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000006210 lotion Substances 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical compound C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 description 1
- RBTBFTRPCNLSDE-UHFFFAOYSA-N 3,7-bis(dimethylamino)phenothiazin-5-ium Chemical compound C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 RBTBFTRPCNLSDE-UHFFFAOYSA-N 0.000 description 1
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 description 1
- 241001478240 Coccus Species 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 235000009754 Vitis X bourquina Nutrition 0.000 description 1
- 235000012333 Vitis X labruscana Nutrition 0.000 description 1
- 240000006365 Vitis vinifera Species 0.000 description 1
- 235000014787 Vitis vinifera Nutrition 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 239000000443 aerosol Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 229910052796 boron Inorganic materials 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
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- 210000002429 large intestine Anatomy 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 206010025482 malaise Diseases 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 229960000907 methylthioninium chloride Drugs 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
Abstract
The invention discloses a kind of replaceable magnetic frame that pathogen is enriched with for paramagnetic particle method, it includes middle upper supporting surface and base by two symmetrical support connections, wherein, the center of the upper supporting surface is provided with opening, and the upper surface of the base is just provided with aperture to the center of opening；In the lower section of the aperture, the bottom of the base is detachably provided with ndfeb magnet, and the ndfeb magnet is obliquely installed.The replaceable magnetic frame has：1) shorten the enrichment with magnetic bead time, adsorption and enrichment magnetic bead can be such as completed in 3 minutes；2) efficiency that paramagnetic particle method adsorbs pathogen is improved, such as absorption magnetic bead efficiency can reach more than 95%, be prevented effectively from false negative result or missing inspection；3) it is easy to operate, can random interpolation, change usage quantity, the advantages of being convenient for carrying.
Description
Technical field
The present invention relates to detection device technical field, and in particular to a kind of replaceable magnetic that pathogen is enriched with for paramagnetic particle method
Power frame.
Background technology
Bioaerosol is the particulate containing the biological substance such as microorganism or large biological molecule, and its particle diameter is general at 0.5 μm
Between~100 μm, band is infectious, variability and toxicity.By species point, in bioaerosol comprising virus, bacterium, fungi,
Anaphylactogen, endotoxin and polysaccharide etc..Bioaerosol is floated in atmosphere, and sucking lung by respiration forms imbedibility
Infection, can cause a series of malaise symptoms and disease of body, such as anaphylactia, communicable disease, acute respiratory disease
Deng.
The method of sampling of bioaerosol is acted on mainly by means of the external force of vavuum pump, within a certain period of time that biogas is molten
Glue is collected, and then air microbe is enriched with, separate, purify, examine and identified.The kind of current bioaerosol sampler
Class is various, is all that according to certain flow velocity aerosol is struck into specific solid phase adsorption medium based on sampling principle, plays pair
The absorption of bioaerosol.It is high that its advantage is mainly sampling efficiency height, Microbial survival rate.But there is also certain defect, at present
The air microorganism sampler of commercial type is mostly without enrichment function, and the cause of disease bulk concentration collected is low, it is impossible to routine
Molecular biology method is detected.Meanwhile, it is corresponding due to lacking during progress paramagnetic particle method enrichment pathogen after acquisition
Efficient magnetic frame, be arranged in parallel magnet in side or bottom surface mainly due to existing magnetic frame, it there are following problem：
1) magnetic field is not big enough, causes a large amount of magnetic beads not to be enriched with, with the removal of liquid during washing lotion, together with fluid loss, causes enrichment
Efficiency is low, time length, missing inspection the problems such as；2) most of magnetic frame is porous integrated design, non-dismountable, it has not been convenient to Yong Hudan
Use when solely being detected to individual sample；3) collecting pipe and magnetic frame neck tension, are not easily taken out in experiment, easily let out liquid in pipe
Leakage, it is impossible to effectively obtain pathogen, cause the problems such as inaccurate sampled result, missing inspection.
The content of the invention
It is existing to solve it is an object of the invention to provide a kind of replaceable magnetic frame that pathogen is enriched with for paramagnetic particle method
Magnetic frame magnetic field magnetic force is not big enough, causes a large amount of magnetic beads not to be enriched with, and is easily lost in, and causes that bioaccumulation efficiency is low, time-consuming, leakage
Inspection, the technical problem such as be inconvenient to use.
To achieve the above object, the present invention uses following technical scheme：
A kind of replaceable magnetic frame that pathogen is enriched with for paramagnetic particle method, including centre is by two symmetrical supports connections
Upper supporting surface and base, wherein, the center of the upper supporting surface is provided with opening, the center of the upper surface of the base just to opening
Provided with aperture；In the lower section of the aperture, the bottom of the base is detachably provided with ndfeb magnet, and the ndfeb magnet is tilted
Set.
Replaceable magnetic frame as described above, it is preferable that the bottom of the base is provided with cavity, hangs down in the cavity madial wall
Straight upwardly direction is provided with groove, and the upper end of the groove is transversely provided with neck, the size of the ndfeb magnet and the sky
The size of chamber is mutually matched, and the ndfeb magnet is provided with the card engaged with the groove and neck.
Replaceable magnetic frame as described above, it is preferable that the bottom of the base is provided with cavity, the ndfeb magnet is put
In in the cavity, the lower end of the cavity is provided with base cover.
In one embodiment, it is preferable that the base cover is fastened on the lower end of the cavity.
In another embodiment, it is preferable that the base cover is used with the lower end of the cavity and is threadedly coupled.
Replaceable magnetic frame as described above, it is preferable that the angle of the ndfeb magnet and base plane is 0 °~
30 °, it is highly preferred that the angle of the boron magnets and base plane is 10 °.
Replaceable magnetic frame as described above, it is preferable that the ndfeb magnet is replaced by electromagnet, the base
Direct current electric switch provided with the control electromagnet is with exchanging electric switch.
Replaceable magnetic frame as described above, it is preferable that each support is to have to rolled over, support after inside bending
The elasticity inwardly compressed.
Replaceable magnetic frame as described above, it is preferable that the size of the aperture is consistent with centrifuge tube ttom of pipe external diameter.
Replaceable magnetic frame as described above, it is preferable that the profile of the base be triangle, square, regular polygon or
It is circular.Preferably, when being set to triangle, square, regular polygon, its each side is provided with the arc caved inward.
Replaceable magnetic frame as described above, it is preferable that the upper surface of the base is symmetrically arranged with four through holes.
Replaceable magnetic frame as described above, it is preferable that the bottom surface of the base is provided with friction pad.
Replaceable magnetic frame as described above, it is preferable that the upper supporting surface is square, is set on two adjacent outer edges
There is projection, another two adjacent edges are provided with the socket being adapted with the projection.
The present invention's provides the replaceable magnetic frame that paramagnetic particle method is enriched with pathogen, can be solved after collection bioaerosol
Bioaccumulation efficiency is lifted certainly and effectively, it is cost-effective.
It also has the following advantages that：
1) shorten the enrichment with magnetic bead time, adsorption and enrichment magnetic bead can be such as completed in 3 minutes；
2) efficiency that paramagnetic particle method adsorbs pathogen is improved, such as absorption magnetic bead efficiency can reach more than 90%, be prevented effectively from vacation
Negative findings or missing inspection；
3) it is easy to operate, can random interpolation, change usage quantity, be convenient for carrying.
Brief description of the drawings
Fig. 1 is the dimensional structure diagram of one embodiment of the present invention.
Fig. 2 is the dimensional structure diagram of another preferred embodiment of the invention.
Fig. 3 is microscopy results figure in one embodiment of the invention.
Embodiment
It should be noted that in the case where not conflicting, the feature in embodiment and embodiment in the application can phase
Mutually combination.Turn next to accompanying drawing and describe the present invention in detail in conjunction with the embodiments, accompanying drawing is only for reference and explanation is used, not structure
The limitation of paired scope of patent protection of the present invention.
Embodiment 1
In order to solve the magnetic frame of current commercial type, magnetic field is not big enough, causes a large amount of magnetic beads not to be enriched with, easily stream
Lose, cause that bioaccumulation efficiency is low, time-consuming, missing inspection, be inconvenient to use；Meanwhile, also it there are in gatherer process due to washing lotion
Misoperation, causes magnetic bead to be lost in serious, the technical problem such as cause testing result inaccurate, and the invention provides for paramagnetic particle method
It is enriched with the replaceable magnetic frame of pathogen.
Specifically, as shown in figure 1, it includes the middle upper supporting surface 1 and base 3 connected by two symmetrical supports 2, its
In, the center of upper supporting surface 1 is provided with placing the opening of centrifuge tube, and the upper surface of base 3 is just provided with small to the center of opening
The circle tip portion of centrifuge tube ttom of pipe, when the collecting pipe used is the centrifuge tube that bottom is circle tip, can be placed in small by hole 4
Kong Zhong is stable to fix the centrifuge tube.The magnetic field for the magnet that the aperture can also be set bottom, which is exposed, to be come, it is to avoid magnetic force simultaneously hinders
Gear, and reduce concentration effect.In the lower section of the aperture 4, ndfeb magnet, ndfeb magnet are detachably provided with the bottom of base
Plane be obliquely installed, and ndfeb magnet is detachably set, it can be seen that ndfeb magnet from aperture.
The detachable setting of ndfeb magnet can be using following setting：As shown in Figure 2, the bottom of base 3 is provided with cavity
5, the cavity 5 is to be used to place ndfeb magnet, and the inner roof wall of cavity 5 is to be obliquely installed, the connection aperture 4 of cavity 5, in the cavity
The vertical direction from bottom to top of 5 madial walls is provided with groove, and the upper end of groove is provided with neck, ndfeb magnet laterally to the left or to the right
Size and the size of cavity be mutually matched, ndfeb magnet is also provided with the card engaged with groove and neck, the width of card
Degree is equal to or less than the width of groove, and the thickness of card and the width of neck match.The thickness of ndfeb magnet is less than cavity
Height, ndfeb magnet is positioned in cavity, card alignment indentation, reach neck when, rotate ndfeb magnet, make card
It is sticked in neck, snap-in structure can stablize fixed ndfeb magnet, by rotating again detachable ndfeb magnet, realizing can
Dismounting is set.
Ndfeb magnet is obliquely installed, and can be obliquely installed the inner bottom wall of cavity, is placed after ndfeb magnet, neodymium iron boron
The plane of magnet and the horizontal plane of base do not be arranged in parallel, there are certain angle, angle can for 0 °~30 °, i.e. magnetic
When being placed with centrifuge tube on power frame, centrifuge tube ttom of pipe is not vertically arranged with ndfeb magnet, when centrifuge tube ttom of pipe and magnet into
Certain angle.
Also ndfeb magnet can be set to inclined in itself, such as can be by the plane of ndfeb magnet and base plane angle point
0 °, 10 °, 30 ° is not set to, so, is placed after centrifuge tube, centrifuge tube and base plane angle are 90 °, 80 ° 60 °.Take centrifuge tube
In original magnetic bead concentration 10mg/ml, do three parts of Duplicate Samples, the plane of ndfeb magnet be respectively adopted and distinguishes with base plane angle
Magnetic frame for 90 °, 80 ° 60 ° is enriched with, and supernatant is taken after enrichment, detects supernatant with extinction photometer, as a result such as
Shown in table 1, the wherein lower explanation adsorption effect of supernatant is better.
Table 1
| Ttom of pipe and magnet surface gradient | 90° | 80° | 60° |
| Supernatant | 92ng/ml | 37ng/ml | 131ng/ml |
The lower explanation concentration effect of the concentration of magnetic bead is better in supernatant after enrichment, is illustrated by upper table result, works as centrifugation
It is preferable that pipe ttom of pipe is set to 10 ° of concentration effects with magnet into 80 degree of inclination contacts, i.e. ndfeb magnet plane and base plane angle.
In order to be able to make the better of enrichment, the ndfeb magnet in cavity is replaced by electromagnet, on base also
Provided with mutual of size and shape for controlling the direct current electric switch of the electromagnet with exchanging electric switch, electromagnet and above-mentioned cavity
Match somebody with somebody, while provided with card, being embedded in cavity.Turn of magnetic bead in collecting pipe liquid can be changed by convert alternating current electrode direction
Dynamic direction, makes pathogen enrichment more abundant.Meanwhile, connection direct current can be made by the effect of electromagnet formation superparamagnetic
Magnetic bead quick adsorption within a few minutes, to bottom of the tube is collected, efficiently accomplishes at a high speed enrichment in pipe.Electromagnet can change collecting pipe liquid
The rotation direction of magnetic bead is controlled by alternating-current switch in body, can so magnetic bead is well mixed in the solution, and connection direct current is opened
Guan Shi, electromagnet plays magnet, for annex magnetic bead.Such as original magnetic bead concentration 10mg/ml in collecting pipe, when being enriched with different
Between after take supernatant, with extinction photometer detect supernatant, supernatant it is lower explanation adsorption effect it is better.Enrichment is not
The supernatant detected with the time with extinction photometer is as shown in table 2.
Table 2
| Enrichment time | 1 minute | 3 minutes | 5 minutes |
| Supernatant | 4ng/ml | 0ng/ml | 0ng/ml |
As a result illustrate, the replaceable magnetic frame prepared using the present invention can complete enrichment in 3 minutes, accumulation rate can reach
100%.
Two supports 2 of supporting surface 1 and base 3 can be set to be formed after inside bending from top to bottom to rolled over, branch in connection
Frame has the elasticity inwardly compressed.So replaceable magnetic frame is applicable to the centrifuge tube or collecting pipe of different thicknesses, passes through branch
The inside elastic force of frame steps up centrifuge tube or collecting pipe, without departing from magnetic frame.Meanwhile, also make the lighter easy grasping of magnetic frame.
The profile of base can be set to square or circular or other shapes；Four are symmetrically arranged with the upper surface of base to lead to
Hole, the setting of four through holes makes the magnetic force in magnetic field pass through base, the magnetic bead useful effect into centrifuge tube or collecting pipe, it is to avoid because
Base stops a part of magnetic force.When being set to square, its four side is provided with the arc 6 caved inward, and so design makes magnetic frame both beautiful
See and saved cost of manufacture again, enrichment is also effectively improved.In order to be able to be the stable placement of magnetic frame, the bottom surface of base, which is provided with, rubs
Scrubbing pad, friction pad, which can be set, is arranged with three or four.
As shown in Fig. 2 when upper supporting surface 1 is square, circular arc or short side can be set in square sharp corner, it is both beautiful
See again using safety.Four square sides on two adjacent outer edges provided with projection 7, another two adjacent edges be provided with it is described
The socket 8 that projection is adapted, multiple different magnetic frames can so be combined use, can random interpolation, replacing uses
Quantity, and be convenient for carrying.Wherein, the reference 9 of gray bars shown in Fig. 2, is for distinguishing interface.
The magnetic frame prepared using the present invention can effectively improve the effect that paramagnetic particle method adsorbs pathogen, such as absorption magnetic bead efficiency
It can reach 95%.Carry out that there is good concentration effect during microorganism enrichment experiment, such as Escherichia coli solution carries out 10 multiple proportions ladder
Degree dilution, take dilution after sample 1 (dilution gradient is 10-5) (dilution gradient is 10 with sample 2-6), adsorbed using paramagnetic particle method
Escherichia coli, enrichment reaction is carried out using the magnetic frame of the present invention, takes the supernatant after enrichment and enrichment with magnetic bead precipitation to carry out bacterium
Fall culture, obtain total plate count as shown in table 3.
Table 3
| Sample number | Dilution gradient | Supernatant (cfu/mL) | Enrichment with magnetic bead precipitates (cfu/mL) |
| 1 | 10-5 | 19 | 320 |
| 2 | 10-6 | 0 | 52 |
As a result magnetic frame prepared by the present invention is illustrated, with good concentration effect.
Embodiment 2
The present embodiment is that on the basis of embodiment 1, the detachable setting of ndfeb magnet can be using following setting：Base
Bottom be provided with cavity, the lower end of cavity sealed with base cover, and sealed structure can be used：It is provided with the lower end port of cavity
Internal thread, the outer edge of base cover is provided with external screw thread, and ndfeb magnet is built in cavity by screw thread.Sealed structure is also
It can use：Base cover fasten with cavity in, base cover is embedded in cavity, base cover be provided with can be used for rotation back out it is small slit
Mouth or small slot, in this way, both having ensured that bottom surface was flat, can guarantee that base cover is conveniently removed again.
The magnetic frame prepared using the present invention can effectively improve the effect that paramagnetic particle method adsorbs pathogen, such as absorption magnetic bead efficiency
It can reach 96%.Carry out that there is good concentration effect during microorganism enrichment experiment, golden yellow grape is such as adsorbed using paramagnetic particle method
Coccus, staphylococcus aureus solution carry out 10 multiple proportions gradient dilutions, go dilution after sample 1 (dilution gradient is 10-3), sample 2
(dilution gradient is 10-4), (dilution gradient is 10 to sample 3-5), absorption staphylococcus aureus is carried out using paramagnetic particle method, this is utilized
The magnetic frame of embodiment carries out enrichment reaction, takes the supernatant after enrichment and enrichment with magnetic bead precipitation to carry out bacterium colony culture respectively, obtains
Total plate count is obtained as shown in table 4, wherein generally higher than 300 be that can not count more.
Table 4
| Sample number | Dilution gradient | Supernatant (cfu/mL) | Enrichment with magnetic bead precipitates (cfu/mL) |
| 1 | 10-3 | It can not count more | It can not count more |
| 2 | 10-4 | 2 | It can not count more |
| 3 | 10-5 | 0 | 36 |
As a result magnetic frame prepared by the present invention is illustrated, with good concentration effect.
Using micro- sem observation, using the concentration effect after magnetic frame of the present invention, as shown in figure 3, A is large intestine bar in Fig. 3
Bacterium methylene blue is dyed, and B observes figure under the microscope for the capture magnetic bead of blank, and C is that the magnetic frame prepared using invention is captured 3 minutes
Capture magnetic bead afterwards observes figure under the microscope, as a result also illustrates that magnetic frame prepared by the present invention has good enrichment,
Loss of the magnetic bead with liquid can be prevented effectively from, the generation of false negative is also prevented effectively from.
The above described is only a preferred embodiment of the present invention, being not the limitation that other forms are done to the present invention, appoint
What those skilled in the art changed or be modified as possibly also with the technology contents of the disclosure above equivalent variations etc.
Imitate embodiment.But it is every without departing from technical solution of the present invention content, the technical spirit according to the present invention is to above example institute
Any simple modification, equivalent variations and the remodeling made, still fall within the protection domain of technical solution of the present invention.
Claims (10)
1. a kind of replaceable magnetic frame that pathogen is enriched with for paramagnetic particle method, it is characterised in that it includes middle symmetrical by two
Support connection upper supporting surface and base, wherein, the center of the upper supporting surface is provided with opening, and the upper surface of the base is just
Aperture is provided with to the center of opening；In the lower section of the aperture, the bottom of the base is detachably provided with ndfeb magnet, the neodymium
Iron boron magnets are obliquely installed.
2. replaceable magnetic frame as claimed in claim 1, it is characterised in that the bottom of the base is provided with cavity, in the sky
Chamber inner sidewall vertically upward direction is provided with groove, and the upper end of the groove is transversely provided with neck, the ndfeb magnet it is big
The small size with the cavity is mutually matched, and the ndfeb magnet is provided with the card engaged with the groove and neck.
3. replaceable magnetic frame as claimed in claim 1, it is characterised in that the bottom of the base is provided with cavity, the neodymium
Iron boron magnets are placed in the cavity, and the lower end of the cavity is provided with base cover.
4. replaceable magnetic frame as claimed in claim 1, it is characterised in that described be obliquely installed refers to ndfeb magnet and bottom
The angle of seat plane is 0 °~30 °.
5. replaceable magnetic frame as claimed in claim 1, it is characterised in that the ndfeb magnet is replaced by electromagnet,
Direct current electric switch of the base provided with the control electromagnet is with exchanging electric switch.
6. replaceable magnetic frame as claimed in claim 1, it is characterised in that each support is outside after inside bending
Folding, the support has the elasticity inwardly compressed.
7. replaceable magnetic frame as claimed in claim 1, it is characterised in that the profile of the base is triangle, square, just
Polygon or circle.
8. replaceable magnetic frame as claimed in claim 1, it is characterised in that the upper surface of the base is symmetrically arranged with three
Or four through holes.
9. replaceable magnetic frame as claimed in claim 1, it is characterised in that the bottom surface of the base is provided with friction pad.
10. replaceable magnetic frame as claimed in any one of claims 1-9 wherein, it is characterised in that the upper supporting surface be it is square,
Provided with projection on two adjacent outer edges, another two adjacent edges are provided with the socket being adapted with the projection.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710375975.6A CN107012198B (en) | 2017-05-25 | 2017-05-25 | Replaceable magnetic rack for enriching pathogens by magnetic bead method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710375975.6A CN107012198B (en) | 2017-05-25 | 2017-05-25 | Replaceable magnetic rack for enriching pathogens by magnetic bead method |
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| CN107012198B CN107012198B (en) | 2023-12-01 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109022252A (en) * | 2018-08-01 | 2018-12-18 | 杭州富集生物科技有限公司 | A kind of high efficiency large volume bottom magnetic StrongmenGroup acquisition means |
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