CN206736280U - A kind of replaceable magnetic frame for paramagnetic particle method enrichment pathogen - Google Patents
A kind of replaceable magnetic frame for paramagnetic particle method enrichment pathogen Download PDFInfo
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- CN206736280U CN206736280U CN201720588084.4U CN201720588084U CN206736280U CN 206736280 U CN206736280 U CN 206736280U CN 201720588084 U CN201720588084 U CN 201720588084U CN 206736280 U CN206736280 U CN 206736280U
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- magnetic frame
- ndfeb magnet
- replaceable magnetic
- cavity
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Abstract
The utility model discloses a kind of replaceable magnetic frame for paramagnetic particle method enrichment pathogen, it includes middle upper supporting surface and base by two symmetrical support connections, wherein, the center of the upper supporting surface is provided with opening, and the center of the upper surface face opening of the base is provided with aperture；In the lower section of the aperture, the bottom of the base is detachably provided with ndfeb magnet, and the ndfeb magnet is obliquely installed.The replaceable magnetic frame has：1) shorten the enrichment with magnetic bead time, adsorption and enrichment magnetic bead can be such as completed in 3 minutes；2) efficiency of paramagnetic particle method absorption pathogen is improved, magnetic bead efficiency is such as adsorbed and can reach more than 95%, effectively avoid false negative result or missing inspection；3) it is easy to operate, can random interpolation, change usage quantity, the advantages that being convenient for carrying.
Description
Technical field
The utility model is related to detection device technical field, and in particular to it is a kind of for paramagnetic particle method enrichment pathogen can be more
Change magnetic frame.
Background technology
Bioaerosol is the particulate containing the biological substance such as microorganism or large biological molecule, and its particle diameter is typically at 0.5 μm
Between~100 μm, band is infectious, variability and toxicity.By species point, in bioaerosol comprising virus, bacterium, fungi,
Anaphylactogen, endotoxin and polysaccharide etc..Bioaerosol floats in atmosphere, and sucking lung by respiration forms imbedibility
Infection, a series of malaise symptoms and disease of body can be caused, such as anaphylactia, communicable disease, acute respiratory disease
Deng.
The method of sampling of bioaerosol acts on mainly by means of the external force of vavuum pump, within a certain period of time that biogas is molten
Glue is collected, and then air microbe is enriched with, separate, purify, examine and identified.The kind of bioaerosol sampler at present
Class is various, is all that aerosol is struck into specific solid phase adsorption medium according to certain flow velocity based on sampling principle, plays pair
The absorption of bioaerosol.Its advantage is mainly that sampling efficiency is high, Microbial survival rate is high.But there is also certain defect, at present
The air microorganism sampler of commercial type does not have enrichment function mostly, and the cause of disease bulk concentration collected is low, can not use conventional
Molecular biology method is detected.It is meanwhile corresponding due to lacking during progress paramagnetic particle method enrichment pathogen after acquisition
Efficient magnetic frame, be arranged in parallel mainly due to existing magnetic frame in side or bottom surface magnet, it there are following problem：
1) magnetic field is not big enough, causes a large amount of magnetic beads not to be enriched with, and with the removal of liquid during washing lotion, together with fluid loss, causes to be enriched with
The problems such as efficiency is low, the time is long, missing inspection；2) most of magnetic frame is porous integrated design, non-dismountable, it has not been convenient to Yong Hudan
Use when solely being detected to individual sample；3) collecting pipe and magnetic frame neck tension, are not easily taken out in experiment, easily let out liquid in pipe
Leakage, can not effectively obtain pathogen, the problems such as causing sampled result inaccurate, missing inspection.
The content of the invention
The purpose of this utility model is to provide a kind of replaceable magnetic frame for paramagnetic particle method enrichment pathogen, to solve
Existing magnetic frame magnetic field magnetic force is not big enough, causes a large amount of magnetic beads not to be enriched with, and is easily lost in, and causes bioaccumulation efficiency low, time-consuming
Length, missing inspection, the technical problem such as be inconvenient to use.
To achieve the above object, the utility model uses following technical scheme：
A kind of replaceable magnetic frame for paramagnetic particle method enrichment pathogen, including centre is by two symmetrical supports connections
Upper supporting surface and base, wherein, the center of the upper supporting surface is provided with opening, the center of the upper surface face opening of the base
Provided with aperture；In the lower section of the aperture, the bottom of the base is detachably provided with ndfeb magnet, and the ndfeb magnet tilts
Set.
Replaceable magnetic frame as described above, it is preferable that the bottom of the base is provided with cavity, is hung down in the cavity madial wall
Straight upwardly direction is provided with groove, and the upper end of the groove is transversely provided with neck, the size of the ndfeb magnet and the sky
The size of chamber is mutually matched, and the ndfeb magnet is provided with the card engaged with the groove and neck.
Replaceable magnetic frame as described above, it is preferable that the bottom of the base is provided with cavity, and the ndfeb magnet is put
In in the cavity, the lower end of the cavity is provided with base cover.
In one embodiment, it is preferable that the base cover is fastened on the lower end of the cavity.
In another embodiment, it is preferable that the base cover is used with the lower end of the cavity and is threadedly coupled.
Replaceable magnetic frame as described above, it is preferable that the angle of the ndfeb magnet and base plane is 0 °~
30 °, it is highly preferred that the angle of the boron magnets and base plane is 10 °.
Replaceable magnetic frame as described above, it is preferable that the ndfeb magnet is replaced by electromagnet, on the base
Direct current electric switch provided with the control electromagnet is with exchanging electric switch.
Replaceable magnetic frame as described above, it is preferable that each support is that support has to rolled over after inside bending
The elasticity inwardly compressed.
Replaceable magnetic frame as described above, it is preferable that the size of the aperture is consistent with centrifuge tube ttom of pipe external diameter.
Replaceable magnetic frame as described above, it is preferable that the profile of the base be triangle, square, regular polygon or
It is circular.Preferably, when being set to triangle, square, regular polygon, its each side is provided with the arc to cave inward.
Replaceable magnetic frame as described above, it is preferable that the upper surface of the base is symmetrically arranged with four through holes.
Replaceable magnetic frame as described above, it is preferable that the bottom surface of the base is provided with friction pad.
Replaceable magnetic frame as described above, it is preferable that the upper supporting surface is square, is set on two adjacent outer edges
There is projection, another two adjacent edges are provided with the socket being adapted with the projection.
The replaceable magnetic frame of the present utility model for providing paramagnetic particle method enrichment pathogen, the energy after bioaerosol is gathered
It is enough to solve and effectively lift bioaccumulation efficiency, it is cost-effective.
It also has the following advantages that：
1) shorten the enrichment with magnetic bead time, adsorption and enrichment magnetic bead can be such as completed in 3 minutes；
2) efficiency of paramagnetic particle method absorption pathogen is improved, magnetic bead efficiency is such as adsorbed and can reach more than 90%, effectively avoid vacation
Negative findings or missing inspection；
3) it is easy to operate, can random interpolation, change usage quantity, be convenient for carrying.
Brief description of the drawings
Fig. 1 is the dimensional structure diagram of the preferred embodiment of the utility model one.
Fig. 2 is the dimensional structure diagram of another preferred embodiment of the utility model.
Fig. 3 is microscopy results figure in the embodiment of the utility model one.
Embodiment
It should be noted that in the case where not conflicting, the feature in embodiment and embodiment in the application can phase
Mutually combination.Turning next to accompanying drawing and describe the utility model in detail in conjunction with the embodiments, accompanying drawing is only for reference and explanation uses,
The limitation to the utility model patent protection domain is not formed.
Embodiment 1
In order to solve the magnetic frame of current commercial type, magnetic field is not big enough, causes a large amount of magnetic beads not to be enriched with, easily stream
Lose, cause that bioaccumulation efficiency is low, time-consuming, missing inspection, is inconvenient to use；Meanwhile also it there are in gatherer process due to washing lotion
Misoperation, cause magnetic bead to be lost in serious, cause the technical problems such as testing result inaccuracy, the utility model is provided for magnetic
Pearl method is enriched with the replaceable magnetic frame of pathogen.
Specifically, as shown in figure 1, it includes the middle upper supporting surface 1 and base 3 connected by two symmetrical supports 2, its
In, the center of upper supporting surface 1 is provided with placing the opening of centrifuge tube, and the center of the upper surface face opening of base 3 is provided with small
Hole 4, when the collecting pipe used is the centrifuge tube that bottom is circle tip, the circle tip portion of centrifuge tube ttom of pipe can be placed in small
Kong Zhong is stable to fix the centrifuge tube.The magnetic field for the magnet that the aperture can also be set bottom, which exposes, to be come, and is avoided magnetic force and is hindered
Gear, and reduce concentration effect.In the lower section of the aperture 4, ndfeb magnet, ndfeb magnet are detachably provided with the bottom of base
Plane be obliquely installed, and ndfeb magnet is detachably set, it can be seen that ndfeb magnet from aperture.
The detachable setting of ndfeb magnet can use following set：As shown in Figure 2, the bottom of base 3 is provided with cavity
5, the cavity 5 is to be used to place ndfeb magnet, and the inner roof wall of cavity 5 is to be obliquely installed, and cavity 5 connects aperture 4, in the cavity
The vertical direction from bottom to top of 5 madial walls is provided with groove, and the upper end of groove is provided with neck, ndfeb magnet laterally to the left or to the right
Size and the size of cavity be mutually matched, ndfeb magnet is also provided with the card engaged with groove and neck, the width of card
Equal to or less than the width of groove, the thickness of card and the width of neck match degree.The thickness of ndfeb magnet is less than cavity
Height, ndfeb magnet is positioned in cavity, card alignment indentation, reach neck when, rotate ndfeb magnet, make card
It is sticked in neck, snap-in structure can stablize fixed ndfeb magnet, and by rotation and detachable ndfeb magnet, realizing can
Dismounting is set.
Ndfeb magnet is obliquely installed, and can be obliquely installed the inner bottom wall of cavity, after placing ndfeb magnet, neodymium iron boron
The plane of magnet and the horizontal plane of base do not be arranged in parallel, there are certain angle, and angle can be 0 °~30 °, i.e. magnetic
When being placed with centrifuge tube on power frame, centrifuge tube ttom of pipe is not vertically arranged with ndfeb magnet, when centrifuge tube ttom of pipe and magnet into
Certain angle.
Also ndfeb magnet can be set to inclined in itself, such as can be by the plane of ndfeb magnet and base plane angle point
0 °, 10 °, 30 ° is not set to, and so, after placing centrifuge tube, centrifuge tube and base plane angle are 90 °, 80 ° 60 °.Take centrifuge tube
In original magnetic bead concentration 10mg/ml, do three parts of Duplicate Samples, the plane of ndfeb magnet be respectively adopted and distinguishes with base plane angle
Magnetic frame for 90 °, 80 ° 60 ° is enriched with, and supernatant is taken after enrichment, detects supernatant with extinction photometer, as a result such as
Shown in table 1, the wherein lower explanation adsorption effect of supernatant is better.
Table 1
| Ttom of pipe and magnet surface gradient | 90° | 80° | 60° |
| Supernatant | 92ng/ml | 37ng/ml | 131ng/ml |
The lower explanation concentration effect of the concentration of magnetic bead is better in supernatant after enrichment, is illustrated by upper table result, works as centrifugation
Pipe ttom of pipe and magnet are into 80 degree of inclination contacts, i.e., it is preferable with base plane angle to be set to 10 ° of concentration effects for ndfeb magnet plane.
In order to make the better of enrichment, the ndfeb magnet in cavity is replaced by electromagnet, on base also
Provided with the direct current electric switch for controlling the electromagnet and exchange electric switch, mutual of the size and shape of electromagnet and above-mentioned cavity
Match somebody with somebody, while be provided with card, be embedded in cavity.Turn of magnetic bead in collecting pipe liquid can be changed by convert alternating current electrode direction
Dynamic direction, make pathogen enrichment more abundant.Meanwhile the effect that direct current forms superparamagnetic field by electromagnet is connected, it can make
Magnetic bead quick adsorption within a few minutes to bottom of the tube is collected, efficiently accomplishes at a high speed enrichment in pipe.Electromagnet can change collecting pipe liquid
The rotation direction of magnetic bead is controlled by alternating-current switch in body, can so magnetic bead is well mixed in the solution, and connection direct current is opened
Guan Shi, electromagnet play magnet, for annex magnetic bead.Such as original magnetic bead concentration 10mg/ml in collecting pipe, when being enriched with different
Between after take supernatant, with extinction photometer detect supernatant, supernatant it is lower explanation adsorption effect it is better.Enrichment is not
The supernatant detected with the time with extinction photometer is as shown in table 2.
Table 2
| Enrichment time | 1 minute | 3 minutes | 5 minutes |
| Supernatant | 4ng/ml | 0ng/ml | 0ng/ml |
As a result illustrate, the replaceable magnetic frame prepared using the utility model can complete enrichment in 3 minutes, and accumulation rate can
Reach 100%.
Two supports 2 of supporting surface 1 and base 3 can be set to be formed after inside bending from top to bottom to rolled over, branch in connection
Frame has the elasticity inwardly compressed.So replaceable magnetic frame is applicable to the centrifuge tube or collecting pipe of different thicknesses, passes through branch
The inside elastic force of frame steps up centrifuge tube or collecting pipe, without departing from magnetic frame.Meanwhile also make magnetic frame is lighter easily to grasp.
The profile of base can be set to square or circular or other shapes；Four are symmetrically arranged with the upper surface of base to lead to
Hole, the setting of four through holes, the magnetic force in magnetic field is passed through base, the magnetic bead useful effect into centrifuge tube or collecting pipe, avoid because
Base stops a part of magnetic force.When being set to square, its four side is provided with the arc 6 to cave inward, and so design makes magnetic frame both beautiful
See and saved cost of manufacture again, enrichment also effectively improves.Placed in order to be that magnetic frame is stable, the bottom surface of base, which is provided with, rubs
Scrubbing pad, friction pad, which can be set, is arranged with three or four.
As shown in Fig. 2 when upper supporting surface 1 is square, circular arc or short side can be set in square sharp corner, it is both beautiful
See again safe to use.Four square sides on two adjacent outer edges be provided with projection 7, another two adjacent edges be provided with it is described
The socket 8 that projection is adapted, can so combine multiple different magnetic frames use, can random interpolation, change use
Quantity, and be convenient for carrying.Wherein, the reference 9 of gray bars shown in Fig. 2, is for distinguishing interface.
The magnetic frame prepared using the utility model can effectively improve the effect of paramagnetic particle method absorption pathogen, such as adsorb magnetic bead
Efficiency can reach 95%.Carry out that there is good concentration effect during microorganism enrichment experiment, as Escherichia coli solution carries out 10 times
Than gradient dilution, (the dilution gradient 10 of sample 1 after dilution is taken-5) and (dilution gradient 10 of sample 2-6), carried out using paramagnetic particle method
Escherichia coli are adsorbed, enrichment reaction is carried out using magnetic frame of the present utility model, take the supernatant after enrichment and enrichment with magnetic bead to sink
Form sediment and carry out bacterium colony culture, it is as shown in table 3 to obtain total plate count.
Table 3
| Sample number | Dilution gradient | Supernatant (cfu/mL) | Enrichment with magnetic bead precipitates (cfu/mL) |
| 1 | 10-5 | 19 | 320 |
| 2 | 10-6 | 0 | 52 |
As a result illustrate magnetic frame prepared by the utility model, there is good concentration effect.
Embodiment 2
The present embodiment is on the basis of embodiment 1, and the detachable setting of ndfeb magnet can use following set：Base
Bottom be provided with cavity, the lower end of cavity is sealed with base cover, and the structure of sealing can use：It is provided with the lower end port of cavity
Internal thread, the outer edge of base cover are provided with external screw thread, ndfeb magnet are built in cavity by screw thread.The structure of sealing is also
It can use：In base cover fastening and cavity, base cover is embedded in cavity, and base cover is small open-minded provided with can be used for rotation to back out
Mouth or small slot, in this way, both having ensured that bottom surface was flat, and can ensures that base cover is conveniently removed.
The magnetic frame prepared using the utility model can effectively improve the effect of paramagnetic particle method absorption pathogen, such as adsorb magnetic bead
Efficiency can reach 96%.Carry out that there is good concentration effect during microorganism enrichment experiment, it is such as golden yellow using paramagnetic particle method absorption
Staphylococcus, staphylococcus aureus solution carry out 10 multiple proportions gradient dilutions, go (the dilution gradient 10 of sample 1 after dilution-3), sample
(the dilution gradient 10 of product 2-4), (dilution gradient 10 of sample 3-5), absorption staphylococcus aureus is carried out using paramagnetic particle method, profit
Enrichment reaction is carried out with the magnetic frame of the present embodiment, takes the supernatant after enrichment and enrichment with magnetic bead precipitation to carry out bacterium colony training respectively
Support, acquisition total plate count is as shown in table 4, wherein generally higher than 300 be that can not count more.
Table 4
| Sample number | Dilution gradient | Supernatant (cfu/mL) | Enrichment with magnetic bead precipitates (cfu/mL) |
| 1 | 10-3 | It can not count more | It can not count more |
| 2 | 10-4 | 2 | It can not count more |
| 3 | 10-5 | 0 | 36 |
As a result illustrate magnetic frame prepared by the utility model, there is good concentration effect.
Using micro- sem observation, using the concentration effect after the utility model magnetic frame, as shown in figure 3, A is big in Fig. 3
Enterobacteria methylene blue dyes, and B is that the capture magnetic bead of blank observes figure under the microscope, and C is the magnetic frame prepared using utility model
Capture magnetic bead after capturing 3 minutes observes figure under the microscope, as a result also illustrates that magnetic frame prepared by the utility model has very
Good enrichment, can effectively avoid loss of the magnetic bead with liquid, also effectively avoid the generation of false negative.
It is described above, only it is preferred embodiment of the present utility model, is not that other forms are done to the utility model
Limitation, any person skilled in the art is changed or is modified as possibly also with the technology contents of the disclosure above equivalent
The equivalent embodiment of change.But it is every without departing from the content of the technical scheme of the utility model, it is real according to technology of the present utility model
Any simple modification, equivalent variations and the remodeling that confrontation above example is made, still fall within the guarantor of technical solutions of the utility model
Protect scope.
Claims (10)
1. a kind of replaceable magnetic frame for paramagnetic particle method enrichment pathogen, it is characterised in that it includes middle symmetrical by two
Support connection upper supporting surface and base, wherein, the center of the upper supporting surface is provided with opening, and the upper surface of the base is just
Aperture is provided with to the center of opening；In the lower section of the aperture, the bottom of the base is detachably provided with ndfeb magnet, the neodymium
Iron boron magnets are obliquely installed.
2. replaceable magnetic frame as claimed in claim 1, it is characterised in that the bottom of the base is provided with cavity, in the sky
Chamber inner sidewall vertically upward direction is provided with groove, and the upper end of the groove is transversely provided with neck, the ndfeb magnet it is big
The small size with the cavity is mutually matched, and the ndfeb magnet is provided with the card engaged with the groove and neck.
3. replaceable magnetic frame as claimed in claim 1, it is characterised in that the bottom of the base is provided with cavity, the neodymium
Iron boron magnets are placed in the cavity, and the lower end of the cavity is provided with base cover.
4. replaceable magnetic frame as claimed in claim 1, it is characterised in that described be obliquely installed refers to ndfeb magnet and bottom
The angle of seat plane is 0 °~30 °.
5. replaceable magnetic frame as claimed in claim 1, it is characterised in that the ndfeb magnet is replaced by electromagnet,
The base is provided with the direct current electric switch of the control electromagnet with exchanging electric switch.
6. replaceable magnetic frame as claimed in claim 1, it is characterised in that each support is outside after inside bending
Folding, the support have the elasticity inwardly compressed.
7. replaceable magnetic frame as claimed in claim 1, it is characterised in that the profile of the base is triangle, square, just
Polygon or circle.
8. replaceable magnetic frame as claimed in claim 1, it is characterised in that the upper surface of the base is symmetrically arranged with three
Or four through holes.
9. replaceable magnetic frame as claimed in claim 1, it is characterised in that the bottom surface of the base is provided with friction pad.
10. replaceable magnetic frame as claimed in any one of claims 1-9 wherein, it is characterised in that the upper supporting surface be it is square,
Projection is provided with two adjacent outer edges, another two adjacent edges are provided with the socket being adapted with the projection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201720588084.4U CN206736280U (en) | 2017-05-25 | 2017-05-25 | A kind of replaceable magnetic frame for paramagnetic particle method enrichment pathogen |
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| CN201720588084.4U CN206736280U (en) | 2017-05-25 | 2017-05-25 | A kind of replaceable magnetic frame for paramagnetic particle method enrichment pathogen |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107012198A (en) * | 2017-05-25 | 2017-08-04 | 杭州富集生物科技有限公司 | A kind of replaceable magnetic frame that pathogen is enriched with for paramagnetic particle method |
-
2017
- 2017-05-25 CN CN201720588084.4U patent/CN206736280U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107012198A (en) * | 2017-05-25 | 2017-08-04 | 杭州富集生物科技有限公司 | A kind of replaceable magnetic frame that pathogen is enriched with for paramagnetic particle method |
| CN107012198B (en) * | 2017-05-25 | 2023-12-01 | 杭州富集生物科技有限公司 | Replaceable magnetic rack for enriching pathogens by magnetic bead method |
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