CN107012198B - Replaceable magnetic rack for enriching pathogens by magnetic bead method - Google Patents
Replaceable magnetic rack for enriching pathogens by magnetic bead method Download PDFInfo
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- CN107012198B CN107012198B CN201710375975.6A CN201710375975A CN107012198B CN 107012198 B CN107012198 B CN 107012198B CN 201710375975 A CN201710375975 A CN 201710375975A CN 107012198 B CN107012198 B CN 107012198B
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- 239000011324 bead Substances 0.000 title claims abstract description 44
- 238000000034 method Methods 0.000 title claims abstract description 19
- 244000052769 pathogen Species 0.000 title claims abstract description 19
- 229910001172 neodymium magnet Inorganic materials 0.000 claims abstract description 37
- 230000006835 compression Effects 0.000 claims description 3
- 238000007906 compression Methods 0.000 claims description 3
- 238000001179 sorption measurement Methods 0.000 abstract description 10
- 238000001514 detection method Methods 0.000 abstract description 6
- 230000001717 pathogenic effect Effects 0.000 abstract description 6
- 238000004904 shortening Methods 0.000 abstract description 2
- 230000000694 effects Effects 0.000 description 15
- 239000006228 supernatant Substances 0.000 description 14
- 239000012895 dilution Substances 0.000 description 10
- 238000010790 dilution Methods 0.000 description 10
- 239000007788 liquid Substances 0.000 description 9
- 239000000443 aerosol Substances 0.000 description 6
- 239000000523 sample Substances 0.000 description 6
- 244000005700 microbiome Species 0.000 description 5
- QJVKUMXDEUEQLH-UHFFFAOYSA-N [B].[Fe].[Nd] Chemical compound [B].[Fe].[Nd] QJVKUMXDEUEQLH-UHFFFAOYSA-N 0.000 description 4
- 230000005389 magnetism Effects 0.000 description 4
- 238000005070 sampling Methods 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 241000588724 Escherichia coli Species 0.000 description 3
- 241000191967 Staphylococcus aureus Species 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000013049 sediment Substances 0.000 description 3
- 230000009295 sperm incapacitation Effects 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- 230000008859 change Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000031700 light absorption Effects 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 239000013566 allergen Substances 0.000 description 1
- 208000026935 allergic disease Diseases 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 229910052796 boron Inorganic materials 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000012470 diluted sample Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000002158 endotoxin Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 208000023504 respiratory system disease Diseases 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Pathology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The application discloses a replaceable magnetic rack for enriching pathogens by a magnetic bead method, which comprises an upper supporting surface and a base, wherein the middle of the upper supporting surface is connected by two symmetrical brackets, an opening is formed in the center of the upper supporting surface, and a small hole is formed in the center of the upper surface of the base, which is opposite to the opening; and a neodymium-iron-boron magnet is detachably arranged at the bottom of the base below the small hole, and is obliquely arranged. The replaceable magnetic rack has: 1) Shortening the magnetic bead enrichment time, for example, the adsorption enrichment of the magnetic beads can be completed within 3 minutes; 2) The pathogen adsorption efficiency of the magnetic bead method is improved, for example, the pathogen adsorption efficiency can reach more than 95%, and false negative results or missed detection can be effectively avoided; 3) Easy operation, convenient carrying and the like, and can be spliced and inserted at will, and the use quantity is changed.
Description
Technical Field
The application relates to the technical field of detection equipment, in particular to a replaceable magnetic frame for enriching pathogens by a magnetic bead method.
Background
Bioaerosols are particles containing biological substances such as microorganisms or biomacromolecules, and generally have particle diameters of 0.5-100 μm and have infectivity, variability and toxicity. The bioaerosols include viruses, bacteria, fungi, allergens, endotoxins, polysaccharides, and the like, according to species. The bioaerosol floats in the air, and inhaled into the lungs through respiration to form inhaled infections, which can cause a series of uncomfortable symptoms and diseases of the organism, such as allergic diseases, infectious diseases, acute respiratory diseases, and the like.
The method for sampling the biological aerosol mainly collects the biological aerosol within a certain time by means of the external force action of a vacuum pump, and then enriches, separates, purifies, checks and identifies air microorganisms. At present, the biological aerosol samplers are various in variety, and based on a sampling principle, aerosol is impacted to a specific solid-phase adsorption medium according to a certain flow rate, so that the biological aerosol is adsorbed. Its advantages are high sampling efficiency and high survival rate of microbe. However, there is a certain defect that most of air microorganism samplers sold in the market at present have no enrichment function, the concentration of the collected pathogens is low, and the collected pathogens cannot be detected by a conventional molecular biological method. Meanwhile, due to the lack of a corresponding high-efficiency magnetic rack in the process of enriching pathogens by a magnetic bead method after collection, the existing magnetic rack is provided with magnets in parallel on the side surface or the bottom surface, and the following problems exist: 1) The insufficient magnetic field causes that a large number of magnetic beads cannot be enriched, washing liquid is removed along with liquid, and the washing liquid is lost along with the liquid, so that the problems of low enrichment efficiency, long time, omission and the like are caused; 2) Most magnetic force frames are of a multi-hole integrated design, are not detachable, and are inconvenient for users to use when detecting individual samples independently; 3) The collecting pipe is too tight with the magnetic frame draw-in groove, is difficult for taking out in the experiment, makes intraductal liquid leak easily, can't effectively acquire the pathogen, causes the sampling result inaccurate, leaks the scheduling problem of examining.
Disclosure of Invention
The application aims to provide a replaceable magnetic rack for enriching pathogens by a magnetic bead method, which aims to solve the technical problems of low enrichment efficiency, long time consumption, missed detection, inconvenient use and the like caused by the fact that a large number of magnetic beads cannot be enriched and are easy to run off due to insufficient magnetic force of a magnetic field of the conventional magnetic rack.
In order to achieve the above purpose, the present application adopts the following technical scheme:
a replaceable magnetic rack for enriching pathogens by a magnetic bead method comprises an upper supporting surface and a base, wherein the middle of the upper supporting surface is connected by two symmetrical brackets, an opening is formed in the center of the upper supporting surface, and a small hole is formed in the center of the upper surface of the base, opposite to the opening; and a neodymium-iron-boron magnet is detachably arranged at the bottom of the base below the small hole, and is obliquely arranged.
As described above, preferably, the bottom of the base is provided with a cavity, a groove is formed in the vertical upward direction of the inner side wall of the cavity, a clamping groove is formed in the upper end of the groove, the size of the neodymium-iron-boron magnet is matched with the size of the cavity, and a card clamped with the groove and the clamping groove is arranged on the neodymium-iron-boron magnet.
Preferably, the bottom of the base is provided with a cavity, the neodymium-iron-boron magnet is arranged in the cavity, and the lower end of the cavity is provided with a base cover.
In one embodiment, the base cover is preferably fastened to the lower end of the cavity.
In another embodiment, the base cover is preferably screwed to the lower end of the cavity.
Preferably, the included angle between the neodymium-iron-boron magnet and the base plane is 0-30 degrees, and more preferably, the included angle between the boron magnet and the base plane is 10 degrees.
Preferably, the neodymium-iron-boron magnet is replaced by an electromagnet, and the base is provided with a direct current switch and an alternating current switch for controlling the electromagnet.
The replaceable magnetic rack is preferably characterized in that each bracket is folded inwards and outwards after being folded inwards, and the brackets have elasticity of inward compression.
The replaceable magnetic rack as described above, preferably, the size of the small hole is consistent with the outside diameter of the bottom of the centrifuge tube.
The replaceable magnetic rack as described above, preferably, the shape of the base is triangle, square, regular polygon or circle. Preferably, when the triangular, square or regular polygon is formed, each side of the triangular, square or regular polygon is provided with an inward concave arc.
The replaceable magnetic rack as described above, preferably, the upper surface of the base is symmetrically provided with four through holes.
The replaceable magnetic stand as described above, preferably, the bottom surface of the base is provided with a friction pad.
As mentioned above, preferably, the upper supporting surface is square, and two adjacent outer edges are provided with protrusions, and the other two adjacent edges are provided with sockets adapted to the protrusions.
The application provides a replaceable magnetic rack for enriching pathogens by a magnetic bead method, which can solve the problem after collecting biological aerosol, effectively improve the enrichment efficiency and save the cost.
It also has the following advantages:
1) Shortening the magnetic bead enrichment time, for example, the adsorption enrichment of the magnetic beads can be completed within 3 minutes;
2) The pathogen adsorption efficiency of the magnetic bead method is improved, for example, the pathogen adsorption efficiency can reach more than 90%, and false negative results or missed detection can be effectively avoided;
3) Easy to operate, can be spliced and inserted at will, and is convenient to carry.
Drawings
Fig. 1 is a schematic perspective view of a preferred embodiment of the present application.
Fig. 2 is a schematic perspective view of another preferred embodiment of the present application.
Fig. 3 is a view of a microscope observation according to an embodiment of the present application.
Detailed Description
It should be noted that, without conflict, the embodiments of the present application and features of the embodiments may be combined with each other. The application will now be described in detail with reference to the drawings, which are provided for reference and illustration only, and are not intended to limit the scope of the application.
Example 1
In order to solve the problems that a large number of magnetic beads cannot be enriched due to insufficient magnetic fields of magnetic frames sold in the market at present, the magnetic beads are easy to run off, so that the enrichment efficiency is low, the time consumption is long, the detection is omitted, the use is inconvenient, and the like; meanwhile, the technical problems of serious magnetic bead loss, inaccurate detection result and the like caused by improper operation of washing liquid in the collection process also exist.
Specifically, as shown in fig. 1, the centrifugal tube comprises an upper supporting surface 1 and a base 3, wherein the middle of the upper supporting surface 1 is connected by two symmetrical brackets 2, an opening for placing a centrifugal tube is arranged in the center of the upper supporting surface 1, a small hole 4 is arranged in the center of the upper surface of the base 3, which is opposite to the opening, and when the adopted collecting tube is a centrifugal tube with a round tip at the bottom, the round tip part of the bottom of the centrifugal tube can be placed in the small hole to stably fix the centrifugal tube. The small holes can expose the magnetic field of the magnet arranged at the bottom, so that the magnetic force is avoided and blocked, and the enrichment effect is reduced. Below this aperture 4, can dismantle in the bottom of base and be equipped with the neodymium iron boron magnetism nature, the plane of neodymium iron boron magnetism nature is the slope setting to the neodymium iron boron magnetism nature can dismantle the setting, can see the neodymium iron boron magnetism nature in the aperture.
The detachable setting of the neodymium-iron-boron magnet can be set as follows: as shown in fig. 2, the bottom of the base 3 is provided with a cavity 5, the cavity 5 is used for placing a neodymium iron boron magnet, the inner top wall of the cavity 5 is obliquely provided, the cavity 5 is communicated with a small hole 4, a groove is formed in the inner side wall of the cavity 5 in the vertical direction from bottom to top, the upper end of the groove is transversely provided with a clamping groove leftwards or rightwards, the size of the neodymium iron boron magnet is mutually matched with that of the cavity, the neodymium iron boron magnet is further provided with a card clamped with the groove and the clamping groove, the width of the card is equal to or smaller than that of the groove, and the thickness of the card is matched with that of the clamping groove. The thickness of neodymium iron boron magnet is less than the height of cavity, places neodymium iron boron magnet in the cavity, and the card aims at the recess, and when reaching the draw-in groove, rotatory neodymium iron boron magnet makes the card block in the draw-in groove, and the block structure can stabilize fixed neodymium iron boron magnet, can dismantle neodymium iron boron magnet again through the rotation, has realized dismantling the setting.
The neodymium iron boron magnet slope sets up, can set up the interior bottom wall slope of cavity, places behind the neodymium iron boron magnet, and the plane of neodymium iron boron magnet and the horizontal plane of base are not parallel arrangement, have certain contained angle, and the contained angle can be 0 ~ 30, has placed the centrifuging tube on the magnetic force frame promptly, and centrifuging tube bottom is not perpendicular setting with neodymium iron boron magnet, becomes certain angle when centrifuging tube bottom and magnet.
The neodymium-iron-boron magnet can be inclined, for example, the included angles between the plane of the neodymium-iron-boron magnet and the plane of the base are respectively 0 degrees, 10 degrees and 30 degrees, and after the centrifugal tube is placed, the included angles between the centrifugal tube and the plane of the base are 90 degrees and 80 degrees and 60 degrees. Taking 10mg/ml of original magnetic beads in a centrifuge tube, performing three parallel samples, enriching by adopting magnetic force frames with angles of 90 degrees and 80 degrees of 60 degrees between the plane of a neodymium iron boron magnet and the plane of a base, taking supernatant after enrichment, and detecting the concentration of the supernatant by using a light absorption photometer, wherein the lower the concentration of the supernatant is, the better the adsorption effect is shown in a table 1.
TABLE 1
| Inclination of pipe bottom and magnet surface | 90° | 80° | 60° |
| Concentration of supernatant | 92ng/ml | 37ng/ml | 131ng/ml |
The lower the concentration of the magnetic beads in the enriched supernatant fluid is, the better the enrichment effect is, and the results of the table show that when the bottom of the centrifuge tube is in inclined contact with the magnet at 80 degrees, namely, the included angle between the plane of the NdFeB magnet and the plane of the base is set to be 10 degrees, the better enrichment effect is achieved.
In order to enable the enrichment effect to be better, the neodymium iron boron magnet in the cavity can be replaced by an electromagnet, a direct current switch and an alternating current switch for controlling the electromagnet are further arranged on the base, the electromagnet is matched with the cavity in size and shape, and meanwhile, a card is arranged and embedded in the cavity. The rotating direction of the magnetic beads in the liquid in the collecting pipe can be changed by alternating current to change the direction of the electrodes, so that the pathogen enrichment is more sufficient. Meanwhile, the direct current is connected to form a superparamagnetic field through the electromagnet, so that magnetic beads in the tube can be quickly adsorbed to the bottom of the collection tube within a few minutes, and enrichment can be effectively completed at a high speed. The electromagnet can change the rotation direction of the magnetic beads in the liquid of the collecting pipe and is controlled by the alternating current switch, so that the magnetic beads can be uniformly mixed in the solution, and the electromagnet plays a role of magnet when being communicated with the direct current switch and is used for attaching the magnetic beads. If the concentration of the original magnetic beads in the collecting tube is 10mg/ml, collecting supernatant after enriching for different time, detecting the concentration of the supernatant by using a light absorption photometer, wherein the lower the concentration of the supernatant is, the better the adsorption effect is. The concentration of the supernatant enriched at various times and detected by absorbance photometer is shown in table 2.
TABLE 2
| Enrichment time | For 1 minute | 3 minutes | For 5 minutes |
| Concentration of supernatant | 4ng/ml | 0ng/ml | 0ng/ml |
The result shows that the replaceable magnetic rack prepared by the application can complete enrichment within 3 minutes, and the enrichment rate can reach 100 percent.
The two brackets 2 connecting the upper support surface 1 and the base 3 can be formed to be folded outwards after being folded inwards from top to bottom, and the brackets have elasticity of inward compression. The replaceable magnetic rack can be suitable for centrifuge tubes or collecting tubes with different thicknesses, and the centrifuge tubes or the collecting tubes are clamped by inward elasticity of the support, so that the centrifuge tubes or the collecting tubes cannot be separated from the magnetic rack. Meanwhile, the magnetic rack is lighter and easy to grasp.
The shape of the base can be square, round or other shapes; four through holes are symmetrically formed in the upper surface of the base, and the magnetic force of the magnetic field penetrates through the base to effectively act on the magnetic beads in the centrifuge tube or the collecting tube, so that the phenomenon that the base blocks a part of magnetic force is avoided. When the magnetic rack is square, the four sides of the magnetic rack are provided with the inward concave arc-shaped 6, so that the magnetic rack is attractive in appearance, the manufacturing cost is saved, and the enrichment effect is effectively improved. In order to stably place the magnetic frame, the bottom surface of the base is provided with friction pads, and the friction pads can be symmetrically provided with three or four.
As shown in fig. 2, when the upper supporting surface 1 is square, the sharp four corners of the square can be arc-shaped or short-side, which is beautiful and safe to use. The square four sides are provided with the bulges 7 on the two adjacent outer edges, and the other two adjacent edges are provided with the sockets 8 which are matched with the bulges, so that a plurality of different magnetic frames can be combined for use, the number of the magnetic frames can be randomly spliced, the use number can be changed, and the magnetic frames are convenient to carry. Wherein reference numeral 9 of the gray band shown in fig. 2 is used to distinguish the interface.
The magnetic frame prepared by the application can effectively improve the effect of adsorbing pathogens by a magnetic bead method, for example, the efficiency of adsorbing magnetic beads can reach 95%. The microorganism enrichment experiment has good enrichment effect, such as 10-fold ratio gradient dilution of Escherichia coli solution, and taking diluted sample 1 (dilution gradient is 10 -5 ) With sample 2 (dilution gradient 10 -6 ) The magnetic bead method is adopted to adsorb escherichia coli, the magnetic rack is utilized to perform enrichment reaction, the enriched supernatant and the magnetic bead enrichment precipitate are taken to perform colony culture, and the total number of the obtained colonies is shown in table 3.
TABLE 3 Table 3
| Sample numbering | Dilution gradient | Supernatant (cfu/mL) | Magnetic bead enrichment sediment (cfu/mL) |
| 1 | 10 -5 | 19 | 320 |
| 2 | 10 -6 | 0 | 52 |
The result shows that the magnetic rack prepared by the application has good enrichment effect.
Example 2
In this embodiment, based on embodiment 1, the following detachable settings of the neodymium-iron-boron magnet may be adopted: the bottom of base is equipped with the cavity, and the lower extreme of cavity is sealed with the base lid, and sealed structure can adopt: an internal thread is arranged at the port of the lower end of the cavity, an external thread is arranged at the outer edge of the base cover, and the neodymium-iron-boron magnet is arranged in the cavity through the thread. The sealed structure can also adopt: the base cover is buckled with the cavity, the base cover is embedded in the cavity, and the base cover is provided with a small opening or a small groove which can be used for being unscrewed in a rotating mode, so that the base cover is arranged to ensure that the bottom surface is flat, and the base cover can be conveniently taken down.
The magnetic frame prepared by the application can effectively improve the effect of adsorbing pathogens by a magnetic bead method, for example, the efficiency of adsorbing magnetic beads can reach 96%. The microorganism enrichment experiment has good enrichment effect, such as adsorption of Staphylococcus aureus by magnetic bead method, 10-fold gradient dilution of Staphylococcus aureus solution, and dilution of sample 1 (dilution gradient of 10) -3 ) Sample 2 (dilution gradient 10 -4 ) Sample 3 (dilution gradient 10 -5 ) The magnetic bead method is adopted to adsorb staphylococcus aureus, the magnetic rack of the embodiment is utilized to carry out enrichment reaction, the supernatant after enrichment and the magnetic bead enrichment sediment are respectively taken to carry out colony culture, and the total number of the obtained colonies is shown in the table 4, wherein the total number is generally more than 300 and is indispensible.
TABLE 4 Table 4
| Sample numbering | Dilution gradient | Supernatant (cfu/mL) | Magnetic bead enrichment sediment (cfu/mL) |
| 1 | 10 -3 | Multiple incapacitation of meter | Multiple incapacitation of meter |
| 2 | 10 -4 | 2 | Multiple incapacitation of meter |
| 3 | 10 -5 | 0 | 36 |
The result shows that the magnetic rack prepared by the application has good enrichment effect.
The enrichment effect of the magnetic rack is observed by a microscope, as shown in fig. 3, wherein in fig. 3, A is the color of escherichia coli merland, B is a blank captured magnetic bead observation chart under the microscope, C is a captured magnetic bead observation chart under the microscope after the captured magnetic bead is captured for 3 minutes by the magnetic rack prepared by the application, and the result also shows that the magnetic rack prepared by the application has good enrichment effect, can effectively avoid the loss of the magnetic bead along with liquid, and also effectively avoid the occurrence of false negative.
The above description is only a preferred embodiment of the present application, and is not intended to limit the application in any way, and any person skilled in the art may make modifications or alterations to the disclosed technical content to the equivalent embodiments. However, any simple modification, equivalent variation and variation of the above embodiments according to the technical substance of the present application still fall within the protection scope of the technical solution of the present application.
Claims (8)
1. The replaceable magnetic rack for enriching pathogens by a magnetic bead method is characterized by comprising an upper supporting surface and a base, wherein the middle of the upper supporting surface is connected by two symmetrical brackets, an opening is formed in the center of the upper supporting surface, and a small hole is formed in the center of the upper surface of the base, which is opposite to the opening; a neodymium-iron-boron magnet is detachably arranged at the bottom of the base below the small hole, and is obliquely arranged; each support is folded inwards and outwards after being folded inwards, and the support has elasticity of inwards compression;
the shape of the base is triangle, square, regular polygon or round.
2. The replaceable magnetic frame of claim 1, wherein a cavity is formed in the bottom of the base, a groove is formed in the inner side wall of the cavity in a vertically upward direction, a clamping groove is formed in the upper end of the groove in a transverse direction, the size of the neodymium-iron-boron magnet is matched with that of the cavity, and a card clamped with the groove and the clamping groove is arranged on the neodymium-iron-boron magnet.
3. The replaceable magnetic frame of claim 1, wherein a cavity is formed in the bottom of the base, the neodymium-iron-boron magnet is disposed in the cavity, and a base cover is disposed at the lower end of the cavity.
4. The replaceable magnetic frame of claim 1, wherein the oblique arrangement is an angle between the neodymium-iron-boron magnet and the plane of the base of between 0 ° and 30 °.
5. The replaceable magnetic frame of claim 1, wherein the neodymium-iron-boron magnet is replaceable with an electromagnet, and the base is provided with a direct current switch and an alternating current switch for controlling the electromagnet.
6. The replaceable magnetic rack of claim 1, wherein the upper surface of the base is symmetrically provided with three or four through holes.
7. The replaceable magnetic frame of claim 1, wherein the bottom surface of the base is provided with a friction pad.
8. The replaceable magnetic frame of any of claims 1-7, wherein the upper support surface is square, protrusions are provided on two adjacent outer edges, and sockets adapted to the protrusions are provided on two other adjacent edges.
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Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3102920U (en) * | 2004-01-21 | 2004-07-22 | 洋一 広瀬 | Magnet holder and remover |
| CN201384983Y (en) * | 2009-04-10 | 2010-01-20 | 成都思洛生物技术股份有限公司 | Combined type magnetic separating shelf |
| JP2010081915A (en) * | 2008-10-02 | 2010-04-15 | Hitachi Metals Ltd | Cell recovery magnetic stand and cell recovery kit |
| CN205095460U (en) * | 2015-05-13 | 2016-03-23 | 上海快灵生物科技有限公司 | Magnetic bead reagent magnetization unit |
| CN105665132A (en) * | 2014-11-20 | 2016-06-15 | 瀚吉康生物科技（北京）有限公司 | Combined type magnetic frame |
| KR200481282Y1 (en) * | 2015-11-18 | 2016-09-07 | 주식회사 셀레믹스 | Stand for separating magnetic particles |
| CN205603599U (en) * | 2016-05-04 | 2016-09-28 | 浙江道尔生物科技有限公司 | Magnetic shelf with elastic bulge |
| CN205740995U (en) * | 2016-06-01 | 2016-11-30 | 杭州沃森生物技术有限公司 | A kind of universal type replaceable nucleic acid extraction magnetic frame |
| CN206736280U (en) * | 2017-05-25 | 2017-12-12 | 杭州富集生物科技有限公司 | A kind of replaceable magnetic frame for paramagnetic particle method enrichment pathogen |
-
2017
- 2017-05-25 CN CN201710375975.6A patent/CN107012198B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3102920U (en) * | 2004-01-21 | 2004-07-22 | 洋一 広瀬 | Magnet holder and remover |
| JP2010081915A (en) * | 2008-10-02 | 2010-04-15 | Hitachi Metals Ltd | Cell recovery magnetic stand and cell recovery kit |
| CN201384983Y (en) * | 2009-04-10 | 2010-01-20 | 成都思洛生物技术股份有限公司 | Combined type magnetic separating shelf |
| CN105665132A (en) * | 2014-11-20 | 2016-06-15 | 瀚吉康生物科技（北京）有限公司 | Combined type magnetic frame |
| CN205095460U (en) * | 2015-05-13 | 2016-03-23 | 上海快灵生物科技有限公司 | Magnetic bead reagent magnetization unit |
| KR200481282Y1 (en) * | 2015-11-18 | 2016-09-07 | 주식회사 셀레믹스 | Stand for separating magnetic particles |
| CN205603599U (en) * | 2016-05-04 | 2016-09-28 | 浙江道尔生物科技有限公司 | Magnetic shelf with elastic bulge |
| CN205740995U (en) * | 2016-06-01 | 2016-11-30 | 杭州沃森生物技术有限公司 | A kind of universal type replaceable nucleic acid extraction magnetic frame |
| CN206736280U (en) * | 2017-05-25 | 2017-12-12 | 杭州富集生物科技有限公司 | A kind of replaceable magnetic frame for paramagnetic particle method enrichment pathogen |
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