CN209039478U - A kind of high efficiency large volume bottom magnetic StrongmenGroup acquisition means - Google Patents
A kind of high efficiency large volume bottom magnetic StrongmenGroup acquisition means Download PDFInfo
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- CN209039478U CN209039478U CN201821231800.4U CN201821231800U CN209039478U CN 209039478 U CN209039478 U CN 209039478U CN 201821231800 U CN201821231800 U CN 201821231800U CN 209039478 U CN209039478 U CN 209039478U
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Abstract
The utility model provides a kind of high efficiency large volume bottom magnetic StrongmenGroup acquisition means, including frame, feeder, outlet cell spray head, sampling cup and bottom enrichment unit frame, gas sample is input in outlet cell spray head by feeder by appendix, outlet cell spray head, sampling cup and bottom enrichment unit frame are arranged on frame, the lower section of outlet cell spray head is removably arranged in sampling cup, the bottom of sampling cup is located on the enrichment unit frame of bottom and is in plane-plane contact, is equipped with magnetic devices at bottom enrichment unit frame and the contact surface of sampling cup bottom.The beneficial effects of the utility model are: the utility model shortens in such a way that bottom magnetic StrongmenGroup collects liquid phase Magnetic bead sample, it is effectively fixed with acquisition unit bottom surface to acquire screw thread or buckle, the most short operating distance of magnet and magnetic bead is ensured, magnetic force contact surface is expanded, the efficiency of enrichment magnetic bead is improved and shortens enrichment time.
Description
Technical field
The utility model relates to a kind of bioaerosols to acquire enrichment field, substantially more specifically to a kind of high efficiency
Product bottom magnetic StrongmenGroup acquisition means.
Background technique
Bio-capturer aerosol acquisition enriching instrument, which belongs to, integrates the real-time enriched biological of liquid phase magnetic bead by gas-liquid mixed
The sampling instrument of particulate, rotary jet flows into promoting the circulation of qi liquid mixed collection bioaerosol particle apparatus, and (i.e. liquid is adopted
Sample cup, hereinafter referred to as " sampling cup ") the original adoption centrifugation cast that commercialization air sampler generallys use on the market at present
Sampling Design, note: the gas-liquid mixed sampling cup that commercialization air microorganism sampler is related on the market at present is generally straight barrel type
(such as SKC BIO-Sampler, domestic AGI serial air microorganism sampler etc.) or similar to centrifuge tube or test tube shape
Narrow base tapered structure (such as Coriolis air sampler).In practical study, it has been found that bioaerosol acquisition enrichment
The gas-liquid impactor rotation mixing principle gas-liquid mixed efficiency that instrument uses is designed by advanced optimizing gas-liquid mixed structure and shape,
It may be implemented to be obviously improved original sampling cup gas-liquid mixed and bioaerosol collecting efficiency, and can more steadily acquire
Capture bioaerosol.In fact, air-flow agitation mixes situation during determining aerosol collection due to sampling cup-shaped, and
The effective mixed process of gas-liquid is exactly the committed step that particulate acquisition enters liquid phase sample, the common sampling of past commercialization
Though the taper or straight barrel type structure of cup enable to top half liquid in cup sufficiently to stir, the liquid of bottom can not be abundant
Agitation, while the consistent cup-shaped design in mouth bottom is unfavorable for rotating liquid to effective package of air-flow, so that portion gas sample is not
Liquid phase is just had left through gas-liquid mixed, it is undesirable into liquid phase efficiency that these reasons result in aerosol collection.On the other hand, exist
When the liquid phase enrichment with magnetic bead stage collects measuring samples, being limited to the sampling old-fashioned sampling cup of cup structure can only be adsorbed using side wall, magnetic
The gravity of pearl itself affects magnet adsorption effect, causes the magnetic force enrichment that adsorption time is long, and collection liquid cannot sufficiently topple over
The drawback of liquid phase sample inconvenience.
Utility model content
The utility model overcomes deficiency in the prior art, provides a kind of high efficiency large volume bottom magnetic StrongmenGroup packaging
It sets.
The purpose of this utility model is achieved by following technical proposals.
A kind of high efficiency large volume bottom magnetic StrongmenGroup acquisition means, including frame, feeder, outlet cell spray head, sampling
Gas sample is input in the outlet cell spray head by cup and bottom enrichment unit frame, the feeder by appendix,
The outlet cell spray head, the sampling cup and the bottom enrichment unit frame are respectively provided on said frame, the sampling cup
The lower section of the outlet cell spray head is removably set, and the bottom of the sampling cup is located on the bottom enrichment unit frame
And be in plane-plane contact, magnetic devices are equipped at the bottom enrichment unit frame and the contact surface of the sampling cup bottom.
Further, the bottom enrichment unit frame include can be realized the pipe support both horizontally and vertically rotated freely, with
The pallet that described pipe support one end is connected, the pallet are equipped with for the fillet of screw or buckle fixed with the sampling cup, institute
State the center point that the fillet of screw is arranged in magnetic devices.
Further, the magnetic devices are tapered, and the top of the taper is down-set.
Further, the magnetic devices are magnet.
Further, the conical top angle is 120-150 °.
Further, the sampling cup includes cup body and bottom of a cup, and the top of the cup body is equipped with opening, the wall of cup of the cup body
It is inclined outwardly setting, the bottom of a cup is tapered and the top of the taper is prominent to the outside of the cup body, the wall of cup and institute
Bottom of a cup intersection is stated equipped with arc section, the bottom of a cup is equipped with and the matched helicitic texture of the fillet of screw.
Further, the top angle of the taper is 120-150 °.
Further, the radius of the circular arc is 3-4mm.
Further, the wall of cup height is 56.5-90.5mm.
Further, it is 9.3-20.4 ° that the wall of cup unilateral side, which extends out angle,.
The utility model has the following beneficial effects:
Bottom enrichment unit frame has good bioaccumulation efficiency within the scope of 120 ° -150 ° of magnet angle, average to be enriched with
Time shortens 40%；Legacy enrichment bracket obviously can see fraction magnetic bead with the liquid flow toppled in experimentation
Out, when causing the loss of biological sample, and being enriched with using bottom enrichment unit frame, do not have during toppling over collection liquid
There is macroscopic magnetic bead leakage, the loss for avoiding sample of high degree guarantees the magnetic bead body being collected into every time
Product is constant；
Gas-liquid mixed sampling cup provided by the utility model has carried out being different from traditional commerce air on shape and structure
The change of microorganism sampler, commercialization air sampler gas-liquid mixed sampling cup is generally straight barrel type or similar on the market at present
Compared by actual test and optimization in centrifuge tube or the narrow base tapered structure of test tube, the utility model, gas-liquid mixed is adopted
Sample cup has been changed to extend out tapered (trapezoidal bottle), while setting tapered for bottom of a cup, increases the volume in cup body gas-liquid mixed area
While, original gas-liquid mixed mode is also changed, liquid in cup is sufficiently stirred, gas-liquid mixed is not only increased
Contact surface, and the liquid level for rising formation after liquid in rotation is more closed to the package effect of gas sampling spray head, gas-liquid mixed effect
Fruit passes through microculture and tests, it was demonstrated that the efficiency for collecting aerosol is remarkably reinforced；
In general, gas flow rate is an important factor in bioaerosol sampling process, is directly related to live receipts
The gas flow collected, and it is related to the sampling time, but existing research is shown, if the gas flow rate received in sampling cup is too
Greatly, it is formed by gas-liquid and gas wall collision is possible to the microorganism biological that can be damaged in aerosol activity, and then reduce sampling cartridge
Set the collecting effect to bioaerosol.The utility model, which makes sampling cup inner wall integrally by designed arc-shaped appearance, to be become more to put down
It is sliding, the collision of bioaerosol and sampling cup inner wall in sampling process can be effectively reduced, is preferably protected biological in aerosol
The activity of ingredient；
Through different structure shape sampling cup, and actual samples experimental verification, novel sampling cup cup body shape is repeated several times
The good height relationships change with rotation spray port brings being obviously improved for gas-liquid mixed efficiency, and the design of optimization is so that novel adopt
Under conditions of the same air-flow of sample cup, gas-liquid mixed collection biomone is more efficient, gas-liquid mixed severity but further drops
It is low, facilitate microbial activity and stablize, also facilitates the Preconcentration in-situ of liquid phase magnetic bead, therefore the utility model is by being based on
The change of the gas-liquid mixed sampling cup result shape of cyclone principle, novel sampling cup take bottom side to adsorb, and embodiment proves acquisition
Efficiency improves 270% more in the past, and has also been obviously shortened the time of liquid phase enrichment with magnetic bead sample；
The utility model shortens in such a way that bottom magnetic StrongmenGroup collects liquid phase Magnetic bead sample, acquires screw thread or buckle and adopts
It is effectively fixed to collect unit bottom surface, has ensured the most short operating distance of magnet and magnetic bead, has expanded magnetic force contact surface, improve enrichment
The efficiency of magnetic bead and shorten enrichment time.
Detailed description of the invention
Fig. 1 is the utility model structure diagram；
Fig. 2 is the structural schematic diagram of bottom enrichment unit frame；
Fig. 3 is the structural schematic diagram of sampling cup；
Fig. 4 is the structure of the different shape sampling cup of embodiment 4；
Fig. 5 is that different shape sampling cup indoor bacteria collection effect compares histogram；
Fig. 6 is different parameters novel sampling cup bacterium collection effect evaluation comparison histogram；
Fig. 7 is testing result figure in embodiment 6；
In figure:
1, frame；2, pipe support；3, outlet cell spray head；4, sampling cup；5, bottom enrichment unit frame；
6, magnetic devices；7, the fillet of screw；8, pallet；9, cup body；10, it is open；11, graduation mark；12, wall of cup；
13, bottom of a cup；14, helicitic texture；15, arc section；16, screw thread.
Specific embodiment
The technical solution of the utility model is further described below by specific embodiment.
A kind of high efficiency large volume bottom magnetic StrongmenGroup as illustrated in fig. 1 and 2 acquisition means, including frame 1, feeder, outlet
Unit spray head 3, sampling cup 4 and bottom enrichment unit frame 5, gas sample is input to outlet by appendix by the feeder
In unit spray head 3, outlet cell spray head 3, sampling cup 4 and bottom enrichment unit frame 5 are arranged on frame 1, and sampling cup 4 is removable
That unloads is arranged in the lower section of outlet cell spray head 3, and the bottom of sampling cup 4 is located on bottom enrichment unit frame 5 and is in plane-plane contact,
Magnetic devices 6 are equipped at bottom enrichment unit frame 5 and the contact surface of 4 bottom of sampling cup；
Bottom enrichment unit frame 5 includes that can be realized the pipe support both horizontally and vertically rotated freely and the pipe support one
The pallet being connected is held, the pallet is equipped with for the fillet of screw 7 or bayonet fixed with the sampling cup bottom, magnetic devices
6 are arranged in the center point of the fillet of screw 7；Magnetic devices 6 are tapered, and the top of the taper is down-set, and magnetic devices 6 are magnetic
Iron, the conical top angle are 120 ° -150 °.
As shown in figure 3, sampling cup 4 includes cup body 1 and bottom of a cup 5, the top of cup body 1 is equipped with opening 2, is equipped with spiral shell at opening 2
Line 8, the wall of cup 4 of cup body 1 are inclined outwardly setting, and it is 9.3 ° -20.4 ° that 4 unilateral side of wall of cup, which extends out angle, it is preferable that is 15 °；Wall of cup 4
Height be 56.5-90.5mm, wall of cup 4 be equipped with graduation mark；Bottom of a cup 5 is tapered and the top of the taper is to the outer of cup body 1
Side is prominent, and the top angle of the taper is 120-150 °, it is preferable that angle is 140 °, and helicitic texture 6 is arranged in the taper
Top below, bottom of a cup 5 is equipped with helicitic texture 6, and the radius of helicitic texture 6 is less than the radius of bottom of a cup 5；Wall of cup 4 and bottom of a cup 5 are had a common boundary
Place is equipped with arc section 7, and the radius of circular arc 7 is 3-4mm, it is preferable that the radius of arc section 7 is 3.8cm.
In use, sampling cup 4 is connected by helicitic texture 14 with bottom enrichment unit frame 5, bottom of a cup 5 and magnetic devices 6 are tight
Contiguity touching carries out absorption work.
Example 1 is enriched with the evaluation and test of bracket enrichment with magnetic bead effect
Enrichment with magnetic bead test is carried out using new and old sampling cup respectively, appropriate collection liquid is added in sampling cup first, then
Three drops magnetic bead (about 150 μ l) of equivalent are separately added into, being sufficiently stirred is dispersed in magnetic bead in collection liquid.Simultaneously by sampling cup
It is put into enrichment bracket, manual time-keeping.In triplicate, record magnetic bead is enriched with completely, and collection liquid restores settling time.
By repeating to test, the average enrichment time that bottom is enriched with bracket is 3m24s, when legacy enrichment bracket is averagely enriched with
Between be 5m40s, enrichment time shortens 40%.
The evaluation and test of the new and old enrichment bracket magnetic bead recovering effect of example 2
Magnetic bead, which is carried out, using new and old sampling cup respectively recycles comparative experiments, after appropriate collection liquid is respectively added in sampling cup,
Equivalent three is added and drips (about 150 μ l) magnetic bead, being sufficiently stirred is enriched with magnetic bead after mixing.It is sufficiently enriched with, receives to magnetic bead
After liquid collecting clarification, rotation enrichment bracket topples over collection liquid, the magnetic bead after being enriched with.
Legacy enrichment bracket obviously can see fraction magnetic bead as the liquid toppled over flows out in experimentation, cause
The loss of biological sample.And bottom is enriched with bracket and is lost now during toppling over collection liquid without there is macroscopic magnetic bead
As the loss for avoiding sample of high degree.Bottom enrichment bracket can be by the complete evacuation of collection liquid and legacy is enriched with bracket
Collection liquid can not all be poured out due to sampling bottle tapered structure itself, this also can not just determine the magnetic being collected into every time
Bead product is constant.
Example 3 tests the change of bottom enrichment frame bottom magnet angle
Bottom sampling support devises 110 °, 120 °, 130 °, 140 °, 150 °, 160 ° of six bottom magnet tilt angles,
And the experiment of enrichment with magnetic bead Time evaluation is carried out to them.
Three repeated experiments are carried out respectively to six kinds of enrichment brackets, the average enrichment time of six kinds of enrichment brackets is corresponding such as
Under:
| Support angle (°) | 110 | 120 | 130 | 140 | 150 | 160 |
| Enrichment time (s) | 368 | 320 | 211 | 204 | 287 | 352 |
Experiments have shown that other than 110 ° and 160 ° of enrichment bracket enrichment time is greater than legacy enrichment bracket, remaining four
The enrichment bracket bioaccumulation efficiency of angle is superior to legacy enrichment bracket.Therefore enrichment bracket in bottom is at 120 ° -150 ° of magnet angle
Within the scope of have good bioaccumulation efficiency.
The evaluation of 4 different shape sampling cup indoor bacteria collection effect of example
Three improvement directions of sampling cup are designed according to primary prospect, and are customized out such as experiment cup type and old sampling in Fig. 4
Cup carries out indoor aerosols and acquires comparative experiments.Five laboratories are chosen as aerosol and acquire experimental place, are adopted using four kinds
Sample cup carry out sequence completely random sampling.Wherein, N, o, former pipe are common commercial sampling cup ideographs, and W is the utility model
Middle novel sampling cup ideograph, the collection liquid coated plate that will be collected into carry out bacterium colony counting to LB plate after 48h.
Bacterium colony count results are passed through into formulaIt is converted into environmental bacteria concentration C
(unit CFU/m3), experiment is repeated several times, each each sampling cup acquisition situation of place bacterial concentration average value drafting is taken to compare column
Figure, such as Fig. 5.
The sampling cup aerosol collection effect designed in experiment discovery the utility model is substantially better than its excess-three kind, therefore really
The improvement direction of novel sampling cup is determined.
The evaluation of 5 different parameters novel sampling cup bacterium collection effect of example
Change its angle for the sampling cup designed in the utility model and height designs other four kinds of sampling cups, chooses
The environmental aerosols of four fixed laboratory carry out sequence completely randoms acquire experiment, and by collection liquid coated plate, carry out bacterium after 48h
Fall counting.Each laboratory bacterial mean concentration is counted using same procedure, each sampling cup acquisition situation is drawn and compares histogram,
Such as Fig. 6.
Experiment discovery extends out 9.3 ° to 20.4 ° of angle in unilateral side, height 56.5-90.5mm novel sampling cup within the scope of this
It can realize the acquisition to surrounding air bacterium, wherein unilateral side, which extends out 15 ° of this sampling cups, collecting efficiency the most good.
The new and old sampling cup environmental bacteria of embodiment 6 acquires situation evaluation (real-time PCR method)
It selects two chicken farms to be acquired efficiency rating experiment to new legacy sampling pipe, is placed in each chicken house new and old
Collected aerosol sample extraction nucleic acid is carried out real time PCR detection, the following Fig. 7 of testing result by two kinds of samplers
It is shown.
Repetition experimental result is similar to the above results, experiments have shown that the sample of the sampling cup acquisition designed in the utility model
It is lower than old sampling cup sample through PCR detection CT value, it was demonstrated that the concentration of specimens of novel sampling cup acquisition is higher.
By analyzing the result that experiment is repeated several times, novel sampling cup adopts the collecting efficiency of environmental bacteria than old
Sample cup improves 270%, substantially increases the collecting efficiency of environmental aerosols.
One embodiment of the utility model is described in detail above, but the content is only the utility model
Preferred embodiment should not be considered as limiting the scope of the present invention.It is all to be made according to application scope of the utility model
All the changes and improvements etc., should still belong within the patent covering scope of the utility model.
Claims (10)
1. a kind of high efficiency large volume bottom magnetic StrongmenGroup acquisition means, it is characterised in that: including frame, feeder, outlet cell
Gas sample is input to the outlet list by appendix by spray head, sampling cup and bottom enrichment unit frame, the feeder
In first spray head, the outlet cell spray head, the sampling cup and the bottom enrichment unit frame are respectively provided on said frame, institute
The lower section that the outlet cell spray head is removably arranged in sampling cup is stated, the bottom of the sampling cup is located at bottom enrichment
It is directly contacted on unit frame and in face face, magnetic force is equipped at the contact surface of the bottom enrichment unit frame and the sampling cup bottom
Device.
2. high efficiency large volume bottom magnetic StrongmenGroup according to claim 1 acquisition means, it is characterised in that: the bottom enrichment
Unit frame includes the pallet that can be realized the pipe support both horizontally and vertically rotated freely, be connected with described pipe support one end, institute
It states pallet to be equipped with for the fillet of screw or buckle fixed with the sampling cup, the fillet of screw is arranged in the magnetic means
The center point.
3. high efficiency large volume bottom magnetic StrongmenGroup according to claim 2 acquisition means, it is characterised in that: the magnetic means
Tapered, the top of the taper is down-set.
4. high efficiency large volume bottom magnetic StrongmenGroup according to claim 1 acquisition means, it is characterised in that: the magnetic means
For magnet.
5. high efficiency large volume bottom magnetic StrongmenGroup according to claim 3 acquisition means, it is characterised in that: the top of the taper
Portion's angle is 120-150 °.
6. high efficiency large volume bottom magnetic StrongmenGroup according to claim 2 acquisition means, it is characterised in that: the sampling cup packet
Cup body and bottom of a cup are included, the top of the cup body is equipped with opening, and the wall of cup of the cup body is inclined outwardly setting, and the bottom of a cup is tapered
And the top of the taper is prominent to the outside of the cup body, the wall of cup and the bottom of a cup intersection are equipped with arc section, described
Bottom of a cup is equipped with and the matched helicitic texture of the fillet of screw.
7. high efficiency large volume bottom magnetic StrongmenGroup according to claim 6 acquisition means, it is characterised in that: the top of the taper
Portion's angle is 120-150 °.
8. high efficiency large volume bottom magnetic StrongmenGroup according to claim 6 acquisition means, it is characterised in that: the half of the circular arc
Diameter is 3-4mm.
9. high efficiency large volume bottom magnetic StrongmenGroup according to claim 6 acquisition means, it is characterised in that: the wall of cup height
For 56.5-90.5mm.
10. high efficiency large volume bottom magnetic StrongmenGroup according to claim 6 acquisition means, it is characterised in that: the wall of cup list
It is 9.3-20.4 ° that side, which extends out angle,.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821231800.4U CN209039478U (en) | 2018-08-01 | 2018-08-01 | A kind of high efficiency large volume bottom magnetic StrongmenGroup acquisition means |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821231800.4U CN209039478U (en) | 2018-08-01 | 2018-08-01 | A kind of high efficiency large volume bottom magnetic StrongmenGroup acquisition means |
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| Publication Number | Publication Date |
|---|---|
| CN209039478U true CN209039478U (en) | 2019-06-28 |
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|---|---|---|---|
| CN201821231800.4U Active CN209039478U (en) | 2018-08-01 | 2018-08-01 | A kind of high efficiency large volume bottom magnetic StrongmenGroup acquisition means |
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- 2018-08-01 CN CN201821231800.4U patent/CN209039478U/en active Active
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