CN109251849A - A kind of device of high efficient gas and liquid mixed collection bioaerosol - Google Patents
A kind of device of high efficient gas and liquid mixed collection bioaerosol Download PDFInfo
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- CN109251849A CN109251849A CN201810874895.XA CN201810874895A CN109251849A CN 109251849 A CN109251849 A CN 109251849A CN 201810874895 A CN201810874895 A CN 201810874895A CN 109251849 A CN109251849 A CN 109251849A
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- cup
- bioaerosol
- liquid mixed
- high efficient
- efficient gas
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
Abstract
The present invention provides a kind of device of high efficient gas and liquid mixed collection bioaerosol, including cup body and bottom of a cup, the top of cup body is equipped with opening, the wall of cup of cup body is inclined outwardly setting, in mouth handle big structure, bottom of a cup is tapered and the top of taper is prominent to the outside of cup body, and wall of cup and bottom of a cup intersection are equipped with arc section.The beneficial effects of the present invention are: the change of novel sampling cup cup body structure shape brings the change of bioaerosol gas-liquid mixed mode, bioaerosol collecting efficiency improves 270% more in the past, novel sampling cup takes bottom side magnetic-adsorption, hence it is evident that shortens liquid phase Magnetic bead sample enrichment time.
Description
Technical field
The present invention relates to bioaerosol Enrichment fields, raw more specifically to a kind of high efficient gas and liquid mixed collection
The device of object aerosol.
Background technique
Bio-capturer aerosol acquisition enriching instrument, which belongs to, integrates the real-time enriched biological of liquid phase magnetic bead by gas-liquid mixed
The sampling instrument of particulate, rotary jet flows into promoting the circulation of qi liquid mixed collection bioaerosol particle apparatus, and (i.e. liquid is adopted
Sample cup, hereinafter referred to as " sampling cup ") the original adoption centrifugation cast that commercialization air sampler generallys use on the market at present
Sampling Design, note: the gas-liquid mixed sampling cup that commercialization air microorganism sampler is related on the market at present is generally straight barrel type
(such as SKC BIO-Sampler, domestic AGI serial air microorganism sampler etc.) or similar to centrifuge tube or test tube shape
Narrow base tapered structure (such as Coriolis air sampler).In practical study, it has been found that bioaerosol acquisition enrichment
The gas-liquid impactor rotation mixing principle gas-liquid mixed efficiency that instrument uses is designed by advanced optimizing gas-liquid mixed structure and shape,
It may be implemented to be obviously improved original sampling cup gas-liquid mixed and bioaerosol collecting efficiency, and can more steadily acquire
Capture bioaerosol.In fact, air-flow agitation mixes situation during determining aerosol collection due to sampling cup-shaped, and
The effective mixed process of gas-liquid is exactly the committed step that particulate acquisition enters liquid phase sample, the common sampling of past commercialization
Though the taper or straight barrel type structure of cup enable to top half liquid in cup sufficiently to stir, the liquid of bottom can not be abundant
Agitation, while the consistent cup-shaped design in mouth bottom is unfavorable for rotating liquid to effective package of air-flow, so that portion gas sample is not
Liquid phase is just had left through gas-liquid mixed, it is undesirable into liquid phase efficiency that these reasons result in aerosol collection.On the other hand, exist
When the liquid phase enrichment with magnetic bead stage collects measuring samples, being limited to the sampling old-fashioned sampling cup of cup structure can only be adsorbed using side wall, magnetic
The gravity of pearl itself affects magnet adsorption effect, causes the magnetic force enrichment that adsorption time is long, and collection liquid cannot sufficiently topple over
The drawback of liquid phase sample inconvenience.
Summary of the invention
The present invention overcomes deficiencies in the prior art, provide a kind of dress of high efficient gas and liquid mixed collection bioaerosol
It sets.
The purpose of the present invention is achieved by following technical proposals.
A kind of device of high efficient gas and liquid mixed collection bioaerosol, including cup body and bottom of a cup are set at the top of the cup body
There is opening, the wall of cup of the cup body is inclined outwardly setting, and in the big trapezoidal cup-shaped of mouth handle, the bottom of a cup is tapered and described
The top of taper is prominent to the outside of the cup body, and the wall of cup and the bottom of a cup intersection are equipped with arc section.
Further, the bottom of a cup is equipped with screw thread or buckle structure, coincide convenient for magnet and the effective of bottom.
Further, the radius of the screw thread or buckle structure is less than the radius of the bottom of a cup.
Further, the screw thread or buckle structure are arranged in below the top of the taper.
Further, the top angle of the taper is 120-150 °.
Further, the radius of the circular arc is 3-4mm.
Further, the height of the wall of cup is 56.5-90.5mm.
Further, it is 9.3-20.4 ° that the wall of cup unilateral side, which extends out angle,.
Further, the opening is equipped with screw thread.
Further, the wall of cup is equipped with graduation mark.
The invention has the benefit that
Gas-liquid mixed sampling cup provided by the invention has carried out being different from the micro- life of traditional commerce air on shape and structure
The change of object sampler, at present on the market commercialization air sampler gas-liquid mixed sampling cup be generally straight barrel type or be similar to from
The narrow base tapered structure of heart pipe or test tube, the present invention are compared by actual test and optimization, and gas-liquid mixed sampling cup is changed to
It extends out tapered (trapezoidal bottle), while setting tapered for bottom of a cup, while increasing the volume in cup body gas-liquid mixed area,
Original gas-liquid mixed mode is changed, liquid in cup is sufficiently stirred, not only increases gas-liquid mixed contact surface, and
And the liquid level for rising formation after liquid in rotation is more closed to the package effect of gas sampling spray head, gas-liquid mixed effect passes through micro- life
Object culture experiment, it was demonstrated that the efficiency for collecting aerosol is remarkably reinforced；
In general, gas flow rate is an important factor in bioaerosol sampling process, is directly related to live receipts
The gas flow collected, and it is related to the sampling time, but existing research is shown, if the gas flow rate received in sampling cup is too
Greatly, it is formed by gas-liquid and gas wall collision is possible to the microorganism biological that can be damaged in aerosol activity, and then reduce sampling cartridge
Set the collecting effect to bioaerosol.The present invention, which makes sampling cup inner wall integrally by designed arc-shaped appearance, becomes more smooth, can
The collision of bioaerosol and sampling cup inner wall in sampling process is effectively reduced, biotic component in aerosol is preferably protected
Activity.
Through different structure shape sampling cup, and actual samples experimental verification, novel sampling cup cup body shape is repeated several times
The good height relationships change with rotation spray port brings being obviously improved for gas-liquid mixed efficiency, and the design of optimization is so that novel adopt
Under conditions of the same air-flow of sample cup, gas-liquid mixed collection biomone is more efficient, gas-liquid mixed severity but further drops
It is low, facilitate microbial activity and stablize, also facilitates the Preconcentration in-situ of liquid phase magnetic bead, therefore the present invention is by being based on cyclone
The change of the gas-liquid mixed sampling cup result shape of principle, novel sampling cup take bottom side to adsorb, and embodiment proves collecting efficiency
270% is improved more in the past, and has also been obviously shortened the time of liquid phase enrichment with magnetic bead sample.
Detailed description of the invention
Fig. 1 is schematic structural view of the invention；
Fig. 2 is the structure of the different shape sampling cup of embodiment 1；
Fig. 3 is that different shape sampling cup indoor bacteria collection effect compares histogram；
Fig. 4 is different parameters novel sampling cup bacterium collection effect evaluation comparison histogram；
Fig. 5 is testing result figure in embodiment 4；
In figure:
1, cup body；2, it is open；3, graduation mark；4, wall of cup；5, bottom of a cup；6, helicitic texture；
7, arc section；8, screw thread.
Specific embodiment
Below by specific embodiment, further description of the technical solution of the present invention.
A kind of device of high efficient gas and liquid mixed collection bioaerosol, including cup body 1 and bottom of a cup 5, the top of cup body 1 are equipped with
Opening 2 is equipped with screw thread 8 at opening 2, and the wall of cup 4 of cup body 1 is inclined outwardly setting, 4 unilateral side of wall of cup extend out angle for 9.3 °-
20.4 °, it is preferable that be 15 °；The height of wall of cup 4 is 56.5-90.5mm, and wall of cup 4 is equipped with graduation mark；Bottom of a cup 5 is tapered and institute
The top for stating taper is prominent to the outside of cup body 1, and the top angle of the taper is 120-150 °, it is preferable that and angle is 140 °,
Helicitic texture 6 is arranged in below the top of the taper, and bottom of a cup 5 is equipped with helicitic texture 6, and the radius of helicitic texture 6 is less than bottom of a cup 5
Radius；Wall of cup 4 and 5 intersection of bottom of a cup are equipped with arc section 7, and the radius of circular arc 7 is 3-4mm, it is preferable that the radius of arc section 7
For 3.8cm.
In use, being connected by helicitic texture 6 with the enrichment unit frame for being equipped with magnet in bottom, bottom of a cup 5 and magnet are close
Contact carries out absorption work.
The evaluation of 1 different shape sampling cup indoor bacteria collection effect of example
Three improvement directions of sampling cup are designed according to primary prospect, and are customized out such as experiment cup type and old sampling in Fig. 2
Cup carries out indoor aerosols and acquires comparative experiments.Five laboratories are chosen as aerosol and acquire experimental place, are adopted using four kinds
Sample cup carry out sequence completely random sampling.Wherein, N, o, former pipe are common commercial sampling cup ideographs, and W is new in the present invention
Type sampling cup ideograph, the collection liquid coated plate that will be collected into carry out bacterium colony counting to LB plate after 48h.
Bacterium colony count results are passed through into formulaIt is converted into environmental bacteria concentration C
(unit CFU/m3), experiment is repeated several times, each each sampling cup acquisition situation of place bacterial concentration average value drafting is taken to compare column
Figure, such as Fig. 3.
The sampling cup aerosol collection effect designed in the experiment discovery present invention is substantially better than its excess-three kind, it is thus determined that
The improvement direction of novel sampling cup.
The evaluation of 2 different parameters novel sampling cup bacterium collection effect of example
Change its angle for the sampling cup designed in the present invention and height designs other four kinds of sampling cups, chooses four
The environmental aerosols of fixed laboratory carry out sequence completely random acquire experiment, and by collection liquid coated plate, carry out bacterium colony meter after 48h
Number.Each laboratory bacterial mean concentration is counted using same procedure, each sampling cup acquisition situation is drawn and compares histogram, such as scheme
4。
Experiment discovery extends out 9.3 ° to 20.4 ° of angle in unilateral side, height 56.5-90.5mm novel sampling cup within the scope of this
It can realize the acquisition to surrounding air bacterium, wherein unilateral side, which extends out 15 ° of this sampling cups, collecting efficiency the most good.
The new and old sampling cup environmental bacteria of embodiment 3 acquires situation evaluation (real-time PCR method)
It selects two chicken farms to be acquired efficiency rating experiment to new legacy sampling pipe, is placed in each chicken house new and old
Two kinds of samplers examine the Real time PCR that collected aerosol sample extraction nucleic acid carries out bacterial 16 S house-keeping gene
It surveys, testing result amplification curve and CT value are illustrated in fig. 5 shown below.
Repetition experimental result is similar to the above results, experiments have shown that the sample warp of the sampling cup acquisition designed in the present invention
The CT value of 16SDNA is significantly lower than control sampling cup sample in the novel sampling cup of Realtime-PCR detection double sampling,
It proves higher using the bacterial micro-organism aerosol concentration of specimens of novel sampling cup acquisition.
By analyzing the result that experiment is repeated several times, novel sampling cup adopts the collecting efficiency of environmental bacteria than old
Sample cup improves 270%, substantially increases the collecting efficiency of environmental aerosols.
Example 4 tests the change of angle at the top of novel sampling cup-and-cone type
The sampling cup of 110 °, 120 °, 130 °, 140 °, 150 °, 160 ° six kinds of tapered angles is designed and produced, and to them
The experiment of enrichment with magnetic bead Time evaluation is carried out.
Six kinds of sampling cups are carried out respectively to repeat enrichment with magnetic bead experiment three times, the average enrichment time of six kinds of sampling cups is corresponding
It is as follows:
| Tapered angle (°) | 110 | 120 | 130 | 140 | 150 | 160 |
| Enrichment time (s) | 368 | 320 | 211 | 204 | 287 | 352 |
Experiments have shown that other than 110 ° and 160 ° of sampling cup enrichment time is greater than legacy sampling cup, remaining four angle
Sampling cup bioaccumulation efficiency be superior to legacy sampling cup.Therefore novel sampling cup is within the scope of tapered angle is 120 ° -150 °
There is good bioaccumulation efficiency.
Five embodiments of the invention are described in detail above, but the content is only preferable implementation of the invention
Example, should not be considered as limiting the scope of the invention.It is all according to all the changes and improvements made by the present patent application range
Deng should still belong to patent covering scope of the invention.
Claims (10)
1. a kind of device of high efficient gas and liquid mixed collection bioaerosol, it is characterised in that: including cup body and bottom of a cup, the cup body
Top be equipped with opening, the wall of cup of the cup body is inclined outwardly setting, in the big cup-like structure of mouth handle, the bottom of a cup it is tapered and
The top of the taper is prominent to the outside of the cup body, and the wall of cup and the bottom of a cup intersection are equipped with arc section.
2. the device of high efficient gas and liquid mixed collection bioaerosol according to claim 1, it is characterised in that: the bottom of a cup
Equipped with screw thread or buckle structure.
3. the device of high efficient gas and liquid mixed collection bioaerosol according to claim 2, it is characterised in that: the screw thread
The radius of structure is less than the radius of the bottom of a cup.
4. the device of high efficient gas and liquid mixed collection bioaerosol according to claim 3, it is characterised in that: the screw thread
Structure setting is below the top of the taper.
5. the device of high efficient gas and liquid mixed collection bioaerosol according to claim 4, it is characterised in that: the taper
Top angle be 120-150 °.
6. the device of high efficient gas and liquid mixed collection bioaerosol according to claim 1, it is characterised in that: the circular arc
Radius be 3-4mm.
7. the device of high efficient gas and liquid mixed collection bioaerosol according to claim 1, it is characterised in that: the wall of cup
Height be 56.5-90.5mm.
8. the device of high efficient gas and liquid mixed collection bioaerosol according to claim 1, it is characterised in that: the wall of cup
It is 9.3-20.4 ° that unilateral side, which extends out angle,.
9. the device of high efficient gas and liquid mixed collection bioaerosol described in -8 according to claim 1, it is characterised in that: described to open
Screw thread or buckle structure are equipped at mouthful.
10. the device of high efficient gas and liquid mixed collection bioaerosol according to claim 9, it is characterised in that: the cup
Wall is equipped with graduation mark.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810874895.XA CN109251849A (en) | 2018-08-01 | 2018-08-01 | A kind of device of high efficient gas and liquid mixed collection bioaerosol |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810874895.XA CN109251849A (en) | 2018-08-01 | 2018-08-01 | A kind of device of high efficient gas and liquid mixed collection bioaerosol |
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| CN109251849A true CN109251849A (en) | 2019-01-22 |
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| CN201810874895.XA Pending CN109251849A (en) | 2018-08-01 | 2018-08-01 | A kind of device of high efficient gas and liquid mixed collection bioaerosol |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110592179A (en) * | 2019-09-16 | 2019-12-20 | 中国科学院微生物研究所 | Biological aerosol collecting method based on rotary liquid cavity |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5954845A (en) * | 1996-04-26 | 1999-09-21 | Skc, Inc. | Apparatus for sampling aerosols |
| RU2397801C2 (en) * | 2008-06-24 | 2010-08-27 | Самсунг Электроникс Ко., Лтд. | Device and method for collection of aerosol particles and their concentration definition |
| CN202688324U (en) * | 2012-03-30 | 2013-01-23 | 中国检验检疫科学研究院 | Bio-aerosol collection gathering device |
| CN206910353U (en) * | 2016-12-07 | 2018-01-23 | 武汉迪艾斯科技有限公司 | Biological specimen collection cup |
| CN208545430U (en) * | 2018-08-01 | 2019-02-26 | 杭州富集生物科技有限公司 | A kind of device of high efficient gas and liquid mixed collection bioaerosol |
-
2018
- 2018-08-01 CN CN201810874895.XA patent/CN109251849A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5954845A (en) * | 1996-04-26 | 1999-09-21 | Skc, Inc. | Apparatus for sampling aerosols |
| RU2397801C2 (en) * | 2008-06-24 | 2010-08-27 | Самсунг Электроникс Ко., Лтд. | Device and method for collection of aerosol particles and their concentration definition |
| CN202688324U (en) * | 2012-03-30 | 2013-01-23 | 中国检验检疫科学研究院 | Bio-aerosol collection gathering device |
| CN206910353U (en) * | 2016-12-07 | 2018-01-23 | 武汉迪艾斯科技有限公司 | Biological specimen collection cup |
| CN208545430U (en) * | 2018-08-01 | 2019-02-26 | 杭州富集生物科技有限公司 | A kind of device of high efficient gas and liquid mixed collection bioaerosol |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110592179A (en) * | 2019-09-16 | 2019-12-20 | 中国科学院微生物研究所 | Biological aerosol collecting method based on rotary liquid cavity |
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