CN106967682A - 一种nk细胞体外扩增方法 - Google Patents
一种nk细胞体外扩增方法 Download PDFInfo
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- CN106967682A CN106967682A CN201710375838.2A CN201710375838A CN106967682A CN 106967682 A CN106967682 A CN 106967682A CN 201710375838 A CN201710375838 A CN 201710375838A CN 106967682 A CN106967682 A CN 106967682A
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- 238000000034 method Methods 0.000 title claims abstract description 27
- 210000000822 natural killer cell Anatomy 0.000 title claims abstract description 26
- 230000010261 cell growth Effects 0.000 title claims abstract description 17
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 70
- 210000004027 cell Anatomy 0.000 claims abstract description 29
- 210000003819 peripheral blood mononuclear cell Anatomy 0.000 claims abstract description 14
- 210000002966 serum Anatomy 0.000 claims abstract description 12
- 238000005119 centrifugation Methods 0.000 claims abstract description 7
- 210000005259 peripheral blood Anatomy 0.000 claims abstract description 7
- 210000004698 lymphocyte Anatomy 0.000 claims abstract description 4
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- 238000002360 preparation method Methods 0.000 claims abstract description 4
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- 238000004458 analytical method Methods 0.000 claims description 5
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- 108010002350 Interleukin-2 Proteins 0.000 claims description 4
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- 102000015696 Interleukins Human genes 0.000 description 1
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0646—Natural killers cells [NK], NKT cells
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/20—Cytokines; Chemokines
- C12N2501/23—Interleukins [IL]
- C12N2501/2302—Interleukin-2 (IL-2)
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/20—Cytokines; Chemokines
- C12N2501/23—Interleukins [IL]
- C12N2501/2312—Interleukin-12 (IL-12)
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/20—Cytokines; Chemokines
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- C12N2501/2315—Interleukin-15 (IL-15)
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/50—Cell markers; Cell surface determinants
- C12N2501/515—CD3, T-cell receptor complex
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/50—Cell markers; Cell surface determinants
- C12N2501/599—Cell markers; Cell surface determinants with CD designations not provided for elsewhere
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Abstract
Description
孵育天数 | 常规组 | 实验组 |
D0 | 0.5×108 | 0.5×108 |
D3 | 3.2×108 | 5.1×108 |
D6 | 6.7×108 | 11.4×108 |
D9 | 14.5×108 | 23.8×108 |
D12 | 30.6×108 | 49.8×108 |
D14 | 68.6×108 | 106.8×108 |
Claims (5)
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CN201710375838.2A CN106967682B (zh) | 2017-05-25 | 2017-05-25 | 一种nk细胞体外扩增方法 |
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CN201710375838.2A CN106967682B (zh) | 2017-05-25 | 2017-05-25 | 一种nk细胞体外扩增方法 |
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CN106967682A true CN106967682A (zh) | 2017-07-21 |
CN106967682B CN106967682B (zh) | 2020-08-07 |
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Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109294985A (zh) * | 2018-10-25 | 2019-02-01 | 江苏普瑞康生物医药科技有限公司 | 一种用于nk细胞体外扩增的培养基体系及nk细胞体外扩增的方法 |
CN109486759A (zh) * | 2019-01-17 | 2019-03-19 | 汇麟生物科技(北京)有限公司 | 一种脐带血nk细胞的培养方法 |
CN109504658A (zh) * | 2019-01-17 | 2019-03-22 | 药鼎(北京)国际细胞医学技术有限公司 | 一种胎盘血nk细胞的培养方法 |
CN109517052A (zh) * | 2018-12-04 | 2019-03-26 | 江苏禄亿生生物科技有限公司 | 一种il-12蛋白突变体及其制备方法和应用、nk细胞培养体系和培养nk细胞的方法 |
CN110628715A (zh) * | 2018-06-21 | 2019-12-31 | 精准生技股份有限公司 | 体外扩增自然杀手细胞及自然杀手t细胞的方法及其医药组成物 |
CN111286487A (zh) * | 2020-01-17 | 2020-06-16 | 南宁摩米生物科技有限公司 | 一种多细胞因子诱导的高纯度nk细胞培养方法 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102994449A (zh) * | 2012-12-13 | 2013-03-27 | 上海柯莱逊生物技术有限公司 | 一种体外扩增nk细胞的方法 |
CN105754942A (zh) * | 2016-05-18 | 2016-07-13 | 天津普瑞赛尔生物科技有限公司 | 一种体外扩增nk细胞的方法及其获得的nk细胞 |
EP3444332A1 (en) * | 2016-02-15 | 2019-02-20 | Dong Hyuk Shin | Medium addition kit for culturing nk cells and nk cell culturing method using kit |
-
2017
- 2017-05-25 CN CN201710375838.2A patent/CN106967682B/zh active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102994449A (zh) * | 2012-12-13 | 2013-03-27 | 上海柯莱逊生物技术有限公司 | 一种体外扩增nk细胞的方法 |
CN103756963A (zh) * | 2012-12-13 | 2014-04-30 | 上海柯莱逊生物技术有限公司 | 一种体外扩增nk细胞的方法 |
EP3444332A1 (en) * | 2016-02-15 | 2019-02-20 | Dong Hyuk Shin | Medium addition kit for culturing nk cells and nk cell culturing method using kit |
CN105754942A (zh) * | 2016-05-18 | 2016-07-13 | 天津普瑞赛尔生物科技有限公司 | 一种体外扩增nk细胞的方法及其获得的nk细胞 |
Non-Patent Citations (2)
Title |
---|
E.TAKAHASHI ET AL.: ""Induction of CD16+ CD56bright NK Cells with Antitumour Cytotoxicity not only fromC D16- CD56bright NK Cells but also from CD16-CD56dim NK Cells"", 《SCANDINAVIAN JOURNAL OF IMMUNOLOGY》 * |
李晓红等: ""在造血干细胞培养基条件下应用培养及条件下应用IL2、12、15扩增高纯度外周血来源的细胞"", 《第11次中国实验血液学会议论文汇编》 * |
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110628715A (zh) * | 2018-06-21 | 2019-12-31 | 精准生技股份有限公司 | 体外扩增自然杀手细胞及自然杀手t细胞的方法及其医药组成物 |
CN110628715B (zh) * | 2018-06-21 | 2022-11-25 | 精准生技股份有限公司 | 体外扩增自然杀手细胞及自然杀手t细胞的方法及其医药组成物 |
CN109294985A (zh) * | 2018-10-25 | 2019-02-01 | 江苏普瑞康生物医药科技有限公司 | 一种用于nk细胞体外扩增的培养基体系及nk细胞体外扩增的方法 |
CN109517052A (zh) * | 2018-12-04 | 2019-03-26 | 江苏禄亿生生物科技有限公司 | 一种il-12蛋白突变体及其制备方法和应用、nk细胞培养体系和培养nk细胞的方法 |
CN109486759A (zh) * | 2019-01-17 | 2019-03-19 | 汇麟生物科技(北京)有限公司 | 一种脐带血nk细胞的培养方法 |
CN109504658A (zh) * | 2019-01-17 | 2019-03-22 | 药鼎(北京)国际细胞医学技术有限公司 | 一种胎盘血nk细胞的培养方法 |
CN111286487A (zh) * | 2020-01-17 | 2020-06-16 | 南宁摩米生物科技有限公司 | 一种多细胞因子诱导的高纯度nk细胞培养方法 |
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