CN106962273B - Embryo egg hatching method for improving production characteristics of hens - Google Patents
Embryo egg hatching method for improving production characteristics of hens Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K45/00—Other aviculture appliances, e.g. devices for determining whether a bird is about to lay
- A01K45/007—Injecting or otherwise treating hatching eggs
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- Life Sciences & Earth Sciences (AREA)
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- Animal Husbandry (AREA)
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- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses an embryo egg hatching method for improving the production characteristics of hens, which belongs to the technical field of agricultural breeding and comprises the following steps: (1) treating for 4-5 days of incubation, (2) selecting female chick embryo eggs, (3) treating for 12-13 days of incubation, and (4) finishing incubation. In the process of hatching the fertilized hatching eggs, the invention reasonably carries out special treatment in different hatching periods, effectively improves the synthetic amount of immunoglobulin in the embryo eggs, improves the development speed and quality of various organs, fully utilizes the original nutrient substances in the fertilized hatching eggs, finally breeds have the advantages of good body quality and strong production characteristics, and have commercial value.
Description
Technical Field
The invention belongs to the technical field of agricultural breeding, and particularly relates to an embryo egg hatching method for improving the production characteristics of hens.
Background
The sex of chickens is closely related to their productivity. In commercial egg production, hens are used as the only production tool, and cocks are eliminated without production capacity. In the production of fertilized eggs, the cocks are only used as one of the production tools of the fertilized eggs, the required number is extremely small, only 3 percent of the total number of the cocks is needed, and the rest cocks are also eliminated. According to investigation of most of the existing laying hen farms in China, many laying hen farms with hatching capacity can sell laying hens after young cocks are hatched, and most laying hens are killed as production raw materials. Therefore, reasonably improving the brooding quantity of the laying hens and enhancing the production characteristics of the laying hens is an effective means for improving the breeding benefit. At present, people mostly improve the egg laying and breeding characteristics of laying hens through late-period strict feeding and other management, generally have complex operation, obviously improve the breeding cost, have a certain effect and low cost performance, and can effectively solve the problem by improving the characteristics of chicks.
Disclosure of Invention
The invention aims to provide an embryo egg hatching method for improving the production characteristics of hens aiming at the existing problems.
The invention is realized by the following technical scheme:
an embryo egg hatching method for improving the production characteristics of hens comprises the following steps:
(1) and (4) incubation for treatment on day 4-5:
when the fertilized hatching eggs are incubated for 4-5 days, performing exogenous nutrient A injection treatment on the fertilized hatching eggs, specifically, wiping and disinfecting the fertilized hatching eggs near an eggshell injection point by using a degreasing cotton ball soaked by 75% alcohol, then penetrating a syringe needle into the fertilized hatching eggs through the eggshell, controlling the penetration depth to be 40-50% of the total length of the minor axis of the fertilized hatching eggs, injecting 0.7-1 ml of exogenous nutrient A, then taking out the syringe needle, and then sealing the eggshell injection point by using paraffin; the exogenous nutrient A comprises the following substances in percentage by weight: 0.05-0.08% of glutamine, 0.03-0.06% of taurine and the balance of physiological saline;
(2) selecting female chick-embryo eggs:
a. when the fertilized eggs are incubated for 8-9 days, carrying out gender identification on the embryonated eggs, and selecting female embryonated eggs from the embryonated eggs for later use;
b. at the moment, the degreased cotton ball soaked by 75% alcohol is wiped and disinfected near an eggshell injection point, and then the long axis of the fertilized hatching egg is fixed in the horizontal direction and is kept still for 14-17 min;
c. penetrating a syringe needle into the egg shell through the egg shell to extract 2-3 ml of allantoic fluid for later use, and finally sealing an injection point of the egg shell by using paraffin;
d. c, centrifuging allantoic fluid collected in the operation c, determining the estrogen concentration of the obtained supernatant by adopting a time-resolved fluoroimmunoassay method, and finally selecting female chicken eggs for later use;
(3) and (3) incubation for 12-13 days:
when the fertilized hatching eggs are incubated for 12-13 days, performing exogenous nutrient B injection treatment on the fertilized hatching eggs, specifically, wiping and disinfecting the fertilized hatching eggs near an eggshell injection point by using a degreasing cotton ball soaked by 75% alcohol, then penetrating a syringe needle into the fertilized hatching eggs through the eggshell, controlling the penetration depth to be 50-55% of the total length of the minor axis of the fertilized hatching eggs, injecting 1.0-1.3 ml of exogenous nutrient B, then taking out the syringe needle, and then sealing the eggshell injection point by using paraffin; the exogenous nutrient B comprises the following substances in percentage by weight: 0.06-0.09% of glutamine, 0.04-0.06% of taurine, 0.02-0.04% of gamma-aminobutyric acid and the balance of normal saline;
(4) and (3) finishing incubation:
and (4) carrying out conventional incubation treatment on the fertilized hatching eggs treated in the step (3), and finishing incubation when the fertilized hatching eggs are incubated for 17-21 days.
Furthermore, the injection points in the step (1), the step (2) and the step (3) are the same, have the same position and are positioned on the outer circumference of the short axis of the fertilization hatching egg.
Further, the diameter of the syringe needle in the step (1), the step (2) and the step (3) is 0.45 mm.
Further, the centrifugal treatment in the operation d in the step (2) is to place the allantoic fluid into a centrifuge with the rotating speed of 2500-2800 rpm for centrifugal treatment for 13-15 min.
Further, in the step (2), the estrogen in the operation d is estradiol E2.
In the hatching process of fertilized eggs, the fertilized eggs correspondingly develop into embryonated eggs of corresponding time through different hatching time, the inner tissue structures of the embryonated eggs of different embryo ages are different just like the development of human fetuses, the splitting and differentiating processes of the fertilized eggs in different periods are different, the natural hatching time of the eggs is about 19 days generally, and chicks with different fertile body qualities are treated aiming at different embryo ages.
According to the invention, when fertilized hatching eggs are incubated for 4-5 days, exogenous nutrient A is injected into the fertilized hatching eggs, wherein glutamine and taurine components are added into the exogenous nutrient A, primordia of epithelial cell mass appear in the upper capsule of the hatching egg cavity during the period, then the primordia develop into a mucosal cavity, the period is the first peak period of the upper capsule development of the cavity, the glutamine and taurine components additionally added at the time can stimulate the differentiation of epithelial cells, the epithelial cells can be largely differentiated to generate lymphocytes, and the lymphocytes can also be differentiated to generate immunoglobulin, so that the premise is provided for the synthesis of the immunoglobulin, and the foundation is laid for the enhancement of the subsequent organism constitution; when the female fertilized eggs are incubated for 8-9 days, sex discrimination and screening are carried out on the fertilized eggs, female fertilized eggs are effectively bred, the blindness of subsequent operation can be avoided, the cost of overall treatment is saved, and support is provided for selective breeding of a farm; and when the eggs are incubated for 12-13 days, the mucosal epithelium of the bursa of the cavity has bud-shaped protrusions of epithelial buds, the epithelial buds are continuously differentiated and grow up and gradually enter into the inherent membrane under the mucosal epithelium, meanwhile, lymphocytes appear in buds and tissues around the buds, and finally, the follicles are formed. While the bleb is formed, the mucosa epithelium is also differentiated into continuous bleb epithelium and interfollicular epithelium, which is the second peak of the bursa development on the cavity, a large number of lymph epithelium blebs are arranged in the mucosa layer of the bursa on the cavity and the differentiation of the mucosa epithelium related to the lymph epithelium, the top of the lymph epithelium blebs face to the mucosa cavity and are closely arranged into layers in the mucosa; the follicle consists of cortex and medulla, with medulla in the center and cortex surrounding the medulla. Medulla comes from mucosa epithelium, multiple protruding reticular epithelial cells form a reticular scaffold, lymphocytes, macrophages, a small amount of plasma cells and granulocytes are filled in the meshes, and then exogenous nutrient B is injected into the meshes, wherein glutamine, taurine and gamma-aminobutyric acid added in the exogenous nutrient B can stimulate the proliferation of the lymphocytes and mononuclear macrophages in the meshes, promote the differentiation of cells, improve the content of immunoglobulin, enhance the physique of chicks, and simultaneously enhance the development of organs such as embryonic intestines, ovaries, heart and lung, and the like, thereby enhancing the original production and breeding performance of the chicks.
Compared with the prior art, the invention has the following advantages:
in the process of hatching the fertilized hatching eggs, the invention reasonably carries out special treatment in different hatching periods, effectively improves the synthetic amount of immunoglobulin in the embryo eggs, improves the development speed and quality of various organs, fully utilizes the original nutrient substances in the fertilized hatching eggs, finally breeds have the advantages of good body quality and strong production characteristics, and have commercial value.
Detailed Description
Example 1
An embryo egg hatching method for improving the production characteristics of hens comprises the following steps:
(1) and (4) incubation for treatment on day 4-5:
when the fertilized hatching eggs are incubated for 4-5 days, performing exogenous nutrient A injection treatment on the fertilized hatching eggs, specifically, wiping and disinfecting the fertilized hatching eggs near an eggshell injection point by using a degreasing cotton ball soaked by 75% alcohol, then penetrating a syringe needle into the fertilized hatching eggs through the eggshell, controlling the penetration depth to be 40% of the total length of the minor axis of the fertilized hatching eggs, injecting 0.7 ml of exogenous nutrient A, then taking out the syringe needle, and then sealing the eggshell injection point by using paraffin; the exogenous nutrient A comprises the following substances in percentage by weight: 0.05% of glutamine, 0.03% of taurine and the balance of normal saline;
(2) selecting female chick-embryo eggs:
a. when the fertilized eggs are incubated for 8-9 days, carrying out gender identification on the embryonated eggs, and selecting female embryonated eggs from the embryonated eggs for later use;
b. at the moment, the degreased cotton ball soaked by 75% alcohol is wiped and disinfected near an eggshell injection point, and then the long axis of the fertilized hatching egg is fixed in the horizontal direction and is kept still for 14-15 min;
c. penetrating a syringe needle into the egg shell through the egg shell to extract 2 ml of allantoic fluid for later use, and finally sealing an injection point of the egg shell by using paraffin;
d. c, centrifuging allantoic fluid collected in the operation c, determining the estrogen concentration of the obtained supernatant by adopting a time-resolved fluoroimmunoassay method, and finally selecting female chicken eggs for later use;
(3) and (3) incubation for 12-13 days:
when the fertilized hatching eggs are incubated for 12-13 days, performing exogenous nutrient B injection treatment on the fertilized hatching eggs, specifically, wiping and disinfecting the fertilized hatching eggs near an eggshell injection point by using a degreasing cotton ball soaked by 75% alcohol, then penetrating a syringe needle into the fertilized hatching eggs through the eggshells, controlling the penetration depth to be 50% of the total length of the minor axis of the fertilized hatching eggs, injecting 1.0 ml of exogenous nutrient B, then taking out the syringe needle, and then sealing the eggshell injection point by using paraffin; the exogenous nutrient B comprises the following substances in percentage by weight: 0.06% of glutamine, 0.04% of taurine, 0.02% of gamma-aminobutyric acid and the balance of physiological saline;
(4) and (3) finishing incubation:
and (4) carrying out conventional incubation treatment on the fertilized hatching eggs treated in the step (3), and finishing incubation when the fertilized hatching eggs are incubated for 17-21 days.
Furthermore, the injection points in the step (1), the step (2) and the step (3) are the same, have the same position and are positioned on the outer circumference of the short axis of the fertilization hatching egg.
Further, the diameter of the syringe needle in the step (1), the step (2) and the step (3) is 0.45 mm.
Further, the centrifugal treatment in operation d of step (2) is to place allantoic fluid in a centrifuge rotating at 2500 rpm for centrifugal treatment for 13 min.
Further, in the step (2), the estrogen in the operation d is estradiol E2.
Example 2
An embryo egg hatching method for improving the production characteristics of hens comprises the following steps:
(1) and (4) incubation for treatment on day 4-5:
when the fertilized hatching eggs are incubated for 4-5 days, performing exogenous nutrient A injection treatment on the fertilized hatching eggs, specifically, wiping and disinfecting the fertilized hatching eggs near an eggshell injection point by using a degreasing cotton ball soaked by 75% alcohol, then penetrating a syringe needle into the fertilized hatching eggs through the eggshells, controlling the penetration depth to be 45% of the total length of the minor axis of the fertilized hatching eggs, injecting 0.9 ml of exogenous nutrient A, then taking out the syringe needle, and then sealing the eggshell injection point by using paraffin; the exogenous nutrient A comprises the following substances in percentage by weight: 0.065% of glutamine, 0.045% of taurine and the balance of physiological saline;
(2) selecting female chick-embryo eggs:
a. when the fertilized eggs are incubated for 8-9 days, carrying out gender identification on the embryonated eggs, and selecting female embryonated eggs from the embryonated eggs for later use;
b. at the moment, the degreased cotton ball soaked by 75% alcohol is wiped and disinfected near an eggshell injection point, and then the long axis of the fertilized hatching egg is fixed in the horizontal direction and is kept still for 15-16 min;
c. penetrating a syringe needle into the egg shell through the egg shell to extract 2.5 ml of allantoic fluid for later use, and finally sealing an injection point of the egg shell by using paraffin;
d. c, centrifuging allantoic fluid collected in the operation c, determining the estrogen concentration of the obtained supernatant by adopting a time-resolved fluoroimmunoassay method, and finally selecting female chicken eggs for later use;
(3) and (3) incubation for 12-13 days:
when the fertilized hatching eggs are incubated for 12-13 days, performing exogenous nutrient B injection treatment on the fertilized hatching eggs, specifically, wiping and disinfecting the fertilized hatching eggs near an eggshell injection point by using a degreasing cotton ball soaked by 75% alcohol, then penetrating a syringe needle into the fertilized hatching eggs through the eggshells, controlling the depth of penetration to be 53% of the total length of the minor axis of the fertilized hatching eggs, injecting 1.2 ml of exogenous nutrient B, then taking out the syringe needle, and then sealing the eggshell injection point by using paraffin; the exogenous nutrient B comprises the following substances in percentage by weight: 0.075% of glutamine, 0.05% of taurine, 0.03% of gamma-aminobutyric acid and the balance of physiological saline;
(4) and (3) finishing incubation:
and (4) carrying out conventional incubation treatment on the fertilized hatching eggs treated in the step (3), and finishing incubation when the fertilized hatching eggs are incubated for 17-21 days.
Furthermore, the injection points in the step (1), the step (2) and the step (3) are the same, have the same position and are positioned on the outer circumference of the short axis of the fertilization hatching egg.
Further, the diameter of the syringe needle in the step (1), the step (2) and the step (3) is 0.45 mm.
Further, the centrifugal treatment in operation d of step (2) is to centrifuge the allantoic fluid in a centrifuge at 2700 rpm for 14 min.
Further, in the step (2), the estrogen in the operation d is estradiol E2.
Example 3
An embryo egg hatching method for improving the production characteristics of hens comprises the following steps:
(1) and (4) incubation for treatment on day 4-5:
when the fertilized hatching eggs are incubated for 4-5 days, performing exogenous nutrient A injection treatment on the fertilized hatching eggs, specifically, wiping and disinfecting the fertilized hatching eggs near an eggshell injection point by using a degreasing cotton ball soaked by 75% alcohol, then penetrating a syringe needle into the fertilized hatching eggs through the eggshells, controlling the penetration depth to be 50% of the total length of the minor axis of the fertilized hatching eggs, injecting 1ml of exogenous nutrient A, then taking out the syringe needle, and then sealing the eggshell injection point by using paraffin; the exogenous nutrient A comprises the following substances in percentage by weight: 0.08 percent of glutamine, 0.06 percent of taurine and the balance of normal saline;
(2) selecting female chick-embryo eggs:
a. when the fertilized eggs are incubated for 8-9 days, carrying out gender identification on the embryonated eggs, and selecting female embryonated eggs from the embryonated eggs for later use;
b. at the moment, the degreased cotton ball soaked by 75% alcohol is wiped and disinfected near the injection point of the eggshell, and then the long axis of the fertilized hatching egg is fixed in the horizontal direction and is kept still for 17 min;
c. penetrating a syringe needle into the egg shell through the egg shell to extract 3ml of allantoic fluid for later use, and finally sealing an injection point of the egg shell by using paraffin;
d. c, centrifuging allantoic fluid collected in the operation c, determining the estrogen concentration of the obtained supernatant by adopting a time-resolved fluoroimmunoassay method, and finally selecting female chicken eggs for later use;
(3) and (3) incubation for 12-13 days:
when the fertilized hatching eggs are incubated for 12-13 days, performing exogenous nutrient B injection treatment on the fertilized hatching eggs, specifically, wiping and disinfecting the fertilized hatching eggs near an eggshell injection point by using a degreasing cotton ball soaked by 75% alcohol, then penetrating a syringe needle into the fertilized hatching eggs through the eggshell, controlling the penetration depth to be 55% of the total length of the minor axis of the fertilized hatching eggs, injecting 1.3ml of exogenous nutrient B, then taking out the syringe needle, and then sealing the eggshell injection point by using paraffin; the exogenous nutrient B comprises the following substances in percentage by weight: 0.09% of glutamine, 0.06% of taurine, 0.04% of gamma-aminobutyric acid and the balance of physiological saline;
(4) and (3) finishing incubation:
and (4) carrying out conventional incubation treatment on the fertilized hatching eggs treated in the step (3), and finishing incubation when the fertilized hatching eggs are incubated for 17-21 days.
Furthermore, the injection points in the step (1), the step (2) and the step (3) are the same, have the same position and are positioned on the outer circumference of the short axis of the fertilization hatching egg.
Further, the diameter of the syringe needle in the step (1), the step (2) and the step (3) is 0.45 mm.
Further, the centrifugal treatment in operation d of step (2) is to centrifuge the allantoic fluid in a centrifuge at 2800 rpm for 15 min.
Further, in the step (2), the estrogen in the operation d is estradiol E2.
Comparative example 1
In comparative example 1, compared with example 2, the taurine component of the exogenous nutrient A was omitted and replaced with glutamine of the same amount in the treatment of day 4 to day 5 after incubation in step (1), except that the other steps of the method were the same.
Comparative example 2
In comparative example 2, compared with example 2, the γ -aminobutyric acid component of the exogenous nutrient B was omitted and replaced with glutamine of the same amount in the treatment of day 12 to day 13 after the incubation in step (3), except that the other steps of the method were the same.
Comparative example 3
In this comparative example 3, the specific operation of the original step (3) was carried out between 14 th and 15 th day of incubation, as compared with example 2, except that the other steps of the method were the same.
Control group
Existing hatching treatment methods.
In order to compare the effects of the invention, a hatching and feeding experiment is designed, specifically, fertilized eggs bred by the same batch of 'Isha brown' laying hens of the same day age are selected as hatching culture objects, the fertilized eggs are divided into five groups at random, then the hatching management is carried out by the method of the embodiment 2, the comparative embodiment 1, the comparative embodiment 2, the comparative embodiment 3 and the control group, then 100 female chicks with similar body types, similar body weights, good mental states and no diseases are selected from the chicks bred by each group, and then the 100 female chicks are put into a henhouse together for breeding management, the specific breeding method is the same and suitable, wherein the weight percentage of each substance in the fed feed is as follows: 60.5% of corn, 0.55% of lard, 13.5% of soybean meal, 2.8% of cottonseed meal, 3.2% of vegetable cake, 3% of peanut meal, 5.5% of guniting bran, 9.3% of stone powder, 0.5% of calcium hydrophosphate, 0.04% of lysine, 0.06% of methionine, 0.02% of liquid phytase, 0.03% of liquid xylanase and 1% of premix; after the chicken is bred into adult hens of 30 weeks old, egg laying and other characteristic statistics are carried out on the later bred chickens for one month, and specific comparison data are shown in the following table 1:
TABLE 1
| Laying rate (%) | Material to egg ratio | Weight of each egg (g/piece) | Breakage Rate (%) | |
| Example 2 | 91.6 | 2.17 | 65.13 | 0.66 |
| Comparative example 1 | 88.1 | 2.31 | 64.65 | 0.69 |
| Comparative example 2 | 87.6 | 2.36 | 64.32 | 0.70 |
| Comparative example 3 | 85.3 | 2.43 | 63.90 | 0.73 |
| Control group | 83.2 | 2.50 | 63.52 | 0.79 |
As can be seen from table 1 above, the chicks bred by the special hatching method of the present invention have good production characteristics such as laying rate and laying quality under the condition of identical subsequent growth conditions, which indicates that the improved operation adopted during hatching has a good effect and has a good promotion effect on the development of the laying hen breeding industry.
Claims (5)
1. An embryo egg hatching method for improving the production characteristics of hens is characterized by comprising the following steps:
(1) and (4) incubation for treatment on day 4-5:
when the fertilized hatching eggs are incubated for 4-5 days, performing exogenous nutrient A injection treatment on the fertilized hatching eggs, specifically, wiping and disinfecting the fertilized hatching eggs near an eggshell injection point by using a degreasing cotton ball soaked by 75% alcohol, then penetrating a syringe needle into the fertilized hatching eggs through the eggshell, controlling the penetration depth to be 40-50% of the total length of the minor axis of the fertilized hatching eggs, injecting 0.7-1 ml of exogenous nutrient A, then taking out the syringe needle, and then sealing the eggshell injection point by using paraffin; the exogenous nutrient A comprises the following substances in percentage by weight: 0.05-0.08% of glutamine, 0.03-0.06% of taurine and the balance of physiological saline;
(2) selecting female chick-embryo eggs:
a. when the fertilized eggs are incubated for 8-9 days, carrying out gender identification on the embryonated eggs, and selecting female embryonated eggs from the embryonated eggs for later use;
b. at the moment, the degreased cotton ball soaked by 75% alcohol is wiped and disinfected near an eggshell injection point, and then the long axis of the fertilized hatching egg is fixed in the horizontal direction and is kept still for 14-17 min;
c. penetrating a syringe needle into the egg shell through the egg shell to extract 2-3 ml of allantoic fluid for later use, and finally sealing an injection point of the egg shell by using paraffin;
d. c, centrifuging allantoic fluid collected in the operation c, determining the estrogen concentration of the obtained supernatant by adopting a time-resolved fluoroimmunoassay method, and finally selecting female chicken eggs for later use;
(3) and (3) incubation for 12-13 days:
when the fertilized hatching eggs are incubated for 12-13 days, carrying out injection treatment on the exogenous nutrient B on the female embryonated eggs, specifically, wiping and disinfecting the degreased cotton balls soaked by 75% alcohol near the injection points of the eggshells, then penetrating the needle heads of the injectors into the eggshells through the eggshells, controlling the penetration depth to be 50-55% of the total length of the minor axis of the fertilized hatching eggs, injecting 1.0-1.3 ml of the exogenous nutrient B, then taking out the needle heads of the injectors, and then carrying out sealing treatment on the injection points of the eggshells by using paraffin; the exogenous nutrient B comprises the following substances in percentage by weight: 0.06-0.09% of glutamine, 0.04-0.06% of taurine, 0.02-0.04% of gamma-aminobutyric acid and the balance of normal saline;
(4) and (3) finishing incubation:
and (4) carrying out conventional incubation treatment on the fertilized hatching eggs treated in the step (3), and finishing incubation when the fertilized hatching eggs are incubated for 17-21 days.
2. The method of claim 1, wherein the injection points of step (1), (2) and (3) are the same injection point, located at the same position, and located on the outer circumference of the minor axis of the fertilized egg.
3. The method of claim 1, wherein the diameter of the needle of the injector in the steps (1), (2) and (3) is 0.45 mm.
4. The method of claim 1, wherein the step (2) comprises centrifuging allantoic fluid in a centrifuge at 2500-2800 rpm for 13-15 min.
5. Method for hatching embryonated eggs for improving the production characteristics of hens according to claim 1, wherein in operation d of step (2) said estrogen is in particular estradiol E2.
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