CN106489835A - The hatching technique of embryo egg - Google Patents
The hatching technique of embryo egg Download PDFInfo
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- CN106489835A CN106489835A CN201610937922.4A CN201610937922A CN106489835A CN 106489835 A CN106489835 A CN 106489835A CN 201610937922 A CN201610937922 A CN 201610937922A CN 106489835 A CN106489835 A CN 106489835A
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of hatching technique of embryo egg, fertilization hatching egg is sent in couveuse, is hatched 10～19 days under dark condition, in the incubation period, to being fertilized, hatching egg is carried out60Co gamma Rays total times are 10～30min, and radiation dose rate is 2～10GY/min.The hatching technique of the present invention can greatly improve the content of immunoglobulin in embryo egg, and especially the content of Yolk immune globulin, so as to greatly improve effect of the resisting fatigue and enhance immunity of embryo egg, very commercially valuable.
Description
Technical field
The present invention relates to the hatching technique of egg, and in particular to the hatching technique of embryo egg.
Background technology
Chicken embryo be fertilized eggs through special hatching technique, make the continuous growth promoter of embryo, have vital egg, and
Referred to as " living pearl ", " hair egg " etc..Actually chicken embryo is exactly also non-broken shell after fertilized eggs are hatched to certain natural law, can
Edible egg.《Compendium of Materia Medica》Record：" chicken embryo has the function of controlling headache, migraine, a crazy disease and the crazy miasma of extremity ".Children and old men
Weak person's often to eat it, have strengthening the spleen and stomach to act on, the effect of play building body then.Chicken embryo delicious flavour, effect are magical, morning among the people
There is food custom, and as nutrition, the good merchantable brand that nourishes and cure the disease, wide-spread.
Modern medicine study proves that chicken embryo ingredient can be generally divided into three classes, i.e. nutritional labeling, infection composition
And bioactive substance.Nutritional labeling has protein, aminoacid, fat, vitamin and mineral etc.；Infection composition has ball egg
In vain, interferon, lysozyme and phosphodiesterase；Bioactive substance has immunoglobulin, sialic acid etc..Immunoglobulin, especially
Which is Yolk immunoglublin IgY, with antibody activity, participates in human body Nutrition and Metabolism and physiological regulation function, can exclude people
Body toxin, defence and kill enter antibacterial and the virus of human body, the effect with resisting fatigue and enhance immunity.Grow in Embryo Gallus domesticus
In growth course, the content of IgY gradually can increase, generally, maintain in the 10th～19 day of incubation period one compared with
High level, generally improves 50% than fresh hen egg, and in fresh hen egg, the content of IgY is 8～10mg/ml.Therefore, the 10th of the incubation period
Suddenly hatching, IgY contents highest in the embryo egg for obtaining, the work(with higher resisting fatigue and enhance immunity are interrupted in～19 days
Effect.
Content of the invention
The technical problem to be solved in the present invention is to provide a kind of embryo for further improving Yolk immune globulin (IgY) content
The hatching technique of egg.
The technical scheme that the present invention is provided is a kind of hatching technique of embryo egg, and fertilization hatching egg is sent in couveuse, black
Hatch 10～19 days under dark condition, in the incubation period, to being fertilized, hatching egg is carried out60Co 10～30min of gamma Rays, radiation dose rate
For 2～10GY/min.
During whole hatching, to being fertilized, hatching egg is carried out60Co gamma Rays total times be 10～30min, radiation dose
Rate is 2～10GY/min.Fertilization hatching egg is chicken fertilization hatching egg, in chicken embryo in incubation period, carries out low dosage60Co gamma-rays spokes
Penetrate, lymphocyte and mononuclear phagocyte propagation can be stimulated, promoted cell differentiation, improved the synthesis of immunoglobulin.
During whole hatching, to being fertilized, hatching egg is carried out60Co gamma Rays total times be 10～30min, radiation dose
Rate is 2～10GY/min.Wherein it is possible at the 4th～5 day of the incubation period, to being fertilized, hatching egg was carried out60Co gamma Rays 5～
10min, radiation dose rate are 2～5GY/min, and residual radiation dosage and radiated time can enter in random time in the incubation period
OK.
There is the former base of epithelial cell group in chicken embryo the 4th～5 day in incubation period, bursa of Fabricius, and followed by former base develops viscous
Membrane cavity, is now first peak period of the development of bursa of Fabricius, during this time egg is carried out60Co gamma Rays, can be with
Stimulate the differentiation of epithelial cell, and epithelial cell can break up generation lymphocyte in a large number, and lymphocyte can break up generation
Immunoglobulin, so as to the synthesis for immunoglobulin provides premise.
During whole hatching, to being fertilized, hatching egg is carried out60Co 10～30min of gamma Rays, radiation dose rate be 2～
10GY/min.Wherein it is possible at the 4th～5 day of the incubation period, to being fertilized, hatching egg was carried out60Co 5～10min of gamma Rays, radiation
Close rate is 2～5GY/min；Further, can be so that at the 12nd～13 day of the incubation period, to being fertilized, hatching egg be carried out60Co γ are penetrated
5～20min of beta radiation, radiation dose rate are 5～10GY/min.
There is the bud shape projection of epithelial sprout, epithelium in chicken embryo the 12nd～13 day in incubation period, the mucous epithelium of bursa of Fabricius
Bud constantly breaks up, grows up, in the lamina propria gradually goed deep under mucous epithelium, meanwhile, lymphocyte is in sprout and around sprout
Tissue in occur, eventually form folliculus.While folliculus is formed, mucous epithelium is also divided into follicle associated epithelium and folliculus therewith
Between epithelium.It is now the second peak of bursa of Fabricius's development, in bursa of Fabricius's mucous layer lamina propria, there are a large amount of lympho epithelial follicles
With the differentiation of mucous epithelium related to this, layer is closely lined up at the top of lympho epithelial follicle towards mucomembranous cavity, in lamina propria；
Folliculus is made up of cortex and medullary substance, and central authorities are medullary substance, and cortex is enclosed within outside medullary substance.Medullary substance derives from mucous epithelium, how raised net
Columnar epithelium cellularity network, is filled with lymphocyte, macrophage and a small amount of plasma cell, granulocyte in mesh.Now
Second is carried out to which60Co gamma Rays can stimulate the propagation of mesh medium-sized lymphocyte and mononuclear phagocyte, promote thin
Born of the same parents break up, so as to produce substantial amounts of immunoglobulin.
During hatching, incubation temperature is controlled for 37.5～38.5 DEG C, humidity is 50～60%.
Fertilization hatching of breeding eggs is advisable for 14～19 days, because during the 12-13 days incubation periods, substantial amounts of macrophage and lymph are thin
Born of the same parents generate, so as to produce substantial amounts of immunoglobulin.
Compared with prior art, hatching technique of the invention can greatly improve the content of immunoglobulin in embryo egg, especially
Which is the content of Yolk immune globulin, so as to greatly improve effect of the resisting fatigue and enhance immunity of embryo egg, extremely has
Commercially valuable.
Specific embodiment
The present invention is further elaborated for specific examples below, but not as a limitation of the invention.
Embodiment 1
The fertilization hatching egg of commercial generation Buddhist nun gram coral powder laying hen is sent in couveuse, is hatched 10 days under dark condition, control
Incubation temperature processed is 37.5 DEG C, and humidity is 50%；At the 1st day of the incubation period, to being fertilized, hatching egg was carried out60Co gamma Rays
10min, radiation dose rate are 2GY/min.
Embodiment 2
Commercial generation Buddhist nun gram coral powder laying hen chicken fertilization hatching egg is sent in couveuse, is hatched 15 days under dark condition, control
Incubation temperature processed is 38.5 DEG C, and humidity is 60%；At the 1st day of the incubation period, to being fertilized, hatching egg was carried out60Co gamma Rays
30min, radiation dose rate are 10GY/min.
Embodiment 3
The fertilization hatching egg of commercial generation Buddhist nun gram coral powder laying hen is sent in couveuse, is hatched 19 days under dark condition, control
Incubation temperature processed is 38 DEG C, and humidity is 55%；At the 19th day of the incubation period, to being fertilized, hatching egg was carried out60Co gamma Rays
20min, radiation dose rate are 5GY/min.
Embodiment 4
The fertilization hatching egg of commercial generation Buddhist nun gram coral powder laying hen is sent in couveuse, is hatched 19 days under dark condition, control
Incubation temperature processed is 38 DEG C, and humidity is 55%；At the 4th day of the incubation period, to being fertilized, hatching egg was carried out60Co gamma Rays 5min,
Radiation dose rate is 2GY/min.At the 19th day of the incubation period, to being fertilized, hatching egg was carried out60Co gamma Rays 5min, radiation dose
Rate is 5GY/min.
Embodiment 5
The fertilization hatching egg of commercial generation Buddhist nun gram coral powder laying hen is sent in couveuse, is hatched 19 days under dark condition, control
Incubation temperature processed is 38 DEG C, and humidity is 55%；At the 4th day of the incubation period, to being fertilized, hatching egg was carried out60Co gamma Rays 10min,
Radiation dose rate is 5GY/min.At the 19th day of the incubation period, to being fertilized, hatching egg was carried out60Co gamma Rays 15min, radiation agent
Dose rate is 2GY/min.
Embodiment 6
The fertilization hatching egg of commercial generation Buddhist nun gram coral powder laying hen is sent in couveuse, is hatched 19 days under dark condition, control
Incubation temperature processed is 38 DEG C, and humidity is 55%；At the 5th day of the incubation period, to being fertilized, hatching egg was carried out60Co gamma Rays 10min,
Radiation dose rate is 5GY/min.At the 13rd day of the incubation period, to being fertilized, hatching egg was carried out60Co gamma Rays 20min, radiation agent
Dose rate is 10GY/min.
Reference examples
The fertilization hatching egg of commercial generation Buddhist nun gram coral powder laying hen is sent in couveuse, hatching hatching 19 under dark condition
My god, incubation temperature is controlled for 38 DEG C, humidity is 55%.
The embryo egg of Example 1～6 and reference examples is each 10 pieces, shells, and detects the content of IgY in every piece of embryo egg one by one, tool
Body result see the table below：
Note：Compared with matched group, * p ＜ 0.05；* p ＜ 0.01.
For detecting the effect of the embryo egg enhance immunity of the application, immunity test is carried out to mice, Details as Follows：
1st, test method
The Embryo Gallus domesticus of embodiment 1～6 and reference examples are precooked, is cooked, is shelled, rubbed, dried, pulverized and sieved plus raw
Reason saline is configured to the embryo egg suspension that concentration is 1.2g/kg respectively.
Immunity test：By 2 monthly age SPF levels KM female white mice 56,7 groups are randomly divided into, respectively 1～6 He of test group
Matched group, 8 per group.Every mice of test group 1～6 feeds the embryo egg that the embryo egg of 0.4ml embodiments 1～6 makes respectively and mixes
Suspension, the embryo egg of every mouse stomach 0.4ml reference examples of matched group make embryo egg suspension.Mice free choice feeding, drinking-water, raise
During supporting, change water daily, the next day change bedding and padding.Daily gavage once, continuous gavage 30d.
2nd, preparation of reagents
Chicken red blood cell CRBC) suspension：Fresh anticoagulant Sanguis Gallus domesticus, put 2500r/min centrifugation 5min, abandon upper plasma and leukocyte
Layer, with 8 times of normal saline dilution erythrocyte, is gently mixed washing, and supernatant is abandoned in suction, and cyclic washing is colourless to supernatant, to remove
The albuminous cell of plasma protein is removed, normal saline, as packed red cells are abandoned in suction to last time as far as possible, according to desired concn, are used
Normal saline dilution chicken red blood cell.
Sheep red blood cell (SRBC) (SRBC) suspension：Sheep venous blood sampling, Sanguis caprae seu ovis is put in the sterilizing conical flask of bead towards one
Individual direction shake, to take off fiber, is put into 4 DEG C of Refrigerator stores standby.
Complement：Collection guinea pig blood, isolates serum (at least pooled serums of 5 Cavia porcelluss), 1ml hematocrit SRBC is added to
In 5ml guinea pig serum, 30min placed by 4 DEG C of refrigerators, often vibrates, and supernatant, subpackage, -70 DEG C of preservations are gone in centrifugation.Used time is slow with SA
Liquid is rushed by 1：10 dilutions.
Hank ' s liquid：NaCl 8g、KCl 0.4g、Na2HPO4·12H2O 0.152g、KH2PO40.06g, phenol red
(0.005g first uses NaHCO3Solution dissolves) 1000ml is settled to distilled water, use NaHCO3Solution adjusts pH value 7.2～7.4,
High temperature sterilize.
3rd, the measure of index and spleen index
After off-test, mice fasting 12h weighs, and takes mouse spleen and weighs, is calculated as follows index and spleen index：Index and spleen index
=spleen weight/body weight；
4th, the measure of Phagocytosis By The Peritoneal Macrophages In Mice
The activation of mouse macrophage：Before test, 4d gives every 2% hematocrit sheep red blood cell (SRBC) 0.2ml of mouse peritoneal injection.
The incubation of macrophage：Mice is put to death with cervical dislocation, lumbar injection adds Hank ' the s liquid of calf serum
4ml/ only, gently rubs abdominal part 20 times, fully to wash out peritoneal macrophage, abdominal cavity is cut off an osculum then, draw abdomen
Chamber washing liquid 0.5ml is mixed with 0.5ml 1%CRBC suspensions, takes 0.5ml mixed liquors, and less than in the agar circle of microscope slide, placement is incubated
Change incubation 15min in device.
Dyeing：Non- attached cell is washed out after terminating rapidly by incubation with normal saline, in methanol solution fixes 1min,
Giemsa liquid dyes 15min, clean with distilled water flushing, dries, and is counted with 40 power microscopes, and be calculated as follows phagocytic rate and
Phagocytic index.
Number of macrophages/200 macrophage × 100% of phagocytic rate=phagocytosis chicken red blood cell
Chicken red blood cell sum/200 macrophages of phagocytic index=phagocytosis
5th, serum hemolysin (IgM) is determined
Immune animal：The cell suspension that hematocrit SRBC normal saline is made into 2%, every mice pay off injection 0.2ml
Carry out immunity.After immune 7d, excision eyeball takes blood and is placed in centrifuge tube, places about 1h, solidification blood stasis tube wall is peeled off, fills serum
Analyze, 2000r/min is centrifuged 10min, collect serum.10 μ l of serum are taken, with 100 times of normal saline dilution.Dilute serum 1ml
With 0.5ml 5%CRBC 10% complements of 0.5ml mix, at 37 DEG C be incubated 30min, terminating reaction on ice-water bath.Centrifuging and taking
Supernatant, colorimetric at the 540nm wavelength, the blank using not increase serum is used as control.
6th, the impact to mice swimming time
After last gavage 30min, by the mice of root of the tail portion load 5% (body weight) sheet lead put swimming trunk (50cm × 50cm ×
40cm) went swimming, 25 DEG C ± 1.0 DEG C of water temperature, record mice start to death time, i.e. mice burden swimming time from swimming
(min).
7th, result of the test
1) impact (x ± s) of the chicken embryo to mouse spleen index
| Group | Index and spleen index (mg/g) |
| Matched group | 3.6134±0.6465 |
| Test 1 group | 5.5261±0.6338* |
| Test 2 groups | 5.4285±0.2334* |
| Test 3 groups | 5.5282±0.4965* |
| Test 4 groups | 6.0921±0.7335** |
| Test 5 groups | 6.0295±0.9591* |
| Test 6 groups | 6.6134±0.3579* |
Note：Compared with matched group, * p ＜ 0.05；* p ＜ 0.01.
2) impact to Phagocytosis By The Peritoneal Macrophages In Mice
| Group | Phagocytic rate | Phagocytic index |
| Matched group | 43.50±3.70 | 0.64±0.07 |
| Test 1 group | 59.75±2.84* | 0.81±0.17* |
| Test 2 groups | 60.75±4.5** | 0.82±0.02* |
| Test 3 groups | 58.00±3.74* | 0.80±0.03* |
| Test 4 groups | 65.50±4.43* | 0.85±0.06** |
| Test 5 groups | 65.16±1.73* | 0.84±0.08* |
| Test 6 groups | 70.25±5.31** | 0.89±0.05* |
Note：Compared with matched group, * p ＜ 0.05；* p ＜ 0.01.
3) impact to mice serum Hemolysin formation
Chicken red blood cell CRBC) suspension：Fresh anticoagulant Sanguis Gallus domesticus, put 2500r/min centrifugation 5min, abandon upper plasma and leukocyte
Layer, with 8 times of normal saline dilution erythrocyte, is gently mixed washing, and supernatant is abandoned in suction, and cyclic washing is colourless to supernatant, to remove
The albuminous cell of plasma protein is removed, normal saline, as packed red cells are abandoned in suction to last time as far as possible, according to desired concn, are used
Normal saline dilution chicken red blood cell.
To CRBC immune mouses so as to which lymphocyte produces the anti-CRBC antibody of specificity, and discharges to peripheral blood, containing antibody
Serum when being incubated together with CRBC, produce haemolysis in the case where complement is participated in and discharge hemoglobin, solution takes on a red color, and determines molten
The absorption value of liquid, calculates half hemolysis value HC50, reflect IgM antibody level.
| Group | Half hemolysis value HC50 |
| Matched group | 428.91±36.43 |
| Test 1 group | 585.35±61.40* |
| Test 2 groups | 576.71±27.89* |
| Test 3 groups | 568.16±26.94* |
| Test 4 groups | 594.98±79.24** |
| Test 5 groups | 599.60±19.17* |
| Test 6 groups | 609.42±94.18* |
Note：Compared with matched group, * p ＜ 0.05；* p ＜ 0.01.
4) impact to mice swimming time
| Group | Time (min) |
| Matched group | 7.67±0.35 |
| Test 1 group | 16.65±1.42 |
| Test 2 groups | 16.72±2.01 |
| Test 3 groups | 16.49±1.31 |
| Test 4 groups | 18.35±2.43 |
| Test 5 groups | 18.26±3.16 |
| Test 6 groups | 19.47±1.43 |
Note：Compared with matched group, * p ＜ 0.05；* p ＜ 0.01.
For detecting the effect of the embryo egg resisting fatigue of the application, anti-fatigue test is carried out to mice, Details as Follows：
1st, test method
The Embryo Gallus domesticus of embodiment 1～6 and reference examples are precooked, is cooked, is shelled, rubbed, dried, pulverized and sieved plus raw
Reason saline is configured to the embryo egg suspension that concentration is 1.2g/kg respectively.
SPF levels Kunming kind male white mouse 210, body weight 20g ± 2g, after white mice raises one week in advance, is randomly divided into 7 groups,
Respectively test group 1～6 and matched group, 30 per group.Every mice of test group 1～6 feeds 0.4ml embodiments 1～6 respectively
The embryo egg suspension made of embryo egg, the embryo egg of every mouse stomach 0.4ml reference examples of matched group makes embryo egg suspension.Little
Mus free choice feeding, drinking-water, in feeding process, change water daily, the next day change bedding and padding.Daily gavage once, continuous gavage 30d.
2nd, Loaned swimming test
After last gavage 30min, by the mice of root of the tail portion load 5% (body weight) sheet lead put swimming trunk (50cm × 50cm ×
40cm) went swimming, 25 DEG C ± 1.0 DEG C of water temperature, record mice start not float to the 8s that swims like a tailor's goose from swimming, i.e. mice swimming time
(min).Start the time to be exhausted to power by only record motion, as mice swimming time, after mice power exhausts, pulled rapidly out water
Pond, in couveuse is dried up to be put in cage with Blowing drum and is normally raised.
3rd, the measure of blood urea nitrogen BUN lactate dehydrogenase L DH
After last gavage 30min, not swimming with a load attached to the body 90min in the water of 30 DEG C ± 1.0 DEG C of water temperature, eye after rest 60min
Ball is taken a blood sample, and isolates serum, and automatic clinical chemistry analyzer determines serum urea nitrogen content.After last gavage 30min, eyeball is adopted
Blood, isolates serum, and automatic clinical chemistry analyzer determines lactic acid dehydrogenase activity.
4th, the measure of hepatic glycogen HG
1) sample preparation
After mice last gavage 30min, in 30 DEG C ± 1.0 DEG C of water went swimming 90min of water temperature, put to death immediately, take liver,
Blotted with filtering residue after trying saline rinse, accurately weigh liver and get over 100mg, add 4mlTCA, often pipe homogenate 1min will homogenate
Liquid pours centrifuge tube into, is centrifuged 15min with 3000r/min, supernatant is moved in another test tube.TCA is added in precipitation, even
Slurry 1min, then 15min is centrifuged, supernatant is removed, and is merged with the supernatant of first time centrifugation, fully mixed.
1ml supernatant is taken, is put in 10ml centrifuge tubes, often pipe adds 95% ethanol 4ml, fully mixes between two kinds of liquid
Interface is not stayed.With clean stopper beyond the Great Wall, vertically stand overnight under room temperature.After precipitation is complete, it is centrifuged in 3000r/min through test tube
15min.Carefully outwell supernatant and make test tube stand upside down and place 10min.
Glycogen is dissolved with 2ml distilled water, when adding water, the glycogen of tube wall is washed down.
Reagent blank：2ml distilled water is inhaled to clean centrifuge tube.
Standard pipe：Inhale 0.5ml glucose standards and 1.5ml distilled water is put in same pipe.
2) sample glycogen content is determined
72%H2SO4：280ml distilled water is added in beaker, concentrated sulphuric acid 720ml is added.
Anthrone reagent：H2SO4In be put into 500mg anthrones, the highly purified thiourea of 10g suitably rocks beaker mixing.After cooling
It is put into refrigerator standby.
10ml anthrone reagents are added sample tube, is fully mixed.From test tube injection anthrone reagent, test tube is placed on
Have a shower under cold water faucet.After all test tubes all reaches cold water temperature, boiling water bath 15min is dipped in, then psychrolusia is moved on to,
It is cooled to room temperature.Liquid in pipe is moved into color comparison tube, with mensuration absorbance after the zeroing of reagent blank pipe under 620nm wavelength.Root
Mass conversion according to the liver for being weighed is into glycogen content.
3) glycogen content is calculated
Glycogen content (mg/100g)=A in hepatic tissue1/A2×0.5×8/0.1×100×0.9；
A in formula1, A2：Difference representative sample QC absorbance and standard pipe absorbance；
0.5：Glucose content in 0.5ml glucose standards；
0.9：The coefficient that glucose is converted into glycogen；
8：Extracting liquid volume, ml；
0.1：Hepatic tissue grams, g.
6th, the measure of blood lactase acid (LD)
After last gavage 30min, 2% (body weight) of bearing a heavy burden stops after the water went swimming 30min of 30 DEG C ± 1.0 DEG C of temperature,
After quiet 15min, eyeball blood sampling, isolates serum, and time-and-motion study absorbance is described in strict accordance with test kit, calculates blood lactase acid and contains
Amount.
6th, result of the test
1) impact of the Embryo Gallus domesticus to mice swimming time
| Group | Swimming time (min) |
| Matched group | 12.52±1.06 |
| Test 1 group | 25.30±3.07* |
| Test 2 groups | 23.08±2.43* |
| Test 3 groups | 26.52±3.50* |
| Test 4 groups | 30.42±2.50** |
| Test 5 groups | 31.95±6.60* |
| Test 6 groups | 35.15±2.82* |
Note：Compared with matched group, * p ＜ 0.05；* p ＜ 0.01.
2) to mice blood urea nitrogen (BUN), the impact of lactic acid dehydrogenase (LDH)
Body blood urea nitrogen content increases with work and the increase of sports load, and body is got over to load performance
Difference, blood urea nitrogen increase are more obvious.
| Group | BUN(mmol/L) | LDH(U/L) |
| Matched group | 9.37±1.08 | 1877.67±134.03 |
| Test 1 group | 6.98±0.58* | 2302.33±147.00 |
| Test 2 groups | 6.89±0.95* | 2377.50±89.80* |
| Test 3 groups | 6.78±0.47* | 2351.33±250.86* |
| Test 4 groups | 6.45±0.68* | 2495.00±104.65* |
| Test 5 groups | 6.52±0.78** | 2405.00±89.10* |
| Test 6 groups | 6.11±0.78* | 2485.00±81.17* |
Note：Compared with matched group, * p ＜ 0.05；* p ＜ 0.01.
3) impact to mice hepatic glycogen (HG) content
Note：Compared with matched group, * p ＜ 0.05；* p ＜ 0.01.
4) impact to mice blood lactase acid (LD) content
The change of Serum lactic acid content illustrates glucolytic degree internal under anaerobic, is to evaluate resisting fatigue to commonly use
Biochemical indicator.
| Group | LD(mmol/L) |
| Matched group | 10.18±1.48 |
| Test 1 group | 7.98±0.77* |
| Test 2 groups | 7.56±1.00* |
| Test 3 groups | 7.73±0.66* |
| Test 4 groups | 6.59±0.84* |
| Test 5 groups | 6.20±0.45* |
| Test 6 groups | 6.01±0.22** |
Note：Compared with matched group, * p ＜ 0.05；* p ＜ 0.01.
Claims (5)
1. the hatching technique of embryo egg, it is characterised in that：Fertilization hatching egg is sent in couveuse, hatches 10～19 under dark condition
My god, in the incubation period, to being fertilized, hatching egg is carried out60Co gamma Rays total times are 10～30min, and radiation dose rate is 2～10GY/
min.
2. the hatching technique of embryo egg according to claim 1, it is characterised in that：At the 4th～5 day of the incubation period, to fertilization
Hatching egg is carried out60Co 5～10min of gamma Rays, radiation dose rate are 2～5GY/min.
3. the hatching technique of embryo egg according to claim 2, it is characterised in that：At the 12nd～13 day of the incubation period, to receiving
Smart hatching egg is carried out60Co 5～20min of gamma Rays, radiation dose rate are 5～10GY/min.
4. the hatching technique of embryo egg according to claim 1, it is characterised in that：Incubation temperature is controlled for 37.5～38.5
DEG C, humidity is 50～60%.
5. the hatching technique of embryo egg according to claim 1, it is characterised in that：Fertilization hatching of breeding eggs 14～19 days.
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