CN106962273A - A kind of embryo egg hatching method for lifting hen industry characteristics - Google Patents
A kind of embryo egg hatching method for lifting hen industry characteristics Download PDFInfo
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- CN106962273A CN106962273A CN201710190262.2A CN201710190262A CN106962273A CN 106962273 A CN106962273 A CN 106962273A CN 201710190262 A CN201710190262 A CN 201710190262A CN 106962273 A CN106962273 A CN 106962273A
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- 230000012447 hatching Effects 0.000 title claims abstract description 106
- 210000001161 mammalian embryo Anatomy 0.000 title claims abstract description 43
- 238000000034 method Methods 0.000 title claims abstract description 28
- 235000013601 eggs Nutrition 0.000 claims abstract description 110
- 230000004720 fertilization Effects 0.000 claims abstract description 33
- 238000012545 processing Methods 0.000 claims abstract description 27
- 238000009395 breeding Methods 0.000 claims abstract description 24
- 230000001488 breeding effect Effects 0.000 claims abstract description 24
- 238000002347 injection Methods 0.000 claims description 49
- 239000007924 injection Substances 0.000 claims description 49
- 102000002322 Egg Proteins Human genes 0.000 claims description 45
- 108010000912 Egg Proteins Proteins 0.000 claims description 45
- 210000003278 egg shell Anatomy 0.000 claims description 45
- 239000003795 chemical substances by application Substances 0.000 claims description 36
- 235000016709 nutrition Nutrition 0.000 claims description 35
- 230000035764 nutrition Effects 0.000 claims description 34
- XOAAWQZATWQOTB-UHFFFAOYSA-N taurine Chemical compound NCCS(O)(=O)=O XOAAWQZATWQOTB-UHFFFAOYSA-N 0.000 claims description 26
- 238000011282 treatment Methods 0.000 claims description 25
- 229920000297 Rayon Polymers 0.000 claims description 15
- 238000004140 cleaning Methods 0.000 claims description 15
- 239000012530 fluid Substances 0.000 claims description 15
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 claims description 15
- 239000012188 paraffin wax Substances 0.000 claims description 15
- 239000002964 rayon Substances 0.000 claims description 15
- 238000007789 sealing Methods 0.000 claims description 15
- 238000004659 sterilization and disinfection Methods 0.000 claims description 15
- BTCSSZJGUNDROE-UHFFFAOYSA-N gamma-aminobutyric acid Chemical compound NCCCC(O)=O BTCSSZJGUNDROE-UHFFFAOYSA-N 0.000 claims description 14
- 239000000126 substance Substances 0.000 claims description 14
- 229960003080 taurine Drugs 0.000 claims description 13
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 10
- 229940011871 estrogen Drugs 0.000 claims description 10
- 239000000262 estrogen Substances 0.000 claims description 10
- 238000001764 infiltration Methods 0.000 claims description 10
- 230000008595 infiltration Effects 0.000 claims description 10
- 239000002504 physiological saline solution Substances 0.000 claims description 8
- 230000008569 process Effects 0.000 claims description 8
- OGNSCSPNOLGXSM-UHFFFAOYSA-N (+/-)-DABA Natural products NCCC(N)C(O)=O OGNSCSPNOLGXSM-UHFFFAOYSA-N 0.000 claims description 7
- 229960003692 gamma aminobutyric acid Drugs 0.000 claims description 7
- VOXZDWNPVJITMN-ZBRFXRBCSA-N 17β-estradiol Chemical compound OC1=CC=C2[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CCC2=C1 VOXZDWNPVJITMN-ZBRFXRBCSA-N 0.000 claims description 5
- 229960005309 estradiol Drugs 0.000 claims description 5
- 229930182833 estradiol Natural products 0.000 claims description 5
- 239000000284 extract Substances 0.000 claims description 5
- 238000003018 immunoassay Methods 0.000 claims description 5
- 239000006228 supernatant Substances 0.000 claims description 5
- 230000035479 physiological effects, processes and functions Effects 0.000 claims description 2
- 239000012266 salt solution Substances 0.000 claims description 2
- 238000005119 centrifugation Methods 0.000 claims 1
- 238000011161 development Methods 0.000 abstract description 6
- 230000018109 developmental process Effects 0.000 abstract description 6
- 108060003951 Immunoglobulin Proteins 0.000 abstract description 5
- 102000018358 immunoglobulin Human genes 0.000 abstract description 5
- 230000008901 benefit Effects 0.000 abstract description 4
- 210000000056 organ Anatomy 0.000 abstract description 3
- 230000000052 comparative effect Effects 0.000 description 12
- 241000287828 Gallus gallus Species 0.000 description 7
- 210000000981 epithelium Anatomy 0.000 description 7
- 238000004519 manufacturing process Methods 0.000 description 7
- 235000013330 chicken meat Nutrition 0.000 description 6
- 239000000203 mixture Substances 0.000 description 6
- 210000004698 lymphocyte Anatomy 0.000 description 5
- 210000002919 epithelial cell Anatomy 0.000 description 4
- 210000001669 bursa of fabricius Anatomy 0.000 description 3
- 230000004069 differentiation Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000002708 enhancing effect Effects 0.000 description 3
- 230000035611 feeding Effects 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000009360 aquaculture Methods 0.000 description 2
- 244000144974 aquaculture Species 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 230000023922 bursa of Fabricius development Effects 0.000 description 2
- 210000002540 macrophage Anatomy 0.000 description 2
- 235000012054 meals Nutrition 0.000 description 2
- 108010011619 6-Phytase Proteins 0.000 description 1
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- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 description 1
- 201000004569 Blindness Diseases 0.000 description 1
- 240000002791 Brassica napus Species 0.000 description 1
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- 241000196324 Embryophyta Species 0.000 description 1
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 description 1
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- FUFJGUQYACFECW-UHFFFAOYSA-L calcium hydrogenphosphate Chemical compound [Ca+2].OP([O-])([O-])=O FUFJGUQYACFECW-UHFFFAOYSA-L 0.000 description 1
- 230000002612 cardiopulmonary effect Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000024245 cell differentiation Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
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- 235000012343 cottonseed oil Nutrition 0.000 description 1
- 235000019700 dicalcium phosphate Nutrition 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
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- 229930182817 methionine Natural products 0.000 description 1
- 210000001672 ovary Anatomy 0.000 description 1
- 235000020232 peanut Nutrition 0.000 description 1
- 229940085127 phytase Drugs 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K45/00—Other aviculture appliances, e.g. devices for determining whether a bird is about to lay
- A01K45/007—Injecting or otherwise treating hatching eggs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Birds (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of embryo egg hatching method for lifting hen industry characteristics, belong to technical field of agricultural breeding, comprise the following steps：（1）Hatch processing in the 4th ~ 5 day,（2）Female embryo in egg selection,（3）Hatch processing in the 12nd ~ 13 day,（4）Hatching is completed.The present invention is during hatching of breeding eggs of being fertilized, reasonably special processing has been carried out in different hatching periods, effectively improve the synthetic quantity of immunoglobulin in embryo egg, improve the rate of development and quality of a variety of organs, it sufficiently make use of nutriment original in fertilization hatching egg, the chick finally brought out has constitution good, the strong advantage of industry characteristics, very commercially valuable.
Description
Technical field
The invention belongs to technical field of agricultural breeding, and in particular to a kind of embryo egg hatching side of lifting hen industry characteristics
Method.
Background technology
The sex of chicken and its productivity are closely bound up.Commodity egg production in, hen as unique tool of production, and
Cock, which does not possess production capacity, to be eliminated.In the production of fertile egg, cock is only used as one of tool of production of fertile egg, institute
Need quantity few, only the 3% of chicken total quantity, remaining cock will also be eliminated.Most breeding layer chickens existing to China are adjusted
Discovery is looked into, many breeding layer chickens with hatchability are sold after cockerel hatching, mostly as raw materials for production quilt
Slaughter.Therefore the quantity of brooding of laying hen hen is reasonably improved, the industry characteristics of enhancing laying hen hen are lifting culture benefits
Effective means.Current many feedings by the later stage strictly of people etc. manage to lift the Breeding trait of laying eggs of laying hen hen, generally
Complex operation, and it has been obviously improved aquaculture cost, although can play a role effect, but cost performance is slightly lower, and to chick
Self character is improved, and can effectively solve this problem.
The content of the invention
The purpose of the present invention is that there is provided a kind of embryo egg hatching side for lifting hen industry characteristics the problem of being directed to existing
Method.
The present invention is achieved by the following technical solutions：
A kind of embryo egg hatching method for lifting hen industry characteristics, comprises the following steps：
（1）Hatch processing in the 4th ~ 5 day：
When hatching of breeding eggs of being fertilized was to 4 ~ 5 days, exogenous nutrition agent A injection treatments are carried out to fertilization hatching egg, are specifically first to use 75% wine
Essence infiltration after rayon balls near eggshell injection point cleaning disinfection, then syringe needle is goed deep into it through eggshell
Portion, it is the 40 ~ 50% of fertilization hatching egg short axle overall length to control deep depth, and exogenous nutrition agent A is injected to take out after 0.7 ~ 1 ml and noted
Emitter syringe needle, then carries out sealing treatment with paraffin to eggshell injection point；Each substance weight percentage in described exogenous nutrition agent A
Than for：Glutamine 0.05 ~ 0.08%, taurine 0.03 ~ 0.06%, surplus is physiological saline；
（2）Female embryo in egg is chosen：
A. when hatching of breeding eggs of being fertilized was to 8 ~ 9 days, sex discrimination is carried out to embryo in egg, and it is standby therefrom to choose female embryo in egg；
B. now first with 75% alcohol infiltrate after rayon balls near eggshell injection point cleaning disinfection, then will be fertilized hatching egg
Major axis fix in the horizontal direction and 14 ~ 17min of stewing process；
C. syringe needle is goed deep into its internal ml of allantoic fluid 2 ~ 3 that extracts through eggshell standby, finally eggshell injected with paraffin
Point carries out sealing treatment；
D. centrifugal treating is carried out to the allantoic fluid obtained by operation c collections, time-resolved fluorescence then is used to the supernatant of gained
Immunoassay determines estrogen concentrations therein, finally selects female embryo in egg standby；
（3）Hatch processing in the 12nd ~ 13 day：
When hatching of breeding eggs of being fertilized was to 12 ~ 13 days, exogenous nutrition agent B injection treatments are carried out to fertilization hatching egg, are specifically first with 75%
Alcohol infiltration after rayon balls near eggshell injection point cleaning disinfection, then syringe needle is goed deep into it through eggshell
Portion, it is the 50 ~ 55% of fertilization hatching egg short axle overall length to control deep depth, and exogenous nutrition agent B is injected and taken out after 1.0 ~ 1.3 ml
Syringe needle, then carries out sealing treatment with paraffin to eggshell injection point；Each substance weight hundred in described exogenous nutrition agent B
Point ratio is：Glutamine 0.06 ~ 0.09%, taurine 0.04 ~ 0.06%, γ-aminobutyric acid 0.02 ~ 0.04%, surplus is physiology
Salt solution；
（4）Hatching is completed：
To step（3）Fertilization hatching egg after processing carries out conventional hatching processing, and when its hatching was to 17 ~ 21 days, hatching is completed.
Further, step（1）, step（2）, step（3）Described in injection point be same injection point, position phase
Together, on the excircle of fertilization hatching egg short axle.
Further, step（1）, step（2）, step（3）Described in syringe needle a diameter of 0.45mm.
Further, step（2）Operation d described in centrifugal treating be by allantoic fluid be put into rotating speed for 2500 ~ 2800 turns/
13 ~ 15min of centrifugal treating in the centrifuge of minute.
Further, step（2）It is specifically estradiol E2 to operate the estrogen described in d.
Fertilized eggs develop into the embryo egg of corresponding time by different brooding time correspondences in hatching process, different
The embryo egg internal organizational structure in embryo age is different, just as human fetus' development, the division that different times embryonated egg is carried out point
Change process is different, and the natural brooding time of usual egg is 19 days or so, and it is fertile poor into constitution to carry out processing for not homeomorphism age
Different chick, the present invention is exactly improved according to this principle to embryo egg hatching process, excellent to bring out original industry characteristics
Laying hen hen.
The present invention is when being fertilized hatching of breeding eggs the 4th ~ 5 day, the exogenous nutrition agent A to its internal injection, wherein in external source battalion
Support in agent A and with the addition of glutamine and taurine composition, the former base of epithelial cell group occurs in hatching egg embryo egg bursa of Fabricius during this period,
Followed by former base develops mucomembranous cavity, and this stage is first peak period of bursa of Fabricius development, the glutamine now additionally added
The differentiation of epithelial cell can be stimulated with taurine composition, and epithelial cell can substantial amounts of differentiation generation lymphocyte, lymphocyte
Generation immunoglobulin can be broken up again, be the enhancing of follow-up body constitution so as to provide premise for the synthesis of immunoglobulin
Lay a good foundation；Hatch the 8th ~ 9 day when, now to fertilization hatching egg carry out sex distinguish screen, effectively select female by
Smart hatching egg, can avoid the blindness of subsequent operation, save the cost of disposed of in its entirety, selectively cultivate and provide for plant again
Support；When hatching the 12nd ~ 13 day, there is the bud shape projection of epithelial sprout in the mucous epithelium of bursa of Fabricius, and epithelial sprout constantly breaks up, length
Greatly, gradually go deep into the tunica propria under mucous epithelium, meanwhile, occur in tissue of the lymphocyte in sprout and around sprout,
Eventually form folliculus.While folliculus formation, mucous epithelium is also divided into follicle associated epithelium and interfollicular epithelium therewith, this stage
It is the second peak of bursa of Fabricius development, there are a large amount of lympho epithelial follicles and related to this in bursa of Fabricius mucous layer tunica propria
Closely layer is lined up in tunica propria in the differentiation of mucous epithelium, the top of lympho epithelial follicle towards mucomembranous cavity；Folliculus by cortex and
Medullary substance is constituted, and center is medullary substance, and cortex is enclosed on outside medullary substance.Medullary substance derives from mucous epithelium, many raised reticular epithelial cells structures
Reticulate and lymphocyte, macrophage and a small amount of thick liquid cell, granulocyte are flooded with support, mesh, now to its internal injection
Exogenous nutrition agent B, wherein the glutamine added in external source nutritional agents B, taurine and γ-aminobutyric acid composition can be stimulated
The propagation of mesh medium size lymphocyte and mononuclear macrophage, promotes the differentiation of cell, lifts the content of immunoglobulin, and enhancing is young
The constitution of chicken, while can strengthen the development of the organs such as embryo's enteron aisle, ovary, cardiopulmonary again, improves the original production of chick and breeds
Performance.
The present invention has advantages below compared with prior art：
The present invention has reasonably carried out special processing in different hatching periods, effectively carried during hatching of breeding eggs of being fertilized
The synthetic quantity of immunoglobulin in embryo egg has been risen, the rate of development and quality of a variety of organs has been improved, sufficiently make use of fertilization
Original nutriment in hatching egg, the chick finally brought out has constitution good, the strong advantage of industry characteristics, very with business valency
Value.
Embodiment
Embodiment 1
A kind of embryo egg hatching method for lifting hen industry characteristics, comprises the following steps：
（1）Hatch processing in the 4th ~ 5 day：
When hatching of breeding eggs of being fertilized was to 4 ~ 5 days, exogenous nutrition agent A injection treatments are carried out to fertilization hatching egg, are specifically first to use 75% wine
Essence infiltration after rayon balls near eggshell injection point cleaning disinfection, then syringe needle is goed deep into it through eggshell
Portion, it is the 40% of fertilization hatching egg short axle overall length to control deep depth, and syringe is taken out after exogenous nutrition agent A is injected into 0.7 ml
Syringe needle, then carries out sealing treatment with paraffin to eggshell injection point；Each substance weight percentage in described exogenous nutrition agent A
For：Glutamine 0.05%, taurine 0.03%, surplus is physiological saline；
（2）Female embryo in egg is chosen：
A. when hatching of breeding eggs of being fertilized was to 8 ~ 9 days, sex discrimination is carried out to embryo in egg, and it is standby therefrom to choose female embryo in egg；
B. now first with 75% alcohol infiltrate after rayon balls near eggshell injection point cleaning disinfection, then will be fertilized hatching egg
Major axis fix in the horizontal direction and 14 ~ 15min of stewing process；
C. syringe needle is goed deep into its internal ml of allantoic fluid 2 that extracts through eggshell standby, finally with paraffin to eggshell injection point
Carry out sealing treatment；
D. centrifugal treating is carried out to the allantoic fluid obtained by operation c collections, time-resolved fluorescence then is used to the supernatant of gained
Immunoassay determines estrogen concentrations therein, finally selects female embryo in egg standby；
（3）Hatch processing in the 12nd ~ 13 day：
When hatching of breeding eggs of being fertilized was to 12 ~ 13 days, exogenous nutrition agent B injection treatments are carried out to fertilization hatching egg, are specifically first with 75%
Alcohol infiltration after rayon balls near eggshell injection point cleaning disinfection, then syringe needle is goed deep into it through eggshell
Portion, it is the 50% of fertilization hatching egg short axle overall length to control deep depth, and syringe is taken out after exogenous nutrition agent B is injected into 1.0 ml
Syringe needle, then carries out sealing treatment with paraffin to eggshell injection point；Each substance weight percentage in described exogenous nutrition agent B
For：Glutamine 0.06%, taurine 0.04%, γ-aminobutyric acid 0.02%, surplus is physiological saline；
（4）Hatching is completed：
To step（3）Fertilization hatching egg after processing carries out conventional hatching processing, and when its hatching was to 17 ~ 21 days, hatching is completed.
Further, step（1）, step（2）, step（3）Described in injection point be same injection point, position phase
Together, on the excircle of fertilization hatching egg short axle.
Further, step（1）, step（2）, step（3）Described in syringe needle a diameter of 0.45mm.
Further, step（2）Centrifugal treating described in operation d is that allantoic fluid is put into rotating speed for 2500 revs/min
Centrifuge in centrifugal treating 13min.
Further, step（2）It is specifically estradiol E2 to operate the estrogen described in d.
Embodiment 2
A kind of embryo egg hatching method for lifting hen industry characteristics, comprises the following steps：
（1）Hatch processing in the 4th ~ 5 day：
When hatching of breeding eggs of being fertilized was to 4 ~ 5 days, exogenous nutrition agent A injection treatments are carried out to fertilization hatching egg, are specifically first to use 75% wine
Essence infiltration after rayon balls near eggshell injection point cleaning disinfection, then syringe needle is goed deep into it through eggshell
Portion, it is the 45% of fertilization hatching egg short axle overall length to control deep depth, and syringe is taken out after exogenous nutrition agent A is injected into 0.9 ml
Syringe needle, then carries out sealing treatment with paraffin to eggshell injection point；Each substance weight percentage in described exogenous nutrition agent A
For：Glutamine 0.065%, taurine 0.045%, surplus is physiological saline；
（2）Female embryo in egg is chosen：
A. when hatching of breeding eggs of being fertilized was to 8 ~ 9 days, sex discrimination is carried out to embryo in egg, and it is standby therefrom to choose female embryo in egg；
B. now first with 75% alcohol infiltrate after rayon balls near eggshell injection point cleaning disinfection, then will be fertilized hatching egg
Major axis fix in the horizontal direction and 15 ~ 16min of stewing process；
C. syringe needle is goed deep into its internal ml of allantoic fluid 2.5 that extracts through eggshell standby, finally eggshell injected with paraffin
Point carries out sealing treatment；
D. centrifugal treating is carried out to the allantoic fluid obtained by operation c collections, time-resolved fluorescence then is used to the supernatant of gained
Immunoassay determines estrogen concentrations therein, finally selects female embryo in egg standby；
（3）Hatch processing in the 12nd ~ 13 day：
When hatching of breeding eggs of being fertilized was to 12 ~ 13 days, exogenous nutrition agent B injection treatments are carried out to fertilization hatching egg, are specifically first with 75%
Alcohol infiltration after rayon balls near eggshell injection point cleaning disinfection, then syringe needle is goed deep into it through eggshell
Portion, it is the 53% of fertilization hatching egg short axle overall length to control deep depth, and syringe is taken out after exogenous nutrition agent B is injected into 1.2 ml
Syringe needle, then carries out sealing treatment with paraffin to eggshell injection point；Each substance weight percentage in described exogenous nutrition agent B
For：Glutamine 0.075%, taurine 0.05%, γ-aminobutyric acid 0.03%, surplus is physiological saline；
（4）Hatching is completed：
To step（3）Fertilization hatching egg after processing carries out conventional hatching processing, and when its hatching was to 17 ~ 21 days, hatching is completed.
Further, step（1）, step（2）, step（3）Described in injection point be same injection point, position phase
Together, on the excircle of fertilization hatching egg short axle.
Further, step（1）, step（2）, step（3）Described in syringe needle a diameter of 0.45mm.
Further, step（2）Centrifugal treating described in operation d is that allantoic fluid is put into rotating speed for 2700 revs/min
Centrifuge in centrifugal treating 14min.
Further, step（2）It is specifically estradiol E2 to operate the estrogen described in d.
Embodiment 3
A kind of embryo egg hatching method for lifting hen industry characteristics, comprises the following steps：
（1）Hatch processing in the 4th ~ 5 day：
When hatching of breeding eggs of being fertilized was to 4 ~ 5 days, exogenous nutrition agent A injection treatments are carried out to fertilization hatching egg, are specifically first to use 75% wine
Essence infiltration after rayon balls near eggshell injection point cleaning disinfection, then syringe needle is goed deep into it through eggshell
Portion, it is the 50% of fertilization hatching egg short axle overall length to control deep depth, and syringe needle is taken out after exogenous nutrition agent A is injected into 1 ml
Head, then carries out sealing treatment with paraffin to eggshell injection point；Each substance weight percentage is in described exogenous nutrition agent A：
Glutamine 0.08%, taurine 0.06%, surplus is physiological saline；
（2）Female embryo in egg is chosen：
A. when hatching of breeding eggs of being fertilized was to 8 ~ 9 days, sex discrimination is carried out to embryo in egg, and it is standby therefrom to choose female embryo in egg；
B. now first with 75% alcohol infiltrate after rayon balls near eggshell injection point cleaning disinfection, then will be fertilized hatching egg
Major axis fix in the horizontal direction and stewing process 17min；
C. syringe needle is goed deep into its internal ml of allantoic fluid 3 that extracts through eggshell standby, finally with paraffin to eggshell injection point
Carry out sealing treatment；
D. centrifugal treating is carried out to the allantoic fluid obtained by operation c collections, time-resolved fluorescence then is used to the supernatant of gained
Immunoassay determines estrogen concentrations therein, finally selects female embryo in egg standby；
（3）Hatch processing in the 12nd ~ 13 day：
When hatching of breeding eggs of being fertilized was to 12 ~ 13 days, exogenous nutrition agent B injection treatments are carried out to fertilization hatching egg, are specifically first with 75%
Alcohol infiltration after rayon balls near eggshell injection point cleaning disinfection, then syringe needle is goed deep into it through eggshell
Portion, it is the 55% of fertilization hatching egg short axle overall length to control deep depth, and syringe is taken out after exogenous nutrition agent B is injected into 1.3 ml
Syringe needle, then carries out sealing treatment with paraffin to eggshell injection point；Each substance weight percentage in described exogenous nutrition agent B
For：Glutamine 0.09%, taurine 0.06%, γ-aminobutyric acid 0.04%, surplus is physiological saline；
（4）Hatching is completed：
To step（3）Fertilization hatching egg after processing carries out conventional hatching processing, and when its hatching was to 17 ~ 21 days, hatching is completed.
Further, step（1）, step（2）, step（3）Described in injection point be same injection point, position phase
Together, on the excircle of fertilization hatching egg short axle.
Further, step（1）, step（2）, step（3）Described in syringe needle a diameter of 0.45mm.
Further, step（2）Centrifugal treating described in operation d is that allantoic fluid is put into rotating speed for 2800 revs/min
Centrifuge in centrifugal treating 15min.
Further, step（2）It is specifically estradiol E2 to operate the estrogen described in d.
Comparative example 1
This comparative example 1 compared with Example 2, in step（1）Hatch the ox that exogenous nutrition agent A is saved in processing in the 4th ~ 5 day
Sulfonic acid composition, is replaced, method and step all same in addition with the glutamine of equivalent.
Comparative example 2
This comparative example 2 compared with Example 2, in step（3）Hatch in processing in the 12nd ~ 13 day and save exogenous nutrition agent B's
γ-aminobutyric acid composition, is replaced, method and step all same in addition with the glutamine of equivalent.
Comparative example 3
This comparative example 3 compared with Example 2, by former step（3）Concrete operations move to the 14th ~ 15 day of hatching between enter
OK, method and step all same in addition.
Control group
Existing hatching processing method.
In order to contrast effect of the present invention, a kind of hatching feeding experiment is devised, the identical age in days of same batch is specifically selected
" Yi Sha is brown " laying hen educate fertilized eggs as hatching Object of Development, described fertilized eggs are divided into five groups at random, so
Afterwards hatching pipe is carried out with the above embodiments 2, comparative example 1, comparative example 2, comparative example 3, the method for control group
Reason, then selects that build is similar, body weight is close, the state of mind good, disease-free female chick is each from the chick that each group is brought out
100, then it is put into hen house jointly and carries out aquaculture management, specific cultural method is identical and suitable, wherein feeding
The percentage by weight composition of each material is in feed：Corn 60.5%, lard 0.55%, dregs of beans 13.5%, Cottonseed Meal 2.8%, rapeseed cake
3.2%th, peanut meal 3%, whitewashing wheat bran 5.5%, stone flour 9.3%, calcium monohydrogen phosphate 0.5%, lysine 0.04%, methionine 0.06%, liquid
Phytase 0.02%, liquid xylanase 0.03%, premix 1%；After the adult hen for being bred as 30 week old, then to after
One month raised carries out the statistics of features such as lay eggs, and specific correction data is as shown in table 1 below：
Table 1
| Laying rate（%） | Feedstuff-egg ratio | Individual egg counterpoise（G/ pieces） | Breakage rate（%） | |
| Embodiment 2 | 91.6 | 2.17 | 65.13 | 0.66 |
| Comparative example 1 | 88.1 | 2.31 | 64.65 | 0.69 |
| Comparative example 2 | 87.6 | 2.36 | 64.32 | 0.70 |
| Comparative example 3 | 85.3 | 2.43 | 63.90 | 0.73 |
| Control group | 83.2 | 2.50 | 63.52 | 0.79 |
The chick through being educated using the special hatching method of the present invention it can be seen from upper table 1, in the complete phase of subsequent growth condition
With in the case of, the industry characteristics such as its laying rate, quality of laying eggs have good lifting, show the improvement operation used in hatching
Good effect is served, there is good facilitation to the development of breeding layer chicken industry.
Claims (5)
1. a kind of embryo egg hatching method for lifting hen industry characteristics, it is characterised in that comprise the following steps：
（1）Hatch processing in the 4th ~ 5 day：
When hatching of breeding eggs of being fertilized was to 4 ~ 5 days, exogenous nutrition agent A injection treatments are carried out to fertilization hatching egg, are specifically first to use 75% wine
Essence infiltration after rayon balls near eggshell injection point cleaning disinfection, then syringe needle is goed deep into it through eggshell
Portion, it is the 40 ~ 50% of fertilization hatching egg short axle overall length to control deep depth, and exogenous nutrition agent A is injected to take out after 0.7 ~ 1 ml and noted
Emitter syringe needle, then carries out sealing treatment with paraffin to eggshell injection point；Each substance weight percentage in described exogenous nutrition agent A
Than for：Glutamine 0.05 ~ 0.08%, taurine 0.03 ~ 0.06%, surplus is physiological saline；
（2）Female embryo in egg is chosen：
A. when hatching of breeding eggs of being fertilized was to 8 ~ 9 days, sex discrimination is carried out to embryo in egg, and it is standby therefrom to choose female embryo in egg；
B. now first with 75% alcohol infiltrate after rayon balls near eggshell injection point cleaning disinfection, then will be fertilized hatching egg
Major axis fix in the horizontal direction and 14 ~ 17min of stewing process；
C. syringe needle is goed deep into its internal ml of allantoic fluid 2 ~ 3 that extracts through eggshell standby, finally eggshell injected with paraffin
Point carries out sealing treatment；
D. centrifugal treating is carried out to the allantoic fluid obtained by operation c collections, time-resolved fluorescence then is used to the supernatant of gained
Immunoassay determines estrogen concentrations therein, finally selects female embryo in egg standby；
（3）Hatch processing in the 12nd ~ 13 day：
When hatching of breeding eggs of being fertilized was to 12 ~ 13 days, exogenous nutrition agent B injection treatments are carried out to fertilization hatching egg, are specifically first with 75%
Alcohol infiltration after rayon balls near eggshell injection point cleaning disinfection, then syringe needle is goed deep into it through eggshell
Portion, it is the 50 ~ 55% of fertilization hatching egg short axle overall length to control deep depth, and exogenous nutrition agent B is injected and taken out after 1.0 ~ 1.3 ml
Syringe needle, then carries out sealing treatment with paraffin to eggshell injection point；Each substance weight hundred in described exogenous nutrition agent B
Point ratio is：Glutamine 0.06 ~ 0.09%, taurine 0.04 ~ 0.06%, γ-aminobutyric acid 0.02 ~ 0.04%, surplus is physiology
Salt solution；
（4）Hatching is completed：
To step（3）Fertilization hatching egg after processing carries out conventional hatching processing, and when its hatching was to 17 ~ 21 days, hatching is completed.
2. a kind of embryo egg hatching method for lifting hen industry characteristics according to claim 1, it is characterised in that step
（1）, step（2）, step（3）Described in injection point be same injection point, position is identical, positioned at the outer of fertilization hatching egg short axle
On circumference.
3. a kind of embryo egg hatching method for lifting hen industry characteristics according to claim 1, it is characterised in that step
（1）, step（2）, step（3）Described in syringe needle a diameter of 0.45mm.
4. a kind of embryo egg hatching method for lifting hen industry characteristics according to claim 1, it is characterised in that step
（2）Centrifugal treating described in operation d is that allantoic fluid is put into the centrifuge that rotating speed is 2500 ~ 2800 revs/min at centrifugation
Manage 13 ~ 15min.
5. a kind of embryo egg hatching method for lifting hen industry characteristics according to claim 1, it is characterised in that step
（2）It is specifically estradiol E2 to operate the estrogen described in d.
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| CN112715426A (en) * | 2021-01-18 | 2021-04-30 | 温州科技职业学院 | Chick embryo incubation design capable of being observed and transferred |
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