CN104644552A - Intra-embryonic provision tonic capable of promoting growth of broiler chicken and application of intra-embryonic provision tonic - Google Patents
Intra-embryonic provision tonic capable of promoting growth of broiler chicken and application of intra-embryonic provision tonic Download PDFInfo
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Abstract
The invention relates to a method for promoting growth of a broiler chicken through intra-embryonic provision of pyrro-quinoline quinone (PQQ) and application of pyrro-quinoline quinone. 1ml of disodium pyrro-quinoline quinone (PQQ.Na2) solution (with the concentration of 1.0-4.0mM) is injected into a chick embryo air sac in a seventh embryo development stage during incubation by adopting a continuous injector used for injecting vaccine, the effects of increasing hatch weight of the broiler chicken, increasing market weight of the broiler chicken by 110g and increasing brisket weight by 50g are realized, and feed efficiency in the whole stage is improved by more than 5%; besides, stimulation of the air sac injection on embryo development is small, hatchability is not influenced, operation is simple, and working efficiency is high, so that the air sac injection is applicable to being used in large-scale production, and economic benefit is considerable; and a method for scientifically and reasonably applying intra-embryonic pyyro-quinoline quinone (PQQ) provision has a potential application space in intensive poultry production and has great significance in the poultry industry of China.
Description
Technical field
The present invention relates to provisions tonic and application thereof in a kind of embryo promoting growth of meat chicken, belong to poultry early nutrition technical field in livestock breeding industry.
Background technology
Along with social economy is fast-developing, living standards of the people improve constantly, and day by day increase the demand of meat products, and in the past between 30 years, Fowl meat consumption in the world's grows steadily, and this is relatively cheap with Fowl meat price and more easily obtain relevant.Producing 1kg Fowl meat needs feedstuff to be about 2kg, and produces 1kg Carnis Sus domestica or beef and mutton needs feedstuff 3.5 ~ 8kg.The modern poultry speed of growth is constantly accelerated, and the production cycle shortens further, and the ratio shared by the incubation period increases relatively, and if the incubation period of broiler and meat-type duck is close to more than 35% of whole life cycle, and listing age in days from now on also can continue to shorten.Poultry incubation period just accounts for more than 1/3rd of life cycle, if nutrition regulation measure can be taked to promote poultry early development at incubation period, will to after its birth so that lifelong productivity all there is facilitation.In recent years, the nutrition in the birds incubation period is paid attention to day by day.Current, poultry nutrition scholar passes through and finds new method to strengthen the early nutrition of poultry, to promote quick growth and the muscle deposition of broiler, meets the ever-increasing demand to high-quality Fowl meat of consumer.
In embryo, provisions is exactly a kind of early stage provisions scheme of poultry with application prospect.Provisions in embryo, also known as provisions in ovum or extreme early nutrition, is a kind of new method added to by nutritional solution by external source injection in incubation period avian embryonic, reaching facilitating digestion road growth promoter, improves the effect that chickling goes out shell and grow subsequently.In embryo, provisions research starts from nineteen eighty-two, amino acid complex is expelled in embryonic yolk sac at hatching 8d by Al-Murrani, find that injection aminoacid can significantly improve later stage embryo weight, and after going out shell in 56d each stage body weight average pole be significantly higher than matched group, the average weight of 56d is than matched group height 116g.On this basis, Uni and Ferket have extensively studied the amniotic cavity injection method of poultry hatching later stage (broiler is hatching the 17.5th day, and turkey is hatching the 23rd day), and has applied for technical patent.At present except amniotic cavity, the air chamber of embryo egg, the white of an egg, yolk sac are also applied to provisions in ovum, and have the effect promoting fetal development and late growing stage.In embryo, provisions all has facilitation to skeleton, muscle, immune system etc. after broiler hatching.Along with the progress of embryo egg injection technique, poultry nutrition study on regulation is advanceed to period of embryo, and in ovum, provisions has become the feasible method improving poultry Early nutritional state.
Pyrro-quinoline quinone (PQQ) (pyrroloquinoline quinone, has another name called Methoxatin), being called for short PQQ, is nicotinamide nucleotide (NAD
+and NADP
+) and flavin nucleotide (FAD and FMN) outside the third organic prothetic group, belong to a kind of novel biostearin, its molecular weight is 330 dalton, and its structural formula is shown in Fig. 1.PQQ branches away at first from antibacterial, finds that it is almost present in all biological tissues subsequently.Numerous research shows, PQQ is that a kind of plant growing promotes the factor, is also the important nutrient of growth of animal, development and fecundity.Think at present, animal and human's body intestinal microbial population can not synthesize PQQ or synthetic quantity can not meet body requirement far away, needs to obtain from food.Up to now, the research of PQQ physiological function is at home and abroad extensively carried out, and wherein PQQ promotes that animal and plant growth, growth and the effect of breeding are given prominence to, and it can not only shorten the pollen germination time of plant, can also promote that tobacco seed is sprouted, improve lipase active and seed breathing rate.Nutritional studies shows that PQQ is the required trophic factors of growth of animal, development and fecundity.Mice lacks that PQQ there will be that skin fragility, depilation, health are bending, abdominal part is hemorrhage even dead etc. typically lacks symptom.When the research of the present inventor on laying hen finds that in diet, PQQ addition is 0.01mg/ (d only), the laying rate of laying hen and Egg Quality can increase, and the anti-oxidation stress ability of laying hen also obviously strengthens.
PQQ is a kind of water solublity biostearin, generally uses with the form of salt or ester, pyrro-quinoline quinone (PQQ) disodium (PQQ.Na in production
2) be produce at present and the principal mode of PQQ in application, what it played a role is still PQQ itself at all.
PQQ has been listed in a kind of important nutrient, and it is confirmed to the effect of animal body growth and resistance aspect in numerous research.The present inventor's research also finds to add 0.2mg/kg PQQ.Na in Broiler Dietary
2broiler can be made to deliver body weight for sale and increase more than 130g, can improve feed efficiency simultaneously and improve trunk composition, chest muscle weight increases.
Summary of the invention
The object of the invention is to: find according to the present inventor's early-stage Study, pyrro-quinoline quinone (PQQ) has good promotion broiler ontogeny, increase and deliver this feature of body weight for sale, by the method using continuous injector to inject to chick embryo air sac to the 7th age in days hatching embryo, pyrro-quinoline quinone (PQQ) is applied to provisions in embryo, promotion growth of meat chicken can be reached, reduce the effect of broiler breeding cost, significantly improve economic benefit.
Embodiment of the present invention are:
1. the preparation method of provisions pyrroloquinoline quinone (PQQ) in the embryo promoting growth of meat chicken, it is characterized in that by pyrro-quinoline quinone (PQQ) and/or its pharmaceutically acceptable salt or ester in the environment of lucifuge with concentration 65 DEG C of heating for dissolving of 1.0 ~ 4.0mM in sterile physiological injection/or PBS/ or cell culture fluid, then to keep in Dark Place after microporous filter membrane is degerming; 37.5 DEG C are heated to before injection.
2. the application of provisions pyrro-quinoline quinone (PQQ) in a kind of embryo promoting growth of meat chicken as claimed in claim 1, is characterized in that pyrroloquinoline quinone and/or its pharmaceutically acceptable salt or ester solution to be applied in Embryo Gallus domesticus to inject.
3. the application of provisions pyrro-quinoline quinone (PQQ) in a kind of embryo promoting growth of meat chicken as claimed in claim 2, is characterized in that the method using continuous injector to inject in Embryo Gallus domesticus.
4. the application of provisions pyrro-quinoline quinone (PQQ) in a kind of embryo promoting growth of meat chicken as claimed in claim 2, it is characterized in that pyrro-quinoline quinone (PQQ) solution contains the pyrro-quinoline quinone (PQQ) of safe and effective amount and/or its pharmaceutically acceptable salt and/or ester as active component, active component content is 1.0 ~ 4.0mM.
5. the application of provisions pyrro-quinoline quinone (PQQ) in a kind of embryo promoting growth of meat chicken as claimed in claim 2, is characterized in that being applied in the broiler incubation period.
Specific embodiment of the invention method:
1. this nutritional supplement is contained:
(1) pyrro-quinoline quinone (PQQ) of safe and effective amount and/or its pharmaceutically acceptable salt and/or ester are as active component, and active component content is 1.0 ~ 4mM, can be natural, non-natural for PQQ of the present invention.Preferably PQQ can be prepared by synthetic or fermentable.
(2) pharmaceutically acceptable carrier or diluent combine, as carboxymethyl cellulose, normal saline, PBS, glucose solution, cell culture fluid etc.
2. containing provisions injection preparation in the embryo of this nutritional supplement
By pyrro-quinoline quinone (PQQ) or its salt, ester in the environment of lucifuge with concentration 65 DEG C of heating for dissolving of 1.0 ~ 4.0mM in physiological saline solution (0.85%NaCl solution, or pharmaceutically acceptable carrier or diluent combine, as carboxymethyl cellulose, normal saline, PBS, glucose solution, cell culture fluid etc.), keep in Dark Place after degerming again, before injection, be heated to 37.5 DEG C.
3. containing provisions injection store method in the embryo of this nutritional supplement
Use following method for preserving containing provisions injection in the embryo of pyrro-quinoline quinone (PQQ), growth-promoting effect after good hatching can be obtained.Adopt brown glass container to hold or outer housing black protective sleeve, deposit in shady and cool ventilation place, the holding time does not surpass 1 week.
4. promote the test of growth of meat chicken effect containing provisions injection in the embryo of this nutritional supplement.
In employing chick embryo amnoitic sac, provisions beta-hydroxy-β methylbutanoic acid (HMB) is as positive control, any tonic is not given for negative contrast in producing, by the observation to indexs such as fertility performance, Slaughter, immune organ and blood biochemicals, find that in the embryo containing pyrro-quinoline quinone (PQQ), provisions tonic significantly can promote the growth of broiler, and increase chest muscle output, with chick embryo amnoitic sac provisions HMB group zero difference, and some aspect is still better than positive control group, show that this nutritional supplement promotes that the definite effect of growth of meat chicken is reliable after hatching.
In embryo, provisions (In Ovo Feeding) comes from egg inoculation technology.Egg inoculation makes the chickling hatched have a technology of immune protective efficiency to some viral vaccine of living of egg inoculation in hatching later stage.The current comparative maturity of this technology, and comparatively ten-strike is achieved in the application of poultry early immune, the broiler enterprise of the U.S. nowadays more than 80% adopts egg inoculation as the method for Marek’s disease (MD) vaccine immunity.In embryo, provisions refers to a kind of method of injecting nutrient substance before chickling goes out shell and between the incubation period in hatching egg.In embryo, provisions can promote intestinal growth, improves bone health, improves meat yield, improves fertility performance, strengthens immunologic function, promotes that the chickling newly hatched does sth. in advance growth 2 ~ 3 days.Multinomial research shows, amino acid complex, vitamin, mineral etc. all cast in Embryo Gallus domesticus by the mode of provisions in embryo, incubation rate, strong young rate can not only be improved, its growth vigor lasts till that growth stage has increases broiler market weight, reduce the effect of death rate, obviously increase cultivation profit.In Embryo Gallus domesticus, provisions has been the new technique extensively studied both at home and abroad and progressively use at present.
Affect a lot of because have of provisions effect in embryo, as the selection of inject time, injection site, the volume of injection and osmotic pressure and disinfectant measure etc. all can have impact to the growth performance after incubation rate after provisions and hatching.By test, the present inventor finds that air chamber is too small when 0 ~ 4 age in days embryo, should not operate; And Embryo Gallus domesticus starts atrophy at hatching later stage allantochorion, osmosis starts to weaken, therefore is expelled in air chamber and can reduces effect after 15 age in days embryos.Relatively to the effect of 0 age in days embryo and 7 age in days embryo air chambers injection amino acid complex, find 0 age in days embryo injection incubation rate reduce and embryo age on the 7th without impact, therefore embryo on the 7th is the better time of provisions in embryo age.Test shows, inject the growth that aminoacid can have a strong impact on Embryo Gallus domesticus, and operation requirements is high in allantoic cavity and amniotic cavity.The allogenic material injected by air chamber is absorbed by embryo due to osmosis, air chamber injection can reduce to greatest extent because of injecting fluid and operation to embryo produce stress, use that the operation of vaccinate continuous injector is more simple, conveniently, efficiency is high.In broiler embryo, the injection volume of provisions is that 1.6% of egg size is suitable for, the average 65g of egg size, therefore the volume of broiler more options injection is 1mL.
Calculate to use provisions PQQ in embryo between 2000 broiler incubation periods, broiler is delivered for sale great and increases hair chicken income from sales about 2000 yuan, chicken food consumption branched go out about 200 yuan, nutrition fluid infusion, continuous injector, paraffin and cost of labor about 200 yuan during provisions in embryo, income 1600 yuan will be had a net increase of, about every chicken additional income 0.8 yuan.China is poultry farming big country, the annual broiler amount of delivering for sale about 80 ~ 9,000,000,000, if can in the large-scale broiler breeding enterprise application of annual total amount of livestock for sale ten million, economic benefit be very considerable.In scientific and reasonable use embryo, the method for provisions pyrro-quinoline quinone (PQQ) (PQQ) has potential application space in intensive poultry production, is significant to China's domestic fowl farming.
Accompanying drawing explanation
Fig. 1 wherein Fig. 1-A is pyrro-quinoline quinone (PQQ) structure chart; Fig. 1-B is pyrro-quinoline quinone (PQQ) disodium salt structure chart.
Fig. 2 wherein Fig. 2-A and 2-B is provisions application drawing in embryo.
Positive effect of the present invention
The present invention relates to method and the application thereof of provisions pyrro-quinoline quinone (PQQ) (PQQ) in a kind of embryo promoting growth of meat chicken, use vaccinate continuous injector to hatching the 7th embryo instar chicken embryo air chamber injection 1mL pyrro-quinoline quinone (PQQ) disodium (PQQ.Na
2) (concentration is 2mM) solution, reach and broiler hatching body weight is increased and market weight raising 110g, brisket heavily increases the effect of 50g, and full phase feed efficiency improves more than 5%.In addition, the air chamber injection that the present invention proposes stimulates little to fetal development, and do not affect incubation rate, it is easy and simple to handle, and work efficiency is high, and be applicable to scale aborning and use, economic benefit is very considerable.In scientific and reasonable use embryo, the method for provisions pyrro-quinoline quinone (PQQ) (PQQ) has potential application space in intensive poultry production, is significant to China's domestic fowl farming.
Embodiment
Describe the present invention below in conjunction with detailed description of the invention, material agents used in the test method in following example and test examples, if no special instructions, is commercially available conventional method and conventional material and reagent.
Embodiment 1
---the preparation of provisions tonic in embryo of the present invention
Pyrro-quinoline quinone (PQQ) disodium (is presented by shanghai Medicine life science center, chemosynthesis is produced) in the environment of lucifuge with 2mM concentration 65 DEG C of heating for dissolving in physiological saline solution (0.85%NaCl solution), then to keep in Dark Place after microporous filter membrane is degerming.
Embodiment 2
---the injecting method (gas erebro ventricular injection) of provisions pyrro-quinoline quinone (PQQ) in embryo
The operating procedure of the injecting method of provisions pyrro-quinoline quinone (PQQ) is as follows: according to during egg, hatching embryo is moved to operating board at meat hatching the 7th age in days embryo, embryo egg air chamber profile is described fast with pencil, with rayon balls cleaning disinfection near injection point that 75% ethanol infiltrates, hole at air chamber top micro-drilling device, use paraffin sealing immediately after using syringe needle long 8mm continuous injector injection 1ml, whole process completes and hatching egg is put back to incubator and continues hatching in 15min.
Embodiment 3
---the injecting method (amniotic cavity injection) of provisions pyrro-quinoline quinone (PQQ) in embryo
At the 17.5th age in days of meat hatching, hatching embryo is moved to operating board, to break forth with candler the position of embryo, determine amniotic cavity position and use pencil at the standardized ringlet in inserting needle position, with rayon balls cleaning disinfection near injection point that 75% ethanol infiltrates, hole with micro-drilling device hole device in inserting needle position, use the disposable syringe injection 1mL of the long 15mm of syringe needle, use paraffin sealing immediately, whole process completed and hatching egg is put back to incubator and continues hatching in 15 minutes.
Embodiment 4
---containing the dose-response test promoting growth of meat chicken after hatching in pyrro-quinoline quinone (PQQ) embryo to nutrient solution
1. EXPERIMENTAL DESIGN and experimental animal arrangement
(1) incubation period
Buy same age in days kind chicken same day by Parent stock field and produced commercial generation fertile egg 3000 pieces, choose 2100 pieces of hatching egg (egg size 60 ~ 66g, average heavy 63.2g) be assigned randomly to 7 groups, often organize the 300 pieces: 1st group and do not inject any material (matched group); Pyrro-quinoline quinone (PQQ) disodium (the PQQ.Na of variable concentrations (0.5,1,2,4,4.5mM) is injected to air chamber for 2nd to the 6th group in hatching 7 embryo age
2) solution 1mL, the 7th group in hatching 17.5 embryo age to amniotic cavity injection PQQ.Na
2solution (2mM) 1mL.Commodity in use broiler couveuse, incubation condition is: temperature (37.8 ± 0.1) DEG C, relative humidity 60%, and every 1.5h egg-turning is (angle 270 °) once, within the 19th day, stops egg-turning.Hatching house's temperature is (25.0 ± 1.0) DEG C, relative temperature 60% ~ 65%.Hatch the 10th day photograph egg, remove infertile egg and addled egg.Injection before with 70% ~ 75% ethanol infiltration rayon balls cleaning disinfection near injection point, syringe needle length with 1/2 of hatching egg minor axis slightly length be advisable, injected and used paraffin sealing.
(2) stage is raised
After broiler goes out shell, from the broiler of acceptance 7 kinds hatching process, respectively choose 96 healthy public young birds, each process 8 repetition, each repetition 12 chickens.Feeding period totally 42 days, is divided into early stage (0 ~ 21 day) and two stages of later stage (21 ~ 42 days).Feeding and management is undertaken by " AA feeding of broiler administrative manual " (being provided by feeding of broiler field), and bilayer is raised in cages, and natural lighting adds artificially feed, free choice feeding and drinking-water, broiler chicken pelleted ration of feeding.
2. performance test and data statistics
Hatching same day, be that a unit weighs with every 50, calculate average birth weight.The raising stage records the feed intake of broiler 0 ~ 21,22 ~ 42 and 0 ~ 42d, body weight gains in units of repeating, and calculates feed conversion rate.
Data represent with average ± standard deviation (mean ± SD), first adopt the one-way ANOVA of SPSS (15.0) software to carry out the homogeneity inspection of variance, then carry out F inspection and Duncan multiple comparisons.Take P<0.05 as significant difference.
3. result of the test
In table 1 embryo, provisions various dose pyrro-quinoline quinone (PQQ) sodium is on the impact of meat chicken production performance
Note: different lowercase alphabet of going together shows significant difference (P ﹤ 0.05).
In embryo provisions various dose pyrro-quinoline quinone (PQQ) sodium on the impact of meat chicken production performance in table 1.As seen from table, air chamber or amniotic cavity provisions PQQ.Na
2do not affect the incubation rate of broiler, but in embryo, provisions group broiler hatching weight average is significantly higher than matched group, comparatively matched group body weight improves about 1.7 ~ 3g (P ﹤ 0.05).The body weight of broiler during 7 age in days, provisions group comparatively matched group height about 3 ~ 15g in embryo, with 2mM PQQ.Na
2air chamber provisions group body weight is the highest.Broiler 21 age in days body weight, except 4.5mM PQQ.Na
2outside air chamber provisions group, other provisions group body weight are all greater than matched group (P>0.05).Average daily gain in early stage, with 2mM PQQ.Na
2air chamber provisions group is the highest, and this group, a little more than matched group (P<0.10), is secondly 4mM PQQ.Na
2air chamber provisions group and 2mM PQQ.Na
2amniotic cavity provisions group.Early-stage fodder efficiency aspect, between each group relatively, with 2mM PQQ.Na
2air chamber provisions group feed efficiency is optimum.Broiler 42 age in days body weight, except 0.5,4.5mM PQQ.Na
2outside air chamber provisions group, other are respectively organized numerically all higher than matched group, with 2mM PQQ.Na
2air chamber provisions group is the highest, is secondly 2mM PQQ.Na
2amniotic cavity provisions group and 4mMPQQ.Na
2amniotic cavity provisions group.Later stage average daily gain Changing Pattern and 42 age in days body weight similar, 2mM PQQ.Na
2air chamber provisions group and 2mM PQQ.Na
2amniotic cavity provisions group is a little more than matched group (P<0.10), and this feed efficiency of 2 groups is also better.Test the fertility performance of full phase, with 2mM PQQ.Na
2air chamber provisions group and 2mM PQQ.Na
2the group average daily gain of amniotic cavity provisions and feed efficiency the best, and the former feed efficiency is slightly better than matched group (P<0.10).
The present embodiment shows, provisions PQQ.Na in embryo
2there is suitable provisions dosage.In 1mL provisions solution, 7 embryo air chamber in age provisions PQQ.Na
2suitable concentration be 1.0 ~ 4.0mM, 17.5 embryo amniotic cavity in age provisions PQQ.Na
2suitable concentration be 2mM.In this provisions dosage range, containing PQQ.Na
2after promoting hatching to nutrient solution in embryo, growth of meat chicken effect is better.
Embodiment 5
---containing the effect test promoting growth of meat chicken after hatching in pyrro-quinoline quinone (PQQ) embryo to nutrient solution
1. EXPERIMENTAL DESIGN and experimental animal arrangement
(1) incubation period
1200 pieces of AA chicken commercial generation fertile eggs (egg size 65 ~ 70g, on average heavy 66.1g) are randomized to either 4 groups, often organize 300 pieces: group I does not inject any material (matched group); Group II injects pyrro-quinoline quinone (PQQ) disodium (PQQ.Na to air chamber at hatching 7 embryo age
2) solution, group III in hatching 17.5 embryo age to amniotic cavity injection pyrro-quinoline quinone (PQQ) disodium (PQQ.Na
2) solution, organize IV at hatching 17.5 age in days to amniotic cavity injection beta-hydroxy-Beta-methyl butanoic acid (HMB) solution.Commodity in use broiler couveuse, incubation condition is: temperature (37.8 ± 0.1) DEG C, relative humidity 60%, and every 1.5h egg-turning is (angle 270 °) once, within the 19th day, stops egg-turning.Hatching house's temperature is (25.0 ± 1.0) DEG C, relative temperature 60% ~ 65%.Hatch the 10th day photograph egg, remove infertile egg and addled egg.Injection before with 70% ~ 75% ethanol infiltration rayon balls cleaning disinfection near injection point, syringe needle length with 1/2 of hatching egg minor axis slightly length be advisable, injected and used paraffin sealing.
(2) stage is raised
After broiler goes out shell, from the broiler of acceptance 4 kinds hatching process, respectively choose 72 healthy public young birds, each process 6 repetition, each repetition 12 chickens.Feeding period totally 42 days, is divided into early stage (0 ~ 21 day) and two stages of later stage (21 ~ 42 days).Feeding and management is undertaken by " AA feeding of broiler administrative manual ", and bilayer is raised in cages, and natural lighting adds artificially feed, free choice feeding and drinking-water, broiler chicken pelleted ration of feeding.
2. testing index and method
(1) fertility performance
Hatching same day, be that a unit weighs with every 50, calculate average birth weight.The raising stage records the feed intake of broiler 0 ~ 21,22 ~ 42 and 0 ~ 42d, body weight gains in units of repeating, and calculates feed conversion rate.
(2) Slaughter
From each repetition, choose 1 chickling close to average weight at 1,4,7 ages in days respectively, peel off left pectoral after putting to death and weigh, calculate chest muscle rate.From each repetition, choose 1 broiler close to average weight at 21,42 ages in days, cervical region blood-letting is butchered, and depilation drains rear title slaughter traits, measures dressing percentage, complete clean thorax rate, chest muscle rate, lower limb flesh rate and abdominal fat subsequently.
(3) Immune Organs Index
When 21 and 42 ages in days butcher chicken, win immune organ (thymus, spleen and fabricius bursa), weigh and calculate each shoot formation.Shoot formation (g/kg)=organ weight (g)/live body heavy (kg).
(4) blood parameters
When 21 and 42 ages in days butcher chicken, jugular vein blood collection 3mL, anticoagulant heparin pipe is deposited, and the centrifugal 10min of 3000r/min, gets supernatant subpackage, puts-20 DEG C of Refrigerator stores stand-by.Glucose (GLU), creatinine (CRE), uric acid (UA) content adopt automatic clinical chemistry analyzer to measure, and test kit is purchased from Shanghai Kehua Bio-engineering Co., Ltd.
(5) data statistics
Adopt the one-way ANOVA of SPSS (15.0) software first to carry out the homogeneity inspection of variance, then carry out F inspection and Duncan multiple comparisons.(P<0.05 is significant difference, and P ﹤ 0.01 is that difference is extremely remarkable), represents with average ± standard deviation (mean ± SD).
3. result of the test
(1) in embryo provisions pyrro-quinoline quinone (PQQ) sodium on the impact of meat chicken production performance
In embryo provisions pyrro-quinoline quinone (PQQ) sodium on the impact of meat chicken production performance in table 2.Compared with matched group, 7 embryo air chamber in age provisions PQQ.Na
2broiler body weight when 0,21 and 42 age in days improve 2.91g (P ﹤ 0.01) respectively, 12.12g and 110.04g; Provisions PQQ.Na in embryo
2or HMB broiler weight average pole of coming into being is significantly higher than matched group (P ﹤ 0.01).Compared with matched group, air chamber provisions PQQ.Na
2be significantly increased with the average daily gain of amniotic cavity provisions HMB broiler 0 ~ 21d, 21 ~ 42d, between each group, average daily ingestion amount and feed efficiency are all without significant difference (P>0.05).Feed efficiency aspect, though each process is without the significant difference of statistics, in embryo, provisions group feed efficiency is all better than matched group, tests provisions PQQ.Na in full phase embryo
2group feed efficiency comparatively matched group improves 5 ~ 8%, optimum with 7 embryo air chamber in age provisions group feed efficiencies.In embryo, provisions pyrro-quinoline quinone (PQQ) sodium and beta-hydroxy-Beta-methyl butanoic acid can both promote broiler ontogeny, increase market weight, do not observe good and bad therebetween.
In table 2. embryo, provisions pyrro-quinoline quinone (PQQ) sodium is on the impact of meat chicken production performance
Note: different lowercase alphabet of going together shows significant difference (P ﹤ 0.05), lower same.
(2) in embryo provisions pyrro-quinoline quinone (PQQ) sodium on the impact of broiler Slaughter
In embryo provisions pyrro-quinoline quinone (PQQ) sodium on the impact of broiler Slaughter in table 3.As shown in Table 3, embryo egg air chamber provisions PQQ.Na
2group chicken chest rate 1 age in days, 4 ages in days, 7 ages in days, 21 ages in days and 42 age in days time-divisions do not improve 0.68% (P ﹤ 0.05), 0.96% (P ﹤ 0.01), 0.15%, 0.47% and 3.33% (P ﹤ 0.05), in embryo, the heavy comparatively matched group of broiler 42 age in days chest muscle respectively organized by provisions increases more than 50g.During 21 age in days, there are no significant the change of complete clean thorax rate between each processed group, lower limb flesh rate, abdominal fat, but in embryo provisions group complete clean thorax rate, chest muscle rate and lower limb flesh rate numerically all higher than matched group; During 42 age in days, embryo egg air chamber provisions PQQ.Na
2the complete clean thorax rate of group is higher than matched group (P ﹤ 0.10), and chest muscle rate is significantly higher than matched group (P ﹤ 0.05).Between each group lower limb flesh rate and abdominal fat when 42 age in days without significant difference (P>0.05).
In table 3. embryo, provisions pyrro-quinoline quinone (PQQ) sodium is on the impact of broiler Slaughter
(3) in embryo provisions pyrro-quinoline quinone (PQQ) sodium on the impact of broiler immune organ
In embryo provisions pyrro-quinoline quinone (PQQ) sodium on the impact of broiler Development of Immune Organs in table 4.As shown in Table 4, in embryo, provisions pyrro-quinoline quinone (PQQ) does not all make significant difference (P>0.05) to 21 ages in days and 42 Day-old Broiler Chickens thymus indexs, index and spleen index and bursal index.
In table 4. embryo, provisions pyrro-quinoline quinone (PQQ) is on the impact of broiler immune organ
(4) in embryo provisions pyrro-quinoline quinone (PQQ) sodium on the impact of broiler blood parameters
Provisions PQQ.Na in embryo
2table 5 is listed on the impact of chickens blood parameters.As shown in Table 5, in embryo, provisions slightly affects the blood parameters after broiler chicken hatching, does not make significant difference to the blood parameters of Later growth.Air chamber provisions PQQ.Na during 21 age in days
2group broiler Glucose in Blood by Cyclic is higher than other group (P ﹤ 0.01).Blood creatinine aspect, amniotic cavity provisions PQQ.Na
2group broiler 4 age in days, significantly lower than other each group, is significantly higher than other each group, air chamber provisions PQQ.Na during 7 age in days when 7 ages in days and 21 age in days
2group and amniotic cavity provisions HMB group are significantly lower than matched group (P ﹤ 0.05).Blood uric acid aspect, air chamber provisions PQQ.Na
2to go out after shell 1 age in days and 4 ages in days significantly lower than other each group (P ﹤ 0.05)
From the present embodiment result of the test, provisions PQQ.Na in the 7th age in days during incubation and the 17.5th age in days embryo
2to after broiler hatching, all there is obvious growth promoting function, particularly chicken grow early stage, its growth vigor can extend to the later stage, can significantly improve broiler and deliver body weight for sale, improve economic benefit.Result of the test of the present invention shows, 7 age in days embryo air chamber provisions PQQ.Na
2the 17.5 age in days embryo amniotic cavity provisions HMB effects of generally acknowledging growth promoting function and the industry of broiler are suitable, and some index is even slightly better than the latter, has the advantages such as easy to operation, with low cost simultaneously.
In table 5. embryo, provisions pyrro-quinoline quinone (PQQ) sodium is on the impact of broiler blood parameters
Claims (5)
1. the preparation method of provisions pyrroloquinoline quinone (PQQ) in the embryo promoting growth of meat chicken, it is characterized in that pyrro-quinoline quinone (PQQ) and/or its pharmaceutically acceptable salt or ester to be heated in the environment of lucifuge 65 DEG C makes it be dissolved in sterile physiological injection/or PBS/ or cell culture fluid, its final concentration is 1.0 ~ 4.0mM, then keeps in Dark Place after microporous filter membrane is degerming; 37.5 DEG C are heated to before injection.
2. the application of provisions pyrro-quinoline quinone (PQQ) in a kind of embryo promoting growth of meat chicken as claimed in claim 1, is characterized in that pyrroloquinoline quinone and/or its pharmaceutically acceptable salt or ester solution to be applied in Embryo Gallus domesticus to inject.
3. the application of provisions pyrro-quinoline quinone (PQQ) in a kind of embryo promoting growth of meat chicken as claimed in claim 2, is characterized in that the method using continuous injector to inject in Embryo Gallus domesticus.
4. the application of provisions pyrro-quinoline quinone (PQQ) in a kind of embryo promoting growth of meat chicken as claimed in claim 2, it is characterized in that pyrro-quinoline quinone (PQQ) solution contains the pyrro-quinoline quinone (PQQ) of safe and effective amount and/or its pharmaceutically acceptable salt and/or ester as active component, active component content is 1.0 ~ 4.0mM.
5. the application of provisions pyrro-quinoline quinone (PQQ) in a kind of embryo promoting growth of meat chicken as claimed in claim 2, is characterized in that being applied to broiler.
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| CN106107195A (en) * | 2016-06-28 | 2016-11-16 | 中国农业科学院饲料研究所 | A kind of additive package promoting growth of meat chicken and application thereof |
| CN106962273A (en) * | 2017-03-28 | 2017-07-21 | 枞阳县横山生态农业有限公司 | A kind of embryo egg hatching method for lifting hen industry characteristics |
| CN107047459A (en) * | 2017-03-28 | 2017-08-18 | 枞阳县横山生态农业有限公司 | A kind of hatching method for lifting broiler growth characteristic |
| CN113575795A (en) * | 2021-08-18 | 2021-11-02 | 攀枝花学院 | In-ovo nutritional supplement for improving growth performance of newborn poultry and application |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106107195A (en) * | 2016-06-28 | 2016-11-16 | 中国农业科学院饲料研究所 | A kind of additive package promoting growth of meat chicken and application thereof |
| CN106962273A (en) * | 2017-03-28 | 2017-07-21 | 枞阳县横山生态农业有限公司 | A kind of embryo egg hatching method for lifting hen industry characteristics |
| CN107047459A (en) * | 2017-03-28 | 2017-08-18 | 枞阳县横山生态农业有限公司 | A kind of hatching method for lifting broiler growth characteristic |
| CN113575795A (en) * | 2021-08-18 | 2021-11-02 | 攀枝花学院 | In-ovo nutritional supplement for improving growth performance of newborn poultry and application |
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| CN104644552B (en) | 2018-01-09 |
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