CN106360421A - Preparation process of embryo egg health product capable of improving antifatigue efficacy - Google Patents
Preparation process of embryo egg health product capable of improving antifatigue efficacy Download PDFInfo
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- CN106360421A CN106360421A CN201610951478.1A CN201610951478A CN106360421A CN 106360421 A CN106360421 A CN 106360421A CN 201610951478 A CN201610951478 A CN 201610951478A CN 106360421 A CN106360421 A CN 106360421A
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a preparation process of an embryo egg health product capable of improving the antifatigue efficacy. The preparation process comprises the following steps of 1) feeding impregnated hatching eggs into a hatching apparatus, performing incubation for 10-19 days under a dark condition, and performing 60C0 gamma-ray radiation on the impregnated hatching eggs with the radiation dose rate of 12-20GY/min for 5-10minutes during the incubation period; 2) freezing incubated embryo eggs at a temperature of -20 to -30 DEG C for 24-36 hours; and 3) shelling, mashing and drying the froozen embryo eggs, thereby obtaining the embryo egg health products. The preparation method is capable of greatly improving the content of immunoglobulin in the embryo eggs, especially the content of yolk immunoglobulin, and thus greatly improving the antifatigue efficacy of the embryo egg products and improving the immunity efficacy, and has quite favorable commercial value.
Description
Technical field
The present invention relates to a kind of processing technology of egg product is and in particular to improve the embryo egg health product of anti-fatigue effect
Processing technology.
Background technology
Chicken embryo be fertilized eggs through special hatching technique, make the continuous growth promoter of embryo, have vital egg, and
It is referred to as " pearl of living ", " hair egg " etc..Actually chicken embryo is exactly that fertilized eggs are hatched to certain natural law, and also non-broken shell can
Edible egg.Compendium of Material Medica is recorded: " chicken embryo has the function of controlling headache, migraine, the crazy sick and crazy miasma of extremity ".Children and old men
Weak person's often to eat it, have strengthening the spleen and stomach to act on, the effect of then play building body.Chicken embryo delicious flavour, effect is magical, morning among the people
Have a food custom, and as nutrition, the good merchantable brand nourishing and cure the disease, wide-spread.
Modern medicine study proves, chicken embryo ingredient can be generally divided into three classes, i.e. nutritional labeling, infection composition
And bioactive substance.Nutritional labeling has protein, aminoacid, fat, vitamin and mineral etc.；Infection composition has ball egg
In vain, interferon, lysozyme and phosphodiesterase；Bioactive substance has immunoglobulin, sialic acid etc..Immunoglobulin, especially
It is Yolk immunoglublin igy, has antibody activity, participates in human body Nutrition and Metabolism and physiological regulation function, can exclude people
Body toxin, defends and kills the antibacterial entering human body and virus, have the effect of resisting fatigue and enhance immunity.In Embryo Gallus domesticus growth
In growth course, the content of igy can be gradually increased, and generally, maintains one relatively in the 10th～19 day of incubation period
High level, generally improves 50% than fresh hen egg, and in fresh hen egg, the content of igy is 8～10mg/ml.Therefore, the 10th of the incubation period
Suddenly interrupt hatching, igy content highest in the embryo egg obtaining in～19 days, there is the work(of higher resisting fatigue and enhance immunity
Effect.
Content of the invention
The technical problem to be solved in the present invention is to provide one kind to improve carrying of Yolk immune globulin (igy) content further
The processing technology of the embryo egg health product of highly anti-fatigue effect.
The present invention provide technical scheme be a kind of improve anti-fatigue effect embryo egg health product processing technology, including with
Lower step:
1) hatching egg of being fertilized is sent in couveuse, hatches 10～19 days under dark condition, in the incubation period, to fertilization hatching egg
Carry out60Co gamma Rays total time is 5～10min, and radiation dose rate is 12～20gy/min；
2) embryo egg after hatching freezes 24～36h at -20～-30 DEG C；
3) embryo egg after freezing is shelled, smash to pieces, be dried, obtain final product.
During whole hatching, fertilization hatching egg is carried out60Co gamma Rays total time is 5～10min, radiation dose rate
For 12～20gy/min.Fertilization hatching egg is chicken fertilization hatching egg, in chicken embryo in incubation period, carries out low dosage60Co gamma-rays spoke
Penetrate, lymphocyte and mononuclear phagocyte propagation can be stimulated, promote cell differentiation, improve the synthesis of immunoglobulin.
During whole hatching, fertilization hatching egg is carried out60Co gamma Rays total time is 5～10min, radiation dose rate
For 12～20gy/min.Wherein it is possible at the 4th～5 day of the incubation period, carry out to fertilization hatching egg60Co gamma Rays 2～
5min, radiation dose rate is 12～15gy/min, and residual radiation dosage and radiated time can be in random times in the incubation period
Carry out.
Chicken embryo at the 4th～5 day of incubation period, bursa of Fabricius occur epithelial cell group former base, followed by former base develop viscous
Membrane cavity, is now first peak period of the growth of bursa of Fabricius, during this time egg is carried out60Co gamma Rays, permissible
Stimulate the differentiation of epithelial cell, and epithelial cell can break up generation lymphocyte in a large number, and lymphocyte can break up generation
Immunoglobulin, thus provide premise for the synthesis of immunoglobulin.
During whole hatching, fertilization hatching egg is carried out60Co gamma Rays total time is 5～10min, radiation dose rate
For 12～20gy/min.Wherein it is possible at the 4th～5 day of the incubation period, carry out to fertilization hatching egg60Co gamma Rays 2～
5min, radiation dose rate is 12～15gy/min；Further, can also be in the 12nd～13 of incubation period day, to fertilization hatching egg
Carry out60Co gamma Rays 3～5min, radiation dose rate is 15～20gy/min.
At the 12nd～13 day of incubation period, the bud shape projection of epithelial sprout, epithelium to chicken embryo in the mucous epithelium of bursa of Fabricius
Bud constantly breaks up, grows up, and gradually gos deep in the lamina propria under mucous epithelium, and meanwhile, lymphocyte is in sprout and around sprout
Tissue in occur, eventually form folliculus.While folliculus is formed, mucous epithelium is also divided into follicle associated epithelium and folliculus therewith
Between epithelium.It is now the second peak that bursa of Fabricius develops, in bursa of Fabricius's mucous layer lamina propria, have a large amount of lympho epithelial follicles
With the differentiation of mucous epithelium related to this, layer, towards mucomembranous cavity, is closely lined up in lamina propria in the top of lympho epithelial follicle；
Folliculus is made up of cortex and medullary substance, and central authorities are medullary substance, and cortex is enclosed within outside medullary substance.Medullary substance derives from mucous epithelium, how raised net
Columnar epithelium cellularity network, is filled with lymphocyte, macrophage and a small amount of plasma cell, granulocyte in mesh.Now
Second is carried out to it60Co gamma Rays can stimulate the propagation of mesh medium-sized lymphocyte and mononuclear phagocyte, promotes thin
Born of the same parents break up, thus producing substantial amounts of immunoglobulin.
During hatching, incubation temperature is controlled to be 37.5～38.5 DEG C, humidity is 50～60%.
Fertilization hatching of breeding eggs is advisable for 14～19 days, because during the 12-13 days incubation periods, substantial amounts of macrophage and lymph are thin
Born of the same parents generate, thus producing substantial amounts of immunoglobulin.
Compared with prior art, the present invention can greatly improve the content of immunoglobulin in embryo egg, and especially egg yolk is exempted from
The content of epidemic disease globulin, thus greatly improving the resisting fatigue of embryo egg product and effect of enhance immunity, extremely has business
It is worth.
Specific embodiment
The present invention is further elaborated for specific examples below, but not as a limitation of the invention.
Embodiment 1
1) the fertilization hatching egg of commercial generation Buddhist nun gram coral powder laying hen is sent in couveuse, hatches 10 days under dark condition,
Incubation temperature is controlled to be 37.5 DEG C, humidity is 50%；At the 1st day of the incubation period, fertilization hatching egg is carried out60Co gamma Rays
5min, radiation dose rate is 12gy/min；
2) embryo egg after hatching freezes 24h at -20 DEG C；
3) embryo egg after freezing is shelled, smash to pieces, be dried, obtain final product.
Embodiment 2
1) commercial generation Buddhist nun gram coral powder laying hen chicken fertilization hatching egg is sent in couveuse, hatches 15 days under dark condition,
Incubation temperature is controlled to be 38.5 DEG C, humidity is 60%；At the 1st day of the incubation period, fertilization hatching egg is carried out60Co gamma Rays
10min, radiation dose rate is 20gy/min；
2) embryo egg after hatching freezes 36h at -30 DEG C；
3) embryo egg after freezing is shelled, smash to pieces, be dried, obtain final product.
Embodiment 3
1) the fertilization hatching egg of commercial generation Buddhist nun gram coral powder laying hen is sent in couveuse, hatches 19 days under dark condition,
Incubation temperature is controlled to be 38 DEG C, humidity is 55%；At the 19th day of the incubation period, fertilization hatching egg is carried out60Co gamma Rays
8min, radiation dose rate is 15gy/min；
2) embryo egg after hatching freezes 32h at -25 DEG C；
3) embryo egg after freezing is shelled, smash to pieces, be dried, obtain final product.
Embodiment 4
1) the fertilization hatching egg of commercial generation Buddhist nun gram coral powder laying hen is sent in couveuse, hatches 19 days under dark condition,
Incubation temperature is controlled to be 38 DEG C, humidity is 55%；At the 4th day of the incubation period, fertilization hatching egg is carried out60Co gamma Rays
2min, radiation dose rate is 12gy/min.At the 19th day of the incubation period, fertilization hatching egg is carried out60Co gamma Rays 3min, spoke
Penetrating close rate is 15gy/min；
2) embryo egg after hatching freezes 36h at -20 DEG C；
3) embryo egg after freezing is shelled, smash to pieces, be dried, obtain final product.
Embodiment 5
1) the fertilization hatching egg of commercial generation Buddhist nun gram coral powder laying hen is sent in couveuse, hatches 19 days under dark condition,
Incubation temperature is controlled to be 38 DEG C, humidity is 55%；At the 4th day of the incubation period, fertilization hatching egg is carried out60Co gamma Rays
5min, radiation dose rate is 15gy/min.At the 19th day of the incubation period, fertilization hatching egg is carried out60Co gamma Rays 5min, spoke
Penetrating close rate is 20gy/min；
2) embryo egg after hatching freezes 36h at -20 DEG C；
3) embryo egg after freezing is shelled, smash to pieces, be dried, obtain final product.
Embodiment 6
1) the fertilization hatching egg of commercial generation Buddhist nun gram coral powder laying hen is sent in couveuse, hatches 19 days under dark condition,
Incubation temperature is controlled to be 38 DEG C, humidity is 55%；At the 5th day of the incubation period, fertilization hatching egg is carried out60Co gamma Rays
2min, radiation dose rate is 15gy/min.At the 13rd day of the incubation period, fertilization hatching egg is carried out60Co gamma Rays 5min, spoke
Penetrating close rate is 15gy/min；
2) embryo egg after hatching freezes 36h at -20 DEG C；
3) embryo egg after freezing is shelled, smash to pieces, be dried, obtain final product.
Reference examples
1) the fertilization hatching egg of commercial generation Buddhist nun gram coral powder laying hen is sent in couveuse, hatch hatching 19 under dark condition
My god, control incubation temperature to be 38 DEG C, humidity is 55%；
2) embryo egg after hatching freezes 36h at -20 DEG C；
3) embryo egg after freezing is shelled, smash to pieces, be dried, obtain final product.
Each 10 pieces of the embryo egg of Example 1～6 and reference examples, shells, detects igy in the finished product that every piece of embryo egg is made one by one
Content, concrete outcome see table:
| Group | igy(mg/ml) |
| Matched group | 12.75±1.34 |
| Embodiment 1 | 30.08±0.56* |
| Embodiment 2 | 31.17±0.43* |
| Embodiment 3 | 29.66±0.28* |
| Embodiment 4 | 40.18±1.23* |
| Embodiment 5 | 39.02±1.07** |
| Embodiment 6 | 46.34±0.47** |
Note: compared with matched group, * p ＜ 0.05；* p ＜ 0.01.
For detecting the effect of the embryo egg enhance immunity of the application, immunity test is carried out to mice, Details as Follows:
1st, test method
The Embryo Gallus domesticus of embodiment 1～6 and reference examples are precooked, cooks, shell, rubbed, dried, pulverized and sieved plus life
Reason saline is configured to the embryo egg suspension that concentration is 1.2g/kg respectively.
Immunity test: 2 monthly age spf level km female white mice 56 are randomly divided into 7 groups, respectively test group 1～6 He
Matched group, every group 8.Every mice of test group 1～6 feeds the embryo egg that the embryo egg of 0.4ml embodiment 1～6 makes respectively and mixes
Suspension, the embryo egg of every mouse stomach 0.4ml reference examples of matched group makes embryo egg suspension.Mice free choice feeding, drinking-water, raise
During supporting, change water daily, the next day change bedding and padding.Daily gavage once, continuous gavage 30d.
2nd, preparation of reagents
Chicken red blood cell crbc) suspension: fresh anticoagulant Sanguis Gallus domesticus, put 2500r/min centrifugation 5min, abandon upper plasma and leukocyte
Layer, with 8 times of normal saline dilution erythrocyte, is gently mixed washing, inhales and abandon supernatant, and cyclic washing is colourless to supernatant, to remove
Remove the albuminous cell of plasma protein, normal saline, as packed red cells are abandoned in suction for the last time as far as possible, according to desired concn, use
Normal saline dilution chicken red blood cell.
Sheep red blood cell (SRBC) (srbc) suspension: sheep venous blood sampling, Sanguis caprae seu ovis are put in the sterilizing conical flask of bead towards one
The shake of individual direction, to take off fiber, puts into 4 DEG C of Refrigerator stores standby.
Complement: collection guinea pig blood, isolate serum (pooled serums of at least 5 Cavia porcelluss), 1ml hematocrit srbc is added to
In 5ml guinea pig serum, 30min placed by 4 DEG C of refrigerators, often vibrates, and supernatant, subpackage, -70 DEG C of preservations are gone in centrifugation.Used time is delayed with sa
Rush liquid to dilute by 1:10.
Hank ' s liquid: nacl 8g, kcl 0.4g, na2hpo4·12h2o 0.152g、kh2po40.06g, phenol red
(0.005g first uses nahco3Solution dissolves) it is settled to 1000ml with distilled water, use nahco3Solution adjusts ph value 7.2～7.4,
High temperature sterilize.
3rd, the mensure of index and spleen index
After off-test, mice fasting 12h weighs, and takes mouse spleen to weigh, is calculated as follows index and spleen index: index and spleen index
=spleen weight/body weight；
4th, the mensure of Phagocytosis By The Peritoneal Macrophages In Mice
The activation of mouse macrophage: before test, 4d gives every mouse peritoneal injection 2% hematocrit sheep red blood cell (SRBC) 0.2ml.
The incubation of macrophage: put to death mice with cervical dislocation, lumbar injection adds hank ' the s liquid of calf serum
4ml/ only, gently rubs abdominal part 20 times, fully to wash out peritoneal macrophage, then abdominal cavity is cut off an osculum, draw abdomen
Chamber washing liquid 0.5ml is mixed with 0.5ml 1%crbc suspension, takes 0.5ml mixed liquor, and less than in the agar circle of microscope slide, placement is incubated
Change incubation 15min in device.
Dyeing: non-attached cell is washed out rapidly after terminating by incubation with normal saline, fixes 1min in methanol solution,
Giemsa liquid dyes 15min, clean with distilled water flushing, dries, and is counted with 40 power microscopes, and be calculated as follows phagocytic rate and
Phagocytic index.
Number of macrophages/200 macrophage × 100% of phagocytic rate=phagocytosis chicken red blood cell
Chicken red blood cell sum/200 macrophages of phagocytic index=phagocytosis
5th, serum hemolysin (igm) measures
Immune animal: hematocrit srbc normal saline is made into 2% cell suspension, every mice pays off injection 0.2ml
Carry out immunity.Extracing eyeball after immune 7d takes blood to be placed in centrifuge tube, places about 1h, will solidify blood stasis tube wall and peel off, and so that serum is filled
Analyze, 2000r/min is centrifuged 10min, collect serum.Take serum 10 μ l, with 100 times of normal saline dilution.Dilute serum 1ml
With 0.5ml 5%crbc 0.5ml 10% complement mix, at 37 DEG C be incubated 30min, terminating reaction on ice-water bath.Centrifuging and taking
Supernatant, colorimetric at 540nm wavelength, using the blank of not increase serum as comparison.
6th, the impact to mice swimming time
After last gavage 30min, by the mice of root of the tail portion load 5% (body weight) sheet lead put swimming trunk (50cm × 50cm ×
40cm) went swimming, 25 DEG C ± 1.0 DEG C of water temperature, mice starts to death time, i.e. mice burden swimming time record from swimming
(min).
7th, result of the test
1) impact (x ± s) to mouse spleen index for the chicken embryo
Note: compared with matched group, * p ＜ 0.05；* p ＜ 0.01.
2) impact to Phagocytosis By The Peritoneal Macrophages In Mice
| Group | Phagocytic rate | Phagocytic index |
| Matched group | 43.50±3.70 | 0.64±0.07 |
| Test 1 group | 59.75±2.84* | 0.81±0.17* |
| Test 2 groups | 60.75±4.5** | 0.82±0.02* |
| Test 3 groups | 58.00±3.74* | 0.80±0.03* |
| Test 4 groups | 65.50±4.43* | 0.85±0.06** |
| Test 5 groups | 65.16±1.73* | 0.84±0.08* |
| Test 6 groups | 70.25±5.31** | 0.89±0.05* |
Note: compared with matched group, * p ＜ 0.05；* p ＜ 0.01.
3) impact to mice serum Hemolysin formation
Chicken red blood cell crbc) suspension: fresh anticoagulant Sanguis Gallus domesticus, put 2500r/min centrifugation 5min, abandon upper plasma and leukocyte
Layer, with 8 times of normal saline dilution erythrocyte, is gently mixed washing, inhales and abandon supernatant, and cyclic washing is colourless to supernatant, to remove
Remove the albuminous cell of plasma protein, normal saline, as packed red cells are abandoned in suction for the last time as far as possible, according to desired concn, use
Normal saline dilution chicken red blood cell.
To crbc immune mouse so as to lymphocyte produces specificity anti-crbc antibody, and discharge to peripheral blood, containing antibody
Serum when being incubated together with crbc, produce haemolysis under complement participates in and discharge hemoglobin, solution takes on a red color, measure molten
The absorption value of liquid, calculates half hemolysis value hc50, reflect igm antibody horizontal.
| Group | Half hemolysis value hc50 |
| Matched group | 428.91±36.43 |
| Test 1 group | 585.35±61.40* |
| Test 2 groups | 576.71±27.89* |
| Test 3 groups | 568.16±26.94* |
| Test 4 groups | 594.98±79.24** |
| Test 5 groups | 599.60±19.17* |
| Test 6 groups | 609.42±94.18* |
Note: compared with matched group, * p ＜ 0.05；* p ＜ 0.01.
4) impact to mice swimming time
| Group | Time (min) |
| Matched group | 7.67±0.35 |
| Test 1 group | 16.65±1.42 |
| Test 2 groups | 16.72±2.01 |
| Test 3 groups | 16.49±1.31 |
| Test 4 groups | 18.35±2.43 |
| Test 5 groups | 18.26±3.16 |
| Test 6 groups | 19.47±1.43 |
Note: compared with matched group, * p ＜ 0.05；* p ＜ 0.01.
For detecting the effect of the embryo egg resisting fatigue of the application, anti-fatigue test is carried out to mice, Details as Follows:
1st, test method
The Embryo Gallus domesticus of embodiment 1～6 and reference examples are precooked, cooks, shell, rubbed, dried, pulverized and sieved plus life
Reason saline is configured to the embryo egg suspension that concentration is 1.2g/kg respectively.
Spf level Kunming kind male white mouse 210, body weight 20g ± 2g, after white mice raises one week in advance, it is randomly divided into 7 groups,
Respectively test group 1～6 and matched group, every group 30.Every mice of test group 1～6 feeds 0.4ml embodiment 1～6 respectively
The embryo egg suspension made of embryo egg, the embryo egg of every mouse stomach 0.4ml reference examples of matched group makes embryo egg suspension.Little
Mus free choice feeding, drinking-water, in feeding process, change water daily, the next day change bedding and padding.Daily gavage once, continuous gavage 30d.
2nd, Loaned swimming test
After last gavage 30min, by the mice of root of the tail portion load 5% (body weight) sheet lead put swimming trunk (50cm × 50cm ×
40cm) went swimming, 25 DEG C ± 1.0 DEG C of water temperature, record mice starts to the 8s that swims like a tailor's goose not float from swimming, i.e. mice swimming time
(min).Start to exhaust the time to power by only recording motion, as mice swimming time, after mice power exhausts, pulled rapidly out water
Pond, dries up to put in cage with Blowing drum in couveuse and normally raises.
3rd, the mensure of blood urea nitrogen bun lactic acid dehydrogenase ldh
After last gavage 30min, not swimming with a load attached to the body 90min in the water of 30 DEG C ± 1.0 DEG C of water temperature, eye after rest 60min
Ball is taken a blood sample, and isolates serum, and automatic clinical chemistry analyzer measures serum urea nitrogen content.After last gavage 30min, eyeball is adopted
Blood, isolates serum, and automatic clinical chemistry analyzer measures lactic acid dehydrogenase activity.
4th, the mensure of hepatic glycogen hg
1) sample preparation
After mice last gavage 30min, in 30 DEG C ± 1.0 DEG C of water went swimming 90min of water temperature, put to death immediately, take liver,
Blotted with filtering residue after trial saline rinse, accurately weigh liver and get over 100mg, add 4mltca, often pipe homogenate 1min, will be homogenized
Liquid pours centrifuge tube into, is centrifuged 15min with 3000r/min, supernatant is moved in another test tube.Add tca in precipitation, even
Slurry 1min, then it is centrifuged 15min, remove supernatant, and merge with the supernatant of first time centrifugation, fully mix.
Take 1ml supernatant, put in 10ml centrifuge tube, often pipe adds 95% ethanol 4ml, fully mix between two kinds of liquid
Do not stay interface.With clean stopper beyond the Great Wall, vertically stand overnight under room temperature.After precipitation is complete, it is centrifuged in 3000r/min through test tube
15min.Carefully outwell supernatant and make test tube handstand place 10min.
Dissolve glycogen with 2ml distilled water, when adding water, the glycogen of tube wall is washed down.
Reagent blank: inhale 2ml distilled water to clean centrifuge tube.
Standard pipe: inhale 0.5ml glucose standard and 1.5ml distilled water is put in same pipe.
2) sample glycogen content measures
72%h2so4: add 280ml distilled water in beaker, add concentrated sulphuric acid 720ml.
Anthrone reagent: h2so4In put into 500mg anthrone, the highly purified thiourea of 10g, suitably rock beaker mix.After cooling
Put into refrigerator standby.
10ml anthrone reagent is added sample tube, fully mixes.From test tube injection anthrone reagent, test tube is placed on
Have a shower under cold water faucet.After all test tubes all reach cold water temperature, it is dipped in boiling water bath 15min, then moves on to psychrolusia,
It is cooled to room temperature.Liquid in pipe is moved into color comparison tube, with mensuration absorbance after the zeroing of reagent blank pipe under 620nm wavelength.Root
Mass conversion according to the liver being weighed becomes glycogen content.
3) glycogen content calculates
Glycogen content (mg/100g)=a in hepatic tissue1/a2×0.5×8/0.1×100×0.9；
A in formula1, a2: representative sample QC absorbance and standard pipe absorbance respectively；
Glucose content in 0.5:0.5ml glucose standard；
0.9: glucose is converted into the coefficient of glycogen；
8: extracting liquid volume, ml；
0.1: hepatic tissue grams, g.
6th, the mensure of blood lactase acid (ld)
After last gavage 30min, 2% (body weight) of bearing a heavy burden stops after the water went swimming 30min of 30 DEG C ± 1.0 DEG C of temperature,
After quiet 15min, eyeball blood sampling, isolates serum, in strict accordance with test kit, time-and-motion study absorbance is described, calculates blood lactase acid and contains
Amount.
6th, result of the test
1) impact to mice swimming time for the Embryo Gallus domesticus
| Group | Swimming time (min) |
| Matched group | 12.52±1.06 |
| Test 1 group | 25.30±3.07* |
| Test 2 groups | 23.08±2.43* |
| Test 3 groups | 26.52±3.50* |
| Test 4 groups | 30.42±2.50** |
| Test 5 groups | 31.95±6.60* |
| Test 6 groups | 35.15±2.82* |
Note: compared with matched group, * p ＜ 0.05；* p ＜ 0.01.
2) impact to mice blood urea nitrogen (bun), lactic acid dehydrogenase (ldh)
Body blood urea nitrogen content increases with the increase of work and sports load, and body is got over to load performance
Difference, blood urea nitrogen increase is more obvious.
| Group | bun(mmol/l) | ldh(u/l) |
| Matched group | 9.37±1.08 | 1877.67±134.03 |
| Test 1 group | 6.98±0.58* | 2302.33±147.00 |
| Test 2 groups | 6.89±0.95* | 2377.50±89.80* |
| Test 3 groups | 6.78±0.47* | 2351.33±250.86* |
| Test 4 groups | 6.45±0.68* | 2495.00±104.65* |
| Test 5 groups | 6.52±0.78** | 2405.00±89.10* |
| Test 6 groups | 6.11±0.78* | 2485.00±81.17* |
Note: compared with matched group, * p ＜ 0.05；* p ＜ 0.01.
3) impact to mice hepatic glycogen (hg) content
| Group | hg(mg/g) |
| Matched group | 6.58±0.55 |
| Test 1 group | 10.58±0.74* |
| Test 2 groups | 10.48±1.06** |
| Test 3 groups | 10.87±0.76* |
| Test 4 groups | 12.45±0.50* |
| Test 5 groups | 11.89±1.34** |
| Test 6 groups | 13.18±0.50* |
Note: compared with matched group, * p ＜ 0.05；* p ＜ 0.01.
4) impact to mice blood lactase acid (ld) content
The change of Serum lactic acid content illustrates glucolytic degree internal under anaerobic, is to evaluate resisting fatigue to commonly use
Biochemical indicator.
| Group | ld(mmol/l) |
| Matched group | 10.18±1.48 |
| Test 1 group | 7.98±0.77* |
| Test 2 groups | 7.56±1.00* |
| Test 3 groups | 7.73±0.66* |
| Test 4 groups | 6.59±0.84* |
| Test 5 groups | 6.20±0.45* |
| Test 6 groups | 6.01±0.22** |
Note: compared with matched group, * p ＜ 0.05；* p ＜ 0.01.
Claims (5)
1. improve anti-fatigue effect embryo egg health product processing technology it is characterised in that: comprise the following steps:
1) hatching egg of being fertilized is sent in couveuse, hatches 10～19 days under dark condition, in the incubation period, fertilization hatching egg is entered
OK60Co gamma Rays total time is 5～10min, and radiation dose rate is 12～20gy/min；
2) embryo egg after hatching freezes 24～36h at -20～-30 DEG C；
3) embryo egg after freezing is shelled, smash to pieces, be dried, obtain final product.
2. according to claim 1 improve anti-fatigue effect embryo egg health product processing technology it is characterised in that: incubating
The 4th～5 day of change phase, is carried out to fertilization hatching egg60Co gamma Rays 2～5min, radiation dose rate is 12～15gy/min.
3. according to claim 2 improve anti-fatigue effect embryo egg health product processing technology it is characterised in that: incubating
The 12nd～13 day of change phase, is carried out to fertilization hatching egg60Co gamma Rays 3～5min, radiation dose rate is 15～20gy/min.
4. according to claim 1 improve anti-fatigue effect embryo egg health product processing technology it is characterised in that: control
Incubation temperature is 37.5～38.5 DEG C, and humidity is 50～60%.
5. according to claim 1 improve anti-fatigue effect embryo egg health product processing technology it is characterised in that: fertilization
Hatching of breeding eggs 14～19 days.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107047458A (en) * | 2017-03-28 | 2017-08-18 | 枞阳县横山生态农业有限公司 | A kind of hatching method for lifting baby chick quality |
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