JP2017176171A - Eggs with accumulated fucoxanthin in form of fucoxanthinol and amaranthus xanthine a, functional processed foods, anti-obesity agent, lean body weight increasing agent and egg production method - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide eggs comprising a high level of fucoxanthin in a state in which the functionality thereof can be exhibited and a production method therefor.SOLUTION: Seaweed of 10 mass% to 20 mass% per 100 g of feed is blended therewith. The seaweed is preferably a brown algae such as Sargassum which comprises a lot of fucoxanthin. As a result eggs comprising a total of 40 μg or more of fucoxanthinol and amaranthus xanthine A, which has higher activity than fucoxanthinol, to 100 g of egg yolk are produced.SELECTED DRAWING: Figure 1

Description

  The present invention relates to a hen egg containing fucoxanthinol and amarosiaxanthin A, which are metabolites of fucoxanthin, which is a kind of carotenoid, and a functional processed food, an anti-obesity material, and a lean body mass increasing agent using the same. , And a method for producing chicken eggs.

  Per capita consumption of eggs in Japan is 329 a year as of 2013, the third highest in the world. The Japanese eat less than one egg per day, so it is a familiar food for the Japanese.

  In recent years, functional ingredients derived from it have been accumulated by increasing the content of ingredients and ingredients in the feed given to egg-collecting chickens, or by adding ingredients and ingredients that are not originally contained in eggs to the feed. Cases have been reported. Such a chicken egg is called a “functional egg”.

  One of the functional ingredients added to chicken eggs is fucoxanthin. Fucoxanthin is a kind of carotenoid contained in brown algae and the like. Fucoxanthin has been reported to have various functions such as anti-obesity action, anti-inflammatory action, and anti-cancer action, and has attracted attention in recent years. However, the fucoxanthin contained in brown algae etc. is very small, and the absorption metabolism of fucoxanthin varies from person to person. Therefore, it is necessary to have a fucoxanthin that can be absorbed quickly and obtain a high effect. .

  So far, for example, chickens are mixed with mixed feed and seaweed, fermented by shochu, ingested, fucoxanthin contained in seaweed is accumulated in the structure of fucoxanthinol in liver function, fucoxanthinol is A method of recovering from chicken liver and egg (see Patent Document 1) and a method of feeding a lipid extracted from seaweed to produce a chicken egg containing fucoxanthinol (see Non-Patent Document 1) have been reported. Yes. However, in the method described in Patent Document 1, a fermentation process by shochu is required, and in the method described in Non-Patent Document 1, lipids must be extracted from seaweed, both of which are troublesome. There was a problem.

  Non-Patent Document 2 reports that fucoxanthinol has a higher effect of inhibiting adipocyte differentiation in mouse fibroblast 3T3-L1 than fucoxanthin. Furthermore, Non-Patent Document 3 reports that the effect of suppressing adipocyte differentiation in mouse fibroblast 3T3-L1 is higher with amaroxacia xanthine A than with fucoxanthinol. From this, it is inferred that fucoxanthinol and amaroxaxanthin A have higher activity than fucoxanthin, and it is more preferable if they can be included in the form of amaroxacia xanthin A in chicken eggs. However, neither patent document 1 nor non-patent document 1 mentions amaroxaxanthin A.

JP 2014-68646 A

Noriyuki Iida, Masahiko Kitagawa, Satoshi Sugawara, Akiko Miyazaki Does the active ingredient of seaweed accumulate in chicken eggs? News from Kitamizu trial 77 22-25 (2009) Maeda H, Hosokawa M, Shimashima T, Takahashi N, Kawada T, Miyashita K. et al. Fucoxanthin and it's metabolites, fucoxanthinol, suppress adipocyte differentiation in 3T3-L1 cells. Int J Mol Med. 8 (1): 147-52 (2006) Yim MJ, Hosokawa M, Mizushina Y, Yoshida H, Saito Y, Miyashita K. et al. Suppressive effects of Amarociaxanthin A on 3T3-L1 adipocyte differentiation through down-regulation of PPAR gamma and C / EBPα mRNA expression. J Agric Food Chem. 59 (5): 1646-52 (2011)

  The present invention has been made on the basis of such a problem, and contains a chicken egg containing fucoxanthin in a state capable of exhibiting higher functionality, and a functional processed food, anti-obesity material, lean body weight using the same. An object is to provide an increasing agent and a method for producing chicken eggs.

  The hen's egg of this invention contains a total of 40 micrograms of fucoxanthinol and Amaroussiaxanthin A per 100g of egg yolk.

  The functional processed food, anti-obesity agent, or lean body weight gain agent of the present invention contains the egg of the present invention.

  The method for producing chicken eggs of the present invention is to produce chicken eggs containing fucoxanthinol and amaranthiaxanthin A by blending 10% by mass to 20% by mass of seaweed per 100g of feed and giving them to chickens. is there.

  According to the present invention, it contains amaroxaxanthin A having higher activity than fucoxanthinol, and the total content of fucoxanthinol and amaroxaciaxanthin A is higher than before, so that it has a higher function. Sex, for example, an anti-obesity function and a lean body mass gain function that increases body weight excluding fat can be obtained. In addition, fucoxanthin is accumulated in hen's eggs in the state of being metabolized to fucoxanthinol or amaroussiaxanthin A, so there is no need to metabolize in the body after eating the hen's eggs, and it is affected by the metabolic efficiency of individuals. Without absorption. Furthermore, chicken eggs are easy to eat and can be combined with various dishes.

  Therefore, if the hen's egg of this invention is used, the functional processed food, anti-obesity agent, or lean body weight gain agent which has higher functionality can be obtained. Moreover, since a predetermined amount of seaweed is blended in the feed and given to the chicken, the chicken egg of the present invention can be easily obtained.

It is a characteristic view which shows the relationship between the quantity of the seaweed in a feed, the period which gave the feed to the chicken, and the quantity of fucoxanthinol in egg yolk. It is a characteristic view which shows the relationship between the quantity of the seaweed in a feed, the period which gave the feed to the chicken, and the quantity of the amarosiaxanthin A in egg yolk. It is a characteristic view showing the weight fluctuation of a mouse. It is a characteristic view showing the organ weight of a mouse | mouth. It is a characteristic view showing the fat weight of a mouse | mouth. It is a characteristic view showing the plasma triglyceride density | concentration of a mouse | mouth.

  Hereinafter, embodiments of the present invention will be described in detail with reference to the drawings.

  The chicken egg which concerns on one embodiment of this invention contains a total of 40 micrograms or more of fucoxanthinol and amaroxaxanthin A per 100g of egg yolk. According to the egg production method described below, at least a higher content can be obtained. More preferably, it is 500 μg or more in total of fucoxanthinol and Amaroussia xanthin A per 100 g of egg yolk, and more preferably 2000 μg or more in total of fucoxanthinol and Amarousiaxanthin A per 100 g of egg yolk. Assuming that the mass of egg yolk in one chicken egg is about 20 g, if the total amount of fucoxanthinol and Amaroussiaxanthin A per 100 g of egg yolk is 500 μg or more, by eating 5 yolks per day, Xanthine can be ingested 500 μg per day, and if the total amount of fucoxanthinol and Amaroussia xanthin A per 100 g of egg yolk is 2,000 μg or more, one egg yolk is eaten per day, fucoxanthin 400 μg per day. If fucoxanthin is ingested to this extent, a high effect can be obtained.

  Further, the content of fucoxanthinol per 100 g of egg yolk is preferably 35 μg or more, more preferably 350 μg or more, and further preferably 1600 μg or more. The content of Amaranthiaxanthin A per 100 g of egg yolk is preferably 15 μg or more, more preferably 150 μg or more, and further preferably 400 μg or more.

  The chicken egg contains fucoxanthinol and amaroxaxanthin A, and thus has an anti-obesity function and a lean body mass increasing function for increasing body weight excluding fat. In general, if you want to reduce fat, you may lose weight, which may cause a decline in physical fitness, but according to this egg, in addition to anti-obesity function, increase lean body mass Because it has a function, it is beneficial for health promotion. In particular, a high effect can be obtained for elderly people whose physical strength tends to be lowered.

  Moreover, this hen's egg can be used for a functional processed food. Functional processed foods containing chicken eggs include various processed foods using chicken eggs, such as boiled eggs, egg baked eggs, omelets, egg tofu, steamed rice bowls, pudding, castella, cakes, cookies, etc. Sweets are listed. The functional processed food containing this hen egg has the same function as this hen egg, for example, and can be used as an anti-obesity functional processed food or a functional processed food for increasing lean body mass. Furthermore, this chicken egg can also be used as an anti-obesity agent containing this chicken egg or a lean body mass increasing agent containing this chicken egg.

  This chicken egg can be produced by, for example, blending 10% by mass or more and 20% by mass or less seaweed per 100g of feed and giving it to the chicken. That is, 10% by mass or more and 20% by mass or less of the entire feed is seaweed. If the amount of seaweed in the feed is less than 10% by mass, the content of fucoxanthinol and amaranthiaxanthin A in the egg yolk cannot be sufficiently increased, and the amount of seaweed in the feed is more than 20% by mass. If it is too large, the digestible energy in the feed will decrease, which is not preferable. A more preferable amount of seaweed is 15% by mass or more and 20% by mass or less per 100 g of feed. As the seaweed, for example, brown algae such as akamoku containing a large amount of fucoxanthin are preferable. The remaining 80% by mass to 90% by mass of the feed is constituted by, for example, a commercially available mixed feed.

  As described above, according to the present embodiment, it contains amaranthiaxanthin A having higher activity than fucoxanthinol, and the total content of fucoxanthinol and amaroxaciaxanthin A is higher than that of the conventional one. Therefore, it is possible to obtain a higher functionality, for example, an anti-obesity function and a lean body mass gain function that increases body weight excluding fat. In addition, fucoxanthin is accumulated in hen's eggs in the state of being metabolized to fucoxanthinol or amaroussiaxanthin A, so there is no need to metabolize in the body after eating the hen's eggs, and it is affected by the metabolic efficiency of individuals. Without absorption. Furthermore, chicken eggs are easy to eat and can be combined with various dishes.

  Therefore, if the hen's egg of this Embodiment is used, the functional processed food, anti-obesity agent, or lean body weight gain agent which has higher functionality can be obtained. In addition, since a predetermined amount of seaweed is blended in the feed and fed to the chicken, the chicken egg of the present embodiment can be easily obtained.

  The Example of this invention was shown, and also, as a result of testing the egg content of egg-laying hens bred with the feed of the present invention and the control feed (mixed feed), the content of fucoxanthin metabolites was tested, and The result of giving chicken eggs to mice and rearing them is shown.

Example 1
Julia chickens (27 weeks old), which are egg-laying hens, were raised for 28 days. “Control group” of only commercially available mixed feed, 90% by weight of blended feed with 10% by weight of akamoku powder in the blended feed “10% of akamoku” with 10% by weight of akamoku powder, and 20% by weight of akamok powder in the blended feed A total of 3 wards of “Akamok 20% ward” of 80% by weight of the mixed feed and 20% by weight of akamoku powder were provided. Multiple chickens were raised for each ward. The red powder was boiled and then finely chopped and dried to form a powder.

  The content of the three components of egg yolk fucoxanthin, fucoxanthinol, and amaranthiaxanthin A was quantified for each collected chicken egg every week. Extraction was performed by adding 8 mL of acetonitrile to 400 mg of egg yolk, shaking in a water bath at 56 ° C. for 20 minutes, and then centrifuging at 3000 rpm for 10 minutes to collect the supernatant. After repeating this operation twice, the solvent was removed by centrifugal concentration, and the sample dissolved in methanol was used as an analysis sample. Quantification was performed by HPLC (High Performance Liquid Chromatography). The measurement wavelength was 450 nm.

  The results of the test analysis are shown in Tables 1 and 2 and FIGS. Table 1 and FIG. 1 show the content of fucoxanthinol (unit: mg / 100 g) in 100 g of egg yolk. Table 2 and FIG. 2 show the content of amaranthiaxanthin A (unit: mg / 100 g in 100 g of egg yolk). ). The values shown in Tables 1 and 2 and FIGS. 1 and 2 are average values in each section. Fucoxanthinol and Amaroussia xanthin A were detected from the egg yolks of “Akamoku 10%” and “Akamoku 20%” one week after the start of the breeding. Although there were individual differences depending on the hen's egg, it was confirmed that fucoxanthinol and amaranthiaxanthin A were contained in a total of 40 μg or more even with the smallest content. Its content increases with the passage of the breeding period, and after 4 weeks of breeding, it should contain 1.60 mg of fucoxanthinol and 0.69 mg of amaranthiaxanthin A per 100 g of egg yolk as the average value of “Akamok 20%”. It was confirmed.

  In addition, the weights of the chickens in a total of 3 groups, the “control group”, “10% akamoku ward”, and “20% akamoku ward” were measured for each week. As a result, there was no significant change in body weight over 4 weeks in any group, and there was no significant difference between the groups. Furthermore, the average daily food intake was observed every week for the chickens in a total of 3 groups of the “control group”, “10% akamoku group”, and “20% akamoku group”. As a result, there was no significant difference between the “control group” and “Akamoku 10% group”, and there was no significant change in the average daily intake over 4 weeks. “Akamoku 20%” increased the average daily food intake after 3 weeks from the start of breeding compared to “control”.

  That is, if seaweed is blended in the feed within the range of 10% by mass or more and 20% by mass or less, the content of fucoxanthinol and amaranthiaxanthin A in egg yolk can be increased without reducing the weight of the chicken. I understood that I could do it.

(Example 2)
As an experimental group, a feed supplemented with egg yolk obtained in Example 1 in “Akamoku 10%” and “Akamoku 20%” (total of 500 μg or more of fucoxanthinol and Amaroussia xanthin A per 100 g of yolk) And a mouse (C57BL / 6J, male, 4 weeks old) was bred. In the feed, 20% of the chicken egg obtained in “Akamoku 20%” in Example 1 was added to a high-fat diet with 30% lipid. In addition, the hen's egg is what gave the feed which added the red moku continuously for 4 weeks or more. In addition, as a control group, a feed obtained by adding 20% of a chicken egg not added with akamoku (a chicken egg obtained in “control group” in Example 1) to a high-fat diet with 30% lipid was given, and the same as in the experimental group Mice were raised.

  In the breeding, 5 days of preliminary breeding was performed with AIN93G feed, and the experimental group and the control group were divided into groups so that there was no variation in body weight, and then in a plastic cage with sterile woodchip bedding. . The temperature in the breeding room was 23 ° C. ± 3 ° C., the humidity was 50 ± 3%, and the light / dark cycle was controlled at 12 hours. Feed and water were freely ingested and bred for 100 days. The body weight was continuously measured during the test breeding period. The measurement results are shown in FIG.

  After the feeding period, they were fasted for 12 hours. Subsequently, blood was collected by mixed anesthesia using a syringe containing heparin by heart determination, and the plasma triglyceride concentration (neutral fat concentration) was measured. Organs and fat were removed immediately after blood collection, washed well with physiological saline, and wiped off. The obtained results are shown in FIGS. FIG. 4 shows the organ weight, FIG. 5 shows the fat weight, and FIG. 6 shows the plasma triglyceride concentration.

  As shown in FIG. 3, the body weight was significantly higher in the experimental group compared to the control group. In addition, there was no difference in the amount of food intake and water consumption in the experimental group and the control group. Moreover, as shown in FIG. 4, the heart, liver, and spleen were significantly higher in the experimental group than in the control group. Furthermore, as shown in FIG. 5, compared with the control group, the experimental group showed a significantly lower fat weight around the testis and a lower total fat weight. From this, it was found that chicken eggs fed with a diet supplemented with akamoku had an effect of suppressing fat accumulation, that is, an anti-obesity function. In addition, the experimental group was found to have an effect of increasing body weight excluding fat, because the body weight was large despite the fact that the fat weight was lower than that of the control group. In addition, as shown in FIG. 6, the plasma triglyceride concentration was significantly lower in the experimental group than in the control group.

  Thus, according to the present invention, it is possible to produce a chicken egg containing fucoxanthinol or amaranthiaxanthin A, which has a higher effect than fucoxanthin originally contained in seaweed. Eggs containing fucoxanthinol and amaranthiaxanthin A of the present invention can be used as foods in the same way as normal eggs, and have the potential to be useful for health promotion including obesity suppression.

  Although the present invention has been described with reference to the embodiments and examples, the present invention is not limited to the above embodiments and examples, and various modifications can be made.

  It can be used for functional eggs and functional processed foods using the same.

Claims (7)

  A chicken egg comprising a total of 40 μg or more of fucoxanthinol and amarosiaxanthin A per 100 g of egg yolk.   The chicken egg according to claim 1, which has an anti-obesity function.   The egg according to claim 1, which has a function of increasing lean body weight by increasing body weight excluding fat.   The functional processed food characterized by including the hen egg of any one of Claims 1-3.   An antiobesity agent comprising the chicken egg according to claim 1.   A lean body weight increasing agent comprising the chicken egg according to claim 1 and having a lean body weight increasing function for increasing body weight excluding fat.   A method for producing a chicken egg containing fucoxanthinol and Amaroussiaxanthin A by blending 10% by mass or more and 20% by mass or less seaweed per 100g of feed and giving the chicken.

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