CN1248596C - Method for preparing gamma-amino butyric acid rich type rice germ flour, and its application - Google Patents
Method for preparing gamma-amino butyric acid rich type rice germ flour, and its application Download PDFInfo
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- CN1248596C CN1248596C CNB200410014287XA CN200410014287A CN1248596C CN 1248596 C CN1248596 C CN 1248596C CN B200410014287X A CNB200410014287X A CN B200410014287XA CN 200410014287 A CN200410014287 A CN 200410014287A CN 1248596 C CN1248596 C CN 1248596C
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Abstract
The present invention relates to a method for preparing gamma-amino butyric acid rich type rice germ flour, and application, and belongs to the technical field for finely processing rice. The present invention provides an endogenous enzyme technology, or a technology for preparing rice protein hydrolysate and rice germ by additional proteinase, or a technology of direct conversion of rice and sodium glutamate. The three technologies respectively enrich gamma-amino butyric acid (GABA). Through the three technologies, rice germ flour products which contain rich GABA are prepared. The technology of the present invention is simple and convenient, the effect for enriching GABA is obvious, and the present invention establishes a solid foundation for the utilization of rice ger. The product can be used as health food which has the function for resisting fatigue. The added value of the utilization of rice germ is greatly improved. The economic benefits and the social benefits are high.
Description
Technical field
A kind of preparation method and application of being rich in GABA rice germ flour belongs to rice intensive processing technical field.
Background technology
The rice embryo is the strongest part of seed physiologically active as the primary tissue and the separate living tissue of cereal kernel, accounts for the 2-2.2% of paddy by weight, and the 2.6-2.9% of brown rice is the byproduct of cereal processing.During most of plumule comes off accessory substance rice selenium and rice bran in the rice process, in the rice process, all the rice plumule is not put forward to be used at present, cause the waste of high-quality resource.
As the rice plumule of rice elite, nutrition is very abundant, and its protein and lipid content are all more than 20%, and amino acid is formed comparatively balance in the protein, and wherein the essential amino acid ratio meets the nutrition Reasonable Mode of FAO/WHO suggestion.Therefore rice germ protein matter is a kind of good protein that belongs to full price, and its quality can compare favourably with egg.Content of vitamin E is up to 200～300mg/100g in the rice plumule lipid, and 70% above aliphatic acid is unrighted acid.The experimental results shows that it is synthetic that the fat in the rice plumule can suppress cholesterol, anti-hemostatic tube cholesterol deposition and prevention diabetes; Wherein the vitamin E than horn of plenty can prevent cutaneous pigmentation and delaying decrepitude of skin.In addition, also contain abundant trace element and mineral matter in the rice germ.
GABA (GABA) is the inhibiting nerve transmitter substance that exists in mammal brain, the spinal cord, by glutamic acid through glutamate decarboxylase (GAD) catalyzed conversion.Studies show that that GABA has is hypotensive, improve the brain function, ataraxy, promotion long-term memory, promote effects such as growth hormone secretion, renal function activation, liver function activation, is applied to will be well worth doing in the functional food.In recent years, be rich in the research and development of GABA food, comprise the GABA food of plant (rice plumule, rice bran, green tea, pumpkin etc.), microorganism origin such as (lactic acid bacteria, yeast), become abroad, the focus of Japanology especially.
The glutamic acid decarboxylase activity is higher in the rice germ, is suitable as very much the functional food that GABA is rich in preparation.GABA has significant inhibitory effect to blood pressure, and the effect that improves kidney, liver function is arranged.Glutamic acid in the plumule converts GABA under the effect of enzyme.Usually GABA contains 20-50mg/100g approximately in the rice plumule; after this handles, increase 7 times approximately, can reach 300-400mg/100g, be rich in that GABA rice plumule can be made particle or tablet is made nutrition subsidy food; also can add cake, bread, powder soup material, Noodles, dairy products etc. to.Estimate that China's rice germ annual production is more than 100,000 tons, the therefore development and use of rice plumule, increment conversion are highly significant.
Summary of the invention
The purpose of this invention is to provide a kind of preparation method and application of being rich in GABA rice germ flour, can the production high added value have a health care be rich in GABA rice germ flour healthy food, give full play to the trophic function and the effect of meter plumule.
Technical scheme of the present invention: with endogenous enzyme process or add protease and make that a meter embryo protein hydrolyzate adds meter plumule or a rice embryo directly transforms three kinds of technologies of sodium glutamate and prepares a meter embryo GABA pregnant solution respectively, to the gained pregnant solution through colloid mill is pulverized, spray-drying obtains being rich in GABA rice germ flour.
Three kinds of technologies to the GABA in the rice germ flour (GABA) carries out enrichment are described below
1. the endogenous enzymes legal system is equipped with rice embryo GABA pregnant solution
Take by weighing a certain amount of meter plumule, the water that adds several times, under certain pH and temperature, vibrate, make the endogenous protease decomposing protein in meter embryo generate free glutamic acid, free glutamic acid generates GABA under the effect of rice embryo GAD, question response filters after a period of time, gets filtrate 10 μ l paper chromatographies and measures alpha-aminobutyric acid content.Through optimizing, the GABA process conditions are controlled to be in the endogenous enzymes enrichment rice plumule: 40 ℃ of temperature, material-water ratio are 1/7, pH5.6,7 hours time.Add CaCl
2500-700 μ mol/L, the GABA enriching quantity can improve 30%.
The amount of GABA can be brought up to about 6.15mg/g by the 0.28mg/g before the enrichment not in the rice germ flour that is rich in GABA that makes through post processing, has increased by 21 times.Though GABA content only is 0.88mg/ml in the rice embryo pregnant solution, the utilization rate of Glu also has only 22.4% in the rice material plumule.But, utilize the technology of GABA in the endogenous enzymes enrichment rice plumule simple, with low cost, for the GABA concentration requirement not high contain the GABA health food, be fit closely as drinks etc.
The rice embryo powder of table 1 endogenous enzymes enrichment GABA is formed
| Composition | Content (g/100g) |
| GABA fat protein total reducing sugar crude fibre moisture ash content | 0.615 22.3 21.8 30.3 12.3 4.3 6.1 |
2. adding protease makes a meter embryo protein hydrolyzate and adds a meter plumule preparation rice embryo GABA pregnant solution
(a) add trypsase and be hydrolyzed, the hydrolysis process condition is controlled to be: material-water ratio 1/14, pH8.0,45 ℃ of temperature, 7 hours time, enzyme concentration 4080 unit of activity/g rice plumule;
Under this optimum process condition, the output of Glu can reach 2.35mg/ml in the rice embryo protein hydrolyzate, the percent hydrolysis of Glu (free Glu accounts for the percentage of raw material Glu after the hydrolysis) reaches 85%, for enrichment GABA provides more Glu, has also improved utilization ratio of raw materials greatly.
(b) the enzyme condition of living of going out is controlled to be: 80 ℃ of temperature, and 15min, after the cooling, the amount that adds the rice plumule was 1.5g/100ml, in 40 ℃ of reactions 6 hours.
The rice plumule that adds 1.5g/100ml, 40 ℃ are continued reaction 6h, and GABA output can reach 1.9mg/mL, be equivalent to 2.26g/100g rice plumule, improve 3.7 times than endogenous enzyme enrichment GABA, therefore utilized to add trypsin hydrolysis rice embryo protein enrichment GABA, not only improved utilization ratio of raw materials; Also improve the concentration of GABA in the output of GABA and the pregnant solution significantly, the preparation that makes it to be suitable for more being rich in GABA rice plumule healthy food.
The rice embryo powder of table 2 exogenous protease enrichment GABA is formed
| Composition | Content (g/100g) |
| GABA fat protein total reducing sugar free amino acid crude fibre moisture ash content | 2.3 23.0 5.8 29.1 16.0 12.1 4.5 6.1 |
3. the rice embryo directly transforms sodium glutamate preparation rice embryo GABA pregnant solution, and conversion condition is controlled to be: the phosphate buffer of rice embryo and 0.08mol/L, pH5.6, solid-liquid ratio 1/10, the addition of phosphopyridoxal pyridoxal phosphate are the 2mmol/mol sodium glutamate, CaCl
2Addition be the 20mmol/mol sodium glutamate, a rice embryo addition is the 500g/mol sodium glutamate, 40 ℃ of temperature were reacted 6 hours.
Under these conditions, the conversion ratio of sodium glutamate can reach 100%, GABA content in the solution is 20.7mg/ml at this moment, be equivalent to every 100g rice plumule and produce GABA20.7g, method than preceding two kinds of rice embryo enrichment GABA has improved 33.6 and 9 times respectively, and do not have sodium glutamate residual, can in difference in functionality food, add and use.
Table 3 sodium glutamate directly transforms the rice germ flour of enrichment GABA and forms
| Composition | Content (g/100g) |
| GABA fat protein total reducing sugar crude fibre moisture ash content | 20.6 16.3 15.8 28.3 8.3 4.3 5.0 |
The rice embryo GABA pregnant solution that above-mentioned three kinds of technologies are prepared is pulverized through colloid mill, and spray-drying obtains being rich in the rice germ flour of GABA.
Be rich in the application of GABA rice germ flour, can be used for the health food of anti-fatigue effect.
Beneficial effect of the present invention: the rice plumule can be used as a kind of nutritious, healthy food raw material that physiological function is remarkable and further development and use, so not only can improve the economic benefit of rice processing comprehensive utilization value and grain-production, also can make grain-production go on eucyclic road.Be rich in the research and development of GABA rice plumule healthy food, belong to initiative work at home.The present invention uses modern biotechnology that the technology of GABA in the enrichment rice plumule is provided, and the rice germ flour that is rich in GABA for preparing has anti-fatigue effect.
The specific embodiment
Embodiment 1
Take by weighing the rice plumule of 15kg, add the water of 105kg, add 7gCaCl
2, oscillating reactions is 7 hours under pH5.6 and 40 ℃ of temperature.The reactant colloid mill is pulverized, and spray-drying obtains being rich in the rice germ flour of GABA.
Embodiment 2
Take by weighing the rice plumule of 15kg, add the water of 210kg, add the trypsase of 1-3kg, 45 ℃, pH8.0 reacted 7 hours.Behind 80 ℃ of enzyme 15min that go out of temperature, be cooled to 40 ℃, add 3kg rice plumule again, continue reaction 6 hours, the reactant colloid mill is pulverized, spray-drying obtains being rich in the rice germ flour of GABA.
Embodiment 3
Take by weighing the rice plumule of 15kg, add the phosphate buffer of 150kg0.08mol/L, pH5.6, add the 5kg sodium glutamate, add the 16g phosphopyridoxal pyridoxal phosphate, add 67gCaCl
2, 40 ℃ of temperature were reacted 6 hours, and the reactant colloid mill is pulverized, and spray-drying obtains being rich in the rice germ flour of GABA.
Claims (1)
1. preparation method who is rich in GABA rice germ flour is characterized in that:
(a) preparation rice embryo GABA pregnant solution:
Add trypsase and be hydrolyzed, the hydrolysis process condition is controlled to be: material-water ratio 1/14,45 ℃ of temperature, 7 hours time, pH8.0, enzyme concentration 4080 unit of activity/g rice plumule; The enzyme that the goes out condition of living is controlled to be: 80 ℃ of temperature, and 15min, after the cooling, the amount that adds the rice plumule was 1.5g/100ml, in 40 ℃ of reactions 6 hours;
(b) gained pregnant solution is pulverized through colloid mill, and spray-drying obtains being rich in the rice germ flour of GABA.
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| Application Number | Priority Date | Filing Date | Title |
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| CNB200410014287XA CN1248596C (en) | 2004-03-10 | 2004-03-10 | Method for preparing gamma-amino butyric acid rich type rice germ flour, and its application |
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| CNB200410014287XA CN1248596C (en) | 2004-03-10 | 2004-03-10 | Method for preparing gamma-amino butyric acid rich type rice germ flour, and its application |
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| CN1248596C true CN1248596C (en) | 2006-04-05 |
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100415119C (en) * | 2005-10-13 | 2008-09-03 | 南京农业大学 | Method for producing gamma-aminobutyric acid enriched high milled rice, and product thereof |
| CN101352216B (en) * | 2008-09-04 | 2011-12-28 | 华中农业大学 | Rice product rich in gamma-aminobutyric acid and method for producing the same |
| CN101810322B (en) * | 2009-02-20 | 2012-11-14 | 南京工业大学 | Method for producing food rich in gamma-aminobutyric acid |
| CN102160627A (en) * | 2011-03-22 | 2011-08-24 | 西藏月王生物技术有限公司 | Highland barley with rich gamma-aminobutyric acid and preparation method thereof |
| CN102793171A (en) * | 2012-08-29 | 2012-11-28 | 陕西科技大学 | Method of increasing gamma-aminobutyric acid in buckwheat tea |
| CN106962273B (en) * | 2017-03-28 | 2021-06-22 | 枞阳县横山生态农业有限公司 | Embryo egg hatching method for improving production characteristics of hens |
| CN108719764A (en) * | 2018-05-23 | 2018-11-02 | 广东医科大学 | A kind of production method of compound bio enzyme process high-content GABA functionality rice flour |
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Assignee: Sufu Biological Technology Development Co., Ltd., Xuzhou Assignor: Jiangnan University Contract fulfillment period: 2009.2.4 to 2011.12.30 contract change Contract record no.: 2009320000317 Denomination of invention: Method for preparing gamma-amino butyric acid rich type rice germ flour, and its application Granted publication date: 20060405 License type: Exclusive license Record date: 2009.3.10 |
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