CN104397321B - A kind of method that soybean protein depth growth prepares compound amino acid - Google Patents
A kind of method that soybean protein depth growth prepares compound amino acid Download PDFInfo
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- CN104397321B CN104397321B CN201410742166.0A CN201410742166A CN104397321B CN 104397321 B CN104397321 B CN 104397321B CN 201410742166 A CN201410742166 A CN 201410742166A CN 104397321 B CN104397321 B CN 104397321B
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Abstract
The present invention discloses a kind of method that soybean protein depth growth prepares compound amino acid.The present invention will add the soybean protein isolate extrusion process after water by the expanded extruder of screw rod, and the soybean protein of extrusion is crushed；The soyabean protein powder after crushing is formulated as soy bean proteinous soln with water, disperseed and aquation；It is warming up to 45~55 DEG C, pH value is adjusted to 8~9, add alkali protease, it is 7~7.5 to be digested in 45~55 DEG C of constant temperature stirrings to system pHs, add neutral proteinase and flavor protease, open two-stage ultrafiltering system and carry out enzyme membrane reaction, the trapped fluid of first order milipore filter and the trapped fluid of second level milipore filter are pumped back to retort reuse；When the volume of reaction substrate reduces 1/4, the water of equal volume amounts is supplemented；The permeate of second level milipore filter is concentrated, sterilized, is spray-dried, and obtains composite aminophenol powder.This method yield is high, and free amino acid accounts for total amino acid content more than 40% in obtained compound amino acid.
Description
Technical field
The invention belongs to protein deep processing and utilization technical field, and in particular to a kind of soybean protein depth growth prepares multiple
The method for closing amino acid.
Background technology
Amino acid is to constitute biological body protein and the most basic material relevant with vital movement, is to constitute protein
Base unit, has close relationship with biological vital movement, and it has special physiological function in antibody, is organism
One of internal indispensable nutritional ingredient.Constituting the amino acid of human body has kind more than 20, is divided into essential amino acid and nonessential ammonia
Base acid two types.Wherein " nonessential amino acid " people physical efficiency itself is synthesized, and is supplemented without food or medicine, " essential amino acid "
(including isoleucine, leucine, lysine, methionine, tryptophan, threonine, phenylalanine and valine) human body itself is not
It can synthesize or aggregate velocity can not meet somagenic need, it is necessary to be supplemented from food or medicine.Human body lacks any
Essential amino acid, can cause physiological function abnormal, influence being normally carried out for organism metabolism, the different sub-health status of generation or disease
Disease；Equally, if human body lacks some nonessential amino acid, organism metabolism obstacle can also be produced.Research finds that amino acid has many
Various physiologically active is planted, such as lysine, which has, to be promoted human development, strengthens the work of immune raising central nervous tissue function
With tryptophan can improve sleep, improve energy, prevent depressive emotion, and arginine has protection liver, preventing and treating renal failure, improves and exempt from
Epidemic disease systemic-function, glutamic acid can treat hepatic coma, improve children's intelligence development, and leucine has regulation blood sugar level, promoted
Muscle recovery, treatment dizziness and neurological disorder effect after training, asparatate have reinforcing nutrition and immunity of organism
Function, set up, protect cardiac muscle, treatment heart disease and diabetes, suppress cancer cell multiplication the effects such as.It can be seen that, amino acid is
The material base of vital metabolic, without amino acid just without life.
In recent years, in the market emerges in large numbers several amino acids health food, such as auspicious year amino acid tablet, the western amino acids oral-liquor of ring,
Raw compound amino acid soft capsule of triumphant a large bell compound amino acid oral liquid, China Resources magic power aminoacid functional beverage, Sheng etc., these products can
Effectively supplement human nutrition, improvement sub-health state, promotion function lifting, popular with consumers again.Using amino acid to be main former
Expecting the food (containing functional food) of exploitation has wide market prospects.
At present, on the market food-grade amino acid powder is more with vegetable raw material processed side product (dregs of beans, peanut meal, corn
The dregs of rice, wheat gluten etc.) it is raw material, after sour hydrolysis process, then neutralized, refined, spray drying is processed, i.e. hydrolysis is planted
Thing albumen (HVP, Hydrolyzed vegetable protein).This method conventional process is：Using 3.6M hydrochloric acid 100~
125 DEG C hydrolyze 4~24 hours, add sodium carbonate or sodium hydroxide is neutralized, then filtered again with charcoal absorption；It is characterized in water
Solution is rapid thorough, and most of protein hydrolyzes l-shaped amino acid, and product free amino acid, salt content are high；It has the disadvantage that strong acid is made
With strong reaction, sensitive amino acid (such as asparagine, glutamine, cystine, tryptophan, serine, threonine) easily quilt
Destruction, can limit it in food, particularly luxury food because remaining grease generation chloropropyl alcohol class carcinogenicity material in raw material
Application.
Enzymatic hydrolysis has that mild condition, side reaction are few, sensitive amino acid is not destroyed, the spy that hydrolysis degree is easily controlled
Point, particularly without using hydrochloric acid, do not generate carcinogenic substance chloropropyl alcohol class material, nutritive effect composition and retain perfect square face and have not
Analogous advantage, is increasingly paid attention to both at home and abroad.Enzymatic hydrolysis temperature is generally between 45~60 DEG C, and the reaction time is then
3~48 hours, hydrolysate was in addition to containing amino acid, also containing a large amount of small-molecular peptides.The shortcoming of enzymatic hydrolysis is mainly：
(1) enzyme preparation costly, the enzymolysis time it is long, production cost is higher；(2) because enzyme has stronger selectivity, albumen
The tertiary structure of matter, amino acid sequence and enzymolysis condition (including the species and vigor of enzyme, pH value, concentration of substrate, product
Concentration, activator, inhibitor, hydrolysis temperature, hydrolysis time etc.) there is considerable influence to enzymolysis, hydrolysis degree is relatively low.Just
It is that, because above reason, enzymatic hydrolysis is mainly used in the animal protein raw material easily digested relatively, such as meat, sea both at home and abroad at present
Products material, product is mainly used in meat product processing, instant noodles, dilated food and flavouring (soy sauce, oyster because fresh fragrance is protruded
Oil, baste, tartar sauce, chickens' extract, chicken powder etc.) in；In recent years, silkworm chrysalis enzymolyzing prepares amino acid powder technology and achieves breakthrough, silkworm
Pupa composite aminophenol powder has certain application in food (including luxury food, such as health food), but because of the distinctive gas of silkworm chrysalis
Taste, limits its extensive use.Food is met using the composite aminophenol powder of the new high-quality of all kinds of development of raw materials of enzymatic hydrolysis
The development of processing industry has great importance and good market prospects.
China is large agricultural country, plant protein material aboundresources.Peanut, wheat, the yield of corn and soybean occupy generation respectively
Boundary first, one, two, four, 2013 be respectively 1.22 hundred million tons, 17,000,000 tons, 2.11 hundred million tons, 12,000,000 tons.Using enzyme process water
Solution vegetable protein exploitation high-quality composite aminophenol powder is especially suitable for China's national situation, and plant protein material source is naturally also complied with
The trend of food processing industry development, this is for promoting agricultural byproducts intensive processing, promoting industry restructuring significant.
However, vegetable protein molecule is acted on due to hydrogen bond, hydrophobic bond, disulfide bond etc., close structure and have stronger hydrophobicity, be difficult by
Protease hydrolytic.Not yet there is the vegetable protein sources compounded amino acid product of enzymatic hydrolysis on domestic market.
The content of the invention
In order to solve the difficult point and weak point of above-mentioned prior art, primary and foremost purpose of the invention is to provide a kind of soybean
The method that albumen depth growth prepares compound amino acid.It is quick that the preparation method improves hydrolysate of soybean protein using extrusion processing
Perception, then enzyme mebrane reactor technology is combined by complex enzyme combination enzymolysis, preparing high content, (free amino acid accounts for total amino acid
Content more than 40%) compounded amino acid product, production process is easy to control, is adapted to industrialized production.
It is another object of the present invention to provide the compound amino acid obtained by above-mentioned preparation method.The compounded amino
Acid source is natural, and amino acid classes are complete, ratio is appropriate, and the wherein proportion of composing of essential amino acid is close to FAO/WHO ideal moulds
Formula, digests and absorbs rate height, can be used as the raw material of varieties of food items, particularly health food.
The present invention is realized by following technical proposals：A kind of soybean protein depth growth prepares compound amino acid
Method, comprises the following steps：
(1) raw material moisture is adjusted：Water is added in soybean protein isolate raw material, is stirred, aqueous soybean protein is obtained；
(2) extruding pretreatment：With the expanded extruder of screw rod by aqueous soybean protein extrusion process, after extruding terminates, then will
The soybean protein of extrusion is crushed；
(3) the scattered and aquation of soybean protein：The soyabean protein powder after crushing is formulated as soy bean proteinous soln with water, point
Dissipate and aquation；Then 45~55 DEG C are warming up to, regulation pH value obtains reaction substrate to 8~9；
(4) the soybean protein enzyme membrane reaction based on ultra-filtration and separation：Alkaline egg is added in the reaction substrate that step (3) is obtained
White enzyme, it is 7~7.5 to be digested in 45~55 DEG C of constant temperature stirrings to system pHs；Neutral proteinase and flavor protease are subsequently added into,
Open two-stage ultrafiltering system and carry out enzyme membrane reaction, the trapped fluid of first order milipore filter and the trapped fluid of second level milipore filter are pumped back to
Retort is reused；When the volume of reaction substrate reduces 1/4, the water of equal volume amounts is supplemented；Kept in whole course of reaction
Constant temperature stirring；
(5) concentrate, sterilize, dry：The permeate of second level milipore filter is concentrated, obtained concentrate sterilization, spraying
Dry, obtain composite aminophenol powder.
Raw material described in step (1) refers to as defined in GB/T 20371-2006 in terms of butt, protein quality percentage contains
The soybean protein isolate of the soybean protein isolate, preferably low ash content of amount >=90%, such as in terms of butt, content of ashes content≤
7.0% soybean protein isolate；
The water content in aqueous soybean protein described in step (1) is preferably mass percent 20~30%；
Single screw rod extruding or twin-screw extruder, preferably twin-screw extruder may be selected in extruding pretreatment described in step (2)；
The parameter of described extruding pretreatment is preferably：95~105 DEG C of extrusion temperature, 15~25mm of nib aperture, screw rod
100~120r/min of rotating speed；
The degree of crushing described in step (2) is preferably that the soybean protein of the extrusion is crushed into 20~100 mesh, excellent
Select 40~80 mesh；
The content of soyabean protein powder is mass percent 5~10% in soy bean proteinous soln described in step (3), preferably
For 6~8%；
The condition of scattered and aquation described in step (3) is preferably：Shear rate is 2000~3000rpm, and the time is
10~20min；
PH value described in step (3) is preferably 8~8.5；
PH value described in step (3) is adjusted by adding aqueous slkali；
Described aqueous slkali is preferably sodium hydroxide solution；
The addition of alkali protease described in step (4) is the 1~2% of soybean protein isolate material quality；Step
(4) addition of the neutral proteinase described in is the 0.5~1% of soybean protein isolate material quality；
The addition of flavor protease described in step (4) is the 1~2% of soybean protein isolate material quality；
The rotating speed of stirring described in step (4) is preferably 20~80rpm, more preferably 20~60rpm；
The molecular cut off of first order milipore filter described in step (4) be 5000Dal, membrane filtration pressure be 0.1~
0.5MPa；
The molecular cut off of second level milipore filter described in step (4) be 1000Dal, membrane filtration pressure be 0.1~
0.5MPa；
The time of enzyme membrane reaction described in step (4) is preferably 4~8h；
The degree of concentration described in step (5) is that solid content reaches weight/mass percentage composition 20~40%, preferably 25
~35%；
The mode of sterilization described in step (5) is preferably ultrahigh-temperature instant sterilizing (UHT)；
Spray drying described in step (5) refers to dry by spray drying tower, and operating parameter is preferably inlet temperature
For 160~180 DEG C, outlet temperature is 70~90 DEG C；
Described water is preferably deionized water or distilled water.
A kind of compound amino acid, is prepared by the above method；Its free amino acid accounts for total amino acid content more than matter
Measure percentage 40%, preferably mass percent 46~49%.
The present invention compared with prior art, has the following advantages that and beneficial effect：
(1) present invention uses expelling-expansion pretreatment soybean protein, has been obviously improved the enzymolysis sensitiveness of soybean protein, promotees
The efficient development of follow-up enzyme digestion reaction is entered, has improved the palliating degradation degree of soybean protein, so as to improve the yield of free amino acid.
Soybean protein molecule has close stereochemical structure, and due to the effect such as hydrogen bond, hydrophobic bond, disulfide bond, peptide chain is curled in albumen point
Sub internal to be similar to spherical, restriction enzyme site is contained inside protein molecule, it is difficult to by protease hydrolytic, it is necessary to it is carried out pre-
Processing, changing its structure makes enzyme effect point fully expose to improve follow-up enzymolysis efficiency.But the control of pretreatment parameter is on the one hand
Must be suitable, it is impossible to excessively, enzyme otherwise can be hindered on the contrary to the hydrolysis of soybean protein；On the other hand, be according to follow-up work
Selected with enzyme viability, with the best of breed effect for reaching pretreatment and subsequently digesting.Traditional heat treatment can be a small amount of
Increase degree of hydrolysis, polypeptide yield and the free amino acid yield of enzyme digestion reaction, but DeGrain；And extrusion is high using it
Temperature, high pressure, high shear, can be sufficiently destroyed the original fine and close space structure of soybean protein, the parameter of extrusion processing
Selection is particularly important, low material moisture, low swelling temperature, short nib, screw speed soon to the destructiveness of protein structure not
It is enough, on the contrary, during the slow extrusion of high material moisture, high temperature, long nib, screw speed, the friendship inside protein molecule can be aggravated
Connection.We are bright according to effect enzyme viability, controlled by the science to extrusion expansion parameter, obtain the ideal of soybean protein structure
Destructiveness, optimal substrate specificity is provided for the depth growth of soybean protein.
(2) present invention prepares high content compound amino acid by continuous enzyme membrane reaction depth enzymatic hydrolysis of soybean albumen, using super
Filter technology and enzyme digestion reaction coupling, the yield and quality of compounded amino acid product are lifted by the control of following 4 key links：
1. the selection of enzyme：Because endopeptidase cuts the peptide bond generation less polypeptide of molecular weight inside protein or polypeptide, and exopeptidase
Amino, the carboxyl terminal hydrolysising peptide key of (including aminopeptidase, carboxypeptidase) then from protein or peptide molecule, dissociate amino acid,
The present invention is using alkali protease, neutral proteinase and flavor protease complex enzyme hydrolysis, both in view of the appropriate effect bar of each enzyme
Part and combined efficiency, it is also considered that to the single-minded sex differernce of each enzyme hydrolysis amino acid chain, to ensure cooperative synergism effect, so as to obtain
The free amino acid of high yield pulp1；2. the selection of ultrafiltration system：To be reduced as far as free amino acid, small peptide generation to digesting
The suppression of journey, improves free aminoacid content to greatest extent, is reacted using continuous enzyme membrane, on the one hand to ensure free amino acid
And small peptide is efficiently separated with reaction system, the operating efficiency of ultrafiltration system is on the other hand considered, the present invention is according to soybean egg
The feature of white enzymolysis product uses two-stage ultrafiltering system, and the molecular cut off of first order milipore filter is 5000Dal, second level ultrafiltration
The molecular cut off of film is 1000Dal, and two-stage ultrafiltering membrane flux is matched by adjusting pressure in course of reaction, continuous to ensure
Enzyme membrane reaction efficiency；3. enzymolysis pH control：Because excessive addition acid, alkali can be entered by Ultra filtration membrane in enzyme digestion reaction
Into the permeate of second level milipore filter, difficult, the purity and quality of influence enzymolysis product compound amino acid are further separated,
One aspect of the present invention improves alkali protease functioning efficiency by the control of initial pH value, on the other hand timely by ultrafiltration system
Free amino acid is isolated on ground and small-molecular peptides slow down the fluctuation of pH value of reaction system, and the optimum choice of desmoenzyme combination ensures body
What system reacted continues that the pH value that acid, alkali adjust reaction system need not be added in efficiently progress, course of reaction；4. reaction system bottom
The control of thing concentration：The progress reacted with enzyme membrane, the solid content and viscosity of trapped fluid are significantly increased, and the film of milipore filter leads to
Amount declines, and supplement equivalent water is to ensure whole when the present invention reduces 1/4 by the optimum choice of initial substrate concentration, substrate volume
Concentration of substrate is conducive to the progress that enzyme membrane reacts in individual course of reaction.
(3) the enzymolysis product free amino acid obtained using the present invention is accounted for total amino acid content and is up to more than 40%, remaining
For small-molecular peptides, product safety, physiologically active are good, can be widely applied to varieties of food items, especially functional food, production process temperature
With, it is easy to control, to production equipment require it is low, it is easy to continuous operation, with the advantage that acid-hydrolysis method is incomparable.
Brief description of the drawings
Fig. 1 is the flow chart for the method that the soybean protein depth growth that embodiment 1 is provided prepares compound amino acid.
Embodiment
With reference to embodiment and accompanying drawing, the present invention is described in further detail, but embodiments of the present invention are not limited
In this.
Embodiment 1
Operating process is as shown in Figure 1：
(1) raw material moisture is adjusted：In 100kg soybean protein isolates raw material, (in terms of butt, 92.6%) protein content is
Middle addition deionized water, dispersed with stirring is uniform, and it is 20% (w/w) to make water content；
(2) extruding pretreatment：Using the expanded extruder of twin-screw, 100 DEG C of extrusion temperature, nib aperture 20mm, screw rod turn
Fast 110r/min, the soybean protein of extrusion after extruding terminates, is crushed to by soybean protein extrusion process using high speed disintegrator
60 mesh；
(3) the scattered of soybean protein, aquation：By the soyabean protein powder after crushing, quality percentage is formulated as with deionized water
Than the soy bean proteinous soln for 5%, by soy bean proteinous soln is scattered, aquation (shear rate is 2000rpm, time 15min), rise
Temperature adjusts pH value to 8.5 to 50 DEG C with sodium hydroxide solution；
(4) the soybean protein enzyme membrane reaction based on ultra-filtration and separation：Alkali protease is added in the substrate prepared
Alcalase 2.4L (Novi of Denmark letter) 1.5% (add quality to count on the basis of soybean protein raw material quality, similarly hereinafter), constant temperature
50 DEG C, rotating speed 36r/min stirring enzymolysis to system pH be 7.0；Add neutral proteinase Neutrase 0.8L (Novi of Denmark
Letter) 0.75% and flavor protease Flavourzyme 1000L (Novi of Denmark letter) 1.5%, open two-stage ultrafiltering system and carry out
Enzyme membrane reacts, and (molecular cut off is for the trapped fluid of first order milipore filter (molecular cut off is 5000Dal), second level milipore filter
Trapped fluid 1000Dal) is pumped back to retort reuse；When substrate volume reduces 1/4, supplement aliquots of deionized water or steaming
Distilled water；Constant temperature stirring is kept in whole reaction, reaction 6h terminates；
(5) concentrate, sterilize, dry：The permeate of second level milipore filter is concentrated into solid content into evaporation concentrator
30%, concentrate enters back into spray drying tower and dried after being sterilized through ultrahigh-temperature instant sterilization machine, and inlet temperature control is 170 DEG C,
Outlet temperature control is 80 DEG C, obtains composite aminophenol powder 59.7kg.
Embodiment 2
(1) raw material moisture is adjusted：By 100kg soybean protein isolates raw material, (in terms of butt, 92.6%) protein content is
Deionized water is added, dispersed with stirring is uniform, it is 25% (w/w) to make water content；
(2) extruding pretreatment：Using the expanded extruder of twin-screw, 95 DEG C of extrusion temperature, nib aperture 15mm, screw speed
100r/min, by soybean protein extrusion process, after extruding terminates, 40 are crushed to using high speed disintegrator by the soybean protein of extrusion
Mesh；
(3) the scattered of soybean protein, aquation：By the soyabean protein powder after crushing, quality percentage is formulated as with deionized water
Than for 8% soy bean proteinous soln, by soy bean proteinous soln is scattered, aquation (shear rate is 3000rpm, time 10min), heating
To 45 DEG C, pH value is adjusted to 8.0 with sodium hydroxide solution；
(4) the soybean protein enzyme membrane reaction based on ultra-filtration and separation：Alkali protease is added in the substrate prepared
Alcalase 2.4L (Novi of Denmark letter) 1.0% (being counted on the basis of soybean protein raw material quality, similarly hereinafter), 45 DEG C of constant temperature, rotating speed
50r/min stirrings enzymolysis to system pH is 7.5, addition neutral proteinase Neutrase 0.8L (Novi of Denmark letter) 0.5%,
Flavor protease Flavourzyme 1000L (Novi of Denmark letter) 1%, open two-stage ultrafiltering system and carry out enzyme membrane reaction, first
The trapped fluid of level milipore filter (molecular cut off is 5000Dal), the retention of second level milipore filter (molecular cut off is 1000Dal)
Liquid pump returns to retort reuse；When substrate volume reduces 1/4, supplement aliquots of deionized water or distilled water；Whole reaction
Middle holding constant temperature stirring, reaction 4h terminates；
(5) concentrate, sterilize, dry：The permeate of second level milipore filter is concentrated into solid content into evaporation concentrator
25%, concentrate enters back into spray drying tower and dried after being sterilized through ultrahigh-temperature instant sterilization machine, and inlet temperature control is 160 DEG C,
Outlet temperature control is 70 DEG C, obtains composite aminophenol powder 57.2kg.
Embodiment 3
(1) raw material moisture is adjusted：By 100kg soybean protein isolates raw material, (in terms of butt, 92.6%) protein content is
Deionized water is added, dispersed with stirring is uniform, it is 30% (w/w) to make water content；
(2) extruding pretreatment：Using the expanded extruder of twin-screw, 105 DEG C of extrusion temperature, nib aperture 25mm, screw rod turn
Fast 120r/min, the soybean protein of extrusion after extruding terminates, is crushed to by soybean protein extrusion process using high speed disintegrator
80 mesh；
(3) the scattered of soybean protein, aquation：By the soyabean protein powder after crushing, quality percentage is formulated as with deionized water
Than for 10% soy bean proteinous soln, by soy bean proteinous soln is scattered, aquation (shear rate is 2500rpm, time 15min), rise
Temperature adjusts pH value to 8.5 to 55 DEG C with sodium hydroxide solution；
(4) the soybean protein enzyme membrane reaction based on ultra-filtration and separation：Alkali protease is added in the substrate prepared
Alcalase 2.4L (Novi of Denmark letter) 2% (being counted on the basis of soybean protein raw material quality, similarly hereinafter), 55 DEG C of constant temperature, rotating speed
20r/min stirrings enzymolysis to system pH is 7.0, adds neutral proteinase Neutrase 0.8L (Novi of Denmark letter) 1%, wind
Taste Protease F lavourzyme 1000L (Novi of Denmark letter) 2%, open two-stage ultrafiltering system and carry out enzyme membrane reaction, the first order
Trapped fluid, the trapped fluid of second level milipore filter (molecular cut off is 1000Dal) of milipore filter (molecular cut off is 5000Dal)
It is pumped back to retort reuse；When substrate volume reduces 1/4, supplement aliquots of deionized water or distilled water；In whole reaction
Constant temperature stirring is kept, reaction 8h terminates；
(5) concentrate, sterilize, dry：The permeate of second level milipore filter is concentrated into solid content into evaporation concentrator
35%, concentrate enters back into spray drying tower and dried after being sterilized through ultrahigh-temperature instant sterilization machine, and inlet temperature control is 180 DEG C,
Outlet temperature control is 90 DEG C, obtains composite aminophenol powder 62.6kg.
Embodiment 4
(1) raw material moisture is adjusted：By 100kg soybean protein isolates raw material, (in terms of butt, 92.6%) protein content is
Deionized water is added, dispersed with stirring is uniform, it is 25% (w/w mass percents) to make water content；
(2) extruding pretreatment：Using the expanded extruder of twin-screw, 95 DEG C of extrusion temperature, nib aperture 25mm, screw speed
100r/min, by soybean protein extrusion process, after extruding terminates, 60 are crushed to using high speed disintegrator by the soybean protein of extrusion
Mesh；
(3) the scattered of soybean protein, aquation：By the soyabean protein powder after crushing, quality percentage is formulated as with deionized water
Than for 6% soy bean proteinous soln, by soy bean proteinous soln is scattered, aquation (shear rate is 2000rpm, time 20min), heating
To 50 DEG C, pH value is adjusted to 8.0 with sodium hydroxide solution；
(4) the soybean protein enzyme membrane reaction based on ultra-filtration and separation：Alkali protease is added in the substrate prepared
Alcalase 2.4L (Novi of Denmark letter) 1.5% (being counted on the basis of soybean protein raw material quality, similarly hereinafter), 50 DEG C of constant temperature, rotating speed
60r/min stirrings enzymolysis to system pH is 7.5, adds neutral proteinase Neutrase 0.8L (Novi of Denmark letter) 1%, wind
Taste Protease F lavourzyme 1000L (Novi of Denmark letter) 2%, open two-stage ultrafiltering system and carry out enzyme membrane reaction, the first order
Trapped fluid, the trapped fluid of second level milipore filter (molecular cut off is 1000Dal) of milipore filter (molecular cut off is 5000Dal)
It is pumped back to retort reuse；When substrate volume reduces 1/4, supplement aliquots of deionized water or distilled water；In whole reaction
Constant temperature stirring is kept, reaction 6h terminates；
(5) concentrate, sterilize, dry：The permeate of second level milipore filter is concentrated into solid content into evaporation concentrator
30%, concentrate enters back into spray drying tower and dried after being sterilized through ultrahigh-temperature instant sterilization machine, and inlet temperature control is 175 DEG C,
Outlet temperature control is 85 DEG C, obtains composite aminophenol powder 60.5kg.
Embodiment 5
(1) raw material moisture is adjusted：By 100kg soybean protein isolates raw material, (in terms of butt, 92.6%) protein content is
Deionized water is added, dispersed with stirring is uniform, it is 30% (w/w) to make water content；
(2) extruding pretreatment：Using the expanded extruder of twin-screw, 105 DEG C of extrusion temperature, nib aperture 25mm, screw rod turn
Fast 120r/min, the soybean protein of extrusion after extruding terminates, is crushed to by soybean protein extrusion process using high speed disintegrator
80 mesh；
(3) the scattered of soybean protein, aquation：By the soyabean protein powder after crushing, quality percentage is formulated as with deionized water
Than for 10% soy bean proteinous soln, by soy bean proteinous soln is scattered, aquation (shear rate is 2500rpm, time 15min), rise
Temperature adjusts pH value to 8.5 to 55 DEG C with sodium hydroxide solution；
(4) the soybean protein enzyme membrane reaction based on ultra-filtration and separation：Alkali protease (Guangxi is added in the substrate prepared
Pang Bo bioengineering Co., Ltd) 2% (being counted on the basis of soybean protein raw material quality, similarly hereinafter), 55 DEG C of constant temperature, rotating speed 20r/
Min stirrings enzymolysis to system pH is 7.0, adds neutral proteinase (Guangxi Pang Bo bioengineering Co., Ltd) 1%, local flavor egg
White enzyme Flavourzyme 1000L (Novi of Denmark letter) 2%, open two-stage ultrafiltering system and carry out enzyme membrane reaction, first order ultrafiltration
The trapped fluid of film (molecular cut off is 5000Dal), the trapped fluid blowback of second level milipore filter (molecular cut off is 1000Dal)
Reused to retort；When substrate volume reduces 1/4, supplement aliquots of deionized water or distilled water；Kept in whole reaction
Constant temperature is stirred, and reaction 8h terminates；
(5) concentrate, sterilize, dry：The permeate of second level milipore filter is concentrated into solid content into evaporation concentrator
35%, concentrate enters back into spray drying tower and dried after being sterilized through ultrahigh-temperature instant sterilization machine, and inlet temperature control is 180 DEG C,
Outlet temperature control is 90 DEG C, obtains composite aminophenol powder 60.9kg.
Comparative example 1
(1) raw material moisture is adjusted：By 100kg soybean protein isolates raw material, (in terms of butt, 92.6%) protein content is
Deionized water is added, dispersed with stirring is uniform, it is 6% (w/w) to make water content；
(2) it is heat-treated：Soybean protein slurry is warming up to 95 DEG C, 20min is kept, 50 DEG C are cooled to, regulation pH value to
8.5；
(3) the soybean protein enzyme membrane reaction based on ultra-filtration and separation：Alkali protease is added in the substrate prepared
Alcalase 2.4L (Novi of Denmark letter) 2% (being counted on the basis of soybean protein raw material quality, similarly hereinafter), 50 DEG C of constant temperature, rotating speed
20r/min stirrings enzymolysis to system pH is 7.0, adds neutral proteinase Neutrase 0.8L (Novi of Denmark letter) 1%, wind
Taste Protease F lavourzyme 1000L (Novi of Denmark letter) 2%, open two-stage ultrafiltering system and carry out enzyme membrane reaction, the first order
Trapped fluid, the trapped fluid of second level milipore filter (molecular cut off is 1000Dal) of milipore filter (molecular cut off is 5000Dal)
It is pumped back to retort reuse；When substrate volume reduces 1/4, supplement aliquots of deionized water or distilled water；In whole reaction
Constant temperature stirring is kept, reaction 8h terminates；
(4) concentrate, sterilize, dry：The permeate of second level milipore filter is concentrated into solid content into evaporation concentrator
30%, concentrate enters back into spray drying tower and dried after being sterilized through ultrahigh-temperature instant sterilization machine, and inlet temperature control is 180 DEG C,
Outlet temperature control is 90 DEG C, obtains composite aminophenol powder 52.4kg.
Comparative example 2
(1) raw material moisture is adjusted：By 100kg soybean protein isolates raw material, (in terms of butt, 92.6%) protein content is
Deionized water is added, dispersed with stirring is uniform, it is 30% (w/w) to make water content；
(2) extruding pretreatment：Using the expanded extruder of twin-screw, 105 DEG C of extrusion temperature, nib aperture 25mm, screw rod turn
Fast 120r/min, the soybean protein of extrusion after extruding terminates, is crushed to by soybean protein extrusion process using high speed disintegrator
80 mesh；
(3) the scattered of soybean protein, aquation：By the soyabean protein powder after crushing, quality percentage is formulated as with deionized water
Than for 10% soy bean proteinous soln, by soy bean proteinous soln is scattered, aquation (shear rate is 2500rpm, time 15min), rise
Temperature adjusts pH value to 8.5 to 55 DEG C with sodium hydroxide solution；
(4) the soybean protein enzyme membrane reaction based on ultra-filtration and separation：Alkali protease is added in the substrate prepared
Alcalase 2.4L (Novi of Denmark letter) 2% (being counted on the basis of soybean protein raw material quality, similarly hereinafter), neutral proteinase
Neutrase 0.8L (Novi of Denmark letter) 1%, flavor protease Flavourzyme 1000L (Novi of Denmark letter) 2%, are opened
Two-stage ultrafiltering system carries out enzyme membrane reaction, the trapped fluid of first order milipore filter (molecular cut off is 5000Dal), second level ultrafiltration
The trapped fluid of film (molecular cut off is 1000Dal) is pumped back to retort reuse；When substrate volume reduces 1/4, supplement
Aliquots of deionized water or distilled water；(55 DEG C) stirrings (rotating speed 20r/min) of constant temperature are kept in whole reaction, reaction 8h terminates；
(5) concentrate, sterilize, dry：The permeate of second level milipore filter is concentrated into solid content into evaporation concentrator
35%, concentrate enters back into spray drying tower and dried after being sterilized through ultrahigh-temperature instant sterilization machine, and inlet temperature control is 180 DEG C,
Outlet temperature control is 90 DEG C, obtains composite aminophenol powder 55.6kg.
Effect example
Method as defined in GB/T 5009.3, GB/T 5009.4 is respectively adopted and determines embodiment 1~5, the system of comparative example 1~2
Composite aminophenol powder moisture, the content of ashes obtained；Using automatic amino acid analyzer method, fluorescence spectrophotometry [Yang Yuexin, king
Light Asia chief editor eats analyzing food nutrition components of mirowave handbook Beijing:China Light Industry Press, 2002:41-55] analysis embodiment
1~4, composite aminophenol powder free aminoacid content, total amino acid content made from comparative example 1 are (containing cystine, tryptophan etc. 18
Plant amino acid), calculate free amino acid in product and account for total amino acid percentage composition, every group of experiment is parallel three times, averages
For result, 1 the results are shown in Table.
Table 1
From table 1, using embodiment 1~5 (wherein implement 5 dezymotize selection with embodiment 3 different outside without other differences)
There is no notable difference with total amino acid content, moisture, content of ashes in composite aminophenol powder made from comparative example 1~2, but
The product yield of the acquisition of embodiment 1~5, free amino acid account for total amino acid content apparently higher than comparative example 1, it is seen that extrusion process
More traditional heat treating process (95 DEG C, 20min) can significantly increase the hydrolysis degree and free amino acid yield of product.Comparative example
2 from the technique of embodiment 3 dezymotize order of addition it is different it is outer other are identical, but the product yield of comparative example 2 and free amino acid account for total ammonia
Base acid content is below embodiment 3, it is seen that enzyme order of addition influences the yield and quality of product.The product yield of embodiment 1~5 and
It is not notable that free amino acid accounts for total amino acid content difference, and product yield is that 57.2%~62.6%, free amino acid accounts for total ammonia
Base acid content is 44.9%~48.7%, and product free amino acid accounts for total amino acid content more than 40%.
Above-described embodiment is preferably embodiment, but embodiments of the present invention are not by above-described embodiment of the invention
Limitation, other any Spirit Essences without departing from the present invention and the change made under principle, modification, replacement, combine, simplification,
Equivalent substitute mode is should be, is included within protection scope of the present invention.
Claims (7)
1. a kind of method that soybean protein depth growth prepares compound amino acid, it is characterised in that comprise the following steps：
(1) raw material moisture is adjusted：Water is added in soybean protein isolate raw material, is stirred, aqueous soybean protein is obtained；
(2) extruding pretreatment：With the expanded extruder of twin-screw by aqueous soybean protein extrusion process, after extruding terminates, then it will squeeze
The soybean protein gone out is crushed；95~105 DEG C of extrusion temperature, 15~25mm of nib aperture, 100~120r/min of screw speed；
(3) the scattered and aquation of soybean protein：The soyabean protein powder after crushing is formulated as soy bean proteinous soln with water, disperse with
Aquation；Then 45~55 DEG C are warming up to, regulation pH value obtains reaction substrate to 8~9；
(4) the soybean protein enzyme membrane reaction based on ultra-filtration and separation：Basic protein is added in the reaction substrate that step (3) is obtained
Enzyme, it is 7~7.5 to be digested in 45~55 DEG C of constant temperature stirrings to system pHs；Neutral proteinase and flavor protease are subsequently added into, is opened
Open two-stage ultrafiltering system and carry out enzyme membrane reaction, the trapped fluid of first order milipore filter and the trapped fluid of second level milipore filter are pumped back to instead
Tank is answered to reuse；When the volume of reaction substrate reduces 1/4, the water of equal volume amounts is supplemented；Keep permanent in whole course of reaction
Warm stirring；
(5) concentrate, sterilize, dry：The permeate of second level milipore filter is concentrated, obtained concentrate sterilization, spray drying,
Obtain composite aminophenol powder；
The molecular cut off of described first order milipore filter is 5000Dal, and membrane filtration pressure is 0.1~0.5MPa；
The molecular cut off of described second level milipore filter is 1000Dal, and membrane filtration pressure is 0.1~0.5MPa.
2. the method that soybean protein depth growth prepares compound amino acid according to claim 1, it is characterised in that：
The water content in aqueous soybean protein described in step (1) is mass percent 20~30%；
The content of soyabean protein powder is mass percent 5~10% in soy bean proteinous soln described in step (3).
3. the method that soybean protein depth growth prepares compound amino acid according to claim 1, it is characterised in that：Described
The scattered condition with aquation is：Shear rate is 2000~3000rpm, and the time is 10~20min.
4. the method that soybean protein depth growth prepares compound amino acid according to claim 1, it is characterised in that：
The addition of alkali protease described in step (4) is the 1~2% of soybean protein isolate material quality；
The addition of neutral proteinase described in step (4) is the 0.5~1% of soybean protein isolate material quality；
The addition of flavor protease described in step (4) is the 1~2% of soybean protein isolate material quality.
5. the method that soybean protein depth growth prepares compound amino acid according to claim 1, it is characterised in that：
The rotating speed of stirring described in step (4) is 20~80rpm；
The time of enzyme membrane reaction described in step (4) is 4~8h.
6. the method that soybean protein depth growth prepares compound amino acid according to claim 1, it is characterised in that：
The degree of concentration described in step (5) is that solid content reaches weight/mass percentage composition 20~40%；
The mode of described sterilization described in step (5) sterilizes for ultrahigh-temperature instant；
Described spray drying described in step (5) refers to dry by spray drying tower, and operating parameter is that inlet temperature is
160~180 DEG C, outlet temperature is 70~90 DEG C.
7. a kind of compound amino acid, it is characterised in that：Prepared by the method described in any one of claim 1~6, it is swum
Isolated amino acid accounts for total amino acid content more than mass percent 40%.
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| CN106473138A (en) * | 2016-09-26 | 2017-03-08 | 天津保元堂生物科技有限公司 | Donkey-hide gelatin ginseng protein powder and preparation method thereof |
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