CN101591692A - A kind of production method of active small peptide of duck hemocyte - Google Patents
A kind of production method of active small peptide of duck hemocyte Download PDFInfo
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- CN101591692A CN101591692A CNA2009100336539A CN200910033653A CN101591692A CN 101591692 A CN101591692 A CN 101591692A CN A2009100336539 A CNA2009100336539 A CN A2009100336539A CN 200910033653 A CN200910033653 A CN 200910033653A CN 101591692 A CN101591692 A CN 101591692A
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Abstract
The invention discloses a kind of production method of active small peptide of duck hemocyte, adopt enzyme engineering technology, by haemproteins hydrolysis specific enzyme enzymolysis blood cell cytolemma, then the macro-molecular protein that discharges being carried out pacemaker enzyme cuts, make it resolve into the little peptide and the amino acid of small molecular weight, added a certain amount of food flavor enzyme in this haemproteins hydrolysis specific enzyme, can reduce the generation of bitter peptides, improve the palatability of product, this product not only can substitute fish meal and alleviate animal proteinum situation in short supply, the medicated premix that can also partly replace multiple raising immune disease-resistance is really produced the food of natural health.The product of technology gained of the present invention is rich in the bioactive micro peptide of a large amount of molecular weight below 3000 dalton, has the effect of growing of tangible promotion young animal, this preparation technology's advantages of simple, and production cost is low, is suitable for large-scale industrial production.
Description
Technical field
The present invention relates to the biotechnology technical field of enzyme engineering, particularly relate to a kind of production method of active small peptide of duck hemocyte.
Background technology
China's livestock industry is at present constantly risen to the demand of animal proteinum, fish meal is a most important animal protein resource in the fodder industry always, according to not intact statistics, the annual fish meal usage quantity of China is about 1,200,000 tons, and wherein nearly 1,000,000 tons of need are from external import, and foreign exchange spends nearly 400,000,000 dollars, what is more important, world's fish meal output constantly descends at present, and along with the growth requirement of fodder industry constantly increases, the situation of fish meal shortage has become the matter of utmost importance of fodder industry.The usage quantity that how to reduce fish meal in the mixed feed just more and more becomes the research focus of Animal nutrition educational circles.
The current research result discloses, as the key ingredient of aquatic animal with children's livestock and poultry animal perfect compound feed in age, fish meal not merely provides high-quality protein and balance amino acid, the what is more important fish meal protein can resolve into the little peptide that is directly absorbed by small intestine in animal body and have the bioactive peptide of physiological function with some, but the fish meal that generally uses is because the limitation of complete processing at present, little peptide content is limited, shorter owing to fish meal residence time in animal gastrointestinal tract on the other hand, and the secretion of young animal digestive ferment is still insufficient, therefore it is also few that fish meal protein relies on the little peptide quantity of body interior endogenous enzyme degraded generation, and this just requires to add a great deal of fish meal to satisfy the demand of animal to little peptide in this class animal feed formulation.
From the functional angle of little peptide, the active small peptide of duck hemocyte that utilizes the duck hyperglobulinemia to adopt enzyme engineering technology to produce, can not only reduce the consumption of fish meal in the prescription, and, can also overcome the plant protein source of taking usually that utilizes for aquatic animal and substitute the caused food calling problem of fish meal method owing to contain natural food calling composition in the active small peptide of duck hemocyte.
Duck blood accounts for 7～8% of duck total protein concentration, itself is nutritious, gross protein value 18～20%, become " liquid meat ", be a kind of fine animal proteinum raw material, great majority are to sell being spray dried to globulin powder after the blood treatment at present, owing to breaking of blood cell cytolemma in the course of processing is limited, cause the animal specific absorption not high, limited its extensive use at fodder industry.
The present invention adopts enzyme engineering technology, by haemproteins hydrolysis specific enzyme enzymolysis blood cell cytolemma, then the macro-molecular protein that discharges being carried out pacemaker enzyme cuts, make it resolve into the little peptide and the amino acid of small molecular weight, added a certain amount of food flavor enzyme in this haemproteins hydrolysis specific enzyme, can reduce the generation of bitter peptides, improve the palatability of product, this product not only can substitute fish meal and alleviate animal proteinum situation in short supply, the medicated premix that can also partly replace multiple raising immune disease-resistance is really produced the food of natural health.
Summary of the invention
The object of the invention and beneficial effect have provided a kind of production method and technology of utilizing the duck blood cell to produce active small peptide, the active small peptide of duck hemocyte that adopts this production method and explained hereafter to go out not only is rich in the bioactive micro peptide of a large amount of molecular weight below 3000 dalton, also contain protoheme, organic acid, organic iron and vitamin B group, good palatability, can improve the especially immunizing power of young animal of animal, impel it to grow up healthy and sound, reduce the consumption of preventive medicine and curative drug.
Technical solution of the present invention is that its step is as follows:
(1) selects large-scale government official duck factory fresh blood for use, handle through anti-freezing, centrifugation (obtains blood cell after 10000～20000r/min), place enzymolysis equipment, with blood cell: the weight ratio of water=1: 2～6 adds tap water, is warming up to after 40～45 ℃ the sodium hydroxide adjust pH 6.5～8.0 with concentration 0.5mol/L.
(2) enzymolysis.Be warming up to 50～58 ℃, add 0.2～0.6% hyperglobulinemia hydrolysis special protein enzyme, with temperature control device with duck blood cell enzymolysis solution temperature at 55～58 ℃, enzymolysis time 18 hours
(3) enzyme that goes out.Duck blood cell enzymolysis solution is warming up to 80～90 ℃, and constant temperature is kept 20～30min.
(4) centrifugation.The duck blood cell enzymolysis solution that will go out behind the enzyme carries out solid-liquid separation through supercentrifuge, collects supernatant liquor, adds 10% salt acid for adjusting pH value for neutral.
(5) desalting treatment.Adopt strong acid type polystyrol cation exchange resin and strong base anionic polystyrene exchange resin to dress up resin column and carry out desalting treatment.
(6) concentrate.Being concentrated into dry matter content by efficient following current falling film condenser is 10～20%.
(7) drying.By spray-drier concentrated solution is dried to powdery product.
Embodiment
Embodiment 1
(1) selecting large-scale government official duck factory fresh blood for use is raw material, obtain blood cell after the centrifugation (14000r/min), place enzymolysis equipment, then with blood cell: the weight ratio of water=1: 3 adds tap water, is warming up to after 40 ℃ the sodium hydroxide adjust pH 7.0 with concentration 0.5mol/L.
(2) enzymolysis.Be warming up to 56 ℃, press hyperglobulinemia hydrolysis special protein enzyme: the weight ratio of blood cell=1: 300 adds hyperglobulinemia hydrolysis special protein enzyme, with temperature control device with duck blood cell enzymolysis solution temperature at 56 ℃, enzymolysis time 24 hours
(3) enzyme that goes out.Duck blood cell enzymolysis solution is warming up to 90 ℃, and constant temperature is kept 30min.
(4) centrifugation.The duck blood cell enzymolysis solution that will go out behind the enzyme carries out solid-liquid separation through supercentrifuge, collects supernatant liquor, adds 10% salt acid for adjusting pH value for neutral.
(5) desalting treatment.Adopt strong acid type polystyrol cation exchange resin and strong base anionic polystyrene exchange resin to dress up resin column and carry out desalting treatment.
(6) concentrate.Being concentrated into dry matter content by efficient following current falling film condenser is 20%.
(7) drying.By spray-drier concentrated solution is dried to powdery product.
Embodiment 2
(1) selecting large-scale government official duck factory fresh blood for use is raw material, obtain blood cell after the centrifugation (14000r/min), place enzymolysis equipment, then with blood cell: the weight ratio of water=1: 4 adds tap water, is warming up to after 40 ℃ the sodium hydroxide adjust pH 8.0 with concentration 0.5mol/L.
(2) enzymolysis.Be warming up to 58 ℃, press hyperglobulinemia hydrolysis special protein enzyme: the weight ratio of blood cell=1: 200 adds hyperglobulinemia hydrolysis special protein enzyme, with temperature control device with duck blood cell enzymolysis solution temperature at 58 ℃, enzymolysis time 18 hours
(3) enzyme that goes out.Duck blood cell enzymolysis solution is warming up to 90 ℃, and constant temperature is kept 30min.
(4) centrifugation.The duck blood cell enzymolysis solution that will go out behind the enzyme carries out solid-liquid separation through supercentrifuge, collects supernatant liquor, adds 10% salt acid for adjusting pH value for neutral.
(5) desalting treatment.Adopt strong acid type polystyrol cation exchange resin and strong base anionic polystyrene exchange resin to dress up resin column and carry out desalting treatment.
(6) concentrate.Being concentrated into dry matter content by efficient following current falling film condenser is 20%.
(7) drying.By spray-drier concentrated solution is dried to powdery product
Embodiment 3
(1) selecting large-scale government official duck factory fresh blood for use is raw material, obtain blood cell after the centrifugation (14000r/min), place enzymolysis equipment, then with blood cell: the weight ratio of water=1: 5 adds tap water, is warming up to after 40 ℃ the sodium hydroxide adjust pH 7.0 with concentration 0.5mol/L.
(2) enzymolysis.Be warming up to 56 ℃, press hyperglobulinemia hydrolysis special protein enzyme: the weight ratio of blood cell=1: 400 adds hyperglobulinemia hydrolysis special protein enzyme, with temperature control device with duck blood cell enzymolysis solution temperature at 56 ℃, enzymolysis time 36 hours
(3) enzyme that goes out.Duck blood cell enzymolysis solution is warming up to 85 ℃, and constant temperature is kept 40min.
(4) centrifugation.The duck blood cell enzymolysis solution that will go out behind the enzyme carries out solid-liquid separation through supercentrifuge, collects supernatant liquor, adds 5% salt acid for adjusting pH value for neutral.
(5) desalting treatment.Adopt strong acid type polystyrol cation exchange resin and strong base anionic polystyrene exchange resin to dress up resin column and carry out desalting treatment.
(6) concentrate.Being concentrated into dry matter content by efficient following current falling film condenser is 15%.
(7) drying.By spray-drier concentrated solution is dried to powdery product.
Claims (1)
1. the production method of an active small peptide of duck hemocyte is characterized in that production stage is:
(1) selects large-scale government official duck factory fresh blood for use, with obtaining blood cell after 10000～20000r/min centrifugation, place enzymolysis equipment, with blood cell: the weight ratio of water=1: 2～6 adds tap water, is warming up to after 40～45 ℃ the sodium hydroxide adjust pH 6.5～8.0 with concentration 0.5mol/L;
(2) enzymolysis is warming up to 50～58 ℃, adds 0.2～0.6% hyperglobulinemia hydrolysis special protein enzyme, with temperature control device with duck blood cell enzymolysis solution temperature at 55～58 ℃, enzymolysis time 18 hours;
(3) enzyme that goes out is warming up to 80～90 ℃ with duck blood cell enzymolysis solution, and constant temperature is kept 20～30min;
(4) centrifugation, the duck blood cell enzymolysis solution behind the enzyme that will go out carries out solid-liquid separation through supercentrifuge, collects supernatant liquor, adds 10% salt acid for adjusting pH value for neutral;
(5) desalting treatment adopts strong acid type polystyrol cation exchange resin and strong base anionic polystyrene exchange resin to dress up resin column and carries out desalting treatment;
(6) concentrate, being concentrated into dry matter content by efficient following current falling film condenser is 10～20%;
(7) drying is dried to powdery product by spray-drier with concentrated solution.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2009100336539A CN101591692A (en) | 2009-06-25 | 2009-06-25 | A kind of production method of active small peptide of duck hemocyte |
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| CNA2009100336539A CN101591692A (en) | 2009-06-25 | 2009-06-25 | A kind of production method of active small peptide of duck hemocyte |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102342368A (en) * | 2011-09-24 | 2012-02-08 | 赵运生 | Method for producing hemepeptide by using red blood cells of animal blood as raw materials |
| CN103952458A (en) * | 2014-04-30 | 2014-07-30 | 桂林军供生化技术开发有限公司 | Method for preparing active peptides in duck blood through microwave-assisted enzymolysis |
| CN105385734A (en) * | 2015-11-25 | 2016-03-09 | 重庆三零三科技有限公司 | Preparation method of duck blood small molecule peptide |
| CN108056271A (en) * | 2017-12-29 | 2018-05-22 | 浦江县协盈动物饲料技术开发有限公司 | Improve the preparation method of the feed of piglet survival rate |
-
2009
- 2009-06-25 CN CNA2009100336539A patent/CN101591692A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102342368A (en) * | 2011-09-24 | 2012-02-08 | 赵运生 | Method for producing hemepeptide by using red blood cells of animal blood as raw materials |
| CN103952458A (en) * | 2014-04-30 | 2014-07-30 | 桂林军供生化技术开发有限公司 | Method for preparing active peptides in duck blood through microwave-assisted enzymolysis |
| CN105385734A (en) * | 2015-11-25 | 2016-03-09 | 重庆三零三科技有限公司 | Preparation method of duck blood small molecule peptide |
| CN108056271A (en) * | 2017-12-29 | 2018-05-22 | 浦江县协盈动物饲料技术开发有限公司 | Improve the preparation method of the feed of piglet survival rate |
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Application publication date: 20091202 |