CN106942104A - A kind of black sufficient Bao artificial raise seedling method - Google Patents
A kind of black sufficient Bao artificial raise seedling method Download PDFInfo
- Publication number
- CN106942104A CN106942104A CN201710220149.4A CN201710220149A CN106942104A CN 106942104 A CN106942104 A CN 106942104A CN 201710220149 A CN201710220149 A CN 201710220149A CN 106942104 A CN106942104 A CN 106942104A
- Authority
- CN
- China
- Prior art keywords
- bao
- black sufficient
- black
- sufficient
- maturity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Farming Of Fish And Shellfish (AREA)
Abstract
The present invention provides a kind of black sufficient Bao artificial raise seedling method, by adjust periodicity of illumination and light quality mode by maturity of fish gonads for 20 30% black sufficient Bao accelerating to 65 75%.Periodicity of illumination accelerating sexual gland is adjusted again, makes the sperm or maturation of ovum of black sufficient Bao.The black sufficient Bao of gonadal maturation is drying in the shade, H2O2With row's essence or ovulation under NaOH stimulation.Plant Bao and essence or ovulation are arranged in single groove of hastening parturition, sperm can be added according to ovum quantity, improve ovum utilization rate, raising rate of fertilization.Embryonated egg, which is put into after washing ovum in hatchery, hatches.It is transferred to before floating larva after hatching is abnormal by siphonage in the nursery pond for having attachment base, to adhere to, abnormal for children Bao.The method that the present invention is provided is easy to operate, black sufficient Bao gonadal maturation, and row's essence or ovulation rate are higher, and rate of fertilization can reach 85%.Cultivated in addition, the black sufficient Bao of gonad maturity accelerating to 65 75% is positioned in the environment that temperature is 15 DEG C, annual nursery can be easy to.
Description
Technical field
The present invention relates to technical field of aquaculture, more particularly to a kind of black sufficient Bao artificial raise seedling method.
Background technology
Abalone is rare ocean edible shellfish, and the title with " soft gold " is described as first of marine products eight delicacies.Abalone it is fresh
Product edible part contains 24% protein；Contain 40% protein, 33.7% glycogen and other nutrients in dry product
Matter, thus, abalone has higher nutritive value.In addition, also there is abalone improving eyesight qi-restoratives, heat-clearing to grow as rare food materials
The effects such as the moon, blood-nourishing benefit stomach, thus abalone has higher medical value.The nutritive value and medicinal valency having by abalone
Value, thus abalone has market potential value.
China is the country of abalone culture scale and yield maximum in the world, wherein, yield accounts for world's abalone yield
79%.However, the continuous deterioration of marine environment, parent are for many generations cultivated and administrative reason etc. is so that abalone germplasm is constantly degenerated,
And occur disease take place frequently, slow-growing and high death rate phenomenon.To solve the above problems, it is necessary to solve problem of management
New improved seeds are introduced simultaneously, to improve current aquiculture status.
Black sufficient Bao is also known as rainbow Bao, is Zelanian exclusive kind, is also one of abalone maximum in the world.Black sufficient Bao tool
There are strong stress resistance, fast growth and characteristic beautiful in colour etc., thus be a kind of preferable Bao kind of quality.Due to black sufficient Bao
It is similar to northern China marine site in Zelanian growing environment, and the growth temperature of black sufficient Bao in the seawater is about 2-22 DEG C,
Therefore, black sufficient Bao can introduce China's cultivation.At present, the basic fundamental route of abalone fry-rearing is：Gonad maturity accelerating-hasten parturition-by
Smart hatching-attachment-children Bao Peiyu.However, due to the difference and the difference of growing environment of germplasm, using existing abalone fry-rearing
Technology can not promote black sufficient Bao gonad development full maturity, cause the rate of fertilization of black sufficient Bao not high.Not high rate of fertilization is caused
The young of black sufficient Bao is less, and then make it that the quantum of output of black sufficient Bao is less, influences the breeding and Bao inter-species research of black sufficient Bao.Separately
Outside, existing abalone fry-rearing technology is only capable of, in spring and the black sufficient Bao of autumn nursery, causing the nursery of black sufficient Bao the blank phase occur, and then
So that the supply of black sufficient Bao is likely to occur supply shortage phenomenon.
The content of the invention
The present invention provides a kind of black sufficient Bao artificial raise seedling method, is fertilized with solving black sufficient Bao using existing abalone seedling raising method
The problem of rate is not high.
The present invention provides a kind of black sufficient Bao artificial raise seedling method, and this method includes：
S01：The maturity of fish gonads that black sufficient Bao is cultivated under the conditions of water temperature is 14-16 DEG C is developed to 20-30%.
The black sufficient Bao that survives for being introduced into or buying is cultivated naturally under the conditions of ocean temperature is 14-16 DEG C.When cultivating one section
Between after, observe the development degree of black sufficient Bao sexual gland.When the maturity of fish gonads for observing black sufficient Bao reaches 20-30%, it can carry out
Gonad maturity accelerating, in case black sufficient Bao Yumiao.
S02：Make the maturity of fish gonads of the black sufficient Bao by 20-30% acceleratings to 65- by adjusting photoperiod and light quality
75%.
Because black sufficient Bao is the exclusive abalone kind of New Zealand, thus there is black sufficient Bao the illumination for meeting its growth demand to want
Ask.Black sufficient Bao has higher requirement for illumination, and different intensity of illumination, light quality and periodicity of illuminations can make black sufficient Bao Chu
In different physiology phases.Thus, the present invention induces the sexual gland Rapid development of black sufficient Bao by adjusting periodicity of illumination and light quality etc..
When the black sufficient Bao maturity of fish gonads of cultivation reaches 20-30%, the sexual gland of black sufficient Bao is promoted by adjusting periodicity of illumination and light quality
Maturity reaches 65-75%.Black sufficient Bao sexual gland outward appearance is observed, when the maturity of fish gonads of black sufficient Bao reaches 65-75%, stops adjusting
Whole periodicity of illumination.Maturity of fish gonads reaches that 65-75% black sufficient Bao Zuowei kinds Bao is positioned in the environment that temperature is 15 DEG C and cultivated.
It should be noted to change water, cleaning residual bait people, excrement etc. during kind abalone culture, while sea-tangle is periodically fed, to ensure black sufficient Bao
Plant survival and the vitality of Bao.
Because black sufficient Bao grows more slowly in relatively low temperature environment, thus the gonad development of black sufficient Bao is also more slow
Slowly.Therefore, in relatively low temperature environment, the maturity of fish gonads of black sufficient Bao grown naturally by 65-75% reach sexual gland completely into
It is ripe to need the long time.Growing naturally for black sufficient Bao long period can provide sufficient time to nursery, with
Get out nursery place, equipment etc..Because black sufficient Bao breeds for 1 year twice, thus growing naturally for long period can be
The annual nursery of black sufficient Bao provides kind of a Bao, and then whole year can realize black sufficient Bao Yumiao at any time., can such as when cultivating the place free time
So that maturity of fish gonads to be reached to the 65-75% black sufficient further accelerating sexual gland of Bao, the black sufficient Bao gonad maturity accelerating time is saved, is realized black
Row's essence of sufficient Bao or ovulation.
Further, the maturity of fish gonads of black sufficient Bao is induced to reach 70% by adjusting periodicity of illumination and light quality etc..If black
The maturity of fish gonads of sufficient Bao is higher, then the sexual gland of black sufficient Bao is just developed naturally within a short period of time.If during gonadal maturation, field
Ground, equipment etc. can not meet black sufficient Bao Yumiao, then black sufficient Bao meeting nature row essence or ovulation, so that fertilization.But due to environment etc.
The limitation of condition, the smart rate of row or ovulation rate of black sufficient Bao be not high, causes rate of fertilization not high, in turn results in kind of Bao and waste.Thus,
When promoting the gonad development of black sufficient Bao using adjustment periodicity of illumination, maturity of fish gonads can not be too high.If the sexual gland of black sufficient Bao is ripe
Degree is relatively low, then the sexual gland of black sufficient Bao needs the natural development of long period.And relatively low maturity of fish gonads accelerating needs are longer
Time, influence the breeding of black sufficient Bao.In the black sufficient Bao artificial raise seedling method that the present invention is provided, using adjustment periodicity of illumination and light
Matter promotes the maturity of fish gonads of black sufficient Bao to reach 70%.70% maturity of fish gonads can promote kind of a Bao property in black sufficient Bao Yumiao
The quick accelerating of gland, while can also avoid because higher maturity of fish gonads causes black sufficient Bao to develop into naturally within a short period of time
It is ripe.
S03：Water temperature is raised to 17 DEG C, adjustment photoperiod and light quality promote the maturity of fish gonads of the black sufficient Bao to be developed to
95-98%.
After the seedling raise period for determining black sufficient Bao, ocean temperature is increased to 17 DEG C, so that the black suitable sea water temperatures of sufficient Bao Tiqian
Degree, and then be that row's essence or ovulation are prepared.After ocean temperature adjustment, maturity of fish gonads has been reached by adjusting photoperiod and light quality
To 65-75% black sufficient Bao accelerating to 95-98%.The maturity of fish gonads of black sufficient Bao be 95-98% when, sperm and egg development into
It is ripe, embryonated egg can be preferably formed, rate of fertilization is improved.Before this gonad maturity accelerating, the maturity of fish gonads of black sufficient Bao is
65-75% is reached, thus the sexual gland of black sufficient Bao can develop complete in the short period, and the row's of progress essence or ovulation.The present invention is carried
Gonad maturity accelerating method in the black sufficient Bao artificial raise seedling method supplied can adapt to interim nursery place arrangement or be badly in need of the feelings of abalone
Shape, makes up abalone gaps in market.
S04：The single water temperature that is put into is that 17 DEG C hasten parturition is molten after the black sufficient Bao Yingan for being 95-98% by maturity of fish gonads
In liquid, make the black sufficient Bao row essence or ovulation.
Black sufficient Bao needs configured in advance to hasten parturition solution before row's essence or ovulation, in favor of black sufficient Bao row essence or ovulation.Urge
Production solution concrete configuration process be：Purchase commercial grades are analytically pure H2O2(hydrogen peroxide) and NaOH (NaOH).Will
The H of purchase2O2Using distilled water diluting, the H that concentration is 60g/L, volume is 1L is formulated as2O2Solution.NaOH is used into distilled water
It is formulated as the NaOH solution that concentration is 1mol/L, volume is 1L.By the H that concentration is 60g/L2O2, concentration for 1mol/L NaOH with
And the seawater after husky filter is 15 according to volume ratio：11：10000 mixing, mixed solution is solution of hastening parturition.The solution that will hasten parturition is distinguished
It is positioned in multiple grooves of hastening parturition, and the temperature for the solution that controls to hasten parturition is 17 DEG C, in case black sufficient Bao row essence or ovulation.
The stronger kind Bao of gonadal maturation, vitality is selected, in case row's essence or ovulation.Black sufficient Bao kind Bao is in cultivation
During, the impurity such as residual bait, excrement may be infected with body.The impurity such as excrement can be with the essence produced by black sufficient Bao row essence or ovulation
Son or ovum mixing, are contaminated sperm or ovum.To avoid sperm or ovum from being contaminated, the black sufficient Bao needs picked out
Do the wash clean.For the black sufficient Bao row essence of stimulation or ovulation, the black sufficient Bao after doing the wash totally, which will dry in the shade, to be handled 1 hour.To improve black foot
The rate of fertilization of Bao, needs to collect the sperm and ovum of black sufficient Bao respectively in the present invention, thus the black sufficient Bao after drying in the shade is single puts
It is placed in groove of hastening parturition.The H hastened parturition in solution2O2Ozone can be produced with NaOH reactions, the ozone of generation can stimulate black sufficient Bao production
Raw more trypsase.Trypsase can promote the secretion of black sufficient Bao promoting sexual gland hormone, so promote black sufficient Bao row essence or
Ovulation, and black sufficient Bao has larger row's essence or ovulation amount.Black sufficient Bao is positioned over groove of hastening parturition and just starts row's essence or row after 4-5 hours
Ovum, and the smart rate of row and ovulation rate can reach 50%.
S05：The sperm and ovum of the black sufficient Bao are taken out, embryonated egg is formed after mixing.
Take out the sperm and ovum hastened parturition in groove, sperm and ovum are still located at hastening parturition in solution.It will be located in solution of hastening parturition
Sperm and ovum mixing, sperm and ovum complete fertilization process, form embryonated egg.Because kind of Bao is respectively in single groove of hastening parturition
Known to sperm and ovum amount in row's essence or ovulation, single groove of hastening parturition, it is thus possible to which the ovum quantity in groove of hastening parturition adds
Plus appropriate sperm so that each ovum is fertilized, and improves rate of fertilization.In the artificial raise seedling method that the present invention is provided, black sufficient Bao
Rate of fertilization can reach 85%.
S06：The embryonated egg, which is washed after ovum, to be transferred in the hatchery that intensity of illumination is 200lx, and hatching forms floating larva.
By the developmental state of micro- sem observation embryonated egg, in order to by wash ovum wash away the upper excessive sperm of embryonated egg with
And other secretion in black sufficient Bao row essence or ovulation process.To prevent secretion and excessive sperm pollutes embryonated egg, when logical
Cross microscope find embryonated egg be in two differentiation period when, just embryonated egg is taken from solution of hastening parturition, so as to carry out washing ovum process.
Wash the embryonated egg after ovum and be directly placed into ocean temperature to carry out hatching process in 17 DEG C of hatchery, swum with being formed
The young.Because the rate of fertilization of black sufficient Bao is not hatched also, to increase the incubation rate of black sufficient abalone fertilized ova, the intensity of illumination of hatchery compared with
It is low, about 200lx.In the present invention, the incubation rate of black sufficient abalone fertilized ova is about 70-85%.
S07：The floating larva is transferred in the nursery pond for having attachment base, the floating larva metamorphosis is young Bao.
Black sufficient Bao prepares the attachment base of floating larva arranging smart or preovulatory needs in advance.In the present invention, floating larva
Attachment base for inoculation benthic diatom corrugated plating.Corrugated plating inoculation benthic diatom specific preparation process be：On corrugated plating
Benthic diatom is inoculated with, benthic diatom is after the culture of about 15 days, and the color of benthic diatom gradually becomes deep brown on corrugated plating
During color, second of expansion culture can be carried out.Third time can be carried out according to black sufficient Bao Yumiao scale demands, expand training the 4th time
Support, until meeting nursery demand.
The embryonated egg of black sufficient Bao is after 65-75h is formed, and most floating larva reaches face later stage, the bottom of into
Dwell the stage of crawling.Into bottom dwell the stage of crawling floating larva it is abnormal for young Bao in the post-attachment, thus need by floating larva from
It is transferred in hatchery in the nursery pond with attachment base.Floating larva is transferred to before nursery pond, it is necessary to thoroughly clear to nursery pond
Reason and sterilization, to reduce germ and Copepods.17 are set to make floating larva adapt to ocean temperature in nursery pond, nursery pond
℃.Because floating larva is that, without vertebra state, may injure floating larva by the form fished for, cause unnecessary damage
Lose.To avoid floating larva from coming to harm, in the present invention, floating larva is shifted by siphonage.Floating larva is from incubating
Change to be during pond is transferred to nursery pond and operated under dark condition, reduce light stimulation, and then improve floating larva
Survival rate.The input of floating larva is close in the maximized utilization nursery pond in the case where ensureing the space of floating larva growth, nursery pond
Spend for 1 × 105/m2。
Floating larva input nursery pond needs shading treatment in 2-3 days, to reduce stimulation of the illumination to floating larva.Swim
Larval metamorphosis starts through-flow water, bait throwing in be attached to after young Bao in attachment base after young Bao attachment, so that young Bao is in water circulation state
Lower growth.According to adhesion condition increased water flow amount of the young Bao in attachment base, and air is continuously passed through, to provide sufficient to young Bao
Oxygen.Young Bao will apply fertilizer in good time during cultivation, to promote the growth of benthic diatom.In addition, Copepods usually attacks
Young Bao, causes young Bao dead, and then reduce black sufficient Bao Yumiao quantum of output.For reduction nursery pond in Copepods quantity, it is necessary to
Copepods is irregularly removed with metrifonate.
The technical scheme that embodiments of the invention are provided can include the following benefits：
In the present invention provides a kind of black sufficient Bao artificial raise seedling method, black sufficient Bao Ziran is cultivated to maturity of fish gonads it is
20-30%.The maturity of fish gonads accelerating of black sufficient Bao is realized black to 65-75% by way of adjusting periodicity of illumination and light quality
The first time gonad maturity accelerating of sufficient Bao.The black sufficient Bao of first time gonad maturity accelerating is positioned in the environment that temperature is 15 DEG C and cultivated.By
More can slowly it be grown in relatively low temperature environment in black sufficient Bao, thus the sexual gland of black sufficient Bao also can slowly grow hair
Educate, be only that maturity is grown and reaches that the sexual gland full ripe time is longer naturally by 65-75% until full maturity.It is black
Growing naturally for sufficient Bao long period can provide the sufficient time to nursery.Because black sufficient Bao breeds for 1 year twice, because
And growing naturally for long period enables to the black sufficient Bao Quannian to have kind of a Bao, and then whole year can realize black sufficient Bao at any time
Nursery.When needing nursery, maturity of fish gonads has further been reached to 65-75% black foot by adjusting periodicity of illumination and light quality
Bao accelerating is to 95-98% so that the sperm or egg development of black sufficient Bao are ripe, beneficial to fertilization, and then improves rate of fertilization.Sexual gland is sent out
Be bred as ripe black sufficient Bao drying in the shade, H2O2With under NaOH stimulation arrange essence or ovulation, and row essence or ovulation amount it is more, can reach
50%.Because kind of Bao arranges essence or ovulation in single groove of hastening parturition respectively, the sperm and ovum amount in single groove of hastening parturition, it is known that because
And ovum quantity that can be in groove of hastening parturition adds appropriate sperm so that each ovum is fertilized, and improves rate of fertilization.Fertilization
It is floating larva that ovum is put into hatching in the hatchery that water temperature is 17 DEG C after washing ovum.Floating larva reaches logical during face later stage
Cross siphonage to be transferred in the nursery pond for having attachment base, so as to abnormal for young Bao after adhering to.The black sufficient Bao people that the present invention is provided
Work method for culturing seedlings promotes black sufficient Bao property gland maturity by adjusting photoperiod and light quality, and by drying in the shade, H2O2Stimulation with NaOH is arranged
Essence or ovulation, and then form embryonated egg.The method that the present invention is provided is easy to operate, black sufficient Bao gonadal maturation, row's essence or row
Ovum rate is higher, and rate of fertilization can reach 85%.
It should be appreciated that the general description of the above and detailed description hereinafter are only exemplary and explanatory, not
Can the limitation present invention.
Brief description of the drawings
Accompanying drawing herein is merged in specification and constitutes the part of this specification, shows the implementation for meeting the present invention
Example, and for explaining principle of the invention together with specification.
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
The accompanying drawing to be used needed for having technology description is briefly described, it should be apparent that, for those of ordinary skill in the art
Speech, without having to pay creative labor, can also obtain other accompanying drawings according to these accompanying drawings.
Fig. 1 is the schematic flow sheet of black sufficient Bao artificial raise seedling method provided in an embodiment of the present invention.
Embodiment
Here exemplary embodiment will be illustrated in detail, its example is illustrated in the accompanying drawings.Following description is related to
During accompanying drawing, unless otherwise indicated, the same numbers in different accompanying drawings represent same or analogous key element.Following exemplary embodiment
Described in embodiment do not represent and the consistent all embodiments of the present invention.On the contrary, they be only with it is such as appended
The example of the consistent apparatus and method of some aspects be described in detail in claims, the present invention.
Accompanying drawing 1 is refer to, accompanying drawing 1 shows the flow signal of black sufficient Bao artificial raise seedling method provided in an embodiment of the present invention
Figure.The description of specific examples below is based on accompanying drawing 1.
Embodiment 1
The present invention provides a kind of black sufficient Bao artificial raise seedling method, and this method includes：
S101：The black sufficient Bao for being introduced into or buying is cultivated under the conditions of ocean temperature is 14 DEG C, until the sexual gland of black sufficient Bao
Maturity is developed to 20-30%.
S102：Make the maturity of fish gonads of black sufficient Bao by 20% accelerating to 65% by adjusting photoperiod and light quality.
S103：After the maturity of fish gonads of black sufficient Bao reaches 65%, rise water temperature is to 17 DEG C, while adjusting photoperiod and light quality
The maturity of fish gonads of black sufficient Bao is promoted to be developed to 95% by 65%.
S104：By the black sufficient Bao Yingan 1h that maturity of fish gonads is 95%, the single water temperature that is put into of black sufficient Bao after drying in the shade is 17
DEG C solution of hastening parturition in so that black sufficient Bao row essence or ovulation.
S105：The sperm and ovum in solution of hastening parturition are taken out respectively, sperm and ovum are mixed, fertilization forms embryonated egg.
S106：Embryonated egg, which is washed after ovum, to be transferred in the hatchery that intensity of illumination is 200lx, and incubating oosperm forms the children that swims
Body.
S107：Floating larva grown and dwelt the bottom of into after the stage of crawling, and is transferred to floating larva by siphonage and has
In the nursery pond of attachment base, floating larva metamorphosis is young Bao.
In black sufficient Bao artificial raise seedling method provided in an embodiment of the present invention, the smart rate of row of black sufficient Bao is 45%, and ovulation rate is
43%, rate of fertilization is 80%, and incubation rate is 75%.
Embodiment 2
The present invention provides a kind of black sufficient Bao artificial raise seedling method, and this method includes：
S201：The black sufficient Bao for being introduced into or buying is cultivated under the conditions of ocean temperature is 16 DEG C, until the sexual gland of black sufficient Bao
Maturity is developed to 30%.
S202：Make the maturity of fish gonads of black sufficient Bao by 30% accelerating to 75% by adjusting photoperiod and light quality.Sexual gland into
The black sufficient Bao of ripe degree accelerating to 75% cultivates under the conditions of temperature is 15 DEG C, to provide kind of a Bao to black sufficient Bao Quannian nursery.
S203：After the maturity of fish gonads of black sufficient Bao reaches 75%, rise water temperature is to 17 DEG C, while adjusting photoperiod and light quality
The maturity of fish gonads of black sufficient Bao is promoted to be developed to 98% by 75%.
S204：The H that concentration is 60g/L is respectively configured2O2Solution and the NaOH solution that concentration is 1mol/L, be by concentration
60g/L H2O2Seawater after NaOH solution that solution, concentration are 1mol/L and husky filter is 15 according to volume ratio：11：10000 mix
Formation is closed to hasten parturition solution.By the black sufficient Bao Yingan 1h that maturity of fish gonads is 98%, the single water temperature that is put into of black sufficient Bao after drying in the shade is
In 17 DEG C of solution of hastening parturition, so that black sufficient Bao row essence or ovulation.
S205：The sperm and ovum in solution of hastening parturition are taken out respectively, sperm and ovum are mixed, fertilization forms embryonated egg.
S206：Pass through the developmental state of micro- sem observation embryonated egg.It is right when observing that embryonated egg breaks up period in two
Embryonated egg washes ovum.Embryonated egg, which is washed after ovum, to be transferred in the hatchery that intensity of illumination is 200lx, the children so that incubating oosperm formation is swum
Body.
S207：Floating larva grown and dwelt the bottom of into after the stage of crawling, will be floating under non-illuminated conditions by siphonage
The young is transferred in the nursery pond for having attachment base for trip, and floating larva metamorphosis is young Bao.Wherein, in nursery pond floating larva input
Density is 1 × 105/m2；Attachment base is the benthic diatom that corrugated plating is inoculated with.After young Bao adheres in attachment base, start to ventilate, lead to
Flowing water.In addition, gradually increasing water-carrying capacity according to the adhesion condition of young Bao.Young Bao will apply fertilizer in good time during cultivation, to promote
Enter the growth of benthic diatom.Meanwhile, the Copepods in nursery pond is irregularly removed using metrifonate.
In black sufficient Bao artificial raise seedling method provided in an embodiment of the present invention, the smart rate of row of black sufficient Bao is 48%, and ovulation rate is
49%, rate of fertilization is 85%, and incubation rate is 80%.
Embodiment 3
The present invention provides a kind of black sufficient Bao artificial raise seedling method, and this method includes：
S301：The black sufficient Bao for being introduced into or buying is cultivated under the conditions of ocean temperature is 15 DEG C, until the sexual gland of black sufficient Bao
Maturity is developed to 25%.
S302：Make the maturity of fish gonads of black sufficient Bao by 25% accelerating to 70% by adjusting photoperiod and light quality.Sexual gland into
The black sufficient Bao of ripe degree accelerating to 70% cultivates under the conditions of temperature is 15 DEG C, to provide kind of a Bao to black sufficient Bao Quannian nursery.
S303：After the maturity of fish gonads of black sufficient Bao reaches 70%, rise water temperature is to 17 DEG C, while adjusting photoperiod and light quality
The maturity of fish gonads of black sufficient Bao is promoted to be developed to 98% by 70%.
S304：The H that concentration is 60g/L is respectively configured2O2Solution and the NaOH solution that concentration is 1mol/L, be by concentration
60g/L H2O2Seawater after NaOH solution that solution, concentration are 1mol/L and husky filter is 15 according to volume ratio：11：10000 mix
Formation is closed to hasten parturition solution.By the black sufficient Bao Yingan 1h that maturity of fish gonads is 98%, the single water temperature that is put into of black sufficient Bao after drying in the shade is
In 17 DEG C of solution of hastening parturition, so that black sufficient Bao row essence or ovulation.
S305：The sperm and ovum in solution of hastening parturition are taken out respectively, sperm and ovum are mixed, fertilization forms embryonated egg.
S306：Pass through the developmental state of micro- sem observation embryonated egg.It is right when observing that embryonated egg breaks up period in two
Embryonated egg washes ovum.Embryonated egg, which is washed after ovum, to be transferred in the hatchery that intensity of illumination is 200lx, the children so that incubating oosperm formation is swum
Body.
S307：Floating larva grown and dwelt the bottom of into after the stage of crawling, will be floating under non-illuminated conditions by siphonage
The young is transferred in the nursery pond for having attachment base for trip, and floating larva metamorphosis is young Bao.Wherein, in nursery pond floating larva input
Density is 1 × 105/m2；Attachment base is the benthic diatom that corrugated plating is inoculated with.After young Bao adheres in attachment base, start to ventilate, lead to
Flowing water.In addition, gradually increasing water-carrying capacity according to the adhesion condition of young Bao.Young Bao will apply fertilizer in good time during cultivation, to promote
Enter the growth of benthic diatom.Meanwhile, the Copepods in nursery pond is irregularly removed using metrifonate.
In black sufficient Bao artificial raise seedling method provided in an embodiment of the present invention, the smart rate of row of black sufficient Bao is 50%, and ovulation rate is
50%, rate of fertilization is 85%, and incubation rate is 85%.
Those skilled in the art will readily occur to its of the present invention after the disclosure that specification and practice are invented here is considered
Its embodiment.The application be intended to the present invention any modification, purposes or adaptations, these modifications, purposes or
Person's adaptations follow the general principle of the present invention and including undocumented common knowledge in the art of the invention
Or conventional techniques.Description and embodiments are considered only as exemplary, and true scope and spirit of the invention are by following
Claim is pointed out.
It should be appreciated that the relational terms of such as " first " and " second " or the like be used merely to an entity or
Operation makes a distinction with another entity or operation, and not necessarily requires or imply these entities or exist between operating any
This actual relation or order.The invention is not limited in the precision architecture for being described above and being shown in the drawings,
And various modifications and changes can be being carried out without departing from the scope.The scope of the present invention is only limited by appended claim
System.
Claims (10)
1. a kind of black sufficient Bao artificial raise seedling method, it is characterised in that methods described includes：
The maturity of fish gonads that black sufficient Bao is cultivated under the conditions of water temperature is 14-16 DEG C is developed to 20-30%；
Make the maturity of fish gonads of the black sufficient Bao by 20-30% acceleratings to 65-75% by adjusting photoperiod and light quality；
Water temperature is raised to 17 DEG C, adjustment photoperiod and light quality promote the maturity of fish gonads of the black sufficient Bao to be developed to 95-98%；
It is single after the black sufficient Bao Yingan for being 95-98% by maturity of fish gonads to be put into the solution of hastening parturition that water temperature is 17 DEG C, make
The black sufficient Bao row essence or ovulation；
The sperm and ovum of the black sufficient Bao are taken out, embryonated egg is formed after mixing；
The embryonated egg, which is washed after ovum, to be transferred in the hatchery that intensity of illumination is 200lx, and hatching forms floating larva；
The floating larva is transferred in the nursery pond for having attachment base, the floating larva metamorphosis is young Bao.
2. black sufficient Bao artificial raise seedling method according to claim 1, it is characterised in that the solution of hastening parturition is that concentration is
60g/L H2O2, concentration be 1mol/L NaOH and seawater according to volume ratio be 15：11：10000 mixed liquors formed.
3. black sufficient Bao artificial raise seedling method according to claim 1, it is characterised in that by siphonage by the children that swims
Body is transferred to the nursery pond for having attachment base.
4. black sufficient Bao artificial raise seedling method according to claim 1, it is characterised in that maturity of fish gonads accelerating to 65-
The 75% black sufficient Bao cultivates under the conditions of temperature is 15 DEG C, annual standby.
5. black sufficient Bao artificial raise seedling method according to claim 4, it is characterised in that made by adjusting photoperiod and light quality
The maturity of fish gonads accelerating of the black sufficient Bao is to 70%.
6. black sufficient Bao artificial raise seedling method according to claim 1, it is characterised in that it is institute that the embryonated egg, which washes ovum period,
State the differentiation period of embryonated egg two.
7. black sufficient Bao artificial raise seedling method according to claim 1, it is characterised in that the floating larva is transferred to described educate
No light during Miao Chi.
8. black sufficient Bao artificial raise seedling method according to claim 1, it is characterised in that swim children described in the nursery pond
The input density of body is 1 × 105/m2。
9. black sufficient Bao artificial raise seedling method according to claim 1, it is characterised in that the attachment base is inoculated with for corrugated plating
Benthic diatom.
10. black sufficient Bao artificial raise seedling method according to claim 1, it is characterised in that methods described also includes：
Ventilation, through-flow water after the young Bao attachment, and gradually increase water-carrying capacity；Fertilising, dispenser in good time.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710220149.4A CN106942104A (en) | 2017-04-06 | 2017-04-06 | A kind of black sufficient Bao artificial raise seedling method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710220149.4A CN106942104A (en) | 2017-04-06 | 2017-04-06 | A kind of black sufficient Bao artificial raise seedling method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106942104A true CN106942104A (en) | 2017-07-14 |
Family
ID=59474431
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710220149.4A Pending CN106942104A (en) | 2017-04-06 | 2017-04-06 | A kind of black sufficient Bao artificial raise seedling method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106942104A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107549068A (en) * | 2017-08-25 | 2018-01-09 | 江苏省海洋水产研究所 | A kind of device and method for improving clam incubation rate |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101167444A (en) * | 2007-11-28 | 2008-04-30 | 厦门大学 | Haliotis sieboldii and Haliotis discus hannai interspecific hybridization seed production method |
| CN102017913A (en) * | 2010-08-31 | 2011-04-20 | 华南理工大学 | Energy-saving method for culturing haliotis diversicolor aquatilis |
| CN105475195A (en) * | 2015-12-14 | 2016-04-13 | 临沂大学 | Method for harmlessly cultivating haliotis diversicolor supertexta juvenile mollusk through waste gasoline drum |
| CN105494187A (en) * | 2015-12-08 | 2016-04-20 | 山东大学（威海） | Haliotis discus hannnai and haliotis iris interspecific hybrid production method |
| CN106376494A (en) * | 2015-08-05 | 2017-02-08 | 胡庆昆 | Abalone indoor artificial culture method |
-
2017
- 2017-04-06 CN CN201710220149.4A patent/CN106942104A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101167444A (en) * | 2007-11-28 | 2008-04-30 | 厦门大学 | Haliotis sieboldii and Haliotis discus hannai interspecific hybridization seed production method |
| CN102017913A (en) * | 2010-08-31 | 2011-04-20 | 华南理工大学 | Energy-saving method for culturing haliotis diversicolor aquatilis |
| CN106376494A (en) * | 2015-08-05 | 2017-02-08 | 胡庆昆 | Abalone indoor artificial culture method |
| CN105494187A (en) * | 2015-12-08 | 2016-04-20 | 山东大学（威海） | Haliotis discus hannnai and haliotis iris interspecific hybrid production method |
| CN105475195A (en) * | 2015-12-14 | 2016-04-13 | 临沂大学 | Method for harmlessly cultivating haliotis diversicolor supertexta juvenile mollusk through waste gasoline drum |
Non-Patent Citations (3)
| Title |
|---|
| 张恩溢: "新西兰的彩虹鲍育苗新技术", 《福建水产》 * |
| 郭战胜: "皱纹盘鲍与黑足鲍杂交育苗研究", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
| 郭战胜等: "皱纹盘鲍(Haliotis discus hannai)与黑足鲍(Haliotis iris)杂交育苗的初步研究", 《山东大学学报(理学版)》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107549068A (en) * | 2017-08-25 | 2018-01-09 | 江苏省海洋水产研究所 | A kind of device and method for improving clam incubation rate |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104160993B (en) | The artificial breeding method of a kind of Paracobitis variegates fry | |
| CN102232362A (en) | Biological cultivation method of sea horse | |
| CN104161000B (en) | A kind of cross breeding method of hybridizing carp and Pengzhe fish | |
| CN101743934A (en) | Non-pollution culture method of Bohai Bay perinereis aibuhiensis grubes | |
| CN101317550A (en) | Artificial nursery stock growing method for hybridized mussel | |
| CN105918168B (en) | Exopalaemon carinicauda saline-alkali water artificial cultivating method | |
| CN104957065A (en) | Ecological breeding method of breeding penaeus vannamei boone, crucian and chub in mixed mode | |
| CN109247275A (en) | A kind of method for pearl culture | |
| LU502637B1 (en) | Method for promoting maturation of coelomactra antiquata parent shellfish cultured by shrimp pond algae | |
| CN107581106A (en) | A kind of artificial fecundation method of platax orbicularis | |
| CN1181733C (en) | Artificially cultured triploid Hepu nacre and its pearl culturing method | |
| KR101768577B1 (en) | Method of culturing of cold water species using rotifer as live food | |
| CN1172581C (en) | Artificial reproduction method of large salamander | |
| CN101622974B (en) | Soilless Nereid larva breeding method | |
| CN109892261B (en) | Artificial breeding method of Babuyan jellyfishes | |
| CN107751050B (en) | Batched rhinogobio ventralis fry breeding method | |
| CN108323462A (en) | A kind of breeding method of resistance to less salt chief blood clam seed | |
| CN1401225A (en) | Method for overwintering and intermidiate breeding clam fry | |
| CN104823889A (en) | Breeding method for scallop hybridization breeding | |
| CN102090357B (en) | Factory culturing method for clam shells | |
| CN110050735A (en) | A kind of dwarf clam introduces a fine variety mating system | |
| CN101933470A (en) | Parent cultivation method for batch production of ricefield eel offspring seeds | |
| CN104145868B (en) | A kind of method improving pond loach fry survival rate | |
| CN100512639C (en) | Plecoglossus altivelis artificial reproduction method | |
| CN106942104A (en) | A kind of black sufficient Bao artificial raise seedling method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170714 |
|
| RJ01 | Rejection of invention patent application after publication |