CN106900604B - Artificial induced spawning method for wild hucho taimen - Google Patents
Artificial induced spawning method for wild hucho taimen Download PDFInfo
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- CN106900604B CN106900604B CN201710100206.5A CN201710100206A CN106900604B CN 106900604 B CN106900604 B CN 106900604B CN 201710100206 A CN201710100206 A CN 201710100206A CN 106900604 B CN106900604 B CN 106900604B
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- 241001154287 Hucho taimen Species 0.000 title claims abstract description 34
- 238000000034 method Methods 0.000 title claims abstract description 10
- 241000251468 Actinopterygii Species 0.000 claims abstract description 56
- 235000019688 fish Nutrition 0.000 claims abstract description 56
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 26
- 230000002196 ecbolic effect Effects 0.000 claims abstract description 11
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 8
- 239000008103 glucose Substances 0.000 claims abstract description 8
- 239000008239 natural water Substances 0.000 claims abstract description 6
- 230000000638 stimulation Effects 0.000 claims abstract description 6
- 238000012258 culturing Methods 0.000 claims abstract description 5
- 210000000582 semen Anatomy 0.000 claims abstract description 3
- 239000008194 pharmaceutical composition Substances 0.000 claims description 12
- 241000972773 Aulopiformes Species 0.000 claims description 6
- 235000019515 salmon Nutrition 0.000 claims description 6
- 102000011022 Chorionic Gonadotropin Human genes 0.000 claims description 5
- 108010062540 Chorionic Gonadotropin Proteins 0.000 claims description 5
- 229940015047 chorionic gonadotropin Drugs 0.000 claims description 5
- 238000005303 weighing Methods 0.000 claims description 5
- VZCYOOQTPOCHFL-UPHRSURJSA-N maleic acid Chemical compound OC(=O)\C=C/C(O)=O VZCYOOQTPOCHFL-UPHRSURJSA-N 0.000 claims description 4
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 claims description 4
- 235000013601 eggs Nutrition 0.000 abstract description 11
- 241001465754 Metazoa Species 0.000 abstract description 6
- 230000001939 inductive effect Effects 0.000 abstract description 2
- 102000002322 Egg Proteins Human genes 0.000 abstract 1
- 108010000912 Egg Proteins Proteins 0.000 abstract 1
- 210000004681 ovum Anatomy 0.000 abstract 1
- 230000032696 parturition Effects 0.000 abstract 1
- 230000004936 stimulating effect Effects 0.000 abstract 1
- 239000003085 diluting agent Substances 0.000 description 5
- 239000000243 solution Substances 0.000 description 4
- 241000276450 Hucho Species 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- 230000001681 protective effect Effects 0.000 description 3
- 241000894007 species Species 0.000 description 3
- 238000011144 upstream manufacturing Methods 0.000 description 3
- 241000277331 Salmonidae Species 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000004720 fertilization Effects 0.000 description 2
- 230000006698 induction Effects 0.000 description 2
- 241000876433 Acipenser dabryanus Species 0.000 description 1
- 241000883303 Acipenser sinensis Species 0.000 description 1
- 241000252349 Acipenser transmontanus Species 0.000 description 1
- 241001117159 Hucho bleekeri Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
- A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
- A61K31/445—Non condensed piperidines, e.g. piperocaine
- A61K31/4523—Non condensed piperidines, e.g. piperocaine containing further heterocyclic ring systems
- A61K31/454—Non condensed piperidines, e.g. piperocaine containing further heterocyclic ring systems containing a five-membered ring with nitrogen as a ring hetero atom, e.g. pimozide, domperidone
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/60—Fish, e.g. seahorses; Fish eggs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/22—Hormones
- A61K38/24—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g. HCG; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Chemical & Material Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Pharmacology & Pharmacy (AREA)
- Marine Sciences & Fisheries (AREA)
- Endocrinology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Engineering & Computer Science (AREA)
- Gastroenterology & Hepatology (AREA)
- Reproductive Health (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention relates to an artificial spawning method for wild hucho taimen, which is characterized in that male fishes and female fishes growing naturally are selected when the water temperature of a growing water area of the wild hucho taimen is 6.2-7.5 ℃ every year, and the tails are counted and weighed respectively; injecting 0.4mL/kg of oxytocic composition diluted with glucose at 1/3 below chest fin base of female parent fish, and culturing female and male parent fish in the pond at a ratio of 2-3:1, and performing uninterrupted running water stimulation; stimulating with running water for 10-11 hr, collecting ovum and semen from male and female, and inducing parturition, and placing male fish and female fish into natural water area. According to the scheme of the invention, fertilized eggs are obtained, and used wild hucho taimen parent fish can be reasonably and effectively protected, so that the technical problem that the fertilized eggs are difficult to obtain by domesticating the wild hucho taimen at the present stage can be solved, the protection of national class I protected animals can be promoted, and the ecological benefit is remarkable.
Description
Technical Field
The invention relates to the technical field of cultivation, in particular to an artificial induced spawning method for wild hucho taimen.
Background
Huchobleekeri huchoiseikerri belongs to Salmoniformes Salmo niformes Salmonidae Hucho, and is a national class I protective animal. Belongs to the specific large-scale salmonidae fishes at the upstream of Yangtze river, is also the only large-scale native salmonidae fishes in the Qinghai-Tibet plateau area, is a national class I protective animal, and in the Chinese biodiversity protection plan provided in 11 months 1992, the hucho taimen in Chuanshan mountain is listed in a class I fish priority protective species list, which is inferior to white sturgeons, Chinese sturgeons and acipenser dabryanus, and listed in the fourth place; the hucho taimen, Chuanshan hucho salmon, published in 1998 as the endangered species and in red book of endangered animals, 2004 as the red famous records of Chinese species. The hucho taimen is the soutest distributed type of 5 fishes in hucho genus, is diffused from the north when the climate is cold in the period of the fourth glacier, is left in rivers with higher altitude and lower water temperature after the ice period is over, is a typical land-sealed type fish, and has important academic value in the aspects of researching animal geography, ancient ecology, fish system evolution, climate change and the like. Hucho taimen are historically distributed in Sichuan (the great river and Minjiang river upstream and tributaries), Qinghai (the Makohe) and Shaanxi (the Hanjiang upstream tributaries are the white river and the xu water river), and the like. Since the 60's of the 20 th century, the population number has decreased dramatically due to climate change and human activities, and it is now difficult to collect samples in multiple historical distribution areas.
The age of the primary mature of hucho taimen is 4-6 years, wild hucho taimen captured from the field is difficult to domesticate and has extremely high mortality rate, and female and male parent fishes which are mature while domesticating are more difficult to add. The wild hucho taimen domesticated to obtain parent fish capable of being bred is technically extremely difficult. The hucho taimen is cultured, so that after mature female and male parent fishes of hucho taimen are obtained from the field, artificial induced spawning is immediately carried out, fertilized eggs are obtained, hucho taimen larvae are obtained through artificial fertilization, incubation and seedling cultivation, and the hucho taimen larvae are cultured into parent fishes capable of being propagated, so that the hucho taimen is an important means and measure for protecting hucho taimen resources.
Disclosure of Invention
The invention aims to overcome the defect that fertilized eggs of hucho taimen are difficult to obtain under domesticated conditions, and provides an induced spawning method for obtaining fertilized eggs by catching wild hucho taimen parent fishes and inducing spawns and sperms to synchronously develop under artificial control under field conditions so as to induce the sperms and the ovas to mature and discharge under field conditions to obtain the fertilized eggs.
In order to achieve the purpose, the invention adopts the technical scheme that: an artificial induced spawning method for wild hucho taimen, comprising the following steps:
(1) when the water temperature of a growing water area of wild hucho taimen every year is 6.2-7.5 ℃, selecting male fishes with the weight of more than 4kg and female fishes with the weight of more than 6.5kg under natural growth conditions, respectively counting the tails and weighing;
(2) injecting 0.4mL/kg of oxytocic pharmaceutical composition diluted by glucose with mass concentration of 5% into 1/3 below chest fin base of female parent fish, and culturing the female and male parent fish in the pond at a ratio of 2-3:1, and performing uninterrupted running water stimulation;
(3) performing spawn collection and semen collection on the female and male parent fishes after the running water is stimulated for 10-11 hours;
(4) after induced spawning, the male and female parent fishes are put into natural water areas.
Further, in the step (2), the oxytocic pharmaceutical composition is formulated as follows: salmon analog 5ug/kg, chorionic gonadotropin 500IU/kg and diutanone maleate 1 ug/kg.
The beneficial technical effects of the invention are as follows: according to the scheme of the invention, fertilized eggs are obtained, and used wild hucho taimen parent fish can be reasonably and effectively protected, so that the technical problem that the fertilized eggs are difficult to obtain by domesticating the wild hucho taimen at the present stage can be solved, the protection of national class I protected animals can be promoted, and the ecological benefit is remarkable.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
An artificial induced spawning method for wild hucho taimen, comprising the following steps:
(1) when the water temperature of a growing water area of wild hucho taimen every year is 6.5 ℃, selecting male fishes with the weight of 5kg and female fishes with the weight of 7kg under natural growth, respectively counting the tails and weighing;
(2) the medicines are diluted respectively by using 5% glucose solution as a diluent and mixed for use.
(3) Injecting the oxytocic pharmaceutical composition at 1/3 below the chest fin base of female parent fish according to 0.4mL/kg, breeding the female parent fish and the male parent fish in the pool according to the ratio of 2:1 after injection, and performing uninterrupted running water stimulation;
the oxytocic pharmaceutical composition comprises the following components in percentage by weight: salmon analog 5ug/kg, chorionic gonadotropin 500IU/kg and domaleic acid diutanone 1 ug/kg.
(4) After the flowing water is stimulated for 10 hours, egg taking and sperm taking are respectively carried out on the female fish and the male fish;
(5) after the induction of labor is finished, the male fish and the female fish are put into a natural water area.
Example 2
An artificial induced spawning method for wild hucho taimen, comprising the following steps:
(1) when the water temperature of a growing water area of wild hucho taimen every year is 6.2 ℃, selecting male fishes with the weight of 4.5kg and female fishes with the weight of 6.6kg under natural growth, respectively counting the tails and weighing;
(2) the medicines are diluted respectively by using 5% glucose solution as a diluent and mixed for use.
(3) Injecting an oxytocic pharmaceutical composition at 1/3 below the chest fin base of female parent fish according to 0.4mL/kg, and culturing the female parent fish and the male parent fish in a pool according to a ratio of 3:1 after injection, and performing uninterrupted running water stimulation;
the oxytocic pharmaceutical composition comprises the following components in percentage by weight: salmon analog 5ug/kg, chorionic gonadotropin 500IU/kg and diutanone maleate 1ug/kg, and 5% glucose was used as a diluent during the pharmaceutical formulation.
(4) After the flowing water is stimulated for 11 hours, egg taking and sperm taking are respectively carried out on the female fish and the male fish;
(5) after the artificial fertilization is finished, the male fish and the female fish are put into a natural water area.
Example 3
An artificial induced spawning method for wild hucho taimen, comprising the following steps:
(1) when the water temperature of a growing water area of wild hucho taimen every year is 7.5 ℃, selecting male fishes with the weight of 5.5kg and female fishes with the weight of 7.5kg under natural growth, respectively counting the tails and weighing;
(2) the medicines are diluted respectively by using 5% glucose solution as a diluent and mixed for use.
(3) Injecting an oxytocic pharmaceutical composition at 1/3 below the chest fin base of female parent fish according to 0.4mL/kg, and culturing the female parent fish and the male parent fish in a pool according to a ratio of 3:1 after injection, and performing uninterrupted running water stimulation;
the oxytocic pharmaceutical composition comprises the following components in percentage by weight: salmon analog 5ug/kg, chorionic gonadotropin 500IU/kg and diutanone maleate 1ug/kg, and 5% glucose was used as a diluent during the pharmaceutical formulation. .
(4) After the flowing water is stimulated for 10 hours, egg taking and sperm taking are respectively carried out on the female fish and the male fish;
(5) after the artificial induced spawning is finished, the male fish and the female fish are put into a natural water area.
TABLE 1 induction recording table of the invention
It will be understood by those skilled in the art that, unless otherwise defined, all terms (including technical and scientific terms) used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. It will be further understood that terms, such as those defined in commonly used dictionaries, should be interpreted as having a meaning that is consistent with their meaning in the context of the prior art and will not be interpreted in an idealized or overly formal sense unless expressly so defined herein.
Finally, it should be noted that: although the present invention has been described in detail with reference to the above embodiments, it should be understood by those skilled in the art that: modifications and equivalents may be made thereto without departing from the spirit and scope of the invention and it is intended to cover in the claims the invention as defined in the appended claims.
Claims (1)
1. An artificial induced spawning method for wild hucho taimen is characterized by comprising the following steps:
(1) when the water temperature of a growing water area of wild hucho taimen every year is 6.2-7.5 ℃, selecting male fishes with the weight of more than 4kg and female fishes with the weight of more than 6.5kg under natural growth conditions, respectively counting the tails and weighing;
(2) injecting 0.4mL/kg of oxytocic pharmaceutical composition diluted by glucose with mass concentration of 5% into 1/3 below chest fin base of female parent fish, and culturing the female and male parent fish in the pond at a ratio of 2-3:1, and performing uninterrupted running water stimulation; the oxytocic pharmaceutical composition comprises the following components in parts by weight: salmon analog 5 μ g/kg, chorionic gonadotropin 500IU/kg and diutanone maleate 1 μ g/kg;
(3) performing spawn collection and semen collection on the female and male parent fishes after the running water is stimulated for 10-11 hours;
(4) after induced spawning, the male and female parent fishes are put into natural water areas.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710100206.5A CN106900604B (en) | 2017-02-23 | 2017-02-23 | Artificial induced spawning method for wild hucho taimen |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710100206.5A CN106900604B (en) | 2017-02-23 | 2017-02-23 | Artificial induced spawning method for wild hucho taimen |
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| Publication Number | Publication Date |
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| CN106900604A CN106900604A (en) | 2017-06-30 |
| CN106900604B true CN106900604B (en) | 2020-12-04 |
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| CN201710100206.5A Active CN106900604B (en) | 2017-02-23 | 2017-02-23 | Artificial induced spawning method for wild hucho taimen |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110612932A (en) * | 2019-11-05 | 2019-12-27 | 河北省海洋与水产科学研究院(河北省海洋渔业生态环境监测站) | Full-artificial propagation method of hucho taimen |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101317558B (en) * | 2008-06-16 | 2010-12-29 | 中国水产科学研究院黑龙江水产研究所 | Distant hybridization method for hucho trout and brachymystax lenok |
| CN103222436B (en) * | 2013-05-15 | 2014-07-09 | 中国水产科学研究院黑龙江水产研究所 | Artificial propagation method of esox reichertii |
| CN104904636B (en) * | 2015-06-10 | 2018-06-19 | 浙江省海洋水产研究所 | A kind of induced spawning method of little yellow croaker |
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