CN106841079B - 一种测定富含还原糖的蛋白样品中蛋白质含量的方法 - Google Patents
一种测定富含还原糖的蛋白样品中蛋白质含量的方法 Download PDFInfo
- Publication number
- CN106841079B CN106841079B CN201710213757.2A CN201710213757A CN106841079B CN 106841079 B CN106841079 B CN 106841079B CN 201710213757 A CN201710213757 A CN 201710213757A CN 106841079 B CN106841079 B CN 106841079B
- Authority
- CN
- China
- Prior art keywords
- protein
- reduced sugar
- glucose
- bca
- standard curve
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 235000000346 sugar Nutrition 0.000 title claims abstract description 83
- 238000000034 method Methods 0.000 title claims abstract description 82
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 81
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 81
- 238000005259 measurement Methods 0.000 title claims abstract description 23
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 25
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 71
- 239000008103 glucose Substances 0.000 claims description 70
- 239000007788 liquid Substances 0.000 claims description 36
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 30
- 239000000243 solution Substances 0.000 claims description 28
- 238000006243 chemical reaction Methods 0.000 claims description 21
- 239000012086 standard solution Substances 0.000 claims description 18
- 239000012153 distilled water Substances 0.000 claims description 16
- 108091003079 Bovine Serum Albumin Proteins 0.000 claims description 15
- 229940098773 bovine serum albumin Drugs 0.000 claims description 15
- 230000031700 light absorption Effects 0.000 claims description 12
- 238000002156 mixing Methods 0.000 claims description 10
- 239000000203 mixture Substances 0.000 claims description 10
- 238000009835 boiling Methods 0.000 claims description 9
- 239000006228 supernatant Substances 0.000 claims description 6
- 238000000227 grinding Methods 0.000 claims description 5
- 238000005119 centrifugation Methods 0.000 claims description 4
- 239000008363 phosphate buffer Substances 0.000 claims description 4
- 241000283690 Bos taurus Species 0.000 claims description 3
- 239000004570 mortar (masonry) Substances 0.000 claims description 3
- 210000002966 serum Anatomy 0.000 claims description 3
- 239000000523 sample Substances 0.000 description 66
- AFYNADDZULBEJA-UHFFFAOYSA-N bicinchoninic acid Chemical compound C1=CC=CC2=NC(C=3C=C(C4=CC=CC=C4N=3)C(=O)O)=CC(C(O)=O)=C21 AFYNADDZULBEJA-UHFFFAOYSA-N 0.000 description 54
- LWFUFLREGJMOIZ-UHFFFAOYSA-N 3,5-dinitrosalicylic acid Chemical compound OC(=O)C1=CC([N+]([O-])=O)=CC([N+]([O-])=O)=C1O LWFUFLREGJMOIZ-UHFFFAOYSA-N 0.000 description 20
- 206010018473 Glycosuria Diseases 0.000 description 13
- 241000208125 Nicotiana Species 0.000 description 12
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 12
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 9
- 238000002835 absorbance Methods 0.000 description 7
- 238000002360 preparation method Methods 0.000 description 7
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 5
- 210000002700 urine Anatomy 0.000 description 5
- 102000004506 Blood Proteins Human genes 0.000 description 4
- 108010017384 Blood Proteins Proteins 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 206010012601 diabetes mellitus Diseases 0.000 description 4
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 4
- 229910000403 monosodium phosphate Inorganic materials 0.000 description 4
- 235000019799 monosodium phosphate Nutrition 0.000 description 4
- 239000012452 mother liquor Substances 0.000 description 4
- 238000003556 assay Methods 0.000 description 3
- 239000012888 bovine serum Substances 0.000 description 3
- 238000012937 correction Methods 0.000 description 3
- 229910000397 disodium phosphate Inorganic materials 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 239000013589 supplement Substances 0.000 description 3
- JPVYNHNXODAKFH-UHFFFAOYSA-N Cu2+ Chemical compound [Cu+2] JPVYNHNXODAKFH-UHFFFAOYSA-N 0.000 description 2
- 101710138460 Leaf protein Proteins 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 235000019800 disodium phosphate Nutrition 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000002331 protein detection Methods 0.000 description 2
- 238000011002 quantification Methods 0.000 description 2
- 238000004445 quantitative analysis Methods 0.000 description 2
- 239000001488 sodium phosphate Substances 0.000 description 2
- 150000008163 sugars Chemical class 0.000 description 2
- MPDGHEJMBKOTSU-YKLVYJNSSA-N 18beta-glycyrrhetic acid Chemical compound C([C@H]1C2=CC(=O)[C@H]34)[C@@](C)(C(O)=O)CC[C@]1(C)CC[C@@]2(C)[C@]4(C)CC[C@@H]1[C@]3(C)CC[C@H](O)C1(C)C MPDGHEJMBKOTSU-YKLVYJNSSA-N 0.000 description 1
- 206010001580 Albuminuria Diseases 0.000 description 1
- 208000007342 Diabetic Nephropathies Diseases 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 206010023379 Ketoacidosis Diseases 0.000 description 1
- 208000007976 Ketosis Diseases 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 244000061458 Solanum melongena Species 0.000 description 1
- 235000009754 Vitis X bourquina Nutrition 0.000 description 1
- 235000012333 Vitis X labruscana Nutrition 0.000 description 1
- 240000006365 Vitis vinifera Species 0.000 description 1
- 235000014787 Vitis vinifera Nutrition 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 229940101006 anhydrous sodium sulfite Drugs 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000000337 buffer salt Substances 0.000 description 1
- 239000002738 chelating agent Substances 0.000 description 1
- 239000013065 commercial product Substances 0.000 description 1
- UGWKCNDTYUOTQZ-UHFFFAOYSA-N copper;sulfuric acid Chemical compound [Cu].OS(O)(=O)=O UGWKCNDTYUOTQZ-UHFFFAOYSA-N 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 208000033679 diabetic kidney disease Diseases 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- AUPXFICLXPLHBB-UHFFFAOYSA-L disodium;2-(4-carboxylatoquinolin-2-yl)quinoline-4-carboxylate Chemical compound [Na+].[Na+].C1=CC=CC2=NC(C=3C=C(C4=CC=CC=C4N=3)C(=O)[O-])=CC(C([O-])=O)=C21 AUPXFICLXPLHBB-UHFFFAOYSA-L 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005485 electric heating Methods 0.000 description 1
- 230000002641 glycemic effect Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 description 1
- 238000000703 high-speed centrifugation Methods 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- QLFFCLRSMTUBEZ-UHFFFAOYSA-N phosphoric acid;sodium Chemical compound [Na].[Na].OP(O)(O)=O QLFFCLRSMTUBEZ-UHFFFAOYSA-N 0.000 description 1
- VZOPRCCTKLAGPN-ZFJVMAEJSA-L potassium;sodium;(2r,3r)-2,3-dihydroxybutanedioate;tetrahydrate Chemical compound O.O.O.O.[Na+].[K+].[O-]C(=O)[C@H](O)[C@@H](O)C([O-])=O VZOPRCCTKLAGPN-ZFJVMAEJSA-L 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- HELHAJAZNSDZJO-OLXYHTOASA-L sodium L-tartrate Chemical compound [Na+].[Na+].[O-]C(=O)[C@H](O)[C@@H](O)C([O-])=O HELHAJAZNSDZJO-OLXYHTOASA-L 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- 229940074446 sodium potassium tartrate tetrahydrate Drugs 0.000 description 1
- GEHJYWRUCIMESM-UHFFFAOYSA-L sodium sulfite Chemical compound [Na+].[Na+].[O-]S([O-])=O GEHJYWRUCIMESM-UHFFFAOYSA-L 0.000 description 1
- 239000001433 sodium tartrate Substances 0.000 description 1
- 229960002167 sodium tartrate Drugs 0.000 description 1
- 235000011004 sodium tartrates Nutrition 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000002562 urinalysis Methods 0.000 description 1
- 230000002485 urinary effect Effects 0.000 description 1
- 208000019206 urinary tract infection Diseases 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 210000004885 white matter Anatomy 0.000 description 1
- 239000012224 working solution Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/33—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using ultraviolet light
Landscapes
- Physics & Mathematics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Description
Claims (5)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201710213757.2A CN106841079B (zh) | 2017-04-01 | 2017-04-01 | 一种测定富含还原糖的蛋白样品中蛋白质含量的方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201710213757.2A CN106841079B (zh) | 2017-04-01 | 2017-04-01 | 一种测定富含还原糖的蛋白样品中蛋白质含量的方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN106841079A CN106841079A (zh) | 2017-06-13 |
CN106841079B true CN106841079B (zh) | 2019-06-18 |
Family
ID=59142265
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201710213757.2A Active CN106841079B (zh) | 2017-04-01 | 2017-04-01 | 一种测定富含还原糖的蛋白样品中蛋白质含量的方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN106841079B (zh) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110988249B (zh) * | 2019-11-18 | 2021-01-29 | 福建农林大学 | 一种含有还原性单糖的溶液中水溶性花生蛋白质浓度的测定方法 |
CN111060459A (zh) * | 2019-12-17 | 2020-04-24 | 佛山科学技术学院 | 一种基于rgb色彩体系的食品中还原糖、蛋白质快速检测方法 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101946178A (zh) * | 2008-02-22 | 2011-01-12 | 欧雷恩诊断公司 | 检测被分析物的方法和装置 |
CN102608046A (zh) * | 2012-03-05 | 2012-07-25 | 熊鹏 | 简易的低聚木糖平均聚合度的测定方法 |
CN104897656A (zh) * | 2015-06-23 | 2015-09-09 | 川渝中烟工业有限责任公司 | 烟草蛋白质含量的测定方法 |
CN105004589A (zh) * | 2015-07-21 | 2015-10-28 | 中国科学技术大学 | 一种用蔗糖促进蛋白沉淀的方法 |
-
2017
- 2017-04-01 CN CN201710213757.2A patent/CN106841079B/zh active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101946178A (zh) * | 2008-02-22 | 2011-01-12 | 欧雷恩诊断公司 | 检测被分析物的方法和装置 |
CN102608046A (zh) * | 2012-03-05 | 2012-07-25 | 熊鹏 | 简易的低聚木糖平均聚合度的测定方法 |
CN104897656A (zh) * | 2015-06-23 | 2015-09-09 | 川渝中烟工业有限责任公司 | 烟草蛋白质含量的测定方法 |
CN105004589A (zh) * | 2015-07-21 | 2015-10-28 | 中国科学技术大学 | 一种用蔗糖促进蛋白沉淀的方法 |
Non-Patent Citations (7)
Title |
---|
A COMPARISON OF PROTEIN ASSAYS FOR THE DETERMINATION OF THE PROTEIN-CONCENTRATION OF BEER;WILLIAMS K M,FOX P et al;《JOURNAL OF THE INSTITUTE OF BREWING》;19951031;第101卷(第5期);365-369 |
BCA法测定微量蛋白质的初步评价;周祖钊,朱文雅等;《临床检验杂质》;19921231;第10卷(第3期);134-135 |
INTERFERENCE OF BIOGENIC-AMINES WITH THE MEASUREMENT OF PROTEINS USING BICINCHONINIC ACID;SLOCUM T L,DEUPREE J D et al;《ANALYTICAL BIOCHEMISTRY 》;19910515;第195卷(第1期);14-17 |
Measurement of low level membrane proteins using bicinchoninic acid: modified procedures to eliminate interfering substances;Shiwen Luo,Masako Takano et al.;《Fukuoka igaku zasshi = Hukuoka acta medica》;20021101;236-246 |
protein measurement using bicinchoninic acid:elimination of interfering substances;BROWN R E,JARVIS K L et al;《ANALYTICAL BIOCHEMISTRY》;19890731;第180卷(第1期);136-139 |
Quantitative Colorimetric Assay for Total Protein Applied to the Red Wine Pinot Noir;Mark R Smith,Mike H Penner et al;《JUOUNAL OF AGRICULTURE AND FOOD CHEMISTRY》;20111231;第59卷(第13期);6871-6876 |
还原糖和非还原糖对BCA法测定微量蛋白质的影响;刘家喜,夏寿扬;《上海医学检验杂质》;19941231;第9卷(第1期);34-35 |
Also Published As
Publication number | Publication date |
---|---|
CN106841079A (zh) | 2017-06-13 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Fischer et al. | A simple serum iron method using the new sensitive chromogen tripyridyl-s-triazine | |
Sathyanarayana et al. | Melamine and cyanuric acid exposure and kidney injury in US children | |
McCaman et al. | Fluorimetric method for the determination of phenylalanine in serum | |
Vikbladh | Studies on zinc in blood. I | |
CN103760357B (zh) | 一种缺血修饰白蛋白检测试剂盒 | |
CN102621332B (zh) | 基于胶乳粒子包被视黄醇结合蛋白检测试剂盒 | |
CN106841079B (zh) | 一种测定富含还原糖的蛋白样品中蛋白质含量的方法 | |
CN112540116B (zh) | 利用内标组合溶液检测全血中六种微量元素的方法 | |
Malon et al. | Ionized magnesium in erythrocytes—the best magnesium parameter to observe hypo-or hypermagnesemia | |
CN112540115A (zh) | 用于检测全血中6种单元素的内标组合溶液 | |
CN112557492A (zh) | 利用内标组合溶液来校准icp-ms微量元素分析仪的方法 | |
Saifer et al. | Photometric microdetermination of amino acids in biological fluids with the ninhydrin reaction | |
Purwati et al. | Correlation of serum nitric oxide and urine malondialdehyde levels in non-hemodialysis chronic kidney disease patients | |
CN105445409B (zh) | 一种利用同位素稀释质谱法测量糖化血红蛋白的方法 | |
CN108949902A (zh) | 一种血清小而密低密度脂蛋白胆固醇检测试剂盒 | |
CN102944683A (zh) | 双试剂法检测间接胆红素试剂盒及制备方法 | |
Cox et al. | The measurement of dehydroascorbic acid and diketogulonic acid in normal and diabetic plasma | |
CN111289501A (zh) | 一种基于间接比色法检测no的试剂盒及其制备使用方法 | |
CN103674938A (zh) | 缺血修饰白蛋白组合测定试剂、测定方法及试剂盒 | |
CN104380114B (zh) | Pc‑o 42:4-一种用于内脏脂肪过多的生物标志物 | |
EP1664771A2 (en) | A method for qualitative and/or quantitative detection of polyethylene glycols in biological fluids | |
Abdullah et al. | Determination of serum zinc, manganese, copper and cobalt traces in type two diabetic patients in Sulaimaniyah city using icp technique | |
CN106556595B (zh) | 一种抗干扰能力强的肌酐苦味酸法检测试剂盒 | |
Feitosa et al. | Selenium status and its relation to markers of oxidative stress in obese women | |
CN108152225B (zh) | 一种缺血修饰白蛋白的测定方法及检测试剂盒 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20210521 Address after: No.69 Hongguang Avenue, Banan District, Chongqing Patentee after: Xuzhou China Intellectual Property Service Co.,Ltd. Address before: No.69 Hongguang Avenue, Banan District, Chongqing Patentee before: Chongqing University of Technology |
|
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20210804 Address after: 239000 room 107, building 2, international science and technology innovation center, 1500 Hongwu East Road, Nanqiao District, Chuzhou City, Anhui Province Patentee after: Chuzhou Kaibo Biological Technology Co.,Ltd. Address before: No.69 Hongguang Avenue, Banan District, Chongqing Patentee before: Xuzhou China Intellectual Property Service Co.,Ltd. |