CN106832038A - A kind of extracting method of bletilla polysaccharide - Google Patents
A kind of extracting method of bletilla polysaccharide Download PDFInfo
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- CN106832038A CN106832038A CN201710204450.6A CN201710204450A CN106832038A CN 106832038 A CN106832038 A CN 106832038A CN 201710204450 A CN201710204450 A CN 201710204450A CN 106832038 A CN106832038 A CN 106832038A
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
- C08B37/0087—Glucomannans or galactomannans; Tara or tara gum, i.e. D-mannose and D-galactose units, e.g. from Cesalpinia spinosa; Tamarind gum, i.e. D-galactose, D-glucose and D-xylose units, e.g. from Tamarindus indica; Gum Arabic, i.e. L-arabinose, L-rhamnose, D-galactose and D-glucuronic acid units, e.g. from Acacia Senegal or Acacia Seyal; Derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids
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Abstract
The present invention relates to Chinese medicine extractive technique field, more particularly to a kind of extracting method of bletilla polysaccharide；Comprise the following steps：Hyacinth bletilla gel preparation, decolouring, deproteinized, purification；Using preparation method of the invention prepare bletilla polysaccharide, its purity up to more than 90%, by rational parameter setting so that the recovery rate of the bletilla striata improves 20.46~26.06% compared with conventional method.
Description
Technical field
The present invention relates to Chinese medicine extractive technique field, more particularly to a kind of extracting method of bletilla polysaccharide.
Background technology
The bletilla striata is the dry tuber that a kind of Chinese medicine of medication long history is the orchid bletilla striata, China《Compendium of Materia Medica》Have
Record：" bletilla striata alias even and grass, give free of charge, its root white by Radix Glycyrrhizae, even and and give birth to, therefore say the bletilla striata, most early in《Sheng Nong's herbal classic》
In it is on the books, to be concealed in each version after 1963《Chinese medicine pharmacopeia》In one of herbal species, the bletilla striata contains substantial amounts of
Mucilage, property is bitter, puckery, sweet, is slightly cold, and enters return lung, liver, stomach, there is that astringing to arrest bleeding, heat-clearing be wet, detumescence and promoting granulation.Clinically
Treatment hemoptysis, haematemesis, sore swollen toxin, traumatism and bleeding, chapped skin, pulmonary tuberculosis hemoptysis and ulcer haemorrhage etc. are widely used in, are treated
Effect is notable, and the main chemical compositions of the bletilla striata are bibenzyl, luxuriant and rich with fragrance class and its derivative, additionally, also containing a small amount of volatile oil, mucus
Matter, bletilla mannan and starch and glucose.
It is with β-glucosides by glucose and mannose (1: 3) containing abundant bletilla polysaccharide (Bletilla glucomannan) in bletilla striata medicinal material
A kind of glucomannans that key is polymerized, bletilla polysaccharide as natural macromolecular material, with functional slow-release, local retention
Property, itself degradability, it is non-stimulated, have no toxic side effect, be increasingly taken seriously in medicinal preparation process, mainly have as medicine
Thing carrier, bletilla polysaccharide belongs to natural small molecule polymer, can be used as the capsule material of micro-capsule, the matrix of suppository, the adhesive of tablet, sugar
The insolated layer materials of garment piece, film filmogen, it is also possible to as coupling agent for medical use, also developed in addition based on bletilla polysaccharide
Want the dextran product of raw material；Bletilla polysaccharide can be added because of its unique performance, and with security higher as food
Agent, is widely used in food industry, using the excellent film forming of bletilla polysaccharide, can prepare the antistaling agent of fruit film, can
To reduce fruit moisture evaporation, breathed after suppressing to pluck, to reach the purpose of extension insurance, while bletilla polysaccharide can be formed
High and low concentration type gel, similar to gum arabic, antiacid, alkali excellent performance can be used as the stabilizer in emulsion or increasing for performance
Thick dose, bletilla polysaccharide is a kind of preferable cosmetic additive agent while being also active component, and it has convergence to skin, delays to decline
Always, thickening, moisturizing, the effects such as help emulsification.Add bletilla polysaccharide cosmetics can effectively anti-frostbite be chapped from the cold, promote blood to follow
Ring, moisture-keeping crease-shedding, and have no toxic side effect, someone is developed with bletilla polysaccharide as the cosmetics of main active substances, is had
Bactericidal antiphlogistic, freckle removing and whitening, the function of crease-resistant health care, can industrially make thickener, starch silk and cotton yarn, or coating.
The bletilla striata has stronger health-care effect and economic worth, deep to receive the favor of people and pursue, but now in the bletilla striata
Processing on be mostly the roughing for carrying out some bletilla striatas, do not develop the health value of the bletilla striata completely, simultaneously as plus
Technical deficiency in work method, causes to destroy the nutritional ingredient and structure of the bletilla striata in process, the serious reduction bletilla striata
Health properties, therefore deep processing is carried out to the bletilla striata, such as to the exploitation of bletilla polysaccharide be have important practical significance and
Economic implications, while also for the field of deep of Chinese medicine provides a kind of new direction.
The content of the invention
The present invention is in order to solve the above technical problems, there is provided a kind of extracting method of bletilla polysaccharide.
Realized particular by following technical scheme：
A kind of extracting method of bletilla polysaccharide, comprises the following steps：
(1) prepared by hyacinth bletilla gel：The bletilla striata is crushed, is added water, 120~150min is stirred in magnetic stirring apparatus, temperature is dropped
To 60~70 DEG C, constant temperature stirring adds ethanol with the speed of 2~3 drop/s, stirs 35~45min, filters while hot, obtains the bletilla striata and coagulates
Glue；
(2) decolouring, deproteinized：Hydrogen peroxide is added in hyacinth bletilla gel, after washing three times with hot ethanol, heating separates dioxygen
Water, filtering, adds activated carbon in filtrate, filters, and hydrochloric acid is added in filtrate, is heated to 75~85 DEG C, and stirring 20~
30min, adds NaOH, and regulation solution pH value is 6~6.5, obtains material 1；
(3) purify：Will material 1 with 20~30min of ultrasonic wave extraction, then with 8~12min of CO2 means of supercritical extraction, you can.
Described magnetic stirring apparatus, its whipping temp is 90~110 DEG C.
Described ethanol, its concentration >=70%.
Described hydrogen peroxide, its addition is the 5~8% of hyacinth bletilla gel quality.
The activated carbon, its addition is the 8~12% of hyacinth bletilla gel quality.
Described hydrochloric acid, its addition is the 20~30% of hyacinth bletilla gel quality, and its concentration is 3.8~4.2%.
Described NaOH, its concentration is 1.5~2%.
Described ultrasonic wave extraction, its supersonic frequency is 30~40KHz.
The extracting method of described bletilla polysaccharide, after the bletilla striata is crushed, also including entering line activating treatment to common bletilla, be
With 70~80 DEG C of steam to the bletilla striata 15~20min of powder-processed, then common bletilla, cellulase and pectase are pressed 2: 0.08
~0.12: 0.2~0.3 mass ratio mixing, after mixing thoroughly, after standing 24h, then the preparation for carrying out hyacinth bletilla gel.
In sum, the beneficial effects of the present invention are：The bletilla polysaccharide prepared using preparation method of the invention, its is pure
Degree up to more than 90%, by rational parameter setting so that the recovery rate of the bletilla striata improves 20.46 compared with conventional method~
26.06%.
Specific embodiment
Specific embodiment of the invention is described in further detail below, but the invention is not limited in these realities
Mode is applied, any improvement or replacement on the present embodiment essence spirit still falls within the claims in the present invention required for protection
Scope.
Embodiment 1
A kind of extracting method of bletilla polysaccharide, comprises the following steps：
(1) prepared by hyacinth bletilla gel：The bletilla striata is crushed, with 70 DEG C of steam to bletilla striata powder-processed 15min, then by bletilla striata powder
End, cellulase and pectase are mixed by 2: 0.08: 0.2 mass ratio, after mixing thoroughly, after standing 24h, are added water, in 90 DEG C of magnetic
120min is stirred in power agitator, 60 DEG C are cooled the temperature to, constant temperature stirring adds ethanol, stirring with the speed of 2~3 drop/s
35min, filters while hot, obtains hyacinth bletilla gel；
(2) decolouring, deproteinized：Hydrogen peroxide is added in hyacinth bletilla gel, its addition is the 5% of hyacinth bletilla gel quality, used
After hot ethanol washs three times, heating separates hydrogen peroxide, and filtering adds activated carbon in filtrate, and its addition is hyacinth bletilla gel matter
The 8% of amount, filtering adds the hydrochloric acid that concentration is 3.8% in filtrate, and addition is the 20% of hyacinth bletilla gel quality, is heated to
75 DEG C, 20min is stirred, add the NaOH that concentration is 1.5%, regulation solution pH value is 6, obtains material 1；
(3) purify：Will material 1 with the ultrasonic wave extraction 20min that frequency is 30KHz, then with CO2 means of supercritical extraction 8min, i.e.,
Can.
Embodiment 2
A kind of extracting method of bletilla polysaccharide, comprises the following steps：
(1) prepared by hyacinth bletilla gel：The bletilla striata is crushed, with 80 DEG C of steam to bletilla striata powder-processed 20min, then by bletilla striata powder
End, cellulase and pectase are mixed by 2: 0.12: 0.3 mass ratio, after mixing thoroughly, after standing 24h, are added water, in 110 DEG C of magnetic
150min is stirred in power agitator, 70 DEG C are cooled the temperature to, constant temperature stirring adds ethanol, stirring with the speed of 2~3 drop/s
45min, filters while hot, obtains hyacinth bletilla gel；
(2) decolouring, deproteinized：Hydrogen peroxide is added in hyacinth bletilla gel, its addition is the 8% of hyacinth bletilla gel quality, used
After hot ethanol washs three times, heating separates hydrogen peroxide, and filtering adds activated carbon in filtrate, and its addition is hyacinth bletilla gel matter
12% filtering of amount, adds the hydrochloric acid that concentration is 4.2% in filtrate, and addition is the 30% of hyacinth bletilla gel quality, is heated to
85 DEG C, 30min is stirred, add the NaOH that concentration is 2%, regulation solution pH value is 6.5, obtains material 1；
(3) purify：Will material 1 with the ultrasonic wave extraction 30min that frequency is 40KHz, then with CO2 means of supercritical extraction 12min, i.e.,
Can.
Embodiment 3
A kind of extracting method of bletilla polysaccharide, comprises the following steps：
(1) prepared by hyacinth bletilla gel：The bletilla striata is crushed, with 75 DEG C of steam to bletilla striata powder-processed 18min, then by bletilla striata powder
End, cellulase and pectase are mixed by 2: 0.01: 0.25 mass ratio, after mixing thoroughly, after standing 24h, are added water, 90~110
DEG C magnetic stirring apparatus in stir 135min, cool the temperature to 65 DEG C, constant temperature stirring, with the speed addition ethanol of 2~3 drop/s,
Stirring 40min, filters while hot, obtains hyacinth bletilla gel；
(2) decolouring, deproteinized：Hydrogen peroxide is added in hyacinth bletilla gel, its addition is the 7% of hyacinth bletilla gel quality, used
After hot ethanol washs three times, heating separates hydrogen peroxide, and filtering adds activated carbon in filtrate, and its addition is hyacinth bletilla gel matter
10% filtering of amount, adds the hydrochloric acid that concentration is 4% in filtrate, and addition is the 25% of hyacinth bletilla gel quality, is heated to 80
DEG C, 25min is stirred, the NaOH that concentration is 1.8% is added, regulation solution pH value is 6, obtains material 1；
(3) purify：Will material 1 with the ultrasonic wave extraction 25min that frequency is 35KHz, then with CO2 means of supercritical extraction 10min, i.e.,
Can.
Embodiment 4
A kind of extracting method of bletilla polysaccharide, comprises the following steps：
(1) prepared by hyacinth bletilla gel：The bletilla striata is crushed, with 70 DEG C of steam to bletilla striata powder-processed 15min, then by bletilla striata powder
End, cellulase and pectase are mixed by 2: 0.12: 0.3 mass ratio, after mixing thoroughly, after standing 24h, are added water, in 110 DEG C of magnetic
120min is stirred in power agitator, 60 DEG C are cooled the temperature to, constant temperature stirring adds ethanol, stirring with the speed of 2~3 drop/s
45min, filters while hot, obtains hyacinth bletilla gel；
(2) decolouring, deproteinized：Hydrogen peroxide is added in hyacinth bletilla gel, its addition is the 5% of hyacinth bletilla gel quality, used
After hot ethanol washs three times, heating separates hydrogen peroxide, and filtering adds activated carbon in filtrate, and its addition is hyacinth bletilla gel matter
12% filtering of amount, adds the hydrochloric acid that concentration is 3.8% in filtrate, and addition is the 20% of hyacinth bletilla gel quality, is heated to
85 DEG C, 20min is stirred, add the NaOH that concentration is 2%, regulation solution pH value is 6, obtains material 1；
(3) purify：Will material 1 with the ultrasonic wave extraction 30min that frequency is 30KHz, then with CO2 means of supercritical extraction 8~
12min, you can.
Embodiment 5
A kind of extracting method of bletilla polysaccharide, comprises the following steps：
(1) prepared by hyacinth bletilla gel：The bletilla striata is crushed, with 80 DEG C of steam to bletilla striata powder-processed 20min, then by bletilla striata powder
End, cellulase and pectase are mixed by 2: 0.12: 0.2 mass ratio, after mixing thoroughly, after standing 24h, are added water, in 90 DEG C of magnetic
150min is stirred in power agitator, 60 DEG C are cooled the temperature to, constant temperature stirring adds ethanol, stirring with the speed of 2~3 drop/s
35min, filters while hot, obtains hyacinth bletilla gel；
(2) decolouring, deproteinized：Hydrogen peroxide is added in hyacinth bletilla gel, its addition is the 8% of hyacinth bletilla gel quality, used
After hot ethanol washs three times, heating separates hydrogen peroxide, and filtering adds activated carbon in filtrate, and its addition is hyacinth bletilla gel matter
8% filtering of amount, adds the hydrochloric acid that concentration is 3.8% in filtrate, and addition is the 20% of hyacinth bletilla gel quality, is heated to 85
DEG C, 20min is stirred, the NaOH that concentration is 1.5% is added, regulation solution pH value is 6.5, obtains material 1；
(3) purify：Will material 1 with the ultrasonic wave extraction 30min that frequency is 30KHz, then with CO2 means of supercritical extraction 8min, i.e.,
Can.
Experimental example
The purity and recovery rate of the bletilla polysaccharide prepared to the present invention are counted, with the extraction of traditional bletilla polysaccharide
Method as control, as a result as shown in table 1：
Table 1
| Project | Purity % | Recovery rate % |
| Embodiment 1 | 92.3 | 34.12 |
| Embodiment 2 | 91.8 | 33.56 |
| Embodiment 3 | 92.5 | 35.12 |
| Embodiment 4 | 92.4 | 35.02 |
| Embodiment 5 | 91.9 | 34.63 |
| Control group | 88.6 | 27.86 |
Explanation：The bletilla polysaccharide prepared using preparation method of the invention, its purity is joined up to more than 90% by rational
Number is set so that the recovery rate lifting 20.46~26.06% of the bletilla striata.
Claims (8)
1. a kind of extracting method of bletilla polysaccharide, it is characterised in that comprise the following steps：
(1) prepared by hyacinth bletilla gel：The bletilla striata is crushed, is added water, 120~150min is stirred in magnetic stirring apparatus, cool the temperature to 60
~70 DEG C, constant temperature stirring adds ethanol with the speed of 2~3 drop/s, stirs 35~45min, filters while hot, obtains hyacinth bletilla gel；
(2) decolouring, deproteinized：Hydrogen peroxide is added in hyacinth bletilla gel, after washing three times with hot ethanol, heating separates hydrogen peroxide,
Filtering, adds activated carbon in filtrate, filters, and hydrochloric acid is added in filtrate, is heated to 75~85 DEG C, stirs 20~30min, plus
Enter NaOH, regulation solution pH value is 6~6.5, obtains material 1；
(3) purify：Will material 1 with 20~30min of ultrasonic wave extraction, then with 8~12min of CO2 means of supercritical extraction, you can.
2. the extracting method of bletilla polysaccharide as claimed in claim 1, it is characterised in that described magnetic stirring apparatus, its stirring
Temperature is 90~110 DEG C.
3. the extracting method of bletilla polysaccharide as claimed in claim 1, it is characterised in that described ethanol, its concentration >=70%.
4. the extracting method of bletilla polysaccharide as claimed in claim 1, it is characterised in that described hydrogen peroxide, its addition is
The 5~8% of hyacinth bletilla gel quality.
5. the extracting method of bletilla polysaccharide as claimed in claim 1, it is characterised in that the activated carbon, its addition is white
The 8~12% of splendid achnatherum gel quality.
6. the extracting method of bletilla polysaccharide as claimed in claim 1, it is characterised in that described hydrochloric acid, its addition is white
The 20~30% of splendid achnatherum gel quality, its concentration is 3.8~4.2%.
7. the extracting method of bletilla polysaccharide as claimed in claim 1, it is characterised in that described ultrasonic wave extraction, its ultrasound
Frequency is 30~40KHz.
8. the extracting method of bletilla polysaccharide as claimed in claim 1, it is characterised in that after the bletilla striata is crushed, also including dialogue
Splendid achnatherum powder enters line activating treatment, be the steam with 70~80 DEG C to the bletilla striata 15~20min of powder-processed, then by common bletilla, fibre
The plain enzyme of dimension and pectase are mixed by 2: 0.08~0.12: 0.2~0.3 mass ratio, after mixing thoroughly, after standing 24h, then carry out the bletilla striata
The preparation of gel.
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Cited By (7)
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| CN107501434A (en) * | 2017-09-29 | 2017-12-22 | 贵州同源中药发展有限公司 | A kind of bletilla striata polyoses glue extracting method |
| CN108456611A (en) * | 2018-04-13 | 2018-08-28 | 宁夏悦丰生态农业科技有限公司 | Bletilla striata wine and preparation method thereof |
| CN109666083A (en) * | 2019-01-31 | 2019-04-23 | 贵州盛茂白芨开发有限公司 | A kind of plant polyose extraction element and its method for extracting bletilla polysaccharide |
| CN110684812A (en) * | 2019-11-15 | 2020-01-14 | 贵州省中国科学院天然产物化学重点实验室(贵州医科大学天然产物化学重点实验室) | Bletilla striata polysaccharide with immunocompetence and preparation method and application thereof |
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| CN116178586A (en) * | 2023-03-30 | 2023-05-30 | 金创克(珠海)生物技术有限公司 | Application of bletilla striata polysaccharide in preparation of product with moisturizing effect |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107501434A (en) * | 2017-09-29 | 2017-12-22 | 贵州同源中药发展有限公司 | A kind of bletilla striata polyoses glue extracting method |
| CN108456611A (en) * | 2018-04-13 | 2018-08-28 | 宁夏悦丰生态农业科技有限公司 | Bletilla striata wine and preparation method thereof |
| CN109666083A (en) * | 2019-01-31 | 2019-04-23 | 贵州盛茂白芨开发有限公司 | A kind of plant polyose extraction element and its method for extracting bletilla polysaccharide |
| CN109666083B (en) * | 2019-01-31 | 2021-06-04 | 贵州盛茂白芨开发有限公司 | Plant polysaccharide extraction device and method for extracting bletilla striata polysaccharide by using plant polysaccharide extraction device |
| CN110684812A (en) * | 2019-11-15 | 2020-01-14 | 贵州省中国科学院天然产物化学重点实验室(贵州医科大学天然产物化学重点实验室) | Bletilla striata polysaccharide with immunocompetence and preparation method and application thereof |
| TWI767559B (en) * | 2021-02-09 | 2022-06-11 | 英屬維爾京群島商克麗緹娜智慧產權有限公司 | Extract of white and dedifferentiated cells and uses thereof and cosmetic products containing the same |
| US20220249360A1 (en) * | 2021-02-09 | 2022-08-11 | Chlitina Intelligence Limited | Dedifferentiated cell extract of Bletilla and method for whitening, anti-oxidation and anti-wrinkling applying the same, and cosmetic comprising the same |
| CN114028316A (en) * | 2021-03-31 | 2022-02-11 | 英属维京群岛商克丽缇娜智慧产权有限公司 | Bletilla striata dedifferentiated cell extract, application thereof and cosmetic containing same |
| CN114028316B (en) * | 2021-03-31 | 2024-06-18 | 英属维京群岛商克丽缇娜智慧产权有限公司 | Cosmetic comprising bletilla striata dedifferentiated cell extract and use thereof |
| CN116178586A (en) * | 2023-03-30 | 2023-05-30 | 金创克(珠海)生物技术有限公司 | Application of bletilla striata polysaccharide in preparation of product with moisturizing effect |
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