CN103044560A - Preparation method of bletilla striata polysaccharide - Google Patents
Preparation method of bletilla striata polysaccharide Download PDFInfo
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- CN103044560A CN103044560A CN 201110305379 CN201110305379A CN103044560A CN 103044560 A CN103044560 A CN 103044560A CN 201110305379 CN201110305379 CN 201110305379 CN 201110305379 A CN201110305379 A CN 201110305379A CN 103044560 A CN103044560 A CN 103044560A
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Abstract
A preparation method of bletilla striata polysaccharide belongs to a field of food processing, and concretely relates to the preparation method of the bletilla striata polysaccharide. The invention provides a method for preparing simple, high-purity and good-viscosity bletilla striata polysaccharide. Preparation steps comprises: 1, extraction of crude bletilla striata polysaccharide, comprising taking 200 grams of bletilla striata tuber, crushing, sieving with a 20-mesh sieve, adding ethanol with a mass volume of 70-90 % with an amount being 2 to 4 times of the amount of the bletilla striata tuber, immersing for 4 hours, draining the ethanol, adding deionized water with an amount being 10 to 20 times of the quality of the raw materials to extract under a temperature of 60 to 80 DEG C, stirring and immersing for 3 to 4 hours, filtering to separate slag, and centrifuging a filtrate under 8000 r/min for 15 minutes; and 2, refining of the bletilla striata polysaccharide with enzyme, comprising adding 1 to 12 mg/g of papain to hydrolyze the crude bletilla striata polysaccharide with a concentration of 2 to 5 %, with a pH value of the hydrolysis system being 5.5 to 8.5, a hydrolysis temperature being 40 to 60 DEG C and a hydrolysis time being 30 to 240 minutes, adding 95 % ethanol with an amount of 3 times of the volume of the hydrolysate into the hydrolysate after the enzymatic hydrolysis, standing and precipitating for 5 hours in a 4 DEG C refrigerator, and filtering.
Description
Technical field:
The invention belongs to food processing field, more particularly, relate to a kind of preparation method of BSP.
Background technology:
The bletilla striata is that the orchid family bletilla striata belongs to, claim again lotus and grass, snow such as end, it is the natural resources of Chinese medicinal materials of dietotherapeutic, " must be puckery and receive, enter the lung hemostasis, myogenic is controlled sore ", effect with astringing to arrest bleeding, clearing heat and promoting diuresis, detumescence and promoting granulation, clinically be mainly used in treating lung and hinder hemoptysis, surgical wound, the diseases such as stomach and digestive tract ulcer.Its underground bulb contains the water-soluble polysaccharide more than 30%, and main component is konjac glucomanna, also is the main function composition of Bletilla glucomannan, and the ratio of its seminose and two kinds of glycosyls of glucose is approximately 4: 1.BSP is a kind of good natural thickener and emulsion stabilizer, studies show that security is higher, and the emulsification compatibility is good.Because of its good biocompatibility and degradability, can be used as various forms of medicine carrying dressing repair materials, the embedding of functional substance, tissue and infilling work material; And carry out crosslinkedly with other natural polymer biomaterials, develop the new sustained releases such as intelligent hydrogels, the pharmaceutical film control medicine of environment sensitive.BSP also is widely used in modern traditional Chinese medicine and daily use chemicals industry, and especially its good water retention property and significant whitening function more and more are subjected to the favor of cosmetic industry.
The conventional medical bletilla striata is the Crude polysaccharides goods, adopt high temperature boiling water to decoct or potass extraction, and by the ethanol precipitation, drying makes bletilla striata konjac glucomanna.Thick BSP purity generally is lower than 80%, and color and luster is slightly yellow, and with flavour of a drug, also need make with extra care as additive or medical material.And early-stage Study shows, the separation methods such as solvent wash, decolouring, chromatograph, and effect is undesirable, and the BSP viscosity is large, and a lot of process operation difficulty are large.
Summary of the invention:
The present invention is exactly for the problems referred to above, and a kind of preparation method who prepares the BSP simple, that purity is high, viscosity is good is provided.
In order to realize above-mentioned purpose of the present invention, preparation process of the present invention is:
1. the extraction of bletilla striata Crude polysaccharides
Get bletilla striata stem tuber 200g, pulverize, cross 20 mesh sieves, add its 2～4 times of quality volumes and be 70～90% alcohol immersion 4h, after draining ethanol, 10～20 times of deionized waters that add again raw materials quality extract, 60～80 ℃ of temperature, stirring and leaching 3～4h, filtering separation filter residue, the centrifugal 15min of filtrate 8000r/min, supernatant liquor is concentrated into 1/3 of original volume through Rotary Evaporators, adds 80～95% ethanol by 2～4 times of its volumes, and 4～8 ℃ staticly settle 8h, filter, wash to get bletilla striata Crude polysaccharides, preservation is for subsequent use after the vacuum-drying;
2. enzyme process is made with extra care BSP
Bletilla striata Crude polysaccharides concentration is 2～5%, adding papoid 1～12mg/g is hydrolyzed, the pH of hydrolyzation system is 5.5～8.5, hydrolysis temperature is 40～60 ℃, hydrolysis time is 30～240min, adds 95% ethanol of 3 times of volumes after enzymolysis is finished in hydrolyzed solution, staticly settles 5h in 4 ℃ of refrigerators, filter, and precipitate with 75% washing with alcohol.
Beneficial effect of the present invention:
1. the present invention is to the extraction rate reached to 92% of the bletilla striata, and purity is high, impurity is less;
2. simple to operate, the process economy of the present invention is convenient to amplify and is produced.
Description of drawings:
Fig. 1, Fig. 2, Fig. 3, Fig. 4 are that three factors are on the impact of bletilla striata polysaccharide extract rate.
Embodiment:
The present invention utilizes enzyme hydrolysis method that the BSP in the pawpaw is prepared, and preparation process of the present invention is:
1. the extraction of bletilla striata Crude polysaccharides
Get bletilla striata stem tuber 200g, pulverize, cross 20 mesh sieves, add its 2～4 times of quality volumes and be 70～90% alcohol immersion 4h, after draining ethanol, 10～20 times of deionized waters that add again raw materials quality extract, 60～80 ℃ of temperature, stirring and leaching 3～4h, filtering separation filter residue, the centrifugal 15min of filtrate 8000r/min, supernatant liquor is concentrated into 1/3 of original volume through Rotary Evaporators, adds 80～95% ethanol by 2～4 times of its volumes, and 4～8 ℃ staticly settle 8h, filter, wash to get bletilla striata Crude polysaccharides, preservation is for subsequent use after the vacuum-drying;
2. enzyme process is made with extra care BSP
Bletilla striata Crude polysaccharides concentration is 2～5%, adding papoid 1～12mg/g is hydrolyzed, the pH of hydrolyzation system is 5.5～8.5, hydrolysis temperature is 40～60 ℃, hydrolysis time is 30～240min, adds 95% ethanol of 3 times of volumes after enzymolysis is finished in hydrolyzed solution, staticly settles 5h in 4 ℃ of refrigerators, filter, and precipitate with 75% washing with alcohol.
The present invention selects enzyme concentration, pH value, hydrolysis temperature, hydrolysis time as the influence factor that affects the BSP extraction yield, visible Fig. 1, Fig. 2, Fig. 3, Fig. 4.
The mensuration of BSP content adopts the phenol sulfuric acid process.
As a kind of preferred version, processing condition of the present invention are: enzyme concentration 8～10mg/g, and pH=7.0～7.5, extracting temperature is 50 ℃, enzymolysis time is 120～150 ℃.
Claims (2)
1. the preparation method of a BSP is characterized in that, preparation process of the present invention is:
(1) extraction of bletilla striata Crude polysaccharides
Get bletilla striata stem tuber 200g, pulverize, cross 20 mesh sieves, add its 2～4 times of quality volumes and be 70～90% alcohol immersion 4h, after draining ethanol, 10～20 times of deionized waters that add again raw materials quality extract, 60～80 ℃ of temperature, stirring and leaching 3～4h, filtering separation filter residue, the centrifugal 15min of filtrate 8000r/min, supernatant liquor is concentrated into 1/3 of original volume through Rotary Evaporators, adds 80～95% ethanol by 2～4 times of its volumes, and 4～8 ℃ staticly settle 8h, filter, wash to get bletilla striata Crude polysaccharides, preservation is for subsequent use after the vacuum-drying;
(2) the refining BSP of enzyme process
Bletilla striata Crude polysaccharides concentration is 2～5%, adding papoid 1～12mg/g is hydrolyzed, the pH of hydrolyzation system is 5.5～8.5, hydrolysis temperature is 40～60 ℃, hydrolysis time is 30～240min, adds 95% ethanol of 3 times of volumes after enzymolysis is finished in hydrolyzed solution, staticly settles 5h in 4 ℃ of refrigerators, filter, and precipitate with 75% washing with alcohol.
2. the preparation method of a kind of BSP according to claim 1 is characterized in that, processing condition of the present invention are: enzyme concentration 8～10mg/g, and pH=7.0～7.5, extracting temperature is 50 ℃, enzymolysis time is 120～150 ℃.
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| CN 201110305379 CN103044560A (en) | 2011-10-11 | 2011-10-11 | Preparation method of bletilla striata polysaccharide |
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| CN 201110305379 CN103044560A (en) | 2011-10-11 | 2011-10-11 | Preparation method of bletilla striata polysaccharide |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103665400A (en) * | 2013-11-12 | 2014-03-26 | 唐忠海 | Preparation method and application of hyacinth bletilla hemostatic gel |
| CN105919841A (en) * | 2016-02-04 | 2016-09-07 | 张家界本草科技有限公司 | Application of rhizoma bletillae in cosmetic whitening products |
| CN106397627A (en) * | 2016-11-24 | 2017-02-15 | 贵州盛茂白芨开发有限公司 | Gum bletilla medium addition extraction method |
| CN106632718A (en) * | 2016-12-28 | 2017-05-10 | 西安医学院 | Preparation method for rhizoma bletillae polysaccharide |
| CN106832038A (en) * | 2017-03-30 | 2017-06-13 | 镇远县道益堂生物科技有限公司 | A kind of extracting method of bletilla polysaccharide |
| CN106893000A (en) * | 2017-03-16 | 2017-06-27 | 云南龙发制药股份有限公司 | A kind of Bletilla glucomannan extracting method |
| CN107496977A (en) * | 2017-09-20 | 2017-12-22 | 云南世纪天元生物科技有限公司 | A kind of liquid dressing prepared with pure natural plant extract and preparation method thereof |
| CN111004335A (en) * | 2019-11-29 | 2020-04-14 | 湖南绿蔓生物科技股份有限公司 | Bletilla striata oligosaccharide extracted from rhizome of bletilla striata and preparation method thereof |
| CN111803577A (en) * | 2020-06-22 | 2020-10-23 | 庆元县乾宁道地药材有限公司 | Preparation process of bletilla striata gum compound |
| CN113304088A (en) * | 2021-05-26 | 2021-08-27 | 上海辉文生物技术股份有限公司 | Preparation method of bletilla striata extract with repairing and whitening effects and bletilla striata extract |
-
2011
- 2011-10-11 CN CN 201110305379 patent/CN103044560A/en active Pending
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103665400B (en) * | 2013-11-12 | 2016-01-13 | 张家界本草科技有限公司 | The preparation method of hyacinth bletilla hemostatic gel and application |
| CN103665400A (en) * | 2013-11-12 | 2014-03-26 | 唐忠海 | Preparation method and application of hyacinth bletilla hemostatic gel |
| CN105919841B (en) * | 2016-02-04 | 2018-09-18 | 张家界圣君中药材开发有限公司 | Application of the bletilla in beautifying whitening product |
| CN105919841A (en) * | 2016-02-04 | 2016-09-07 | 张家界本草科技有限公司 | Application of rhizoma bletillae in cosmetic whitening products |
| CN106397627A (en) * | 2016-11-24 | 2017-02-15 | 贵州盛茂白芨开发有限公司 | Gum bletilla medium addition extraction method |
| CN106632718B (en) * | 2016-12-28 | 2019-04-19 | 西安医学院 | The preparation method of bletilla polysaccharide |
| CN106632718A (en) * | 2016-12-28 | 2017-05-10 | 西安医学院 | Preparation method for rhizoma bletillae polysaccharide |
| CN106893000A (en) * | 2017-03-16 | 2017-06-27 | 云南龙发制药股份有限公司 | A kind of Bletilla glucomannan extracting method |
| CN106832038A (en) * | 2017-03-30 | 2017-06-13 | 镇远县道益堂生物科技有限公司 | A kind of extracting method of bletilla polysaccharide |
| CN107496977A (en) * | 2017-09-20 | 2017-12-22 | 云南世纪天元生物科技有限公司 | A kind of liquid dressing prepared with pure natural plant extract and preparation method thereof |
| CN107496977B (en) * | 2017-09-20 | 2020-12-01 | 北京紫藤医创医药信息技术有限公司 | Liquid dressing prepared from pure natural plant extract and preparation method thereof |
| CN111004335A (en) * | 2019-11-29 | 2020-04-14 | 湖南绿蔓生物科技股份有限公司 | Bletilla striata oligosaccharide extracted from rhizome of bletilla striata and preparation method thereof |
| CN111803577A (en) * | 2020-06-22 | 2020-10-23 | 庆元县乾宁道地药材有限公司 | Preparation process of bletilla striata gum compound |
| CN113304088A (en) * | 2021-05-26 | 2021-08-27 | 上海辉文生物技术股份有限公司 | Preparation method of bletilla striata extract with repairing and whitening effects and bletilla striata extract |
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Application publication date: 20130417 |