CN106893000A - A kind of Bletilla glucomannan extracting method - Google Patents
A kind of Bletilla glucomannan extracting method Download PDFInfo
- Publication number
- CN106893000A CN106893000A CN201710155280.7A CN201710155280A CN106893000A CN 106893000 A CN106893000 A CN 106893000A CN 201710155280 A CN201710155280 A CN 201710155280A CN 106893000 A CN106893000 A CN 106893000A
- Authority
- CN
- China
- Prior art keywords
- bletilla
- bletilla glucomannan
- precipitation
- extracting method
- glucomannan
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
- C08B37/0087—Glucomannans or galactomannans; Tara or tara gum, i.e. D-mannose and D-galactose units, e.g. from Cesalpinia spinosa; Tamarind gum, i.e. D-galactose, D-glucose and D-xylose units, e.g. from Tamarindus indica; Gum Arabic, i.e. L-arabinose, L-rhamnose, D-galactose and D-glucuronic acid units, e.g. from Acacia Senegal or Acacia Seyal; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Emergency Medicine (AREA)
- Sustainable Development (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a kind of Bletilla glucomannan extracting method, the method digests centrifugation alcohol precipitation macroporous absorption ion exchange absorption of decolourizing and dehydrates step by immersion section hot water return extraction, it is obviously improved the recovery rate and purity of Bletilla glucomannan, not in contact with other organic solvents in extraction process, extraction process is relatively easy, workable, large-scale production can be realized, product is odorless, tasteless powder, and purity is high, can be widely used in food production processing, medicine and daily chemical products field.
Description
Technical field
The invention belongs to field of medicine and chemical technology, and in particular to a kind of Bletilla glucomannan extraction process.
Background technology
The bletilla striata（Bletilla striata）, belong to perennial herb bulbous plant（Root tuber）, the traditional Chinese medical science thinks bletilla striata bitter, sweet
And puckery, cold nature, enter lung channel.Function with hemostasis, detumescence, can treat raw sore bleeding, swollen ulcer drug, ulcer, rhagadia manus et pedis.
《Weinan book on Chinese herbal medicine》Call its " control pain and hinder lung qi, tonifying lung is empty, and cough-relieving is coughed, and disappear lung rash hemoptysis, restrains lung qi ".The main component of the bletilla striata is
Bletilla glucomannan, main component is a large amount of water miscible polysaccharide of glucomannan, is widely used in food processing, medicine and daily use chemicals and produces
Product field.Bletilla glucomannan has regulation body metabolism, the content of growth factor in regulation cell, can strengthen the work of blood platelet factor III
Property, significantly shorten the clotting time and factor forms the time, suppress plasmin enzymatic activity, have well to local hemorrhage
Therapeutic effect；Bletilla glucomannan also has antiulcer action simultaneously, and can treat gastric and duodenal ulcer.Because the bletilla striata has height to bore
Property, gluey film can be formed in stomach, so as to promote ulcer surface to heal；Secondly Bletilla glucomannan also has functions that antiphlogistic antibacterial, the bletilla striata
In beneficiating ingredient have bacteriostasis to staphylococcus and streptococcus, a diaphragm can be partially formed, can control and prevent stomach
Infect in portion；Bletilla glucomannan is also used as auxiliary material and is added in medicine again, extends the dead time of main ingredient, reduces the poison of medicine
Property.
The experiment of system is carried out to bletilla polysaccharide intervention therapy in liver cancer in recent years, and has been confirmed in clinical application research white
Splendid achnatherum polysaccharide has functions that to block liver cancer feeding artery and its offshoot circulation, can effectively suppress the growth and recurrence of liver cancer.The bletilla striata
Polysaccharide is a kind of safe and effective cancer therapy drug and hepatic artery embolism agent, and when applying to zoopery treatment liver cancer, its curative effect is excellent
In immune response modifier, anti-angiogenic medicaments and traditional simple TACE treatments.The research such as money fine horse finds, by vain
Splendid achnatherum polysaccharide can substantially suppress the growth of hepatocellular carcinoma with reference to chemotherapeutic and ligation of hepatic artery as hepatic artery embolism agent, in vain
The intervention therapy in liver cancer of splendid achnatherum polysaccharide has its superiority and wide development prospect.Additionally, is also shown that in correlative study burst in treatment
The aspect, bletilla polysaccharide such as hemostasis, upper gastrointestinal massive hemorrhage and promotion wound skin healing in ulcer type colitis, operation
There is wide application space.Used as a kind of natural macromolecular material, bletilla polysaccharide is extremely rising biological dressing material
Material, with good biocompatibility and biodegradability.Peng Rui researchs find, with gelatin and Bletilla glucomannan as timbering material,
Chinese medical extract is compound to wherein, sponge-like porous material is prepared by freeze-drying, space is evenly distributed, be mutually connected
It is logical, for cultivating l cell, cell preferably tactophily can be made.
Due to the function progressively transparence of the development bletilla striata of science and technology, the bletilla striata market demand also increasingly expands, in recent years China
The wild bletilla striata of most area is excessively excavated, and causes its wild natural resources drastically to reduce, endangered, is arranged by country
Attach most importance to one of Wild Medicinal of point protection.And first the market supply method for increasing the bletilla striata is the growing surface for increasing the bletilla striata
Product, increases Plant Tissue Breeding dynamics, is second the utilization ratio for increasing bletilla striata medicinal efficacy composition.The present invention is based on the latter
The series of studies of development.The extraction process of the current bletilla striata is mostly extensive water extraction and alcohol precipitation method, and extraction efficiency is not high, and extract is pure
Spend not high, range of application relative narrower.In order to increase bletilla striata bio-resource exploitation, carry out effective effect constituents extraction of the bletilla striata etc.
Deep processing project, increases medicine extraction efficiency and utilization rate, increases the secondary value added of medicine resource, becomes highly significant.
The content of the invention
The invention provides a kind of Bletilla glucomannan extracting method, mainly include immersion-section-hot water return extraction-concentration-enzyme
Solution-decolourize-centrifugation -ol is heavy-and macroporous absorption-ion exchange the processing step such as adsorbs-dehydrates, through substantial amounts of parallel to having a competition
Test and orthogonal test, the final optimum extraction parameter for determining Bletilla glucomannan, technique is specifically comprised the steps of：
（1）Take the bletilla striata to be soaked with 40-55 DEG C of warm water, water material mass ratio is 4-7:1, soak time 3-5 hours, soak it
After cut into slices, it is standby；
（2）According to mass ratio 1:The ratio of 12-20, adds purified water in bletilla striata piece, first uses 180W-250W ultrasonications
15-25min, then heating and refluxing extraction is carried out, reflux extracting time 30-60min, 80-90 DEG C of refluxing extraction temperature, refluxing extraction
Slag liquid is separated after end, slag charge repeats refluxing extraction 2-3 times；
（3）Merge reflux extracting liquid, be concentrated into proportion for 1.03-1.10 stops；
（4）When solution to be concentrated is cooled to 40-55 DEG C, the enzyme preparation of concentrate quality 0.8-2.0% is added to be hydrolyzed, during hydrolysis
Between 40-60min, being subsequently adding the activated carbon of concentrate quality 0.3-0.5% carries out adsorption bleaching reagent removal taste, process time 20-
Supernatant is collected in 35min, last centrifugation；
（5）By volume 1:The ratio of 5-8, adds absolute ethyl alcohol in supernatant, and after alcohol precipitation 18-26h, precipitation is collected in centrifugation,
Precipitation uses absolute ethanol washing 2-3 times；
（6）Precipitation after washing adds distillation water dissolves, and solution sequentially passes through macroporous absorbent resin and ion exchange resin treatment,
Filtrate is collected after crossing post；
（7）Filtrate concentrate drying, obtains final product Bletilla glucomannan.
The slice thickness is 1.5-3mm.
The enzyme preparation be it is a kind of in trypsase, papain, neutral proteinase, carboxypeptidase, aminopeptidase or
Arbitrarily than several.
The macroporous absorbent resin is DB-8, DB-301, HPD-100 or NKA-9.
The ion exchange resin is DEAE-01, DEAE-52, DEAE-cellulose（DEAE-cellulose）
Or CL-6B.
The separation and Extraction purifying process for refining of Bletilla glucomannan of the present invention, interlocks, it is intended to improve the extraction of bletilla striata Chinese medicine step by step
The purity of rate and extract；The main composition of the bletilla striata is Bletilla glucomannan, and Bletilla glucomannan is a kind of glucomannan, water-soluble pole
By force, so being carried using hot water return method master, in order to lift effective rate of recovery of extraction, substantial amounts of parallel test and orthogonal has been carried out
The optimal parameters such as analysis of experiments, the requirement of material when finally drawing extraction, Extracting temperature, extraction time, extraction time.To what is done
Cut into slices after bletilla striata immersion again ultrasonic, destroy plant cell, be conducive to the dissolution of effective medicinal components；Containing substantial amounts of in extract
The impurity such as starch, protein, using enzymatic treatment, enzyme hydrolysis removes protein；Processed with activated carbon and solve bletilla striata coarse extraction
Thing has color and has the inferior position of strong taste of traditional Chinese medicine；Finally by macroporous absorbent resin and ion exchange resin, further remove
A small amount of protein and mineral ion, final products are odorless, tasteless, and the powder insoluble in ethanol, extract purity is higher, can
To be largely used in food finishing, medicine and cosmetic field, the inventive method reduces impurity, it is ensured that product efficacy it is steady
Qualitative, its inventive method is simple, easy to operate, is suitable to industrialized production and marketing application.
Specific embodiment
The present invention is described in further detail below by embodiment, but the scope of the present invention is not limited in described
Hold.
Embodiment 1：
（1）Take extra dry white wine splendid achnatherum to be soaked with 40 DEG C of warm waters, water material mass ratio is 4:1, immersion is cut into slices after 5 hours, is cut into slices
Thickness is 2mm, standby；
（2）It is 1 according to mass ratio:12 ratio, adds purified water in bletilla striata piece, first with 200w ultrasonication 22min, then
Heating and refluxing extraction is carried out, reflux extracting time 50min, 85 DEG C of refluxing extraction temperature, backflow separates slag liquid, slag charge weight after terminating
Multiple refluxing extraction 3 times；
（3）Merge reflux extracting liquid, be concentrated into proportion for 1.03 stop；
（4）When solution to be concentrated temperature is cooled to 45 DEG C, concentrate quality 1.0% enzyme preparation (trypsase+carboxypeptidase, quality are added
Than 1:1) it is hydrolyzed, hydrolysis time 50min, the activated carbon for being subsequently adding concentrate quality 0.3% carries out adsorption bleaching reagent removal
Supernatant is collected in taste, process time 25min, last centrifugation；
（5）By volume 1:6 ratio, adds absolute ethyl alcohol in supernatant, and precipitation is collected in alcohol precipitation 18h, centrifugation, and precipitation is used
Absolute ethanol washing 3 times；
（6）Precipitation after washing adds distilled water to be dissolved, and sequentially passes through macroporous absorbent resin（NKA-9）And amberlite
Fat（CL-6B）, filtrate is collected after crossing post；
（7）Filtrate concentrate drying, obtains final product Bletilla glucomannan.
Bletilla glucomannan to extracting uses phenolsulfuric acid total reducing sugar determination method and 3,5- dinitrosalicylic acid colorimetric reducing sugar tests
Method carries out determination of polysaccharide, and it is 23.5% to measure the recovery rate of Bletilla glucomannan, and the purity of Bletilla glucomannan reaches 95.21%；By Bletilla glucomannan
Being made into 1% aqueous solution carries out discriminating identification, and bletilla polysaccharide sol solution can directly carry out Feling reactions, and precipitation is not produced, but
Positive reaction can occur after hydrochloric acid or sulfuric acid treatment；Illustrate that the bletilla polysaccharide purity extracted is higher, impurity content is less, can
To apply in terms of food additives and medical material.
Embodiment 2
（1）Take extra dry white wine splendid achnatherum to be soaked with 50 DEG C of warm waters, water material mass ratio is 6:1, immersion is cut into slices after 4 hours, is cut into slices
Thickness is 3mm, standby；
（2）It is 1 according to mass ratio:18 ratio, adds purified water in bletilla striata piece, first with 180W ultrasonication 18min, then
Heating and refluxing extraction is carried out, reflux extracting time 45min, 88 DEG C of refluxing extraction temperature, backflow separates slag liquid, slag charge weight after terminating
Multiple refluxing extraction 3 times；
（3）Merge reflux extracting liquid, be concentrated into proportion for 1.05 stop；
（4）When solution to be concentrated temperature is cooled to 45 DEG C, the enzyme preparation (trypsase) of concentrate quality 1.2% is added to be hydrolyzed, water
Solution time 55min, adding the activated carbon of concentrate quality 0.4% carries out adsorption bleaching reagent removal taste, process time 20min, then
It is centrifuged and collects supernatant；
（5）By volume 1:5 ratio, adds absolute ethyl alcohol in supernatant, and precipitation is collected in alcohol precipitation 24h, centrifugation, and precipitation is used
Absolute ethanol washing 2 times；
（6）Precipitation after washing adds distilled water to be dissolved, and sequentially passes through macroporous absorbent resin（HPD-100）And ion exchange
Resin（DEAE-cellulose）, filtrate is collected after crossing post；
（7）Filtrate concentrate drying, obtains final product Bletilla glucomannan.
Bletilla glucomannan to extracting uses phenolsulfuric acid total reducing sugar determination method and 3,5- dinitrosalicylic acid colorimetric reducing sugar tests
Method carries out determination of polysaccharide, and it is 24.7% to measure the recovery rate of Bletilla glucomannan, and the purity of Bletilla glucomannan reaches 93.3%.Bletilla glue is made into
1% aqueous solution carries out discriminating identification, and refined bletilla polysaccharide sol solution directly carries out Feling reactions, and precipitation, but warp are not produced
Positive reaction can occur after hydrochloric acid or sulfuric acid treatment.
Embodiment 3
（1）Take extra dry white wine splendid achnatherum to be soaked with 46 DEG C of warm waters, water material mass ratio is 4.5:1, immersion is cut into slices after 4.5 hours,
Slice thickness is 1.5mm, standby；
（2）It is 1 according to mass ratio:16 ratio, adds purified water in bletilla striata piece, first with 250W ultrasonication 15min, then
Heating and refluxing extraction is carried out, reflux extracting time 60min, 85 DEG C of refluxing extraction temperature, backflow separates slag liquid, slag charge weight after terminating
Multiple refluxing extraction 3 times；
（3）Merge reflux extracting liquid, be concentrated into proportion for 1.09 stop；
（4）When solution to be concentrated temperature is cooled to 55 DEG C, concentrate quality 1.5% enzyme preparation (papain+aminopeptidase, matter are added
Amount compares 2:1) it is hydrolyzed, hydrolysis time 55min, the activated carbon for being subsequently adding concentrate quality 0.4% carries out adsorption bleaching reagent removal
Then taste, process time 30min is centrifuged and collects supernatant；
（5）By volume 1:7 ratio, adds absolute ethyl alcohol in supernatant, and precipitation is collected in alcohol precipitation 22h, centrifugation, and precipitation is used
Absolute ethanol washing 3 times；
（6）Precipitation after washing adds distilled water to be dissolved, and sequentially passes through macroporous absorbent resin（DB-8）And amberlite
Fat（DEAE-01）, filtrate is collected after crossing post；
（7）Filtrate concentrate drying, obtains final product Bletilla glucomannan.
Bletilla glucomannan to extracting uses phenolsulfuric acid total reducing sugar determination method and 3,5- dinitrosalicylic acid colorimetric reducing sugar tests
Method carries out determination of polysaccharide, and it is 23.0% to measure the recovery rate of Bletilla glucomannan, and the purity of Bletilla glucomannan reaches 94.05%.Bletilla glue is matched somebody with somebody
The aqueous solution into 1% carries out discriminating identification, and refined bletilla polysaccharide sol solution directly carries out Feling reactions, and precipitation is not produced, but
Positive reaction can occur after hydrochloric acid or sulfuric acid treatment.
Claims (5)
1. a kind of Bletilla glucomannan extracting method, it is characterised in that comprise the steps of：
（1）Take the bletilla striata to be soaked with 40-55 DEG C of warm water, water material mass ratio is 4-7:1, immersion is cut into slices after 3-5 hours, standby
With；
（2）According to mass ratio 1:The ratio of 12-20, adds purified water in bletilla striata piece, first uses 180W-250W ultrasonications
15-25min, then heating and refluxing extraction is carried out, reflux extracting time 30-60min, 80-90 DEG C of refluxing extraction temperature, refluxing extraction
Slag liquid is separated after end, slag charge repeats refluxing extraction 2-3 times；
（3）Merge reflux extracting liquid, be concentrated into proportion for 1.03-1.10 stops；
（4）When solution to be concentrated is cooled to 40-55 DEG C, the enzyme preparation of concentrate quality 0.8-2.0% is added to be hydrolyzed, during hydrolysis
Between 40-60min, being subsequently adding the activated carbon of concentrate quality 0.3-0.5% carries out adsorption bleaching reagent removal taste, process time 20-
Supernatant is collected in 35min, last centrifugation；
（5）By volume 1:The ratio of 5-8, adds absolute ethyl alcohol in supernatant, and after alcohol precipitation 18-26h, precipitation is collected in centrifugation,
Precipitation uses absolute ethanol washing 2-3 times；
（6）Precipitation after washing adds distillation water dissolves, and solution sequentially passes through macroporous absorbent resin and ion exchange resin treatment,
Filtrate is collected after crossing post；
（7）Filtrate concentrate drying, obtains final product Bletilla glucomannan.
2. Bletilla glucomannan extracting method according to claim 1, it is characterised in that：Slice thickness is 1.5-3mm.
3. Bletilla glucomannan extracting method according to claim 1, it is characterised in that：Enzyme preparation is trypsase, Papain
In enzyme, neutral proteinase, carboxypeptidase, aminopeptidase one or more.
4. Bletilla glucomannan extracting method according to claim 1, it is characterised in that：Macroporous absorbent resin be DB-8, DB-301,
HPD-100 or NKA-9.
5. Bletilla glucomannan extracting method according to claim 1, it is characterised in that：Ion exchange resin be DEAE-01,
DEAE-52, DEAE-cellulose or CL-6B.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710155280.7A CN106893000A (en) | 2017-03-16 | 2017-03-16 | A kind of Bletilla glucomannan extracting method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710155280.7A CN106893000A (en) | 2017-03-16 | 2017-03-16 | A kind of Bletilla glucomannan extracting method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106893000A true CN106893000A (en) | 2017-06-27 |
Family
ID=59192309
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710155280.7A Pending CN106893000A (en) | 2017-03-16 | 2017-03-16 | A kind of Bletilla glucomannan extracting method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106893000A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107362141A (en) * | 2017-08-16 | 2017-11-21 | 深圳大佛药业股份有限公司 | A kind of Anefrin Nasal Spray and preparation method thereof |
| CN107714299A (en) * | 2017-12-04 | 2018-02-23 | 广东泰宝医疗科技股份有限公司 | A kind of medical dressing is dispelled pain patch |
| CN109970879A (en) * | 2019-03-21 | 2019-07-05 | 浙江中医药大学 | A kind of bletilla polysaccharide extract and preparation method thereof |
| CN112618470A (en) * | 2020-12-03 | 2021-04-09 | 重庆慧雍生物科技有限公司 | Traditional Chinese medicine formula skin care product containing Mn SOD and preparation method thereof |
| CN113304088A (en) * | 2021-05-26 | 2021-08-27 | 上海辉文生物技术股份有限公司 | Preparation method of bletilla striata extract with repairing and whitening effects and bletilla striata extract |
| CN115970047A (en) * | 2023-01-28 | 2023-04-18 | 贵州省农作物品种资源研究所 | Preparation method of bletilla striata gel freeze-dried powder, and dry powder solid dressing and liquid dressing containing bletilla striata freeze-dried powder |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101195839A (en) * | 2007-12-28 | 2008-06-11 | 中华全国供销合作总社南京野生植物综合利用研究院 | Fine purification technique for bletilla striata polyoses glue |
| CN101429254A (en) * | 2008-12-05 | 2009-05-13 | 云南植物药业有限公司 | Bletilla striata polysaccharide, preparation method and new uses thereof |
| CN101899174A (en) * | 2010-08-11 | 2010-12-01 | 珈侬生化科技(中国)有限公司 | Bletilla polysaccharide compound serving as cosmetic raw material and preparation method thereof |
| CN102453106A (en) * | 2010-10-20 | 2012-05-16 | 张安卉 | Preparation method of bletilla striata polysaccharide |
| CN103044560A (en) * | 2011-10-11 | 2013-04-17 | 欧阳靖云 | Preparation method of bletilla striata polysaccharide |
| CN105418783A (en) * | 2015-11-30 | 2016-03-23 | 深圳职业技术学院 | Bletilla striata polysaccharide extraction and purification method |
-
2017
- 2017-03-16 CN CN201710155280.7A patent/CN106893000A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101195839A (en) * | 2007-12-28 | 2008-06-11 | 中华全国供销合作总社南京野生植物综合利用研究院 | Fine purification technique for bletilla striata polyoses glue |
| CN101429254A (en) * | 2008-12-05 | 2009-05-13 | 云南植物药业有限公司 | Bletilla striata polysaccharide, preparation method and new uses thereof |
| CN101899174A (en) * | 2010-08-11 | 2010-12-01 | 珈侬生化科技(中国)有限公司 | Bletilla polysaccharide compound serving as cosmetic raw material and preparation method thereof |
| CN102453106A (en) * | 2010-10-20 | 2012-05-16 | 张安卉 | Preparation method of bletilla striata polysaccharide |
| CN103044560A (en) * | 2011-10-11 | 2013-04-17 | 欧阳靖云 | Preparation method of bletilla striata polysaccharide |
| CN105418783A (en) * | 2015-11-30 | 2016-03-23 | 深圳职业技术学院 | Bletilla striata polysaccharide extraction and purification method |
Non-Patent Citations (3)
| Title |
|---|
| 张卫明 等: "《中国植物胶资源开发研究与利用》", 31 December 2008, 东南大学出版社 * |
| 李新华 等: "《粮油加工学 第3版》", 31 October 2016, 中国农业大学出版社 * |
| 郑玉林 等: "《中学化学实验手册》", 30 November 1990, 安徽教育出版社 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107362141A (en) * | 2017-08-16 | 2017-11-21 | 深圳大佛药业股份有限公司 | A kind of Anefrin Nasal Spray and preparation method thereof |
| CN107362141B (en) * | 2017-08-16 | 2018-06-05 | 深圳大佛药业股份有限公司 | A kind of Anefrin Nasal Spray and preparation method thereof |
| CN107714299A (en) * | 2017-12-04 | 2018-02-23 | 广东泰宝医疗科技股份有限公司 | A kind of medical dressing is dispelled pain patch |
| CN109970879A (en) * | 2019-03-21 | 2019-07-05 | 浙江中医药大学 | A kind of bletilla polysaccharide extract and preparation method thereof |
| CN109970879B (en) * | 2019-03-21 | 2022-03-08 | 浙江中医药大学 | Bletilla striata polysaccharide extract and preparation method thereof |
| CN112618470A (en) * | 2020-12-03 | 2021-04-09 | 重庆慧雍生物科技有限公司 | Traditional Chinese medicine formula skin care product containing Mn SOD and preparation method thereof |
| CN113304088A (en) * | 2021-05-26 | 2021-08-27 | 上海辉文生物技术股份有限公司 | Preparation method of bletilla striata extract with repairing and whitening effects and bletilla striata extract |
| CN115970047A (en) * | 2023-01-28 | 2023-04-18 | 贵州省农作物品种资源研究所 | Preparation method of bletilla striata gel freeze-dried powder, and dry powder solid dressing and liquid dressing containing bletilla striata freeze-dried powder |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106893000A (en) | A kind of Bletilla glucomannan extracting method | |
| CN103751058B (en) | Peony skin care product and preparation method thereof | |
| CN104829743B (en) | A kind of preparation method and its usage of Polysaccharides from Leaves of Moringa oleifera | |
| CN105504079A (en) | Process for producing astragalus polysaccharide by using ultrasonic technology | |
| CN106490608A (en) | A kind of Herba Dendrobii enhancing immunity oral liquid, electuary, capsule, tablet | |
| CN101974095A (en) | Method for extracting and separating Chinese narcissus polysaccharides | |
| CN106832021A (en) | A kind of preparation method of Chinese cassia tree slag Thick many candies | |
| CN104940280A (en) | Method for extracting total flavones from radix puerariae employing enzyme preparation | |
| CN105815703A (en) | Method for processing trichosanthes peel, cortex albiziae and hawthorn fruit healthcare powder | |
| CN105497109A (en) | Process for producing milkvetch root total flavones through microbial fermentation technology | |
| CN106892994A (en) | A kind of preparation method of blueberry residue Thick many candies | |
| CN106344684A (en) | Multistep extraction method of rose polyphenols | |
| CN105399852A (en) | Technology for producing radix astragali polysaccharide by utilization of alkaline process extraction technology | |
| CN106892989A (en) | A kind of blackberry, blueberry preparation method | |
| CN106880692A (en) | A kind of extracting method of capsicum leaf polyphenol | |
| CN106167531B (en) | A kind of method of enzyme process assisted extraction longan polysaccharide | |
| CN103445160A (en) | Method for preparing ginseng pulp through enzymolysis of pectinase and ginseng pulp | |
| CN105410947B (en) | A kind of broccoli dietary fiber preparation method containing glucorphanin | |
| CN114042092A (en) | Cordycepin mixture and effective separation method and application thereof | |
| CN107582582A (en) | A kind of technique of extraction purification Moringa flavones | |
| CN103509773A (en) | Extraction technology of bromelain | |
| CN103191166B (en) | Improve the method for Saus-surea obvallata (DC.) Edgew yield and the application of extract and this extract | |
| CN106892993A (en) | A kind of preparation method of honey raisin tree slag Thick many candies | |
| CN105440148A (en) | Process for producing astragalus polysaccharide by using water extraction technology | |
| CN102603450A (en) | Continuous extraction method of amino acid and pectin in watermelon peel |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170627 |