CN103509773A

CN103509773A - Extraction technology of bromelain

Info

Publication number: CN103509773A
Application number: CN201210206445.6A
Authority: CN
Inventors: 冯上水; 郑凑珍
Original assignee: Jiangxi Kang Jia Lyophylized Food Co Ltd
Current assignee: Jiangxi Kang Jia Lyophylized Food Co Ltd
Priority date: 2012-06-21
Filing date: 2012-06-21
Publication date: 2014-01-15

Abstract

Relating to the technical field of bromelain extraction, the invention provides an extraction technology of bromelain. The technology includes the processes of: 1. introducing the squeezed juice of pineapple waste into an adsorption column to conduct adsorption; 2. when a kaolin collection volume reaches 50 billion enzyme activity units, stopping column loading; 3. adding hydrochloric acid into a high valence filtrate (1:3), adjusting the PH to 4.8-5.2 and performing acidification; 4. centrifuging a salt out substance; 5. putting a crude enzyme into a dissolving tank; 6. conducting stirring; 7. performing discharge; 8. sieving a wet product by a 20-mesh sieve, carrying out pressure reduced drying for 4-8h under normal temperature and a vacuum degree ranging from -0.08 to 0.09MPa, and controlling the moisture below 0.2% to obtain a dry product; and 9. sieving the dry product through a 60-mesh sieve, then performing discharge, and conducting packaging in terms of 5kg per barrel. The technology provided in the invention greatly enhances the enzyme activity and prolongs the activity preservation time, makes the enzyme activity preservation time prolonged from 6 months to 18 months or more at room temperature without change.

Description

Bromeline extraction process

Technical field

The present invention relates to bromeline extractive technique field, be specifically related to bromeline manufacture craft.

Background technology

Bromeline is a kind of natural nontoxic, protein of having no side effect, as a kind of thin Ji Shui, generates peptide or amino acid forms the protein hydrolyzate with peculiar flavour.Be mainly used in food and medicine, especially in food, be widely used, hydrolyzable animal proteinum, hydrolysis sheep placental extract and soybean etc., make the proteolysis of food become small-molecular peptides or amino acid macro-molecular protein, be hydrolyzed to the precision work of the small molecules amino acid and protein meat product of easy absorption, can be made into corresponding biscuit pine agent, tenderizer etc., be applied to widely tenderization, biscuit is crisp, drinks clarification, flour improvement, soybean is decomposed, senior oral liquid, healthcare product etc., can increase aminoacids content in food, improvement flavour of food products, reduce costs, and can greatly improve food value, the beer beverage finings that Bing makes as non-biological stabilizer, wine fermentation agent etc. is applied to drinks l brewery industry, soy sauce brewing industry, drink production industry etc.Innovation point, be that technique preparation has had new improvement, catalysis blender has had new breakthrough, and freeze-dry process needs further to be improved, changed the level of activity that can not reach from theoretical analysis in history, high reactivity is difficult to do and break through technically.

Summary of the invention

The object of this invention is to provide bromeline extraction process, the active shelf time of the activity of the raising enzyme that it is larger and prolongation, more than the public relations of technology is brought up to 1,500,000 U/g enzymic activity from 500,000 U/g, and guaranteeing is not affecting under active prerequisite, control moisture in 5% left and right, enzymic activity is protected at normal temperatures within 6 months, bring up to 18 months above and constant; Growth that can inhibition tumor cell, prevention and cure of cardiovascular disease, the secretion of inhibition toxin; Can be used for decrustation after burn, anti-inflammatory etc., and strengthen drug absorption power.

In order to solve the existing problem of background technology, the present invention is by the following technical solutions: its technical process: 1, the squeezeding juice of pineapple tankage is passed in adsorption column and adsorbed, upper prop coutroi velocity is 800 ~ 1000L/ h; Waste liquid seals in lower prop when 200u/m1 is above, enters sewage works when 200u/ml is following; 2, after reaching 500 hundred million enzyme activity units, kaolin set amount stops upper prop, with in 15%NaOH and kaolin to effluent liquid PH7.0 ± 0.2, drain after resin, pass into wash-out after 7 ~ 9%NaC1 solution soaking 30min, elution flow rate is controlled at 800 ~ 1000L/h, collects elutriant and is high price filtrate; 3, toward high price, add in filtrate hydrochloric acid (1:3) to regulate PH5.0 ± 0.2 to carry out acidifying, then add high price filtrate 25%(W/V) vitriol saltout, until completely dissolved, 4 ℃ of standing 12h; 4, the thing of saltouing is centrifugal, and the thick enzyme of bromeline that is afterwards for 2 ~ 3 times with 4 ℃ of purified water drip washing; 5, thick enzyme is dropped in dissolving vessel, add the purified water of 10 times, with 20%NaOH, adjust PH7.0 ~ 7.5 while stirring, make solution clarification, filter, scavenging solution is pumped into treatment tank; 6, under agitation, with hydrochloric acid (1:3), regulate PH4.0 ± 0.2, enzyme was separated out in standing 1 hour; 7, blowing, by whizzer rejection filter after 20 ~ 30 minutes, obtains bromeline wet product with after cold purified water drip washing 2 ~ 3 times; 8, wet product is crossed 20 mesh sieves; Normal temperature drying under reduced pressure 4 ~ 8h under vacuum tightness-0.08 ~ 0.09MPa, controls moisture and below 0.2%, obtains dry product; 9, dry product is crossed after 60 mesh sieves; Blowing, packs by 5kg/ bucket.

The present invention has following beneficial effect:

1, the growth of inhibition tumor cell.

2, to the control of cardiovascular disorder, be useful, heart attack and apoplexy that energy anticoagulant causes, allevating angina pectoris symptom, relaxes artery and shrinks, the decomposition of accelerating fibers proteinogen.

3, suppress toxin secretion; Experimental results show that Traumanase can reduce toxin and also can suppress the secretion effect of heat-labile toxin (LI), Toxins,exo-, cholera (CT) and heat-stable toxin (ST) in conjunction with activity.

4, for decrustation after burn; Traumanase can optionally remove Exfoliating Scrub, and new skin grafting dermepenthesis is carried out as early as possible.Experimentation on animals proves, Traumanase has no adverse effects to contiguous normal skin.The local effect of using microbiotic not affect Traumanase.

5, antiinflammation; Traumanase can effectively be treated inflammation and oedema (comprising thrombophlebitis, Skeletal muscle injury, hemotoncus, stomatitis, diabetics's ulcer and sport injury) in various tissues; Traumanase has the potentiality that activate inflammatory reaction.Data speculation according to statistics, the mixture that contains Traumanase (containing Traumanase, trypsinase, violaguercitrin) is aspect efficacy and saferry, the antiphlogistic drug that is more better than steroid, the curative effect that the antibiotic of Traumanase and low dosage and the mixed method treatment rheumatic arthritis of other enzyme are expected to obtain; Traumanase also can be treated diarrhoea.

6, promote drug absorption; By Traumanase and various microbiotic (as tsiklomitsin, amoxycilline Trihydrate bp etc.) coupling, can improve its curative effect.Correlative study shows, it can promote microbiotic in the transmission of infection site, thereby reduces antibiotic dosage.According to inferring, for cancer therapy drug, also there is similar effect.In addition, Traumanase can promote the absorption of nutritive substance.

7, the active shelf time of active and prolongation of larger raising enzyme, more than the public relations of technology is brought up to 1,500,000 U/g enzymic activity from 500,000 U/g, and guaranteeing is not affecting under active prerequisite, control moisture in 5% left and right, enzymic activity is protected at normal temperatures within 6 months, bring up to 18 months above and constant.

Embodiment:

This embodiment is by the following technical solutions: its technical process: 1, the squeezeding juice of pineapple tankage is passed in adsorption column and adsorbed, upper prop coutroi velocity is 800 ~ 1000L/ h; Waste liquid seals in lower prop when 200u/m1 is above, enters sewage works when 200u/ml is following; 2, after reaching 500 hundred million enzyme activity units, kaolin set amount stops upper prop, with in 15%NaOH and kaolin to effluent liquid PH7.0 ± 0.2, drain after resin, pass into wash-out after 7 ~ 9%NaC1 solution soaking 30min, elution flow rate is controlled at 800 ~ 1000L/h, collects elutriant and is high price filtrate; 3, toward high price, add in filtrate hydrochloric acid (1:3) to regulate PH5.0 ± 0.2 to carry out acidifying, then add high price filtrate 25%(W/V) vitriol saltout, until completely dissolved, 4 ℃ of standing 12h; 4, the thing of saltouing is centrifugal, and the thick enzyme of bromeline that is afterwards for 2 ~ 3 times with 4 ℃ of purified water drip washing; 5, thick enzyme is dropped in dissolving vessel, add the purified water of 10 times, with 20%NaOH, adjust PH7.0 ~ 7.5 while stirring, make solution clarification, filter, scavenging solution is pumped into treatment tank; 6, under agitation, with hydrochloric acid (1:3), regulate PH4.0 ± 0.2, enzyme was separated out in standing 1 hour; 7, blowing, by whizzer rejection filter after 20 ~ 30 minutes, obtains bromeline wet product with after cold purified water drip washing 2 ~ 3 times; 8, wet product is crossed 20 mesh sieves; Normal temperature drying under reduced pressure 4 ~ 8h under vacuum tightness-0.08 ~ 0.09MPa, controls moisture and below 0.2%, obtains dry product; 9, dry product is crossed after 60 mesh sieves; Blowing, packs by 5kg/ bucket.

The active shelf time of the activity of the raising enzyme that this embodiment is larger and prolongation, more than the public relations of technology is brought up to 1,500,000 U/g enzymic activity from 500,000 U/g, and guaranteeing is not affecting under active prerequisite, control moisture in 5% left and right, enzymic activity is protected at normal temperatures within 6 months, bring up to 18 months above and constant; Growth that can inhibition tumor cell, prevention and cure of cardiovascular disease, the secretion of inhibition toxin; Can be used for decrustation after burn, anti-inflammatory etc., and strengthen drug absorption power.

Claims

1. bromeline extraction process, is characterized in that its technical process is: 1, the squeezeding juice of pineapple tankage is passed in adsorption column and adsorbed, upper prop coutroi velocity is 800 ~ 1000L/ h; Waste liquid seals in lower prop when 200u/m1 is above, enters sewage works when 200u/ml is following; 2, after reaching 500 hundred million enzyme activity units, kaolin set amount stops upper prop, with in 15%NaOH and kaolin to effluent liquid PH7.0 ± 0.2, drain after resin, pass into wash-out after 7 ~ 9%NaC1 solution soaking 30min, elution flow rate is controlled at 800 ~ 1000L/h, collects elutriant and is high price filtrate; 3, toward high price, add in filtrate hydrochloric acid (1:3) to regulate PH5.0 ± 0.2 to carry out acidifying, then add high price filtrate 25%(W/V) vitriol saltout, until completely dissolved, 4 ℃ of standing 12h; 4, the thing of saltouing is centrifugal, and the thick enzyme of bromeline that is afterwards for 2 ~ 3 times with 4 ℃ of purified water drip washing; 5, thick enzyme is dropped in dissolving vessel, add the purified water of 10 times, with 20%NaOH, adjust PH7.0 ~ 7.5 while stirring, make solution clarification, filter, scavenging solution is pumped into treatment tank; 6, under agitation, with hydrochloric acid (1:3), regulate PH4.0 ± 0.2, enzyme was separated out in standing 1 hour; 7, blowing, by whizzer rejection filter after 20 ~ 30 minutes, obtains bromeline wet product with after cold purified water drip washing 2 ~ 3 times; 8, wet product is crossed 20 mesh sieves; Normal temperature drying under reduced pressure 4 ~ 8h under vacuum tightness-0.08 ~ 0.09MPa, controls moisture and below 0.2%, obtains dry product; 9, dry product is crossed after 60 mesh sieves; Blowing, packs by 5kg/ bucket.