CN110810688A - Pineapple beverage and preparation method thereof - Google Patents
Pineapple beverage and preparation method thereof Download PDFInfo
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- CN110810688A CN110810688A CN201911183685.7A CN201911183685A CN110810688A CN 110810688 A CN110810688 A CN 110810688A CN 201911183685 A CN201911183685 A CN 201911183685A CN 110810688 A CN110810688 A CN 110810688A
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- pineapple
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- bromelain
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- 238000001556 precipitation Methods 0.000 claims description 7
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- 229930003268 Vitamin C Natural products 0.000 description 1
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- 150000001413 amino acids Chemical class 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
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- 229910052742 iron Inorganic materials 0.000 description 1
- 230000008338 local blood flow Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000011664 nicotinic acid Substances 0.000 description 1
- 235000001968 nicotinic acid Nutrition 0.000 description 1
- 229960003512 nicotinic acid Drugs 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
- A23L2/382—Other non-alcoholic beverages fermented
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
- A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
- A23L33/22—Comminuted fibrous parts of plants, e.g. bagasse or pulp
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention provides a pineapple beverage and a preparation method thereof. The pineapple beverage prepared by the invention is prepared by utilizing a fermentation technology and adding bromelain and dietary fiber, combines the characteristics of fermentation, benefiting intestinal health, promoting intestinal peristalsis and promoting intestinal absorption by bromelain, is beneficial to nutrient absorption and intestinal health, simultaneously maximizes the comprehensive utilization of pineapples, and can keep the good activity of bromelain within 1 year by being packaged in a dark place.
Description
Technical Field
The invention relates to the technical field of foods, and particularly relates to a pineapple beverage and a preparation method thereof.
Background
Pineapple, also called pineapple, Chinese as pineapple, papaya, fructus pockmarki, verlap and so on, belongs to the pineapple family, pineapple, perennial herb. The pineapple is rich in nutrition, the content of vitamin C is far higher than that of the apple, the pineapple protease is rich, the protein digestion of a human body can be effectively promoted, fibrin and blood clots blocked in tissues can be dissolved, the local blood circulation is improved, and inflammation and edema are eliminated. According to scientific determination, the pineapple contains rich fructose, glucose, amino acid, organic acid, protein, fat, crude fiber, calcium, phosphorus, iron, carotene, multiple vitamins, nicotinic acid and other nutrient substances, and has high edible value and dietary therapy value.
The pineapple peel is the leftover of pineapple processing, the nutrient content of the pineapple peel is close to the basic component of the pulp, 50-60% of the pineapple peel is discarded by a processing factory in the processing process of the pineapple, huge waste of resources is caused, meanwhile, the processing area is also polluted by the environment, and the difficulty of pineapple peel garbage disposal is increased after garbage classification.
In view of the above, the present invention is particularly proposed.
Disclosure of Invention
According to the preparation method of the pineapple beverage provided by the invention, bromelain and soluble dietary fiber are added into the fermentation pulp, the pineapple beverage is obtained after blending, the pineapple beverage is prepared by utilizing a fermentation technology and adding bromelain and dietary fiber, the characteristics of benefiting intestinal health through fermentation and promoting intestinal peristalsis and the characteristics of promoting intestinal absorption through bromelain are combined, the nutrition absorption and the intestinal health are facilitated, and meanwhile, the comprehensive utilization of pineapples is maximized.
In order to achieve the above purpose of the present invention, the following technical solutions are adopted:
a preparation method of pineapple drink comprises the following steps:
(a) peeling the pineapples, respectively collecting pineapple pulp and pineapple peel residues, pulping the pineapple pulp, and filtering to obtain pineapple pulp;
(b) sterilizing, fermenting, homogenizing and inactivating the pineapple pulp obtained in the step (a) to obtain pineapple fermented pulp;
(c) juicing the pineapple peel residues collected in the step (a), filtering to obtain filtrate and fruit scraps respectively, adding benzoic acid into the filtrate, then adding kaolin for adsorption, and removing supernatant to obtain an adsorbate;
optionally, the addition amount of the benzoic acid is 0.025-0.05% of the mass of the filtrate;
optionally, the adding amount of the kaolin is 4-6% of the mass of the filtrate;
(d) adjusting the pH value of the adsorbate in the step (c) to 6.7-7.0, adding a sodium chloride solution, stirring, filtering, adjusting the pH value of the filtrate to 4.8-5.1, adding ammonium sulfate, salting out, standing for precipitation, and drying the precipitate to obtain bromelain;
optionally, adjusting the pH of the adsorbate to 6.7-7.0 with sodium hydroxide, preferably, the mass concentration of the sodium hydroxide is 16%;
optionally, the mass fraction of the sodium chloride solution is 7% -9%;
optionally, adjusting the pH of the filtrate to 4.8-5.1 with hydrochloric acid;
optionally, the drying is freeze drying;
optionally, the filtration is filter pressing;
(e) adding water and cellulase into the fruit scraps in the step (c) and the residue obtained after extracting the bromelain in the step (d), adjusting the pH value of the solution to be 4.8-5.0, centrifuging and collecting supernatant after reaction, and concentrating to obtain a pineapple soluble dietary fiber concentrated solution;
optionally, before adding water and cellulase, the method further comprises the steps of cleaning and filtering, preferably, the mesh number of a sieve used for filtering is 100-120 meshes;
optionally, the concentration is 8-10 times;
(f) diluting the pineapple fermentation pulp obtained in the step (b), mixing the pineapple fermentation pulp with the bromelain obtained in the step (d), the pineapple soluble dietary fiber concentrated solution obtained in the step (e), cysteine and EDTA, and sterilizing to obtain the pineapple beverage;
optionally, the adding amount of the bromelain is 40-80U/kg;
optionally, the addition amount of the pineapple soluble dietary fiber concentrated solution is 1-3 g/kg;
optionally, the addition amount of the cysteine is 0.004-0.006% of the mass sum of the diluted pineapple fermented pulp, the diluted bromelain and the diluted pineapple soluble dietary fiber concentrated solution;
optionally, the addition amount of the EDTA is 0.05-0.08% of the mass sum of the diluted pineapple fermented pulp, the diluted bromelain and the diluted pineapple soluble dietary fiber concentrated solution;
optionally, the sterilization is ultra-high pressure sterilization, the pressure of the ultra-high pressure sterilization is 400-.
Optionally, in step (a), the filtration is performed with a 100-120 mesh sieve.
Optionally, in step (b), the temperature of sterilization is 90-110 ℃.
Optionally, in the step (b), the microbial inoculum used for fermentation is selected from lactobacillus plantarum and/or lactobacillus casei;
preferably, the inoculation amount of the microbial inoculum is 0.005-0.01% of the mass of the pineapple pulp;
preferably, the fermentation temperature is 32-38 ℃, and the fermentation time is 35-42 h.
Optionally, in step (b), the pressure of the homogenization treatment is 30-60 Mpa.
Optionally, in step (b), the inactivation temperature is 90-110 ℃, and the inactivation time is 15-20 min.
Optionally, in the step (e), in the step of adding water and cellulase, the addition amount of water is 10-15 times of the sum of the mass of the fruit crumbs and the mass of the residue;
preferably, the concentration of the cellulase is 3000-5000U/kg;
preferably, the reaction temperature is 40-45 ℃, and the reaction time is 2-2.5 h.
Optionally, in the step (d), the addition amount of the sodium chloride is 4% -6% of the mass of the adsorbent.
Optionally, in the step (d), the addition amount of the ammonium sulfate is 2-4% of the mass of the filtrate.
The pineapple beverage is prepared by the preparation method of the pineapple beverage.
Compared with the prior art, the invention has the beneficial effects that:
(1) according to the preparation method of the pineapple beverage provided by the invention, bromelain and soluble dietary fiber are added into the fermentation pulp, the pineapple beverage is obtained after blending, the pineapple beverage is prepared by utilizing a fermentation technology and adding bromelain and dietary fiber, the characteristics of benefiting intestinal health through fermentation and promoting intestinal peristalsis and the characteristics of promoting intestinal absorption through bromelain are combined, the nutrition absorption and the intestinal health are facilitated, and meanwhile, the comprehensive utilization of pineapples is maximized.
(2) The pineapple beverage provided by the invention can keep good activity of bromelain within 1 year by being packaged in a dark place.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the following detailed description, but those skilled in the art will understand that the following described examples are some, not all, of the examples of the present invention, and are only used for illustrating the present invention, and should not be construed as limiting the scope of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.
The preparation method of the pineapple beverage provided by the invention comprises the following steps:
(a) peeling the pineapples, respectively collecting pineapple pulp and pineapple peel residues, pulping the pineapple pulp, and filtering to obtain pineapple pulp;
(b) sterilizing, fermenting, homogenizing and inactivating the pineapple pulp obtained in the step (a) to obtain pineapple fermented pulp;
(c) juicing the pineapple peel residues collected in the step (a), filtering to obtain filtrate and fruit scraps respectively, adding benzoic acid into the filtrate, then adding kaolin for adsorption, and removing supernatant to obtain an adsorbate;
in some preferred embodiments of the present invention, benzoic acid is used as a preservative to prevent the growth of microorganisms during the extraction process and affect the quality of the pineapple drink, and preferably, the amount of benzoic acid added is 0.025-0.05% of the mass of the filtrate;
in some preferred embodiments of the present invention, kaolin is used as an adsorbent, and the amount of kaolin added is 4% -6% of the mass of the filtrate;
(d) adjusting the pH value of the adsorbate in the step (c) to 6.7-7.0, adding a sodium chloride solution, stirring, filtering, adjusting the pH value of the filtrate to 4.8-5.1, adding ammonium sulfate, salting out, standing for precipitation, and drying the precipitate to obtain bromelain;
in some preferred embodiments of the present invention, the pH of the adsorbate is adjusted to 6.7-7.0 using sodium hydroxide, preferably, the mass concentration of the sodium hydroxide is 16%;
in some preferred embodiments of the invention, the sodium chloride solution has a mass fraction of 7% to 9%;
in some preferred embodiments of the present invention, the pH of the filtrate is adjusted to 4.8-5.1 with hydrochloric acid;
in some preferred embodiments of the invention, the drying is freeze-drying;
in some preferred embodiments of the present invention, the filtration is performed by pressure filtration;
(e) adding water and cellulase into the fruit scraps in the step (c) and the residue obtained after extracting the bromelain in the step (d), adjusting the pH value of the solution to be 4.8-5.0, centrifuging and collecting supernatant after reaction, and concentrating to obtain a pineapple soluble dietary fiber concentrated solution;
in some preferred embodiments of the present invention, before adding water and cellulase, the method further comprises the steps of cleaning and filtering, preferably, the mesh number of the sieve used for filtering is 100-120 meshes;
in some preferred embodiments of the invention, the concentration is from 8 to 10 times;
(f) diluting the pineapple fermentation pulp obtained in the step (b), mixing the pineapple fermentation pulp with the bromelain obtained in the step (d), the pineapple soluble dietary fiber concentrated solution obtained in the step (e), cysteine and EDTA, and sterilizing to obtain the pineapple beverage;
in some preferred embodiments of the present invention, the amount of bromelain added is 40-80U/kg;
in some preferred embodiments of the present invention, the amount of the pineapple soluble dietary fiber concentrate added is 1-3 g/kg;
in some preferred embodiments of the present invention, the amount of cysteine added is 0.004-0.006% of the mass sum of the diluted pineapple fermented pulp, the bromelain and the pineapple soluble dietary fiber concentrate;
in some preferred embodiments of the present invention, the addition amount of the EDTA is 0.05 to 0.08 percent of the mass sum of the diluted pineapple fermented pulp, the diluted bromelain and the diluted pineapple soluble dietary fiber concentrate;
in some preferred embodiments of the present invention, the sterilization is ultra-high pressure sterilization, the pressure of the ultra-high pressure sterilization is 400-600MPa, and the time of the sterilization is 15-20 min.
In some preferred embodiments of the present invention, in step (a), the filtration is performed using a 100-mesh and 120-mesh sieve.
In some preferred embodiments of the present invention, in step (b), the temperature of the sterilization is 90-110 ℃.
In some preferred embodiments of the present invention, in step (b), the microbial inoculum used for fermentation is selected from lactobacillus plantarum and/or lactobacillus casei;
in some preferred embodiments of the invention, the inoculation amount of the microbial inoculum is 0.005-0.01% of the mass of the pineapple pulp;
in some preferred embodiments of the present invention, the temperature of the fermentation is 32-38 ℃ and the time of the fermentation is 35-42 h.
In some preferred embodiments of the present invention, in the step (b), the pressure of the homogenization treatment is 30 to 60 Mpa.
In some preferred embodiments of the present invention, in step (b), the temperature of the inactivation is 90-110 ℃, and the time of the inactivation is 15-20 min.
In some preferred embodiments of the present invention, in the step (e), the step of adding water and cellulase, the amount of water added is 10 to 15 times the sum of the masses of the fruit pieces and the residue;
preferably, the concentration of the cellulase is 3000-5000U/kg;
preferably, the reaction temperature is 40-45 ℃, and the reaction time is 2-2.5 h.
In some preferred embodiments of the invention, in step (d), the sodium chloride is added in an amount of 4% to 6% of the mass of the adsorbent.
In some preferred embodiments of the present invention, in step (d), the ammonium sulfate is added in an amount of 2% to 4% by mass of the filtrate.
The pineapple beverage prepared by the preparation method of the pineapple beverage provided by the invention.
Embodiments of the present invention will be described in detail below with reference to examples, but it will be understood by those skilled in the art that the following examples are only illustrative of the present invention and should not be construed as limiting the scope of the present invention. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products commercially available.
Example 1
The preparation method of the pineapple beverage provided by the embodiment specifically comprises the following steps:
1. pineapple pretreatment: peeling pineapples, and respectively collecting pineapple pulp and pineapple peel residues for later use; pulping pineapple pulp, filtering with 100 mesh sieve, and removing crude fiber to obtain pineapple pulp.
2. Fermentation: sterilizing pineapple pulp at 90 deg.C for 20min, adding Lactobacillus plantarum, fermenting at 32 deg.C for 42h, homogenizing under 30Mpa after fermentation is finished, and inactivating at 90 deg.C for 20min to obtain pineapple fermented pulp;
3. preparing bromelain: squeezing leftovers such as pineapple peel residue, filtering to obtain juice, adding 0.025% benzoic acid into the filtrate, adding 5% kaolin for adsorption, adjusting the pH of the kaolin adsorbed substance to 7.0 with saturated sodium carbonate solution, adding 5% sodium chloride, stirring, press-filtering, adjusting the pH of the filtrate to 4.8 with hydrochloric acid, adding 2% ammonium sulfate, standing for precipitation, taking the precipitate, and freeze-drying to obtain bromelain;
4. preparing dietary fiber: taking residue and fruit scraps after extraction of bromelain, washing with water, sieving with a 100-mesh sieve to obtain clean pineapple residue, and mixing the residue and water according to a ratio of 1: 10, adding 3000U/kg of cellulase, extracting at 40 ℃ for 2h, centrifuging to collect supernatant, and vacuum concentrating by 10 times to obtain pineapple soluble dietary fiber concentrated solution.
5. Blending the pineapple beverage: diluting the pineapple fermentation slurry by 3 times, adjusting the pH value to 5.0, adding 80U/kg of bromelain and 1g/kg of soluble dietary fiber concentrated solution, adding 0.004% of cysteine and 0.05% of EDTA, uniformly mixing, and performing ultra-high pressure sterilization for 15min to obtain the pineapple beverage.
Example 2
The preparation method of the pineapple beverage provided by the embodiment specifically comprises the following steps:
1. pineapple pretreatment: peeling pineapples, and respectively collecting pineapple pulp and pineapple peel residues for later use; pulping pineapple pulp, filtering with 100 mesh sieve, and removing crude fiber to obtain pineapple pulp.
2. Fermentation: sterilizing pineapple pulp at 110 ℃, adding lactobacillus plantarum and lactobacillus casei in a ratio of 1:1, mixing, fermenting for 42h at 38 ℃, homogenizing under high pressure of 60MPa after fermentation is finished, and inactivating at 110 ℃ for 15min to obtain pineapple fermented pulp;
3. preparing bromelain: squeezing leftovers such as pineapple peel residue, collecting juice, filtering to remove fruit scraps, adding 0.05% benzoic acid into the filtrate, adding 5% kaolin for adsorption, adjusting the pH of the kaolin adsorbed substance to 7.0 with saturated sodium carbonate solution, adding 5% sodium chloride, stirring, filter-pressing, adjusting the pH of the filtrate to 5.1 with hydrochloric acid, adding 4% ammonium sulfate, standing for precipitation, collecting the precipitate, and freeze-drying to obtain bromelain;
4. preparing dietary fiber: taking residue and fruit scraps after extraction of bromelain, washing with water, sieving with a 100-mesh sieve to obtain clean pineapple residue, and mixing the residue and water according to a ratio of 1: 10, adding 5000U/kg of cellulase, extracting at 40 ℃ for 2.5h with pH of 5.0, centrifuging, collecting supernatant, and vacuum concentrating by 10 times to obtain pineapple soluble dietary fiber concentrated solution.
5. Blending the pineapple beverage: diluting the pineapple fermented pulp by 3 times, adjusting the pH value to 6.5, adding 40U/kg of bromelain and 3g/kg of soluble dietary fiber concentrated solution, adding 0.006% of cysteine and 0.08% of EDTA, mixing uniformly, and performing ultra-high pressure sterilization for 20min to obtain the pineapple beverage.
Example 3
The preparation method of the pineapple beverage provided by the embodiment specifically comprises the following steps:
1. pineapple pretreatment: peeling pineapples, and respectively collecting pineapple pulp and pineapple peel residues for later use; pulping pineapple pulp, filtering with 100 mesh sieve, and removing crude fiber to obtain pineapple pulp.
2. Fermentation: sterilizing pineapple pulp at 100 deg.C, adding Lactobacillus plantarum, fermenting at 36 deg.C for 36 hr, homogenizing under 50MPa, and inactivating at 100 deg.C for 20min to obtain pineapple fermented pulp;
3. preparing bromelain: squeezing leftovers such as pineapple peel residue, collecting juice, filtering to remove fruit scraps, adding 0.04% benzoic acid into the filtrate, adding 5% kaolin for adsorption, adjusting the pH of the kaolin adsorbed substance to 6.8 with saturated sodium carbonate solution, adding 5% sodium chloride, stirring, filter-pressing, adjusting the pH of the filtrate to 5.0 with hydrochloric acid, adding 3% ammonium sulfate, standing for precipitation, collecting the precipitate, and freeze-drying to obtain bromelain;
4. preparing dietary fiber: taking residue and fruit scraps after extraction of bromelain, washing with water, sieving with a 100-mesh sieve to obtain clean pineapple residue, and mixing the residue and water according to a ratio of 1: 10, adding 4000U/kg of cellulase, extracting for 2.5h at the temperature of 40 ℃ and the pH value of 5.0, centrifuging, collecting supernate, and concentrating by 10 times in vacuum to obtain the pineapple soluble dietary fiber concentrated solution.
5. Blending the pineapple beverage: diluting the pineapple fermentation slurry by 3 times, adjusting the pH value to 5.0, adding 60U/kg of bromelain and 2g/kg of soluble dietary fiber concentrated solution, adding 0.004% of cysteine and 0.05% of EDTA, mixing uniformly, and performing ultra-high pressure sterilization for 20min to obtain the pineapple beverage.
Example 4
The preparation method of the pineapple beverage provided by the embodiment specifically comprises the following steps:
1. pineapple pretreatment: peeling pineapples, and respectively collecting pineapple pulp and pineapple peel residues for later use; pulping pineapple pulp, filtering with 100 mesh sieve, and removing crude fiber to obtain pineapple pulp.
2. Fermentation: sterilizing pineapple pulp at 95 deg.C, adding Lactobacillus casei, fermenting at 35 deg.C for 36 hr, homogenizing under 40Mpa after fermentation, and inactivating at 100 deg.C for 20min to obtain pineapple fermented pulp;
3. preparing bromelain: squeezing leftovers such as pineapple peel residue, filtering to obtain juice, adding 0.025% benzoic acid into the filtrate, adding 5% kaolin for adsorption, adjusting the pH of the kaolin adsorbed substance to 7.0 with saturated sodium carbonate solution, adding 5% sodium chloride, stirring, press-filtering, adjusting the pH of the filtrate to 5.1 with hydrochloric acid, adding 4% ammonium sulfate, standing for precipitation, and freeze-drying the precipitate to obtain bromelain;
4. preparing dietary fiber: taking residue and fruit scraps after extraction of bromelain, washing with water, sieving with a 100-mesh sieve to obtain clean pineapple residue, and mixing the residue and water according to a ratio of 1: 10, adding 4000U/kg of cellulase, extracting for 2.5h at the temperature of 40 ℃ and the pH value of 5.0, centrifuging, collecting supernate, and concentrating by 10 times in vacuum to obtain the pineapple soluble dietary fiber concentrated solution.
5. Blending the pineapple beverage: diluting the pineapple fermented pulp by 3 times, adjusting the pH value to 6.5, adding 50U/kg of bromelain and 3g/kg of soluble dietary fiber concentrated solution, adding 0.006% of cysteine and 0.08% of EDTA, mixing uniformly, and performing ultra-high pressure sterilization for 20min to obtain the pineapple beverage.
Experimental example determination of changes in the protease Activity of pineapple drink with time
The enzymatic activities of bromelain in the pineapple beverages obtained in examples 1 to 4 and comparative example as a function of time are shown in Table 1.
The comparative example is a beverage prepared by adding bromelain into 3-fold diluted pineapple raw pulp (without fermentation) and adding 3g/kg of soluble dietary fiber concentrated solution.
TABLE 1 variation of Bromelain Activity with time (U/kg) (storage temperature 25 ℃ C.)
Product(s) | Initial value | 2 months old | 4 months old | 6 months old | 8 months old | For 10 months | 12 months old |
Example 1 | 80.6 | 78.5 | 75.8 | 73.4 | 71.8 | 68.1 | 66.5 |
Example 2 | 40.6 | 40.2 | 38.6 | 35.2 | 34.1 | 32.1 | 30.4 |
Example 3 | 60.3 | 59.1 | 57.9 | 56.4 | 55.7 | 54.8 | 53.0 |
Example 4 | 50.7 | 48.4 | 46.7 | 45.9 | 43.7 | 42.1 | 40.3 |
Comparative example | 80.2 | 60.4 | 51.8 | 46.1 | 32.4 | 21.6 | 10.7 |
The experimental result shows that compared with the comparative example, the activity of the bromelain of the pineapple beverage provided by the application is better maintained. The pineapple nutritional health-care food is prepared by utilizing a fermentation technology and adding bromelain and dietary fibers, combines the characteristics of fermentation, benefiting the intestinal health, promoting the intestinal peristalsis and promoting the intestinal absorption by the bromelain, is beneficial to nutrient absorption and intestinal health, and simultaneously maximizes the comprehensive utilization of pineapples.
By packaging in a dark place, the pineapple beverage provided by the invention can keep good activity of bromelain within 1 year.
While particular embodiments of the present invention have been illustrated and described, it will be appreciated that the above embodiments are merely illustrative of the technical solution of the present invention and are not restrictive; those of ordinary skill in the art will understand that: modifications may be made to the above-described embodiments, or equivalents may be substituted for some or all of the features thereof without departing from the spirit and scope of the present invention; the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention; it is therefore intended to cover in the appended claims all such alternatives and modifications that are within the scope of the invention.
Claims (10)
1. The preparation method of the pineapple beverage is characterized by comprising the following steps:
(a) peeling the pineapples, respectively collecting pineapple pulp and pineapple peel residues, pulping the pineapple pulp, and filtering to obtain pineapple pulp;
(b) sterilizing, fermenting, homogenizing and inactivating the pineapple pulp obtained in the step (a) to obtain pineapple fermented pulp;
(c) juicing the pineapple peel residues collected in the step (a), filtering to obtain filtrate and fruit scraps respectively, adding benzoic acid into the filtrate, then adding kaolin for adsorption, and removing supernatant to obtain an adsorbate;
optionally, the addition amount of the benzoic acid is 0.025-0.05% of the mass of the filtrate;
optionally, the adding amount of the kaolin is 4-6% of the mass of the filtrate;
(d) adjusting the pH value of the adsorbate in the step (c) to 6.7-7.0, adding a sodium chloride solution, stirring, filtering, adjusting the pH value of the filtrate to 4.8-5.1, adding ammonium sulfate, salting out, standing for precipitation, and drying the precipitate to obtain bromelain;
optionally, adjusting the pH of the adsorbate to 6.7-7.0 with sodium hydroxide, preferably, the mass concentration of the sodium hydroxide is 16%;
optionally, the mass fraction of the sodium chloride solution is 7% -9%;
optionally, adjusting the pH of the filtrate to 4.8-5.1 with hydrochloric acid;
optionally, the drying is freeze drying;
optionally, the filtration is filter pressing;
(e) adding water and cellulase into the fruit scraps in the step (c) and the residue obtained after extracting the bromelain in the step (d), adjusting the pH value of the solution to be 4.8-5.0, centrifuging and collecting supernatant after reaction, and concentrating to obtain a pineapple soluble dietary fiber concentrated solution;
optionally, before adding water and cellulase, the method further comprises the steps of cleaning and filtering, preferably, the mesh number of a sieve used for filtering is 100-120 meshes;
optionally, the concentration is 8-10 times;
(f) diluting the pineapple fermentation pulp obtained in the step (b), mixing the pineapple fermentation pulp with the bromelain obtained in the step (d), the pineapple soluble dietary fiber concentrated solution obtained in the step (e), cysteine and EDTA, and sterilizing to obtain the pineapple beverage;
optionally, the adding amount of the bromelain is 40-80U/kg;
optionally, the addition amount of the pineapple soluble dietary fiber concentrated solution is 1-3 g/kg;
optionally, the addition amount of the cysteine is 0.004-0.006% of the mass sum of the diluted pineapple fermented pulp, the diluted bromelain and the diluted pineapple soluble dietary fiber concentrated solution;
optionally, the addition amount of the EDTA is 0.05-0.08% of the mass sum of the diluted pineapple fermented pulp, the diluted bromelain and the diluted pineapple soluble dietary fiber concentrated solution;
optionally, the sterilization is ultra-high pressure sterilization, the pressure of the ultra-high pressure sterilization is 400-.
2. The method for preparing pineapple drink of claim 1, wherein in step (a), the filtration is performed with 100-mesh and 120-mesh sieve.
3. The method for preparing pineapple drink according to claim 1, wherein the temperature for sterilization is 90 to 110 ℃ in the step (b).
4. The method for preparing pineapple drink according to claim 1, wherein in the step (b), the microbial inoculum for fermentation is selected from Lactobacillus plantarum and/or Lactobacillus casei;
preferably, the inoculation amount of the microbial inoculum is 0.005-0.01% of the mass of the pineapple pulp;
preferably, the fermentation temperature is 32-38 ℃, and the fermentation time is 35-42 h.
5. The method for preparing pineapple drink of claim 1, wherein the pressure of the homogenization treatment in the step (b) is 30 to 60 Mpa.
6. The method for preparing pineapple drink of claim 1, wherein in the step (b), the inactivation temperature is 90-110 ℃, and the inactivation time is 15-20 min.
7. The method for preparing pineapple drink according to claim 1, wherein in the step (e), water and cellulase are added in an amount of 10 to 15 times the sum of the mass of the fruit powder and the residue;
preferably, the concentration of the cellulase is 3000-5000U/kg;
preferably, the reaction temperature is 40-45 ℃, and the reaction time is 2-2.5 h.
8. The method for preparing pineapple drink according to claim 1, wherein in the step (d), the sodium chloride is added in an amount of 4 to 6% by mass based on the amount of the adsorbent.
9. The method for preparing pineapple drink according to claim 1, wherein ammonium sulfate is added in an amount of 2 to 4% by mass based on the filtrate in step (d).
10. A pineapple beverage prepared by the method for preparing a pineapple beverage as claimed in any one of claims 1 to 9.
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