CN108084139A - A kind of onion anthocyanidin preparation method - Google Patents
A kind of onion anthocyanidin preparation method Download PDFInfo
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- CN108084139A CN108084139A CN201711347610.9A CN201711347610A CN108084139A CN 108084139 A CN108084139 A CN 108084139A CN 201711347610 A CN201711347610 A CN 201711347610A CN 108084139 A CN108084139 A CN 108084139A
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
- C07D311/58—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4
- C07D311/60—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2
- C07D311/62—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2 with oxygen atoms directly attached in position 3, e.g. anthocyanidins
Abstract
The present invention relates to anthocyanidin processing technique fields, more particularly to a kind of onion anthocyanidin preparation method, the application pre-processes onion using biological enzyme, probiotics, biological enzyme is mixed to prepare by pectase, cellulase and Pseudomonas cepacia lipase, can effectively broken wall be carried out for the cell membrane of onion, accelerate the precipitation of onion cells Dissolve things inside matter, the probiotics that the probiotics of the application obtains after screening and culturing medium screens has stronger antibacterial ability, onion cells can be effectively decomposed, promote the precipitation of anthocyanidin；The application is extracted using subcritical 1,1,1,2 tetrafluoroethane fluid, and extract is further purified in macroreticular resin, can the safe and effective anthocyanidin yield and recovery rate for improving onion.
Description
【Technical field】
The present invention relates to anthocyanidin processing technique field, more particularly to a kind of onion anthocyanidin preparation method.
【Background technology】
Plant source anthocyanidin is important natural function food additives, is widely used in beverage, candy, cake, drinks
And the industries such as food.
Onion (scientific name：Allium cepa), alias ball green onion, round onions, jade onion, onion, Dutch green onion, skin serratd edge etc., lily
Section, allium biennial herb plant contain anthocyanidin in onion, and anthocyanidin has important physiological active functions, and having can be with
Interior free yl is removed, there is anti-oxidant, anti-aging, improve eyesight, inhibit tumour etc.；Also there is eye health, heart rate
Health care reduces dangerous pathogenesis of cancer, enhancing blood vessel intensity, anti-inflammatory, the multiple efficacies such as swollen of dispelling；At present, the extraction of anthocyanidin is adopted more
With HFA 134a extraction, although HFA 134a extraction can dissolve out anthocyanidin, since plant is thin
The inhibition of cell wall, cell membrane, solvability is relatively low, moreover, directly being extracted using HFA 134a can make
The drawbacks of destroying Product Safety into the uses of a large amount of HFA 134as, it is therefore necessary to provide it is a kind of safely, effectively
And the anthocyanidin yield of onion and the preparation method of recovery rate can be improved.
【The content of the invention】
In view of the above, it is necessary to provide and a kind of safely, effectively and effectively improve anthocyanidin yield and the extraction of onion
The preparation method of rate.
In order to achieve the above objectives, the technical solution adopted in the present invention is：
A kind of onion anthocyanidin preparation method, described method includes following steps：
(1) it will be shredded after the cleaning of ripe onion, removal of impurities, be then 1 according to solid-liquid mass ratio:2-4 is mixed with water, is put
Enter and slurries are broken into beater, after filtering filtrate is taken to obtain Conditions of Onion Juice；
(2) digested, digested under 35-40 DEG C of temperature conditionss according to the addition enzyme solutions of 0.1g/ml-0.5g/ml
It is once centrifuged after 8h-12h and supernatant is taken to carry out probiotics processing with probiotic solution, secondary centrifuging is carried out again and takes supernatant
Obtain onion clear liquid；The centrifugal rotational speed of the centrifugation is 2000r/min-2500r/min；Centrifugation time is 15min-
20min；The additive amount of the probiotics is 0.2g/ml-0.6g/ml, and probiotics treatment temperature is 23-27 DEG C, and processing time is
18h-20h；The centrifugal rotational speed of the secondary centrifuging is 4000r/min-5000r/min；Centrifugation time is 20min-30min；
(3) the onion clear liquid that step (2) obtains is subjected to rotary evaporation and is concentrated to give density as 3.26g/cm3-4.15g/
cm3Paste, then paste is placed in extraction kettle, pressure be 4-5MPa when, with HFA 134a to cream
Shape object carries out static extracting 60-80min, then progressively discharges pressure, carries out dynamic extraction, and the dynamic extraction time is
50min-60min；The HFA 134a rate of discharge of dynamic extraction is 0.7-0.9L/min, dissolved with the 1 of product, 1,1,
2- tetrafluoroethane leaves the steel pipe for being tied with heating tape by one section after extraction kettle, is discharged after being depressurized by pressure reducing valve and obtains onion cyanine
Plain crude extract；
(4) HFA 134a being concentrated in vacuo in removal step (3) obtains onion anthocyanidin clear liquid, by anthocyanidin
Clear liquid is adsorbed with large pore resin absorption column, and during absorption, column flow rate is 0.5-1 times of column volume/hour on anthocyanidin clear liquid, on
Column temperature is 30-40 DEG C, is eluted after the completion of absorption with the ethanol solution that percentage by volume is 80%, obtains anthocyanidin elution
Eluent is concentrated in vacuo, is freeze-dried to obtain the onion anthocyanidin by liquid；The upper prop stream of ethanol solution during the elution
Speed is 1-1.5 times of column volume/hour.
Further, step (2) enzyme solutions by pectase, cellulase and Pseudomonas cepacia lipase according to matter
Amount is than being 1:1-2:2-3 is mixed to prepare；The enzyme activity of the pectase be 1000u/g-1200u/g, the enzyme activity of cellulase
For 800u/g-1000u/g, the enzyme activity of Pseudomonas cepacia lipase is 1200u/g-1500u/g.
Further, step (2) probiotic solution is pressed by saccharomycete, bacillus subtilis and the galactococcus after screening
It is 1 according to mass ratio:2-3:2-4 is mixed to prepare；The living bacteria count of the yeast liquid is 2.6 × 108~8.9 × 108CFU/g、
The living bacteria count of the bacillus subtilis bacterium solution is 4.5 × 109~9.1 × 109Effective viable bacteria of CFU/g, the milk-globule bacterium solution
Number is 2.6 × 107~6.4 × 107CFU/g。
Further, the screening technique of the saccharomycete is：Yeast strain is inoculated on screening and culturing medium I, in temperature
Spend the saccharomycete after being screened for culture 36h under conditions of 37 DEG C；Wherein, screening and culturing medium I ingredients are：Chitosan 15g, ox
Meat extract 15g, yeast extract 6g, peptone 15g, agar 15g, barbaloin 5g, gumbo polysaccharide 5g, sodium chloride 5g, potassium dihydrogen phosphate 2g,
Magnesium sulfate 0.5g, manganese sulfate 0.25g, Conditions of Onion Juice 30ml, cellulose 10g, pH value 7.1-7.9.
Further, the screening technique of the bacillus subtilis is：Bacillus subtilis strain is inoculated into screening training
It supports on base II, the bacillus subtilis after being screened for 24 hours is cultivated under conditions of temperature is 35 DEG C；Wherein, screening and culturing medium
II ingredients are：Peptone 15g, beef extract 15g, yeast extract 6g, agar 15g, glucose 15g, dragon fruit polysaccharide 5g, salicoside
5g, sodium chloride 5g, potassium dihydrogen phosphate 2g, magnesium sulfate 0.5g, manganese sulfate 0.25g, Conditions of Onion Juice 30ml, cellulose 10g, pH value 6.8-
7.5。
Further, the screening technique of the galactococcus is：Lactococcus strain is inoculated on screening and culturing medium III,
Galactococcus after culture 36h is screened under conditions of temperature is 37 DEG C；Wherein, screening and culturing medium III ingredients are：Agar 15g,
Buffalo's milk 30ml, beef extract 15g, yeast extract 15g, brown sugar 15g, Puerarin 15g, sodium chloride 5g, potassium dihydrogen phosphate 2g, magnesium sulfate
0.5g, manganese sulfate 0.25g, Conditions of Onion Juice 30ml, cellulose 10g, pH value 5.6-6.3.
Further, the percentage by volume of step (3) HFA 134a is 25%.
The present invention has the advantages that：
1st, the application pre-processes onion using biological enzyme, and biological enzyme is false single by pectase, cellulase and onion
Born of the same parents' bacterium lipase is mixed to prepare, wherein, pectase can effectively decompose the pectin composition of onion, and cellulase can effectively decompose onion
Cell wall constituent, Pseudomonas cepacia lipase can effectively for onion cell membrane carry out broken wall, accelerate onion cells in
The precipitation of molten substance, the application also pre-process onion using the probiotic solution after screening, and microorganism can accelerate to ocean
The precipitation of cell anthocyanidin is strengthened in the utilization of green onion cell, still, due to containing more sulfide, vitamin E and Huang in onion
Letones can play bacteriostasis, so as to inhibit microbial activity, in this regard, applicant has developed screening and culturing medium, culture
Containing Conditions of Onion Juice and barbaloin, salicoside, dragon fruit polysaccharide isoreactivity ingredient in base, there can be certain fungistatic effect, through its sieve
It selects the probiotics obtained afterwards that there is stronger antibacterial ability, can effectively decompose onion cells, promote the precipitation of anthocyanidin；This Shen
Subcritical HFA 134a fluid please be additionally use to be extracted, using destroy ozone latent energy value as 01,1,1,2- tetra-
(extractant has environmental-friendly characteristic to fluoroethane.The critical-temperature of HFA 134a is and relatively low critical pressure
Power, larger dipole moment, it is strong to the solvability of polar substances, diffusion coefficient is big, coefficient of viscosity is small the characteristics of, and in liquid
There is good solvent nature with Near The Critical Point, coordinate the biological enzyme of the application, probiotics processing that can effectively improve onion
Anthocyanidin recovery rate and extracted amount, but a large amount of impurity are wherein also had, in order to carry out further purifying to anthocyanidin, be enriched with, this
Application is also further purified extract using macroreticular resin.
【Specific embodiment】
All features or disclosed all methods disclosed in this specification or in the process the step of, except mutually exclusive
Feature and/or step beyond, can combine in any way.
Any feature disclosed in this specification (including any accessory claim, summary), unless specifically stated, each
Feature is an example in a series of equivalent or similar characteristics.
Embodiment 1：
A kind of onion anthocyanidin preparation method is present embodiments provided, this method comprises the following steps：
(1) it will be shredded after the cleaning of ripe onion, removal of impurities, be then 1 according to solid-liquid mass ratio:2 are mixed with water, are put into
Slurries are broken into beater, after filtering filtrate are taken to obtain Conditions of Onion Juice；
(2) digested under 35 DEG C of temperature conditionss according to the addition enzyme solutions of 0.1g/ml, once centrifuged after digesting 8h
Separation takes supernatant to carry out probiotics processing with probiotic solution, carries out secondary centrifuging again supernatant is taken to obtain onion clear liquid；Institute
The centrifugal rotational speed for stating centrifugation is 2000r/min；Centrifugation time is 15min；The additive amount of the probiotics is 0.2g/ml,
Probiotics treatment temperature is 23 DEG C, processing time 18h；The centrifugal rotational speed of the secondary centrifuging is 4000r/min；During centrifugation
Between be 20min；
(3) the onion clear liquid that step (2) obtains is subjected to rotary evaporation and is concentrated to give density as 3.26g/cm3Paste
Then paste is placed in extraction kettle by object, when pressure is 4MPa, static state is carried out to paste with HFA 134a
60min is extracted, then progressively discharges pressure, carries out dynamic extraction, the dynamic extraction time is 50min；The 1 of dynamic extraction,
1,1,2- tetrafluoroethane rate of discharge is 0.7L/min, is left dissolved with the HFA 134a of product after extraction kettle by one
Section is tied with the steel pipe of heating tape, is discharged after being depressurized by pressure reducing valve and obtains onion anthocyanidin crude extract；
(4) HFA 134a being concentrated in vacuo in removal step (3) obtains onion anthocyanidin clear liquid, by anthocyanidin
Clear liquid is adsorbed with large pore resin absorption column, and during absorption, column flow rate is 0.5 times of column volume/hour on anthocyanidin clear liquid, upper prop
Temperature is 30 DEG C, is eluted after the completion of absorption with the ethanol solution that percentage by volume is 80%, obtains anthocyanidin eluent, will
Eluent is concentrated in vacuo, is freeze-dried to obtain the onion anthocyanidin；The upper column flow rate of ethanol solution is 1 during the elution
Times column volume/hour.
Wherein, step (2) enzyme solutions are 1 according to mass ratio by pectase, cellulase and Pseudomonas cepacia lipase:
1:2 are mixed to prepare；The enzyme activity of the pectase is 1000u/g, and the enzyme activity of cellulase is 800u/g, Pseudomonas cepacia
The enzyme activity of lipase is 1200u/g.
Wherein, step (2) probiotic solution by saccharomycete, bacillus subtilis and galactococcus after screening according to quality
Than for 1:2:2 are mixed to prepare；The living bacteria count of the yeast liquid is 2.6 × 108CFU/g, the bacillus subtilis bacterium solution
Living bacteria count be 4.5 × 109CFU/g, the living bacteria count of the milk-globule bacterium solution are 2.6 × 107CFU/g。
1. the screening technique of saccharomycete is in probiotic solution：Yeast strain is inoculated on screening and culturing medium I, in temperature
Spend the saccharomycete after being screened for culture 36h under conditions of 37 DEG C；Wherein, screening and culturing medium I ingredients are：Chitosan 15g, ox
Meat extract 15g, yeast extract 6g, peptone 15g, agar 15g, barbaloin 5g, gumbo polysaccharide 5g, sodium chloride 5g, potassium dihydrogen phosphate 2g,
Magnesium sulfate 0.5g, manganese sulfate 0.25g, Conditions of Onion Juice 30ml, cellulose 10g, pH value 7.1.
2. the screening technique of bacillus subtilis is in probiotic solution：Bacillus subtilis strain is inoculated into screening training
It supports on base II, the bacillus subtilis after being screened for 24 hours is cultivated under conditions of temperature is 35 DEG C；Wherein, screening and culturing medium
II ingredients are：Peptone 15g, beef extract 15g, yeast extract 6g, agar 15g, glucose 15g, dragon fruit polysaccharide 5g, salicoside
5g, sodium chloride 5g, potassium dihydrogen phosphate 2g, magnesium sulfate 0.5g, manganese sulfate 0.25g, Conditions of Onion Juice 30ml, cellulose 10g, pH value 6.8.
3. the screening technique of galactococcus is in probiotic solution：Lactococcus strain is inoculated on screening and culturing medium III,
Galactococcus after culture 36h is screened under conditions of temperature is 37 DEG C；Wherein, screening and culturing medium III ingredients are：Agar 15g,
Buffalo's milk 30ml, beef extract 15g, yeast extract 15g, brown sugar 15g, Puerarin 15g, sodium chloride 5g, potassium dihydrogen phosphate 2g, magnesium sulfate
0.5g, manganese sulfate 0.25g, Conditions of Onion Juice 30ml, cellulose 10g, pH value 5.6.
Wherein, the percentage by volume of step (3) HFA 134a is 25%.
Embodiment 2：
A kind of onion anthocyanidin preparation method is present embodiments provided, this method comprises the following steps：
(1) it will be shredded after the cleaning of ripe onion, removal of impurities, be then 1 according to solid-liquid mass ratio:4 are mixed with water, are put into
Slurries are broken into beater, after filtering filtrate are taken to obtain Conditions of Onion Juice；
(2) digested according to the addition enzyme solutions of 0.5g/ml under 40 DEG C of temperature conditionss, digest 12h after once from
Heart separation takes supernatant to carry out probiotics processing with probiotic solution, carries out secondary centrifuging again supernatant is taken to obtain onion clear liquid；
The centrifugal rotational speed of the centrifugation is 2500r/min；Centrifugation time is 20min；The additive amount of the probiotics is 0.6g/
Ml, probiotics treatment temperature are 27 DEG C, processing time 20h；The centrifugal rotational speed of the secondary centrifuging is 5000r/min；From
The heart time is 30min；
(3) the onion clear liquid that step (2) obtains is subjected to rotary evaporation and is concentrated to give density as 4.15g/cm3Paste
Then paste is placed in extraction kettle by object, when pressure is 5MPa, static state is carried out to paste with HFA 134a
80min is extracted, then progressively discharges pressure, carries out dynamic extraction, the dynamic extraction time is 60min；The 1 of dynamic extraction,
1,1,2- tetrafluoroethane rate of discharge is 0.9L/min, is left dissolved with the HFA 134a of product after extraction kettle by one
Section is tied with the steel pipe of heating tape, is discharged after being depressurized by pressure reducing valve and obtains onion anthocyanidin crude extract；
(4) HFA 134a being concentrated in vacuo in removal step (3) obtains onion anthocyanidin clear liquid, by anthocyanidin
Clear liquid is adsorbed with large pore resin absorption column, and during absorption, column flow rate is 1 times of column volume/hour on anthocyanidin clear liquid, upper column temperature
It spends for 40 DEG C, is eluted after the completion of absorption with the ethanol solution that percentage by volume is 80%, obtain anthocyanidin eluent, will wash
De- liquid is concentrated in vacuo, is freeze-dried to obtain the onion anthocyanidin；The upper column flow rate of ethanol solution is 1.5 during the elution
Times column volume/hour.
Wherein, step (2) enzyme solutions are 1 according to mass ratio by pectase, cellulase and Pseudomonas cepacia lipase:
2:3 are mixed to prepare；The enzyme activity of the pectase is 1200u/g, and the enzyme activity of cellulase is 1000u/g, onion vacation unit cell
The enzyme activity of bacterium lipase is 1500u/g.
Wherein, step (2) probiotic solution by saccharomycete, bacillus subtilis and galactococcus after screening according to quality
Than for 1:3:4 are mixed to prepare；The living bacteria count of the yeast liquid is 8.9 × 108CFU/g, the bacillus subtilis bacterium solution
Living bacteria count be 9.1 × 109CFU/g, the living bacteria count of the milk-globule bacterium solution are 6.4 × 107CFU/g。
1. the screening technique of saccharomycete is in probiotic solution：Yeast strain is inoculated on screening and culturing medium I, in temperature
Spend the saccharomycete after being screened for culture 36h under conditions of 37 DEG C；Wherein, screening and culturing medium I ingredients are：Chitosan 15g, ox
Meat extract 15g, yeast extract 6g, peptone 15g, agar 15g, barbaloin 5g, gumbo polysaccharide 5g, sodium chloride 5g, potassium dihydrogen phosphate 2g,
Magnesium sulfate 0.5g, manganese sulfate 0.25g, Conditions of Onion Juice 30ml, cellulose 10g, pH value 7.9.
2. the screening technique of bacillus subtilis is in probiotic solution：Bacillus subtilis strain is inoculated into screening training
It supports on base II, the bacillus subtilis after being screened for 24 hours is cultivated under conditions of temperature is 35 DEG C；Wherein, screening and culturing medium
II ingredients are：Peptone 15g, beef extract 15g, yeast extract 6g, agar 15g, glucose 15g, dragon fruit polysaccharide 5g, salicoside
5g, sodium chloride 5g, potassium dihydrogen phosphate 2g, magnesium sulfate 0.5g, manganese sulfate 0.25g, Conditions of Onion Juice 30ml, cellulose 10g, pH value 7.5.
3. the screening technique of galactococcus is in probiotic solution：Lactococcus strain is inoculated on screening and culturing medium III,
Galactococcus after culture 36h is screened under conditions of temperature is 37 DEG C；Wherein, screening and culturing medium III ingredients are：Agar 15g,
Buffalo's milk 30ml, beef extract 15g, yeast extract 15g, brown sugar 15g, Puerarin 15g, sodium chloride 5g, potassium dihydrogen phosphate 2g, magnesium sulfate
0.5g, manganese sulfate 0.25g, Conditions of Onion Juice 30ml, cellulose 10g, pH value 6.3.
Wherein, the percentage by volume of step (3) HFA 134a is 25%.
Embodiment 3：
A kind of onion anthocyanidin preparation method is present embodiments provided, this method comprises the following steps：
(1) it will be shredded after the cleaning of ripe onion, removal of impurities, be then 1 according to solid-liquid mass ratio:3 are mixed with water, are put into
Slurries are broken into beater, after filtering filtrate are taken to obtain Conditions of Onion Juice；
(2) digested according to the addition enzyme solutions of 0.4g/ml under 37 DEG C of temperature conditionss, digest 10h after once from
Heart separation takes supernatant to carry out probiotics processing with probiotic solution, carries out secondary centrifuging again supernatant is taken to obtain onion clear liquid；
The centrifugal rotational speed of the centrifugation is 2300r/min；Centrifugation time is 17min；The additive amount of the probiotics is 0.4g/
Ml, probiotics treatment temperature are 25 DEG C, processing time 19h；The centrifugal rotational speed of the secondary centrifuging is 4500r/min；From
The heart time is 25min；
(3) the onion clear liquid that step (2) obtains is subjected to rotary evaporation and is concentrated to give density as 3.89g/cm3Paste
Then paste is placed in extraction kettle by object, when pressure is 4.5MPa, paste is carried out with HFA 134a quiet
State extracts 70min, then progressively discharges pressure, carries out dynamic extraction, and the dynamic extraction time is 55min；Dynamic extraction
HFA 134a rate of discharge be 0.8L/min, dissolved with product HFA 134a leave extraction kettle after pass through
One section of steel pipe for being tied with heating tape discharges after being depressurized by pressure reducing valve and obtains onion anthocyanidin crude extract；
(4) HFA 134a being concentrated in vacuo in removal step (3) obtains onion anthocyanidin clear liquid, by anthocyanidin
Clear liquid is adsorbed with large pore resin absorption column, and during absorption, column flow rate is 0.7 times of column volume/hour on anthocyanidin clear liquid, upper prop
Temperature is 35 DEG C, is eluted after the completion of absorption with the ethanol solution that percentage by volume is 80%, obtains anthocyanidin eluent, will
Eluent is concentrated in vacuo, is freeze-dried to obtain the onion anthocyanidin；The upper column flow rate of ethanol solution is during the elution
1.2 times of column volume/hours.
Wherein, step (2) enzyme solutions are 1 according to mass ratio by pectase, cellulase and Pseudomonas cepacia lipase:
1:3 are mixed to prepare；The enzyme activity of the pectase is 1100u/g, and the enzyme activity of cellulase is 900u/g, Pseudomonas cepacia
The enzyme activity of lipase is 1300u/g.
Wherein, step (2) probiotic solution by saccharomycete, bacillus subtilis and galactococcus after screening according to quality
Than for 1:2:3 are mixed to prepare；The living bacteria count of the yeast liquid is 4.9 × 108CFU/g, the bacillus subtilis bacterium solution
Living bacteria count be 6.1 × 109CFU/g, the living bacteria count of the milk-globule bacterium solution are 4.4 × 107CFU/g。
1. the screening technique of saccharomycete is in probiotic solution：Yeast strain is inoculated on screening and culturing medium I, in temperature
Spend the saccharomycete after being screened for culture 36h under conditions of 37 DEG C；Wherein, screening and culturing medium I ingredients are：Chitosan 15g, ox
Meat extract 15g, yeast extract 6g, peptone 15g, agar 15g, barbaloin 5g, gumbo polysaccharide 5g, sodium chloride 5g, potassium dihydrogen phosphate 2g,
Magnesium sulfate 0.5g, manganese sulfate 0.25g, Conditions of Onion Juice 30ml, cellulose 10g, pH value 7.5.
2. the screening technique of bacillus subtilis is in probiotic solution：Bacillus subtilis strain is inoculated into screening training
It supports on base II, the bacillus subtilis after being screened for 24 hours is cultivated under conditions of temperature is 35 DEG C；Wherein, screening and culturing medium
II ingredients are：Peptone 15g, beef extract 15g, yeast extract 6g, agar 15g, glucose 15g, dragon fruit polysaccharide 5g, salicoside
5g, sodium chloride 5g, potassium dihydrogen phosphate 2g, magnesium sulfate 0.5g, manganese sulfate 0.25g, Conditions of Onion Juice 30ml, cellulose 10g, pH value 7.2.
3. the screening technique of galactococcus is in probiotic solution：Lactococcus strain is inoculated on screening and culturing medium III,
Galactococcus after culture 36h is screened under conditions of temperature is 37 DEG C；Wherein, screening and culturing medium III ingredients are：Agar 15g,
Buffalo's milk 30ml, beef extract 15g, yeast extract 15g, brown sugar 15g, Puerarin 15g, sodium chloride 5g, potassium dihydrogen phosphate 2g, magnesium sulfate
0.5g, manganese sulfate 0.25g, Conditions of Onion Juice 30ml, cellulose 10g, pH value 5.8.
Wherein, the percentage by volume of step (3) HFA 134a is 25%.
Control group 1：
This control group is handled onion without biological enzyme, and other preparation methods are identical with embodiment 1.
Control group 2：
This control group is handled onion without using probiotics bacterial liquid, and other preparation methods are identical with embodiment 1.
Control group 3：
For this control group using probiotics bacterial liquid without Screening Treatment, other preparation methods are identical with embodiment 1.
Control group 4：
It is 1 according to mass ratio after screening that the probiotics bacterial liquid of this control group, which uses saccharomycete and galactococcus,:2 mixing systems
, other screening techniques, preparation method are identical with embodiment 1.
Control group 5：
It is 1 according to mass ratio after screening that the probiotics bacterial liquid of this control group, which uses saccharomycete and bacillus subtilis,:2
It is mixed to prepare, other screening techniques, preparation method are identical with embodiment 1.
Control group 6：
It is 1 according to mass ratio after screening that the probiotics bacterial liquid of this control group, which uses galactococcus and bacillus subtilis,:1
It is mixed to prepare, other screening techniques, preparation method are identical with embodiment 1.
Control group 7：
The percentage by volume of the HFA 134a of this control group is 20%, and other preparation methods and embodiment 1 are complete
It is exactly the same.
Control group 8：
The percentage by volume of the HFA 134a of this control group is 15%, and other preparation methods and embodiment 1 are complete
It is exactly the same.
Control group 9：
The percentage by volume of the HFA 134a of this control group is 30%, and other preparation methods and embodiment 1 are complete
It is exactly the same.
Control group 10：
The percentage by volume of the HFA 134a of this control group is 35%, and other preparation methods and embodiment 1 are complete
It is exactly the same.
Control group 11：
HFA 134a solution is replaced with ethanol solution by this control group, and other preparation methods and embodiment 1 are complete
It is exactly the same.
Test experiments：
It measures in embodiment 1-3 and control group 1-11, yield, recovery rate and the DPPH free radical scavenging activities of anthocyanidin,
Concrete condition is shown in Table 1：
Table 1
As seen from the above table, the yield of the anthocyanidin of embodiment 1-3, recovery rate and DPPH free radical scavenging activities are above pair
According to a group 1-11, wherein, control group 2, the anthocyanidin yield of control group 7-11 and recovery rate are lower than other control groups, illustrate the application
Probiotics processing, percentage by volume be 25% HFA 134a solution sub-critical extraction can effectively improve onion
Anthocyanidin yield and recovery rate.
In conclusion using the present invention method prepare anthocyanidin can safely, effectively improve onion anthocyanidin yield and
Recovery rate.
Embodiment described above only expresses the several embodiments of the present invention, and description is more specific and detailed, but simultaneously
Cannot limitation of the scope of the invention therefore be interpreted as.It should be pointed out that for those of ordinary skill in the art,
Without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the protection model of the present invention
It encloses.Therefore, protection scope of the present invention should be determined by the appended claims.
Claims (7)
1. a kind of onion anthocyanidin preparation method, which is characterized in that described method includes following steps：
(1) it will be shredded after the cleaning of ripe onion, removal of impurities, be then 1 according to solid-liquid mass ratio:2-4 is mixed with water, is put into and is beaten
Slurries are broken into pulp grinder, after filtering filtrate are taken to obtain Conditions of Onion Juice；
(2) digested according to the addition enzyme solutions of 0.1g/ml-0.5g/ml under 35-40 DEG C of temperature conditionss, digest 8h-
It is once centrifuged after 12h and supernatant is taken to carry out probiotics processing with probiotic solution, secondary centrifuging is carried out again supernatant is taken to obtain
To onion clear liquid；The centrifugal rotational speed of the centrifugation is 2000r/min-2500r/min；Centrifugation time is 15min-20min；
The additive amount of the probiotics is 0.2g/ml-0.6g/ml, and probiotics treatment temperature is 23-27 DEG C, processing time 18h-
20h；The centrifugal rotational speed of the secondary centrifuging is 4000r/min-5000r/min；Centrifugation time is 20min-30min；
(3) the onion clear liquid that step (2) obtains is subjected to rotary evaporation and is concentrated to give density as 3.26g/cm3-4.15g/cm3's
Then paste is placed in extraction kettle by paste, pressure be 4-5MPa when, with HFA 134a to paste into
Then row static extracting 60-80min progressively discharges pressure, carry out dynamic extraction, and the dynamic extraction time is 50min-
60min；The HFA 134a rate of discharge of dynamic extraction is 0.7-0.9L/min, dissolved with the 1 of product, 1,1,2- tetrafluoro
Ethane leaves the steel pipe for being tied with heating tape by one section after extraction kettle, after being depressurized by pressure reducing valve discharge obtain onion anthocyanidin and slightly carry
Liquid；
(4) HFA 134a being concentrated in vacuo in removal step (3) obtains onion anthocyanidin clear liquid, by anthocyanidin clear liquid
It is adsorbed with large pore resin absorption column, during absorption, column flow rate is 0.5-1 times of column volume/hour on anthocyanidin clear liquid, upper column temperature
It spends for 30-40 DEG C, is eluted after the completion of absorption with the ethanol solution that percentage by volume is 80%, obtain anthocyanidin eluent,
Eluent is concentrated in vacuo, is freeze-dried to obtain the onion anthocyanidin；The upper column flow rate of ethanol solution during the elution
For 1-1.5 times of column volume/hour.
2. onion anthocyanidin preparation method according to claim 1, which is characterized in that step (2) enzyme solutions are by fruit
Glue enzyme, cellulase and Pseudomonas cepacia lipase are 1 according to mass ratio:1-2:2-3 is mixed to prepare；The enzyme of the pectase
Vigor is 1000u/g-1200u/g, and the enzyme activity of cellulase is 800u/g-1000u/g, the enzyme of Pseudomonas cepacia lipase
Vigor is 1200u/g-1500u/g.
3. onion anthocyanidin preparation method according to claim 1, which is characterized in that step (2) probiotic solution
By saccharomycete, bacillus subtilis and the galactococcus after screening according to mass ratio be 1:2-3:2-4 is mixed to prepare；The saccharomycete
The living bacteria count of liquid is 2.6 × 108~8.9 × 108CFU/g, the living bacteria count of the bacillus subtilis bacterium solution for 4.5 ×
109~9.1 × 109CFU/g, the living bacteria count of the milk-globule bacterium solution are 2.6 × 107~6.4 × 107CFU/g。
4. onion anthocyanidin preparation method according to claim 3, which is characterized in that the screening technique of the saccharomycete
For：Yeast strain is inoculated on screening and culturing medium I, the ferment after culture 36h is screened under conditions of temperature is 37 DEG C
Female bacterium；Wherein, screening and culturing medium I ingredients are：Chitosan 15g, beef extract 15g, yeast extract 6g, peptone 15g, agar 15g, reed
Luxuriant growth glucoside 5g, gumbo polysaccharide 5g, sodium chloride 5g, potassium dihydrogen phosphate 2g, magnesium sulfate 0.5g, manganese sulfate 0.25g, Conditions of Onion Juice 30ml, it is fine
Tie up element 10g, pH value 7.1-7.9.
5. onion anthocyanidin preparation method according to claim 3, which is characterized in that the screening of the bacillus subtilis
Method is：Bacillus subtilis strain is inoculated on screening and culturing medium II, cultivates under conditions of temperature is 35 DEG C and obtains for 24 hours
Bacillus subtilis after screening；Wherein, screening and culturing medium II ingredients are：Peptone 15g, beef extract 15g, yeast extract 6g, fine jade
Fat 15g, glucose 15g, dragon fruit polysaccharide 5g, salicoside 5g, sodium chloride 5g, potassium dihydrogen phosphate 2g, magnesium sulfate 0.5g, manganese sulfate
0.25g, Conditions of Onion Juice 30ml, cellulose 10g, pH value 6.8-7.5.
6. onion anthocyanidin preparation method according to claim 3, which is characterized in that the screening technique of the galactococcus
For：Lactococcus strain is inoculated on screening and culturing medium III, after culture 36h is screened under conditions of temperature is 37 DEG C
Galactococcus；Wherein, screening and culturing medium III ingredients are：Agar 15g, buffalo's milk 30ml, beef extract 15g, yeast extract 15g, brown sugar
15g, Puerarin 15g, sodium chloride 5g, potassium dihydrogen phosphate 2g, magnesium sulfate 0.5g, manganese sulfate 0.25g, Conditions of Onion Juice 30ml, cellulose
10g, pH value 5.6-6.3.
7. onion anthocyanidin preparation method according to claim 2, which is characterized in that the step (3) 1,1,1,2- tetra-
The percentage by volume of fluoroethane is 25%.
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| CN107033117A (en) * | 2017-03-31 | 2017-08-11 | 启东市清清蔬果农地股份专业合作社 | A kind of method that anthocyanidin is extracted from rape |
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| CN107033117A (en) * | 2017-03-31 | 2017-08-11 | 启东市清清蔬果农地股份专业合作社 | A kind of method that anthocyanidin is extracted from rape |
| CN107089964A (en) * | 2017-03-31 | 2017-08-25 | 启东市清清蔬果农地股份专业合作社 | A kind of baby cabbage anthocyanidin extracting method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115812604A (en) * | 2023-01-06 | 2023-03-21 | 东北农业大学 | Bradysia odoriphaga regeneration culture medium and preparation method thereof |
| CN115812604B (en) * | 2023-01-06 | 2023-08-29 | 东北农业大学 | Leek regeneration medium and preparation method thereof |
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Application publication date: 20180529 |