CN109666083A - A kind of plant polyose extraction element and its method for extracting bletilla polysaccharide - Google Patents
A kind of plant polyose extraction element and its method for extracting bletilla polysaccharide Download PDFInfo
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- CN109666083A CN109666083A CN201910095591.8A CN201910095591A CN109666083A CN 109666083 A CN109666083 A CN 109666083A CN 201910095591 A CN201910095591 A CN 201910095591A CN 109666083 A CN109666083 A CN 109666083A
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- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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Abstract
This programme discloses a kind of plant polyose extraction element in bletilla polysaccharide extractive technique field, including extracts case, and the inside for extracting case is closed cavity, is rotatably connected to support board in cavity, is evenly equipped with filter opening on support board;Wall body is equipped with storage chamber;Stirring rod is also rotatably connected on wall body;It is equipped with crushing chamber in the side wall of cavity, material residue channel is equipped between crushing chamber and the cavity;It is equipped with crushing bar in crushing chamber, crushes bar and is equipped with screw propulsion piece, crushes one end that bar is located at outside extraction case and is fixedly connected with motor, be fixedly connected with gear on the output shaft of motor;It extracts and is additionally provided with feed opening on the wall body of case, feed opening is located at the surface of crushing chamber;Push plate is slidably connected in feed opening, the top of push plate is fixedly connected with push rod, and push rod is connected with elevating lever, and rack gear is fixed on elevating lever, and rack gear is engaged with the gear.Plant polyose extraction element in this programme is remarkably improved the recovery rate of bletilla polysaccharide.
Description
Technical field
The invention belongs to bletilla polysaccharide extractive technique field, in particular to a kind of plant polyose extraction element and its for mentioning
The method for taking bletilla polysaccharide.
Background technique
Bletilla polysaccharide is a kind of glutinous polysaccharide mentioned from the bletilla striata, not only has hemostasia effect, while being also that common preparation is auxiliary
Material, can be used as the host material of a variety of dosage forms, has the function of mucosa adhesion, bonding, sustained release, emulsification, packing etc., be a kind of good
Pharmaceutical carrier.The extracting method of bletilla polysaccharide mainly has solvent extraction method, sour formulation, alkaline extraction, enzyme formulation, ultrafiltration, surpasses
Sound extraction, Microwave Extraction, CO2Supercritical extract etc..Method for extraction and purification and process conditions seriously affect the property of products therefrom
And effect.Wherein solvent extraction method does not need special installation, at low cost, is most common method;And in solvent extraction method, and because
Influence when for water as solvent extraction to the property of bletilla polysaccharide and effect is minimum, therefore is relatively conventional using water as solvent
A kind of solvent extraction mode.
In current plant polyose extraction process, impregnated into the water after crushing raw material mostly, be filtered,
Concentration filters and then obtains plant polyose.But first raw material is crushed, it is then impregnated, is operated comparatively laborious again.Together
Shi Caiyong water is extracted as solvent, is wrapped in the polysaccharide in fiber and in albumen and is difficult to dissolve out, so that plant is more
The recovery rate of sugar is lower.
Summary of the invention
The invention is intended to provide a kind of plant polyose extraction element, in order to the extraction of plant polyose, while the plant is utilized
The recovery rate of object Polyose extraction device raising bletilla polysaccharide.
One of this programme plant polyose extraction element, including case is extracted, the inside for extracting case is closed cavity, empty
It is intracavitary to be rotatably connected to support board, filter opening is evenly equipped on support board;The wall body of cavity top be equipped with for store protease and
The storage chamber of cellulase;The stirring rod for stirring and squeezing filter residue is also rotatably connected on the wall body of cavity top;Cavity
Side wall in be equipped with closed crushing chamber, between crushing chamber and the cavity be equipped with material residue channel;It is equipped in crushing chamber and crushes bar,
It crushes bar and is equipped with screw propulsion piece, crush bar and extended through out outside the extraction case far from one end of the cavity, crush bar
It is fixedly connected with the motor of its rotation of driving positioned at the one end extracted outside case, is fixedly connected with gear on the output shaft of motor;Institute
It states and is additionally provided with feed opening on the wall body for extracting case, feed opening is located at the surface of crushing chamber;Push plate is slidably connected in feed opening,
The outer rim of push plate and the side wall of feed opening fit, and the top of push plate is fixedly connected with push rod, and push rod is connected with elevating lever, lifting
The rack gear being vertically arranged is fixedly connected on bar, rack gear is engaged with the gear.
The working principle of this programme and its utility model has the advantages that in use, the material for needing to extract polysaccharide is put into feed opening
It is interior, start motor, motor, which drives, crushes bar rotation, and the screw propulsion piece crushed on bar crush and by it to material residue to material
Channel promotes, and finally material residue is pushed into cavity.During motor rotation, gear rotation band carry-over bar declines, in turn
It drives elevating lever and push rod connected to it to decline, realizes pushing of the push plate to material, until push plate reaches the bottom of feed opening,
Avoid residual material in feed opening.After material residue enters cavity, protease and cellulase are launched into cavity, and pass through stirring
Bar stirs material residue, so that albumen and cellulose decomposition help to improve the extraction of polysaccharide so that its internal polysaccharide releases
Rate.After albumen and cellulose decomposition in material residue, support board is rotated, the material residue on support board is made to enter lower section;It simultaneously can be to
Clear water is launched in cavity, clear water can further dissolve out the polysaccharide in material residue.After polysaccharide dissolution, pass through the discharge hole being connected to cavity
Taking-up.
Further, the wall body for extracting case is equipped with water tank, and water tank is communicated with water pipe, and water pipe connects far from one end of water tank
Lead to the crushing chamber, water pipe is equipped with water control valve.By setting water tank, water control valve and water pipe, powder is carried out to material crushing bar
It is broken and push during, water tank can be filled the water into crushing chamber, so while to crushing material to the polysaccharide in material into
Row dissolution, is conducive to the extraction of polysaccharide.
Further, the bottom of the storage chamber is equipped with the fluid hole that be connected to cavity, equipped with chock plug in fluid hole, on chock plug
It is fixedly connected with bull stick, one end of bull stick is extended outside the extraction case and is threadedly coupled with case is extracted.By setting chock plug and
Bull stick can realize dispensing protease or cellulase into cavity by rotating bull stick;Using more convenient.
Further, the bottom of the cavity is equipped with heating sheet.By the way that heating sheet is arranged, the water in cavity can be added
Heat is conducive to the dissolution of polysaccharide.
The method that plant polyose extraction element is used to extract bletilla polysaccharide, comprising the following steps:
Step 1: the bletilla striata is put into from feed opening, starts motor, and control rate of feeding is 10~12kg/h;It opens simultaneously
Water control valve is opened, holding solid-liquid ratio is 1:10~1:7;
Step 2: during the bletilla striata is launched, every 10~15min launches a protease and cellulase to support board, launches
When protease and cellulase, bletilla striata material residue is stirred evenly by stirring rod;The weight of the injected volume and the bletilla striata of protease and cellulase
Amount is than being 1:80~1:60;
Step 3: the heating temperature for keeping heating sheet is 35~50 DEG C;
Step 4: after bletilla striata material residue is completely into 1~2h of support board is arrived, rotation support board entirely falls in bletilla striata material residue
Lower section;Fall behind under bletilla striata material residue and stands 2~3h;
Step 5: the material after standing in cavity is taken out, and 95% ethyl alcohol is added after cooling under stirring and makes to contain
Alcohol amount reaches 55~60%;It is then allowed to stand 5~8h;
Step 6: the material after standing is filtered, then vacuum freeze drying obtains bletilla polysaccharide.
This programme the utility model has the advantages that by motor control rate of feeding be 10~12kg/h and control solid-liquid ratio be 1:10 extremely
1:7 is conducive to coming into full contact with for water and the bletilla striata, guarantees the initial dissolution rate of bletilla polysaccharide.Add subsequently through into bletilla striata material residue
Add protease and cellulase, is fully dissolved out the polysaccharide being wrapped in albumen and cellulose, is remarkably improved bletilla polysaccharide
Dissolution rate, and be 35~50 DEG C by temperature setting, while guaranteeing protease and cellulase activity, it is remarkably improved the bletilla striata
The dissolution rate of polysaccharide.Subsequent addition ethyl alcohol further reduced the purity that mixed albumen in bletilla polysaccharide improves bletilla polysaccharide.
Detailed description of the invention
Fig. 1 is a kind of structural schematic diagram of plant polyose extraction element of the present invention.
Specific embodiment
It is further described below by specific embodiment:
Appended drawing reference in Figure of description includes: to extract case 1, bull stick 2, protease chamber 3, rotating bar 4, cover board 5, fiber
Plain enzyme chamber 6, feed opening 7, push rod 8, elevating lever 9, limit plate 10, push plate 11, rack gear 12, servo motor 13, gear 14, bearing
15, water tank 16, water control valve 17, water pipe 18, crush bar 19, screw propulsion piece 20, material residue channel 21, stirring rod 22, support board 23,
Filter opening 24, cavity 25, plug 26, turning rod 27, heating sheet 28.
A kind of plant polyose extraction element, as shown in Fig. 1: including extracting case 1, the inside for extracting case 1 is closed sky
Chamber 25 is rotatably connected to support board 23 in cavity 25, and support board 23 is fixedly connected with turning rod 27, and turning rod 27 extends to extraction
Outside case 1;Filter opening 24 is evenly equipped on support board 23;The bottom of cavity is equipped with heating sheet 28;The wall body at 25 top of cavity, which is equipped with, to be used
In the storage chamber of storage protease and cellulase;The bottom of storage chamber is equipped with the fluid hole that is connected to cavity 25, in fluid hole
Equipped with chock plug, be fixedly connected with bull stick 2 on chock plug, one end of bull stick 2 extend the extraction case 1 it is outer and with extract 1 screw thread of case
Connection;Storage chamber includes protease chamber 3 and cellulase chamber 6;It is also rotatably connected on the wall body at 25 top of cavity for stirring
With the stirring rod 22 for squeezing filter residue, rotating bar 4 is fixedly connected on stirring rod 22, rotating bar 4, which extends to, to be extracted outside case 1;Cavity
25 bottom is equipped with discharge port, is equipped with plug 26 in discharge port;Be equipped with closed crushing chamber in the side wall of cavity 25, crushing chamber with
Material residue channel 21 is equipped between the cavity 25;It is equipped in crushing chamber and crushes bar 19, crushed bar 19 and be equipped with screw propulsion piece 20,
It crushes bar 19 to extend through out outside the extraction case 1 far from one end of the cavity 25, extracts and be equipped with bearing in the wall body of case 1
15, it crushes bar 19 and is fixedly connected on bearing 15, crush one end that bar 19 is located at outside extraction case 1 and be fixedly connected with its rotation of driving
Servo motor 13, be fixedly connected with gear 14 on the output shaft of servo motor 13;It is described extract case 1 wall body on be additionally provided with into
Expect hole 7, feed opening 7 is located at the surface of crushing chamber;Push plate 11, the outer rim and charging of push plate 11 are slidably connected in feed opening 7
The side wall in hole 7 fits, and the top of push plate 11 is fixedly connected with push rod 8, and push rod 8 is connected with elevating lever 9, fixes on elevating lever 9
It is connected with the rack gear 12 being vertically arranged, rack gear 12 is engaged with the gear 14;The top for extracting case 1 is equipped with the lid of sealed storage chamber
Plate 5;The outer wall for extracting case 1 is equipped with limit plate 10, and lifter plate is slidably connected on limit plate 10;The wall body for extracting case 1 is equipped with
Water tank 16, water tank 16 is interior to be equipped with water pump, and the outlet end of water pump is communicated with water pipe 18, and water pipe 18 is connected to institute far from one end of water tank 16
Crushing chamber is stated, water pipe 18 is equipped with water control valve 17;Servo motor 13 is fixedly connected on water tank 16.
Embodiment 1:
A kind of method that plant polyose extraction element is used to extract bletilla polysaccharide, comprising the following steps:
Step 1: the bletilla striata is put into from feed opening 7, starts motor, and control rate of feeding is 11kg/h;Control is opened simultaneously
Water valve 17, holding solid-liquid ratio are 1:10;
Step 2: during the bletilla striata is launched, every 15min launches a protease and cellulase to support board 23, launches egg
When white enzyme and cellulase, bletilla striata material residue is stirred evenly by stirring rod 22;The weight of the injected volume and the bletilla striata of protease and cellulase
Amount is than being 1:70;
Step 3: the heating temperature for keeping heating sheet 28 is 38 DEG C;
Step 4: after bletilla striata material residue is completely into 23 2h of support board is arrived, rotation support board 23 entirely falls in bletilla striata material residue
To lower section;Fall behind under bletilla striata material residue and stands 3h;
Step 5: the material after standing in cavity 25 is taken out, and 95% ethyl alcohol is added after cooling under stirring and makes
Alcohol content reaches 60%;It is then allowed to stand 6h;
Step 6: the material after standing is filtered, then vacuum freeze drying obtains bletilla polysaccharide.
Embodiment 2:
A kind of method that plant polyose extraction element is used to extract bletilla polysaccharide, comprising the following steps:
Step 1: the bletilla striata is put into from feed opening 7, starts motor, and control rate of feeding is 12kg/h;Control is opened simultaneously
Water valve 17, holding solid-liquid ratio are 1:7;
Step 2: during the bletilla striata is launched, every 13min launches a protease and cellulase to support board 23, launches egg
When white enzyme and cellulase, bletilla striata material residue is stirred evenly by stirring rod 22;The weight of the injected volume and the bletilla striata of protease and cellulase
Amount is than being 1:80;
Step 3: the heating temperature for keeping heating sheet 28 is 40 DEG C;
Step 4: after bletilla striata material residue is completely into 23 1h of support board is arrived, rotation support board 23 entirely falls in bletilla striata material residue
To lower section;Fall behind under bletilla striata material residue and stands 2h;
Step 5: the material after standing in cavity 25 is taken out, and 95% ethyl alcohol is added after cooling under stirring and makes
Alcohol content reaches 55%;It is then allowed to stand 8h;
Step 6: the material after standing is filtered, then vacuum freeze drying obtains bletilla polysaccharide.
Embodiment 3:
A kind of method that plant polyose extraction element is used to extract bletilla polysaccharide, comprising the following steps:
Step 1: the bletilla striata is put into from feed opening 7, starts motor, and control rate of feeding is 10~12kg/h;It opens simultaneously
Water control valve 17 is opened, holding solid-liquid ratio is 1:10~1:7, and bletilla striata material residue enters directly into 25 bottom of cavity;
Step 2: the heating temperature for keeping heating sheet 28 is 35~50 DEG C;Stand 12~13h;
Step 3: the material after standing in cavity 25 is taken out, and 95% ethyl alcohol is added after cooling under stirring and makes
Alcohol content reaches 60%;It is then allowed to stand 6h;
Step 4: the material after standing is filtered, then vacuum freeze drying obtains bletilla polysaccharide.
Experimental comparison: total reducing sugar is measured using Anthrone-sulfuricacid method, 3,5- dinitrosalicylic acid system measures reduced sugar;Polysaccharide=
Total reducing sugar-reduced sugar, with glucose as a standard product (correction coefficient 0.9).Using the Coomassie brilliant G-250 method of Bradford, with
Bovine serum albumin(BSA) is that standard protein measures soluble protein.Data are as shown in the table:
As can be seen from the table, the recovery rate of 1~embodiment of embodiment, 2 bletilla polysaccharide is significantly higher than embodiment 3, while egg
White matter content is also significantly reduced.
Claims (5)
1. a kind of plant polyose extraction element, it is characterised in that: including extracting case, the inside for extracting case is closed cavity, empty
It is intracavitary to be rotatably connected to support board, filter opening is evenly equipped on support board;The wall body of cavity top be equipped with for store protease and
The storage chamber of cellulase;The stirring rod for stirring and squeezing filter residue is also rotatably connected on the wall body of cavity top;Cavity
Side wall in be equipped with closed crushing chamber, between crushing chamber and the cavity be equipped with material residue channel;It is equipped in crushing chamber and crushes bar,
It crushes bar and is equipped with screw propulsion piece, crush bar and extended through out outside the extraction case far from one end of the cavity, crush bar
It is fixedly connected with the motor of its rotation of driving positioned at the one end extracted outside case, is fixedly connected with gear on the output shaft of motor;Institute
It states and is additionally provided with feed opening on the wall body for extracting case, feed opening is located at the surface of crushing chamber;Push plate is slidably connected in feed opening,
The outer rim of push plate and the side wall of feed opening fit, and the top of push plate is fixedly connected with push rod, and push rod is connected with elevating lever, lifting
The rack gear being vertically arranged is fixedly connected on bar, rack gear is engaged with the gear.
2. a kind of plant polyose extraction element according to claim 1, it is characterised in that: set on the wall body for extracting case
There is water tank, water tank is communicated with water pipe, and water pipe is connected to the crushing chamber far from one end of water tank, and water pipe is equipped with water control valve.
3. a kind of plant polyose extraction element according to claim 2, it is characterised in that: the bottom of the storage chamber is equipped with
The fluid hole being connected to cavity, fluid hole is interior to be equipped with chock plug, bull stick is fixedly connected on chock plug, one end of bull stick is extended described
It extracts outside case and is threadedly coupled with case is extracted.
4. a kind of plant polyose extraction element according to claim 3, it is characterised in that: the bottom of the cavity, which is equipped with, to be added
Backing.
5. the method that a kind of plant polyose extraction element according to claim 4 is used to extract bletilla polysaccharide, feature exist
In: the following steps are included:
Step 1: the bletilla striata is put into from feed opening, starts motor, and control rate of feeding is 10~12kg/h;Control is opened simultaneously
Water valve, holding solid-liquid ratio are 1:10~1:7;
Step 2: during the bletilla striata is launched, every 10~15min launches a protease and cellulase to support board, launches albumen
When enzyme and cellulase, bletilla striata material residue is stirred evenly by stirring rod;The weight ratio of the injected volume and the bletilla striata of protease and cellulase
For 1:80~1:60;
Step 3: the heating temperature for keeping heating sheet is 35~50 DEG C;
Step 4: after bletilla striata material residue is completely into 1~2h of support board is arrived, rotation support board entirely falls in down bletilla striata material residue
Side;Fall behind under bletilla striata material residue and stands 2~3h;
Step 5: the material after standing in cavity is taken out, and 95% ethyl alcohol is added after cooling under stirring and makes alcohol content
Reach 55~60%;It is then allowed to stand 5~8h;
Step 6: the material after standing is filtered, then vacuum freeze drying obtains bletilla polysaccharide.
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CN201910095591.8A CN109666083B (en) | 2019-01-31 | 2019-01-31 | Plant polysaccharide extraction device and method for extracting bletilla striata polysaccharide by using plant polysaccharide extraction device |
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CN201910095591.8A CN109666083B (en) | 2019-01-31 | 2019-01-31 | Plant polysaccharide extraction device and method for extracting bletilla striata polysaccharide by using plant polysaccharide extraction device |
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Cited By (1)
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