CN106821803B - A kind of Rhizoma Chuanxiong essential oil composition and its preparation method and application - Google Patents

A kind of Rhizoma Chuanxiong essential oil composition and its preparation method and application Download PDF

Info

Publication number
CN106821803B
CN106821803B CN201710070862.5A CN201710070862A CN106821803B CN 106821803 B CN106821803 B CN 106821803B CN 201710070862 A CN201710070862 A CN 201710070862A CN 106821803 B CN106821803 B CN 106821803B
Authority
CN
China
Prior art keywords
essential oil
rhizoma chuanxiong
parts
oil composition
chuanxiong essential
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201710070862.5A
Other languages
Chinese (zh)
Other versions
CN106821803A (en
Inventor
周厚成
胡昌江
黄宇
李文兵
喻荣平
林娟
张彩虹
何芳
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sichuan New Green Pharmaceutical Technology Development Co Ltd
Original Assignee
Sichuan New Green Pharmaceutical Technology Development Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sichuan New Green Pharmaceutical Technology Development Co Ltd filed Critical Sichuan New Green Pharmaceutical Technology Development Co Ltd
Priority to CN201710070862.5A priority Critical patent/CN106821803B/en
Publication of CN106821803A publication Critical patent/CN106821803A/en
Application granted granted Critical
Publication of CN106821803B publication Critical patent/CN106821803B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
    • A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
    • A61K8/34—Alcohols
    • A61K8/345—Alcohols containing more than one hydroxy group
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
    • A61Q17/02—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings containing insect repellants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Birds (AREA)
  • Dermatology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical & Material Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Emergency Medicine (AREA)
  • Cosmetics (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

The invention discloses a kind of Rhizoma Chuanxiong essential oil compositions and its preparation method and application.In parts by weight, which includes following components: 45~55 parts of Rhizoma Chuanxiong essential oil, 43~50 parts of glycerol, 5~10 parts of peppermint oil and 0.05~0.1 part of preservative.Preparation method is the following steps are included: weigh each component according to weight part ratio；Each component in addition to preservative is sequentially placed into stirring container, is mixed at room temperature to full and uniform；Preservative is added and continues stirring to sufficiently merging, takes upper layer free from admixture aliquots to obtain the Rhizoma Chuanxiong essential oil composition after centrifugal treating.Purposes is purposes of the above-mentioned Rhizoma Chuanxiong essential oil composition as skin-care product additive.Rhizoma Chuanxiong essential oil composition provided by the invention has whitening, inducing resuscitation, mosquito repellent and other effects, be it is a kind of it is easy to operate, raw material is natural and more effect essential oil products for having no toxic side effect.

Description

A kind of Rhizoma Chuanxiong essential oil composition and its preparation method and application
Technical field
The invention belongs to Chinese medicine health-care technical fields, more particularly, are related to a kind of with function such as whitening, inducing resuscitation, mosquito repellents Rhizoma Chuanxiong essential oil composition of effect and its preparation method and application.
Background technique
With the continuous improvement of people's living standards and the with rapid changepl. never-ending changes and improvements development of science and technology, the public demand to cosmetics Start to convert from past single nursing type to modern functions type.The effective component extracted in Chinese medicine usually has unique function With the advantages such as curative effect and Small side effects, become research hotspot in recent years as cosmetic additive agent.
In the prior art, mainly there are the kojic acid and arbutin inhibited tyrosinase activity, but its for the ingredient of whitening Stability is poor, has certain light sensitivity.In plant or Chinese herbal medicine extract for whitening substance have animal placenta extract, Licorice and endothelin antagonist etc. reach the nicotinoyl of whitening function there are also the melanocyte transfer for inhibiting melanin Amine and soybean etc..In the essential oil of plant extract, the ingredient with whitening effect mainly has Rosa Damascana, Lavender, lemon Substance made of one or more composition compatibilities such as lemon essential oil, tea tree ethereal oil, Geranium Essential, Peppermint essential oil, chrysanthemum essential oil.
Rhizoma Chuanxiong is that the dry rhizome of Umbelliferae rhizoma ligustici platymiscium Rhizoma Chuanxiong first recorded in Shennong's Herbal is common medium-height grass Medicine has activating microcirculation and removing stasis medicinal, promote breath and open depression, wind-expelling pain-stopping and other effects.Traditional Chinese medicine thinks that people's complexion is obscure, is since exopathogen is invaded It attacks, qi-blood-body fluid virtual loss, the deficiency of the kidney yin, liver lose that item reaches, the reasons such as the dysfunction of the spleen in transport cause；And facial colour spot is then due to liver depression blood The reasons such as the stasis of blood, spleen deficiency deficiency of the kidney, QI-blood circulation be unsmooth cause.Whitening should select removing blood stasis for promoting tissue regeneration, rich in nutrition, promote metabolism, promote The drug of blood, the i.e. Chinese medicine with activating microcirculation and removing stasis medicinal and the Chinese medicine for meeting cosmetics health specification.Modern pharmacological research is also sent out Existing, Rhizoma Chuanxiong has stronger inhibitory activity against tyrosinase, and has the function of stronger removing DPPH free radical, can be used as beauty The development object of white active material.
It is sold in essential oil and pertinent literature now, the rare whitening class product based on Rhizoma Chuanxiong.
Summary of the invention
In order to solve the problems in the existing technology, it is main that the object of the present invention is to provide one kind with rhizoma chuanxiong volatile oil Essential oil composition of ingredient and its preparation method and application.
An aspect of of the present present invention provides a kind of Rhizoma Chuanxiong essential oil composition, in parts by weight, the Rhizoma Chuanxiong essential oil composition Including following components: 45~55 parts of Rhizoma Chuanxiong essential oil, 43~50 parts of glycerol, 5~10 parts of peppermint oil and 0.05~0.1 part of preservative.
One embodiment of Rhizoma Chuanxiong essential oil composition according to the present invention, in terms of weight proportion, the Rhizoma Chuanxiong essential oil composition Including following components: 48~52 parts of Rhizoma Chuanxiong essential oil, 44~46 parts of glycerol, 7~8 parts of peppermint oil and 0.08~0.1 part of preservative.
One embodiment of Rhizoma Chuanxiong essential oil composition according to the present invention, in parts by weight, the Rhizoma Chuanxiong essential oil composition is also Including 5~10 parts of Lavender, 10~15 parts of Rosa Damascana and 5~10 parts of lemon.
One embodiment of Rhizoma Chuanxiong essential oil composition according to the present invention, the Rhizoma Chuanxiong essential oil use supercritical CO2Extraction It is obtained by extraction.
One embodiment of Rhizoma Chuanxiong essential oil composition according to the present invention, the supercritical CO2Extraction are as follows: by Ligusticum chuanxiong Hort Particle is packed into supercritical CO2It is extracted in extractor, the use of ethyl alcohol is entrainer, control extraction temperature is 50~60 DEG C, extracts Pressure power is 20~30MPa, and controlling the time extracted every time is 1.5~2.5h, and the Rhizoma Chuanxiong essential oil is finally obtained by extraction.
One embodiment of Rhizoma Chuanxiong essential oil composition according to the present invention, the Ligusticum chuanxiong Hort particle can cross 30~50 mesh medicines Allusion quotation sieve, the ethyl alcohol is the ethyl alcohol that volumetric concentration is 90~95%.
One embodiment of Rhizoma Chuanxiong essential oil composition according to the present invention, the preservative is by nepal Gold methyl ester, Nepal One of golden ethyl ester, Phenoxyethanol and methylisothiazolinone a variety of are mixed to get.
Another aspect provides the preparation methods of above-mentioned Rhizoma Chuanxiong essential oil composition, which is characterized in that the system Preparation Method the following steps are included:
Each component is weighed according to weight part ratio；
Each component in addition to preservative is sequentially placed into stirring container, is mixed at room temperature to full and uniform；
Preservative is added and continues stirring to sufficiently merging, takes upper layer free from admixture aliquots to obtain the river after centrifugal treating Rhizome of chuanxiong essential oil composition.
One embodiment of the preparation method of Rhizoma Chuanxiong essential oil composition according to the present invention, control centrifugal treating revolving speed are 3000 ~5000r/min, centrifugal treating time are 5~10min.
Another aspect of the invention provides purposes of the above-mentioned Rhizoma Chuanxiong essential oil composition as skin-care product additive.
The present invention provides one kind with rhizoma chuanxiong volatile oil Rhizoma Chuanxiong essential oil composition as main component and preparation method thereof and Purposes, be it is a kind of it is easy to operate, raw material is natural and more effect essential oil products for having no toxic side effect, additive therefor is less, can While playing skin care effect, the unnecessary injury to skin is reduced, there is effective whitening, inducing resuscitation and mosquito-repelling function, energy It is enough to be used alone or as additive.
Specific embodiment
All features disclosed in this specification or disclosed all methods or in the process the step of, in addition to mutually exclusive Feature and/or step other than, can combine in any way.
Any feature disclosed in this specification unless specifically stated can be equivalent or with similar purpose by other Alternative features are replaced.That is, unless specifically stated, each feature is an example in a series of equivalent or similar characteristics .
Rhizoma Chuanxiong essential oil composition of the present invention and its preparation method and application will be specifically described below.
Traditional Chinese medicine thinks that Rhizoma Chuanxiong has white-skinned face function, and ancient times have a record using Rhizoma Chuanxiong whitening, in history tree Also on the books.If " Prescriptions for Universal Relief " with Rhizoma Chuanxiong, each equal portions of cloves is smalls, wiping tooth can be as snow-white；" doctor's mirror at all times " is non-irrigated with Rhizoma Chuanxiong, ink Every early wiping tooth such as lotus, Radix Angelicae Sinensis, tooth soap, the root of Dahurain angelica, there is the benefits of permanent tooth, crow palpus；" General Records of Holy Universal Relief " uses Rhizoma Chuanxiong, the root of Dahurain angelica, Radix Glycyrrhizae, ramulus cinnamomi (removing rough bark), wild ginger etc. take daily, and body was all fragrant on 30th；" Guanji side " uses single Rhizoma Chuanxiong, and decocting contains it, fragrant mouth deodorization.From above Book on Chinese herbal medicine record as can be seen that Rhizoma Chuanxiong is in ancient times just for U.S. tooth, hairdressing, fragrant mouth perfume (or spice) body etc..Meanwhile Rhizoma Chuanxiong has relatively by force Inhibitory activity against tyrosinase and have the function of stronger removing DPPH free radical, in conjunction with tyrosinase inhibition test Result and remove DPPH free radical experiment result, it is believed that Rhizoma Chuanxiong can be used as the development object of whitening active substance.
Based on this, the present invention has developed one kind with rhizoma chuanxiong volatile oil Rhizoma Chuanxiong essential oil composition as main component, Neng Gouyou Effect realizes many-sided effect such as whitening, mosquito repellent, inducing resuscitation.
An exemplary embodiment of the present invention, in parts by weight, it is described for whitening, inducing resuscitation, mosquito repellent Rhizoma Chuanxiong essential oil Composition includes following components: 45~55 parts of Rhizoma Chuanxiong essential oil, 43~50 parts of glycerol, 5~10 parts of peppermint oil and preservative 0.05~ 0.1 part.
Wherein, Rhizoma Chuanxiong essential oil has the ability compared with high inhibition tyrosinase activity, free radical can be effectively removed, in whitening While skin, it is capable of the aging of delaying skin to a certain extent；On the other hand in the test of Rhizoma Chuanxiong essential oil, research is found Rhizoma Chuanxiong essential oil has certain mosquito-repelling function, compared to the chemical methodes mosquito repellent such as mosquito-repellent incense, insect aerosol is used, has nontoxic nothing The effect of public hazards, and can be reduced the pollution to environment.
The peppermint oil that the present invention is added has anti-inflammatory and antalgic, anti-bacteria and anti-virus, promotees infiltration, eliminating the phlegm, spasmolysis and inhibit maincenter Various pharmacological actions such as nervous system, be used in conjunction with Rhizoma Chuanxiong essential oil can be improved inducing resuscitation skin toner effect while, promote river The infiltration of rhizome of chuanxiong essential oil.And glycerol has the function of moisturizing, water lock, may advantageously facilitate the absorption of skin and keep skin moisture and Gloss.
Preferred embodiment in accordance with the present invention, in parts by weight, the Rhizoma Chuanxiong essential oil composition includes following components: Rhizoma Chuanxiong 48~52 parts of essential oil, 44~46 parts of glycerol, 7~8 parts of peppermint oil and 0.08~0.1 part of preservative.
In addition, in order to increase the white-skinned face function of Rhizoma Chuanxiong essential oil composition and improve fragrance, the present invention can also add Rhizoma Atractylodis Macrocephalae, The extraction of essential oil object and Rosa Damascana of Radix Astragali Chinese medicine, lemon and Lavender etc., being formed has unique perfume and function The Rhizoma Chuanxiong essential oil composition of effect.
Specifically, one embodiment of Rhizoma Chuanxiong essential oil composition, the Rhizoma Chuanxiong essential oil composition further include according to the present invention 5~10 parts of Lavender, 10~15 parts of Rosa Damascana and 5~10 parts of lemon.
According to the present invention, the Rhizoma Chuanxiong essential oil self-control used in the present invention obtains, also can be by commercially available.This hair The bright self-control Rhizoma Chuanxiong essential oil preferably uses supercritical CO2Extraction is obtained by extraction.Specifically, Ligusticum chuanxiong Hort particle is filled Enter supercritical CO2It is extracted in extractor, the use of ethyl alcohol is entrainer, control extraction temperature is 50~60 DEG C, extracting pressure For 20~30MPa, wherein the time that control extracts every time is 1.5~2.5h, and Rhizoma Chuanxiong essential oil is finally obtained by extraction.Wherein, above-mentioned Ligusticum chuanxiong Hort particle can cross 30~50 mesh pharmacopeia sieve, and used ethyl alcohol is the ethyl alcohol that volumetric concentration is 90~95%.
Peppermint oil of the present invention, Rosa Damascana, lemon, glycerol etc. are commercially available, wherein peppermint oil, rose The essential oils such as rare essential oil meet standard GB/T/T26516-2011 essential oil quality criteria requirements, and glycerol meets national standard Q/HYH D34-2008 requirement.In addition, Rhizoma Atractylodis Macrocephalae essential oil, root of Dahurain angelica essential oil, Radix Astragali essential oil can equally take it is similar with Rhizoma Chuanxiong extraction of essential oil Method is extracted, and commercial product can also be bought.
Preservative of the present invention is by nepal Gold methyl ester, Nepal's gold ethyl ester, Phenoxyethanol and methyl-isothiazol One of quinoline ketone a variety of is mixed to get.
Invention also provides the preparation methods of above-mentioned Rhizoma Chuanxiong essential oil composition.Exemplary implementation according to the present invention Example, the preparation method comprises the following steps:
Each component is weighed according to weight part ratio；
Each component in addition to preservative is sequentially placed into stirring container, is mixed at room temperature to full and uniform；
Preservative is added and continues stirring to sufficiently merging, takes upper layer free from admixture aliquots to obtain the river after centrifugal treating Rhizome of chuanxiong essential oil composition.
Wherein it is preferred to which controlling centrifugal treating revolving speed is 3000~5000r/min, the centrifugal treating time is 5~10min.
Above-mentioned Rhizoma Chuanxiong essential oil composition has the purposes as skin-care product additive.That is, according to the difference of usage, this The Rhizoma Chuanxiong essential oil composition of invention can be used as additive and be added in skin care item, also can be used alone.Due to the Rhizoma Chuanxiong essential oil The preparation method of composition is easy to operate, and additive therefor is less, while playing skin care effect, can reduce to skin Unnecessary injury.
It should be understood that above embodiment and following embodiment that the present invention is described in detail are merely to illustrate the present invention rather than limit The scope of the present invention processed, some nonessential improvement and tune that those skilled in the art's above content according to the present invention is made It is whole to all belong to the scope of protection of the present invention.
Rhizoma Chuanxiong essential oil composition of the present invention and its preparation method and application is made furtherly combined with specific embodiments below It is bright.
Embodiment 1-3: the preparation of Rhizoma Chuanxiong essential oil composition
Listed correspondence weight weighs each component raw material according to the form below 1.
Proportion (the unit: part of Rhizoma Chuanxiong essential oil composition in 1 embodiment 1-3 of table；Wherein, every part is 10g)
Embodiment 1 Embodiment 2 Embodiment 3
Rhizoma Chuanxiong essential oil 52 48 50
Peppermint oil 8 6 7
Glycerol 45 44 46
Phenoxyethanol 0.1 / 0.05
Nepal Gold methyl ester / 0.08 0.05
Wherein, Rhizoma Chuanxiong essential oil is to make by oneself and use supercritical CO2It is obtained by extraction, method particularly includes: by Ligusticum chuanxiong Hort Grain (crossing 30 mesh pharmacopeia sieve) is packed into supercritical CO2In extractor, control extraction temperature be 50 DEG C, extracting pressure 25MPa, control Each extraction time is 2h, and using volumetric concentration for 95% ethyl alcohol is entrainer；Peppermint oil be it is commercially available, meet national standard GB/T26516-2011 essential oil quality criteria requirements；Glycerol be it is commercially available, meet national standard Q/HYH D34-2008 requirement.
The preparation method of above-described embodiment 1-3 is equal are as follows:
1) each component is weighed according to weight listed by table 1；
2) Rhizoma Chuanxiong essential oil, peppermint oil, glycerol are sequentially placed into stirring container, are mixed at room temperature to full and uniform；
3) preservative is added and continues stirring to sufficiently merging, after centrifugal treating (4000r/min) about 5min, takes upper layer without miscellaneous Matter aliquots are up to Rhizoma Chuanxiong essential oil composition.
Embodiment 4-5: improve the Rhizoma Chuanxiong essential oil composition of essential oil odor type
Demand for further satisfaction crowd to essential oil whitening, the present invention are adjusted on the basis of original formula, are fitted Answer different crowd to essential oil odor type requirement, listed correspondence weight weighs each component raw material in specific according to the form below 2.
Proportion (the unit: part of Rhizoma Chuanxiong essential oil composition in 2 embodiment 4-5 of table；Wherein, every part is 10g)
Embodiment 4 Embodiment 5
Rhizoma Chuanxiong essential oil 49 45
Lavender 10 8
Lemon 8 7
Rosa Damascana 10 15
Peppermint oil 5 5
Glycerol 43 44
Phenoxyethanol 0.1 /
Nepal Gold methyl ester / 0.08
Lavender, lemon, Rosa Damascana be it is commercially available, meet standard GB/T/T26516-2011 essential oil matter Measure standard requirements；Glycerol be it is commercially available, meet national standard Q/HYH D34-2008 requirement.
The preparation method is the same as that of Example 1-3.
Embodiment 6: using Rhizoma Chuanxiong essential oil composition made from embodiment 1 as the system of skin-care product additive and its gel mask It is standby.
It is formulated as follows: 0.5 part of xanthan gum, 0.5 part of locust bean gum, 0.3 part of monoglyceride, 0.03 part of EDTA, Phenoxyethanol 0.1 Part, 2 parts of propylene glycol, 0.5 part of sodium hyaluronate, 2 parts of sodium benzoate, 0.5 part of Rhizoma Chuanxiong essential oil composition, 70 parts of distilled water.
Preparation method: the dry feedstocks such as sodium hyaluronate, sodium benzoate, xanthan gum, locust bean gum are weighed respectively by weight ratio Component is uniformly mixed, obtains A phase；Propylene glycol, monoglyceride, distilled water are weighed by weight ratio, and stirred evenly, B is obtained Phase；The B phase being poured slowly into lasting stirring into A phase powder, is heated at a temperature of 60~80 DEG C, continues to stir to completely molten Solution；After object to be mixed is cooling, Rhizoma Chuanxiong essential oil composition is added while stirring, until fusion is uniform completely, and is put into centrifuge In, after 4000r/min handles 3min, obtain gel mask.
Embodiment 7: Rhizoma Chuanxiong essential oil composition inhibits tyrosinase activity test
1) measuring principle
L-tyrosine generates DOPA under the action of tyrosinase, and DOPA generates DOPA quinone under the further effect of enzyme, Finally melanin is generated by series reaction.Wherein DOPA quinone has maximum absorption band at 475nm wavelength, passes through the unit time It is interior, its absorbance is measured, the vigor size of enzyme in indirect reaction enzymic catalytic reaction system.DOPA quinone i.e. per minute is in 475nm wave Long absorbance increases by 0.001 and is defined as an enzyme activity unit.
2) preparation of solution
The preparation of the solution of phosphate-buffered salt: Na is weighed2HPO4·12H2O 8.775g, NaH2PO4·2H2O3.98g is used Deionized water sufficiently dissolves constant volume in 250mL volumetric flask, is configured to the phosphate buffer for the 0.2mol/L that pH value is 6.8.
The preparation of tyrosinase solution: it is that 200U/mL weighs a certain amount of tyrosinase according to enzyme activity unit, uses phosphoric acid Salt buffer dissolution, constant volume are stored in spare in refrigerator in the volumetric flask of required volume.
The preparation of l-tyrosine solution: precision weighs l-tyrosine 0.0272g, pre-dispersed with phosphate buffer, ultrasound 30min, with phosphate buffer constant volume in 100mL volumetric flask.
3) measuring method
The corresponding l-tyrosine solution of T1, T2, T3, T4, PBS are accurately drawn by the volume of table 4 respectively with micropipettor And the reaction solution of sample is respectively placed in 4 PE pipes, is mixed, then 37 DEG C of constant temperature 10min are separately added into 1mL's in T2, T4 Tyrosinase solution, react 10min, quickly with ultraviolet specrophotometer measured at 475nm its absorbance A T1, AT2, AT3, AT4.The activity that sample inhibits tyrosinase is calculated as follows:
Inhibitory activity against tyrosinase=[1- (AT4-AT3)/(AT2-AT1)] × 100%.
Wherein, AT1: the absorbance for not being loaded product and the reaction solution of tyrosinase solution not being added to measure at 475nm；
AT2: the absorbance that the product of not being loaded do not add the reaction solution of tyrosinase solution to measure at 475nm；
AT3: sample-adding product and not plus the absorbance that is measured at 475nm of reaction solution of tyrosinase solution；
AT4: sample-adding product and the absorbance for adding the reaction solution of tyrosinase solution to measure at 475nm.
Wherein, the Rhizoma Chuanxiong essential oil composition that the present embodiment sample uses embodiment 3 to be prepared, control group, which uses, has beauty The Rosa Damascana (commercially available, to meet standard GB/T/T26516-2011 essential oil quality criteria requirements) of white effect.
3 Rhizoma Chuanxiong essential oil composition of table inhibits tyrosinase activity test reaction solution composition
4) measurement result
According to test result, it is measured in parallel the inhibitory activity against tyrosinase of 2 Rhizoma Chuanxiong essential oil compositions, respectively 33.52%, 34.21%, reference substance Rosa Damascana inhibitory activity against tyrosinase is only 21.35%, illustrates Rhizoma Chuanxiong of the invention Essential oil composition has significantly higher tyrosinase inhibition rate, shows that Rhizoma Chuanxiong essential oil composition can be by inhibiting tyrosinase Activity, the effect of reducing the formation of melanin, and reach whitening.
Embodiment 8: Rhizoma Chuanxiong essential oil composition mosquito repellent test
The present embodiment invites 60 testing crews, and Yu Qiyue mosquito activity more dense days carry out repelling effect tune on probation It looks into.
Application method: the drop drop of Rhizoma Chuanxiong essential oil composition 1~2 that embodiment 1 is prepared uniformly is applied on skin Easily by the position of bite by mosquitos.Using follow-up investigation after 1 month, it the results are shown in Table 4.As can be known from the results, Rhizoma Chuanxiong essential oil composition has There is certain repelling effect.
The repelling effect of 4 Rhizoma Chuanxiong essential oil composition of table is investigated
Remarks:
Repelling effect is good: after smearing, nothing stings thorn phenomenon；
Repelling effect is general: after smearing, mosquito quantity is significantly reduced, and mosquito stings thorn number and reduces；
Without repelling effect: after smearing, mosquito quantity stings thorn phenomenon without change.
Embodiment 9: the whitening of Rhizoma Chuanxiong essential oil composition, inducing resuscitation effect test
The smell of Rhizoma Chuanxiong essential oil is mainly adjusted with Rhizoma Chuanxiong whitening, peppermint oil inducing resuscitation skin toner in Rhizoma Chuanxiong essential oil composition.To test The 2 effect effects of whitening, inducing resuscitation for demonstrate,proving Rhizoma Chuanxiong essential oil composition, invite several health volunteers to participate in the test of the present embodiment, Age 22~56 years old, more black or obscure women 50 of the colour of skin.It is carried out using the Rhizoma Chuanxiong essential oil composition that embodiment 1 is prepared Test, daily each 1 time of morning and evening.Application method: it after clean skin, by the drop of Rhizoma Chuanxiong essential oil composition 2~4 in the palm of the hand, massages in face extremely It absorbs.
Using subject's test result is investigated after 1 month, concrete outcome is shown in Table 5.The result shows that Rhizoma Chuanxiong essential oil composition has There are certain whitening, inducing resuscitation, and does not find apparent irritation and allergic phenomena after trying out.
The whitening of 5 Rhizoma Chuanxiong essential oil composition of table, inducing resuscitation test result
In conclusion the present invention provides one kind with rhizoma chuanxiong volatile oil Rhizoma Chuanxiong essential oil composition as main component and its system Preparation Method and purposes, be it is a kind of it is easy to operate, raw material is natural and more effect essential oil products for having no toxic side effect, additive therefor compared with It is few, the unnecessary injury to skin can be reduced while playing skin care effect, there is effective whitening, inducing resuscitation and mosquito repellent Effect, can use alone or as additive, have Development volue, can enrich essential oil product market, meet consumer Demand.
The invention is not limited to specific embodiments above-mentioned.The present invention, which expands to, any in the present specification to be disclosed New feature or any new combination, and disclose any new method or process the step of or any new combination.

Claims (10)

1. a kind of Rhizoma Chuanxiong essential oil composition, which is characterized in that in parts by weight, the Rhizoma Chuanxiong essential oil composition is by following components group At: 45~55 parts of Rhizoma Chuanxiong essential oil, 43~50 parts of glycerol, 5~10 parts of peppermint oil and 0.05~0.1 part of preservative, wherein the river Rhizome of chuanxiong essential oil uses supercritical CO2Extraction is obtained by extraction, the supercritical CO2Extraction are as follows: Ligusticum chuanxiong Hort particle is packed into super face Boundary CO2Extracted in extractor, the use of ethyl alcohol be entrainer, control extraction temperature is 50~60 DEG C, extracting pressure be 20~ 30MPa, controlling the time extracted every time is 1.5~2.5h, and the Rhizoma Chuanxiong essential oil is finally obtained by extraction.
2. Rhizoma Chuanxiong essential oil composition according to claim 1, which is characterized in that in parts by weight, the Rhizoma Chuanxiong essential oil group It is composed of the following components to close object: 48~52 parts of Rhizoma Chuanxiong essential oil, 44~46 parts of glycerol, 7~8 parts of peppermint oil and preservative 0.08~ 0.1 part.
3. Rhizoma Chuanxiong essential oil composition according to claim 1, which is characterized in that the Ligusticum chuanxiong Hort particle can cross 30~ 50 mesh pharmacopeia sieve, the ethyl alcohol is the ethyl alcohol that volumetric concentration is 90~95%.
4. Rhizoma Chuanxiong essential oil composition according to claim 1, which is characterized in that the preservative by nepal Gold methyl ester, One of Nepal's gold ethyl ester, Phenoxyethanol and methylisothiazolinone a variety of are mixed with to obtain.
5. a kind of Rhizoma Chuanxiong essential oil composition, which is characterized in that in parts by weight, the Rhizoma Chuanxiong essential oil composition is by following components group At: 45~55 parts of Rhizoma Chuanxiong essential oil, 43~50 parts of glycerol, 5~10 parts of peppermint oil and 0.05~0.1 part of preservative, Lavender 5 ~10 parts, 10~15 parts of Rosa Damascana and 5~10 parts of lemon, wherein the Rhizoma Chuanxiong essential oil uses supercritical CO2Extraction It is obtained by extraction, the supercritical CO2Extraction are as follows: Ligusticum chuanxiong Hort particle is packed into supercritical CO2It is extracted, is made in extractor It is entrainer with ethyl alcohol, control extraction temperature is 50~60 DEG C, extracting pressure is 20~30MPa, controls the time extracted every time For 1.5~2.5h, the Rhizoma Chuanxiong essential oil is finally obtained by extraction.
6. Rhizoma Chuanxiong essential oil composition according to claim 5, which is characterized in that the Ligusticum chuanxiong Hort particle can cross 30~ 50 mesh pharmacopeia sieve, the ethyl alcohol is the ethyl alcohol that volumetric concentration is 90~95%.
7. Rhizoma Chuanxiong essential oil composition according to claim 5, which is characterized in that the preservative by nepal Gold methyl ester, One of Nepal's gold ethyl ester, Phenoxyethanol and methylisothiazolinone a variety of are mixed with to obtain.
8. the preparation method of Rhizoma Chuanxiong essential oil composition as described in any one of claims 1 to 7, which is characterized in that the preparation Method the following steps are included:
Each component is weighed according to weight part ratio；
Each component in addition to preservative is sequentially placed into stirring container, is mixed at room temperature to full and uniform；
Preservative is added and continues stirring to sufficiently merging, upper layer free from admixture aliquots is taken to obtain the Rhizoma Chuanxiong essence after centrifugal treating Fluid composition.
9. using the preparation method of Rhizoma Chuanxiong essential oil composition according to claim 8, which is characterized in that control centrifugal treating revolving speed For 3000~5000r/min, the centrifugal treating time is 5~10min.
10. purposes of the Rhizoma Chuanxiong essential oil composition as skin-care product additive as described in any one of claims 1 to 7.
CN201710070862.5A 2017-02-09 2017-02-09 A kind of Rhizoma Chuanxiong essential oil composition and its preparation method and application Active CN106821803B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710070862.5A CN106821803B (en) 2017-02-09 2017-02-09 A kind of Rhizoma Chuanxiong essential oil composition and its preparation method and application

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201710070862.5A CN106821803B (en) 2017-02-09 2017-02-09 A kind of Rhizoma Chuanxiong essential oil composition and its preparation method and application

Publications (2)

Publication Number Publication Date
CN106821803A CN106821803A (en) 2017-06-13
CN106821803B true CN106821803B (en) 2019-11-29

Family

ID=59122171

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201710070862.5A Active CN106821803B (en) 2017-02-09 2017-02-09 A kind of Rhizoma Chuanxiong essential oil composition and its preparation method and application

Country Status (1)

Country Link
CN (1) CN106821803B (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110169932A (en) * 2019-06-27 2019-08-27 东莞怀朴植物精油有限公司 A kind of nti-freckle compound essential oil and preparation method thereof
CN111481489A (en) * 2020-05-20 2020-08-04 四川绿色本草科技发展有限公司 Ligusticum wallichii extract with mosquito repelling and itching relieving effects and application thereof
CN113214904A (en) * 2021-06-17 2021-08-06 五邑大学 Preparation method of plant essential oil with tyrosinase activity inhibition function
CN115040439B (en) * 2022-06-30 2024-03-01 成都中医药大学 Ligusticum wallichii essential oil skin care product and preparation method thereof

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100504408B1 (en) * 2002-10-29 2005-07-27 주식회사 코리아나화장품 Cosmetic Composition for Skin-Whitening Comprising Senkyunolide A as Active Ingredient
CN104997702B (en) * 2015-08-18 2018-02-16 云南同方科技有限公司 A kind of formula for dispelling pox essential oil
CN105878059A (en) * 2016-06-28 2016-08-24 福建万亿店中店电子商务有限责任公司 Tea compound essential oil with weight-loss effect

Also Published As

Publication number Publication date
CN106821803A (en) 2017-06-13

Similar Documents

Publication Publication Date Title
CN106821803B (en) A kind of Rhizoma Chuanxiong essential oil composition and its preparation method and application
CN102641228B (en) Rhodiola crenulata eye cream with whitening and anti-wrinkle effects
KR100822835B1 (en) Plant extracts and cosmetic compositions for improvement of scalp and hair
CN101884608B (en) Mosquito repelling composition
Potluri et al. A review on herbs used in anti-dandruff shampoo and its evaluation parameters
CN103565730A (en) Scented tea whitening skin-moistening cream and preparation method thereof
CN106236624A (en) A kind of hydrogel facial film with whitening effect and preparation method thereof
CN104784507A (en) Traditional Chinese medicine composition with multiple cosmetic effects and application
CN109661237A (en) It is set as the skin irritatin as caused by micronic dust and scytitis alleviation of effective component with composition comprising holy basil, peppermint, margosa tree tree extraction mixture
CN104224683A (en) Natural plant skin care product for improving skin metabolism of yellow race and delaying senescence
CN107375095A (en) A kind of whitening, moisturizing essence and preparation method thereof
KR100564108B1 (en) Cosmetic composition comprising plant extract having anti-aging effect
CN106177100A (en) A kind of compositions with anti-acne effect and application thereof
CN108114074A (en) Breast detumescence relieves the pain rhizome of chuanxiong fresh medicine extraction composition, fresh medicine oil and preparation method thereof
CN110448505A (en) A kind of preparation method and products thereof tearing herb powder facial mask
CN109381419A (en) Cosmetic composition comprising silver-colored sweet-scented osmanthus extract and cognate clematis extract as effective component
CN101695470B (en) Composite for cosmetics, cosmetic product containing same and preparation method thereof
CN106726993A (en) Chinese medicine hair cream and preparation method
CN109431951A (en) A kind of Chinese medicine lightening compositions and preparation method thereof
CN105326713A (en) Toner and preparation method thereof
CN108703911A (en) A kind of composition and its preparation method and application with moisturizing white-skinned face function
CN105267082A (en) Impatiens balsamina extract and application thereof
KR20170137462A (en) Composition for improving skin condition comprising herb extracts mixture
JP3923424B2 (en) Cosmetics for hair removal
JP4223170B2 (en) Hair composition

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant