CN106795651B - 利用单管添加方案的加标签的核酸的文库制备 - Google Patents

利用单管添加方案的加标签的核酸的文库制备 Download PDF

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CN106795651B
CN106795651B CN201580046283.6A CN201580046283A CN106795651B CN 106795651 B CN106795651 B CN 106795651B CN 201580046283 A CN201580046283 A CN 201580046283A CN 106795651 B CN106795651 B CN 106795651B
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nucleic acid
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tagged
sequencing
proteases
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CN106795651A (zh
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F·凯珀
戈登·卡恩
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Illumina Inc
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor

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CN201580046283.6A 2014-06-26 2015-06-25 利用单管添加方案的加标签的核酸的文库制备 Active CN106795651B (zh)

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US201462027198P 2014-07-21 2014-07-21
US62/027,198 2014-07-21
PCT/US2015/037653 WO2015200609A1 (en) 2014-06-26 2015-06-25 Library preparation of tagged nucleic acid using single tube add-on protocol

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