CN106726671A - Essence comprising recombinant human metallothionein tunning extract and its application in cosmetics - Google Patents
Essence comprising recombinant human metallothionein tunning extract and its application in cosmetics Download PDFInfo
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- CN106726671A CN106726671A CN201611163780.7A CN201611163780A CN106726671A CN 106726671 A CN106726671 A CN 106726671A CN 201611163780 A CN201611163780 A CN 201611163780A CN 106726671 A CN106726671 A CN 106726671A
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- recombinant human
- human metallothionein
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/44—Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/04—Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Dermatology (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Cosmetics (AREA)
Abstract
The present invention relates to composition and its different dosage forms with recombinant human metallothionein tunning extract as core active composition, it is characterised in that comprising recombinant human metallothionein tunning extract, the lyophilized formulations that oligopeptides 1, Trenaxmine is main component, solution and liquid crystal type essence.The amino acid sequence of wherein described recombinant human metallothionein is SEQ ID No.1, and the amino acid sequence of oligopeptides 1 is SEQ ID No.2.The essence have subtract wrinkle brighten, the effect of the tender skin of moisturizing and ultraviolet radiation preventing, supplemented while skin free radical the trace element such as Zn, Fe, Cu to adjust local skin microcirculation, promote epidermis reparation and healing by removing, and then reach the purpose of skin whitening.Further, since metallothionein is signed an undertaking closes the specific function of heavy metal, this essence has special effect to the cosmetic crowd for causing the skin damages such as color spot, wrinkle by the pigment of cosmetic containing heavy metal.
Description
Technical field
The invention belongs to cosmetic field.In particular it relates to a kind of extract comprising metallothionein tunning
The essence and its cosmetic composition of thing, the essence have subtract wrinkle brighten, the tender skin of moisturizing and ultraviolet radiation preventing
Effect, further, since metallothionein is signed an undertaking closes the specific function of heavy metal, this essence is to by the pigment of cosmetic containing heavy metal
Causing the cosmetic crowd of the skin damages such as color spot, wrinkle has special effect.
Background technology
With age, the aspect such as body tissue organ and cell function structure occur a series of chronic, progressive and
Degeneration changes, and these changes bring adverse effect to body, cause its adaptability and reserve capabillity to decline increasingly, this change
The continuous occurrence and development of change process are aging.
The distinguishing mark of aging is skin senescence, and its typical performance is exactly that the drying of senile plaque expelling itch, coarse or dermatitis etc. are asked
The appearance of topic.Research finds that the presence of machine interior free yl can promote to produce melanin, lipofuscin and ceroid in skin histology
Deng aging material.This reaction and its product will promote skin darkening, tough and tensile collagen, contraction in length, expansive force to weaken, Jin Erzao
Into the skin symptom such as seabuckthorn oil, wrinkle, color spot.In human normal physiology course, body can secret out of a series of use
Carry out the material of adjustment intracellular free radicals concentration to ensure the vigorous and graceful and youth of skin.But with age, disease, wound
The influence of the environmental factor such as wound and ultraviolet irradiation, contact chemical substance, the physiological function decline of organism balance number of free radical,
Free radical produces ability enhancing, causes skin health and the situation of youth to be destroyed, and then freckle, itch occurs in skin histology
Dry, coarse or dermatitis etc. changes.These changes not only have impact on attractive in appearance, also be unfavorable for the performance of skin histology function.
On the other hand, with the improvement of people ' s living standards, people also begin to after basic clothing, food, lodging and transportion -- basic necessities of life demand is met
Pay close attention to other aspects especially body, the demand of the aspect such as attractive in appearance of appearance.And in order to pursue the attractive in appearance of appearance, mascara, eye
The cosmetics such as shadow, lipstick have turned into daily " the necessary class " of many modern females.Most of cosmetics only various chemistry
The synthetic of material, this kind of chemicals synthetic of long-term use or cosmetics inferior can also cause wrinkle of skin, color spot, inflammation etc.
Problems.What is more, skin and internal Heavy Metal Accumulation occurs, and then serious harm is healthy.
Metallothionein is nineteen fifty-seven Margoshes and Vallee when metal biological action is studied, from animal organ
Isolate a kind of new protein.Research finds metallothionein because its molecular weight is smaller, it is easy to is absorbed by the skin and is impregnated with
To in histocyte, the physiological activity of intracellular removing free radical can be directly participated in, play the work of rapid regulation free radical balance
With, the problems such as so as to inhibit the generation of melanin, lipofuscin, ceroid etc., and then slow down wrinkle of skin, color spot and inflammation
Occur.On the other hand, because metallothionein can remove the unnecessary free radical produced because sunlight irradiates in skin histology, therefore
The albumen also has the ability of certain ionizing radiation-resistant.Further, since sulfydryl of the metallothionein rich in cysteine can be combined
Metal ion, and the process is reversible.When internal certain metal ion content is excessive, metallothionein can combine them simultaneously
Excreting makes its content tend to normal；Otherwise can be discharged again, the scarce element of institute in added body, the internal micro unit of regulation
The balance of element, plays a part of a kind of Physiological effect device.
Magical effect of metallothionein causes the attention of numerous cosmetologists, and they attempt and start as biology
Type additive is used in cosmetics, such as MT serial cosmetics that great Bao Cosmetics Plants are released etc..But presently commercially available metallothionein
White to be mostly using animal productiong and heavy metal induction gained, yield is extremely low and purification step is complicated.Thus metallothionein is caused
It is white expensive, substantially increase the production cost of such cosmetics.Additionally, research finds to add gold in food or cosmetics
Category its anti-oxidant, anti-aging of sulfoprotein fermentation broth coarse extract and radioresistance effect are substantially better than pure protein, therefore product of the present invention makes
Metallothionein is including but not limited to obtaining (referring to patent containing metallothionein by gene recombination technology expression：
The recombinant expression carrier and method CN 101144093A of soluble expressing human I type metallothioneins) tunning extract,
Efficiently solve the problems such as the low, somewhat expensive of the protein yield.
The content of the invention
In view of this, it is an object of the invention to provide a kind of comprising recombinant human metallothionein tunning extract
Essence and combinations thereof.The essence and composition specifically include recombinant human metallothionein tunning extract, widow
Peptide -1, Trenaxmine etc..The essence is selected rich in cysteine residues and has compatibility etc. multiple to many kinds of metal ions
The recombinant human metallothionein tunning extract of unique effects, by adjusting skin microelement concentration, removing free radical
And then improve local skin microcirculation, the differentiation of stimulation epidermal cell and correction, promote epidermis reparation and healing, recover skin work
Power, so reach the tender skin of moisturizing, subtract wrinkle brighten and ultraviolet radiation preventing effect.
Further, since metallothionein is signed an undertaking closes the specific function of heavy metal, this essence is particularly suited for by containing weight
Metal cosmetic pigment causes the cosmetic crowd of the skin damages such as color spot, wrinkle.This essence can be further prepared into aqua, breast
The forms such as agent, gel, cream, for the exploitation of anti-ageing maintenance series of products.
To achieve the above object, the first aspect of the present invention provides one kind and is carried comprising recombinant human metallothionein tunning
The essence of thing is taken, it includes the recombinant human metallothionein tunning extract of 0.5-30 weight %, 0.001-2 weight %
Oligopeptides -1, the Trenaxmine of 0.01-5 weight %, the hyaluronic acid of 1-10 weight %, the palpus extractive of usnea of 0.5-3 weight %,
The long and thin hot pepper berry extract of 0.5-3 weight %, balance of water, wherein the recombinant human metallothionein such as SEQ ID No：Shown in 1,
The oligopeptides -1 such as SEQ ID No：Shown in 2.
Preferably, wherein the recombinant human metallothionein tunning extract is including but not limited to following one kind
Or it is various：Saccharomycetes to make fermentation extract of the recombinant human metallothionein through recombinantly expressing, Escherichia coli fermentation extract or lactic acid
Bacterium extractive from fermentative.
Preferably for the recombinant human metallothionein tunning extract, contained destination protein in its zymotic fluid
(that is, SEQ ID No：1) the 20-25 weight % of zymotic fluid are accounted for, final " the fermentation product comprising recombinant human metallothionein for extracting
Destination protein comprising 20-25 weight % in thing extract ".It should be appreciated by those skilled in the art that common molecular can be utilized
Cloning process will be comprising SEQ ID No：The recombinant expression carrier of the coded sequence of the recombinant human metallothionein shown in 1 is cloned into
In appropriate Host Strains (such as, but not limited to, Escherichia coli, saccharomycete, lactic acid bacteria etc.), express under suitable condition, profit
Acquisition " recombinant human metallothionein tunning extract " is slightly proposed with general extraction methods, as long as that finally extracts " includes weight
The destination protein comprising 20-25 weight % is that can be used for mesh of the invention in the tunning extract of group human metal thioalbumen "
's.
Wherein, the recombination expression and purification process of the recombinant human metallothionein can be found in CN 101144093A (inventions
Entitled " recombinant expression carrier and method of soluble expressing human I type metallothioneins ", date of application：On September 13rd, 2006,
Application number：200610112960.2, publication date：On March 19th, 2008).The amino acid sequence of recombinant human metallothionein is：
Met Asp Pro Asn Cys Ser Cys Ala Thr Gly Gly Ser Cys Thr Cys Thr Gly Ser Cys
Lys Cys Lys Glu Cys Lys Cys Asn Ser Cys Lys Lys Ser Cys Cys Ser Cys Cys Pro
Met Ser Cys Ala Lys Cys Ala Gln Gly Cys Ile Cys Lys Gly Ala Ser Glu Lys Cys
Ser Cys Cys Ala(SEQ ID No：1).
Wherein, the oligopeptides -1 is that, through gene recombination technology gained, its amino acid sequence is：Asn Ser Asp Ser
Glu Cys Pro Leu Ser His Asp Gly Tyr Cys Leu His Asp Gly Val Cys MET Tyr Ile
Glu Ala Leu Asp Lys Tyr Ala Cys Asn Cys Val Val Gly Tyr Ile Gly Glu Arg Cys
Gln Tyr Arg Asp Leu Lys Trp Trp Glu Leu Arg(SEQ ID No：2).
The second aspect of the present invention provides a kind of preparation method of recombinant human metallothionein yeast tunning extract,
Methods described is comprised the steps of：
Step one：The recombinant expression carrier of metallothionein is built, its construction method can be found in CN 101144093A (hairs
Bright entitled " recombinant expression carrier and method of soluble expressing human I type metallothioneins ", date of application：September 13 in 2006
Day, application number：200610112960.2, publication date：On March 19th, 2008)；
Step 2：The recombinant expression carrier built in step one is converted into host cell, and is inoculated in 50L fermentation tanks
In, fermentation temperature is 27 DEG C, and rotating speed is 150rpm/min, and throughput is controlled in 1.67vvm, fermentation time 48h；
Step 3：Zymotic fluid takes supernatant Rotary Evaporators with 6000rpm/min centrifugation 10min after the completion of fermenting
The 20-25 weight % that contained destination protein in zymotic fluid accounts for zymotic fluid is concentrated under reduced pressure at 45 DEG C.
Host cell wherein used in step 2 can be selected from yeast cells, lactic acid bacteria or Escherichia coli.
It should be appreciated by those skilled in the art that available host cell is not limited to above-mentioned cell.For " comprising recombined human gold
The zymotic fluid of category sulfoprotein tunning extract ", it is also possible to extracted to zymotic fluid with various methods, as long as in zymotic fluid
Contained destination protein (that is, SEQ ID No：1) the 20-25 weight % for accounting for zymotic fluid can use routine protein extracting method to be carried
Take, the destination protein of 20-25 weight % is included in final " the tunning extract comprising recombinant human metallothionein " (i.e.,
SEQ ID No：1).
The third aspect of the present invention provides a kind of liquid crystal type essence comprising recombinant human metallothionein tunning extract
Hua Su, each component comprising following percentage by weight：The tunning of the recombinant human metallothionein of 0.5-30 weight % is extracted
Thing, the oligopeptides -1 of 0.001-2 weight %, the Trenaxmine of 0.01-5 weight %, the D-pantothenyl aleohol of 1-6 weight %, the ammonia of 1-5 weight %
Base acid NMF, the glycerine of 1-10 weight %, the palpus extractive of usnea of 0.5-3 weight %, the prickly ash berries of 0.5-3 weight % are extracted
Thing, the hexadecanol of 1-8 weight %, the octadecyl alcolol of 1-8 weight %, the glycerin monostearate of 1-6 weight %, 1-6 weight %'s
16 octadecyl glucosides, the white oil of 1-10 weight %, the sad certain herbaceous plants with big flowers acid glyceride of 0.5-5 weight %, balance of water, its
Described in recombinant human metallothionein such as SEQ ID No：Shown in 1, the oligopeptides -1 such as SEQ ID No：Shown in 2.
In a preferred embodiment in accordance with this invention, it is described comprising recombinant human metallothionein tunning extract
Liquid crystal type essence, each component comprising following percentage by weight：The restructuring metallothionein tunning of 20 weight % is carried
Take thing, the oligopeptides -1 of 0.1 weight %, the Trenaxmine of 1 weight %, the D-pantothenyl aleohol of 2 weight %, the amino acid humectant of 3 weight %,
The glycerine of 5 weight %, the palpus extractive of usnea of 1 weight %, the long and thin hot pepper berry extract of 1 weight %, the hexadecanol of 3 weight %, 3 weights
The octadecyl alcolol of amount %, the glycerin monostearate of 2 weight %, the 16 octadecyl glucosides of 2 weight %, 5 weight %'s
White oil, the sad certain herbaceous plants with big flowers acid glyceride of 2 weight %, balance of water, wherein the recombinant human metallothionein such as SEQ ID No：1 institute
Show, the oligopeptides -1 such as SEQ ID No：Shown in 2.
It is described to be extracted comprising recombinant human metallothionein tunning in another preferred embodiment of the present invention
The liquid crystal type essence of thing, specifically comprising each component of following percentage by weight：The recombinant human metallothionein hair of 25 weight %
Ferment product extract, the oligopeptides -1 of 0.05 weight %, the Trenaxmine of 0.5 weight %, the D-pantothenyl aleohol of 2.5 weight %, 2.5 weight %
Amino acid humectant, the glycerine of 6 weight %, the palpus extractive of usnea of 1.5 weight %, the long and thin hot pepper berry extract of 1.5 weight %,
The hexadecanol of 1 weight %, the octadecyl alcolol of 2.5 weight %, the glycerin monostearate of 3 weight %, 16 octadecanes of 2 weight %
Base glucoside, the white oil of 6 weight %, the sad certain herbaceous plants with big flowers acid glyceride of 3 weight %, balance of water, wherein the recombined human metal
Sulfoprotein such as SEQ ID No：Shown in 1, the oligopeptides -1 such as SEQ ID No：Shown in 2.
The recombinant human metallothionein tunning extract is selected from following one or more：Recombined human metallothionein
White yeast tunning extract, Escherichia coli fermentation product extract, or lactobacillus-fermented product extract, wherein described
SEQ ID No of the recombinant human metallothionein tunning extract comprising 20-25 weight %：Recombined human metal sulphur shown in 1
Albumen.
A kind of liquid crystal type comprising recombinant human metallothionein tunning extract is provided in the fourth aspect of the present invention
The preparation method of essence, methods described comprises the steps：
Step one, the hexadecanol by 1-8 weight %, the octadecyl alcolol of 1-8 weight %, the glycerol monostearate of 1-6 weight %
Ester, being heated to 70-75 DEG C after the 16 octadecyl glucosides mixing of 1-6 weight % is completely dissolved it and maintains 75
DEG C, obtain A phases；
Step 2, the white oil by 1-10 weight %, 70- is heated to after the sad certain herbaceous plants with big flowers acid glyceride mixing of 0.5-5 weight %
75 DEG C are completely dissolved it and maintain 75 DEG C, obtain B phases；
Step 3, A phases are well mixed with B phases with 1.3-2: 1 volume ratio, and maintain 75 DEG C, obtain C phases；
Step 4, the recombinant human metallothionein tunning extract by 20 weight %, the Trenaxmine of 1 weight %, 2 weights
Measure the D-pantothenyl aleohol of %, the amino acid humectant of 3 weight %, the glycerine of 5 weight %, the palpus extractive of usnea of 1 weight %, 1 weight
Measure in the ultra-pure water of long and thin hot pepper berry extract addition 30-80 weight % of %, 70-75 DEG C is heated to after dissolving completely makes it completely molten
Solve and maintain 75 DEG C；Obtain D phases；
It is step 5, C phases and D phases is emulsified uniform in high shearing mixing emulsor with 3-4: 1 volume ratio, and stir
It is cooled to 35 DEG C；
Stirring is equal in step 6, the uniform emulsion of emulsification for adding step 5 to obtain the oligopeptides -1 of 0.001-2 weight %
It is even to obtain final product,
Wherein described recombinant human metallothionein such as SEQ ID No：Shown in 1, the oligopeptides -1 such as SEQ ID No：2 institutes
Show.
Preferably, in step 5, the high shearing mixing emulsor stir speed (S.S.) is 6500-8500r/min；
Preferably, in step 5, the high shearing mixing emulsor emulsification times are 2-6 minutes.
The recombinant human metallothionein tunning extract is selected from following one or more：Recombined human metallothionein
White yeast tunning extract, Escherichia coli fermentation product extract, or lactobacillus-fermented product extract, wherein described
SEQ ID No of the recombinant human metallothionein tunning extract comprising 20-25 weight %：Recombined human metal sulphur shown in 1
Albumen.
The fifth aspect of the present invention provides a kind of cosmetics or skin care item, and the cosmetics or skin care item include foregoing including
The essence of the tunning extract of metallothionein.
Preferably, the preparation type of the cosmetics or skin care item is aqua, creme, emulsion, gel or film forming agent.
Preferably, it is described comprising recombinant human metallothionein tunning extract in the cosmetics or skin care item
The content of essence is 0.5-30 weight %, preferably 1-20 weight %.
Preferably, the cosmetics or skin care item are also including in essential oil, alcohols, finish, pH adjusting agent and antioxidant
One or more.
Preferably, the essential oil is selected from Rosa Damascana, Lavender, refined jasmine oil, flores aurantii essential oil, laurin oil
One or more.
Preferably, the alcohols is selected from one or more in ethanol, glycerine and 1,3-BDO.
Preferably, the finish is selected from one or more in aliphatic acid, higher alcohol, atoleine and silicone oil.
Preferably, the pH adjusting agent is citric acid-sodium citrate or lactic acid-sodium lactate.
Preferably, the antioxidant be selected from Tea Polyphenols, alpha-lipoic acid, rosmanol and genistein in one kind or
It is various.
Preferably, the cosmetics or skin care item also include can be used for the compounding composition of cosmetics or skin care item, described multiple
It is made into and is selected from licorice, aloe extract, purslane extract, grape seed extract, spinach leaf extract, raspberry
One or more in ketone glucoside, prinsepia utilis royle oil.
In sum, the present invention provides following embodiments：
1. a kind of essence comprising recombinant human metallothionein tunning extract, it is characterised in that the elite
Element is included：The recombinant human metallothionein tunning extract of 0.5-30 weight %, the oligopeptides -1 of 0.001-2 weight %,
The Trenaxmine of 0.01-5 weight %, the hyaluronic acid of 1-10 weight %, the palpus extractive of usnea of 0.5-3 weight %, 0.5-3 weights
Measure the long and thin hot pepper berry extract of %, balance of water, wherein the recombinant human metallothionein such as SEQ ID No：Shown in 1, the widow
Peptide -1 such as SEQ ID No：Shown in 2.
2. a kind of liquid crystal type essence comprising recombinant human metallothionein tunning extract, it is characterised in that bag
Each component containing following percentage by weight：0.5-30 weight % recombinant human metallothionein tunning extracts, 0.001-2 weights
Oligopeptides -1, the Trenaxmine of 0.01-5 weight %, the D-pantothenyl aleohol of 1-6 weight % of amount %, the amino acid humectant of 1-5 weight %,
The glycerine of 1-10 weight %, the palpus extractive of usnea of 0.5-3 weight %, the long and thin hot pepper berry extract of 0.5-3 weight %, 1-8 weights
Measure the hexadecanol of %, the octadecyl alcolol of 1-8 weight %, the glycerin monostearate of 1-6 weight %, the 16 of 1-6 weight %
Alkyl-glucoside, the white oil of 1-10 weight %, the sad certain herbaceous plants with big flowers acid glyceride of 0.5-5 weight %, balance of water, wherein described heavy
Group human metal thioalbumen such as SEQ ID No：Shown in 1, the oligopeptides -1 such as SEQ ID No：Shown in 2.
3. the essence comprising recombinant human metallothionein tunning extract according to the 1st or 2, its feature
It is that the recombinant human metallothionein tunning extract is selected from following one or more：Recombinant human metallothionein
Yeast tunning extract, Escherichia coli fermentation product extract, or lactobacillus-fermented product extract, wherein described heavy
SEQ ID No of the group human metal thioalbumen tunning extract comprising 20-25 weight %：Recombined human metallothionein shown in 1
In vain.
4. a kind of preparation method of the liquid crystal type essence comprising recombinant human metallothionein tunning extract, described
Method comprises the steps：
Step one, the hexadecanol by 1-8 weight %, the octadecyl alcolol of 1-8 weight %, the glycerol monostearate of 1-6 weight %
Ester, being heated to 70-75 DEG C after the 16 octadecyl glucosides mixing of 1-6 weight % is completely dissolved it and maintains 75
DEG C, obtain A phases；
Step 2, the white oil by 1-10 weight %, 70- is heated to after the sad certain herbaceous plants with big flowers acid glyceride mixing of 0.5-5 weight %
75 DEG C are completely dissolved it and maintain 75 DEG C, obtain B phases；
Step 3, A phases are well mixed with B phases with 1.3-2: 1 volume ratio, and maintain 75 DEG C, obtain C phases；
Step 4, the recombinant human metallothionein tunning extract by 0.5-30 weight %, 0.01-5 weight %'s
The D-pantothenyl aleohol of Trenaxmine, 1-6 weight %, the amino acid humectant of 1-5 weight %, the glycerine of 1-10 weight %, 0.5-3 weight %
Palpus extractive of usnea, during the long and thin hot pepper berry extract of 0.5-3 weight % adds the ultra-pure water of 30-80 weight %, after dissolving completely
Being heated to 70-75 DEG C is completely dissolved it and maintains 75 DEG C；Obtain D phases；
It is step 5, C phases and D phases is emulsified uniform in high shearing mixing emulsor with 1: 3-4 volume ratio, and stir
It is cooled to 35 DEG C；
Step 6, the oligopeptides -1 of 0.001-2 weight % to be added stir in the uniform emulsion of emulsification and obtained final product,
Wherein described recombinant human metallothionein such as SEQ ID No：Shown in 1, the oligopeptides -1 such as SEQ ID No：2 institutes
Show.
5. a kind of cosmetics or skin care item, it is characterised in that the cosmetics or skin care item are comprising described in the 1st or 2
Essence comprising recombinant human metallothionein tunning extract.
6. cosmetics or skin care item according to the 5th, it is also adjusted comprising water, alcohols, finish, surfactant, pH
Section agent or antioxidant.
7. cosmetics or skin care item according to any one of the 5th or 6, its also compounded selected from licorice,
Aloe extract, purslane extract, grape seed extract, spinach leaf extract, raspberry ketone glucoside, prinsepia utilis royle oil, breast
One or more in wooden fruit oil of compounding composition.
8. cosmetics or skin care item according to the 7th, wherein described being re-dubbed point accounts for the cosmetics or skin care item
The 0.5-30% of gross mass, preferably 1-20%.
9. cosmetics or skin care item according to the 8th, the cosmetics or skin care item be made aqua, creme, emulsion,
The form of gel or film-forming agents.
10. the essence comprising recombinant human metallothionein tunning extract described in 1-3 any one exists
Prepare the application for having and subtracting in the cosmetics or skin care item that wrinkle is brightened, the tender skin of moisturizing and ultraviolet radiation preventing are acted on.
The present invention achieves following beneficial effects：
1st, essence of the present invention is right using the recombinant human metallothionein tunning extract obtained by gene recombination technology
Many kinds of metal ions has compatibility and removes free radical effect, can be effectively improved skin microcirculation, promotes skin regeneration；With good
The good tender skin of moisturizing, subtract wrinkle whitening efficacy.
2nd, essence of the present invention uses recombinant human metallothionein tunning extract for through saccharomycete, lactic acid bacteria or big
Extraction purification gained crude extract after enterobacteria fermentation, surprisingly, its anti-aging, radioresistance effect are better than simple metal sulphur egg
In vain, and crude extract be easier obtain, its yield is higher, cost is lower.
3rd, liquid crystal type essence of the present invention be acted on by the molten cause between each composition to be formed between liquid and crystal, pure solid
Intermediateness between body, the liquid crystal type essence is compared with skin sense comfort level, the permeability to skin, the moisturizing with common essence
Property more preferably, and also there is control Trenaxmine, rate of release effect of metallothionein etc..
Brief description of the drawings
From detailed description below in conjunction with the accompanying drawings, features described above of the invention and advantage will be apparent from, wherein：
Fig. 1 is white-skinned face function evaluation effect figure in embodiment 6, before wherein Fig. 1 (A, C) is the product using embodiment 1
Photo, Fig. 1 (B, D) is the design sketch after the product 60 days using embodiment 1；Fig. 1 E are to use metallothionein Essence pair
According to the photo before product (that is, comprising the Essence of simple metal sulfoprotein), Fig. 1 F are metallothionein Essence reference product
Use the design sketch after 60 days.
Specific embodiment
The present invention is further described referring to specific embodiment, it will be appreciated by those skilled in the art that this hair
It is bright to be not limited to these specific embodiments.
It should be appreciated by those skilled in the art that unless otherwise instructed, reagent used is commercially available point in following embodiments
Analyse the reagent of pure rank.
A kind of preparation comprising recombinant human metallothionein yeast tunning extract essence element of embodiment 1.
The present invention provides the side for preparing a kind of yeast tunning extract essence element comprising recombinant human metallothionein
Method, the mass percent of each component is as follows：
Table 1
Trenaxmine, amino acid humectant, palpus extractive of usnea, long and thin hot pepper berry extract are added into appropriate ultra-pure water by recipe quantity
In, it is well mixed to be made solution A；To the D-pantothenyl aleohol of the amount being sequentially added into solution A shown in table 1, recombined human metallothionein
White yeast tunning extract, oligopeptides -1, supplies excess water and stirs and obtain final product.
Wherein recombinant human metallothionein yeast tunning extract, oligopeptides -1 are by Guangzhou Ji'nan University medicine bioengineering skill
Art research and development centre prepares or synthesizes according to standard biological method, wherein the amino of recombinant human metallothionein and oligopeptides -1
Acid sequence is respectively SEQ ID No：1 and SEQ ID No：2, " the recombinant human metallothionein yeast tunning extraction of preparation
Destination protein (SEQ ID No of the thing " comprising 20-25 weight %：1)；Must extractive of usnea, long and thin hot pepper berry extract, Trenaxmine purchase
From Shaanxi Sen Fu natural products Co., Ltd；D-pantothenyl aleohol, amino acid humectant are purchased from grand hundred million Fine Chemical Co., Ltd in Guangzhou.
A kind of preparation comprising recombinant human metallothionein yeast tunning extract liquid crystal type essence of embodiment 2.
It is a kind of comprising recombinant human metallothionein yeast tunning extract liquid crystal type essence that the present invention provides preparation
Method, the mass percent of each component is as follows：
Table 2
Step one, by the hexadecanol of 3 weight %, the octadecyl alcolol of 3 weight %, the glycerin monostearate of 2 weight %, 2 weights
Being heated to 70-75 DEG C after the 16 octadecyl glucosides mixing for measuring % is completely dissolved it and maintains 75 DEG C, obtains A
Phase；
Step 2, will the white oil of 5 weight %, 2 weight % sad certain herbaceous plants with big flowers acid glyceride mix after be heated to 70-75 DEG C and make it
75 DEG C are completely dissolved and maintained, B phases are obtained；
Step 3, A phases are well mixed with B phases with 1.4: 1 volume ratio, and maintain 75 DEG C, obtain C phases；
It is step 4, the biography of the recombinant human metallothionein yeast tunning extract of 20 weight %, 1 weight % is bright
Acid, the palpus sunglo of the D-pantothenyl aleohol of 2 weight %, the amino acid humectant of 2.5 weight %, the glycerine of 5 weight %, 1 weight % are extracted
Thing, the long and thin hot pepper berry extract of 1 weight % are added in the water of 50.45 weight %, and being heated to 70-75 DEG C after dissolving completely makes its complete
Dissolve and maintain 75 DEG C；Obtain D phases；
Step 5, by C phases and D phases with 3.7: 1 volume ratio under high shearing mixing emulsor with 7000r/min rotating speeds
Emulsification 3min, and stirring is cooled to 35 DEG C or so；
Step 6, oligopeptides -1 to be added stir in the uniform emulsion of emulsification and obtained final product.
Wherein recombinant human metallothionein yeast tunning extract, oligopeptides -1 are by Guangzhou Ji'nan University medicine bioengineering skill
Art research and development centre prepares or synthesizes according to standard biological method, the amino acid sequence of recombinant human metallothionein and oligopeptides -1
Row are respectively SEQ ID No：1 and SEQ ID No：2, " recombinant human metallothionein yeast tunning extract " bag of preparation
Destination protein (the SEQ ID No of the % of weight containing 20-25：1)；Palpus extractive of usnea, long and thin hot pepper berry extract, Trenaxmine are purchased from Shan
Xi Senfu natural products Co., Ltd；D-pantothenyl aleohol, hydroxyethyl cellulose, amino acid humectant, essential oil emulsifying agent are grand purchased from Guangzhou
Hundred million Fine Chemical Co., Ltd；White oil is purchased from Guangzhou Hua Zhiwang Chemical Co., Ltd.s, hexadecanol, octadecyl alcolol, glycerol monostearate
Ester, 16 octadecyl glucosides, sad certain herbaceous plants with big flowers acid glyceride are purchased from Guangzhou Shi Ming Chemical Co., Ltd.s.
A kind of toner comprising the essence containing recombinant human metallothionein of embodiment 3.
Present invention offer prepares a kind of comprising the essence containing recombinant human metallothionein yeast tunning extract
Toner, the mass percent of each component is as follows：
Table 3
Niacinamide, hyaluronic acid, glycerine, licorice are mixed with after ultrapure water dissolves according to recipe quantity, is added thereafter
Enter the essence comprising recombinant human metallothionein yeast tunning extract of the preparation of embodiment 1, adjustment pH value to 7.0-
7.4, supplement surplus ultra-pure water stirs and obtains final product.
Wherein Tea Polyphenols, licorice are purchased from Shaanxi Sen Fu natural products Co., Ltd；It is niacinamide, hyaluronic acid, sweet
Oil, Phenoxyethanol are purchased from Hu'nan Erkang Pharmaceutical Co., Ltd..
A kind of milky lotion comprising recombinant human metallothionein lactobacillus-fermented product extract of embodiment 4.
The present invention is provided and prepares a kind of milky lotion comprising recombinant human metallothionein lactobacillus-fermented product extract, respectively
The mass percent of component is as follows：
Table 4
Stearic acid, monoglyceride, rilanit special are heated to about 80 DEG C according to recipe quantity and well mixed obtain oil phase；Will weight
Organize human metal thioalbumen lactobacillus-fermented product extract, trehalose, glycerine, aloe extract, raspberry ketone glucoside and fit
Amount water is well mixed to obtain water phase；Emulsification uniformly obtains final product milky lotion during water to be mutually slowly added into thereafter oil phase.Institute in the present embodiment
" the tunning extract of recombinant human metallothionein " for using is the extractive from fermentative of lactic acid bacteria, the mesh in the extract
Albumen (i.e. SEQ ID No：1) content is 20-25 weight %.
Wherein recombinant human metallothionein lactobacillus-fermented product extract is ground by Guangzhou Ji'nan University medical biotechnology
Study carefully development centre to be prepared according to standard biological method, wherein the amino acid sequence of destination protein is SEQ ID No：1, described
Destination protein (i.e. SEQ ID No in extract：1) content is 20-25 weight %；Oligopeptides -1 is raw by Guangzhou Ji'nan University medicine
Thing technical research development centre prepares or synthesizes according to standard biological method, and its amino acid sequence is SEQ ID No：2；Marine alga
Sugar, glycerine are purchased from Hu'nan Erkang Pharmaceutical Co., Ltd.；Stearic acid, monoglyceride, rilanit special send out trade purchased from Guangzhou Meng
Co., Ltd；Aloe extract, raspberry ketone glucoside, long and thin hot pepper berry extract are purchased from Shaanxi Sen Fu natural products Co., Ltd；
Niacinamide is purchased from Hu'nan Erkang Pharmaceutical Co., Ltd..
A kind of essence comprising recombinant human metallothionein yeast tunning extract of embodiment 5. is black to B16 cells
The influence of pigment synthesis
By logarithmic phase Murine melanoma B16 cells (being purchased from the American Type Culture Collection committee of Chinese Academy of Sciences cell bank)
Counted after being digested with pancreatin, B16 cell densities are adjusted, with 5 × 105Individual/hole is inoculated in six orifice plates, adds 2ml to contain not after 24h
With the product nutrient solution of embodiment 1 of concentration, negative control group adds the culture medium of blank 1640, using ursin (consumption) as sun
Property control.It is put into 5%CO2, 37 DEG C of incubator culture 72h, liquid is changed once in centre.After 72h, nutrient solution is discarded, added per hole
0.25% pancreatin 0.5ml digests 5min at room temperature, adds 1ml nutrient solutions to stop digestion, blows and beats into single cell suspension, takes 20ul
Make cell count, remaining cell suspension 1500r/min centrifugation 5min abandon supernatant, are eluted 2 times with PBS, and last precipitation is added
NaOH (containing 10%DMSO) solution of 1ml 1mol/L, shakes 5min, after 80 DEG C of heating water bath 30min, is transferred to 96 orifice plates, often
Hole adds 150 μ l, selects 492nm wavelength, is returned to zero with blank well, and absorbance is surveyed on ELIASA, and each experiment is repeated 3 times.
B16 cell inhibiting rate (%)=[1- (administration group OD values/administration group cell density)/(control group OD values/control
Group cell density)] × 100%, IC is carried out curve fitting and calculated using SPSS13.050Value, the results are shown in Table 5.
Essence of the table 5 comprising recombinant human metallothionein yeast tunning extract is closed to B16 intracellular melanins
Into influence
Note：* represent and compare P ＜ 0.05 with control group
The suppression synthesized to B16 intracellular melanins for the ursin solution of 100mg/L by result above visual quality concentration
Rate processed is (50.25 ± 0.13) %, and B16 intracellular melanins synthesis inhibiting rate is reached with the product of the embodiment 1 of concentration
(60.01 ± 0.35) %.Therefore the product tool of the present embodiment 1 significantly inhibits cell B16 cell ability, and in mass concentration
Dependence.
Essence white-skinned face function of the embodiment 6. comprising recombinant human metallothionein yeast tunning extract is evaluated
After qualified subject signs Informed Consent Form, determine the pigment content and skin color of its cheek and provide
Tested essence.Subject continuously uses essence of the invention (that is, the product of embodiment 1), sooner or later each daily to use 1 time,
Application after face cleaning.Other skin-lightening cosmetics are disabled during testing, it is continuous to use 30 days, after 60 days, then in kind determine
Each efficacy measures (Figure 1A-F).In addition trial employment period inquiry subject is after product using feeling and whether there is allergic phenomena etc.,
Contrast before and after itself is recorded, while setting up metallothionein essence (that is, includes the proof gold of 4 weight % in the control essence
Category sulfoprotein, the product of other compositions and corresponding content with embodiment 1 is consistent) control group.
Skin color difference meter testing result shows that it is the flat of value to use the product different time skin of face color of embodiment 1
Average and standard deviation are shown in Table 6-8.Normal human facial skin's each detected value no significant difference of color difference meter, during showing experiment
Skin color natural fluctuation is not obvious.Using product 30 days (i.e. 30d), L* values are higher than basic value (0d), difference tool to the heavy patient of color afterwards
Statistically significant (p ＜ 0.05)；A* values are substantially reducing (P ＜ 0.05) afterwards using product the 60th day (i.e. 60d), b* value changes
It is not statistically significant.From in terms of amplitude of variation, the product of embodiment 1 check colors heavy skin improvement compared with metallothionein Essence
Control group is good (data are shown in Table 6-8).
Table 6. using embodiment 1 product volunteer different time skin color value L values comparing (n=30,)
Note：Compare with 0d, * P ＜ 0.05, * * P ＜ 0.01
Table 7. using embodiment 1 product volunteer different time skin color value a* values comparing (n=30,)
Note：Compare with 0d, * P ＜ 0.05, * * P ＜ 0.01
Table 8. using embodiment 1 product volunteer different time skin color value b* values comparing (n=30,)
Note：Compare with 0d, * P ＜ 0.05, * * P ＜ 0.01
Dermal melanin index (MI) and haematochrome index (EI) average value and standard deviation are shown in Table 9-10, as a result show, make
Basic value is less than with the melanin index of the heavy patient skin of color after product the 56th day, difference is statistically significant (P ＜ 0.01), red
Pigment indices are at the 56th day obviously higher than basic value (P ＜ 0.01).
The volunteer's melanin index M I datum statistical form of table 9. (n=30,)
Note：Compare with 0d, * P ＜ 0.05, * * P ＜ 0.01
The volunteer's melanin index E I datum statistical form of table 10. (n=30,)
Note：Compare with 0d, * P ＜ 0.05, * * P ＜ 0.01
The above results absolutely prove that it is black that essence (that is, the product of embodiment 1) of the invention can be effectively improved the heavy patient of color
Pigment deposition situation, with whitening effect.From in terms of amplitude of variation, the product of embodiment 1 check colors heavy skin improvement it is golden
Category sulfoprotein Essence control group is good.This explanation prepares essence etc. and changes using recombinant human metallothionein tunning extract
Cosmetic can obtain the effect that cosmetics are prepared better than simple metal sulfoprotein, and production cost and difficulty can be reduced again, be more suitable for big
The industrial production of scale.
Although it should be understood that with reference to its exemplary embodiment, particularly shown and description is carried out to the present invention,
It should be understood by those skilled in the art that without departing substantially from the spirit of the invention as defined in appended claims
Under conditions of scope, the change of various forms and details can be wherein carried out, any of various embodiments can be carried out
Combination.
Sequence table
<110>Guangzhou and up to bio tech ltd
Biopharmaceutial Research ＆ Development Center of Jinan University
<120>Essence comprising recombinant human metallothionein tunning extract and its application in cosmetics
<130> IB168616
<160> 2
<170> PatentIn version 3.1
<210> 1
<211> 61
<212> PRT
<213>Restructuring metallothionein
<400> 1
Met Asp Pro Asn Cys Ser Cys Ala Thr Gly Gly Ser Cys Thr Cys Thr
1 5 10 15
Gly Ser Cys Lys Cys Lys Glu Cys Lys Cys Asn Ser Cys Lys Lys Ser
20 25 30
Cys Cys Ser Cys Cys Pro Met Ser Cys Ala Lys Cys Ala Gln Gly Cys
35 40 45
Ile Cys Lys Gly Ala Ser Glu Lys Cys Ser Cys Cys Ala
50 55 60
<210> 2
<211> 53
<212> PRT
<213>Oligopeptides -1
<400> 2
Asn Ser Asp Ser Glu Cys Pro Leu Ser His Asp Gly Tyr Cys Leu His
1 5 10 15
Asp Gly Val Cys Met Tyr Ile Glu Ala Leu Asp Lys Tyr Ala Cys Asn
20 25 30
Cys Val Val Gly Tyr Ile Gly Glu Arg Cys Gln Tyr Arg Asp Leu Lys
35 40 45
Trp Trp Glu Leu Arg
50
Claims (10)
1. a kind of essence comprising recombinant human metallothionein tunning extract, it is characterised in that the essence bag
Contain：The recombinant human metallothionein tunning extract of 0.5-30 weight %, oligopeptides -1, the 0.01-5 of 0.001-2 weight %
The Trenaxmine of weight %, the hyaluronic acid of 1-10 weight %, the palpus extractive of usnea of 0.5-3 weight %, the Qin of 0.5-3 weight %
Green pepper berry extract, balance of water, wherein the recombinant human metallothionein such as SEQ ID No：Shown in 1, the oligopeptides -1 such as SEQ
ID No：Shown in 2.
2. a kind of liquid crystal type essence comprising recombinant human metallothionein tunning extract, it is characterised in that comprising such as
The each component of lower percentage by weight：0.5-30 weight % recombinant human metallothionein tunning extracts, 0.001-2 weight %
Oligopeptides -1, the Trenaxmine of 0.01-5 weight %, the D-pantothenyl aleohol of 1-6 weight %, the amino acid humectant of 1-5 weight %, 1-10
The glycerine of weight %, the palpus extractive of usnea of 0.5-3 weight %, the long and thin hot pepper berry extract of 0.5-3 weight %, 1-8 weight %'s
Hexadecanol, the octadecyl alcolol of 1-8 weight %, the glycerin monostearate of 1-6 weight %, the 16 octadecyl Portugals of 1-6 weight %
Polyglycoside, the white oil of 1-10 weight %, the sad certain herbaceous plants with big flowers acid glyceride of 0.5-5 weight %, balance of water, wherein the recombined human is golden
Category sulfoprotein such as SEQ ID No：Shown in 1, the oligopeptides -1 such as SEQ ID No：Shown in 2.
3. the essence comprising recombinant human metallothionein tunning extract according to claim 1 and 2, its feature
It is that the recombinant human metallothionein tunning extract is selected from following one or more：Recombinant human metallothionein
Yeast tunning extract, Escherichia coli fermentation product extract, or lactobacillus-fermented product extract, wherein described heavy
SEQ ID No of the group human metal thioalbumen tunning extract comprising 20-25 weight %：Recombined human metallothionein shown in 1
In vain.
4. a kind of preparation method of the liquid crystal type essence comprising recombinant human metallothionein tunning extract, methods described
Comprise the steps：
Step one, the hexadecanol by 1-8 weight %, the octadecyl alcolol of 1-8 weight %, the glycerin monostearate of 1-6 weight %, 1-
Being heated to 70-75 DEG C after the 16 octadecyl glucosides mixing of 6 weight % is completely dissolved it and maintains 75 DEG C, obtains
A phases；
Step 2, the white oil by 1-10 weight %, 70-75 DEG C is heated to after the sad certain herbaceous plants with big flowers acid glyceride mixing of 0.5-5 weight %
It is completely dissolved it and maintains 75 DEG C, obtains B phases；
Step 3, A phases are well mixed with B phases with 1.3-2: 1 volume ratio, and maintain 75 DEG C, obtain C phases；
Step 4, the recombinant human metallothionein tunning extract by 0.5-30 weight %, the biography of 0.01-5 weight % are bright
Acid, the D-pantothenyl aleohol of 1-6 weight %, the amino acid humectant of 1-5 weight %, the glycerine of 1-10 weight %, the palpus of 0.5-3 weight %
Extractive of usnea, the long and thin hot pepper berry extract of 0.5-3 weight % are added in the ultra-pure water of 30-80 weight %, heating after dissolving completely
It is completely dissolved it and maintains 75 DEG C to 70-75 DEG C；Obtain D phases；
It is step 5, C phases and D phases is emulsified uniform in high shearing mixing emulsor with 1: 3-4 volume ratio, and stir cooling
To 35 DEG C；
Step 6, the oligopeptides -1 of 0.001-2 weight % to be added stir in the uniform emulsion of emulsification and obtained final product,
Wherein described recombinant human metallothionein such as SEQ ID No：Shown in 1, the oligopeptides -1 such as SEQ ID No：Shown in 2.
5. a kind of cosmetics or skin care item, it is characterised in that the cosmetics or skin care item are comprising described in claim 1 or 2
Essence comprising recombinant human metallothionein tunning extract.
6. cosmetics according to claim 5 or skin care item, it is also adjusted comprising water, alcohols, finish, surfactant, pH
Section agent or antioxidant.
7. cosmetics or skin care item according to any one of claim 5 or 6, its also compounded selected from licorice,
Aloe extract, purslane extract, grape seed extract, spinach leaf extract, raspberry ketone glucoside, prinsepia utilis royle oil, breast
One or more in wooden fruit oil of compounding composition.
8. cosmetics according to claim 7 or skin care item, wherein described being re-dubbed point accounts for the cosmetics or skin care item
The 0.5-30% of gross mass, preferably 1-20%.
9. cosmetics according to claim 8 or skin care item, the cosmetics or skin care item be made aqua, creme, emulsion,
The form of gel or film-forming agents.
10. the essence comprising recombinant human metallothionein tunning extract described in any one of claim 1-3 exists
Prepare the application for having and subtracting in the cosmetics or skin care item that wrinkle is brightened, the tender skin of moisturizing and ultraviolet radiation preventing are acted on.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109045279A (en) * | 2018-09-10 | 2018-12-21 | 因之彩生物科技（武汉）有限公司 | A kind of topical composition and its application and remedy for external use |
| CN109045280A (en) * | 2018-09-10 | 2018-12-21 | 因之彩生物科技（武汉）有限公司 | A kind of topical composition and its application and remedy for external use |
| CN109260057A (en) * | 2018-11-08 | 2019-01-25 | 妃瑶生物科技（上海）有限公司 | A kind of extracting method of prinsepia utilis royle oil composition |
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| CN101073543A (en) * | 2006-05-19 | 2007-11-21 | 湖南麓谷生物技术有限公司 | Skin-moistening composition with radiant-resisting and repairing functions |
| CN101249061A (en) * | 2008-03-17 | 2008-08-27 | 孙和平 | Hoarfrost for detoxifcation and anti-inflammation and method of preparing the same |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101073543A (en) * | 2006-05-19 | 2007-11-21 | 湖南麓谷生物技术有限公司 | Skin-moistening composition with radiant-resisting and repairing functions |
| CN101249061A (en) * | 2008-03-17 | 2008-08-27 | 孙和平 | Hoarfrost for detoxifcation and anti-inflammation and method of preparing the same |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109045279A (en) * | 2018-09-10 | 2018-12-21 | 因之彩生物科技（武汉）有限公司 | A kind of topical composition and its application and remedy for external use |
| CN109045280A (en) * | 2018-09-10 | 2018-12-21 | 因之彩生物科技（武汉）有限公司 | A kind of topical composition and its application and remedy for external use |
| CN109260057A (en) * | 2018-11-08 | 2019-01-25 | 妃瑶生物科技（上海）有限公司 | A kind of extracting method of prinsepia utilis royle oil composition |
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Address after: 510630 Guangdong city of Guangzhou province Tianhe District 82 Tao Yuk Road No. fourth layer 4075 rooms Applicant after: Guangzhou Jida Biological Technology Co., Ltd. Applicant after: Guangzhou Jinan University Medical Biotechnology Research and Development Center Co., Ltd Address before: 510630 Guangdong city of Guangzhou province Tianhe District 82 Tao Yuk Road No. fourth layer 4075 rooms Applicant before: Guangzhou Jida Biological Technology Co., Ltd. Applicant before: Medical and Biological Technology Research and Development Center, Jinan Univ. G |
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