CN106718844B - A kind of method that fast and stable spinach More female lines parent improves purity of hybrid - Google Patents
A kind of method that fast and stable spinach More female lines parent improves purity of hybrid Download PDFInfo
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- CN106718844B CN106718844B CN201710010472.9A CN201710010472A CN106718844B CN 106718844 B CN106718844 B CN 106718844B CN 201710010472 A CN201710010472 A CN 201710010472A CN 106718844 B CN106718844 B CN 106718844B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Cultivation Of Plants (AREA)
Abstract
The present invention provides a kind of methods that fast and stable spinach More female lines parent improves purity of hybrid, specific method of the invention is using More female lines to the characteristic of high temperature sensitive, fast and stable purifies female based material in a short time, accelerate breeding process, quickly solve the bottleneck problem of spinach breeding, the purity for improving cenospecies, accelerates the breeding paces of spinach cenospecies, to fill up the blank of existing spinach breeding technique.
Description
Technical field
The invention belongs to field of biotechnology, specifically, the present invention relates to a kind of fast and stable spinach More female lines parents
The method for improving purity of hybrid.
Background technique
Spinach (Spinacia oleracea L.) belongs to Amaranthaceae lamb's-quarters subfamily spinach 1 year or biennial herb plant, is weight
One of industrial crops wanted are dioecian plant, are acknowledged as sex character heredity complexity, are not easy to be selfed, parent stablizes
Slowly, hybrid seeding need to pull out the staminiferous plant of maternal system, time-consuming；Or with both sexes strain do it is maternal also have selfed seed appearance, influence
The problems such as seed purity.
It is external at present to be mainly used as maternal breeding hybrid seed using female series, but need breeding corresponding with female series
Holding system save female series, and every year will establish female series and keep breeding area, system, increase breeding cost and the production of hybrid seeds
Difficulty.And domestic spinach breeding is mainly conventional breeding, crossbreeding is at the early-stage, since the holding of female series is breeding requirement
Very high technology increases the difficulty of breeding.
Therefore, there is an urgent need in the art to develop simple, convenient and at low cost spinach breeding technique and cross-breeding method.
Summary of the invention
It is an object of the invention to using More female lines, to the characteristic of high temperature sensitive, fast and stable purifying is strong female in a short time
Property based material, accelerate breeding process, quickly solve spinach breeding bottleneck problem, improve the purity of cenospecies, accelerate spinach
The breeding paces of cenospecies, to fill up the blank of existing spinach breeding technique.As used herein, term " strong female " has ability
The conventional sense in domain, specifically " spinach More female lines " refer to spinach female strain system under normal temperature conditions, show as pure female, when
When high-temperature process, the female of female plants takes the strain for inducing a small amount of male flower.
In order to achieve the above object, the present invention provides a kind of fast and stable spinach More female lines parents to improve purity of hybrid
Method, the method includes the steps:
(1) breeding of More female lines
More female lines spinach plant is cultivated, gives high-temperature process at the florescence, seed is harvested after selfing, obtains female parent system seed；
(2) the hybrid seed production of hybrid seeds
(a) the female parent system seed and male parent system seed obtained in sowing step (1), and nursery obtains maternal plant and male parent
Plant；
(b) maternal plant obtained in Field planting step (a) and the paternal plant, the maternal plant and institute
It can be obtained the hybrid seed after stating paternal plant hybridization.
Preferably, in step (1), high-temperature process is given in Flowers ending.
Preferably, in step (1), the temperature of the high-temperature process is 30 DEG C or more；More preferably 40 DEG C or less.
Preferably, in step (1), the duration of the high-temperature process is 60 hours or more.
Preferably, in step (1), the sowing time for cultivating More female lines spinach plant is winter.
Preferably, in step (a), the time of male parent system seed sowing shifts to an earlier date 7-10 than the sowing time that female parent is seed
It.
Preferably, in step (b), the field planting ratio of the maternal plant and the paternal plant is 5-8:1.
Preferably, in step (b), Post flowering adds potash fertilizer and boron fertilizer.
The entire florescence gives 30 DEG C or more high-temperature process any time in the present invention, and trace-pollen occurs, is allowed to be selfed
Harvest seed.So all do not depart from the equivalent or modification completed under principles of this disclosure, protection of the present invention is both fallen within
Range.
It should be understood that above-mentioned each technical characteristic of the invention and having in below (eg embodiment) within the scope of the present invention
It can be combined with each other between each technical characteristic of body description, to form a new or preferred technical solution.As space is limited, exist
This no longer tires out one by one states.
Specific embodiment
Before describing the present invention, it should be understood that the present invention is not limited to the specific method and experiment conditions, because this
Class method and condition can change.It should also be understood that its purpose of the term as used herein is only that description specific embodiment, and
And it is not intended to be restrictive, the scope of the present invention will be limited only by the claims which follow.
Present invention will be further explained below with reference to specific examples.It should be understood that these embodiments are merely to illustrate the present invention
Rather than it limits the scope of the invention.Unless otherwise stated, otherwise percentage and number are calculated by weight.In following embodiment
Experimental material and reagent used can obtain unless otherwise instructed from commercially available channel.
Embodiment 1
1, the breeding of More female lines:
Area in Yantai Region winter (about 10-December 10 November) breeds warm indoor seeding More female lines seed in parent, and educates
Seedling, seedling stage press normal management, and spring (about 20 days -2 months on the 20th January) is colonized in greenhouse or greenhouse, give 30 in Flowers ending
DEG C or more accumulation high-temperature process 60 hours or more, trace-pollen will occur, after selfing, harvest seed, obtain female parent system seed.
2, hybrid seed seeding technique:
(1) seeding and seedling raising:
The female parent system seed and male parent system seed that spring breeding is received are accustomed to type of seeding seeding and seedling raising according to locality.It wants
Ask male parent sowing than female parent 10 days in advance, seedling management method is using local conventional method.
(2) cultivation mode:
Field planting is accustomed to cultivation mode according to locality, and the modes such as flat furrow, high ridge, high ridge can be used, and the generally perpendicular year 2-3 month is big
Field is colonized the production of hybrid seeds, and maternal, male parent ratio is 8:1.
(3) cultivation management method:
Production management is carried out according to conventional cultivation method after field planting, removes hybrid strain after bolting, Post flowering pays attention to fertilizer and water management,
More potassium applications and boron fertilizer, weed out the rank grass in time.
Due to female parent, system stablizes, neat and consistent, improves 30% or more than dioecism system purity；Without pulling out staminiferous plant, often
Mu can save artificial 2, save 20% or more land used, improve 50% or more hybrid seed yield, seed purity reaches 92% or more.
Embodiment 2
1, the breeding of More female lines:
District of Shanghai winter (about January 5 5-next year of December) breeds warm indoor seeding More female lines seed in parent, and
Nursery, seedling stage press normal management, and spring (about 2 months 15-March 10) is colonized in greenhouse or greenhouse, gives in Flowers ending
30 DEG C or more high temperature accumulated process 60 hours or more, trace-pollen will occur, after selfing, harvest seed, obtain female parent system seed.
2, hybrid seed seeding technique:
(1) seeding and seedling raising:
The female parent system seed and male parent system seed that spring breeding is received are accustomed to type of seeding seeding and seedling raising according to locality.It wants
Ask male parent sowing than female parent 7 days in advance, seedling management method is using local conventional method.
(2) cultivation mode:
Field planting is accustomed to cultivation mode according to locality, and the modes such as flat furrow, high ridge, high ridge can be used, and the generally perpendicular year 2-3 month is big
Field is colonized the production of hybrid seeds, and maternal, male parent ratio is 5:1.
(3) cultivation management method:
Production management is carried out according to conventional cultivation method after field planting, removes hybrid strain after bolting, Post flowering pays attention to fertilizer and water management,
More potassium applications and boron fertilizer, weed out the rank grass in time.
Due to female parent, system stablizes, neat and consistent, is that purity improves 30% or more than dioecism female parent；Without pulling out hero
Strain, can save artificial 2 per acre, save 20% or more land used, improve 50% or more hybrid seed yield, seed purity reach 92% with
On.
All references mentioned in the present invention is incorporated herein by reference, independent just as each document
It is incorporated as with reference to such.In addition, it should also be understood that, after reading the above teachings of the present invention, those skilled in the art can
To make various changes or modifications to the present invention, such equivalent forms equally fall within model defined by the application the appended claims
It encloses.
Claims (4)
1. a kind of method that fast and stable spinach More female lines parent improves purity of hybrid, which is characterized in that the method includes
Step:
(1) breeding of More female lines
More female lines spinach plant is cultivated, the sowing time is winter, gives high-temperature process in Flowers ending, harvests seed after selfing, obtain
Obtaining maternal is seed；The temperature of the high-temperature process is 30 DEG C or more and 40 DEG C or less；The accumulated time of the high-temperature process is
60 hours or more；
(2) the hybrid seed production of hybrid seeds
(a) the female parent system seed obtained in male parent system seed and step (1) is sowed, and nursery obtains maternal plant and male parent is planted
Strain；
(b) maternal plant obtained in Field planting step (a) and the paternal plant, the maternal plant and the father
It can be obtained the hybrid seed after the hybridization of this plant.
2. the method as described in claim 1, which is characterized in that in step (a), the time of male parent system seed sowing is than maternal system
The sowing time of seed shifts to an earlier date 7-10 days.
3. the method as described in claim 1, which is characterized in that in step (b), the maternal plant and the paternal plant
Field planting ratio is 5-8:1.
4. the method as described in claim 1, which is characterized in that in step (b), Post flowering adds potash fertilizer and boron fertilizer.
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| CN201710010472.9A CN106718844B (en) | 2017-01-06 | 2017-01-06 | A kind of method that fast and stable spinach More female lines parent improves purity of hybrid |
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| CN106718844A CN106718844A (en) | 2017-05-31 |
| CN106718844B true CN106718844B (en) | 2019-07-05 |
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Families Citing this family (1)
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| CN111066652B (en) * | 2019-12-30 | 2022-07-29 | 山东农业工程学院 | Breeding method of female spinach strains |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101390493A (en) * | 2008-10-24 | 2009-03-25 | 西北农林科技大学 | Seed selection method of cabbage type rape twofold sterile line |
| CN102342245A (en) * | 2010-08-06 | 2012-02-08 | 河南省农业科学院经济作物研究所 | Breeding method for pod dehiscence-resistant cytoplasmic sterile line and pod dehiscence-resistant cytoplasmic maintainer line of brassica napus |
| CN105009877A (en) * | 2015-06-26 | 2015-11-04 | 上海市农业科学院 | Method for high yield of spinach and reserving seeds for planting |
-
2017
- 2017-01-06 CN CN201710010472.9A patent/CN106718844B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101390493A (en) * | 2008-10-24 | 2009-03-25 | 西北农林科技大学 | Seed selection method of cabbage type rape twofold sterile line |
| CN102342245A (en) * | 2010-08-06 | 2012-02-08 | 河南省农业科学院经济作物研究所 | Breeding method for pod dehiscence-resistant cytoplasmic sterile line and pod dehiscence-resistant cytoplasmic maintainer line of brassica napus |
| CN105009877A (en) * | 2015-06-26 | 2015-11-04 | 上海市农业科学院 | Method for high yield of spinach and reserving seeds for planting |
Non-Patent Citations (2)
| Title |
|---|
| 耐热菠菜新品种‘沪菠1 号’;葛晨辉等;《园艺学报》;20151231;第42卷(第2期);第399-400页，尤其是第399页摘要及最后1段 |
| 菠菜性型表现及影响性型变化因素的研究;徐跃进等;《湖北农业科学》;19961231(第6期);第53-55页，尤其是第53页第1段，第55页第2.2.1节 |
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