CN106688850A - Wild-simulated cultivation matrix and device special for dendrobium tissue culture seedlings and application of wild-simulated cultivation matrix - Google Patents
Wild-simulated cultivation matrix and device special for dendrobium tissue culture seedlings and application of wild-simulated cultivation matrix Download PDFInfo
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- CN106688850A CN106688850A CN201611145153.0A CN201611145153A CN106688850A CN 106688850 A CN106688850 A CN 106688850A CN 201611145153 A CN201611145153 A CN 201611145153A CN 106688850 A CN106688850 A CN 106688850A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
- A01G31/02—Special apparatus therefor
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/40—Fertilisers incorporated into a matrix
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention provides a wild-simulated cultivation matrix special for dendrobium tissue culture seedlings. The cultivation matrix comprises degreased pine barks, brick and tile rubbles, dry mosses and nutritional additives. The forest wild-simulated cultivation matrix has the advantages that the problem of seedling death during dendrobium tissue culture seedling transplanting is solved, a good forest living environment is built for dendrobium, moisture conservation can be achieved effectively, and the growth requirements of 'through, leakage and penetration'of dendrobium roots are satisfied.
Description
Technical field
The present invention relates to a kind of crop-planting method, more specifically it is related to a kind of dendrobe tissue culture seedling to imitate wild cultivation special
With matrix, device and application.
Technical background
The stem of noble dendrobium is orchid family Dendrobium Sw, and the whole world there are about Dendrobium initial species 1 500~1 600 kind, be distributed mainly on Asia
Continent subtropical and tropical zones and Australia, most of integrated distributions are in l5 ° 30 '~25 ° 21 ' area of north latitude, and the country is main
It is distributed in Yunnan, Guangxi, Guangdong, Sichuan, Hainan and Taiwan etc. and saves (area).The stem of noble dendrobium quality that Yunnan is produced with the western regions of the Yunnan Province and South-West Yunnan
Most preferably.《Yunnan plant will》In 46 kinds of stems of noble dendrobium including be found in Changning County.Taoist school that Tang was opened between the first year is classical《Taoist Scriptures》
Once dendrobium candidum was classified as " first of Chinese nine big celestial grass ", by dendrobium candidum, Herba Saussureae Involueratae, three double ginsengs, 120 years head
Crow, the Poria cocos desert cistanche of cycle of sixty years, remote mountains ganoderma lucidum, seabed pearl, Cordyceps sinensis etc. are classified as Chinese nine big celestial grass.Dendrobium candidum is even more stone
Top grade in dry measure used in former times.
Dendrobium candidum (scientific name：Dendrobium officinale Kimura et Migo)：Stem is upright, cylinder, long
9-35 centimetres, thick 2-4 millimeters, branch, does not have more piece；Ye Erlie, papery, round shape lanceolar long, edge and middle rib are often with pale purple
Color.Raceme often sends from the old stem top of the leaf that fallen, and has 2-3 flower；Petal piece dry film matter, shallow white is avette, 5-7 long
Millimeter, sepal and petal yellow green, nearly similar, round shape lanceolar long, lip white, base portion have the callosity of 1 green or yellow
Body, ovum shape lanceolar is more slightly shorter than sepal, middle part reflexed.Stamen post yellow green, is about 3 millimeters, and tip both sides respectively have 1 purple point；Medicine
Cap white, ovate triangle long is about 2.3 millimeters, and the nearly sharp point in top and 2 are split.The month at florescence 3-6.
The stem of noble dendrobium avoids dry, fearness ponding, especially needs adequate water when sprouting starts and sprouts and formed to new root.But it is excessively damp
It is wet, such as low temperature, it is easy to cause rotten.When becoming a fine day xeothermic, in addition to watering, air higher is kept toward water spray more than ground
Humidity.Evergreen stem of noble dendrobium class can keep adequate water in the winter time, but the fallen leaves class stem of noble dendrobium can be dried suitably, keep air higher wet
Degree.Stem of noble dendrobium happiness grows in warm, moist, half cloudy half light environment, is more than with more than 1000 millimeters of annual rainfall, air humidity
80%th, grown in subtropical zone remote, thickly forested mountains of 1 monthly mean temperature higher than 8 DEG C and be preferred, it is very not strict to clay fertilizer requirement, it is wild to exist more
Grown on loose and thick bark or trunk, what is had is also grown in crack of stone.Annual the end of spring and the beginning of summer, taken out on biennial stem top section
Go out inflorescence, Post flowering grows sprouting and develops into stem from stem foot, and autumn and winter enters people's rest period.
Dendrobe tissue culture nursery work is started in 7O age Mos to the beginning of the eighties in 20th century.Tissue culture is certain for utilizing the stem of noble dendrobium
Individual slices of organs, seedling is cultivated by tissue culture room.The method investment is big, with high content of technology, needs professional and technical personnel to complete.Its
Advantage is fast reproduction speed, and purity is high, disease-free.Above need to be answered by can just be put into production after professional and technical personnel's hardening in application
With initial time of producing effects is needed 2~3 years.
Extensively, such as Qi wood, numb chestnut, walnut, Chinese toon be wooden, the nearly all broad-leaved arbor of pears for the attached main plant of the stem of noble dendrobium, especially with
Deciduous tree is more, and needlebush is grown nonparasitically upon another plant less.Should select many trunk branches, tree big (live body pseudo-wild cultivating), sturdy, skin depth,
Being difficult decortication, bark has the plant transplantations such as crack.The nutrition of dendrobium candidum aerial root is fixed in air mainly by the blue bacterium of root system
Nomadic nitrogen, but the tissue-cultured seedling for just having transplanted, applying appropriate fertilizer can promote new root to increase rapidly, advantageously in
The plantation survival rate of the stem of noble dendrobium.
The content of the invention
To realize the problem of the woodland pseudo-wild cultivating of the tissue culture stem of noble dendrobium second, the invention provides a kind of candidum tissue culturing seedling
Woodland imitates wild cultivation dedicated substrate, the dedicated substrate by degreasing pine bark, brickbat tile fragment, dry liver moss and nourishing additive agent
Composition.
Han Liang≤50% of active ingredient in the nourishing additive agent, the active ingredient is by NO, P2O5、K2O、Fe2O3、
Cu, B, Zn and Mo are constituted.
Active ingredient NO, P in nourishing additive agent2O5、K2O、Fe2O3, Cu, B, Zn and Mo weight ratio be 100:30:50:
10:3:2:1:1。
The dedicated substrate is 30 by degreasing pine bark, brickbat tile fragment, the weight ratio for drying liver moss and nourishing additive agent:
10:5:2。
The preparation of the dedicated substrate and method are comprised the following steps：
1) pine bark is made the fritter of particle diameter 1.5-4cm, as 2-3h is decocted in boiling water, is leached, dried and obtain degreasing
Pine bark；
2) discarded brick and tile are made the fritter of 1.5-4cm, are dried, degreasing pine bark, brickbat tile fragment are weighed according to ratio
And nourishing additive agent, by nourishing additive agent water dissolves, in osmotic absorption to degreasing pine bark and brickbat tile fragment, dry and obtain brick
Wooden mixture；
3) weighed to dry liver moss and be mixed into brick wood mixture according to ratio and prepared for the imitative open country of candidum tissue culturing seedling
The raw dedicated substrate cultivated；
4) when in use, will be attached on trunk by the dendrobe tissue culture seedling root system of hardening, the special device breathed freely with drainage
By the dedicated substrate outside for being fixed on dendrobe tissue culture seedling root system, the stem and leaf of dendrobe tissue culture seedling expose.
A kind of special purpose device that wild cultivation is imitated for candidum tissue culturing seedling, the special special purpose device is by for fixing
The string bag of matrix and the fixing band composition for being fixed on the string bag on trunk, are wherein set on the string bag by being breathed freely for drainage
Treatment hole, the top of the string bag is longer than below.
Described candidum tissue culturing seedling woodland imitates wild cultivation dedicated substrate answering in woodland stem of noble dendrobium pseudo-wild cultivating
With.
The method have the benefit that：The dendrobium candidum woodland that the present invention is provided puts wild cultivation dedicated substrate, solves
Determine the seedling death phenomenon that can occur in dendrobe tissue culture transplantation of seedlings, be that the stem of noble dendrobium constructs an excellent woodland living environment, energy
Enough growth requirements for effectively realizing moisture self-restraint and stem of noble dendrobium coring " logical, leakage, thoroughly ".
Figure of description
Fig. 1 imitates the special purpose device structural representation of wild cultivation for candidum tissue culturing seedling；
Fig. 2 imitates the use schematic diagram of the special purpose device of wild cultivation for candidum tissue culturing seedling；
1 string bag, 1-1 tops, 1-2 is following, 3 fixing bands, 2 treatment holes.
Specific embodiment
The effect of the different nutritional agents of embodiment 1 and matrix to dendrobium candidum imitating wild planting early growth is investigated
1st, different substrates influence on the early growth of dendrobium candidum imitating wild planting
1. pine bark is made the fritter of particle diameter 1.5-4cm, as 2-3h is decocted in boiling water, is leached, dried and obtain degreasing
Pine bark；Discarded brick and tile are made the fritter of particle diameter 1.5-4cm, are dried；Liver moss is dried, sealing is piled up to taking off cyan.
By trace elements such as potassium nitrate, ammonium sulfate, ferric sulfate, copper sulphate, zinc sulfate and potassium dihydrogen phosphate addition B and Mo,
Composition NO, P2O5、K2O、Fe2O3, Cu, B, Zn and Mo weight ratio be 100:30:50:10:3:2:1:1 nourishing additive agent, its
In, NO, P2O5、K2O、Fe2O3, Cu, B, Zn and Mo constitute active ingredient Han Liang≤50%.According to degreasing pine bark, broken brick
Block, the weight ratio for drying liver moss and nourishing additive agent are 30:10:5:2 ratio weighs raw material, and degreasing pine tree is weighed according to ratio
Skin, brickbat tile fragment and nourishing additive agent, by nourishing additive agent water dissolves, osmotic absorption to degreasing pine bark and brickbat tile fragment
In, dry and obtain brick wood mixture, liver moss, degreasing pine bark, brickbat tile fragment and nourishing additive agent are mixed；Prepare special
Matrix.
2. pine bark is made the fritter of particle diameter 1.5-4cm, as 2-3h is decocted in boiling water, is leached, dried and obtain degreasing
Pine bark；Discarded brick and tile are made the fritter of particle diameter 1.5-4cm, are dried；Liver moss is dried, sealing is piled up to taking off cyan.
Potassium nitrate and potassium dihydrogen phosphate are mixed into N:P2O5:K2O=25:13:13 composite fertilizer, according to degreasing pine tree
Skin, brickbat tile fragment, the weight ratio for drying liver moss and composite fertilizer are 30:10:5:2 ratio weighs raw material, is weighed according to ratio de-
Fat pine bark, brickbat tile fragment and nourishing additive agent, by nourishing additive agent water dissolves, osmotic absorption to degreasing pine bark and brickbat
In tile fragment, dry and obtain brick wood mixture, liver moss, degreasing pine bark, brickbat tile fragment and nourishing additive agent are mixed；Prepare
Control group A.
3. pine bark is made the fritter of particle diameter 1.5-4cm, as 2-3h is decocted in boiling water, is leached, dried and obtain degreasing
Pine bark；Kaolinite is made the fritter of particle diameter 1.5-4cm.Potassium nitrate and potassium dihydrogen phosphate are mixed into N:P2O5:K2O=
25:13:13 composite fertilizer, the weight ratio according to degreasing pine bark, kaolinite and composite fertilizer is 30:10:2 ratio weighs original
Material, weighs degreasing pine bark and nourishing additive agent, by nourishing additive agent water dissolves, osmotic absorption to degreasing pine tree according to ratio
Pi Zhong, is dried, and degreasing pine bark kaolinite and nourishing additive agent are mixed；Prepare control group B.
4. pine bark is made the fritter of particle diameter 1.5-4cm, as 2-3h is decocted in boiling water, is leached, dried and obtain degreasing
Pine bark.Just potassium nitrate, ammonium sulfate, ferric sulfate, copper sulphate, zinc sulfate and potassium dihydrogen phosphate add the micro units such as B and Mo
Element, constitutes NO, P2O5、K2O、Fe2O3, Cu, B, Zn and Mo weight ratio be 100:30:50:10:3:2:1:1 nourishing additive agent,
Wherein, NO, P2O5、K2O、Fe2O3, Cu, B, Zn and Mo constitute active ingredient Han Liang≤50%.According to degreasing pine bark, brickbat
Tile fragment, the weight ratio for drying liver moss and composite fertilizer are 30:10:5:2 ratio weighs raw material, and degreasing pine bark is weighed according to ratio
And nourishing additive agent, nourishing additive agent water dissolves in osmotic absorption to degreasing pine bark, are dried；Prepare control group
C。
To be randomly assigned to be 4 groups by the candidum tissue culturing seedling of hardening (every clump of seedling >=3 plant, plant height >=8 centimetre), often
Group 100 clumps, respectively dedicated substrate group treatment group, control group A treatment, control group B treatment, control group C treatment.Each treatment point
Above-mentioned acquisition dedicated substrate, control group A, control group B and control group C matrix are not used with the amount of every seedling 300g, use it is special
The wild mode in hardening side is planted on banyan trunk (as shown in Figure 2) with device (as shown in Figure 1).In strain Cong Dingzhi
Afterwards, according to weather condition, run into and continuously become a fine day, need to water sooner or later daily.After 3 months, after new root grows and is fixed on rock, can
The less number of times for watering and applying fertilizer, makes it grow naturally.Statistics each treatment survival rate and individual plant iron sheet stone after six months
The total biomass of dry measure used in former times strain clump.It is specific as shown in table 1.
Influence of the different disposal of table 1 to stem of noble dendrobium germination and biomass
| Treatment group | Survival rate (from) | Average biomass of individual tree (g) |
| The treatment of dedicated substrate group | 96 | 389.35±35.27 |
| Control group A treatment | 88 | 189.57±25.16 |
| Control group B treatment | 71 | 270.45±35.39 |
| Control group C treatment | 90 | 289.23±25.65 |
It can be seen from the data described in table 1, the dedicated substrate for just having this patent to provide not only can effectively improve the stem of noble dendrobium
The survival rate of underwood planting, also has obvious action simultaneously for dendrobium candidum yield is improved.
Claims (7)
1. a kind of candidum tissue culturing seedling woodland imitates wild cultivation dedicated substrate, it is characterised in that：The dedicated substrate is by degreasing
Pine bark, brickbat tile fragment, dry liver moss and nourishing additive agent composition.
2. candidum tissue culturing seedling woodland according to claim 1 imitates wild cultivation dedicated substrate, it is characterised in that：It is described
Han Liang≤50% of active ingredient in nourishing additive agent, the active ingredient is by NO, P2O5、K2O、Fe2O3, Cu, B, Zn and Mo group
Into.
3. candidum tissue culturing seedling woodland according to claim 2 imitates wild cultivation dedicated substrate, it is characterised in that：Battalion
Support active ingredient NO, P in additive2O5、K2O、Fe2O3, Cu, B, Zn and Mo weight ratio be 100:30:50:10:3:2:1:1.
4. candidum tissue culturing seedling woodland according to claim 1 imitates wild cultivation dedicated substrate, it is characterised in that：It is described
Dedicated substrate is 30 by degreasing pine bark, brickbat tile fragment, the weight ratio for drying liver moss and nourishing additive agent:10:5:2.
5. candidum tissue culturing seedling woodland according to claim 4 imitates wild cultivation dedicated substrate, it is characterised in that：It is described
The preparation of dedicated substrate and method are comprised the following steps：
1）Pine bark is made the fritter of particle diameter 1.5-4cm, as 2-3h is decocted in boiling water, is leached, dried and obtain degreasing pine tree
Skin；
2）Discarded brick and tile are made the fritter of 1.5-4cm, are dried, degreasing pine bark, brickbat tile fragment and battalion are weighed according to ratio
Additive is supported, by nourishing additive agent water dissolves, in osmotic absorption to degreasing pine bark and brickbat tile fragment, dries that to obtain brick wood mixed
Compound；
3）Weighed to dry liver moss and be mixed into brick wood mixture according to ratio and prepare and imitate wild training for candidum tissue culturing seedling
The dedicated substrate educated；
4）When in use, will be attached on trunk by the dendrobe tissue culture seedling root system of hardening, the special device breathed freely with drainage will be special
With the matrix outside for being fixed on dendrobe tissue culture seedling root system, the stem and leaf of dendrobe tissue culture seedling expose.
6. a kind of special purpose device that wild cultivation is imitated for candidum tissue culturing seedling, it is characterised in that：The special purpose device by with
In the string bag of fixed matrix（1）With for by the string bag（1）It is fixed on the fixing band on trunk（3）Composition, the wherein string bag（1）On set
Put the treatment hole by being breathed freely for drainage（2）, the top of the string bag（1-1）It is longer than following（1-2）.
7. the candidum tissue culturing seedling woodland according to claim 1-5 imitates wild cultivation dedicated substrate in the imitative open country of the woodland stem of noble dendrobium
Application in raw cultivation.
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| CN201611145153.0A CN106688850A (en) | 2016-12-13 | 2016-12-13 | Wild-simulated cultivation matrix and device special for dendrobium tissue culture seedlings and application of wild-simulated cultivation matrix |
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| CN201611145153.0A CN106688850A (en) | 2016-12-13 | 2016-12-13 | Wild-simulated cultivation matrix and device special for dendrobium tissue culture seedlings and application of wild-simulated cultivation matrix |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107494058A (en) * | 2017-09-30 | 2017-12-22 | 宁波神乙草生物科技有限公司 | A kind of dendrobium candidum pastes tree planting device |
| CN107691211A (en) * | 2017-11-28 | 2018-02-16 | 顿春垚 | A kind of stem of noble dendrobium bionic cultivation fetters bag |
| CN110583457A (en) * | 2019-09-28 | 2019-12-20 | 李洪文 | Wild-imitating planting method for dendrobium |
| CN110915564A (en) * | 2019-12-18 | 2020-03-27 | 锦屏贵枫堂生态农业开发有限公司 | Method for cultivating imitating wild dendrobium officinale in fir forest and trunk fixing device thereof |
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| CN105265295A (en) * | 2015-12-04 | 2016-01-27 | 贵州省植物园 | Dendrobium officinale culture substrate and preparation method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105265295A (en) * | 2015-12-04 | 2016-01-27 | 贵州省植物园 | Dendrobium officinale culture substrate and preparation method |
Non-Patent Citations (1)
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| 陈美钦: "铁皮石斛无土栽培基质选择与营养液配方的优化", 《中国硕士学位论文全文数据库（电子期刊）》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107494058A (en) * | 2017-09-30 | 2017-12-22 | 宁波神乙草生物科技有限公司 | A kind of dendrobium candidum pastes tree planting device |
| CN107691211A (en) * | 2017-11-28 | 2018-02-16 | 顿春垚 | A kind of stem of noble dendrobium bionic cultivation fetters bag |
| CN110583457A (en) * | 2019-09-28 | 2019-12-20 | 李洪文 | Wild-imitating planting method for dendrobium |
| CN110583457B (en) * | 2019-09-28 | 2021-09-14 | 李洪文 | Wild-imitating planting method for dendrobium |
| CN110915564A (en) * | 2019-12-18 | 2020-03-27 | 锦屏贵枫堂生态农业开发有限公司 | Method for cultivating imitating wild dendrobium officinale in fir forest and trunk fixing device thereof |
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