CN106636359B - 基于sanger测序测重组HPV腺病毒基因组的方法 - Google Patents
基于sanger测序测重组HPV腺病毒基因组的方法 Download PDFInfo
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- CN106636359B CN106636359B CN201611022552.8A CN201611022552A CN106636359B CN 106636359 B CN106636359 B CN 106636359B CN 201611022552 A CN201611022552 A CN 201611022552A CN 106636359 B CN106636359 B CN 106636359B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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Abstract
Description
基因组DNA | 1.0ul |
10×LA Buffer(含2.5mM Mg2+) | 5.0ul |
LA Taq聚合酶(5u/μL) | 1.0ul |
dNTP(10mM) | 1.0ul |
正向引物(10uM) | 1.5ul |
反向引物(10uM) | 1.5ul |
ddH2O | 39.0ul |
总体积 | 50.0ul |
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CN106636359A CN106636359A (zh) | 2017-05-10 |
CN106636359B true CN106636359B (zh) | 2020-08-18 |
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Families Citing this family (1)
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CN110894555B (zh) * | 2019-12-10 | 2023-05-23 | 奥维森基因科技(天津)有限公司 | 基于Sanger测序的黄热病毒全基因组测序方法、成套引物对和应用 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101497891A (zh) * | 2008-02-03 | 2009-08-05 | 深圳市源兴生物医药科技有限公司 | 一种新型重组病毒载体及其应用 |
CN102002105A (zh) * | 2009-09-03 | 2011-04-06 | 中国疾病预防控制中心病毒病预防控制所 | Hpv 16型e7e6融合蛋白基因、表达载体、方法、细胞和用途 |
CN105331726A (zh) * | 2015-12-01 | 2016-02-17 | 上海派森诺生物科技股份有限公司 | 基于sanger测序人线粒体基因组的方法 |
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2016
- 2016-11-15 CN CN201611022552.8A patent/CN106636359B/zh active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101497891A (zh) * | 2008-02-03 | 2009-08-05 | 深圳市源兴生物医药科技有限公司 | 一种新型重组病毒载体及其应用 |
CN102002105A (zh) * | 2009-09-03 | 2011-04-06 | 中国疾病预防控制中心病毒病预防控制所 | Hpv 16型e7e6融合蛋白基因、表达载体、方法、细胞和用途 |
CN105331726A (zh) * | 2015-12-01 | 2016-02-17 | 上海派森诺生物科技股份有限公司 | 基于sanger测序人线粒体基因组的方法 |
Non-Patent Citations (4)
Title |
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Effcient construction of recombinant adenovirus expression vector of the Qinchuan cattle LYRM1 gene;Y.K. Li等;《Genetics and Molecular Research》;20150814;第14卷(第3期);第9469-9477页 * |
IL-31重组腺病毒载体的构建及鉴定;黄俊琼等;《临床和实验医学杂志》;20080630;第7卷(第6期);第17-19页 * |
In vivo Antitumor Effect of an HPV-specific Promoter driving IL-12 Expression in an HPV 16-positive Murine Model of Cervical Cancer;Victor Hugo Bermudez-Morales等;《Journal of Cancer》;20160927;第7卷(第14期);第1950-1959页 * |
人KLK1和EGFP双顺反子重组腺病毒构建及其在血管平滑肌细胞中的表达;余惠珍等;《中国病理生理杂志》;20081231;第24卷(第2期);第411-416页 * |
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Denomination of invention: A method for detecting recombinant HPV adenovirus genome based on sanger sequencing Effective date of registration: 20220801 Granted publication date: 20200818 Pledgee: The Bank of Shanghai branch Caohejing Limited by Share Ltd. Pledgor: SHANGHAI PERSONAL BIOTECHNOLOGY Co.,Ltd. Registration number: Y2022310000157 |
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