CN106614107A - Method for performing bee larva incubation and collection by means of visualized bee incubation beehive - Google Patents
Method for performing bee larva incubation and collection by means of visualized bee incubation beehive Download PDFInfo
- Publication number
- CN106614107A CN106614107A CN201611221243.3A CN201611221243A CN106614107A CN 106614107 A CN106614107 A CN 106614107A CN 201611221243 A CN201611221243 A CN 201611221243A CN 106614107 A CN106614107 A CN 106614107A
- Authority
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- China
- Prior art keywords
- beehive
- apiss
- hatch
- bee
- honeybee
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000011534 incubation Methods 0.000 title claims abstract description 15
- 238000000034 method Methods 0.000 title claims abstract description 13
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 56
- 241000256844 Apis mellifera Species 0.000 claims abstract description 47
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 28
- 210000000952 spleen Anatomy 0.000 claims abstract description 25
- 238000001816 cooling Methods 0.000 claims abstract description 4
- 241000256836 Apis Species 0.000 claims description 65
- 229920005479 Lucite® Polymers 0.000 claims description 28
- 239000004926 polymethyl methacrylate Substances 0.000 claims description 28
- 230000012447 hatching Effects 0.000 claims description 21
- 230000000007 visual effect Effects 0.000 claims description 17
- 229910000831 Steel Inorganic materials 0.000 claims description 12
- 239000011148 porous material Substances 0.000 claims description 12
- 239000010959 steel Substances 0.000 claims description 12
- 238000012800 visualization Methods 0.000 claims description 9
- 229910001220 stainless steel Inorganic materials 0.000 claims description 7
- 239000010935 stainless steel Substances 0.000 claims description 7
- 238000002347 injection Methods 0.000 claims description 6
- 239000007924 injection Substances 0.000 claims description 6
- 230000002085 persistent effect Effects 0.000 claims description 6
- 239000011521 glass Substances 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 4
- 206010002091 Anaesthesia Diseases 0.000 claims description 3
- 230000037005 anaesthesia Effects 0.000 claims description 3
- 241000257303 Hymenoptera Species 0.000 abstract 2
- 244000144987 brood Species 0.000 abstract 1
- 210000001520 comb Anatomy 0.000 abstract 1
- 230000003247 decreasing effect Effects 0.000 abstract 1
- 208000004619 Inert Gas Narcosis Diseases 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 235000012907 honey Nutrition 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 230000032683 aging Effects 0.000 description 1
- 231100000132 chronic toxicity testing Toxicity 0.000 description 1
- 230000002493 climbing effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 231100000566 intoxication Toxicity 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K49/00—Rearing-boxes; Queen transporting or introducing cages
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses a method for performing bee larva incubation and collection by means of a visualized bee incubation beehive. The method comprises the following steps of 1 bee spleen placement, 2 bee spleen incubation, 3 cooling, 4 dead bee removal, 5 bee anesthetization and 6 bee collection. The visualized bee incubation beehive, an incubator for adjusting temperature and humidity and a nitrogen supply device are used for achieving bee incubation and collection. The beehive for laboratory incubation is improved, the size of the beehive is decreased, the beehive is convenient to carry and can be placed into the incubator, the temperature and humidity of incubation are controlled by means of the incubator, and meanwhile the phenomenon that brood combs inside the beehive get out of the beehive can be monitored in the whole process; in addition, when bees are collected, by introducing nitrogen into small holes in a cover of the beehive, bees are anesthetized to be collected.
Description
Technical field
The present invention relates to bee raising technical field, specially a kind of to carry out young honeybee hatching using visual Apiss hatching beehive
And the method collected.
Background technology
Apiss chronic toxicity test is longer because of the test period, in order to avoid Apiss cause intoxication conditions not because of age in days difference
It is consistent or because of aging dead these differences, it is general using phase young honeybee while just going out several days of spleen.In order to obtain children of the same period
Honeybee, first lays eggs every king in outdoor wooden beehive, when will going out room, takes out the sub- spleen of capping and be transferred to reality from wooden beehive
Indoor wooden beehive is tested, and is hatched under appropriate humiture, collects for trying for 4-5 days after Apiss young honeybee goes out room
Test.
Traditional wooden beehive volume is larger, it is difficult to move, and the bad observation sub- spleen of beehive inner cover goes out room situation；
And traditional Apiss are collected in its transfer process, as manual operation sound is big, Apiss easily escape everywhere, are likely to result in honey
Attack of the honeybee to technical staff, it is also possible to cause just to go out the spleen young honeybee of several days and be subject to different degrees of infringement.Meanwhile, technical staff
Needs carry out Apiss for several times and collect, and workload is big.
The content of the invention
It is an object of the invention to provide a kind of hatch the method that beehive carries out young honeybee hatching and collection using visual Apiss,
To solve the problems, such as to propose in above-mentioned background technology, had an advantageous effect in that；Present invention utilizes visualization Apiss hatching
Beehive, the couveuse for adjusting humiture and nitrogen supply (NS) device this package unit are realized the hatching of Apiss and collect work
Make.By the beehive for being modified to laboratory hatching, reduce its volume so as to be easy to carry, and can be placed in couveuse, utilize
Couveuse is collecting honey come the humiture for controlling to hatch in addition while room situation can be gone out with sub- spleen in complete monitoring beehive
Nitrogen narcosis Apiss can be passed through to be collected by the aperture of beehive case lid during honeybee.
For achieving the above object, the present invention provides following technical scheme：A kind of visual Apiss hatch beehive, including beehive sheet
Body：The top of the beehive body is open type open-type, is installed with handle panel on the outer wall of the beehive body both sides,
Second card article at the top of the beehive body is tightly connected by single side self sponge strip with the junction of lucite case lid,
The second card article and the first card article are fixed with the inwall of the beehive bodies top opening, the second card article is located at the upper of the first card article
Side, is fixed with the top of the lucite case lid on handle, and the lucite case lid of handle side and is provided with logical nitrogen pore
And rustless steel web plate is connected with the first card article of passage, the lucite case lid and beehive body interior, it is described stainless
Steel net plate be by two pieces of left side plates, two pieces in front and back side plate and stainless steel silk constitute, and handle is installed on stainless steel silk.
It is a kind of to hatch the method that beehive carries out young honeybee hatching and collection using visual Apiss, comprise the steps：Step one,
Place honeybee spleen；Step 2, the hatching of honeybee spleen；Step 3, cooling；Step 4, clears up dead Apiss；Step 5, anaesthetizes Apiss；Step
Six, Apiss are collected；It is characterized in that：
In step one, the bee variety spleen that will go out room is put in visualization Apiss incubator, rustless steel Net plate cover is covered；
In step 2, the visualization Apiss incubator with the bee variety spleen that will go out room is put in couveuse and is incubated
Change, incubation temperature is 30-34 DEG C, and humidity is 70-90%;
In step 3, Apiss hatch latter first day to the 4th day temperature and reduce by 1 DEG C all in original temperature, relative humidity
It is constant；
In step 4, the 4th or the 5th day after Apiss hatch, honeybee spleen is gently released, the dead Apiss dropped in bottom
Pour out, after covering lucite case lid；
In step 5, nitrogen is passed through from the aperture of honeybee lid carries out anesthesia and the collection work of Apiss, wherein nitrogen injection
Flow is 10-15L/min, and the persistent period is 60s ~ 120s；
In step 6, postanesthetic Apiss are collected.
Preferably, the lucite case lid and beehive body are made up of the component of lucite material, and beehive sheet
Body is all around bonded with single side self sponge strip with the junction of lower shoe.
Preferably, aperture of the aperture of the logical nitrogen pore more than passage, and the aperture of logical nitrogen pore is 10mm.
Preferably, the length and width and thickness of second card article be respectively 70mm, 5mm and 6mm, the length and width of the first card article and
Thickness is respectively 70mm, 15mm and 6mm.
Preferably, the length and width of the beehive body and high respectively 495mm, 80mm and 270mm, lucite case lid
Length and width is 495mm and 80mm.
Preferably, second card article and the edge distance of the opening of beehive body are 15mm.
Preferably, in the step 2, incubation temperature is 33 ± 1 DEG C；Humidity is 80 ± 5%.
Preferably, in the step 5, the flow of nitrogen injection is 12L/min；Persistent period is 100s.
Compared with prior art, the invention has the beneficial effects as follows：Visualization Apiss hatching beehive has small volume, is easy to take
The advantages of band and observability；It is unfavorable for Apiss climbing activity on wall simultaneously, the Apiss primary limitation that is obstructed due to creeping is in honeybee
Take action on honeybee spleen in case；In addition beehive volume is matched with a honeybee spleen, can place several beehives simultaneously, significantly in couveuse
Space and brooding time are saved, on the beehive case lid of hatching, have an aperture to be 1cm aperture can be passed through nitrogen by aperture
Gas anaesthetizes Apiss to carry out the collection of Apiss；Present invention utilizes visualization Apiss hatching beehive, for adjusting incubating for humiture
Change device and nitrogen supply (NS) device this package unit realizes hatching and the collection work of Apiss.By being modified to laboratory hatching
Beehive, reduce its volume so as to be easy to carry, and can be placed in couveuse, controlled using couveuse hatch humiture,
Simultaneously room situation can be gone out with sub- spleen in complete monitoring beehive, can be led to by the aperture of beehive case lid when Apiss are collected in addition
Enter nitrogen narcosis Apiss to be collected.
Description of the drawings
Fig. 1 is overall structural representation of the invention；
Fig. 2 is the structural representation of lucite case lid of the present invention；
Fig. 3 is the structural representation of rustless steel web plate of the present invention；
Fig. 4 is the structural representation of beehive body of the present invention；
Fig. 5 is the schematic flow sheet of beehive body of the present invention；
In figure：1- lucite case lids；2- handles；3- leads to nitrogen pore；4- sides fixed plate；5- beehive bodies；6- stainless (steel) wires
Plate；7- left side plates；Side plate before and after 8-；9- shakes hands；10- passages.
Specific embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation is described, it is clear that described embodiment is only a part of embodiment of the invention, rather than the embodiment of whole.It is based on
Embodiment in the present invention, it is every other that those of ordinary skill in the art are obtained under the premise of creative work is not made
Embodiment, belongs to the scope of protection of the invention.
Refer to Fig. 1-5, a kind of embodiment that the present invention is provided：A kind of visual Apiss hatch beehive, including beehive body
5：The top of beehive body 5 is open type open-type, and handle panel 4, beehive sheet are installed with the outer wall of 5 both sides of beehive body
Second card article 11 at the top of body 5 is tightly connected by single side self sponge strip with the junction of lucite case lid 1, beehive sheet
The second card article 11 and the first card article 12 are fixed with 5 open-topped inwall of body, the second card article 11 is located at the upper of the first card article 12
Side, the top of lucite case lid 1 is fixed with handle 2, and the lucite case lid 1 of 2 side of handle and is provided with logical nitrogen pore 3
With passage 10, on the first card article 12 inside lucite case lid 1 and beehive body 5, rustless steel web plate 6, rustless steel are connected with
Web plate 6 is that side plate 8 and stainless steel silk are constituted in front and back by 7, two pieces of two pieces of left side plates, and handle 9 is provided with stainless steel silk, is had
Machine glass case lid 1 and beehive body 5 are made up of the component of lucite material, and beehive body 5 all around with lower shoe
Junction be bonded with single side self sponge strip, lead to the aperture of the aperture more than passage 10 of nitrogen pore 3, and logical nitrogen pore 3
Aperture is 10mm, and the length and width and thickness of the second card article 11 are respectively 70mm, 5mm and 6mm, the length and width and thickness of the first card article 12
Respectively 70mm, 15mm and 6mm, the length and width of beehive body 5 and high are respectively 495mm, 80mm and 270mm, lucite case lid
1 length and width is 495mm and 80mm, and the second card article 11 is 15mm with the edge distance of the opening of beehive body 5.
All around face is lucite material and fixed border, beehive sheet with lower shoe to visual beehive body 5
Body 5 is smooth open type opening, is stamped two-layer cover plate, rustless steel web plate 6 of the ground floor for hollow out, beneficial to ventilation, the superiors
For lucite case lid 1, cover and wear a logical nitrogen pore 3, conveniently enter nitrogen, edges of boards edge is stained with single side self sponge strip, slightly towards
Lower applying power, can ensure the good sealing of beehive, the second card article 11 and the lucite case lid 1 at the top of beehive body 5
Junction is tightly connected by single side self sponge strip, is blocked on the first card article 12 inside lucite case lid 1 and beehive body 5
Rustless steel web plate 6 is connected to, on the lucite case lid 1 of 2 side of handle, logical nitrogen pore 3 is provided with（Less than the hole of passage 10
It is many, and it is less than Apiss volume）, when up leading to nitrogen, air can be extruded away from this aperture.
It is a kind of to hatch the method that beehive carries out young honeybee hatching and collection using visual Apiss, comprise the steps：Step one,
Place honeybee spleen；Step 2, the hatching of honeybee spleen；Step 3, cooling；Step 4, clears up dead Apiss；Step 5, anaesthetizes Apiss；Step
Six, Apiss are collected；It is characterized in that：
In step one, the bee variety spleen that will go out room is put in visualization Apiss incubator, rustless steel Net plate cover is covered；
In step 2, the visualization Apiss incubator with the bee variety spleen that will go out room is put in couveuse and is incubated
Change, incubation temperature is 30-34 DEG C, and humidity is 70-90%;
In step 3, Apiss hatch latter first day to the 4th day temperature and reduce by 1 DEG C all in original temperature, relative humidity
It is constant；
In step 4, the 4th or the 5th day after Apiss hatch, honeybee spleen is gently released, the dead Apiss dropped in bottom
Pour out（This step suggestion is carried out under dark or light weak condition）, covered
After machine glass case lid；
In step 5, nitrogen is passed through from the aperture of honeybee lid carries out anesthesia and the collection work of Apiss, wherein nitrogen injection
Flow is 10-15L/min, and the persistent period is 60s ~ 120s；
In step 6, postanesthetic Apiss are collected.
Preferably, in the step 2, incubation temperature is 33 ± 1 DEG C；Humidity is 80 ± 5%.
Preferably, in the step 5, the flow of nitrogen injection is 12L/min；Persistent period is 100s.
It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned one exemplary embodiment, Er Qie
In the case of spirit or essential attributes without departing substantially from the present invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, embodiment all should be regarded as exemplary, and be nonrestrictive, the scope of the present invention is by appended power
Profit is required rather than described above is limited, it is intended that all in the implication and scope of the equivalency of claim by falling
Change is included in the present invention.Any reference in claim should not be considered as and limit involved claim.
Claims (9)
1. a kind of visual Apiss hatch beehive, including beehive body（5）：It is characterized in that：The beehive body（5）Top be
Open type open-type, the beehive body（5）Handle panel is installed with the outer wall of both sides（4）, the beehive body（5）'s
Second card article at top（11）With lucite case lid（1）Junction be tightly connected by single side self sponge strip, the honeybee
Case body（5）The second card article is fixed with open-topped inwall（11）With the first card article（12）, the second card article（11）Positioned at
One card article（12）Top, the lucite case lid（1）Top be fixed with handle（2）, and handle（2）Organic glass of side
Glass case lid（1）On be provided with logical nitrogen pore（3）And passage（10）, the lucite case lid（1）With beehive body（5）It is internal
The first card article（12）On be connected with rustless steel web plate（6）, the rustless steel web plate（6）It is by two pieces of left side plates（7）, two pieces
Side plate in front and back（8）Constitute with stainless steel silk, and handle is installed on stainless steel silk（9）.
2. it is a kind of to hatch the method that beehive carries out young honeybee hatching and collection using visual Apiss, comprise the steps：Step one, puts
Put honeybee spleen；Step 2, the hatching of honeybee spleen；Step 3, cooling；Step 4, clears up dead Apiss；Step 5, anaesthetizes Apiss；Step 6,
Apiss are collected；It is characterized in that：
S1., in step one, the bee variety spleen that will go out room is put in visualization Apiss incubator, rustless steel web plate is covered
Lid；
S2., in step 2, the visualization Apiss incubator with the bee variety spleen that will go out room is put in couveuse to be carried out
Hatching, incubation temperature are 30-34 DEG C, and humidity is 70-90%;
S3., in step 3, Apiss hatch latter first day to the 4th day temperature and reduce by 1 DEG C all in original temperature, relatively
Humidity is constant；
S4. in step 4, the 4th or the 5th day after Apiss hatch, honeybee spleen is gently released, dropping in the dead of bottom
Apiss are poured out, after covering lucite case lid；
S5., in step 5, nitrogen is passed through from the aperture of honeybee lid carries out anesthesia and the collection work of Apiss, wherein nitrogen injection
Flow be 10-15L/min, the persistent period be 60s ~ 120s；
S6., in step 6, postanesthetic Apiss are collected.
3. a kind of visual Apiss according to claim 1 hatch beehive, it is characterised in that：The lucite case lid（1）
With beehive body（5）It is made up of the component of lucite material, and beehive body（5）The all around junction with lower shoe
It is bonded with single side self sponge strip.
4. a kind of visual Apiss according to claim 1 hatch beehive, it is characterised in that：The logical nitrogen pore（3）Hole
Footpath is more than passage（10）Aperture, and logical nitrogen pore（3）Aperture be 10mm.
5. a kind of visual Apiss according to claim 1 hatch beehive, it is characterised in that：Second card article（11）'s
Length and width and thickness are respectively 70mm, 5mm and 6mm, the first card article（12）Length and width and thickness be respectively 70mm, 15mm and 6mm.
6. a kind of visual Apiss according to claim 1 hatch beehive, it is characterised in that：The beehive body（5）Length,
Wide and high respectively 495mm, 80mm and 270mm, lucite case lid（1）Length and width be 495mm and 80mm.
7. a kind of visual Apiss according to claim 1 hatch beehive, it is characterised in that：Second card article（11）With honeybee
Case body（5）Opening edge distance be 15mm.
8. it is according to claim 2 it is a kind of hatch the beehive method that carries out young honeybee hatching and collection using visual Apiss, its
It is characterised by：In the step 2, incubation temperature is 33 ± 1 DEG C；Humidity is 80 ± 5%.
9. it is according to claim 2 it is a kind of hatch the beehive method that carries out young honeybee hatching and collection using visual Apiss, its
It is characterised by：In the step 5, the flow of nitrogen injection is 12L/min；Persistent period is 100s.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611221243.3A CN106614107A (en) | 2016-12-27 | 2016-12-27 | Method for performing bee larva incubation and collection by means of visualized bee incubation beehive |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611221243.3A CN106614107A (en) | 2016-12-27 | 2016-12-27 | Method for performing bee larva incubation and collection by means of visualized bee incubation beehive |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106614107A true CN106614107A (en) | 2017-05-10 |
Family
ID=58831351
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611221243.3A Pending CN106614107A (en) | 2016-12-27 | 2016-12-27 | Method for performing bee larva incubation and collection by means of visualized bee incubation beehive |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106614107A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108739680A (en) * | 2018-05-29 | 2018-11-06 | 中国农业科学院蜜蜂研究所 | A kind of test method for measuring Pesticides on Honeybee larvae development and influencing |
| CN111248158A (en) * | 2020-03-17 | 2020-06-09 | 生态环境部南京环境科学研究所 | Automatic bee collecting device |
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| GB138448A (en) * | 1919-02-26 | 1920-02-12 | Ahmed Zaky Abushady | Improved means for artificially hatching queen bees |
| US1388085A (en) * | 1920-09-30 | 1921-08-16 | Eugene B Ault | Bee-shipping cage |
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| CN204968921U (en) * | 2015-07-28 | 2016-01-20 | 中国计量学院 | A beehive that is arranged in breed of indoor bee to observe |
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2016
- 2016-12-27 CN CN201611221243.3A patent/CN106614107A/en active Pending
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| GB138448A (en) * | 1919-02-26 | 1920-02-12 | Ahmed Zaky Abushady | Improved means for artificially hatching queen bees |
| US1388085A (en) * | 1920-09-30 | 1921-08-16 | Eugene B Ault | Bee-shipping cage |
| CN101720680A (en) * | 2008-10-27 | 2010-06-09 | 郑斌 | Novel hive |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108739680A (en) * | 2018-05-29 | 2018-11-06 | 中国农业科学院蜜蜂研究所 | A kind of test method for measuring Pesticides on Honeybee larvae development and influencing |
| CN111248158A (en) * | 2020-03-17 | 2020-06-09 | 生态环境部南京环境科学研究所 | Automatic bee collecting device |
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Application publication date: 20170510 |
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| RJ01 | Rejection of invention patent application after publication |