CN102640734A - Method for carrying out bee colony anaesthesia by utilizing bee honeycomb anaesthesia device - Google Patents
Method for carrying out bee colony anaesthesia by utilizing bee honeycomb anaesthesia device Download PDFInfo
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- CN102640734A CN102640734A CN2012101549820A CN201210154982A CN102640734A CN 102640734 A CN102640734 A CN 102640734A CN 2012101549820 A CN2012101549820 A CN 2012101549820A CN 201210154982 A CN201210154982 A CN 201210154982A CN 102640734 A CN102640734 A CN 102640734A
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Abstract
The invention discloses a method for carrying out bee colony anaesthesia by utilizing a bee honeycomb anaesthesia device. The method comprises the following steps of: taking a honeycomb from a bee box, and placing the honeycomb in a honeycomb groove so as to place in a box body; introducing carbon dioxide in the box body through a guide tube, exhausting air in the box body, and moving a collecting tank and the honeycomb groove into the box body completely, wherein the honeycomb groove is subjected to local anaesthesia through carbon dioxide introduced in the box body; and stopping introducing the carbon dioxide after bees on the honeycomb are in full anaesthesia, shivering the bees to the collecting tank, and taking out anesthetic bees so as to be used for a bee biologic test. The method provided by the invention overcomes the defects that the number of the anesthetic living bodies is small and the pollution is easy to generate in the conventional method; the method has the characteristics that anaesthesia on bees can be carried out safely in batches, the anaesthesia quantity is large, the processing cost is low, the operability is strong and the like; and the method is suitable for a zoology poisonousness testing test of a great deal of bees, and is a preparing method of a zoology poisonousness testing test bee living body which is high efficiency, safe and economical.
Description
Technical field
The invention belongs to the eco-toxicity field tests, the bee variety spleen colony anesthesia that is specifically related to a kind of improved bee variety spleen anesthesia test device and utilizes this device to carry out.
Background technology
In the observational study process of honeybee kind activity insect; Often need carry out in-plant operation to it; Honeybee anaesthetized etc. will help later stage carrying out of operating process closely after the pre-treatment process greatly, present adoptable anesthesia mainly contains etherization method and carbon dioxide narcosis method.Adopt the etherization method that activity insects such as honeybee are anaesthetized; Need the operator to have good operating experience, and the usage amount of strict control ether, and ether is prone to tested insect is caused certain injury; Influence the later stage accuracy of experimental results; Too much use ether also can pollute the laboratory indoor environment, has influence on the healthy of testing crew.Therefore the present trend that adopts carbon dioxide narcosis to become the insect anesthesia, but present more existing carbon dioxide narcosis devices are because the anesthesia outfit that is mostly low capacity that adopts; Before the anesthesia, need carry out the transfer of honeybee, this process often wastes time and energy; Testing crew also is subject to honeybee and bites and cause personal injury, as when carrying out test and need anaesthetize honeybee in batches, whole anesthesia process will take time and effort; Increase experimentation cost greatly, and slow down the test process; And more existing anesthesia outfit complex structures, use loaded down with trivial details, anesthesia workload big.Therefore, when carrying out test such as the ecological toxicity scale of honeybee test, a kind of simple to operation, cost is low, environmental pollution is little, anaesthetic effect is good anesthesia is provided, also becomes the focus of paying close attention in this research field.
Summary of the invention
The objective of the invention is to the material toxicity that exists in the present honeybee anesthesia big; Environmental hazard is high; Take time and effort, the anesthesia test cost increases, and process of the test is subject to slow down; Problems such as testing crew health is vulnerable provide a kind of method of utilizing bee variety spleen anesthesia outfit to carry out bee colony anesthesia.
Another object of the present invention provides a kind of bee variety spleen anesthesia outfit that is applied to above-mentioned bee colony anesthesia.
The object of the invention can reach through following measure:
A kind of method of utilizing bee variety spleen anesthesia outfit to carry out bee colony anesthesia, it may further comprise the steps:
A, the honeybee spleen that will from beehive, take out are put into the honeybee spleen groove of bee variety spleen anesthesia outfit, and place in the casing of bee variety spleen anesthesia outfit;
B, in the last direction casing of bee variety spleen anesthesia outfit casing, feed carbon dioxide; Discharge the air in this casing; Fully move into casing in the honeybee spleen groove that the honeybee spleen is housed the feeder of bee variety spleen anesthesia outfit; The carbonic acid gas that feeds in the casing carries out local paralysis to honeybee spleen groove, and flows out casing through the gap between honeybee spleen groove sealing surface and honeybee feeder sealing surface and the casing;
C, treat that honeybee on the honeybee spleen all after the anesthesia, stops to feed carbon dioxide, honeybee is trembled in the feeder, take out the honeybee of anesthesia.
The invention also discloses a kind of bee variety spleen anesthesia outfit; It is the bee variety spleen anesthesia outfit that is adopted in the above-mentioned anesthesia; It comprises carbonic acid gas feeding mechanism, conduit and casing; One end of said conduit connects the gas outlet of carbonic acid gas feeding mechanism, and the other end of conduit connects the top or the side wall upper part of said casing; The inside of its said casing is provided with honeybee spleen groove and honeybee feeder; The honeybee feeder is positioned at the below of said honeybee spleen groove; Said honeybee spleen groove and honeybee feeder flexibly connect with casing respectively; Be provided with honeybee spleen groove sealing surface in the outer end of honeybee spleen groove; Be provided with honeybee feeder sealing surface in the outer end of honeybee feeder, when honeybee spleen groove and honeybee feeder moved in the casing fully, the carbonic acid gas that feeds in the casing only flowed out casing through the gap between honeybee spleen groove sealing surface and honeybee feeder sealing surface and the casing.
The honeybee of being said among the present invention is mainly the honeybee of artificial feeding, and honeybee grows in beehive, and the honeybee spleen of the solid of or strip and block square by polylith is formed in the beehive.
Casing whole or at least one side adopt transparent lucite to process, preferably adopt transparent lucite to process on the whole.The general employing of casing is square, and the width of casing matees the width of one or more honeybee spleens.When only establishing a honeybee spleen groove and honeybee feeder in the casing, preferred 40 ~ 50 centimetres of casing length, preferred 20 ~ 30 centimetres of width, with the honeybee spleen width coupling of present bee raising, highly preferred 30 ~ 40 centimetres, with honeybee spleen groove and feeder stack thickness coupling.
Also can be provided with a plurality of honeybee spleen grooves and honeybee feeder in the casing, when honeybee spleen groove is a plurality of, but file, line or mode that both mix are arranged; When the honeybee feeder is a plurality of, can under each honeybee spleen groove, all establish a honeybee feeder, also can establish a plurality of honeybee feeders side by side in the bottom of casing.
Between needing between honeybee feeder and the honeybee spleen groove, be separated with ventilation space.Honeybee spleen groove is used for the static honeybee spleen of placing to be waited to anaesthetize, and the honeybee feeder treats that in order to collect postanesthetic honeybee further biologic test is required.Flexible connection between honeybee feeder and honeybee spleen groove and the casing can realize that the honeybee feeder also can directly slide through the chute that is located on box house or the inwall on bottom half.Honeybee feeder and honeybee spleen groove can also directly take out in casing, are beneficial to place and take out the carrying out of operation.
Generally greater than the length and/or the width in (being honeybee spleen groove sealing surface) with it parallel honeybee spleen groove cell body cross section, its length and width also can be slightly less than the length and/or the width in cell body cross section, are preferably greater than for the length of honeybee spleen groove sealing surface and width; The length of honeybee feeder sealing surface and width are generally greater than the length and/or the width in (being honeybee feeder sealing surface) with it parallel honeybee feeder cell body cross section; Its length and width also can be slightly less than the length and/or the width in cell body cross section, are preferably greater than.The sealing surface that honeybee spleen groove and honeybee feeder move into behind the casing fully is used to discharge the original air in the casing with gap between the casing or passage, and slows down air entering box house, simultaneously carbonic acid gas is derived outside the casing.This set can make the carbonic acid gas that feeds in the casing flow out casing through the gap between honeybee spleen groove sealing surface and honeybee feeder sealing surface and the casing.
At place, the gas outlet of said carbonic acid gas feeding mechanism or on said conduit, be provided with the ventilation switch, preferably conduit near the casing place this switch is set, with the input of control gas.The carbonic acid gas feeding mechanism can also can generally can adopt dioxide bottle for storing the device of carbonic acid gas for directly producing the device of carbonic acid gas.Place, gas outlet at said carbonic acid gas feeding mechanism also is provided with the gas pressure control device, and it can be air pressure reducer; Regulate output gas pressure through the gas pressure control device, generally output pressure is controlled at 0.01～0.5MPa, preferred 0.1MPa.In the bee colony anesthesia; After feeder moves into casing fully with the honeybee spleen groove that the honeybee spleen is housed; The feeding time of carbon dioxide generally is adjusted into 2～20min; Preferred 5～15min, wherein when 10min (under the 0.1MPa condition), can reach does not have the work such as contamination or observation that tangible murder by poisoning and anesthesia duration do not exceed general toxotest basically to test or the anesthesia honeybee that detects usefulness.
Operating process of the present invention can be carried out at ambient temperature.
The present invention adopts bee variety spleen anesthesia outfit, can bee variety spleen integral body be positioned in the non-complete sealing device, need not honeybee is carried out disturbance; Also need not single seizure and shift honeybee, in device, feed carbonic acid gas afterwards, control feeds the flow velocity and the anesthesia duration of carbonic acid gas; And recovery time in later stage etc. parameter, carry out the group anesthesia of bee variety spleen in batches, after the whole anesthesia of honeybee; The honeybee of anesthesia is trembled into feeder; Accomplish the whole collection of honeybee, supply step test down required, test and other tests or detection especially for the eco-toxicity of batch honeybee.
The bee variety spleen anesthesia outfit that adopts among the present invention, material is lucite, carbon dioxide steel cylinder and breather pipe etc., and material is easy to get, and cost is lower.It is simple to operation that the bee variety spleen anesthesia test device that adopts carries out the method for honeybee anesthesia; Cost is low; Environmental pollution is little; Anaesthetic effect is good, and the early stage that is applicable to the test that bee biological body demand such as honeybee eco-toxicity testing experiment is bigger is the set-up procedure of honeybee in batches, is a kind of economical and practical batch honeybee anesthesia.Adopt when of the present invention; The flow velocity and the time of the carbonic acid gas of controlling well ventilation; Controlledly make stable honeybee recovery time, and can stablize and obtain anesthesia honeybee in batches, the vigor of anesthesia honeybee does not have significant change; Can effectively accelerate the enforcement progress of honeybee eco-toxicity test, improve the ease for operation of test.
Description of drawings
Fig. 1 is a bee variety spleen anesthesia outfit structural representation of the present invention.
Among the figure: 1, dioxide bottle, 2, pressure control device, 3, wireway, 4, the ventilation switch, 5, casing, 6, honeybee spleen groove, 7, feeder, 8, honeybee spleen groove sealing surface, 9, the feeder sealing surface.
Embodiment
Below embodiment through accompanying drawing the present invention is further specified.
A kind of structure of bee variety spleen anesthesia outfit is as shown in Figure 1, and it comprises dioxide bottle, wireway, casing, honeybee spleen groove and feeder, and the gas outlet of dioxide bottle is provided with pressure control device, regulates the CO of output
2Pressure, an end of conduit connects the gas outlet of carbonic acid gas feeding mechanism, the top or the side wall upper part of the other end connect box of conduit, the ventilation switch be arranged on conduit near the casing place.
Casing adopts transparent lucite to process, for square, and the width of its width coupling honeybee spleen.Honeybee spleen groove and honeybee feeder are movably connected on box house, and honeybee spleen groove is last, and the honeybee feeder leaves suitable ventilation space down between the two.Be provided with honeybee spleen groove sealing surface in the outer end of honeybee spleen groove; Be provided with honeybee feeder sealing surface in the outer end of honeybee feeder; When honeybee spleen groove and honeybee feeder moved in the casing fully, the carbonic acid gas that feeds in the casing only flowed out casing through the gap between honeybee spleen groove sealing surface and honeybee feeder sealing surface and the casing.
The concrete grammar that utilizes above-mentioned bee variety spleen anesthesia outfit to carry out bee colony anesthesia in following each example is: the honeybee spleen that will from beehive, take out is earlier put into honeybee spleen groove, and places in the casing; In the last direction casing of casing, feed carbon dioxide through conduit again; Discharge the air in this casing; Move into feeder and the honeybee spleen groove that the honeybee spleen is housed in the casing fully; The carbonic acid gas that feeds in the casing carries out local paralysis to honeybee spleen groove, and flows out casing through the gap between honeybee spleen groove sealing surface and honeybee feeder sealing surface and the casing; The CO that control feeds
2Pressure condition and feeding time, treat that honeybee on the honeybee spleen all after the anesthesia, stops to feed carbon dioxide, honeybee is trembled in the feeder, take out the honeybee of anesthesia, the bee biological test is used after treating.
Embodiment 1:
The environmental condition of honeybee anesthesia: indoor temperature: 25 ℃; The CO that feeds
2Pressure: 0.1Mpa all amounts to 117 honeybees and waits to anaesthetize.Continue to feed CO
2Behind the 5min, stop to feed CO
2, after the anesthesia honeybee recovered through 5min, recovery rate was 3.41%; Recovery rate is 71.8% behind the 8min; Recovery rate is 100% behind the 10min.
Embodiment 2:
Adopt the environmental condition of honeybee anesthesia in the instance 1 and the CO of feeding
2Pressure condition all amounts to 115 honeybees and waits to anaesthetize.Logical CO
2Behind the 10min, stop to feed CO
2, after the anesthesia honeybee recovered through 5min, recovery rate was 0%; Recovery rate is 1.74% behind the 10min; Recovery rate is 49.6% behind the 15min; Recovery rate is 98.3% behind the 20min, and recovery rate is 100% behind the 25min.
Embodiment 3:
Adopt the environmental condition of honeybee anesthesia in the instance 1 and the CO of feeding
2Pressure condition all amounts to 128 honeybees and waits to anaesthetize.Logical CO
2Behind the 15min, stop to feed CO
2, after the anesthesia honeybee recovered through 5min, recovery rate was 0%; Recovery rate is 0.86% behind the 10min; Recovery rate is 23.5% behind the 15min; Recovery rate is 86.4% behind the 20min, and recovery rate is 99.6% behind the 25min.
Embodiment 4:
According to honeybee agricultural chemicals eco-toxicity testing standard method of operating and corresponding standard operational procedure, adopt Rogor as its acute toxicity ability of standard pesticide research, to not carrying out CO to honeybee
2The honeybee of anesthesia carries out toxotest, obtains the 24h acute toxicity LC of agricultural chemicals Rogor to honeybee
50Be 0.29 μ g/ honeybee (confidence limit is 0.22～0.39 μ g/ honeybee), 48h acute toxicity LC
50Be 0.21 μ g/ honeybee (confidence limit is 0.16～0.28 μ g/ honeybee).
Embodiment 5:
Rogor in the employing instance 4 is to the acute toxic test method of honeybee, and the batch honeybee that anesthesia back in the instance 1 is recovered carries out agricultural chemicals Rogor acute toxic test, obtains the 24h acute toxicity LC of Rogor to this batch honeybee
50Be 0.27 μ g/ honeybee (confidence limit is 0.21～0.35 μ g/ honeybee), 48h acute toxicity LC
50Be 0.18 μ g/ honeybee (confidence limit is 0.13～0.23 μ g/ honeybee).
Embodiment 6:
Rogor in the employing instance 4 is to the acute toxic test method of honeybee, and the batch honeybee that anesthesia back in the instance 2 is recovered carries out agricultural chemicals Rogor acute toxic test, obtains the 24h acute toxicity LC of Rogor to this batch honeybee
50Be 0.22 μ g/ honeybee (confidence limit is 0.18～0.28 μ g/ honeybee), 48h acute toxicity LC
50Be 0.15 μ g/ honeybee (confidence limit is 0.11～0.19 μ g/ honeybee).
Embodiment 7:
Rogor in the employing instance 4 is to the acute toxic test method of honeybee, and the batch honeybee that anesthesia back in the instance 3 is recovered carries out agricultural chemicals Rogor acute toxic test, obtains the 24h acute toxicity LC of Rogor to this batch honeybee
50Be 0.20 μ g/ honeybee (confidence limit is 0.16～0.25 μ g/ honeybee), 48h acute toxicity LC
50Be 0.12 μ g/ honeybee (confidence limit is 0.08～0.15 μ g/ honeybee).
Safety analysis
The method that adopts the device among the present invention to carry out honeybee anesthesia; The carbonic acid gas that adopts in the anesthesia process does not have harm to environment and testing crew; Optimum control carbonic acid gas duration of ventilation 10min (under the 0.1Mpa pressure condition); Anesthesia honeybee to test usefulness does not have tangible murder by poisoning basically, and the eco-toxicity result of the honeybee test that the later stage is carried out is not significantly influence also.Postanesthetic recovery time preferred 10min; In 10min, can accomplish the work such as contamination or observation of honeybee eco-toxicity test; Also can avoid testing crew is produced unnecessary honeybee bite injury and other loaded down with trivial details situation; Therefore, adopt improved bee variety spleen anesthesia test device carry out bee colony anesthesia method, be a kind of environmental risk lower, easy to operate, to the less reliable method of honeybee influence.
Claims (10)
1. one kind is utilized bee variety spleen anesthesia outfit to carry out the method that bee colony is anaesthetized, and it is characterized in that may further comprise the steps:
A, the honeybee spleen that will from beehive, take out are put into honeybee spleen groove, and place in the casing of bee variety spleen anesthesia outfit;
B, in the last direction casing of bee variety spleen anesthesia outfit casing, feed carbon dioxide; Discharge the air in this casing; Move into feeder and the honeybee spleen groove that the honeybee spleen is housed in the casing fully; The carbonic acid gas that feeds in the casing carries out local paralysis to honeybee spleen groove, and flows out casing through the gap between honeybee spleen groove sealing surface and honeybee feeder sealing surface and the casing;
C, treat that honeybee on the honeybee spleen all after the anesthesia, stops to feed carbon dioxide, honeybee is trembled in the feeder, take out the honeybee of anesthesia;
Wherein said bee variety spleen anesthesia outfit comprises carbonic acid gas feeding mechanism, conduit and casing, and an end of said conduit connects the gas outlet of carbonic acid gas feeding mechanism, and the other end of conduit connects the top or the side wall upper part of said casing; Be provided with honeybee spleen groove and honeybee feeder in the inside of said casing; The honeybee feeder is positioned at the below of said honeybee spleen groove; Said honeybee spleen groove and honeybee feeder flexibly connect with casing respectively; Be provided with honeybee spleen groove sealing surface in the outer end of honeybee spleen groove; Be provided with honeybee feeder sealing surface in the outer end of honeybee feeder, when honeybee spleen groove and honeybee feeder moved in the casing fully, the carbonic acid gas that feeds in the casing only flowed out casing through the gap between honeybee spleen groove sealing surface and honeybee feeder sealing surface and the casing.
2. method according to claim 1, it is characterized in that feeder and the honeybee spleen groove that the honeybee spleen is housed move into casing fully after, the feeding time of carbon dioxide is 2～20min.
3. method according to claim 1, the pressure that it is characterized in that feeding the carbon dioxide in the bee variety spleen anesthesia outfit casing is 0.1MPa.
4. method according to claim 1 is characterized in that the whole of said casing or at least simultaneously adopts transparent lucite to process.
5. method according to claim 1 is separated with ventilation space between it is characterized in that between said honeybee feeder and the honeybee spleen groove; The length of said honeybee spleen groove sealing surface and length and/or the width of width greater than parallel with it honeybee spleen groove cell body cross section; The length of said honeybee feeder sealing surface and length and/or the width of width greater than parallel with it honeybee feeder cell body cross section.
6. method according to claim 1 is characterized in that perhaps on said conduit, being provided with the ventilation switch at the place, gas outlet of said carbonic acid gas feeding mechanism, is provided with the gas pressure control device at the place, gas outlet of said carbonic acid gas feeding mechanism.
7. bee variety spleen anesthesia outfit; Comprise carbonic acid gas feeding mechanism, conduit and casing; One end of said conduit connects the gas outlet of carbonic acid gas feeding mechanism, and the other end of conduit connects the top or the side wall upper part of said casing, it is characterized in that being provided with in the inside of said casing honeybee spleen groove and honeybee feeder; The honeybee feeder is positioned at the below of said honeybee spleen groove; Said honeybee spleen groove and honeybee feeder flexibly connect with casing respectively, are provided with honeybee spleen groove sealing surface in the outer end of honeybee spleen groove, are provided with honeybee feeder sealing surface in the outer end of honeybee feeder; When honeybee spleen groove and honeybee feeder moved in the casing fully, the carbonic acid gas that feeds in the casing only flowed out casing through the gap between honeybee spleen groove sealing surface and honeybee feeder sealing surface and the casing.
8. bee variety spleen anesthesia outfit according to claim 7 is characterized in that the whole of said casing or at least simultaneously adopts transparent lucite to process.
9. bee variety spleen anesthesia outfit according to claim 7 is separated with ventilation space between it is characterized in that between said honeybee feeder and the honeybee spleen groove; The length of said honeybee spleen groove sealing surface and length and/or the width of width greater than parallel with it honeybee spleen groove cell body cross section; The length of said honeybee feeder sealing surface and length and/or the width of width greater than parallel with it honeybee feeder cell body cross section.
10. bee variety spleen anesthesia outfit according to claim 7; It is characterized in that perhaps on said conduit, being provided with the ventilation switch, be provided with the gas pressure control device at the place, gas outlet of said carbonic acid gas feeding mechanism at the place, gas outlet of said carbonic acid gas feeding mechanism.
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| CN106172253A (en) * | 2016-08-01 | 2016-12-07 | 山东省农业科学院植物保护研究所 | A kind of Orius batch production collection method and collection device |
| CN106212391A (en) * | 2016-08-01 | 2016-12-14 | 山东省农业科学院植物保护研究所 | The collection method of a kind of factorial praluction live body Phyloseiulus nersimilis and device |
| CN106614107A (en) * | 2016-12-27 | 2017-05-10 | 广东中科英海科技有限公司 | Method for performing bee larva incubation and collection by means of visualized bee incubation beehive |
| CN108566903A (en) * | 2017-03-13 | 2018-09-25 | 上海市农药研究所有限公司 | Honeybee acute oral toxicity test device |
| CN115152702A (en) * | 2022-06-23 | 2022-10-11 | 安徽华辰检测技术研究院有限公司 | Bee acute oral toxicity test and acute contact toxicity test method |
| CN115399294A (en) * | 2022-07-21 | 2022-11-29 | 扬州大学 | Dynamic determination method for liriomyza trifoliata imago CTmax |
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| CN106172253A (en) * | 2016-08-01 | 2016-12-07 | 山东省农业科学院植物保护研究所 | A kind of Orius batch production collection method and collection device |
| CN106212391A (en) * | 2016-08-01 | 2016-12-14 | 山东省农业科学院植物保护研究所 | The collection method of a kind of factorial praluction live body Phyloseiulus nersimilis and device |
| CN106172253B (en) * | 2016-08-01 | 2019-03-29 | 山东省农业科学院植物保护研究所 | A kind of Orius batch production collection method and collection device |
| CN106212391B (en) * | 2016-08-01 | 2020-07-17 | 山东省农业科学院植物保护研究所 | Collection method and device for industrially producing living phytoseiulus persimilis |
| CN106614107A (en) * | 2016-12-27 | 2017-05-10 | 广东中科英海科技有限公司 | Method for performing bee larva incubation and collection by means of visualized bee incubation beehive |
| CN108566903A (en) * | 2017-03-13 | 2018-09-25 | 上海市农药研究所有限公司 | Honeybee acute oral toxicity test device |
| CN115152702A (en) * | 2022-06-23 | 2022-10-11 | 安徽华辰检测技术研究院有限公司 | Bee acute oral toxicity test and acute contact toxicity test method |
| CN115152702B (en) * | 2022-06-23 | 2023-11-03 | 安徽华辰检测技术研究院有限公司 | Bee acute oral toxicity test and acute contact toxicity test method |
| CN115399294A (en) * | 2022-07-21 | 2022-11-29 | 扬州大学 | Dynamic determination method for liriomyza trifoliata imago CTmax |
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