CN115152702B - Bee acute oral toxicity test and acute contact toxicity test method - Google Patents
Bee acute oral toxicity test and acute contact toxicity test method Download PDFInfo
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- CN115152702B CN115152702B CN202210716618.2A CN202210716618A CN115152702B CN 115152702 B CN115152702 B CN 115152702B CN 202210716618 A CN202210716618 A CN 202210716618A CN 115152702 B CN115152702 B CN 115152702B
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- 238000012360 testing method Methods 0.000 title claims abstract description 41
- 231100000460 acute oral toxicity Toxicity 0.000 title claims abstract description 24
- 230000001154 acute effect Effects 0.000 title claims abstract description 20
- 231100000820 toxicity test Toxicity 0.000 title claims abstract description 18
- 238000010998 test method Methods 0.000 title claims abstract description 11
- 239000007788 liquid Substances 0.000 claims abstract description 121
- 241000257303 Hymenoptera Species 0.000 claims abstract description 61
- 239000003814 drug Substances 0.000 claims abstract description 27
- 238000000034 method Methods 0.000 claims abstract description 14
- 235000003642 hunger Nutrition 0.000 claims abstract description 9
- 206010002091 Anaesthesia Diseases 0.000 claims abstract description 7
- 230000037005 anaesthesia Effects 0.000 claims abstract description 7
- 230000037351 starvation Effects 0.000 claims abstract description 7
- 230000000903 blocking effect Effects 0.000 claims description 62
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 12
- 229930006000 Sucrose Natural products 0.000 claims description 12
- 239000005720 sucrose Substances 0.000 claims description 12
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 10
- 238000005303 weighing Methods 0.000 claims description 7
- 229910002092 carbon dioxide Inorganic materials 0.000 claims description 5
- 239000001569 carbon dioxide Substances 0.000 claims description 5
- 230000000384 rearing effect Effects 0.000 claims description 5
- 239000002904 solvent Substances 0.000 claims description 4
- 231100000419 toxicity Toxicity 0.000 claims 2
- 230000001988 toxicity Effects 0.000 claims 2
- 239000011148 porous material Substances 0.000 claims 1
- 230000008569 process Effects 0.000 abstract description 6
- 238000013417 toxicology model Methods 0.000 abstract 1
- 241000256844 Apis mellifera Species 0.000 description 26
- 230000004308 accommodation Effects 0.000 description 4
- 235000005911 diet Nutrition 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 241000256837 Apidae Species 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 238000004891 communication Methods 0.000 description 2
- 230000037213 diet Effects 0.000 description 2
- 230000000378 dietary effect Effects 0.000 description 2
- 238000007599 discharging Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 231100000215 acute (single dose) toxicity testing Toxicity 0.000 description 1
- 238000011047 acute toxicity test Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000004247 hand Anatomy 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 239000011344 liquid material Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 238000004080 punching Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000012502 risk assessment Methods 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 210000003813 thumb Anatomy 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K53/00—Feeding or drinking appliances for bees
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Infusion, Injection, And Reservoir Apparatuses (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a bee acute oral toxicity test and an acute contact toxicity test method, which are characterized in that starvation treatment is carried out on bees after the bees are collected, the starvation treatment bees directly carry out the bee acute oral toxicity test and the acute contact toxicity test, and the bees are fed through a feeder in the experimental process, so that the bees are prevented from being hurt, the bee acute oral toxicity model can be opened without anesthesia, the feeder solves the problem of incomplete feeding of liquid medicine, and meanwhile, the dripping of the liquid medicine is avoided.
Description
Technical Field
The invention relates to the technical field of bee experiment methods, in particular to a bee acute oral toxicity test and an acute contact toxicity test method.
Background
Acute oral toxicity test of bees: the test substances with different dosages are dispersed in the sucrose solution to feed adult worker bees, the consumption of the liquid medicine is measured, and the sucrose solution without the test substances is fed after the liquid medicine is consumed.
Bee acute contact toxicity test: after the bees are anesthetized, the test liquid medicine with different concentrations is dripped at the middle chest backboard of the test bees, after the solvent volatilizes, the bees are transferred into a test cage, and a proper amount of sucrose water is soaked in absorbent cotton for feeding.
Traditional bee acute toxicity test bee collection requires spleen-regulating anesthesia. Then transferring every 10 bees into 1 bee cage, and carrying out anesthesia on the awakened bees for two or more times, which can cause great damage to the bees. The acute oral toxicity test of honeybees uses centrifuging tube or syringe, and the centrifuging tube needs to prick the hole by people, and the syringe needs to be pushed by people for a certain time to make the liquid medicine suspend and not fall, and when weighing the feeding tube and when dying the honeybees, there is the risk that the liquid medicine drips, has the honeybee to eat clean drawback simultaneously, and then influences experimental accuracy, and pesticide produces very big influence to the risk assessment of honeybee.
Disclosure of Invention
In order to solve the technical problems in the background technology, the invention provides a bee acute oral toxicity test and an acute contact toxicity test method.
The invention provides a bee acute oral toxicity test and an acute contact toxicity test method, which comprise the following steps:
starvation treatment:
placing a certain number of bees into a rearing cage, and starving for 1-3 hours under the dark condition;
acute oral toxicity test of bees:
placing the starved bees into a feeding box;
weighing the feeder filled with the experimental liquid medicine in the feeder filled with the experimental liquid medicine, putting the weighed feeder into a feeding box, taking out the feeder after 3-6 hours, and weighing the feeder;
after bees in the feeder absorb a certain amount of experimental liquid medicine, the feeder filled with sucrose solution is placed in the feeding box;
bee acute contact toxicity test was performed:
placing starved bees into a centrifuge tube, introducing carbon dioxide into the centrifuge tube for anesthesia for 3-5 s, placing bees on a test bench, clamping wings of the bees by using forceps, dropping 1 mu L of test liquid medicine to a middle chest backboard of the bees by using a micro-dropper or a pipette, and transferring the bees into a bee box after the solvent volatilizes;
a feeder containing a sucrose solution is placed in the feeder box.
The feeder can be for can holding the container of liquid material and having the opening be convenient for honeybee diet among the prior art, and specifically, the feeder includes the holding column, open on the top of holding column has the holding tank, and the opening of holding tank is more convenient for honeybee diet feeder liquid in the holding tank of lateral part at the relative holding tank of holding tank opening in the top of holding column.
Preferably, the holding groove is columnar, the aperture of the holding groove is 7mm, the volume of the holding groove is slightly larger than 200 mu L, the opening of the holding groove only allows the body of one bee to extend into the holding groove to drink the liquid in the holding groove, so that two or more bees are prevented from simultaneously eating the liquid in the holding groove, and the feeder is prevented from toppling over.
Preferably, still include the panel, open on the panel has the accommodation hole, hold the vertical slidable mounting of post in the accommodation hole, avoid holding the post and empty when feeding the box outside, specific, the panel can be for can opening among the prior art to have the panel that has certain thickness of accommodation hole, avoid holding the post and empty.
Preferably, the liquid blocking device further comprises a liquid blocking ring, wherein the liquid blocking ring is installed on the accommodating column and used for preventing the solution from splashing out of the accommodating groove, and the liquid blocking ring can be made of plastic or metal or paper materials.
Preferably, the liquid blocking ring is conical, the inner diameter of the liquid blocking ring gradually becomes smaller from one end close to the accommodating groove to one end far away from the accommodating groove, the diameter of one end far away from the accommodating groove of the liquid blocking ring is 1-4mm, namely the aperture of the opening of the liquid blocking ring is 1-4mm, so that liquid in the accommodating groove is prevented from leaking from the feeder in the process of placing the feeder into the feeding box, the conical liquid blocking ring is convenient for placing the liquid on the inner wall of the liquid blocking ring behind the feeder and discharging the liquid into the accommodating groove, the diameter of one end far away from the accommodating groove of the liquid blocking ring is 1-4mm, and the liquid is prevented from being discharged from the opening of the liquid blocking ring under the action of the surface tension of the liquid.
In order to facilitate the detachment of the liquid blocking ring, preferably, the liquid blocking ring is made of thinner paper, the outer side wall of the liquid blocking ring is provided with annular scratches along the circumferential direction of the liquid blocking ring, and when the liquid blocking ring and the feeder are placed in the feeding box together, the liquid blocking ring is broken from the annular scratches by pulling the liquid blocking ring so that the liquid blocking ring part separated from the feeder is taken out of the feeding box.
Preferably, the liquid blocking ring comprises an inner ring and an outer ring, the inner ring is communicated with the accommodating groove, the outer ring is sleeved and fixed on the outer wall of the inner ring, the outer ring is fixed on the outer wall of the accommodating column, the annular scratch is formed on the outer ring, the annular scratch and the upper end face of the accommodating column are in the same plane, the liquid blocking ring is convenient to connect with the feeder, and meanwhile the liquid blocking ring is convenient to separate dietary liquid from the opening of the accommodating groove along the annular scratch.
Preferably, the lower extreme of holding the post is connected with the bottom plate of feeding box through the screw thread mode, prevents on the one hand that the feeder from empting, and on the other hand is convenient for keep off the dismantlement of liquid ring, and is convenient for keep off liquid of liquid ring inner wall and arrange to the holding tank in increase detection precision.
Preferably, the bees in the rearing cage in step S1 are worker bees.
Preferably, the cover of the centrifuge tube in the step S5 is fixedly provided with a pneumatic quick connector, and the pneumatic quick connector is connected with a pneumatic quick structure to introduce carbon dioxide into the centrifuge tube, so that the working efficiency is improved compared with the conventional manual hole punching of the centrifuge tube.
Specifically, in step S3, the test liquid is a mixture of sucrose and the test substance.
According to the acute oral toxicity test and the acute contact toxicity test method for the bees, the bees can be rapidly collected, meanwhile, the damage to the bees is avoided, the acute oral toxicity pattern of the bees can be formed without anaesthetizing the bees, the problem of incomplete feeding of liquid medicine is solved, and meanwhile, the liquid medicine is prevented from dripping.
Additional aspects and advantages of the invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention.
Drawings
FIG. 1 is a schematic diagram of a centrifuge tube in the present method;
FIG. 2 is a schematic diagram of the connection structure of the display board and the feeder of the invention;
FIG. 3 is a schematic view of the structure of the liquid retaining ring of the present invention after being connected to a receiving column;
FIG. 4 is a schematic view of the front plate of the feeder box of the present invention shown in section after the feeder is placed in the feeder box;
fig. 5 is a schematic diagram of the structure of the rear feeder and the feeding box of the invention with the front plate of the feeding box cut away and the broken liquid blocking ring removed.
Detailed Description
Embodiments of the present invention are described in detail below, examples of which are illustrated in the accompanying drawings, wherein like or similar symbols indicate like or similar elements or elements having like or similar functions throughout. The embodiments described below by referring to the drawings are exemplary only for explaining the present invention and are not to be construed as limiting the present invention.
It is to be understood that the terms "center," "longitudinal," "transverse," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counter-clockwise," "axial," "radial," "circumferential," and the like are directional or positional relationships as indicated based on the drawings, merely to facilitate describing the invention and to simplify the description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be configured and operated in a particular orientation, and therefore should not be construed as limiting the invention.
Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include at least one such feature. In the description of the present invention, the meaning of "plurality" means at least two, for example, two, three, etc., unless specifically defined otherwise.
In the present invention, unless explicitly specified and limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally formed; may be mechanically connected, may be electrically connected or may be in communication with each other; either directly or indirectly, through intermediaries, or both, may be in communication with each other or in interaction with each other, unless expressly defined otherwise. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art according to the specific circumstances.
In the present invention, unless expressly stated or limited otherwise, a first feature "up" or "down" a second feature may be the first and second features in direct contact, or the first and second features in indirect contact via an intervening medium. Moreover, a first feature being "above," "over" and "on" a second feature may be a first feature being directly above or obliquely above the second feature, or simply indicating that the first feature is level higher than the second feature. The first feature being "under", "below" and "beneath" the second feature may be the first feature being directly under or obliquely below the second feature, or simply indicating that the first feature is less level than the second feature.
A bee acute oral toxicity test and an acute contact toxicity test method, comprising the following steps:
starvation treatment:
s1: putting on protective clothing, taking the honeycomb out of the beehive, shaking the honeycomb into a bee rearing cage according to the required number of bees after no queen bee is ensured, rapidly closing a zipper of the rearing cage, and carrying out starvation treatment for 2 hours in dark condition;
s2, putting gloves, putting hands into a feeding cage through sleeves of the bee feeding cage, using a 50mL centrifuge tube 3, enabling a tube orifice to cling to the upper part of the feeding cage, buckling bees into the centrifuge tube 3, enabling bees to enter the bottom of the centrifuge tube 3 by slightly shaking the centrifuge tube 3, and after taking 10 bees, pressing the tube orifice with a thumb to prevent the bees from escaping;
acute oral toxicity test of bees:
s300: the centrifuge tube 3 in the S2 is slightly shaken, so that bees enter the bottom of the centrifuge tube 3, the orifice of the centrifuge tube 3 is aligned to the opening of the feeding box 1, and the bees are shaken into the feeding box 1, so that the bees after starvation treatment are placed into the feeding box 1;
s301: as shown in fig. 2, the feeder is placed on the display board 2 and numbered, a ten-thousandth balance is used for weighing the empty feeder, 200 mu L of test liquid medicine is moved into the feeder by using the liquid feeder, an outer ring 51 of the liquid blocking ring 5 is fixed on the outer wall of the accommodating column 4 in a pasting mode, the inner ring 50 covers an opening of the accommodating groove 40 (shown in fig. 3), the total weight of the feeder (the total weight of the feeder comprises the total weight of the feeder, the liquid blocking ring 5 and the total weight of the test liquid medicine) is weighed again, the feeder and the liquid blocking ring 5 are quickly placed into the feeding box 1 together by using tweezers (shown in fig. 4), the lower part of the accommodating column 4 is connected with the bottom plate of the feeding box 1 through threads, the liquid blocking ring 5 is broken from the annular scratch 510 by pulling the top of the liquid blocking ring 5 by using the tweezers, the broken liquid blocking ring 5 is taken out from the annular scratch 510 (shown in fig. 5), the bee is allowed to freely take out the test liquid medicine of the accommodating groove 40 after 4h, the feeder is weighed, the broken liquid blocking ring 5 is taken out and the broken liquid 5 is connected with the broken liquid blocking ring which is taken out in the step in the weighing process (the tested liquid medicine is mixed with the tested liquid medicine of the same component of the tested liquid medicine of the bee through the test liquid in the test experiment liquid in the step of the prior art);
s302: after the feeder filled with the test liquid medicine is taken out, the feeder filled with the sucrose solution is placed in the feeder box 1 through tweezers, the groove opening of the containing groove 40 of the feeder is covered by the liquid blocking ring 5 after the sucrose solution is placed in the feeder, and after the feeder is fixed in the feeder box 1, the liquid blocking ring 5 is broken from the annular scratch 510 through pulling and the broken liquid blocking ring 5 is taken out; the subsequent experimental process is the same as the existing acute oral toxicity experimental process of bees, and vital signs of bees are observed and recorded;
bee acute contact toxicity test was performed:
s310, covering a cover on the centrifuge tube 3 in S2, wherein a pneumatic quick connector (shown in figure 1) is arranged on the cover of the centrifuge tube 3, one end of the pneumatic quick connector is connected with a carbon dioxide gas bottle through a hose, the other end of the pneumatic quick connector stretches into an opening of the cover of the centrifuge tube 3, a steel bottle main valve is unscrewed, a pressure valve is regulated to 0.02Mpa, after 4S of anesthesia, bees are placed on a test bench, the wings of the bees are clamped by forceps, 1 mu L of test liquid medicine is dripped to a middle chest backboard of the bees by a micro-drip instrument or a pipette, and the bees are transferred into a feeding box 1 after the solvent volatilizes;
s311, placing a feeder filled with sucrose solution in the feeding box 1; as in the above steps, the slot opening of the containing groove 40 of the feeder is covered by the liquid retaining ring 5 after the feeder is always placed with the sucrose solution, and then the liquid retaining ring 5 is broken by pulling from the annular scratch 510 and the broken liquid retaining ring 5 is taken out after the feeder is fixed in the feeding cassette 1.
As shown in fig. 2-5, the feeder in the steps of the method comprises a containing column 4, the top end of the containing column 4 is provided with a containing groove 40, the opening of the containing groove 40 is arranged on the top of the containing column 4 at the side part of the containing groove 40 relative to the containing groove 40, the containing groove 40 is cylindrical, the aperture of the containing groove 40 is 7mm, the volume of the containing groove 40 is slightly more than 200 mu L, the opening of the containing groove 40 only allows the body of one bee to extend into the liquid in the containing groove 40, so that two or more bees can be prevented from eating the liquid in the containing groove 40 at the same time, and the feeder is prevented from toppling;
in order to further avoid tilting of the feeder, in this embodiment, preferably, the lower end of the accommodating column 4 is provided with external threads, the bottom plate of the feeding box 1 is provided with a threaded hole, and the threaded hole is matched with the external threads at the lower end of the accommodating column 4;
the feeding box further comprises a display board 2, wherein the display board 2 is provided with a containing hole 20, the containing column 4 is vertically and slidably arranged in the containing hole 20, the containing column 4 is prevented from toppling over when the feeding box 1 is arranged outside, and when the feeder is not arranged in the feeding box, the feeder is arranged in the containing hole 20 of the display board 2;
the feeder also comprises a liquid blocking ring 5, the liquid blocking ring 5 is arranged on the accommodating column 4 and is used for preventing solution from splashing out of the accommodating groove 40, the liquid blocking ring 5 is wrapped on the opening of the accommodating groove 40, the liquid blocking ring 5 is conical, the inner diameter of the liquid blocking ring 5 gradually decreases from one end close to the accommodating groove 40 to one end far away from the accommodating groove 40, the diameter of one end of the liquid blocking ring 5 far away from the accommodating groove 40 is 1mm-4mm, namely the aperture of the opening of the liquid blocking ring 5 is 1mm-4mm, so that liquid in the accommodating groove 40 is prevented from leaking out of the feeder in the process of placing the feeder into the feeding box 1, the conical liquid blocking ring 5 is convenient for discharging the liquid on the inner wall of the liquid blocking ring 5 after placing the feeder into the accommodating groove 40, the diameter of one end of the liquid blocking ring 5 far away from the accommodating groove 40 is 1mm-4mm, and the liquid is prevented from being discharged from the opening of the liquid blocking ring 5 under the action of surface tension of the liquid itself;
the liquid blocking ring 5 is made of thinner paper, the outer side wall of the liquid blocking ring 5 is provided with annular scratches 510 along the circumferential direction of the liquid blocking ring, when the liquid blocking ring 5 and the feeder are placed in the feeding box 1 together, the liquid blocking ring 5 is broken from the annular scratches 510 by pulling the liquid blocking ring 5, and the part of the liquid blocking ring 5 separated from the feeder is taken out from the feeding box 1;
the liquid blocking ring 5 comprises an inner ring 50 and an outer ring 51, the inner ring 50 is communicated with the accommodating groove 40, the lower end of the inner ring 50 is abutted against the upper end face of the accommodating column, the inner ring 50 is conical, the outer ring is cylindrical, the outer ring 51 is sleeved and fixed on the outer wall of the lower end of the inner ring 50, the outer ring 51 is fixed on the outer wall of the accommodating column 4, the annular scratch 510 is formed on the outer ring 51, the annular scratch 510 and the upper end face of the accommodating column 4 are arranged in the same plane, the liquid blocking ring 5 is convenient to connect with a feeder, and meanwhile, the liquid blocking ring 5 is convenient to separate along the annular scratch 510 so as to seal dietary liquid from the opening of the accommodating groove 40;
when placing the feeder, will hold post 4 through tweezers and place the screw hole department of feeding box 1 then rotate and hold post 4, make the axis rotation that holds post 4 along vertical direction make the lower extreme that holds post 4 and place the bottom plate of box and pass through the screw thread mode and be connected, in rotating the in-process also be convenient for keep off liquid flow of liquid ring 5 inner wall to holding tank 40, then tear up and keep off liquid ring 5 and take out the fender liquid ring 5 of tearing up from feeding box 1, will hold post 4 and take out and place in the accommodation hole 20 of panel 2 through tweezers after a certain time.
The invention can collect bees rapidly and avoid hurting bees. The bee acute oral toxicity test can be carried out without anaesthetizing bees, and the invention of the feeder can solve the problem of incomplete feeding of liquid medicine and avoid the risk of liquid medicine dripping. The bee acute contact toxicity test can be repeated to independently anesthetize the bees, so that the whole anesthesia is avoided, and the damage to the bees is reduced; the whole set of novel method makes the test more scientific and reasonable.
The foregoing is only a preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art, who is within the scope of the present invention, should make equivalent substitutions or modifications according to the technical scheme of the present invention and the inventive concept thereof, and should be covered by the scope of the present invention.
Claims (6)
1. A bee acute oral toxicity test and an acute contact toxicity test method, which are characterized by comprising the following steps:
starvation treatment:
placing a certain number of bees into a rearing cage, and starving for 1-3 hours under the dark condition;
acute oral toxicity test of bees:
placing the starved bees into a feeding box;
placing the experimental liquid medicine into a feeder and weighing the feeder filled with the experimental liquid medicine, placing the weighed feeder into a feeding box, taking out the feeder after 3-6 hours, and weighing the feeder;
after bees in the feeder absorb a certain amount of experimental liquid medicine, the feeder filled with sucrose solution is placed in the feeding box;
bee acute contact toxicity test was performed:
placing the starved bees into a centrifuge tube, introducing carbon dioxide into the centrifuge tube for anesthesia for 3-5 s, placing the bees on a test bench, clamping wings of the bees by using forceps, dropping 1 mu L of test liquid medicine to a middle chest backboard of the bees by using a micro-dropper or a pipette, and transferring the bees into a feeding box after the solvent volatilizes;
placing a feeder containing sucrose solution in a feeder box;
the feeder comprises a containing column, and a containing groove is formed in the top end of the containing column;
the liquid blocking ring is arranged on the accommodating column and used for preventing the solution from splashing out of the accommodating groove;
the outer side wall of the liquid blocking ring is provided with annular scratches along the circumferential direction of the liquid blocking ring;
the liquid blocking ring comprises an inner ring and an outer ring, the inner ring is communicated with the accommodating groove, the outer ring is sleeved and fixed on the outer wall of the inner ring, the outer ring is fixed on the outer wall of the accommodating column, the annular scratch is formed on the outer ring, and the annular scratch and the upper end face of the accommodating column are in the same plane.
2. The method for acute oral toxicity test and acute contact toxicity test of bees according to claim 1, wherein the pore diameter of the holding groove is 7mm.
3. The method for testing bee acute oral toxicity and acute contact toxicity according to claim 1, further comprising a display board, wherein the display board is provided with a containing hole, and the containing column is vertically and slidably arranged in the containing hole.
4. The method for testing bee acute oral toxicity and acute contact toxicity according to claim 1, wherein the liquid blocking ring is tapered, and the inner diameter of the liquid blocking ring is gradually reduced from one end close to the accommodating groove to one end far from the accommodating groove.
5. The bee acute oral toxicity test and acute contact toxicity test method according to any one of claims 1 to 4, wherein the lower end of the receiving column is connected to the bottom plate of the feeding box by screw thread.
6. The bee acute oral toxicity test and acute contact toxicity test method according to claim 1, wherein a pneumatic quick connector is fixed on the cover of the centrifuge tube, and carbon dioxide is introduced into the centrifuge tube through a hose connection pneumatic quick connector.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210716618.2A CN115152702B (en) | 2022-06-23 | 2022-06-23 | Bee acute oral toxicity test and acute contact toxicity test method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210716618.2A CN115152702B (en) | 2022-06-23 | 2022-06-23 | Bee acute oral toxicity test and acute contact toxicity test method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115152702A CN115152702A (en) | 2022-10-11 |
| CN115152702B true CN115152702B (en) | 2023-11-03 |
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| CN202210716618.2A Active CN115152702B (en) | 2022-06-23 | 2022-06-23 | Bee acute oral toxicity test and acute contact toxicity test method |
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| JPH0423653U (en) * | 1990-06-21 | 1992-02-26 | ||
| JPH06329160A (en) * | 1993-05-17 | 1994-11-29 | Keisuke Ito | Complex container retaining freshness after unseal and its production |
| JPH119657A (en) * | 1997-06-24 | 1999-01-19 | Eisai Co Ltd | Mouth piece for medicine bottle |
| KR19990012774U (en) * | 1997-09-12 | 1999-04-15 | 이태환 | Snap cap of the container |
| RU50374U1 (en) * | 2005-08-01 | 2006-01-20 | Николай Александрович Помазуев | CEILING BEER FEEDER |
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