CN108566903A - Honeybee acute oral toxicity test device - Google Patents
Honeybee acute oral toxicity test device Download PDFInfo
- Publication number
- CN108566903A CN108566903A CN201710145962.XA CN201710145962A CN108566903A CN 108566903 A CN108566903 A CN 108566903A CN 201710145962 A CN201710145962 A CN 201710145962A CN 108566903 A CN108566903 A CN 108566903A
- Authority
- CN
- China
- Prior art keywords
- honeybee
- toxicity test
- oral toxicity
- acute oral
- bee cage
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 241000256844 Apis mellifera Species 0.000 title claims abstract description 165
- 238000012360 testing method Methods 0.000 title claims abstract description 47
- 231100000460 acute oral toxicity Toxicity 0.000 title claims abstract description 41
- 239000007788 liquid Substances 0.000 claims abstract description 49
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 16
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 8
- 239000004677 Nylon Substances 0.000 claims description 22
- 229920001778 nylon Polymers 0.000 claims description 22
- 239000006188 syrup Substances 0.000 claims description 13
- 235000020357 syrup Nutrition 0.000 claims description 13
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 10
- 229910000831 Steel Inorganic materials 0.000 claims description 5
- 229910002092 carbon dioxide Inorganic materials 0.000 claims description 5
- 239000001569 carbon dioxide Substances 0.000 claims description 5
- 229910001220 stainless steel Inorganic materials 0.000 claims description 5
- 239000010935 stainless steel Substances 0.000 claims description 5
- 239000010959 steel Substances 0.000 claims description 5
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims 1
- 239000000126 substance Substances 0.000 abstract description 8
- 230000000694 effects Effects 0.000 abstract description 7
- 239000000575 pesticide Substances 0.000 abstract description 7
- 238000004140 cleaning Methods 0.000 abstract description 5
- 230000001154 acute effect Effects 0.000 abstract description 4
- 238000009423 ventilation Methods 0.000 abstract description 4
- 231100000331 toxic Toxicity 0.000 abstract description 2
- 230000002588 toxic effect Effects 0.000 abstract description 2
- 239000003814 drug Substances 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 238000012545 processing Methods 0.000 description 5
- 239000000203 mixture Substances 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
- 206010058667 Oral toxicity Diseases 0.000 description 2
- 208000010513 Stupor Diseases 0.000 description 2
- 231100000403 acute toxicity Toxicity 0.000 description 2
- 230000007059 acute toxicity Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 231100000418 oral toxicity Toxicity 0.000 description 2
- 238000012856 packing Methods 0.000 description 2
- 230000000607 poisoning effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- 231100000820 toxicity test Toxicity 0.000 description 2
- 206010010071 Coma Diseases 0.000 description 1
- 206010015548 Euthanasia Diseases 0.000 description 1
- 208000005374 Poisoning Diseases 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000013401 experimental design Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 231100000636 lethal dose Toxicity 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K47/00—Beehives
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K53/00—Feeding or drinking appliances for bees
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a kind of honeybee acute oral toxicity test device, the experimental rig has an at least honeybee collector, at least a liquid feeder and at least bee cage mutually dispensed；The honeybee collector is the honeybee collector for acquiring honeybee and can be connected to nitrogen cylinder；The liquid feeder is the liquid feeder of the honeybee progress oral administration for being acquired to the honeybee collector；The bee cage is the bee cage that acute oral toxicity test is carried out to the honeybee after the liquid feeder oral administration, and the liquid feeder carries out oral administration in the bee cage to the honeybee of honeybee collector acquisition.The present invention provide it is a kind of convenient for cleaning, durable, safe and reliable, ventilation effect is good, while can precisely quantitatively dispenser, and then the honeybee acute oral toxicity test device of preferably acute toxic effects such as evaluating chemical product or pesticide.
Description
Technical field
The present invention relates to honeybee acute oral toxicity test technical fields more particularly to a kind of honeybee acute oral toxicity to try
Experiment device.
Background technology
After acute toxicity refers to primary (or in 24 hours repeatedly) the contact foreign compound of body (people or experimental animal)
Caused poisoning effect, or even cause death.Acute oral toxicity test refers to the examination contaminated once or repeatedly in 24 hours
It tests, is the premise of the toxicity research such as chemicals or pesticide.Wherein, honeybee acute oral toxicity test is using honeybee as acute
The subjects of Oral toxicity experiment, are mainly used for measuring the toxicity to chemicals or pesticide etc., are the poison such as chemicals or pesticide
Journal of Sex Research provides chemical basis.
For honeybee acute oral toxicity test device in the prior art, such as a kind of honeybee of patent CN203968939U
Acute oral toxicity test device etc. is integrally formed toxicity test device.But integrally formed urgency in the prior art
Property Oral toxicity experimental rig exist be not easy to cleaning treatment, ventilation effect is undesirable during the test, to be unfavorable for protecting
The problems such as confirming the survival of the honeybee during testing.
Invention content
The present invention exists for honeybee acute oral toxicity test device in the prior art is not easy to cleaning treatment, divulges information
Effect is undesirable, and it is an object of the present invention to provide a kind of convenient for clear the defects of to be unfavorable for ensureing the survival of the honeybee in experimentation
Wash, be durable, is safe and reliable, ventilation effect it is good, while can precisely quantitatively dispenser, and then preferably evaluating chemical product or
The honeybee acute oral toxicity test device of the acute toxic effects such as pesticide.
Realizing the technical solution of above-mentioned purpose is：
The honeybee acute oral toxicity test device of the present invention, the experimental rig have at least honeybee mutually dispensed
Collector, at least a liquid feeder and at least a bee cage；
" packing " refers to that the honeybee acute oral toxicity test device of the present invention is fed by honeybee collector, liquid
Device, bee cage, honeybee put to death bottle and honeybee syrup feeder is integrally formed composition, but by respectively independent honeybee acquires mutually
Device, liquid feeder, bee cage, honeybee put to death bottle and honeybee syrup feeder composition.
The honeybee collector is the honeybee collector for acquiring honeybee and can be connected to nitrogen cylinder；
The liquid feeder is the liquid feeding of the honeybee progress oral administration for being acquired to the honeybee collector
Device；
The bee cage is the bee cage that acute oral toxicity test is carried out to the honeybee after the liquid feeder dispenser.
The experimental rig, which also dispenses, has at least one processing that is used for that can be connected to carbon dioxide steel cylinder to carry out acute oral
The honeybee of honeybee after toxicity test puts to death bottle.
The experimental rig, which also dispenses, to be had at least one for carrying out syrup to dispenser treated honeybee in the bee cage
The honeybee syrup feeder of feeding ensures that honeybee survives during the test.
The bee cage tool has a square main body frame and one to be fixedly connected via several nylon ropes with the main body frame
For carrying out the nylon wire of acute oral toxicity test.
The nylon wire has one the nylon wire is closed to the opening tightened via nylon rope, and the honeybee collector is adopted
The honeybee of collection is transferred to via the opening of the nylon wire in the bee cage, liquid feeder the opening via the nylon wire
Mouth is placed into the bee cage or is taken out from the bee cage.
The liquid feeder is the medicine for being placed into the bee cage via stainless steel clip or being taken out from the bee cage
Liquid feeder ensures the safe and reliable of operation.
The liquid feeder is the medicine for being placed into the bee cage via stainless steel clip or being taken out from the bee cage
Liquid feeder ensures the safe and reliable of operation.
The positive effect of the present invention is that：
The honeybee acute oral toxicity test device of the present invention acquires honeybee by honeybee collector, is fed by liquid
Device carries out precisely quantitatively dispenser to the honeybee of acquisition, meanwhile, have convenient for cleaning, durable, ventilation effect is good, safety can
By the advantages that, compared with the existing technology in experimental rig, can preferably the acute toxicities such as evaluating chemical product or pesticide imitate
Fruit..
The technique effect of the design of the present invention, concrete structure and generation is described further below with reference to attached drawing, with
It is fully understood from the purpose of the present invention, feature and effect.
Description of the drawings
Fig. 1 is the structural schematic diagram of honeybee acute oral toxicity test device of the present invention；
Fig. 2 is the bee basket structure schematic diagram of honeybee acute oral toxicity test device of the present invention.
Specific implementation mode
With reference to Fig. 1 and Fig. 2, the honeybee acute oral toxicity test device of the present invention is described in detail.
As shown in Figure 1, the honeybee acute oral toxicity test device of the present embodiment, has mutually dispense at least one to be used for
Acquisition honeybee and can be connected to nitrogen cylinder honeybee collector 10, at least one honeybee for being used to acquire honeybee collector 10 into
The liquid feeder 20 of row oral administration, at least one to the honeybee after 20 dispenser of liquid feeder for carrying out acute oral toxicity
What the bee cage 30 of experiment, at least one can be connected to carbon dioxide steel cylinder is used to handle the honeybee after carrying out acute oral toxicity test
Honeybee put to death bottle 40 and at least one for during acute oral toxicity test to after dispenser sealing keep syrup feed
Honeybee syrup feeder 50, ensure honeybee survive during the test.Honeybee collector 10 is connected to nitrogen cylinder for inciting somebody to action
The honeybee of acquisition carries out stupor processing, but not injures honeybee.Liquid in liquid feeder 20 includes chemicals or pesticide etc..
" packing " refers to that the honeybee acute oral toxicity test device of the present invention is not by honeybee collector 10, liquid feeder 20, bee
Cage 30, honeybee put to death bottle 40 and honeybee syrup feeder 50 is integrally formed composition, but by respectively independent honeybee acquires mutually
Device 10, liquid feeder 20, bee cage 30, honeybee put to death bottle 40 and honeybee syrup feeder 50 forms.
As shown in Fig. 2, bee cage 30 has square main body frame 31 and consolidates via several nylon ropes 31 with main body frame 31
Surely the nylon wire 32 for carrying out acute oral toxicity test connected.Nylon wire 32, which has, pricks nylon wire 32 via nylon rope
Tight opening 33, the honeybee that honeybee collector 10 acquires are transferred to via the opening 33 of nylon wire 32 in bee cage 30, liquid feeding
Device 20 is placed into bee cage 30 via the opening 33 of nylon wire 32 or is taken out from bee cage 30.Liquid feeder 20 is via stainless
The liquid feeder 20 that steel clamp is placed into bee cage 30 or is taken out from bee cage 30 ensures the safe and reliable of operation.Bee cage 30
Nylon wire 32 have many advantages, such as convenient for cleaning and it is durable.
The operation principle of honeybee acute oral toxicity test device of the present invention is：
Step1 acquires honeybee using the honeybee collector 10 being connected to nitrogen cylinder, is acquired to the honeybee for collecting honeybee
It is filled with suitable nitrogen (flow velocity for being filled with nitrogen is 400mL/min, is filled with the time about 30 seconds) in device 10, honeybee is made to be in temporary
Comatose state ensures the safe and effective progress of acute oral toxicity test；Meanwhile honeybee syrup feeder 50 is placed into bee cage
In 30；
Step2, treated that honeybee is shifted by the opening 33 of bee cage 30 for the stupor that will be placed in honeybee collector 10
Into bee cage 30；Meanwhile before not being placed into bee cage 30 to liquid feeder 20, before first weighing and recording liquid is not added
First weight of liquid feeder 20 is re-weighed and records the second weight of the liquid feeder 20 after liquid is added, then adopts
It is placed into bee cage 30 with stainless steel clamp liquid feeder 20, the honeybee being transferred in bee cage 30 is applied
Medicine, and tighten the opening 33 of bee cage 30 prevents honeybee from flying out bee cage 30；(after general dispenser 4h, liquid consumes substantially after dispenser
It is complete；If honeybee refusal feed, spraying time at most extends to 6h), the opening 33 of bee cage 30 is opened, with stainless steel clip by medicine
Liquid feeder 20 takes out from bee cage 30, while being weighed to the liquid feeder 20 after dispenser and recording its third weight.
The difference that the first weight before liquid and the second weight being added after liquid is not added by liquid feeder 20 obtains addition medicine
The weight of liquid；It is added by liquid feeder 20 between the second weight after liquid and the third weight after being taken out in bee cage 30
Difference can be obtained the formulation rate of acute oral toxicity test process, pass through the processing of multiple gradients and the death rate of experimental design
Deng, you can calculate the half lethal dose to honeybee for examination object liquid.During acute oral toxicity test, using honeybee syrup
Feeder 50 keeps carrying out syrup supply to the honeybee after dispenser in bee cage 30, is observed and recorded according to test requirements document, such as observation note
Record processing experiment honeybee is in the poisoning symptom and The dead quantity for 24 hours, after 48h, in the case where the control group death rate is less than 10%,
If processing reaches 10% or more with the mortality difference after 48h for 24 hours, it is also necessary to observing time will extend to 96h；
Step3, after the test, the honeybee after progress acute oral toxicity test is centrally placed can be with carbon dioxide
The honeybee of steel cylinder connection is put to death in bottle 40, and being filled with enough carbon dioxide into honeybee execution bottle 40 carries out honeybee at euthanasia
Reason.
Each preferred embodiment of the present invention described in detail above.It should be appreciated that those skilled in the art
It according to the present invention can conceive without creative work and make many modifications and variations.Therefore, all skills in the art
Art personnel can be obtained by logical analysis, reasoning, or a limited experiment on the basis of existing technology under this invention's idea
Technical solution, all should be in the protection domain being defined in the patent claims.
Claims (6)
1. a kind of honeybee acute oral toxicity test device, which is characterized in that the experimental rig, which has, mutually to be dispensed at least
One honeybee collector, at least a liquid feeder and at least a bee cage；
The honeybee collector is the honeybee collector for acquiring honeybee and can be connected to nitrogen cylinder；
The liquid feeder is the liquid feeder of the honeybee progress oral administration for being acquired to the honeybee collector；
The bee cage is the bee cage that acute oral toxicity test is carried out to the honeybee after the liquid feeder dispenser.
2. honeybee acute oral toxicity test device as described in claim 1, which is characterized in that the experimental rig also dispenses
There is at least one honeybee for handling the honeybee after carrying out acute oral toxicity test that can be connected to carbon dioxide steel cylinder to put to death
Bottle.
3. honeybee acute oral toxicity test device as described in claim 1, which is characterized in that the experimental rig also dispenses
There is at least one honeybee syrup feeder for carrying out syrup feeding to dispenser treated honeybee in the bee cage, ensures honey
Bee survives during the test.
4. honeybee acute oral toxicity test device as described in claim 1, which is characterized in that the bee cage tool has a pros
Phosphor bodies frame and one is used to carry out acute oral toxicity test with the main body frame via what several nylon ropes were fixedly connected
Nylon wire.
5. honeybee acute oral toxicity test device as claimed in claim 4, which is characterized in that the nylon wire have once
The nylon wire is closed into the opening tightened, honeybee the opening via the nylon wire of the honeybee collector acquisition by nylon rope
It mouthful is transferred in the bee cage, the liquid feeder is placed into the bee cage via the opening of the nylon wire or from described
It is taken out in bee cage.
6. honeybee acute oral toxicity test device as described in claim 1, which is characterized in that the liquid feeder is warp
The liquid feeder for being placed into the bee cage by stainless steel clip or being taken out from the bee cage ensures that the safety of operation can
It leans on.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710145962.XA CN108566903A (en) | 2017-03-13 | 2017-03-13 | Honeybee acute oral toxicity test device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710145962.XA CN108566903A (en) | 2017-03-13 | 2017-03-13 | Honeybee acute oral toxicity test device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108566903A true CN108566903A (en) | 2018-09-25 |
Family
ID=63578436
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710145962.XA Withdrawn CN108566903A (en) | 2017-03-13 | 2017-03-13 | Honeybee acute oral toxicity test device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108566903A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115152702A (en) * | 2022-06-23 | 2022-10-11 | 安徽华辰检测技术研究院有限公司 | Bee acute oral toxicity test and acute contact toxicity test method |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2295238C1 (en) * | 2005-06-20 | 2007-03-20 | ГНУ Всероссийский научно-исследовательский институт ветеринарной энтомологии и арахнологии СО РАСХН | Apparatus for individual feeding of sugar syrup to bees on evaluation of toxicity of preparations and experimental infection with disease excitants |
| CN102640734A (en) * | 2012-05-17 | 2012-08-22 | 环境保护部南京环境科学研究所 | Method for carrying out bee colony anaesthesia by utilizing bee honeycomb anaesthesia device |
| CN104082186A (en) * | 2014-07-28 | 2014-10-08 | 环境保护部南京环境科学研究所 | Bee acute oral toxicity experimental apparatus |
| CN204272919U (en) * | 2014-09-24 | 2015-04-22 | 苏州西山中科药物研究开发有限公司 | A kind of honeybee acute toxicity experience testing arrangement |
| CN204945857U (en) * | 2015-09-25 | 2016-01-06 | 北京依科世福科技有限公司 | A kind of honeybee toxicity test device |
| CN105850898A (en) * | 2016-05-31 | 2016-08-17 | 中国农业科学院蜜蜂研究所 | Method for measuring acute and chronic toxicity of drug against varroa jacobsoni |
-
2017
- 2017-03-13 CN CN201710145962.XA patent/CN108566903A/en not_active Withdrawn
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2295238C1 (en) * | 2005-06-20 | 2007-03-20 | ГНУ Всероссийский научно-исследовательский институт ветеринарной энтомологии и арахнологии СО РАСХН | Apparatus for individual feeding of sugar syrup to bees on evaluation of toxicity of preparations and experimental infection with disease excitants |
| CN102640734A (en) * | 2012-05-17 | 2012-08-22 | 环境保护部南京环境科学研究所 | Method for carrying out bee colony anaesthesia by utilizing bee honeycomb anaesthesia device |
| CN104082186A (en) * | 2014-07-28 | 2014-10-08 | 环境保护部南京环境科学研究所 | Bee acute oral toxicity experimental apparatus |
| CN204272919U (en) * | 2014-09-24 | 2015-04-22 | 苏州西山中科药物研究开发有限公司 | A kind of honeybee acute toxicity experience testing arrangement |
| CN204945857U (en) * | 2015-09-25 | 2016-01-06 | 北京依科世福科技有限公司 | A kind of honeybee toxicity test device |
| CN105850898A (en) * | 2016-05-31 | 2016-08-17 | 中国农业科学院蜜蜂研究所 | Method for measuring acute and chronic toxicity of drug against varroa jacobsoni |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115152702A (en) * | 2022-06-23 | 2022-10-11 | 安徽华辰检测技术研究院有限公司 | Bee acute oral toxicity test and acute contact toxicity test method |
| CN115152702B (en) * | 2022-06-23 | 2023-11-03 | 安徽华辰检测技术研究院有限公司 | Bee acute oral toxicity test and acute contact toxicity test method |
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|---|---|---|---|
| PB01 | Publication | ||
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| SE01 | Entry into force of request for substantive examination | ||
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| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20180925 |
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| WW01 | Invention patent application withdrawn after publication |