CN101856010A - Operating device for anaesthetizing active insects - Google Patents
Operating device for anaesthetizing active insects Download PDFInfo
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- CN101856010A CN101856010A CN201010196623A CN201010196623A CN101856010A CN 101856010 A CN101856010 A CN 101856010A CN 201010196623 A CN201010196623 A CN 201010196623A CN 201010196623 A CN201010196623 A CN 201010196623A CN 101856010 A CN101856010 A CN 101856010A
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Abstract
The invention provides an operating device for anaesthetizing active insects. The device comprises a carbon dioxide cylinder (6), an operating table (2) and a pressure gun (4), wherein the operating table (2) and the pressure gun (4) are connected with the carbon dioxide cylinder (6) respectively. The operating device for anaesthetizing the active insects of the invention utilizes the principle that stigmas of a majority of insects are temporarily closed to in an anaesthetized and unconscious state in a high-concentration carbon oxide environment for the convenient observation or technical operation of the active insects; and the anaesthetized insects may revive naturally when moved to an environment without carbon oxide. The operating device has the advantages of avoiding the damage to the interior of an insect body and the adverse impact on a human body possibly caused by using the conventional ether for anaesthetizing the active insects and reducing indoor noxious solvent contamination.
Description
Technical field
The invention belongs to the agricultural insect technical field, particularly relate to the operating means that a kind of anesthesia enlivens insect.
Background technology
In the agricultural insect research process, the scientific research personnel carries out close-ups or work study through regular meeting to some insects indoor, but carries out aforesaid operations and often need certain pre-treatment process for enlivening insect.
The following two kinds of methods of the general employing of prior art:
Therefore, people press for the operating means that a kind of anesthesia enlivens insect.On the one hand, easy to operation, little to targeted insect and operating personnel's negative effect on the other hand, reduce environmental pollution.
Summary of the invention
The purpose of this invention is to provide a kind of operating means that enlivens insect that is used to anaesthetize, to solve the defective that prior art exists.
The invention provides the operating means that a kind of anesthesia enlivens insect, this device comprises: dioxide bottle, operating desk and pressure gun; Wherein, operating desk links to each other with dioxide bottle respectively with pressure gun.
Described operating desk links to each other with dioxide bottle respectively by wireway with pressure gun.
Described wireway one end links to each other with dioxide bottle, and the other end is divided into two arms, links to each other with pressure gun with operating desk respectively; Wherein, operating personnel can carry out test operation on described operating desk; Described pressure gun can be controlled carbon dioxide and discharge.
Have switch on the arm of the wireway that links to each other with operating desk.
Dioxide bottle is with being with manometric gas pressure reducer, and it exports pressure≤0.1Mpa, can regulate and control the flow of carbonic acid gas.Preferably, cylinder liner is filled with high-purity (99.9999%) carbon dioxide.
Described operating desk is tip the plastic casing that covers, but inner chamber filling CO 2 gas is arranged wherein, cartridge upper surface has diameter to be 0.2 centimetre～0.5 centimetre to become the circular hole of 12 * 8 spread patterns and cover 80 orders～200 purpose nylon screens, can guarantee that carbonic acid gas discharges from sieve screen apertures.
Described operating desk inner chamber below has mouth can connect wireway.
Described pressure gun is with gas switch, 0.2 centimetre～0.4 centimetre of gun barrel diameter; Controllable being input in vial or the foster scolite of finger-type glass of the carbon dioxide of wireway (can be used for tested insect is put and anaesthetized in advance).Preferably, described vial diameter 6cm～9cm, high 11cm～15cm or finger-type glass are supported scolite diameter 2.5cm～5cm, high 8cm～10cm.Vial or finger-type glass are supported scolite can prevent to enliven the insect escape with tampon or breathable TR thin rubber cap seal mouth.
Stereomicroscope can be observed by the anesthesia insect line operate of going forward side by side, and it be the continuous type microscope, eyepiece 10X, and object lens 0.7X-4.5X, total magnification: 7X～45X is furnished with up and down and throws light on.
Operating means of the present invention also comprises a vial or a foster scolite of finger-type glass of containing tested insect, is used for pressure gun is inserted in vial or the foster scolite of finger-type glass, and starting pressure switch release of carbon dioxide is anaesthetized tested insect.
The present invention also provides the aforesaid operations device being used for anaesthetizing the application that enlivens insect, in the laboratory.
Utilize operating means of the present invention, pressure gun is inserted in vial or the foster scolite of finger-type glass, starting pressure switch release of carbon dioxide makes to be enlivened the temporary close of insect belly valve and is seemingly-dead phenomenon by anesthesia, subsequently dopey insect is moved on on the operating desk of carbonic acid gas release, tested insect will be in by narcosis always, can observe or carry out other anatomy experiment research under stereomicroscope.
Wherein, described stereomicroscope is the continuous type microscope, eyepiece 10X, and object lens 0.7X-4.5X, total magnification: 7X～45X is furnished with illumination up and down.
The operating means that anesthesia of the present invention enlivens insect has following beneficial effect:
1) easy to operation.
2) to not injury of targeted insect, to operating personnel also almost not influence, reduce the organic solvent environmental pollution, be convenient to operation and help protecting environment simultaneously.
Description of drawings
Fig. 1 enlivens the operating means of insect for the present invention's anesthesia;
Among the figure: 1, stereomicroscope; 2, operating desk; 3, wireway; 4, pressure gun; 5, vial or finger-type glass are supported scolite; 6, dioxide bottle; 7, switch.
Embodiment
Following examples are used to illustrate the present invention, but are not used for limiting the scope of the invention.
As shown in Figure 1, the anesthesia of the present invention operating means that enlivens insect comprises: dioxide bottle 6, operating desk 2 and pressure gun 4; Wherein, operating desk 2 links to each other with dioxide bottle 6 respectively with pressure gun 4.
Described operating desk 2 links to each other with dioxide bottle 6 respectively by wireway 3 with pressure gun 4.
Described wireway 3 one ends link to each other with dioxide bottle 6, and the other end is divided into two arms, link to each other with pressure gun 4 with operating desk 2 respectively; Wherein, operating personnel can carry out test operation on described operating desk 2; Described pressure gun 4 can be controlled carbon dioxide and discharge.
Have switch 7 on the arm of the wireway 3 that links to each other with operating desk 2.
Dioxide bottle 6 is with being with manometric gas pressure reducer, and it exports pressure≤0.1Mpa, can regulate and control the flow of carbonic acid gas.Preferably, cylinder liner is filled with high-purity (99.9999%) carbon dioxide.
Described operating desk 2 is tip plastic casings that cover, but inner chamber filling CO 2 gas is arranged wherein, cartridge upper surface has diameter to be 0.2 centimetre～0.5 centimetre to become the circular hole of 12 * 8 spread patterns and cover 80 orders～200 purpose nylon screens, can guarantee that carbonic acid gas discharges from sieve screen apertures.
Described operating desk 2 inner chambers below has mouth can connect wireway.
Described pressure gun 4 is with gas switch, 0.2 centimetre～0.4 centimetre of gun barrel diameter; Controllable being input in vial or the foster scolite of finger-type glass of the carbon dioxide of wireway (can be used for tested insect is put and anaesthetized in advance).Preferably, described vial diameter 6cm～9cm, high 11cm～15cm or finger-type glass are supported scolite diameter 2.5cm～5cm, high 8cm～10cm.Vial or finger-type glass are supported scolite can prevent to enliven the insect escape with tampon or breathable TR thin rubber cap seal mouth.
Stereomicroscope can be observed by the anesthesia insect line operate of going forward side by side, and it be the continuous type microscope, eyepiece 10X, and object lens 0.7X-4.5X, total magnification: 7X～45X is furnished with up and down and throws light on.
It is as follows that the present invention anaesthetizes the operating means concrete operations that enliven insect:
Carbonic acid gas discharges along wireway 3 from gas cylinder 6 and is split into two parts, and a part of carbonic acid gas is transported in the pressure gun 4 with pressure switch.Another part carbonic acid gas is transported in the operating desk 2 by switch 7, and operating desk 2 can be placed under the stereomicroscope A.During operation, earlier active insect is placed vial or finger-type glass to support in the scolite 5 and with tampon or breathable TR thin rubber cap seal mouth.Open gas cylinder 6 pressure valve and switch 7 make pressure rob 4 and operating desk 2 be full of carbonic acid gas.Pressure gun 4 is inserted in vial or the foster scolite 5 of finger-type glass, starting pressure switch release of carbon dioxide makes to be enlivened the temporary close of insect belly valve and is seemingly-dead phenomenon by anesthesia, subsequently dopey insect is moved on on the operating desk 2 of carbonic acid gas release, tested insect will be in by narcosis always, can observe or carry out other anatomy experiment research under stereomicroscope A.
Observe 5 instar bollworm grubs in experimental example 1 laboratory
Control methods: earlier cotton bollworm larvae is placed in the glass tube, put into the cotton balls that was soaked by the 0.5ml ether, airtight.Cotton bollworm larvae is by smoked dizzy paralysis, after move on to stereomicroscope and observe for 1 time.Because the strong fumigating effect of ether also can be felt dizziness and nausea even operating personnel wear masks, indoor also have a very big ether taste.And the grasp of ether dosage is bad, and the low cotton bollworm of dosage can be waken up midway and be come, and revive after the words cotton bollworm test that dosage is big finishes and do not come over, and indoor pollution is heavy.
Adopt apparatus of the present invention, cotton bollworm larvae is temporarily benumbed by carbonic acid gas earlier, and it is moved to back on the operating desk owing to continue to have carbonic acid gas to surround, and presents the Somnolence always, is convenient to observe.Carbonic acid gas is a part of air simultaneously, and little to operating personnel's negative effect, environmental pollution is low.When cotton bollworm is moved under the no carbon dioxide environment, cotton bollworm revives very soon.
Observe in experimental example 2 laboratories and distinguish female male drosophila adult
Control methods: with fruit bat place reduce its liveness on ice after, move to operating desk and observe evaluation.Because the injury from low temperature result, after observation finished, fruit bat did not all revive.
Adopt apparatus of the present invention fruit bat temporarily to be benumbed by carbonic acid gas earlier, it is moved to back on the operating desk owing to continue to have carbonic acid gas to surround, present the Somnolence always, be convenient to observe.Carbonic acid gas is little to operating personnel's negative effect simultaneously, and environmental pollution is low.When the fruit bat adult is moved under the no carbon dioxide environment, fruit bat revives very soon, the flight of spreading the wings.
Claims (10)
1. an anesthesia enlivens the operating means of insect, it is characterized in that this device comprises: dioxide bottle (6), operating desk (2) and pressure gun (4); Wherein, operating desk (2) links to each other with dioxide bottle (6) respectively with pressure gun (4).
2. operating means according to claim 1 is characterized in that, described operating desk (2) links to each other with dioxide bottle (6) respectively by wireway (3) with pressure gun (4).
3. operating means according to claim 2 is characterized in that, described wireway (3) one ends link to each other with dioxide bottle (6), and the other end is divided into two arms, links to each other with pressure gun (4) with operating desk (2) respectively.
4. operating means according to claim 2 is characterized in that, has switch (7) on the arm of the wireway (3) that links to each other with operating desk (2).
5. operating means according to claim 1 is characterized in that, dioxide bottle (6) is with being with manometric gas pressure reducer, and it exports pressure≤0.1Mpa.
6. operating means according to claim 1, it is characterized in that, described operating desk (2) is tip the plastic casing that covers, inner chamber filling CO 2 gas is wherein arranged, and cartridge upper surface has diameter to be 0.2 centimetre～0.5 centimetre to become the circular hole of 12 * 8 spread patterns and cover 80 orders～200 purpose nylon screens.
7. operating means according to claim 6 is characterized in that, described operating desk (2) inner chamber below has mouth and wireway to join.
8. operating means according to claim 1 is characterized in that, described pressure gun (4) is with gas switch, 0.2 centimetre～0.4 centimetre of gun barrel diameter.
9. operating means according to claim 1, it is characterized in that, also comprise a vial or a foster scolite (5) of finger-type glass of containing tested insect, be used for pressure gun is inserted in vial or the foster scolite (5) of finger-type glass that starting pressure switch (4) release of carbon dioxide is anaesthetized tested insect.
10. each described operating means of claim 1-9 is being used for anaesthetizing the application that enlivens insect.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101966232A CN101856010B (en) | 2010-06-02 | 2010-06-02 | Operating device for anaesthetizing active insects |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101966232A CN101856010B (en) | 2010-06-02 | 2010-06-02 | Operating device for anaesthetizing active insects |
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| Publication Number | Publication Date |
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| CN101856010A true CN101856010A (en) | 2010-10-13 |
| CN101856010B CN101856010B (en) | 2012-06-27 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2010101966232A Expired - Fee Related CN101856010B (en) | 2010-06-02 | 2010-06-02 | Operating device for anaesthetizing active insects |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101953720A (en) * | 2010-11-01 | 2011-01-26 | 同济大学 | Small-sized insect anesthesia apparatus |
| CN101999336A (en) * | 2010-11-01 | 2011-04-06 | 同济大学 | Small-size insect anesthesia apparatus |
| CN102640734A (en) * | 2012-05-17 | 2012-08-22 | 环境保护部南京环境科学研究所 | Method for carrying out bee colony anaesthesia by utilizing bee honeycomb anaesthesia device |
| CN102652504A (en) * | 2012-05-08 | 2012-09-05 | 吉林农业大学 | Novel method for collecting rice moth imago |
| CN112753676A (en) * | 2019-11-04 | 2021-05-07 | 何凤侠 | By CO2Adult device for anaesthetizing and selecting mosquitoes and flies |
-
2010
- 2010-06-02 CN CN2010101966232A patent/CN101856010B/en not_active Expired - Fee Related
Non-Patent Citations (5)
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| 《昆虫学报》 19951231 翦福记; 陈启宗; 陆安邦; 磷化氢、二氧化碳混合气体对腐食酪螨成螨的生物学效应 全文 1-10 , 第1期 2 * |
| 《昆虫知识》 19841231 柴春煊 介绍一种麻醉家蝇的新方法--乙醚低温麻醉法 全文 1-10 , 第6期 2 * |
| 《昆虫知识》 20011231 吴太平 CO_2麻醉蚊蝇配套用具 全文 1-10 , 第2期 2 * |
| 《贵州农业科学》 20100315 袁瑞，杨洪，张帆，申智慧 多代施用高效氯氰菊酯对异色瓢虫生物学特性的影响 第99-102页 5 第38卷, 第3期 2 * |
| 《首届全国生物化学与分子生物学教学研讨会论文集》 20071231 刘志学，桂馨，杨扬 简易果蝇麻醉装置和微麻醉操作台 第59-60页 1-10 , 2 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101953720A (en) * | 2010-11-01 | 2011-01-26 | 同济大学 | Small-sized insect anesthesia apparatus |
| CN101999336A (en) * | 2010-11-01 | 2011-04-06 | 同济大学 | Small-size insect anesthesia apparatus |
| CN101953720B (en) * | 2010-11-01 | 2012-01-25 | 同济大学 | Small-sized insect anesthesia apparatus |
| CN102652504A (en) * | 2012-05-08 | 2012-09-05 | 吉林农业大学 | Novel method for collecting rice moth imago |
| CN102652504B (en) * | 2012-05-08 | 2016-02-03 | 吉林农业大学 | A kind of new method of collecting rice moth imago |
| CN102640734A (en) * | 2012-05-17 | 2012-08-22 | 环境保护部南京环境科学研究所 | Method for carrying out bee colony anaesthesia by utilizing bee honeycomb anaesthesia device |
| CN102640734B (en) * | 2012-05-17 | 2014-06-04 | 环境保护部南京环境科学研究所 | Method for carrying out bee colony anaesthesia by utilizing bee honeycomb anaesthesia device |
| CN112753676A (en) * | 2019-11-04 | 2021-05-07 | 何凤侠 | By CO2Adult device for anaesthetizing and selecting mosquitoes and flies |
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| Publication number | Publication date |
|---|---|
| CN101856010B (en) | 2012-06-27 |
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