CN106212391B - Collection method and device for industrially producing living phytoseiulus persimilis - Google Patents
Collection method and device for industrially producing living phytoseiulus persimilis Download PDFInfo
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- CN106212391B CN106212391B CN201610625494.1A CN201610625494A CN106212391B CN 106212391 B CN106212391 B CN 106212391B CN 201610625494 A CN201610625494 A CN 201610625494A CN 106212391 B CN106212391 B CN 106212391B
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- 238000000034 method Methods 0.000 title claims abstract description 35
- 241000179203 Phytoseiulus persimilis Species 0.000 title description 9
- 241000238876 Acari Species 0.000 claims abstract description 28
- 230000003444 anaesthetic effect Effects 0.000 claims abstract description 16
- 239000000428 dust Substances 0.000 claims abstract description 15
- 241000238631 Hexapoda Species 0.000 claims abstract description 11
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 88
- 235000011089 carbon dioxide Nutrition 0.000 claims description 54
- 238000007789 sealing Methods 0.000 claims description 51
- 235000011511 Diospyros Nutrition 0.000 claims description 18
- 241000723267 Diospyros Species 0.000 claims description 18
- 229910002092 carbon dioxide Inorganic materials 0.000 claims description 17
- 239000001569 carbon dioxide Substances 0.000 claims description 17
- 206010002091 Anaesthesia Diseases 0.000 claims description 9
- 241000196324 Embryophyta Species 0.000 claims description 9
- 230000037005 anaesthesia Effects 0.000 claims description 9
- 238000005286 illumination Methods 0.000 claims description 6
- 238000009434 installation Methods 0.000 claims description 6
- 230000009471 action Effects 0.000 claims description 5
- 238000009395 breeding Methods 0.000 claims description 5
- 230000001488 breeding effect Effects 0.000 claims description 5
- 238000000926 separation method Methods 0.000 claims description 5
- 230000005484 gravity Effects 0.000 claims description 4
- 241000406588 Amblyseius Species 0.000 claims description 3
- 230000008676 import Effects 0.000 claims description 3
- 239000007787 solid Substances 0.000 claims description 3
- 241000179202 Phytoseiulus Species 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 claims description 2
- 241001481699 Phytoseiidae Species 0.000 claims 1
- 239000003994 anesthetic gas Substances 0.000 abstract description 12
- 230000000052 comparative effect Effects 0.000 description 14
- 239000007789 gas Substances 0.000 description 10
- 230000004083 survival effect Effects 0.000 description 10
- 235000002568 Capsicum frutescens Nutrition 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 3
- 241000607479 Yersinia pestis Species 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- JCXJVPUVTGWSNB-UHFFFAOYSA-N nitrogen dioxide Inorganic materials O=[N]=O JCXJVPUVTGWSNB-UHFFFAOYSA-N 0.000 description 3
- 238000004806 packaging method and process Methods 0.000 description 3
- 238000005303 weighing Methods 0.000 description 3
- 208000027418 Wounds and injury Diseases 0.000 description 2
- 230000001680 brushing effect Effects 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 238000009792 diffusion process Methods 0.000 description 2
- 208000014674 injury Diseases 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 241001380650 Chilia Species 0.000 description 1
- 241000426499 Chilo Species 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 241001454295 Tetranychidae Species 0.000 description 1
- 238000010923 batch production Methods 0.000 description 1
- 238000007664 blowing Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 244000062645 predators Species 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Catching Or Destruction (AREA)
Abstract
The invention discloses a method for collecting phytoseiid mites for industrially producing living bodies, which comprises the steps of placing host plants bred with the phytoseiid mites into a collection box body, then introducing anesthetic gas into the collection box body to anesthetize the phytoseiid mites, sucking the anesthetized insects into a wire mesh bag through a small dust collector arranged at the bottom of the collection box body so as to be conveniently metered and subpackaged, and then introducing the metered and subpackaged insects into a wind tunnel type reviving box to blow in fresh air for reviving. The invention also discloses a collecting device for industrially producing the living phytoseiid mites, which comprises a transparent collecting box body, an anesthetic supporting component and an insect body separating component.
Description
Technical Field
The invention relates to the technical field of artificial breeding of insects, in particular to a collecting method and a collecting device for industrially producing living phytoseiid mites.
Background
The phytoseiid persicae is a typical obligate predator, is a leaf-mites addicted, is an excellent predatory mite species for preventing and controlling the leaf mites, is valued in the field of international biological control, is introduced successively to prevent and control pest mites on cotton, vegetables, fruit trees and flower crops, and achieves remarkable prevention and control effect; the method is characterized in that: strong adaptability, large predation amount, strong fecundity, agile action, strong diffusion capacity and fierce sexual feeling; in recent years, commercial production of phytoseiid persimmons has been achieved in europe and america.
In the existing process of industrially producing living phytoseiid mites, most of separation and collection modes of phytoseiid mites are active brushing or passive diffusion, and the separation and collection efficiency is low, the phytoseiid mites escape seriously, the death rate is high and the like, so that the method is extremely undesirable; the disadvantages restrict the application of phytoseiid mites and the popularization of the green prevention and control technology of spider mites.
Therefore, how to provide a method or a device for collecting physeiilus persimilis with higher collection efficiency, less escape and lower mortality is a technical problem to be solved urgently by those skilled in the art.
Disclosure of Invention
The invention aims to provide a collection method for industrially producing living phytoseiid mites, which has the characteristics of higher collection efficiency, less escape and lower mortality. The invention also aims to provide a collecting device for realizing the collecting method for industrially producing the living physeiid mites.
In order to solve the technical problems, the technical scheme provided by the invention is as follows:
the utility model provides a collection method of little phytoseiid mite of living body of batch production, will raise the host plant that has little phytoseiid mite of chilies and put into the collection box, then to leading-in anesthetic gas in the collection box makes little phytoseiid mite of chilies anesthesia, then through with the small-size dust catcher that collects the box intercommunication with little phytoseiid mite suction after will anaesthetizing to the silk screen bag in, then to little phytoseiid mite of chilies in the silk screen bag carry out measurement and partial shipment, then with the little phytoseiid mite of chilies after measurement and partial shipment put into the wind tunnel type and revive the incasement and insufflate fresh air and revive.
Preferably, the anesthetic gas is nitrogen or carbon dioxide.
The invention also provides a collecting device for realizing the collecting method for the living phytoseiulus persimilis industrially produced, which comprises a transparent collecting box body, an anesthetic supporting component and an insect body separating component;
the interior of the collecting box body is of a cavity structure, and a host plant for breeding phytoseiulus persimilis can be placed in the collecting box body;
the collecting box body is a sealed box body with only an opening on the front side surface, and a movable sealing door is arranged on the front side surface of the collecting box body and used for opening or closing the collecting box body;
the top wall of the collection box body is provided with at least two through holes for placing dry ice bowls into the collection box body;
the anesthetic support assembly comprises a dry ice bowl for storing solid anesthetic dry ice and a dry ice bowl support frame for supporting the dry ice bowl in the collection box body, the dry ice bowl support frame is fixedly arranged on the inner wall surface of the collection box body and is positioned at the upper part of the inner space of the collection box body, the dry ice bowl is placed on the dry ice bowl support frame so that low-temperature carbon dioxide gas is generated after the dry ice is gasified, and the low-temperature carbon dioxide gas flows from top to bottom in the collection box body so as to be used for anesthetizing the amblyseius persimmons persicae;
the worm body separating component comprises a wire mesh bag, a worm body suction hose and a small dust collector;
polypide suction hose set up in the bottom of collection box, just the upper end of polypide suction hose runs through the diapire of collection box stretches into in the collection box, polypide suction hose's lower extreme with small-size dust catcher's suction opening intercommunication, the silk screen bag set up in polypide suction hose's import department, in order to be used for the separation to collect little phytoseiulus persicae of chilia after the internal anesthesia of collection box.
Preferably, a sliding groove for embedding the movable sealing door is arranged on the edge of the opening of the collecting box body; the movable sealing door is embedded in the chute and can freely slide up and down along the chute so as to open or close the collecting box body; and a sealing gasket strip for sealing is arranged in the sliding groove.
Preferably, a handle is fixedly arranged at the top of the movable sealing door, and a holding space is arranged in the handle and used for an operator to pull the movable sealing door to slide up and down through the handle.
Preferably, the dry ice collecting box further comprises an air pressure balancing assembly, the air pressure balancing assembly comprises a vent valve, the vent valve is arranged on the movable sealing door and located at the top of the movable sealing door, and one end of the vent valve is communicated with the inner cavity structure of the collecting box body to balance air pressure inside and outside the collecting box body after dry ice is gasified.
Preferably, the anesthetic support assembly comprises a support spring, a guide cylinder, a support connecting plate, a bottom plate and a support sleeve;
the upper end of the supporting spring is fixedly connected to the inner wall of the top plate of the collecting box body and is positioned at the side part of the through hole, the supporting spring extends downwards, and the lower end of the supporting spring is fixedly connected to the supporting connecting plate;
the supporting connecting plate is arranged on the side part of the bottom plate and extends outwards;
the guide cylinder is arranged on the supporting connecting plate, and the lower end of the supporting spring extends downwards into the guide cylinder and is fixedly connected to the supporting connecting plate so as to be guided and limited by the guide cylinder;
the supporting sleeve is arranged on the bottom plate and extends upwards along the side edge of the bottom plate, so that an installation space is formed on the bottom plate;
an external dry ice bowl is placed in the installation space on the bottom plate through the through hole, the supporting spring stretches downwards under the action of the gravity of the dry ice bowl, so that the dry ice bowl stretches into the collecting box body, and carbon dioxide gas is released to anaesthetize the phytoseiid Chilo Persici;
the through hole is detachably covered with a sealing cover so as to seal the through hole.
Preferably, the lamp further comprises an illumination assembly, the illumination assembly comprises at least two groups of lamp holders and at least two lamp tubes, each group of lamp holders comprises two split lamp holders, and the two split lamp holders are respectively arranged on the inner wall surface of the collection box body and positioned at the upper part of the inner space of the collection box body; and two ends of each lamp tube are respectively arranged in the two split lamp holders of each group of lamp holders so as to provide illumination for the inside of the collecting box body.
Preferably, the collecting box further comprises a sealing glove for an operator to stretch into the collecting box for operation, at least two operation holes are formed in the side wall of the collecting box, and one end of the sealing glove is connected with the operation holes in a sealing mode.
Preferably, the bottom of the collecting box body is provided with four supporting rods, and the four supporting rods are respectively arranged at four corners of the bottom of the collecting box body.
The invention provides a method and a device for collecting phytoseiid mites for industrially producing living bodies, wherein the main structure of the collecting device is a transparent rectangular collecting box body, the interior of the collecting box body is of a cavity structure so as to place a host plant for breeding phytoseiid mites, one side wall of the collecting box body is designed to be of an open surface structure, a sliding groove is arranged on the inner side wall of the open surface close to the side edge, a movable sealing door is embedded in the sliding groove and slides up and down along the sliding groove so as to open or close the collecting box body, and a sealing gasket strip is arranged in the sliding groove so as to seal; after the host plant bred with the phytoseiid persimmons is placed in the collection box body, a dry ice bowl is placed on a bottom plate of the anesthetic support assembly through a through hole at the top of the collection box body, a support spring is pulled downwards under the gravity action of the dry ice bowl, so that the dry ice bowl extends into the collection box body, the through hole is tightly sealed and covered by a sealing cover, the dry ice in the dry ice bowl is gasified into low-temperature carbon dioxide, and the low-temperature carbon dioxide flows from top to bottom in the collection; during the anesthesia process of phytoseiid persimmons, the internal and external air pressures of the collecting box body are balanced through a vent valve arranged on the movable sealing door; the phytoseiid persimmons after anesthesia is sucked out to a wire mesh bag through a small dust collector arranged at the bottom of the collecting box body so as to be metered and subpackaged, and fresh air is blown into the metered and subpackaged phytoseiid persimmons in the guiding-in wind tunnel type reviving box for reviving.
Drawings
Fig. 1 is a schematic perspective view of a collecting device for industrially producing living phytoseiid persicae;
FIG. 2 is a perspective view of FIG. 1;
fig. 3 is a schematic structural view of the anesthetic support assembly of fig. 1 and 2.
In the figure: the insect pest trapping device comprises a collecting box body 1, a supporting rod 2, a movable sealing door 3, a handle 4, a sliding chute 5, a lamp holder 6, a lamp tube 7, an operation hole 8, a vent valve 9, an insect pest sucking hose 10, a small dust collector 11, a through hole 12, a supporting spring 13, a guide cylinder 14, a supporting connecting plate 15, a bottom plate 16, a supporting sleeve 17 and a sealing cover 18.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.
In the description of the present invention, it is to be understood that the terms "central," "axial," "radial," "longitudinal," "transverse," "length," "width," "upper," "lower," "front," "rear," "left," "right," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "vertical," "horizontal," and the like are used in the orientations and positional relationships indicated in the drawings to facilitate the description of the invention and to simplify the description, but do not indicate or imply that the device or element so referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be considered as limiting the invention.
In the present invention, unless otherwise expressly stated or limited, the first feature being "on" or "under" the second feature may comprise the first and second features being in direct contact, or the first and second features being in contact, not directly, but via another feature therebetween. Also, the first feature being "on," "above" and "over" the second feature includes the first feature being directly above and obliquely above the second feature, or merely indicating that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature includes the first feature being directly under and obliquely below the second feature, or simply meaning that the first feature is at a lesser elevation than the second feature.
Referring to fig. 1 to 3, fig. 1 is a schematic perspective view of an industrial collection device for phytoseiid persimilis according to an embodiment of the present invention; FIG. 2 is a perspective view of FIG. 1; fig. 3 is a schematic structural view of the anesthetic support assembly of fig. 1 and 2.
The invention provides a method for collecting phytoseiid mites for industrially producing living bodies, which comprises the steps of placing host plants bred with phytoseiid mites into a collection box, introducing anesthetic gas into the collection box to anesthetize the phytoseiid mites, sucking the anesthetized phytoseiid mites into a wire mesh bag through a small dust collector communicated with the collection box, metering and subpackaging the phytoseiid mites in the wire mesh bag, and then placing the metered and subpackaged phytoseiid mites into a wind tunnel type reviving box to blow in fresh air for reviving.
In one embodiment of the invention, the anesthetic gas is nitrogen or carbon dioxide.
The invention also provides a collecting device for industrially producing the living phytoseiid mites, which comprises a transparent collecting box body 1, an anesthetic supporting component and an insect body separating component;
the interior of the collection box body 1 is a cavity structure, and a host plant for breeding phytoseiulus persimilis can be placed in the cavity structure;
the collecting box body 1 is a sealed box body with only an opening on the front side surface, and a movable sealing door 3 is arranged on the front side surface of the collecting box body 1 and is used for opening or closing the collecting box body 1;
the top wall of the collection box body 1 is provided with at least two through holes 12 for placing dry ice bowls into the collection box body 1;
the anesthetic support assembly comprises a dry ice bowl for storing solid anesthetic dry ice and a dry ice bowl support frame for supporting the dry ice bowl in the collection box body 1, the dry ice bowl support frame is fixedly arranged on the inner wall surface of the collection box body 1 and is positioned at the upper part of the inner space of the collection box body 1, the dry ice bowl is placed on the dry ice bowl support frame so that low-temperature carbon dioxide gas is generated after the dry ice is gasified, and the low-temperature carbon dioxide gas flows from top to bottom in the collection box body 1 so as to be used for anesthetizing the amblyseius persicae;
the worm body separating component comprises a wire mesh bag, a worm body suction hose 10 and a small dust collector 11;
the polypide suction hose 10 is arranged at the bottom of the collection box body 1, the upper end of the polypide suction hose penetrates through the bottom wall of the collection box body 1 and extends into the collection box body 1, the lower end of the polypide suction hose 10 is communicated with a suction opening of the small dust collector 11, the wire mesh bag is arranged at an inlet of the polypide suction hose 10, and the phytoseiid persimmons in the collection box body 1 are sucked into the wire mesh bag through negative pressure generated at the pipe opening of the polypide suction hose 10 so as to measure and divide the phytoseiid persimmons in the wire mesh bag; the loaded phytoseiid persimmons are put into a reviving box arranged outside and revived by blowing in fresh air.
In one embodiment of the invention, a chute 5 for embedding the movable sealing door is arranged on the edge of the opening of the collecting box body 1; the movable sealing door 3 is embedded in the sliding chute 5 and can freely slide up and down along the sliding chute 5 so as to open or close the collecting box body 1; and a sealing gasket strip for sealing is arranged in the sliding groove 5.
In one embodiment of the invention, a handle 4 is fixedly arranged at the top of the movable sealing door 3, and a holding space is arranged in the handle 4 for an operator to pull the movable sealing door 3 to slide up and down through the handle 4.
In one embodiment of the present invention, the dry ice collecting device further comprises an air pressure balancing assembly, the air pressure balancing assembly comprises a vent valve 9, the vent valve 9 is arranged on the movable sealing door 3 and is located at the top of the movable sealing door 3, and one end of the vent valve 9 is communicated with the internal cavity structure of the collecting box body 1 so as to balance the air pressure inside and outside the collecting box body 1 after the dry ice is gasified.
In one embodiment of the present invention, the anesthetic support assembly includes a support spring 13, a guide cylinder 14, a support connection plate 15, a bottom plate 16 and a support sleeve 17;
the upper end of the supporting spring 13 is fixedly connected to the inner wall of the top plate of the collecting box body 1 and is positioned at the side part of the through hole 12, the supporting spring 13 extends downwards, and the lower end of the supporting spring is fixedly connected to the supporting connecting plate 15;
the supporting connection plate 15 is arranged at the side of the bottom plate 16 and extends outwards;
the guide cylinder 14 is arranged on the support connecting plate 15, and the lower end of the support spring 13 extends downwards into the guide cylinder 14 and is fixedly connected to the support connecting plate 15 so as to be guided and limited by the guide cylinder 14;
the supporting sleeve 17 is arranged on the bottom plate 16 and extends upwards along the side edge of the bottom plate 16, so that an installation space is formed on the bottom plate 16;
an external dry ice bowl is placed in the installation space on the bottom plate 16 through the through hole 12, and the supporting spring 13 is stretched downwards under the action of the gravity of the dry ice bowl, so that the dry ice bowl extends into the collection box body 1, and carbon dioxide gas is released to anaesthetize phytoseiulus persimilis;
the through hole 12 is detachably covered with a sealing cover 18 to seal the through hole 12.
In an embodiment of the present invention, the present invention further comprises an illumination assembly, the illumination assembly comprises at least two groups of lamp holders 6 and at least two lamp tubes 7, each group of lamp holders 6 comprises two split lamp holders 6, and the two split lamp holders 6 are respectively disposed on the inner wall surface of the collection box 1 and located at the upper portion of the inner space of the collection box; two ends of each lamp tube 7 are respectively arranged in the two split lamp holders of each group of lamp holders 6.
In an embodiment of the invention, the phytoseiid persicae industrial collection device further comprises a sealing glove for an operator to enter the collection box body 1 for operation, at least two operation holes 8 are formed in the side wall of the collection box body 1, and one end of the sealing glove is connected with the operation holes in a sealing manner.
In one embodiment of the present invention, four support rods 2 are disposed at the bottom of the collecting box 1, and the four support rods 2 are disposed at four corners of the bottom of the collecting box 1.
Furthermore, the right side surface of the collecting box body 1 is provided with a sealing glove, the sealing glove can be flexibly operated in the collecting box body 1 when being worn, and the sealing requirement can be ensured; the front side surface is provided with a movable sealing door 3 which can directly put the host plant which is bred with phytoseiulus persimilis into the collection box body 1; then a dry ice bowl filled with dry ice is placed in the collection box body, low-temperature carbon dioxide gas is generated after the dry ice is gasified, and the low-temperature carbon dioxide gas flows from top to bottom in the collection box body 1 for anaesthetizing the phytoseiid persimmons; the upper part of the movable sealing door 3 is provided with an openable vent valve 9 which is opened when the anesthetic gas is introduced for balancing the internal and external air pressure of the collection box body and exhausting the air in the box body, and the vent valve 9 and other openings such as a small-sized dust collector 11 are sealed after the ventilation is finished so as to avoid the anesthetic gas from escaping; the upper side is provided with an illuminating lamp tube 7 for illuminating during operation.
The source of anesthetic gas in the present invention has a variety of options, not limited to the following:
A. nitrogen or carbon dioxide cylinders: supplying gas into the collection box body 1 through an anesthetic gas hose;
B. and (3) dry ice, namely, a dry ice bowl 15 with porous metal is hoisted on the upper part and the upper side surface of the collection box body 1, a certain volume of dry ice is placed in the dry ice bowl 15, all openings except the vent valve 9 on the upper part of the movable sealing door 3 are closed, the internal and external air pressure is balanced, the air in the box is discharged, and all the openings are closed after the dry ice is gasified. The usage amount of the anesthetic gas is enough to immobilize the phytoseiid persiiid mite living body, long-time anesthesia is not needed, and after the package is finished, all insects can revive through transient recovery.
The phytoseiid mite separating and metering tool comprises the following parts:
A. the separation tool has a plurality of options, not limited to the following options:
1. separating and screening: selecting the size of the sieve pores of the separating sieve according to the size of the phytoseiid persimilis, shearing the host plant fed with phytoseiid persimilis, pouring into the separating sieve, sieving out polypide, and connecting the phytoseiid persimilis with a tray;
2. small vacuum cleaner 11: set up small-size dust catcher 11 in the bottom outside of collecting box 1, fill in the silk screen bag in the import department of polypide suction hose 10, for example can use a short silk socks to act as the silk screen bag here to strangle with the rubber band, regulate and control small-size dust catcher 11 dynamics, as long as can inhale the silk screen bag with the polypide.
B. The metering tool has a plurality of options, not limited to the following options:
1. a weighing tool: for example, the relationship between the weight of the population of the polypide and the number of the polypide is calculated in advance, and a relational expression between the weight of the population of the polypide and the number of the polypide is made. Reversely deducing the number of the polypide according to the group weight, and subpackaging;
2. volume measurement tool: for example, a measuring cylinder, the relationship between the population volume of the polypide and the polypide number is calculated in advance, and a relational expression between the population volume of the polypide and the polypide number is made; and (4) reversely deducing the number of the polypide according to the volume of the colony, and then subpackaging.
The reviving box is used for quickly inputting fresh air to revive phytoseiid mites; there are a number of options, not limited to the following:
A. wind tunnel type reviving box: and (3) putting the packaged phytoseiid persimmons into the wind tunnel type reviving box, wherein two ends of the wind tunnel type reviving box are opened, and one end of the wind tunnel type reviving box is used for supplying air to the fan until all phytoseiid persimmons are revived completely.
For further understanding of the present invention, the following examples are provided to illustrate the collecting method of phytoseiulus persimilis for industrially producing living phytoseiulus persimilis, and the scope of the present invention is not limited by the following examples.
Taking collection of living phytoseiid persicae as an example, three batches of phytoseiid persicae with the same batch, feeding condition and feeding amount are taken, and the insect living bodies are collected by adopting the method provided by the invention and the traditional collection method.
Example 1
Putting a batch of phytoseiid persimmons together with the feeding host leaves into a collection box body 1, introducing anesthetic gas carbon dioxide for 1min, then closing an anesthetic gas source gas valve, standing for 3min, after about 90% of the phytoseiid persimmons are anesthetized, using a small dust collector sleeved with silk stockings to use the lowest gear, sucking the worm bodies into the silk stockings to directly suck the worm bodies, weighing and metering the worm bodies, subpackaging according to the packaging requirement, and standing in a wind tunnel; in this example, the living phytoseiid mites were collected, the collection rate was 92.77%, the survival rate was 98.87% immediately after collection, and the survival rate was 90.17% immediately after 1 day after collection.
Comparative example 1
Directly brushing polypseiulus persimilis in a tray from feeding host leaves by using a soft brush without anesthesia, and subpackaging according to packaging requirements after simple metering, wherein the operation process needs to be as fast as possible, and the escape of the polypseiulus persimilis is reduced; in this comparative example, the living phytoseiid mites were collected, the collection rate was 72.47%, the survival rate was 65.83% immediately after collection, and the survival rate was 34.98% immediately after collection for 1 day.
Comparative example 2
Directly placing phytoseiid persimmons and feeding host leaves into one end of a collecting container without anesthesia, allowing the phytoseiid persimmons and the feeding host leaves to move, collecting the polypide at the other end of the collecting container every 30min for 4h, weighing, and subpackaging according to the packaging requirement; in this comparative example, the living phytoseiid mites were collected, the collection rate was 81.23%, the survival rate was 99.14% immediately after collection, and the survival rate was 92.31% immediately after 1d after collection.
Comparing the example 1, the comparative example 1 and the comparative example 2, it can be seen that the method provided by the invention and the method in the comparative example 1 are short in the time for collecting phytoseiid mites, can be completed within 30-60min, the method in the comparative example 2 is relatively long in the time, and the collection rate is more than 80% and at least needs 4-5 h; by using the method provided by the invention and the method in the comparative example 2, the phytoseiid persicae collection does not worry about a large amount of phytoseiid persicae escaping in the operation process, when the method in the comparative example 1 is used, the phytoseiid persicae does not need anesthesia treatment, the escaping phenomenon is serious after being disturbed, and the operator can be in a busy hand and foot; the collection rate of the collection method provided by the invention is 20.30 percent and 11.54 percent higher than that of the collection method in comparative example 1 and comparative example 2 respectively; by using the method provided by the invention and the method in the comparative example 2, the phytoseiid persicae has less death injury, the survival rates of the phytoseiid persicae just after collection are respectively 98.87% and 99.14%, and the survival rates after 1d are still as high as 90.17% and 92.31%, while the method in the comparative example 1 is very easy to cause the phytoseiid persicae death injury, the survival rate is 65.83% just after collection, and the survival rate after 1d is reduced to 34.98%. Therefore, the invention provides the method and the device for industrially collecting phytoseiid persicae with higher collection efficiency, less escape and lower mortality.
The invention provides a plurality of technical problems and simultaneously provides a corresponding technical scheme aiming at each technical problem. The technical problems are not independent of each other but are mutually influenced, so that the technical effects obtained by solving the corresponding technical problems can be obviously improved or the technical problems can be solved simultaneously by combining the technical solutions with other technical solutions on the basis of solving the corresponding technical problems. On the basis that each single technical scheme solves the corresponding technical problem, a plurality of progressive technical schemes are combined and overlapped with each other, the technical schemes are matched with each other and mutually promoted to form an integral scheme, the obtained technical effect is far better than that of any one of the technical schemes, and the overlapping effect is obvious.
Methods and devices not described in detail in the present invention are all the prior art and are not described in detail.
The principles and embodiments of the present invention are explained herein using specific examples, which are set forth only to help understand the method and its core ideas of the present invention. It should be noted that, for those skilled in the art, it is possible to make various improvements and modifications to the present invention without departing from the principle of the present invention, and those improvements and modifications also fall within the scope of the claims of the present invention.
Claims (7)
1. A collecting device for realizing a collecting method of living phytoseiid mites for industrialized production is characterized by comprising a transparent collecting box body (1), an anesthetic supporting component and an insect body separating component;
the interior of the collection box body (1) is of a cavity structure, and a host plant for breeding phytoseiid persimmons persicae can be placed in the collection box body;
the collecting box body (1) is a sealed box body with an opening only on the front side surface, and a movable sealing door (3) is arranged on the front side surface of the collecting box body (1) and used for opening or closing the collecting box body (1);
the top wall of the collection box body (1) is provided with at least two through holes (12) for placing dry ice bowls into the collection box body (1);
the anesthetic support assembly comprises a dry ice bowl for storing solid anesthetic dry ice and a dry ice bowl support frame for supporting the dry ice bowl in the collection box body (1), the dry ice bowl support frame is fixedly arranged on the inner wall surface of the collection box body (1) and is positioned on the upper portion of the inner space of the collection box body (1), the dry ice bowl is placed on the dry ice bowl support frame so that low-temperature carbon dioxide gas is generated after the dry ice is gasified, and the low-temperature carbon dioxide gas flows from top to bottom in the collection box body (1) so as to be used for anesthetizing the amblyseius spinosus plantinum;
the anesthetic support assembly further comprises a support spring (13), a guide cylinder (14), a support connecting plate (15), a bottom plate (16) and a support sleeve (17);
the upper end of the supporting spring (13) is fixedly connected to the inner wall of the top plate of the collecting box body (1) and is positioned at the side part of the through hole (12), the supporting spring (13) extends downwards, and the lower end of the supporting spring is fixedly connected to the supporting connecting plate (15);
the supporting connecting plate (15) is arranged on the side part of the bottom plate (16) and extends outwards;
the guide cylinder (14) is arranged on the supporting connecting plate (15), and the lower end of the supporting spring (13) extends downwards into the guide cylinder (14) and is fixedly connected to the supporting connecting plate (15) so as to be guided and limited by the guide cylinder (14);
the supporting sleeve (17) is arranged on the bottom plate (16) and extends upwards along the side edge of the bottom plate (16), so that an installation space is formed on the bottom plate (16);
an external dry ice bowl is placed in the installation space on the bottom plate (16) through the through hole (12), and the supporting spring (13) stretches downwards under the action of the gravity of the dry ice bowl, so that the dry ice bowl extends into the collection box body (1) to release carbon dioxide gas to anaesthetize the phytoseiidae persicae persimmons;
a sealing cover (18) is detachably covered on the through hole (12) so as to seal the through hole (12);
the worm body separating component comprises a wire mesh bag, a worm body suction hose (10) and a small dust collector (11);
polypide suction hose (10) set up in collect the bottom of box (1), just the upper end of polypide suction hose runs through the diapire of collecting box (1) stretches into in the collection box (1), polypide suction hose (10) the lower extreme with the suction opening intercommunication of small-size dust catcher (11), the silk screen bag set up in the import department of polypide suction hose (10) to be used for the separation to collect the little phytoseiulus persicae of intelligence after the anesthesia in collection box (1).
2. The collecting device according to claim 1, characterized in that a chute (5) for embedding the movable sealing door is arranged on the edge of the opening of the collecting box body (1); the movable sealing door (3) is embedded in the sliding chute (5) and can freely slide up and down along the sliding chute (5) so as to open or close the collecting box body (1); and a sealing gasket strip for sealing is arranged in the sliding groove (5).
3. The collecting device according to claim 2, characterized in that a handle (4) is fixedly arranged on the top of the movable sealing door (3), and a holding space is arranged in the handle (4) for an operator to pull the movable sealing door (3) to slide up and down through the handle (4).
4. A collecting device according to claim 3, characterized by further comprising an air pressure balancing assembly, said air pressure balancing assembly comprising a vent valve (9), said vent valve (9) being arranged on the movable sealing door (3) and being located at the top of said movable sealing door (3), one end of said vent valve (9) being in structural communication with the internal cavity of said collecting bin (1) for balancing the air pressure inside and outside said collecting bin (1) after the dry ice has gasified.
5. The collecting device according to claim 4, further comprising an illuminating assembly, wherein the illuminating assembly comprises at least two groups of lamp holders (6) and at least two lamp tubes (7), each group of lamp holders (6) comprises two split lamp holders (6), and the two split lamp holders (6) are respectively arranged on the inner wall surface of the collecting box body (1) and positioned at the upper part of the inner space of the collecting box body; two ends of each lamp tube (7) are respectively arranged in two split lamp holders of each group of lamp holders (6) to provide illumination for the interior of the collection box body (1).
6. The collecting device according to claim 5, characterized by further comprising a sealing glove for an operator to enter the collecting box body (1) for operation, wherein at least two operation holes (8) are formed on the side wall of the collecting box body (1), and one end of the sealing glove is connected with the operation holes in a sealing manner.
7. The collecting device according to claim 6, characterized in that the bottom of the collecting box body (1) is provided with four supporting rods (2), and the four supporting rods (2) are respectively arranged at the four corners of the bottom of the collecting box body (1).
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| CN115281158B (en) * | 2022-08-15 | 2023-09-15 | 绵阳市农业科学研究院 | Method for large-scale propagation of phytoseiid chile |
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| CN102640734A (en) * | 2012-05-17 | 2012-08-22 | 环境保护部南京环境科学研究所 | Method for carrying out bee colony anaesthesia by utilizing bee honeycomb anaesthesia device |
| CN104396889A (en) * | 2014-10-27 | 2015-03-11 | 四川农业大学 | Neoseiulus californicus low-temperature storage method |
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| JP3547174B2 (en) * | 1994-07-28 | 2004-07-28 | 農薬バイオテクノロジー開発技術研究組合 | How to save mites |
| WO2010127787A2 (en) * | 2009-05-07 | 2010-11-11 | Bayer Cropscience Ag | Active ingredient combinations having insecticidal and acaricidal properties |
| CN202842152U (en) * | 2012-11-01 | 2013-04-03 | 天津市植物保护研究所 | Phyoseiulus persimilis separating and collecting implement |
| CN204860700U (en) * | 2015-08-05 | 2015-12-16 | 中国农业科学院草原研究所 | Open -air insect collection device |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102640734A (en) * | 2012-05-17 | 2012-08-22 | 环境保护部南京环境科学研究所 | Method for carrying out bee colony anaesthesia by utilizing bee honeycomb anaesthesia device |
| CN104396889A (en) * | 2014-10-27 | 2015-03-11 | 四川农业大学 | Neoseiulus californicus low-temperature storage method |
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