CN106613484B - A kind of mating system of anti-seasonal febrile diseases pepper seedling - Google Patents

A kind of mating system of anti-seasonal febrile diseases pepper seedling Download PDF

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Publication number
CN106613484B
CN106613484B CN201611218199.0A CN201611218199A CN106613484B CN 106613484 B CN106613484 B CN 106613484B CN 201611218199 A CN201611218199 A CN 201611218199A CN 106613484 B CN106613484 B CN 106613484B
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scion
pepper
grafting
seedling
stock
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CN106613484A (en
Inventor
郝朝运
胡丽松
黄大雄
范睿
谭乐和
吴刚
杨建峰
李付鹏
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Spice and Beverage Research Institute of Chinese Academy of Tropical Agricultural Sciences
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Spice and Beverage Research Institute of Chinese Academy of Tropical Agricultural Sciences
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00Vegetative propagation
    • A01G2/30Grafting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G9/00Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
    • A01G9/02Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
    • A01G9/029Receptacles for seedlings
    • A01G9/0299Handling or transporting of soil blocks or seedlings

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Soil Sciences (AREA)
  • Catching Or Destruction (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The present invention relates to agricultural technology field, discloses a kind of mating system of anti-seasonal febrile diseases pepper seedling.Mating system of the present invention first prepares the scion of pepper to be grafted;Then by the scion and stock grafting in a manner of oblique side grafting;Seedling is managed after grafting, spike length waiting goes out cut anvil after sprouting, obtains pepper grafting seedling.The present invention is in the grafting procedures to pepper, with Chinese unique wheat PIPER FLAVIFLORUM (Piper flaviflorum) for stock, it is oblique side grafting mode to change conventional cleft grafting mode, spike length waiting goes out after sprouting cut anvil again, significantly improve the survival rate of pepper grafting seedling, and realize that whole grafting seedlings possess the purpose of anti-seasonal febrile diseases ability, can effectively resist the infringement of pepper seasonal febrile diseases, improve the benefit of pepper plantation industry.

Description

A kind of mating system of anti-seasonal febrile diseases pepper seedling
Technical field
The present invention relates to agricultural technology field, and in particular to a kind of mating system of anti-seasonal febrile diseases pepper seedling.
Background technology
Pepper (Piper nigrum) is that the perennial woody climber of Piperaceae (Piperaceae) Piper (Piper) is planted Thing, its fruit have pungent note, are known as the good reputation of " king of spices ", are the most important spice crops in the world, have medicine again With value and industrial utility value.At present, pepper is throughout Southeast Asia, Africa, Hispanic nearly 20 countries and regions, harvest Area 5.53 × 105hm2, total output 4.33 × 105T, wherein China pepper are mainly distributed on Hainan, Yunnan, Guangdong and Guangxi etc. Provinces and regions, annual production 3.6 × 104T, occupy the 5th, the world.By popular flavouring and there is extensive use as a kind of depth Tropical agricultural product, pepper exist for a long time it is in great demand with insufficient problem, as people's living standard improves and drinks Food structure constantly changes, and China will also continue to increase to the demand of pepper, and prospect of industrial development is wide.
The susceptible pepper seasonal febrile diseases of pepper cultivar, it is the first major disease in production, limits the benign development of industry.Recklessly Green pepper seasonal febrile diseases are caused by phytophthora blight of pepper (Phytophthora capsici Leon), belong to a kind of weather rely on, contagiousness it is very strong Soil-borne disease, still take in production put prevention first, the measure supplemented by integrated control, depend on chemical disinfectant, greatly Plant development cost is added greatly, and easily causes environmental pollution.Pepper breed breeding is with a long history, but due to cultigen lack it is anti- Seasonal febrile diseases gene, and the digging utilization of wild relatives disease-resistant gene is limited by biological characteristics and technical merit, therefore it is international Not yet cultivate effective disease-resistant varieties.
At present, China pepper main breed is that heat draws No. 1 (P.nigrum c.v.Reyin-1, being commonly called as Indonesia's large leaf), Area coverage is planted more than 90%, belongs to susceptible seasonal febrile diseases kind, great amount of cost need to be put into and be used for seasonal febrile diseases prevention and control.Therefore, pepper resists Seasonal febrile diseases seedling fostering has turned into current industry key issue urgently to be resolved hurrily.Seedling is cultivated using heteroplastic graft method, can be effective Improve the disease resistance of grafting seedling.At present, the engrafting method used both at home and abroad in report is generally from stock for South America tree pepper (Piper colubrinum), betel (Piper betle) etc., graft seedling growth, but the pepper cultivated are carried out with cleft graft Seedling can not obtain anti-seasonal febrile diseases ability, and graft survival rate is not high.Such as patent CN103283495A, it discloses a kind of pepper with Piper wild species betel makes the pepper seedling of institute's grafting improve resistance, but do not obtain with the technical scheme of cleft graft grafting The ability of seasonal febrile diseases must be resisted, while its graft survival rate is up to just to 80%.There is not also one kind to be suitable for the anti-pest of pepper at present The mating system of disease seedling.
The content of the invention
In view of this, it is an object of the invention to provide a kind of mating system of pepper seedling so that the mating system Graft survival rate be significantly improved.
Another object of the present invention is to provide a kind of mating system of anti-seasonal febrile diseases pepper seedling so that the Hu of acquisition Green pepper seedling possesses anti-seasonal febrile diseases ability.
To achieve these goals, the present invention provides following technical scheme:
A kind of mating system of anti-seasonal febrile diseases pepper seedling, including:
Step 1, the scion for preparing pepper to be grafted;
Step 2, by the scion and stock grafting in a manner of oblique side grafting;
Grafting is managed after step 3, grafting, spike length waiting goes out cut anvil after sprouting, obtains anti-seasonal febrile diseases pepper grafting Miao Miao.
Transferred for existing pepper grafting technique in the defects of engrafting method, the present invention by way of oblique side grafting Connect, the pepper seedling graft survival rate obtained is higher.On this basis, the present invention preferably PIPER FLAVIFLORUM (Piper Flaviflorum stock) is used as, pepper grafting is obtained anti-seasonal febrile diseases ability.
Wherein, preferably, step 1 is:
1-2 raw, stalwartness, the main stem having no mechanical damage are chosen from pepper tree to be grafted;Leave and take main stem diameter 0.5- 1.0cm parts, long 6-10cm scion is cut into, scion has 1-2 section and 1-2 axillary bud, cuts off blade, retains petiole and armpit Bud;A long 1-1.6cm long bevel is first cut in scion lower end, end is as deep as the thick 1/2-2/3 of scion, then at the phase of long bevel A long 0.7-1.2cm short bevel is cut on opposite, obtains pepper scion to be grafted (scion schematic diagram is shown in Fig. 1).Meanwhile in order to obtain The more preferable scion of state is obtained, 20-30d fleshes up 1 time using growth-promoting before scion is cut.Cut and spray absorbability within 1 week before scion and kill Microbial inoculum 1 time.
In the specific embodiment of the invention, the pepper tree to be grafted is that 3-5 is raw, growth is normal and no disease and pests harm shadow Loud pepper plant, and the pepper to be grafted is drawn No. 1 (Piper nigrum c.v.Reyin-1) for heat.
Preferably, step 2 is:
Daily mean temperature is not less than 25 DEG C between grafting time, and in the morning 7:00-9:00 or afternoon 16:00-17:30 are carried out; Stock, as grafting part, cuts a flat angular cut, as deep as xylem 1/3-1/2, length and scion from the native more straight places of face 20cm Long bevel is equal;Scion is embedded in stock otch, scion forming layer is aligned, touched with stock forming layer, from bottom to top by scion Packed tightly with stock junction with plastic sheeting.Meanwhile in order to obtain the more preferable stock of state, 20-30d applies to stock before grafting It is fertile 1 time with liquid bio;Spray systemic fungicide 1 time within 1 week before grafting, and be filled with enough bottom water.
In the specific embodiment of the invention, the stock is 2-3 lifes, main stem suberification length more than 40cm, growth The seedling that normal and no disease and pests harm influences.
Can conventionally it be carried out for the seed and seedling management after grafting, the present invention gives preferably square in step 3 Formula:After grafting, keep seedling 30%-50% to cover, treat that scion is healed with stock, cut off scion axillary bud plastic sheeting nearby, treat Scion grows Section 2 sprouting, in the cut anvil at the top 10cm of grafting part, obtains pepper seedling.
The present invention carries out grafting with oblique side grafting and cleft grafting two ways to pepper respectively, as a result shows, in a manner of oblique side grafting The pepper seedling that grafting obtains, average survival reach 87.7%, and highest survival rate is 93.3%, compared to existing patent The survival rate of highest 80% in CN103283495A, engrafting method of the present invention can significantly improve the survival rate of pepper seedling.Together When, in order to increase comparability, the present invention carries out grafting respectively using betel and PIPER FLAVIFLORUM as stock using cleft grafting mode, Select PIPER FLAVIFLORUM for stock when, the pepper seedling obtained in a manner of oblique side grafting all possesses anti-seasonal febrile diseases ability, and with cleft grafting side Seedling that formula obtains and it is not all possess anti-seasonal febrile diseases ability, and using betel as the not anti-seasonal febrile diseases energy of the pepper seedling that stock obtains Power, while be not so good as using oblique side grafting mode on graft survival rate.
From above technical scheme, the present invention is in the grafting procedures to pepper, using PIPER FLAVIFLORUM as stock, changes normal Cleft grafting mode is oblique side grafting mode, significantly improves the survival rate of pepper seedling, while selects chrysanthemum on this basis recklessly Green pepper is stock, and the purpose that whole seedlings possess anti-seasonal febrile diseases ability can be achieved, can effectively resist the infringement of pepper seasonal febrile diseases, improve recklessly Green pepper plants the benefit of industry.
Brief description of the drawings
Fig. 1 show scion schematic diagram;Wherein A is the long bevel side schematic diagram of scion, and B is short bevel side schematic diagram, and C is to connect The side view of fringe side view, i.e., long bevel and short bevel.
Embodiment
The invention discloses a kind of mating system of anti-seasonal febrile diseases pepper seedling, those skilled in the art can be used for reference in this paper Hold, be suitably modified technological parameter realization.In particular, all similar replacements and change are to those skilled in the art For be it will be apparent that they are considered as being included in the present invention.Mating system of the present invention is by preferably implementing Example is described, and related personnel can substantially not depart from present invention, method described herein entered in spirit and scope Row change is suitably changed with combining, to realize and using the technology of the present invention.
Just a kind of mating system of anti-seasonal febrile diseases pepper seedling provided by the present invention is described further below.
Embodiment 1:Mating system of the present invention
Selection life in 5 years, growth is normal and the heat of no disease and pests harm influence draws No. 1 and is used as scion elite stand;Before scion is cut 25d, fleshed up 1 time using growth-promoting;Systemic fungicide is sprayed within 1 week before cutting scion 1 time.
2 months 2012, select life in 2 years, main stem suberification length 50cm, grow the chrysanthemum Hu that normal and no disease and pests harm influences Green pepper seedling is as stock, 30d applicating liquids bio-fertilizer 1 time before grafting;Spray systemic fungicide 1 time within 1 week before grafting, And it is filled with enough bottom water.
(25.6 DEG C of the daily mean temperature) morning 7 on April 10th, 2012:30,2 years lifes, stalwartness, nothings are chosen from scion elite stand The main stem of mechanical damage;Diameter is chosen between 0.5-1.0cm parts, is cut into 7cm scion, each scion has 1 section, 1 leaf Bud;Blade is cut off, retains axillary bud, petiole;First cut a long 1.5cm long bevel in scion lower end, end as deep as fringe it is thick 2/ 3, a long 0.8cm short bevel is cut then at the back side.
Stock main stem surface keeps drying before bud connects;In stock from the native more straight places of face 21cm as grafting part;Cut one Flat angular cut, as deep as xylem 1/3, length is equal with long bevel.
Using oblique side grafting method, the scion cut is embedded in stock otch, scion forming layer is aligned with stock forming layer, is close Connect;Scion and stock junction are packed tightly with plastic sheeting from bottom to top.
Seedling 30%-50% is kept to cover after grafting;On May 10th, 2012, scion are healed with stock, cut off scion axillary bud Neighbouring plastic sheeting;Scion on July 10 in 2012 grows Section 2 sprouting, in the cut anvil at the top 10cm of grafting part, obtains Pepper seedling.
Embodiment 2:Cleft grafting mode engrafting method
Selection life in 5 years, growth is normal and the heat of no disease and pests harm influence draws No. 1 and is used as scion elite stand;Before scion is cut 25d, fleshed up 1 time using growth-promoting;Systemic fungicide is sprayed within 1 week before cutting scion 1 time.
2 months 2012, select life in 2 years, main stem suberification length 50cm, growth is normal and the betel of no disease and pests harm influence is real Raw seedling is as stock, 30d applicating liquids bio-fertilizer 1 time before grafting;Spray systemic fungicide 1 time within 1 week before grafting, and fill Vola water.
(25.6 DEG C of the daily mean temperature) morning 7 on April 10th, 2012:30,2 years lifes, stalwartness, nothings are chosen from scion elite stand The main stem of mechanical damage;Diameter is chosen between 0.5-1.0cm parts, is cut into long 7cm scion, there is 1 to be saved for each scion, 1 Leaf bud;Blade is cut off, retains axillary bud, petiole;Two long 1.5cm bevel is symmetrically cut in scion lower end.
Stock main stem surface keeps drying before bud connects;Cut in stock from the native more straight places of face 21cm, in lower end grafting Position cut one it is symmetrical cut (vertical with existing patent CN103283495A rive identical), depth and bevel equal length.
The scion cut is embedded in stock otch, scion forming layer is aligned, touched with stock forming layer;From bottom to top will Scion and stock junction are packed tightly with plastic sheeting.
Seedling 30%-50% is kept to cover after grafting;On May 10th, 2012, scion are healed with stock, cut off scion axillary bud Neighbouring plastic sheeting;Scion on July 10 in 2012 grows Section 2 sprouting, in the cut anvil at the top 10cm of grafting part, obtains (in the present embodiment, except the difference that must have in technique, such as the making of scion bevel, stock otch, other need to keep pepper seedling Consistent experimental condition is in the same manner as in Example 1).
Embodiment 3:Graft survival rate and anti-seasonal febrile diseases ability contrast test
Grafting is carried out to pepper according to the method for embodiment 1 and embodiment 2 respectively, every kind of method carries out three groups simultaneously, often 30 plants of group, the Statistical Comparison of survival rate and seasonal febrile diseases resistance, knot are carried out to the pepper seedling finally obtained under identical test environment Fruit see the table below:
The Contrast on effect of different grafting methods after grafting 3 months
As can be known from Table 1, using PIPER FLAVIFLORUM as stock, using the oblique side grafting method seedling breeding method of the present invention, pepper Kind shoot survival percent average reachable more than 85%, significantly larger than conventional use of cleft grafting grafting, and the grafting height of seedling obtained resists Pepper seasonal febrile diseases.
Described above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should It is considered as protection scope of the present invention.

Claims (3)

  1. A kind of 1. mating system of anti-seasonal febrile diseases pepper seedling, it is characterised in that including:
    Step 1, the scion for preparing pepper to be grafted;
    Step 2, by the scion and stock grafting in a manner of oblique side grafting, the stock is PIPER FLAVIFLORUM;
    Seedling is managed after step 3, grafting, spike length waiting goes out cut anvil after sprouting, obtains anti-seasonal febrile diseases pepper seedling;
    Wherein, step 1 is specially:1-2 raw, stalwartness, the main stem having no mechanical damage are chosen from pepper plant to be grafted;Leave and take Main stem diameter 0.5-1.0cm parts, long 6-10cm scion is cut into, scion has 1-2 section and 1-2 axillary bud, cuts off blade, Retain petiole and axillary bud;A long 1-1.6cm long bevel is first cut in scion lower end, end as deep as the thick 1/2-2/3 of scion, then A long 0.7-1.2cm short bevel is cut in the opposite face of long bevel, obtains pepper scion to be grafted;The stock is 2-3 Raw, main stem suberification length exceedes that 40cm, growth be normal and the seedling of no disease and pests harm influence;
    Step 2 is specially:Daily mean temperature is not less than 25 DEG C between grafting time, and in the morning 7:00-9:00 or afternoon 16:00-17:30 Carry out;A flat angular cut is cut, as deep as xylem 1/3-1/2, length as grafting part from the native more straight places of face 20cm in stock It is equal with the long bevel of scion;Scion is embedded in stock otch, scion forming layer is aligned, touched with stock forming layer, from bottom to top Scion and stock junction are packed tightly with plastic sheeting;
    Step 3 is specially:After grafting, keep seedling 30%-50% to cover, treat that scion is healed with stock, it is attached to cut off scion axillary bud Nearly plastic sheeting, spike length waiting go out Section 2 sprouting, in the cut anvil at the top 10cm of grafting part, obtain anti-seasonal febrile diseases pepper kind Seedling.
  2. 2. mating system according to claim 1, it is characterised in that the pepper plant to be grafted is that 3-5 is raw, grown just Often and no disease and pests harm influences.
  3. 3. according to mating system described in claim 1-2 any one, it is characterised in that the pepper to be grafted is that heat draws No. 1.
CN201611218199.0A 2016-12-26 2016-12-26 A kind of mating system of anti-seasonal febrile diseases pepper seedling Active CN106613484B (en)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107691075B (en) * 2017-10-25 2020-02-04 中国热带农业科学院香料饮料研究所 Shading and flower controlling method for young peppers
CN108728513A (en) * 2018-07-12 2018-11-02 中国热带农业科学院香料饮料研究所 A kind of identification method of pepper germplasm seasonal febrile diseases resistance

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CN103283495B (en) * 2013-05-28 2015-01-14 云南省农业科学院热带亚热带经济作物研究所 Method for grafting pepernigrum L. and piperbetle L.
CN104115665B (en) * 2014-08-12 2016-01-06 宜宾云辰乔木园林有限责任公司 The blue root of a kind of yellow square rafter connects seedling-cultivating method
CN104542152A (en) * 2014-12-23 2015-04-29 贵州台金农业技术开发有限责任公司 Passion fruit planting method
CN105993629A (en) * 2016-06-30 2016-10-12 固镇县华丰农业有限公司 Grafting method for increasing eggplant yield

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Inventor after: Hao Chaoyun

Inventor after: Fan Rui

Inventor after: Hu Lisong

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Inventor after: Tan Lehe

Inventor after: Wu Gang

Inventor after: Yang Jianfeng

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