CN106561459A - Wild-imitating tissue culture planting method for dendrobium huoshanense - Google Patents
Wild-imitating tissue culture planting method for dendrobium huoshanense Download PDFInfo
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- CN106561459A CN106561459A CN201610990047.6A CN201610990047A CN106561459A CN 106561459 A CN106561459 A CN 106561459A CN 201610990047 A CN201610990047 A CN 201610990047A CN 106561459 A CN106561459 A CN 106561459A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/029—Receptacles for seedlings
- A01G9/0299—Handling or transporting of soil blocks or seedlings
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Abstract
The invention belongs to the technical field of dendrobe cultivation and discloses a wild-imitating tissue culture planting method for dendrobium huoshanense. The method comprises the following steps: seed and seedling cultivation, seed and seedling disinfection, construction of a planting ridged field, preparation of a cultivation medium, pavement of the cultivation medium, seed and seedling planting and cultivation management. Compared with the prior art, the cultivation method provided by the invention imitates the wild environment suitable for growth of dendrobium huoshanense, and meanwhile, a high-quality efficient fermenting cultivation medium is selected. Requirements on illumination, temperature, humidity, planting row spacing of planting are strictly controlled, so that the cultivated product is as same as a wild product in quality, and the output, the survival rate and the drying rate of dendrobium chrysanthum are obviously improved, and the incidence of diseases and pests is reduced. Meanwhile, the method further has the remarkable advantages of being convenient to operate, low in cultivation cost and the like.
Description
Technical field
The invention belongs to dendrobe cultivation technical field, and in particular to a kind of imitative wild tissue culture method for planting of Herba Dendrobii.
Background technology
Herba Dendrobii（Scientific name：Dendrobium nobile Lindl）, also known as celestial dry measure used in former times orchid rhythm, Radix Ophiopogonis, Herba Hylothelephii Verticillati, purple entangle celestial being
Strain, bracketplant, Lin Lan, prohibition of life, nobile flower etc..Stem is upright, and meat shape is plump, and slightly flat cylinder is long 10 ~ 60 centimetres, slightly up to 1.3
Centimetre.Medicinal plants, property slightly sweet flavor is micro- salty, trembles with fear, and returns stomach, kidney, lung meridian.Reinforcing stomach reg fluid, nourishing YIN and clearing away heat.For impairment of yin body fluid deficiency, mouth
Dry excessive thirst, lack of appetite is retched, after being ill deficiency-heat, poor vision.Herba Dendrobii flower appearance is graceful, and exquisite lovely, pattern is bright-coloured, fragrant odour, quilt
It is likened to one of " four view and admire greatly foreign flower ".
Herba Dendrobii is liked and grown in warm, moist, half cloudy half light environment, wet with more than 1000 millimeters of annual rainfall, air
Degree grows in the subtropical zones remote, thickly forested mountains more than 80%, 1 monthly mean temperature higher than 8 DEG C and is preferred, and clay fertilizer is required not very strictly, wild
Raw many growths on loose and thick bark or trunk, what is had is also grown in crack of stone.According to its habit, dendrobe cultivation ground
The environment of half cloudy half light is preferably selected, air humidity is more than 80%, and winter temperature is regional more than 0 DEG C.Human controllable's environment also can,
Seeds with Ammonium alum method, pear tree, Lignum cinnamomi camphorae etc. and should answer bark thickness to have the thick tree living of longitudinal furrow, aqueous many, branch and leaf cyclopentadienyl, trunk, stone
Ground also should should have moss growth and surface to have a small amount of humus in shady and cool, humid region on stone.
Herba Dendrobii is commonly called as a meter dry measure used in former times, is the herbaceous plant of orchid family Dendrobium.China national geography famous special product.Main product is in big
The An Weisheng Huoshan County in other mountain area, be grown in mostly between the overhanging cliff cliffstone gap that cloud and mist is curled up and old trees that reach into the skies on.To Jing
Often stay up late, with brain, tobacco and wine excessively, the weak crowd of body void, Jing often drinks and is particularly suitable for.Herba Dendrobii has function of improving eyesight, also has
Appearance, effect of health-care are stayed in YIN and YANG balance regulating, strengthening yang and invigorating kidney, skin care.Because the speed of growth is slow, yield poorly, the cycle it is long, Herba Dendrobii
Artificial growth slower development, market genuine piece difficulty is shown in.
The content of the invention
It is an object of the invention to provide a kind of low cost, the Herba Dendrobii that seedling early growth speed is fast, planting effect is good
Imitate wild tissue culture method for planting.
Specifically, the imitative wild tissue culture method for planting of Herba Dendrobii of the present invention, comprises the following steps：
（1）Seed cultivation：Using wild Herba Dendrobii mature fruit as explant, in being seeded to seedling culture base, it is in temperature
22-26 DEG C, intensity of illumination 2500-4000Lx, cultivate 60-80 days under conditions of light application time 8-12h/d, obtain Herba Dendrobii kind
Seedling；
（2）Seed and seedling disinfection：Herba Dendrobii seedling is taken, with 0.15-0.2% carbendazim solutions Herba Dendrobii seedling root 10- is soaked
12min；
（3）Build plantation embanked field：Site preparation, does growth 3.8-4.2m, width 0.9-1.1m, the embanked field of high 0.2-2.5m, between embanked field
Stay the aisle of wide 0.4-0.6m；
（4）It is prepared by cultivation matrix：In terms of parts by weight, by following raw materials according mixed fermentation 30-45d, cultivation matrix is obtained：Peat soil
15-22 parts, fertile soil 16-20 parts, yellow soil 12-16 parts, Cortex Pini ground product 10-15 parts, Cortex cinnamomi camphorae ground product 5-10 parts, jade
Rice bar ground product 5-10 parts, Bombyx mori L. 8-12 parts, chicken manure 8-12 parts；
（5）Cultivation matrix is laid：One layer of rubble is first laid on embanked field, the cultivation matrix of the high 6-9cm in a leafing embanked field is re-layed；
（6）Seedling is planted：By the Herba Dendrobii seedling dibbling after sterilization on cultivation matrix, and root water is poured in seedling root,
Seedling line-spacing is controlled to 8-12cm × 8-12cm, and plantation is killed using 15-20% Amicis after 7 days up to 1000 times of liquid of suspending agent
Bacterium；
（7）Plantation management：During plantation, the temperature for controlling to plant place is 20-28 DEG C, air humidity is 80-85%, shelters from heat or light
Degree 70-74%, intensity of illumination are 3500-7000Lx.
Peat soil belongs to mud coal also known as peat soil, black earth, fertile soil（peat）One kind.It is by Soaking environment
Vegetalitas remains（Such as algae, moss, aquatic and plant class）, it is deposited in a large number in stratum, under anaerobic status, Jing is micro-
The soil mineral that biological moieties decomposition is formed.Its rich in mineral substances and organic principle.
Peat is the long-term putrid fermentations of the residual leaf Jing of deadwood of topsoil trees in forest and is formed.It has diuretic moisturizing
The features such as fertilizer conservation, often material is planted with various organic or inorganics and be re-dubbed soil plant material to use.
Yellow soil is mainly made up of clay mineral, wherein based on hydromica class and montmorillonite, with quartz, Anhydrite, cloud
The mineral such as female and chlorite, simultaneously containing a certain amount of organic matter.
Rubble can be obtained directly from surrounding enviroment, and preferred Vermiculitum requires that particle diameter is 1.5-3cm.Vermiculitum be it is a kind of it is natural,
Nontoxic mineral, the dilatable mineral under high temperature action.It is a kind of rare mineral of comparison, belongs to silicate.It is brilliant
Body structure is monoclinic system, and it appears as Muscovitum from profile.Vermiculitum is produced when certain granite is hydrated.It is general
Produce simultaneously with asbestos.Because Vermiculitum has the ability of ion exchange, its nutrition to soil has greatly effect.
Further illustrate as the imitative wild tissue culture method for planting of Herba Dendrobii of the present invention, in step（4）In, institute
State Cortex Pini ground product be Cortex Pini is crushed after cross 20-40 mesh sieves be prepared；The Cortex cinnamomi camphorae ground product is by Cortex cinnamomi camphorae
Cross 20-40 mesh sieves after crushing to be prepared；The milled powders of cornstalk mince be corn stalk is crushed after cross 20-40 mesh sieves prepare and
.
Further illustrate as the imitative wild tissue culture method for planting of Herba Dendrobii of the present invention, in laying cultivation matrix
Before, carry out stirring evenly sterilization using 0.1-0.15% carbendazim solutions and cultivation matrix.
Compared with prior art, cultural method of the present invention has imitated the wild environment of suitable Herba Dendrobii growth, while choosing
Preferentially matter is efficiently fermented cultivation matrix, and the illumination of strict control plantation, temperature, humidity and Planting Row Distance etc. are required, can not only
Make cultivation product and wild product possess same quality, and significantly improve yield, survival rate and the Drying rate of Dendrobium chrysanthum, subtract
Few disease pest incidence rate, while also having easy to operate, the low remarkable advantage of cultivation cost.
Specific embodiment
The present invention is described in further detail with reference to embodiments, the present embodiment is only to make clearer to the present invention
Illustrate, rather than limitation of the present invention.It should be pointed out that for the person of ordinary skill of the art, without departing from this
On the premise of bright design, some deformations and improvement can also be made, these are within the scope of the present invention.
Embodiment 1
A kind of imitative wild tissue culture method for planting of Herba Dendrobii, comprises the following steps：
（1）Seed cultivation：Using wild Herba Dendrobii mature fruit as explant, in being seeded to seedling culture base, it is in temperature
23 DEG C, intensity of illumination 4000Lx, cultivate 65 days under conditions of light application time 12h/d, obtain Herba Dendrobii seedling；
（2）Seed and seedling disinfection：Herba Dendrobii seedling is taken, with 0.2% carbendazim solution Herba Dendrobii seedling root 10min is soaked；
（3）Build plantation embanked field：Site preparation, does growth 4.0m, width 1.0m, the embanked field of high 0.22m, and walking for wide 0.5m is stayed between embanked field
Road；
（4）It is prepared by cultivation matrix：In terms of parts by weight, by following raw materials according mixed fermentation 42d, cultivation matrix is obtained：Peat soil 22
Part, 16 parts of fertile soil, 14 parts of yellow soil, Cortex Pini ground product（Cross 30 mesh sieves after Cortex Pini is crushed to be prepared）15 parts, Camphor tree
Bark ground product（Cross 30 mesh sieves after Cortex cinnamomi camphorae is crushed to be prepared）6 parts, milled powders of cornstalk minces（30 are crossed after corn stalk is crushed
Mesh sieve is prepared）8 parts, 10 parts of Bombyx mori L., 10 parts of chicken manure；After cultivation matrix ripening, before laying cultivation matrix, use
0.15% carbendazim solution carries out stirring evenly sterilization with the cultivation matrix after ripening；
（5）Cultivation matrix is laid：One layer of rubble is first laid on embanked field, the cultivation matrix of the high 8cm in a leafing embanked field is re-layed；
（6）Seedling is planted：By the Herba Dendrobii seedling dibbling after sterilization on cultivation matrix, and root water is poured in seedling root,
Seedling line-spacing is controlled to 10cm × 10cm, and plantation is sterilized using 20% Amici after 7 days up to 1000 times of liquid of suspending agent；
（7）Plantation management：During plantation, the temperature for controlling to plant place is 22-25 DEG C, air humidity is 80-82%, shelters from heat or light
Degree 70-72%, intensity of illumination are 3500-5000Lx.
Embodiment 2
A kind of imitative wild tissue culture method for planting of Herba Dendrobii, comprises the following steps：
（1）Seed cultivation：Using wild Herba Dendrobii mature fruit as explant, in being seeded to seedling culture base, it is in temperature
25 DEG C, intensity of illumination 3000Lx, cultivate 80 days under conditions of light application time 8h/d, obtain Herba Dendrobii seedling；
（2）Seed and seedling disinfection：Herba Dendrobii seedling is taken, with 0.2% carbendazim solution Herba Dendrobii seedling root 10min is soaked；
（3）Build plantation embanked field：Site preparation, does growth 3.8m, width 1.0m, the embanked field of high 0.2m, and walking for wide 0.4m is stayed between embanked field
Road；
（4）It is prepared by cultivation matrix：In terms of parts by weight, by following raw materials according mixed fermentation 35d, cultivation matrix is obtained：Peat soil 20
Part, 20 parts of fertile soil, 15 parts of yellow soil, Cortex Pini ground product（Cross 40 mesh sieves after Cortex Pini is crushed to be prepared）10 parts, Camphor tree
Bark ground product（Cross 20 mesh sieves after Cortex cinnamomi camphorae is crushed to be prepared）10 parts, milled powders of cornstalk minces（Mistake after corn stalk is crushed
30 mesh sieves are prepared）5 parts, 10 parts of Bombyx mori L., 10 parts of chicken manure；After cultivation matrix ripening, before laying cultivation matrix, use
0.15% carbendazim solution carries out stirring evenly sterilization with the cultivation matrix after ripening；
（5）Cultivation matrix is laid：One layer of rubble is first laid on embanked field, the cultivation matrix of the high 6cm in a leafing embanked field is re-layed；
（6）Seedling is planted：By the Herba Dendrobii seedling dibbling after sterilization on cultivation matrix, and root water is poured in seedling root,
Seedling line-spacing is controlled to 10cm × 10cm, and plantation is sterilized using 15% Amici after 7 days up to 1000 times of liquid of suspending agent；
（7）Plantation management：During plantation, control plant place temperature be 26 DEG C, air humidity be 82%, shade density 74%,
Intensity of illumination is 6000Lx.
Embodiment 3
A kind of imitative wild tissue culture method for planting of Herba Dendrobii, comprises the following steps：
（1）Seed cultivation：Using wild Herba Dendrobii mature fruit as explant, in being seeded to seedling culture base, it is in temperature
26 DEG C, intensity of illumination 2500Lx, cultivate 70 days under conditions of light application time 10h/d, obtain Herba Dendrobii seedling；
（2）Seed and seedling disinfection：Herba Dendrobii seedling is taken, with 0.1% carbendazim solution Herba Dendrobii seedling root 10min is soaked；
（3）Build plantation embanked field：Site preparation, does growth 4.2m, width 1.0m, the embanked field of high 2.5m, and walking for wide 0.6m is stayed between embanked field
Road；
（4）It is prepared by cultivation matrix：In terms of parts by weight, by following raw materials according mixed fermentation 30d, cultivation matrix is obtained：Peat soil 15
Part, 20 parts of fertile soil, 12 parts of yellow soil, Cortex Pini ground product（Cross 40 mesh sieves after Cortex Pini is crushed to be prepared）15 parts, Camphor tree
Bark ground product（Cross 40 mesh sieves after Cortex cinnamomi camphorae is crushed to be prepared）8 parts, milled powders of cornstalk minces（40 are crossed after corn stalk is crushed
Mesh sieve is prepared）10 parts, 12 parts of Bombyx mori L., 8 parts of chicken manure；After cultivation matrix ripening, before laying cultivation matrix, 0.1% is used
Carbendazim solution carries out stirring evenly sterilization with the cultivation matrix after ripening；
（5）Cultivation matrix is laid：One layer of rubble is first laid on embanked field, the cultivation matrix of the high 7cm in a leafing embanked field is re-layed；
（6）Seedling is planted：By the Herba Dendrobii seedling dibbling after sterilization on cultivation matrix, and root water is poured in seedling root,
Seedling line-spacing is controlled to 10cm × 10cm, and plantation is sterilized using 20% Amici after 7 days up to 1000 times of liquid of suspending agent；
（7）Plantation management：During plantation, control plant place temperature be 26 DEG C, air humidity be 80%, shade density 70%,
Intensity of illumination is 7000Lx.
Claims (5)
1. the imitative wild tissue culture method for planting of a kind of Herba Dendrobii, it is characterised in that comprise the following steps：
（1）Seed cultivation：Using wild Herba Dendrobii mature fruit as explant, in being seeded to seedling culture base, it is in temperature
22-26 DEG C, intensity of illumination 2500-4000Lx, cultivate 60-80 days under conditions of light application time 8-12h/d, obtain Herba Dendrobii kind
Seedling；
（2）Seed and seedling disinfection：Herba Dendrobii seedling is taken, with 0.15-0.2% carbendazim solutions Herba Dendrobii seedling root 10- is soaked
12min；
（3）Build plantation embanked field：Site preparation, does growth 3.8-4.2m, width 0.9-1.1m, the embanked field of high 0.2-2.5m, between embanked field
Stay the aisle of wide 0.4-0.6m；
（4）It is prepared by cultivation matrix：In terms of parts by weight, by following raw materials according mixed fermentation 30-45d, cultivation matrix is obtained：Peat soil
15-22 parts, fertile soil 16-20 parts, yellow soil 12-16 parts, Cortex Pini ground product 10-15 parts, Cortex cinnamomi camphorae ground product 5-10 parts, jade
Rice bar ground product 5-10 parts, Bombyx mori L. 8-12 parts, chicken manure 8-12 parts；
（5）Cultivation matrix is laid：One layer of rubble is first laid on embanked field, the cultivation matrix of the high 6-9cm in a leafing embanked field is re-layed；
（6）Seedling is planted：By the Herba Dendrobii seedling dibbling after sterilization on cultivation matrix, and root water is poured in seedling root,
Seedling line-spacing is controlled to 8-12cm × 8-12cm, and plantation is killed using 15-20% Amicis after 7 days up to 1000 times of liquid of suspending agent
Bacterium；
（7）Plantation management：During plantation, the temperature for controlling to plant place is 20-28 DEG C, air humidity is 80-85%, shelters from heat or light
Degree 70-74%, intensity of illumination are 3500-7000Lx.
2. the imitative wild tissue culture method for planting of Herba Dendrobii according to claim 1, it is characterised in that step（4）In, it is described
Cortex Pini ground product be Cortex Pini is crushed after cross 20-40 mesh sieves be prepared.
3. the imitative wild tissue culture method for planting of Herba Dendrobii according to claim 1, it is characterised in that step（4）In, it is described
Cortex cinnamomi camphorae ground product be Cortex cinnamomi camphorae is crushed after cross 20-40 mesh sieves be prepared.
4. the imitative wild tissue culture method for planting of Herba Dendrobii according to claim 1, it is characterised in that step（4）In, it is described
Milled powders of cornstalk mince be corn stalk is crushed after cross 20-40 mesh sieves be prepared.
5. according to the imitative wild tissue culture method for planting of the arbitrary Herba Dendrobii of claim 1-4, it is characterised in that plant in laying
Before training substrate, carry out stirring evenly sterilization using 0.1-0.15% carbendazim solutions and cultivation matrix.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108439966A (en) * | 2018-05-11 | 2018-08-24 | 安徽霍山县草之灵中药材有限公司 | A kind of preparation method for the cultivation matrix reducing Dendrobidium huoshanness incidence |
| CN109006438A (en) * | 2018-09-07 | 2018-12-18 | 潘华章 | A kind of implantation methods of Huoshan rice dry measure used in former times |
| CN111328646A (en) * | 2020-04-08 | 2020-06-26 | 安徽霍山麟鼎轩生物科技有限公司 | Dendrobium huoshanense mother dendrobium huoshanense separate cultivation method |
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| CN108439966A (en) * | 2018-05-11 | 2018-08-24 | 安徽霍山县草之灵中药材有限公司 | A kind of preparation method for the cultivation matrix reducing Dendrobidium huoshanness incidence |
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| CN111328646A (en) * | 2020-04-08 | 2020-06-26 | 安徽霍山麟鼎轩生物科技有限公司 | Dendrobium huoshanense mother dendrobium huoshanense separate cultivation method |
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