CN106508173B - Method for promoting dragon fruit seed germination - Google Patents

Abstract

The invention discloses a method for promoting the germination of pitaya seeds. Before the germination and cultivation of the pitaya seeds, the pitaya seeds are soaked for 6-24 hours with a seed soaking agent, wherein the seed soaking agent contains 50-250 mg/L of red Mycin, kinetin at a concentration of 5-30 mg/L, and DA-6 at a concentration of 5-15 mg/L. The method of the invention improves the vigor of dragon fruit seeds, has high germination uniformity and good seedling quality.

Description

How to promote the germination of dragon fruit seeds

technical field

The present invention relates to the technical field of plant seed germination. More specifically, the present invention relates to a method for promoting the germination of dragon fruit seeds.

Background technique

Dragon fruit (Latin name: Hylocereus undulatus Britt), also known as red dragon fruit, dragon pearl fruit, fairy honey fruit, jade dragon fruit. It is a plant of the cactus family and the genus of measuring days. Dragon fruit is a tropical and subtropical fruit, originating from Costa Rica, Guatemala, Panama, Ecuador, Cuba, Colombia and other places in Central America, and later introduced to Vietnam, Thailand and other Southeast Asian countries and Taiwan Province of China, Hainan, Guangxi, Guangdong in mainland China , Fujian, Yunnan and other provinces. Dragon fruit is rich in nutrients and has unique functions. It contains vegetable albumin and anthocyanin, which are rare in ordinary plants, rich in vitamins and water-soluble dietary fiber. Dragon fruit is a cool fruit. Under natural conditions, the fruit matures in summer and autumn. Sweet and juicy. The propagation method of dragon fruit is mainly by cuttings and seed sowing. Due to the small size of dragon fruit seeds, hard seed shells, and difficulty in absorbing water, the phenomenon of slow seed germination, irregular germination and low germination rate after sowing, and dragon fruit seeds are easy to germinate. Adhesion to the pulp will easily cause mildew during germination and affect the germination effect.

SUMMARY OF THE INVENTION

An object of the present invention is to solve at least the above-mentioned problems and to provide at least the advantages which will be explained later.

Another object of the present invention is to provide a method for promoting the germination of pitaya seeds, through the seed soaking treatment, the germination rate of the pitaya seeds is improved, the germination time is shortened, and the problems of weak germination power, low germination rate and weak seedlings are solved.

In order to achieve these objects and other advantages according to the present invention, a method for promoting the germination of pitaya seeds is provided, and before the pitaya seeds are germinated and cultivated, the pitaya seeds are soaked in a seed soaking agent for 6-24h, and the seed soaking agent in the seed soaking agent Contains gibberellin at a concentration of 50-250 mg/L, kinetin at a concentration of 5-30 mg/L, and DA-6 at a concentration of 5-15 mg/L.

Preferably, in the described method for promoting the germination of dragon fruit seeds, the concentration of gibberellin in the seed soaking agent is 150 mg/L, the concentration of kinetin is 8 mg/L, the concentration of DA-6 is 10 mg/L, and the concentration of DA-6 is 10 mg/L. The soaking time of the agent is 12h.

Preferably, in the described method for promoting the germination of pitaya seeds, before the pitaya seeds are soaked in a seed soaking agent, the pitaya seeds are first disinfected, and the specific steps are as follows: placing the pretreated pitaya seeds in a The mass fraction is 0.5% bleaching powder aqueous solution for 10-30min, then immersed in 10% mass fraction trisodium phosphate aqueous solution for 10-20min, washed 3-4 times, and then irradiated with ultraviolet rays at a wavelength of 210-328nm Sterilize for 30-60min.

Preferably, the described method for promoting the germination of pitaya seeds, the method for germination and cultivation of pitaya seeds specifically refers to placing the pitaya seeds in a transparent glass petri dish with filter paper, and placing them in a constant temperature incubator for cultivation, The temperature of the constant temperature incubator is 20-32° C., the humidity of the incubator is 75-85%, and the incubation time is 10-30 days.

Preferably, in the method for promoting the germination of dragon fruit seeds, the wavelength of ultraviolet radiation disinfection treatment is 250nm, and the time is 40min.

Preferably, in the described method for promoting the germination of dragon fruit seeds, the seed soaking agent also contains nano-zinc oxide with a concentration of 3 mg/L and a Puhuang water extract with a concentration of 5 mg/L, and the solvent of the seed soaking agent It is magnetized water, and the water extract of Puhuang is that Puhuang and water are mixed according to the mass ratio of 1:25, boiled at high heat for 5 hours, and then filtered with 100-mesh gauze to obtain;

The soaking treatment of pitaya seeds by the seed soaking agent specifically includes the following steps: firstly placing the pitaya seeds soaked in the seed soaking agent together with the seed soaking agent at 4 ° C for soaking treatment for 5 hours, then placing them in a 200-300 Gauss magnetic field for magnetization treatment for 3 hours, and finally placing The soaking treatment was performed at 30° C. for 4 h, and the soaking treatments at 4° C. and 30° C. were both carried out under the irradiation of ultraviolet rays with a wavelength of 250-300 nm.

Preferably, in the described method for promoting the germination of pitaya seeds, the pretreatment specifically refers to harvesting the ripe pitaya fruit, peeled and cut into small pieces, and placed in a sieve bag with a hole diameter less than 20 meshes , rub under running water, separate the pitaya pulp and seeds, wash the separated pitaya seeds and place them in a ventilated place to dry in the shade for later use.

The present invention includes at least the following beneficial effects:

1) The method for promoting the germination of dragon fruit seeds provided by the invention, by soaking the appropriate time of the seed soaking agent containing suitable concentration, significantly promoting the germination of dragon fruit seeds, making the germination rate of dragon fruit seeds up to more than 90%, can reduce The mildew phenomenon of dragon fruit seeds significantly improves the germination rate of dragon fruit seeds, solves the problems of weak germination power, low germination rate and thin seedlings, and is simple to operate, low in cost, and easy to operate manually, sowing dragon fruit seeds in the future. Provide the basis for nursery and breeding work;

2) the present invention also carries out soaking treatment by the seed soaking agent containing suitable content of nano zinc oxide and Puhuang water extract, combined soaking and magnetic field magnetization treatment, further improves the germination rate of dragon fruit seeds, and shortens its germination time simultaneously, Among them, nano-zinc oxide has a large specific surface area, which can promote the absorption of other components by dragon fruit seeds, and because nano-zinc oxide is a photocatalyst, under ultraviolet light irradiation, it can stimulate active oxygen and kill bacteria and viruses. , and Puhuang water extract can enhance the ability of pitaya seeds to tolerate hypoxia, improve the respiration of seeds, enhance the germination potential, shorten the germination time, and promote the germination of seeds.

Other advantages, objects, and features of the present invention will appear in part from the description that follows, and in part will be appreciated by those skilled in the art from the study and practice of the invention.

Detailed ways

The present invention will be further described in detail below with reference to specific embodiments, so that those skilled in the art can implement the invention with reference to the description.

It should be noted that the experimental methods described in the following embodiments are conventional methods unless otherwise specified, and the reagents and materials can be obtained from commercial sources unless otherwise specified.

Example 1:

A method for promoting the germination of pitaya seeds, before the pitaya seeds are germinated and cultivated, the pitaya seeds are soaked for 6h with a seed soaking agent, and the seed soaking agent contains gibberellin with a concentration of 50mg/L and a concentration of 5mg/L of kinetin and DA-6 at a concentration of 5 mg/L. What is said here refers to the content of each component in each 1L of seed soaking agent, for example, the content of gibberellin per 1L of seed soaking agent is 50 mg.

DA-6 is diethylaminoethanol hexanoate.

Example 2:

A method for promoting the germination of pitaya seeds, before the pitaya seeds are germinated and cultivated, the pitaya seeds are soaked for 24h with a seed soaking agent, and the seed soaking agent contains gibberellin with a concentration of 250mg/L and a concentration of 30mg/L of kinetin and DA-6 at a concentration of 15 mg/L.

Example 3:

A method for promoting the germination of pitaya seeds, before the pitaya seeds are germinated and cultivated, the pitaya seeds are soaked for 12h with a seed soaking agent, and the seed soaking agent contains gibberellin with a concentration of 150mg/L and a concentration of 8mg/L of kinetin and DA-6 at a concentration of 10 mg/L.

Example 4:

On the basis of Example 3, the described method for promoting the germination of pitaya seeds, before the pitaya seeds are soaked with a seed soaking agent, the pitaya seeds are first sterilized, and the concrete steps are as follows: The seeds were placed in a bleaching powder aqueous solution with a mass fraction of 0.5% for 10-30min, and then placed in a 10% mass fraction of trisodium phosphate aqueous solution for 10-20min, washed 3-4 times, and then placed in an ultraviolet wavelength of 210-328nm. Under UV radiation disinfection treatment for 30-60min. The optimal conditions are that the wavelength of ultraviolet radiation disinfection treatment is set to 250nm, and the ultraviolet radiation disinfection treatment time is 40min.

The described method for promoting the germination of pitaya seeds, the method for germination and cultivation of pitaya seeds specifically refers to placing the pitaya seeds in a transparent glass petri dish with filter paper, and placing them in a constant temperature incubator for cultivation. The temperature is 20-32°C, the humidity of the incubator is 75-85%, and the incubation time is 10-30 days.

The described method for promoting the germination of dragon fruit seeds, the pretreatment specifically refers to harvesting the ripe dragon fruit fruit, peeled and cut into small pieces, placed in a mesh bag with a hole diameter less than 20 meshes, and placed under running water. Rub and wash, separate the pitaya pulp and seeds, wash the separated pitaya seeds, dry in the shade in a ventilated place, and set aside.

Example 5:

On the basis of Example 4, the described method for promoting the germination of dragon fruit seeds, the seed soaking agent also contains nano-zinc oxide with a concentration of 3mg/L and a water extract of Puhuang with a concentration of 5mg/L, the The solvent of the seed soaking agent is magnetized water, and the water extract of Puhuang is obtained by mixing Puhuang and water according to a mass ratio of 1:25, boiling with high heat for 5 hours, and filtering with 100-mesh gauze;

The soaking treatment of pitaya seeds by the seed soaking agent specifically includes the following steps: firstly placing the pitaya seeds soaked in the seed soaking agent together with the seed soaking agent at 4 ° C for soaking treatment for 5 hours, then placing them in a 200-300 Gauss magnetic field for magnetization treatment for 3 hours, and finally placing The soaking treatment was performed at 30° C. for 4 h, and the soaking treatments at 4° C. and 30° C. were both carried out under the irradiation of ultraviolet rays with a wavelength of 250-300 nm.

In order to illustrate the effect of the present invention, the inventor of the present invention is directed to Example 4, Example 5, and conventional methods (that is: after the pitaya seeds are soaked and washed with bleaching powder and trisodium phosphate aqueous solution in the conditions of Example 4, directly use The germination culture method of embodiment 4 is cultivated, specifically see embodiment 4) and the research published by people such as Hu Youyou about exogenous GA ₃ seed soaking promotes dragon fruit seed germination and draws that suitable soaking concentration is a control group (with a concentration of 400mg/L GA ₃ aqueous solution soaking treatment, soaking time is the same as Example 3, is 12h, the control group is also treated by soaking the bleaching powder and trisodium phosphate aqueous solution in the conditions of Example 4, the germination culture method is the same as the conventional method). Carry out germination treatment, according to the national standard for crop seed inspection, the germination potential, germination rate and the fresh weight of individual plant seedlings of dragon fruit seeds have been measured, the results are shown in the following table 1, wherein,

Germination potential (%)=number of germinated seeds on the 8th day/number of tested seeds×100%;

Germination rate (%) = the number of germinated seeds on the 14th day/the number of tested seeds × 100%;

Fresh weight of a single seedling = 10 plants were randomly selected to measure the fresh weight of seedlings/10.

The pitaya seed germination result contrast of the different embodiments of table 1

Approach Germination potential/% Germination rate/% Fresh weight of single seedling/mg normal method 17.8 23.2 5.9 control group 56.8 48.5 11.8 Example 4 78.6 91.8 19.6 Example 5 84.9 97.5 24.7

As can be seen from table 1, embodiment 4 compares the method for conventional method and control group with the method for embodiment 5, the pitaya seed germination rate and germination potential are obviously improved, and the fresh weight of individual plant seedlings also significantly increases, and the germination potential refers to The germination speed and germination uniformity of the seeds in the specified time indicate the strength of the seed viability, and the fresh weight of the seedlings reflects the vitality level of the seeds to a certain extent. It can be seen from this that the method of the present invention improves the pitaya seeds. The vigor, the germination uniformity is high, and the seedling quality is good.

Although the embodiment of the present invention has been disclosed as above, it is not limited to the application listed in the description and the embodiment, and it can be applied to various fields suitable for the present invention. For those skilled in the art, it can be easily Therefore, the invention is not limited to the specific details and embodiments shown and described herein without departing from the general concept defined by the appended claims and the scope of equivalents.

Claims (5)

1. A method for promoting the germination of dragon fruit seeds is characterized in that the dragon fruit seeds are soaked for 12 hours by using a seed soaking agent before the germination and culture of the dragon fruit seeds, wherein the seed soaking agent contains gibberellin with the concentration of 150mg/L, kinetin with the concentration of 8mg/L and DA-6 with the concentration of 10 mg/L;

the seed soaking agent also contains nano zinc oxide with the concentration of 3mg/L and a cattail pollen aqueous extract with the concentration of 5mg/L, the solvent of the seed soaking agent is magnetized water, the cattail pollen aqueous extract is obtained by mixing cattail pollen and water according to the mass ratio of 1:25, boiling with strong fire, decocting with slow fire for 5 hours, and filtering gauze with 100 meshes;

the soaking treatment of the dragon fruit seeds by the seed soaking agent comprises the following steps: firstly soaking the dragon fruit seeds soaked by the seed soaking agent and the seed soaking agent for 5h at 4 ℃, then placing the dragon fruit seeds in a 200-plus-300 gauss magnetic field for magnetization treatment for 3h, and finally placing the dragon fruit seeds in a 30 ℃ soaking treatment for 4h, wherein the soaking treatments at 4 ℃ and 30 ℃ are both carried out under the irradiation of ultraviolet rays with the wavelength of 250-plus-300 nm.

2. The method for promoting the germination of the dragon fruit seeds as claimed in claim 1, wherein the dragon fruit seeds are disinfected before being soaked with the seed soaking agent, and the method comprises the following specific steps: placing the pretreated dragon fruit seeds in 0.5 mass percent of bleaching powder aqueous solution for 10-30min, then placing the dragon fruit seeds in 10 mass percent of trisodium phosphate aqueous solution for soaking for 10-20min, cleaning for 3-4 times, and then carrying out ultraviolet radiation disinfection treatment for 30-60min under the condition that the ultraviolet wavelength is 210-328 nm.

3. The method for promoting the germination of the dragon fruit seeds as claimed in claim 1, wherein the method for cultivating the dragon fruit seeds by germination specifically comprises the steps of putting the dragon fruit seeds into a transparent glass culture dish with filter paper, and putting the dragon fruit seeds into a constant-temperature incubator for cultivation, wherein the temperature of the constant-temperature incubator is 20-32 ℃, the humidity of the incubator is 75-85%, and the cultivation time is 10-30 days.

4. The method for promoting germination of dragon fruit seeds as claimed in claim 2, wherein the ultraviolet radiation disinfection treatment is performed at a wavelength of 250nm for a period of 40 min.

5. The method for promoting germination of dragon fruit seeds as claimed in claim 2, wherein the pre-treatment specifically refers to collecting mature dragon fruit, peeling, cutting into small pieces, placing the small pieces in a sieve bag with a pore size smaller than 20 meshes, rubbing and washing under running water, separating dragon fruit pulp and seeds, cleaning the separated dragon fruit seeds, and then placing the cleaned dragon fruit seeds in a ventilated place for drying in the shade for later use.