CN102870525B - Method for quickly testing quality of cercis canadensis seeds - Google Patents
Method for quickly testing quality of cercis canadensis seeds Download PDFInfo
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- CN102870525B CN102870525B CN201210357063.3A CN201210357063A CN102870525B CN 102870525 B CN102870525 B CN 102870525B CN 201210357063 A CN201210357063 A CN 201210357063A CN 102870525 B CN102870525 B CN 102870525B
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- 238000000034 method Methods 0.000 title claims abstract description 26
- 238000012360 testing method Methods 0.000 title claims abstract description 20
- 240000000024 Cercis siliquastrum Species 0.000 title abstract description 5
- 235000000131 Cercis siliquastrum Nutrition 0.000 title abstract description 4
- 230000035784 germination Effects 0.000 claims abstract description 13
- 239000003448 gibberellin Substances 0.000 claims abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 9
- 229920000742 Cotton Polymers 0.000 claims abstract description 8
- 238000002791 soaking Methods 0.000 claims abstract description 8
- 241000246150 Cercis Species 0.000 claims description 28
- 235000006228 Cercis occidentalis Nutrition 0.000 claims description 28
- 238000005213 imbibition Methods 0.000 claims description 15
- 238000005286 illumination Methods 0.000 claims description 6
- 239000004576 sand Substances 0.000 claims description 4
- 230000003203 everyday effect Effects 0.000 claims description 3
- 239000011159 matrix material Substances 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 238000011218 seed culture Methods 0.000 claims description 2
- 230000005059 dormancy Effects 0.000 abstract description 13
- 229930191978 Gibberellin Natural products 0.000 abstract description 8
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 abstract description 7
- 238000005520 cutting process Methods 0.000 abstract description 3
- 238000005516 engineering process Methods 0.000 abstract description 2
- 238000012258 culturing Methods 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 6
- 238000007689 inspection Methods 0.000 description 5
- 238000012545 processing Methods 0.000 description 5
- 230000007226 seed germination Effects 0.000 description 4
- 238000009738 saturating Methods 0.000 description 3
- 238000010899 nucleation Methods 0.000 description 2
- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical compound C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 description 1
- 241000220487 Bauhinia Species 0.000 description 1
- 235000018893 Cercis canadensis var canadensis Nutrition 0.000 description 1
- 241001573881 Corolla Species 0.000 description 1
- 240000008375 Hymenaea courbaril Species 0.000 description 1
- 240000007472 Leucaena leucocephala Species 0.000 description 1
- 235000010643 Leucaena leucocephala Nutrition 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 244000061458 Solanum melongena Species 0.000 description 1
- 235000018907 Tylosema fassoglense Nutrition 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 210000003484 anatomy Anatomy 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- MYSWGUAQZAJSOK-UHFFFAOYSA-N ciprofloxacin Chemical compound C12=CC(N3CCNCC3)=C(F)C=C2C(=O)C(C(=O)O)=CN1C1CC1 MYSWGUAQZAJSOK-UHFFFAOYSA-N 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 208000001848 dysentery Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- IXORZMNAPKEEDV-OBDJNFEBSA-N gibberellin A3 Chemical class C([C@@]1(O)C(=C)C[C@@]2(C1)[C@H]1C(O)=O)C[C@H]2[C@]2(C=C[C@@H]3O)[C@H]1[C@]3(C)C(=O)O2 IXORZMNAPKEEDV-OBDJNFEBSA-N 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000007781 pre-processing Methods 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000014284 seed dormancy process Effects 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Landscapes
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a method for quickly testing quality of cercis canadensis seeds. The method comprises the following steps of: firstly, cutting seed coats open; secondly, placing the seeds in a germination bed which is laid with absorbent cotton to absorb water slowly until the seeds are sufficiently imbibed; thirdly, soaking the sufficiently imbibed seeds in a 450-550mg.L<-1> gibberellin solution for 20 to 24 hours; and finally placing the seed in an illuminating incubator at the temperature of 25 DEG C, culturing for 14 days, and checking germination of the seed. The method can be operated simply and quickly, ensures that the seeds are released from dormancy and germinate in a short time, is a highly efficient and reliable technology for testing the quality of the cercis canadensis seed before planting and seedling raising, has a broad application prospect and high practical application value, and can produce good economic and social benefits.
Description
Technical field
The present invention relates to seed testing method, be specifically related to the method for the Canadian cercis seed quality of a kind of quick test.
Background technology
Canada cercis (Cercis canadensis L.), Caesalpiniaceae, Redbud, originates in eastern united states, distribution is from the New Jersey of northern US and western Pennsylvania to south, the state of Michigan and the southeast, the Nebraska State, and Texas and Florida State are arrived in south.Canada's cercis blade heart, Newborn Leaves is pale red purple, gradually transfers bottle green to later, has gloss.Before fall foliage, that leaf look is is golden yellow, Exocarpium Citri Rubrum, aubergine, with distinct contrast, extremely beautiful.Canada cercis first leaf in the early spring is bloomed, and corolla is flat, circle, and reddish violet, grows thickly or is raceme, and flowers are in great numbers brilliant, are famous ornamental plantss.Spending of Canada cercis is edible, is also one of important plant producing nectar.Density of wood is large, hard, and striped is fine and closely woven.The astringent extracting from bark can be used for treating dysentery.
Canada cercis is that China recent years is extensively introduced a fine variety the Landscape Trees of planting, and both can plant in garden, park, also can plant in roadside.In production, Canadian cercis is main still breeds with seed, but this seed has obvious dormancy habit, and this has brought a lot of inconvenience to seed testing, field sowing work.Research in the past shows, the main cause of whole beans order class plant dormancy is because seed has fine and close seed coat or pericarp, thereby the absorption that has hindered moisture has suppressed seed germination, as the seed of the plants such as the locust tree of Papilionaceae, Mimosaceae Acacia, be strong property seed.This class seed, as long as can take some measure to make seed water suction, the sprouting that seed can be neat fast.The bauhinia that belongs to Caesalpiniaceae with Canadian cercis together is also like this.But the seed of Canadian cercis, except the physics dormancy producing because of the saturating property of seed coat, endosperm also has obvious inhibitory action to the sprouting of seed.Therefore in the time removing the dormancy of Canadian cercis seed except taking corresponding measure to increase the saturating property of seed coat, also need to remove the physiological dormancy that endosperm produces.So for the seed dormant reason of Canadian cercis, develop easy, to remove efficiently its dormancy method the quality inspection tool that accelerates this seed be of great significance.
Summary of the invention
Goal of the invention: for the seed dormant reason of Canadian cercis, the object of this invention is to provide the method for the Canadian cercis seed quality of a kind of quick test simply, efficiently, carry out smoothly effective ways are provided for seed trade ground.
Technical scheme: in order to realize foregoing invention object, the technical solution used in the present invention is:
The method of the Canadian cercis seed quality of a kind of quick test: first cut seed coat (can the cotyledon Bottoming of seed be realized with single-edge blade), again seed is placed on the germinating bed that is covered with absorbent cotton and slowly absorbs water to abundant imbibition, by the seed 450-550mgL of fully imbibition
-1gibberellins solution seed soaking 20-24h, then seed is cultivated to 14d in 25 DEG C of illumination boxs, check germination.
Described slow water suction, the time is 30-36h.
Described Gibberellins solution concentration is 500mgL
-1, seed soaking time is 24h.
Between described culture period, give the illumination of 8h daytime every day.
Described seed culture, germination matrix is absorbent cotton or the river sand through sterilization.
The method is excised cotyledon end slightly, can make the fully imbibition of all seeds, and can not make seed in preprocessing process because treatment conditions are improper and dead.Although period of the day from 11 p.m. to 1 a.m end is slightly injured when pretreatment in addition, this can not affect sprouting, the growth of seed after breaking dormancy.The Imbibing of seed preferably adopts seed is placed in to slowly water suction on absorbent cotton.This preprocess method be particularly suitable as growing seedlings of the quick quality inspection of indoor seed and a small amount of seed.The method is to make percentage of seedgermination reach 83%.
Beneficial effect: compared with prior art, the method for the Canadian cercis seed quality of quick test of the present invention, employing is used gibberellin seed soaking after cutting seed coat, Seed imbibition again, can make percentage of seedgermination reach 83%; Not fully imbibition of treated seeds, seed can not be sprouted; Seed after imbibition does not adopt gibberellin processing, and germination rate also only has 10%; Therefore, seed testing method of the present invention, simple to operate, quick, can complete seed dormant releasing within a short period of time and make its sprouting, for the quality inspection of seed before Canadian cercis seeding and seedling raising provides the technological means of high efficient and reliable, have a extensive future, there is good actual application value, can produce good economic benefit and social benefit.
Brief description of the drawings
Fig. 1 is the internal anatomy of Canadian cercis seed.
Embodiment
Below in conjunction with specific embodiment, the present invention is described further.
Example 1
In seed testing, for Canadian cercis seed can be sprouted smoothly, need to first remove physics dormancy and the physiological dormancy of seed.Remove the physics dormancy of seed, remove the impact of fine and close seed coat on seed water suction, therefore, every method that can make the saturating property of seed coat be improved can be selected in principle.But in actual use, still there is variety of problems.The present embodiment, with the method for excision cotyledon end, to how removing Canadian cercis seed dormancy, and carries out seed testing, makes detailed explanation.
The method of the Canadian cercis seed quality of a kind of quick test, use again gibberellin seed-soaking method after seed coat (excision cotyledon end), Seed imbibition for cutting: the seed coat of seed is cut with single-edge blade, because seed coat is hard, can adopt in cotyledon Bottoming sub-fraction and realize incision seed coat, as shown in Figure 1, again seed is placed on the germinating bed that is covered with absorbent cotton and slowly absorbs water to the abundant imbibition of seed, approximately need 30-36h, by the seed 500mgL of fully imbibition
-1gibberellins solution seed soaking, then seed is placed in 25 DEG C of illumination boxs and cultivates 14d, between culture period, give the illumination of 8h daytime every day, checks germination.Specific experiment result is as shown in table 1.
Table 1 excises the germination rate of processing again seed after cotyledon end with gibberellin
As known from Table 1, this method of inspection, first excises cotyledon end, and its physics dormancy is removed in fully imbibition of seed, and in the time that Canadian cercis seed only adopts this processing method, after imbibition, the germination rate of seed only has 15%.The seed of imbibition is used gibberellin processing again, is mainly the physiological dormancy of removing endosperm, from table 1, finds, germination rate obviously improves, and illustrates that gibberellin processing is conducive to promote the sprouting of Canadian cercis seed.Because the method for excision cotyledon end can not make seed death, therefore this processing is specially adapted to the method as indoor Canadian cercis seed testing.
Embodiment 2
With reference to the method for embodiment 1, by further Optimal Experimental, by the abundant seed of imbibition, use 450-550mgL
-1gibberellins solution, seed soaking 20-24h, germination rate is the highest, test effect is best.Do germinating bed with absorbent cotton, need often to change germinating bed, antipollution germinating bed exerts an influence to seed germination.Germination matrix is the river sand through sterilization, notes making river sand to keep moisture state during seed sprouting.Repeatedly change after germinating bed, germinating bed does not pollute, and seed can be sprouted smoothly, as shown in Figure 2.
Therefore, the method, can be used for the quick test of seed quality, simple to operate, quick, can complete seed dormant releasing within a short period of time and it is sprouted, for the quality inspection of seed before Canadian cercis seeding and seedling raising provides the technological means of high efficient and reliable, have a extensive future.
Claims (3)
1. a method for quick test Canada cercis seed quality, is characterized in that: first cut seed coat, then seed is placed on the germinating bed that is covered with absorbent cotton and slowly absorbs water to abundant imbibition, by the seed 500mgL of fully imbibition
-1gibberellins solution seed soaking 24h, then seed is cultivated to 14d in 25 DEG C of illumination boxs, check germination; Described slow water suction, the time is 30-36h.
2. the method for the Canadian cercis seed quality of quick test according to claim 1, is characterized in that: between described culture period, give the illumination of 8h daytime every day.
3. the method for the Canadian cercis seed quality of quick test according to claim 1, is characterized in that: described seed culture, germination matrix is absorbent cotton or the river sand through sterilization.
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| CN201210357063.3A CN102870525B (en) | 2012-09-24 | 2012-09-24 | Method for quickly testing quality of cercis canadensis seeds |
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| CN201210357063.3A CN102870525B (en) | 2012-09-24 | 2012-09-24 | Method for quickly testing quality of cercis canadensis seeds |
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| CN102870525A CN102870525A (en) | 2013-01-16 |
| CN102870525B true CN102870525B (en) | 2014-10-29 |
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| CN106879275B (en) * | 2017-01-10 | 2020-11-17 | 中国科学院华南植物园 | Method for rapidly and effectively improving germination rate of sophora tonkinensis seeds |
| CN108633379B (en) * | 2018-06-14 | 2020-12-11 | 长江大学 | Method for breaking dormancy of cercis gigantea seeds |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20090118286A (en) * | 2008-05-13 | 2009-11-18 | 한국과학기술원 | Method for modifying the germination of plant seed by controlling the level of somnus or functional equivalents thereof in cell |
| CN102308689A (en) * | 2011-06-27 | 2012-01-11 | 苏芹 | Technique for accelerating germination of flower seeds |
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2012
- 2012-09-24 CN CN201210357063.3A patent/CN102870525B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20090118286A (en) * | 2008-05-13 | 2009-11-18 | 한국과학기술원 | Method for modifying the germination of plant seed by controlling the level of somnus or functional equivalents thereof in cell |
| CN102308689A (en) * | 2011-06-27 | 2012-01-11 | 苏芹 | Technique for accelerating germination of flower seeds |
Non-Patent Citations (6)
| Title |
|---|
| Ertekin, M.Improving germination of Judas tree (Cercis siliquastrum) seeds by hormone treatment.《Seed Science and Technology》.2010,第38卷(第3期), |
| Improving germination of Judas tree (Cercis siliquastrum) seeds by hormone treatment;Ertekin, M;《Seed Science and Technology》;20101031;第38卷(第3期);595-601 * |
| Robert L. Geneve.Seed Dormancy in Eastern Redbud (Cercis canadensis).《Journal of the American Society for Horticultural Science》.1991,第116卷(第1期), |
| Rodney O. Jones，等.Seedcoat Structure Related to Germination in Eastern Redbud (Cercis canadensis L.).《Journal of the American Society for Horticultural Science》.1995,第120卷(第1期), * |
| Seed Dormancy in Eastern Redbud (Cercis canadensis);Robert L. Geneve;《Journal of the American Society for Horticultural Science》;19911231;第116卷(第1期);第85页右栏第2段，第87页左栏和表3 * |
| 孙秀琴,等.紫荆种子休眠解除及促进萌发的研究.《林业科学研究》.1998,第11卷(第4期), * |
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Effective date of registration: 20201230 Address after: 225000 Lidian town science and Technology Pioneer Park, Guangling District, Yangzhou City, Jiangsu Province Patentee after: Yangzhou Shengning Information Technology Co.,Ltd. Address before: Nanjing City, Jiangsu province 210037 Longpan Road No. 159 Patentee before: NANJING FORESTRY University |
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