CN102870525A - Method for quickly testing quality of cercis canadensis seeds - Google Patents
Method for quickly testing quality of cercis canadensis seeds Download PDFInfo
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- CN102870525A CN102870525A CN2012103570633A CN201210357063A CN102870525A CN 102870525 A CN102870525 A CN 102870525A CN 2012103570633 A CN2012103570633 A CN 2012103570633A CN 201210357063 A CN201210357063 A CN 201210357063A CN 102870525 A CN102870525 A CN 102870525A
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- seed
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- cercis
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- germination
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- 238000000034 method Methods 0.000 title claims abstract description 30
- 238000012360 testing method Methods 0.000 title claims abstract description 22
- 240000000024 Cercis siliquastrum Species 0.000 title abstract 3
- 235000000131 Cercis siliquastrum Nutrition 0.000 title abstract 3
- 230000035784 germination Effects 0.000 claims abstract description 13
- 239000003448 gibberellin Substances 0.000 claims abstract description 13
- 229930191978 Gibberellin Natural products 0.000 claims abstract description 9
- 238000002791 soaking Methods 0.000 claims abstract description 9
- 229920000742 Cotton Polymers 0.000 claims abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 4
- 241000246150 Cercis Species 0.000 claims description 32
- 235000006228 Cercis occidentalis Nutrition 0.000 claims description 31
- 238000005213 imbibition Methods 0.000 claims description 15
- 238000005286 illumination Methods 0.000 claims description 6
- 239000004576 sand Substances 0.000 claims description 4
- 230000003203 everyday effect Effects 0.000 claims description 3
- 239000011159 matrix material Substances 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- IXORZMNAPKEEDV-OBDJNFEBSA-N gibberellin A3 Chemical class C([C@@]1(O)C(=C)C[C@@]2(C1)[C@H]1C(O)=O)C[C@H]2[C@]2(C=C[C@@H]3O)[C@H]1[C@]3(C)C(=O)O2 IXORZMNAPKEEDV-OBDJNFEBSA-N 0.000 claims description 2
- 238000011218 seed culture Methods 0.000 claims description 2
- 238000004659 sterilization and disinfection Methods 0.000 claims description 2
- 230000005059 dormancy Effects 0.000 abstract description 13
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 abstract description 7
- 238000005520 cutting process Methods 0.000 abstract description 4
- 238000005516 engineering process Methods 0.000 abstract description 2
- 238000012258 culturing Methods 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 8
- 230000007226 seed germination Effects 0.000 description 7
- 238000007689 inspection Methods 0.000 description 5
- 238000009738 saturating Methods 0.000 description 3
- 238000010899 nucleation Methods 0.000 description 2
- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical group C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 description 1
- 241000220487 Bauhinia Species 0.000 description 1
- 235000018893 Cercis canadensis var canadensis Nutrition 0.000 description 1
- 241000675108 Citrus tangerina Species 0.000 description 1
- 241001573881 Corolla Species 0.000 description 1
- 240000008375 Hymenaea courbaril Species 0.000 description 1
- 240000007472 Leucaena leucocephala Species 0.000 description 1
- 235000010643 Leucaena leucocephala Nutrition 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 244000061458 Solanum melongena Species 0.000 description 1
- 235000018907 Tylosema fassoglense Nutrition 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 210000003484 anatomy Anatomy 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 208000001848 dysentery Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000007781 pre-processing Methods 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 230000014284 seed dormancy process Effects 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
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Abstract
The invention discloses a method for quickly testing quality of cercis canadensis seeds. The method comprises the following steps of: firstly, cutting seed coats open; secondly, placing the seeds in a germination bed which is laid with absorbent cotton to absorb water slowly until the seeds are sufficiently imbibed; thirdly, soaking the sufficiently imbibed seeds in a 450-550mg.L<-1> gibberellin solution for 20 to 24 hours; and finally placing the seed in an illuminating incubator at the temperature of 25 DEG C, culturing for 14 days, and checking germination of the seed. The method can be operated simply and quickly, ensures that the seeds are released from dormancy and germinate in a short time, is a highly efficient and reliable technology for testing the quality of the cercis canadensis seed before planting and seedling raising, has a broad application prospect and high practical application value, and can produce good economic and social benefits.
Description
Technical field
The present invention relates to the seed testing method, be specifically related to the method for the Canadian cercis seed quality of a kind of quick test.
Background technology
The Canada cercis (
Cercis canadensisL.), Caesalpiniaceae, Redbud originates in the eastern united states, and distribution is from the New Jersey of northern US and western Pennsylvania to south, the state of Michigan and the southeast, the Nebraska State, and Texas and Florida State are arrived in south.Canada's cercis blade is heart-shaped, and Newborn Leaves is pale red purple, gradually transfers later on bottle green to, has gloss.Before the fall foliage, the leaf look is golden yellow, tangerine, aubergine, and is with distinct contrast, unusually beautiful.Canada elder generation in cercis early spring leaf is bloomed, and corolla is flat, circle, and reddish violet is grown thickly or is raceme, and flowers are in great numbers brilliant, are famous ornamental plantss.Spending of Canada cercis is edible, also is one of important plant that produces nectar.Density of wood is large, and is hard, and striped is fine and closely woven.The astringent that extracts from bark can be used for treating dysentery.
The Canada cercis is that China recent years is extensively introduced a fine variety the Landscape Trees of planting, and both can plant in garden, park, also can plant in the roadside.Canadian cercis mainly still breeds with seed in the production, but this seed has obvious dormancy habit, and this has brought a lot of inconvenience for seed testing, field sowing work.Studies show that in the past, the main cause of whole beans order class plant dormancy is because seed has fine and close kind skin or pericarp, thereby the absorption that has hindered moisture has suppressed seed germination, and the seed such as plants such as the locust tree of Papilionaceae, Mimosaceae Acacias is strong property seed.This class seed, as long as can take some measure to make seed suction, the sprouting that seed is can be fast neat.The bauhinia that belongs to Caesalpiniaceae with Canadian cercis together also is like this.But the seed of Canadian cercis, except the physics dormancy that produces because plant the saturating property of skin, endosperm also has obvious inhibitory action to Seed germination.Therefore when removing the dormancy of Canadian cercis seed except taking corresponding measure to increase the saturating property of kind of skin, also need remove the physiological dormancy that endosperm produces.So for the seed dormant reason of Canadian cercis, develop method easy, that remove its dormancy efficiently the quality inspection tool that accelerates this seed be of great significance.
Summary of the invention
Goal of the invention: for the seed dormant reason of Canadian cercis, the purpose of this invention is to provide the method for the Canadian cercis seed quality of a kind of simply, efficiently quick test, carrying out smoothly for seed trade ground provides effective ways.
Technical scheme: in order to realize the foregoing invention purpose, the technical solution used in the present invention is:
The method of the Canadian cercis seed quality of a kind of quick test: cut first kind of a skin (can the cotyledon Bottoming of seed be realized with single-edge blade), again seed is placed slowly to absorb water to abundant imbibition on the germinating bed that is covered with absorbent cotton, with the seed 450-550mgL of fully imbibition
-1Gibberellins solution seed soaking 20-24h, then seed is cultivated 14d in 25 ℃ of illumination boxs, check germination.
Described slow suction, the time is 30-36h.
Described Gibberellins solution concentration is 500mgL
-1, seed soaking time is 24h.
Between described culture period, give the illumination of 8h daytime every day.
Described seed culture, germination matrix are absorbent cotton or the river sand through sterilizing.
The method is excised the cotyledon end slightly, can make the fully imbibition of all seeds, and can not make seed in preprocessing process because treatment conditions are improper and dead.Although period of the day from 11 p.m. to 1 a.m end is slightly injured during preliminary treatment in addition, this can not affect Seed germination behind the breaking dormancy, growth.The Imbibing of seed preferably adopts seed is placed slowly suction on the absorbent cotton.This preprocess method be particularly suitable as growing seedlings of the quick quality inspection of indoor seed and a small amount of seed.The method is to make percentage of seedgermination reach 83%.
Beneficial effect: compared with prior art, the method for the Canadian cercis seed quality of quick test of the present invention, employing is used the gibberellin seed soaking after cutting kind of a skin, Seed imbibition again, can make percentage of seedgermination reach 83%; Not fully imbibition of treated seeds, seed can not be sprouted; Seed after the imbibition does not adopt gibberellin to process, and germination rate also only has 10%; Therefore, seed testing method of the present invention, simple to operate, quick, but finish seed dormant releasing within a short period of time and make its sprouting, the technological means of high efficient and reliable is provided for the quality inspection of seed before the Canadian cercis seeding and seedling raising, have a extensive future, have good actual application value, can produce preferably economic benefit and social benefit.
Description of drawings
Fig. 1 is the internal anatomy of Canadian cercis seed;
Fig. 2 is that seed can be sprouted as a result figure smoothly.
Embodiment
The present invention is described further below in conjunction with specific embodiment.
Example 1
In seed testing, can sprout smoothly in order to make Canadian cercis seed, need to remove first physics dormancy and the physiological dormancy of seed.Remove the physics dormancy of seed, namely remove the fine and close skin of planting to the impact of seed suction, therefore, every method that the saturating property of kind of skin is improved can be selected in principle.But in the use of reality, still there is variety of problems.Present embodiment is with the method for excision cotyledon end, to how removing Canadian cercis seed dormancy, and carries out seed testing, makes detailed explanation.
The method of the Canadian cercis seed quality of a kind of quick test, after cutting kind of a skin (the excision cotyledon is terminal), Seed imbibition, use again the gibberellin seed-soaking method: cut with the kind skin of single-edge blade with seed, because it is hard to plant skin, can adopt in cotyledon Bottoming sub-fraction and realize cutting kind of a skin, as shown in Figure 1, again seed is placed and slowly absorb water to the abundant imbibition of seed on the germinating bed that is covered with absorbent cotton, need approximately 30-36h, with the seed 500mgL of fully imbibition
-1Gibberellins solution seed soaking, then seed is placed in 25 ℃ of illumination boxs and cultivates 14d, give the illumination of 8h daytime every day between culture period, checks germination.Concrete experimental result is as shown in table 1.
Process again the germination rate of seed behind the table 1 excision cotyledon end with gibberellin
As known from Table 1, this method of inspection is excised first the cotyledon end, and its physics dormancy is removed in fully imbibition of seed, and when Canadian cercis seed only adopted this processing method, the germination rate of seed only had 15% after the imbibition.The seed of imbibition is processed with gibberellin again, mainly is the physiological dormancy of removing endosperm, finds that from table 1 germination rate obviously improves, and illustrates that the gibberellin processing is conducive to promote Canadian cercis Seed germination.Because the method for excision cotyledon end can not make seed dead, so this processing is specially adapted to the method as indoor Canadian cercis seed testing.
Embodiment 2
With reference to the method for embodiment 1, by further Optimal Experimental, with the abundant seed of imbibition, use 450-550mgL
-1Gibberellins solution, the seed soaking 20-24h, germination rate is the highest, test effect is best.Do germinating bed with absorbent cotton, need often to change germinating bed, antipollution germinating bed exerts an influence to seed germination.Germination matrix is the river sand through sterilization, notes making river sand to keep moisture state during the seed sprouting.After repeatedly changing germinating bed, germinating bed does not pollute, and seed can be sprouted smoothly, as shown in Figure 2.
Therefore, the method can be used for the quick test of seed quality, simple to operate, quick, but finish seed dormant releasing within a short period of time and it is sprouted, for the quality inspection of seed before the Canadian cercis seeding and seedling raising provides the technological means of high efficient and reliable, have a extensive future.
Claims (5)
1. the method for a quick test Canada cercis seed quality is characterized in that: cut first kind of a skin, seed is placed slowly to absorb water to abundant imbibition on the germinating bed that is covered with absorbent cotton again, with the seed 450-550mgL of fully imbibition
-1Gibberellins solution seed soaking 20-24h, then seed is cultivated 14d in 25 ℃ of illumination boxs, check germination.
2. the method for quick test according to claim 1 Canada cercis seed quality, it is characterized in that: described slow suction, the time is 30-36h.
3. the method for quick test according to claim 1 Canada cercis seed quality, it is characterized in that: described Gibberellins solution concentration is 500mgL
-1, seed soaking time is 24h.
4. the method for the Canadian cercis seed quality of quick test according to claim 1 is characterized in that: between described culture period, give the illumination of 8h daytime every day.
5. the method for quick test according to claim 1 Canada cercis seed quality is characterized in that: described seed culture, germination matrix are absorbent cotton or through the river sand of sterilization.
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| CN201210357063.3A CN102870525B (en) | 2012-09-24 | 2012-09-24 | Method for quickly testing quality of cercis canadensis seeds |
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| CN201210357063.3A CN102870525B (en) | 2012-09-24 | 2012-09-24 | Method for quickly testing quality of cercis canadensis seeds |
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| CN102870525A true CN102870525A (en) | 2013-01-16 |
| CN102870525B CN102870525B (en) | 2014-10-29 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106879275A (en) * | 2017-01-10 | 2017-06-23 | 中国科学院华南植物园 | A kind of quick method for effectively improving Hainan Chinese scholartree percentage of seedgermination |
| CN108633379A (en) * | 2018-06-14 | 2018-10-12 | 长江大学 | A method of abolishing huge cercis seed dormancy |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20090118286A (en) * | 2008-05-13 | 2009-11-18 | 한국과학기술원 | Method for modifying the germination of plant seed by controlling the level of somnus or functional equivalents thereof in cell |
| CN102308689A (en) * | 2011-06-27 | 2012-01-11 | 苏芹 | Technique for accelerating germination of flower seeds |
-
2012
- 2012-09-24 CN CN201210357063.3A patent/CN102870525B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20090118286A (en) * | 2008-05-13 | 2009-11-18 | 한국과학기술원 | Method for modifying the germination of plant seed by controlling the level of somnus or functional equivalents thereof in cell |
| CN102308689A (en) * | 2011-06-27 | 2012-01-11 | 苏芹 | Technique for accelerating germination of flower seeds |
Non-Patent Citations (4)
| Title |
|---|
| ERTEKIN, M: "Improving germination of Judas tree (Cercis siliquastrum) seeds by hormone treatment", 《SEED SCIENCE AND TECHNOLOGY》 * |
| ROBERT L. GENEVE: "Seed Dormancy in Eastern Redbud (Cercis canadensis)", 《JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE》 * |
| RODNEY O. JONES，等: "Seedcoat Structure Related to Germination in Eastern Redbud (Cercis canadensis L.)", 《JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE》 * |
| 孙秀琴，等: "紫荆种子休眠解除及促进萌发的研究", 《林业科学研究》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106879275A (en) * | 2017-01-10 | 2017-06-23 | 中国科学院华南植物园 | A kind of quick method for effectively improving Hainan Chinese scholartree percentage of seedgermination |
| CN108633379A (en) * | 2018-06-14 | 2018-10-12 | 长江大学 | A method of abolishing huge cercis seed dormancy |
| CN108633379B (en) * | 2018-06-14 | 2020-12-11 | 长江大学 | Method for breaking dormancy of cercis gigantea seeds |
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| CN102870525B (en) | 2014-10-29 |
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Application publication date: 20130116 Assignee: Shanghai Yao Qi Garden Technology Co., Ltd. Assignor: Nanjing Forestry University Contract record no.: 2018320000223 Denomination of invention: Method for quickly testing quality of cercis canadensis seeds Granted publication date: 20141029 License type: Common License Record date: 20181022 |
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Application publication date: 20130116 Assignee: Beijing Huamei Wanxiang Technology Co., Ltd. Assignor: Nanjing Forestry University Contract record no.: 2018320000235 Denomination of invention: Method for quickly testing quality of cercis canadensis seeds Granted publication date: 20141029 License type: Common License Record date: 20181024 |
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Effective date of registration: 20201230 Address after: 225000 Lidian town science and Technology Pioneer Park, Guangling District, Yangzhou City, Jiangsu Province Patentee after: Yangzhou Shengning Information Technology Co.,Ltd. Address before: Nanjing City, Jiangsu province 210037 Longpan Road No. 159 Patentee before: NANJING FORESTRY University |
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Effective date of registration: 20210121 Address after: Longpan road Xuanwu District of Nanjing city of Jiangsu Province, No. 159 210037 Patentee after: NANJING FORESTRY University Address before: 225000 Lidian town science and Technology Pioneer Park, Guangling District, Yangzhou City, Jiangsu Province Patentee before: Yangzhou Shengning Information Technology Co.,Ltd. |
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Effective date of registration: 20210225 Address after: 225000 Lidian town science and Technology Pioneer Park, Guangling District, Yangzhou City, Jiangsu Province Patentee after: Yangzhou Shengning Information Technology Co.,Ltd. Address before: Longpan road Xuanwu District of Nanjing city of Jiangsu Province, No. 159 210037 Patentee before: NANJING FORESTRY University |
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