CN106719439A - A kind of leech hatching method - Google Patents
A kind of leech hatching method Download PDFInfo
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- CN106719439A CN106719439A CN201611053807.7A CN201611053807A CN106719439A CN 106719439 A CN106719439 A CN 106719439A CN 201611053807 A CN201611053807 A CN 201611053807A CN 106719439 A CN106719439 A CN 106719439A
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- 230000012447 hatching Effects 0.000 title claims abstract description 86
- 241000545744 Hirudinea Species 0.000 title claims abstract description 31
- 238000000034 method Methods 0.000 title claims abstract description 25
- 239000002689 soil Substances 0.000 claims abstract description 36
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 31
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 31
- 210000004681 ovum Anatomy 0.000 claims abstract description 31
- 238000011534 incubation Methods 0.000 claims abstract description 27
- 241001247197 Cephalocarida Species 0.000 claims abstract description 23
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims abstract description 12
- 229910002092 carbon dioxide Inorganic materials 0.000 claims abstract description 6
- 239000001569 carbon dioxide Substances 0.000 claims abstract description 6
- 244000189799 Asimina triloba Species 0.000 claims description 21
- 235000006264 Asimina triloba Nutrition 0.000 claims description 21
- 235000009467 Carica papaya Nutrition 0.000 claims description 21
- 240000002853 Nelumbo nucifera Species 0.000 claims description 21
- 235000006508 Nelumbo nucifera Nutrition 0.000 claims description 21
- 235000006510 Nelumbo pentapetala Nutrition 0.000 claims description 21
- 239000000706 filtrate Substances 0.000 claims description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 241000037831 Polygonatum sibiricum Species 0.000 claims description 11
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 11
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 11
- 229910052661 anorthite Inorganic materials 0.000 claims description 11
- GWWPLLOVYSCJIO-UHFFFAOYSA-N dialuminum;calcium;disilicate Chemical compound [Al+3].[Al+3].[Ca+2].[O-][Si]([O-])([O-])[O-].[O-][Si]([O-])([O-])[O-] GWWPLLOVYSCJIO-UHFFFAOYSA-N 0.000 claims description 11
- 150000007523 nucleic acids Chemical class 0.000 claims description 11
- 102000039446 nucleic acids Human genes 0.000 claims description 11
- 108020004707 nucleic acids Proteins 0.000 claims description 11
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 10
- 239000012141 concentrate Substances 0.000 claims description 10
- 235000019441 ethanol Nutrition 0.000 claims description 10
- 244000146486 Glehnia littoralis Species 0.000 claims description 8
- 235000004036 Glehnia littoralis Nutrition 0.000 claims description 8
- 238000002360 preparation method Methods 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 6
- 108010016626 Dipeptides Proteins 0.000 claims description 5
- 235000009508 confectionery Nutrition 0.000 claims description 5
- 125000005909 ethyl alcohol group Chemical group 0.000 claims description 5
- 238000000605 extraction Methods 0.000 claims description 5
- 238000010438 heat treatment Methods 0.000 claims description 5
- 238000005286 illumination Methods 0.000 claims description 5
- 238000010992 reflux Methods 0.000 claims description 5
- 239000007921 spray Substances 0.000 claims description 5
- 241000258623 Whitmania pigra Species 0.000 abstract description 5
- 230000004083 survival effect Effects 0.000 abstract description 4
- 230000036039 immunity Effects 0.000 abstract description 3
- 238000012360 testing method Methods 0.000 description 9
- 230000000052 comparative effect Effects 0.000 description 8
- 244000132059 Carica parviflora Species 0.000 description 6
- 235000014653 Carica parviflora Nutrition 0.000 description 6
- 229920001661 Chitosan Polymers 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 239000011806 microball Substances 0.000 description 5
- 241000238582 Artemia Species 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 4
- 239000004480 active ingredient Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 206010001928 Amenorrhoea Diseases 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 208000026106 cerebrovascular disease Diseases 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 238000007726 management method Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000008736 traumatic injury Effects 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Fodder In General (AREA)
Abstract
The present invention discloses a kind of leech hatching method, comprises the following steps：（1）The ovum cocoon of collection is washed away into soil, then foraminate one end is placed in incubation plate upward, often disk puts 120 140 ovum cocoons；（2）It is uniform in ovum cocoon to be covered with 1 2cm thickness hatch soils；（3）On hatch soil artemia hatching solution was sprayed every 3 days；（4）Hatch the 1st 8 day, it is 23 25 DEG C, the 9th 17 day to control hatching room temperature, control hatching room temperature, it is 68 75% that the relative air humidity of hatching house is controlled during hatching, and the concentration for controlling to hatch indoor carbon dioxide is 480 550ppm, daily using the 60min of violet exposure incubation plate 40.The method provided using the present invention, can cause that Whitmania pigra Whitman spawns, with incubation rate higher and concentration degree of emerging, can shorten emerging the cycle for leech, and hatch the leech seedling for coming have stronger immunity, improve the survival rate of leech seedling.
Description
Technical field
The invention belongs to hirudiniculture field, and in particular to a kind of leech hatching method.
Background technology
Leech, popular name leech,《Sheng Nong's herbal classic》In have been described, with medical value very high；In inland fresh water
Growth and breeding in waters, is the extraordinary medicinal aquatic animal of Chinese tradition, and the brewed rear traditional Chinese medical science of its dried product is used as medicine, with treatment
The effects such as wind, hypertension, the clear stasis of blood, amenorrhoea, traumatic injury.In recent years new discovery leech preparation is in preventing and treating cardiovascular and cerebrovascular disease and anticancer
Aspect has special efficacy.In history based on fishing for naturally, because agricultural chemicals, chemical fertilizer etc. are abused in recent years, and industrial or agricultural " three wastes " is right for it
The pollution of environment, wild natural resources falls sharply, and with the deep development of leech medical value, its market demand potential is huge.Though
Under right leech natural environment can spawning and hatching, obtain seedling, but the influence of the polytropy and natural enemy due to weather, cause leech
Incubation rate it is relatively low, only 15% or so, and it is poor to hatch the leech constitution come, it is easy to dead, significantly limit leech
The development of aquaculture, therefore it is badly in need of a kind of scientific and reasonable artificial leech hatching method.
The content of the invention
In order to solve the above problems, the present invention provides a kind of leech hatching method.
The present invention is achieved by the following technical solutions.
A kind of leech hatching method, comprises the following steps：
（1）The ovum cocoon of collection is washed away into soil, then foraminate one end is placed in incubation plate upward, often disk puts 120-
140 ovum cocoons；
（2）Uniform in ovum cocoon to be covered with 1-2cm thickness hatch soils, wherein hatch soil is made up of the component of following weight portion：Shell gathers
Sugared microballoon 80-90 parts, aetite 20-30 parts, diatomite 40-50 parts, anorthite 10-15 parts；
（3）On hatch soil every 3 days spray artemia hatching solution, each incubation plate sprinkling 200-300g artemia hatching solutions, wherein artemia hatching solution by
The component of following weight portion is made：1-3 parts of yeast nucleic acid NA100, Ganguertai 9-13 parts, pawpaw 20-22 parts, lotus seeds 16-19
Part, radix polygonati officinalis 16-20 parts, glehnia littoralis 23-25 parts, polygonatum sibiricum Redoute 21-24 parts, fructus alpiniae oxyphyllae 15-17 parts, water 180-200 parts；
（4）Hatch the 1-8 days, it is 23-25 DEG C to control hatching room temperature, and the 9-17 days, it was 26-27 DEG C to control hatching room temperature,
It is 28-30 DEG C to control hatching room temperature afterwards, and the relative air humidity of hatching house is controlled during hatching for 68-75%, control hatching
The concentration of indoor carbon dioxide is 480-550ppm, and violet exposure incubation plate 40-60min, the intensity of illumination of purple light are used daily
It is 120-140 luxs.
Specifically, above-mentioned steps（2）In hatch soil be made of following methods：Aetite, diatomite, anorthite are mixed
After conjunction, 60-80 mesh is crushed to, is then added thereto to chitosan microball, stirred, hatch soil is obtained.
Specifically, above-mentioned steps（3）In the preparation method of artemia hatching solution comprise the following steps：
（a）Radix polygonati officinalis, glehnia littoralis, polygonatum sibiricum Redoute, fructus alpiniae oxyphyllae are put into water, after big fire boils 2-3 hours, are filtered to get filtrate, will
It is standby that filtrate is down to room temperature；
（b）Lotus seeds and pawpaw are mixed, is ground, be put into 1.4 times of absolute ethyl alcohols of weight of its weight, heating and refluxing extraction 2-3 is small
When obtain extract solution, extract solution is filtered, the filtered solution of gained reclaims ethanol and is simultaneously concentrated into relative density for 1.2g/cm3, concentrate
Temperature be 55 DEG C, when concentrate is down to room temperature, obtain pawpaw lotus seed extract solution；
（c）Pawpaw lotus seed extract solution is added to step（a）In gained filtrate, then it is added thereto to yeast nucleic acid NA100, sweet
Paddy dipeptides, stirs, and artemia hatching solution is obtained.
Technical scheme more than, the beneficial effects of the invention are as follows：
The method provided using the present invention, can cause that Whitmania pigra Whitman spawns have incubation rate higher and concentration degree of emerging, and can shorten water
Leech emerges the cycle, and hatches the leech seedling for coming and have stronger immunity, improves the survival rate of leech seedling.Its
In, after chitosan microball and diatomite mixing, adhesion of the Whitmania pigra Whitman spawns to hatch soil can be improved, and material after its mixing
The Surface Modification Effect can improve the activity of ovum cocoon cell, improve the incubation rate of ovum cocoon；Active ingredient in aetite and anorthite
After synergy, the permeability of the film on ovum cocoon surface can be improved, increase the respiration of ovum cocoon cell, accelerate speed of emergence；Incubate
Change yeast nucleic acid NA100 and the Ganguertai synergy in liquid, can greatly improve the activity of ovum cocoon cell, lifting ovum cocoon
Incubation rate simultaneously accelerates speed of emergence；The various nutriments required containing ovum cocoon hatching in pawpaw and lotus seeds, are able to continuously
Acted synergistically for ovum cocoon hatching provides active ingredient in nutrition, and radix polygonati officinalis and glehnia littoralis, ovum cocoon can be increased to nutrient
Absorbability, further increases the incubation rate of ovum cocoon；After active ingredient synergy in polygonatum sibiricum Redoute and fructus alpiniae oxyphyllae, can pole
Big lifting hatches the immunity of the leech seedling for coming, and reduces the death rate of leech nursery；In hatching process, strict control
The humiture and gas concentration lwevel of hatching house, can for ovum cocoon hatching provide stabilization environment, and these environmental factors and
Violet exposure acts synergistically, and can further activate the activity of ovum cocoon cell, improves incubation rate and hatching speed.
Specific embodiment
Following examples are used to illustrate the present invention, but can not be used for limiting the scope of the present invention.The reality used in embodiment
The condition of applying can be for further adjustments according to the condition of producer, and unaccounted implementation condition is usually conventional laboratory conditions.
Embodiment 1
A kind of leech hatching method, comprises the following steps：
（1）The ovum cocoon of collection is washed away into soil, then foraminate one end is placed in incubation plate upward, often disk puts 120
Ovum cocoon；
（2）Uniform in ovum cocoon to be covered with 1cm thickness hatch soils, wherein hatch soil is made up of the component of following weight portion：Shitosan
80 parts of microballoon, 20 parts of aetite, 40 parts of diatomite, 10 parts of anorthite；
（3）Artemia hatching solution was sprayed every 3 days on hatch soil, each incubation plate sprays 200g artemia hatching solutions, and wherein artemia hatching solution is by following
The component of weight portion is made：1 part of yeast nucleic acid NA100,9 parts of Ganguertai, 20 parts of pawpaw, 16 parts of lotus seeds, 16 parts of radix polygonati officinalis, coral
23 parts of coral dish, 21 parts of polygonatum sibiricum Redoute, 15 parts of fructus alpiniae oxyphyllae, 180 parts of water；
（4）Hatch the 1-8 days, it is 23 DEG C to control hatching room temperature, and the 9-17 days, it was 26 DEG C to control hatching room temperature, is controlled afterwards
System hatching room temperature is 28 DEG C, and it is 68% that the relative air humidity of hatching house is controlled during hatching, control hatching indoor carbon dioxide
Concentration be 480ppm, violet exposure incubation plate 40min is used daily, the intensity of illumination of purple light is 120 luxs.
Specifically, above-mentioned steps（2）In hatch soil be made of following methods：Aetite, diatomite, anorthite are mixed
After conjunction, 60 mesh are crushed to, are then added thereto to chitosan microball, stirred, hatch soil is obtained.
Specifically, above-mentioned steps（3）In the preparation method of artemia hatching solution comprise the following steps：
（a）Radix polygonati officinalis, glehnia littoralis, polygonatum sibiricum Redoute, fructus alpiniae oxyphyllae are put into water, after big fire boils 2 hours, are filtered to get filtrate, will filtered
It is standby that liquid is down to room temperature；
（b）Lotus seeds and pawpaw are mixed, is ground, be put into 1.4 times of absolute ethyl alcohols of weight of its weight, heating and refluxing extraction 2 hours
Extract solution is obtained, extract solution is filtered, the filtered solution of gained reclaims ethanol and is simultaneously concentrated into relative density for 1.2g/cm3, concentrate
Temperature is 55 DEG C, when concentrate is down to room temperature, obtains pawpaw lotus seed extract solution；
（c）Pawpaw lotus seed extract solution is added to step（a）In gained filtrate, then it is added thereto to yeast nucleic acid NA100, sweet
Paddy dipeptides, stirs, and artemia hatching solution is obtained.
Comparative example 1
In comparative example 1, step（2）Hatch soil be soil after common sterilization, remaining step is identical with embodiment 1.
Embodiment 2
A kind of leech hatching method, comprises the following steps：
（1）The ovum cocoon of collection is washed away into soil, then foraminate one end is placed in incubation plate upward, often disk puts 130
Ovum cocoon；
（2）Uniform in ovum cocoon to be covered with 1cm thickness hatch soils, wherein hatch soil is made up of the component of following weight portion：Shitosan
85 parts of microballoon, 25 parts of aetite, 45 parts of diatomite, 13 parts of anorthite；
（3）Artemia hatching solution was sprayed every 3 days on hatch soil, each incubation plate sprays 250g artemia hatching solutions, and wherein artemia hatching solution is by following
The component of weight portion is made：2 parts of yeast nucleic acid NA100,11 parts of Ganguertai, 21 parts of pawpaw, 18 parts of lotus seeds, 18 parts of radix polygonati officinalis, coral
24 parts of coral dish, 23 parts of polygonatum sibiricum Redoute, 16 parts of fructus alpiniae oxyphyllae, 190 parts of water；
（4）Hatch the 1-8 days, it is 24 DEG C to control hatching room temperature, and the 9-17 days, it was 26 DEG C to control hatching room temperature, is controlled afterwards
System hatching room temperature is 29 DEG C, and it is 72% that the relative air humidity of hatching house is controlled during hatching, control hatching indoor carbon dioxide
Concentration be 520ppm, violet exposure incubation plate 50min is used daily, the intensity of illumination of purple light is 130 luxs.
Specifically, above-mentioned steps（2）In hatch soil be made of following methods：Aetite, diatomite, anorthite are mixed
After conjunction, 70 mesh are crushed to, are then added thereto to chitosan microball, stirred, hatch soil is obtained.
Specifically, above-mentioned steps（3）In the preparation method of artemia hatching solution comprise the following steps：
（a）Radix polygonati officinalis, glehnia littoralis, polygonatum sibiricum Redoute, fructus alpiniae oxyphyllae are put into water, after big fire boils 2.5 hours, are filtered to get filtrate, will
It is standby that filtrate is down to room temperature；
（b）Lotus seeds and pawpaw are mixed, is ground, be put into 1.4 times of absolute ethyl alcohols of weight of its weight, heating and refluxing extraction 2.5 is small
When obtain extract solution, extract solution is filtered, the filtered solution of gained reclaims ethanol and is simultaneously concentrated into relative density for 1.2g/cm3, concentrate
Temperature be 55 DEG C, when concentrate is down to room temperature, obtain pawpaw lotus seed extract solution；
（c）Pawpaw lotus seed extract solution is added to step（a）In gained filtrate, then it is added thereto to yeast nucleic acid NA100, sweet
Paddy dipeptides, stirs, and artemia hatching solution is obtained.
Comparative example 2
In comparative example 2, step（3）Artemia hatching solution be distilled water after sterilization, remaining step is identical with embodiment 2.
Embodiment 3
A kind of leech hatching method, comprises the following steps：
（1）The ovum cocoon of collection is washed away into soil, then foraminate one end is placed in incubation plate upward, often disk puts 120-
140 ovum cocoons；
（2）Uniform in ovum cocoon to be covered with 2cm thickness hatch soils, wherein hatch soil is made up of the component of following weight portion：Shitosan
90 parts of microballoon, 30 parts of aetite, 50 parts of diatomite, 15 parts of anorthite；
（3）Artemia hatching solution was sprayed every 3 days on hatch soil, each incubation plate sprays 300g artemia hatching solutions, and wherein artemia hatching solution is by following
The component of weight portion is made：3 parts of yeast nucleic acid NA100,13 parts of Ganguertai, 22 parts of pawpaw, 19 parts of lotus seeds, 20 parts of radix polygonati officinalis, coral
25 parts of coral dish, 24 parts of polygonatum sibiricum Redoute, 17 parts of fructus alpiniae oxyphyllae, 200 parts of water；
（4）Hatch the 1-8 days, it is 25 DEG C to control hatching room temperature, and the 9-17 days, it was 27 DEG C to control hatching room temperature, is controlled afterwards
System hatching room temperature is 30 DEG C, and it is 75% that the relative air humidity of hatching house is controlled during hatching, control hatching indoor carbon dioxide
Concentration be 550ppm, violet exposure incubation plate 60min is used daily, the intensity of illumination of purple light is 140 luxs.
Specifically, above-mentioned steps（2）In hatch soil be made of following methods：Aetite, diatomite, anorthite are mixed
After conjunction, 80 mesh are crushed to, are then added thereto to chitosan microball, stirred, hatch soil is obtained.
Specifically, above-mentioned steps（3）In the preparation method of artemia hatching solution comprise the following steps：
（a）Radix polygonati officinalis, glehnia littoralis, polygonatum sibiricum Redoute, fructus alpiniae oxyphyllae are put into water, after big fire boils 3 hours, are filtered to get filtrate, will filtered
It is standby that liquid is down to room temperature；
（b）Lotus seeds and pawpaw are mixed, is ground, be put into 1.4 times of absolute ethyl alcohols of weight of its weight, heating and refluxing extraction 3 hours
Extract solution is obtained, extract solution is filtered, the filtered solution of gained reclaims ethanol and is simultaneously concentrated into relative density for 1.2g/cm3, concentrate
Temperature is 55 DEG C, when concentrate is down to room temperature, obtains pawpaw lotus seed extract solution；
（c）Pawpaw lotus seed extract solution is added to step（a）In gained filtrate, then it is added thereto to yeast nucleic acid NA100, sweet
Paddy dipeptides, stirs, and artemia hatching solution is obtained.
Comparative example 3
In comparative example 3, humiture, gas concentration lwevel to hatching house are not controlled, and do not use violet exposure, remaining
Step is identical with embodiment 3.
Experiment：
Hatch the ovum cocoon given birth to a collection of close leech, totally 6 ten thousand, stochastic averagina point with the method in each embodiment and comparative example respectively
It it is six groups, test group 1,3,5 is hatched using the method for embodiment 1,2,3, the method that test group 2,5,6 uses comparative example 1,2,3
Hatching, remaining hatching management method uses conventional technology, and result of the test is as shown in table 1：
The hatching Whitmania pigra Whitman spawns situation of table 1
| Project | Incubation rate/% | Emerge cycles/day | Survival rate/% of the leech seedling after 5 days |
| Test group 1 | 96.1 | 19 | 96.2 |
| Test group 2 | 70.2 | 24 | 82.4 |
| Test group 3 | 95.7 | 18 | 94.5 |
| Test group 4 | 68.9 | 22 | 84.0 |
| Test group 5 | 97.1 | 19 | 93.8 |
| Test group 6 | 82.5 | 21 | 86.6 |
As shown in Table 1, the method that the present invention is provided, can cause Whitmania pigra Whitman spawns with incubation rate higher, and incubation cycle compared with
Short, the survival rate of seedling is higher after hatching, greatly improves the economic benefit and cultivation enthusiasm of raiser.
It should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention and it is unrestricted.Although with reference to compared with
Good embodiment has been described in detail to the present invention, it will be understood by those within the art that, can be to the technology of invention
Scheme is modified or equivalent, and without deviating from the scope of technical solution of the present invention, it all should cover in power of the invention
In sharp claimed range.
Claims (3)
1. a kind of leech hatching method, it is characterised in that comprise the following steps：
（1）The ovum cocoon of collection is washed away into soil, then foraminate one end is placed in incubation plate upward, often disk puts 120-
140 ovum cocoons；
（2）Uniform in ovum cocoon to be covered with 1-2cm thickness hatch soils, wherein hatch soil is made up of the component of following weight portion：Shell gathers
Sugared microballoon 80-90 parts, aetite 20-30 parts, diatomite 40-50 parts, anorthite 10-15 parts；
（3）On hatch soil every 3 days spray artemia hatching solution, each incubation plate sprinkling 200-300g artemia hatching solutions, wherein artemia hatching solution by
The component of following weight portion is made：1-3 parts of yeast nucleic acid NA100, Ganguertai 9-13 parts, pawpaw 20-22 parts, lotus seeds 16-19
Part, radix polygonati officinalis 16-20 parts, glehnia littoralis 23-25 parts, polygonatum sibiricum Redoute 21-24 parts, fructus alpiniae oxyphyllae 15-17 parts, water 180-200 parts；
（4）Hatch the 1-8 days, it is 23-25 DEG C to control hatching room temperature, and the 9-17 days, it was 26-27 DEG C to control hatching room temperature,
It is 28-30 DEG C to control hatching room temperature afterwards, and the relative air humidity of hatching house is controlled during hatching for 68-75%, control hatching
The concentration of indoor carbon dioxide is 480-550ppm, and violet exposure incubation plate 40-60min, the intensity of illumination of purple light are used daily
It is 120-140 luxs.
2. a kind of leech hatching method according to claim 1, it is characterised in that step（2）In hatch soil use
Following methods are made：After aetite, diatomite, anorthite are mixed, 60-80 mesh is crushed to, is then added thereto to shitosan
Microballoon, stirs, and hatch soil is obtained.
3. a kind of leech hatching method according to claim 1, it is characterised in that step（3）In artemia hatching solution system
Preparation Method is comprised the following steps：
（a）Radix polygonati officinalis, glehnia littoralis, polygonatum sibiricum Redoute, fructus alpiniae oxyphyllae are put into water, after big fire boils 2-3 hours, are filtered to get filtrate, will
It is standby that filtrate is down to room temperature；
（b）Lotus seeds and pawpaw are mixed, is ground, be put into 1.4 times of absolute ethyl alcohols of weight of its weight, heating and refluxing extraction 2-3 is small
When obtain extract solution, extract solution is filtered, the filtered solution of gained reclaims ethanol and is simultaneously concentrated into relative density for 1.2g/cm3, concentrate
Temperature be 55 DEG C, when concentrate is down to room temperature, obtain pawpaw lotus seed extract solution；
（c）Pawpaw lotus seed extract solution is added to step（a）In gained filtrate, then it is added thereto to yeast nucleic acid NA100, sweet
Paddy dipeptides, stirs, and artemia hatching solution is obtained.
Priority Applications (1)
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| CN201611053807.7A CN106719439A (en) | 2016-11-25 | 2016-11-25 | A kind of leech hatching method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201611053807.7A CN106719439A (en) | 2016-11-25 | 2016-11-25 | A kind of leech hatching method |
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| CN106719439A true CN106719439A (en) | 2017-05-31 |
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| CN201611053807.7A Pending CN106719439A (en) | 2016-11-25 | 2016-11-25 | A kind of leech hatching method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114223591A (en) * | 2021-12-14 | 2022-03-25 | 浙江省淡水水产研究所 | Method suitable for large-scale family construction of red swamp crayfish and matched culture facility |
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