CN106491961A - A kind of Chinese medicine composition of preventing and treating Rana grylia bark rot and preparation method thereof - Google Patents
A kind of Chinese medicine composition of preventing and treating Rana grylia bark rot and preparation method thereof Download PDFInfo
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
- A61K36/076—Poria
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/288—Taraxacum (dandelion)
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/42—Cucurbitaceae (Cucumber family)
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- A61K36/18—Magnoliophyta (angiosperms)
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A61K36/18—Magnoliophyta (angiosperms)
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- A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
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- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
- A61K9/1605—Excipients; Inactive ingredients
- A61K9/1629—Organic macromolecular compounds
- A61K9/1652—Polysaccharides, e.g. alginate, cellulose derivatives; Cyclodextrin
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- A61K2236/30—Extraction of the material
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- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A61K2236/50—Methods involving additional extraction steps
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Abstract
The present invention provides a kind of Chinese medicine composition of preventing and treating Rana grylia bark rot, and in terms of parts by weight, its raw material is made up of following composition：10 70 parts of Abelmoschus esculentus, 10 80 parts of Semen Trigonellae, 2 10 parts of Radix Ophiopogonis, 5 10 parts of Herba Salviae Chinensiss, 2 10 parts of Rhizoma Polygonati Odorati, 2 10 parts of Radix Trichosanthis, 2 10 parts of Herba Taraxaci, 2 10 parts of Herba Anoectochili roxburghii, 2 10 parts of Fructus Phyllanthi, 2 10 parts of Poria, 2 10 parts of the Radix Astragali, 2 10 parts of Fructus Mori, in the medicine there is no incompatibility in each material combination, can substantially reduce the incidence rate of Rana grylia bark rot, have good preventive and therapeutic action to Rana grylia bark rot, and have no toxic side effect, using safety, remain without hazardous drugs.
Description
Technical field
The invention belongs to Rana nigromaculata cultural technique field, and in particular to a kind of Chinese medicine composition of preventing and treating Rana grylia disease and
Its preparation method.
Background technology
Rana grylia also known as the river frog, Hylarana guentheri.The U.S. is originated in, the later stage eighties 20th century introduces China, its fine and tender taste,
Delicious flavour, is first-class Rana esculenta, has promoted cultivation throughout the country.Rana grylia has that individual big, growth is fast, taste
Delicious, protein content is high, is a kind of high-quality and efficient economic cultivation batrachia.With industry competition fierceness, some old raisers
Cultural technique old stuff, causes to produce effect lowly, and some can't regain one's original capital, and some cultivation are not stinted using some forbidden drugs, cause Rana nigromaculata
Harmful substance accumulation, so as to cause mortality, also makes cultivation water suffer severe contamination in vivo, also ecological around collateral damage
Environment.
In breeding process, large-scale cultivation is susceptible to disease to Rana grylia, and is very easy to spread, mainly
There is following disease：Tadpole period Common Diseases：Disease, septicemia, enteritis, fish moldss, ascites, trichodinasis, gas bubble disease；Common into the frog
Disease：Enteritis, red leg disease, infectious liver disease, white-eyes-sickness, bark rot etc., by putting into practice, exploring, Experimental comparison, take to prevent
Based on.
Wherein Rana grylia bark rot is mainly Deficiency disease, feeds be deficient in vitamin A, D and vitamin B group for a long time
Feedstuff easily causes the disease, and the disease mostly occurred in the froglet stage；Because skin injury causes bacterium infection that this disease can also occur.Disease
Shape：The skin at some positions such as back, head, extremity etc. of the their early stage disease frog tarnishes, and occurs spending stricture of vagina in vain, is called light
Change variation, epidermal area comes off after 2～3 days, exposes white skin corium and starts to decay, Endothelial denudation exposes redness after 5～6 days
Subdermal muscle, the sick frog are not eaten motionless until dead.Prevention and controls：The feedstuff for feeding is diversified, and overall balance is wanted in nutrition, it is to avoid
Long-time feeds the single feed of not comprehensive nutrition.Add vitamin A, D and vitamin B group in feedstuff, or add fish oil and
The material rich in these vitamin such as fresh animal liver, adds " frog must health ", " strong gram 99 ", " strong gram 103 " etc. in feedstuff
Medicine feeds.
Rana grylia disease is prevented and treated using Western medicine typically in prior art, drug dependence is easily caused, or is affected Rana nigromaculata
Meat, it is therefore necessary to develop a kind of Chinese medicine composition of preventing and treating Rana grylia bark rot.
Content of the invention
An object of the present invention is to overcome the above-mentioned not enough of prior art and provide a kind of preventing and treating Rana grylia bark rot
The Chinese medicine composition of disease, in the medicine there is no incompatibility in each material combination, can substantially reduce sending out for Rana grylia bark rot
Raw rate, has good preventive and therapeutic action to Rana grylia bark rot, and has no toxic side effect, using safety, without harmful medicine
Thing is remained.
The second object of the present invention is the preparation method of the Chinese medicine composition for providing above-mentioned preventing and treating Rana grylia bark rot.
The Chinese medicine composition of present invention preventing and treating Rana grylia bark rot, in terms of parts by weight, its raw material is by following composition system
Into：
Abelmoschus esculentus 10-70 parts, Semen Trigonellae 10-80 parts, Radix Ophiopogonis 2-10 parts, Herba Salviae Chinensiss 5-10 parts, Rhizoma Polygonati Odorati 2-10 parts, Radix Trichosanthis 2-10
Part, Herba Taraxaci 2-10 parts, Herba Anoectochili roxburghii 2-10 parts, Fructus Phyllanthi 2-10 parts, Poria 2-10 parts, Radix Astragali 2-10 parts, Fructus Mori 2-10 parts.
Wherein preferred 30-50 parts of Abelmoschus esculentus, the preferred 30-45 parts of Semen Trigonellae.
Wherein preferred 4-7 parts Radix Ophiopogonis, the preferred 6-9 parts of Herba Salviae Chinensiss.
Wherein preferred 5-8 parts of Rhizoma Polygonati Odorati, the preferred 4-8 parts of Radix Trichosanthis.
Wherein preferred 4-8 parts of Herba Taraxaci, the preferred 5-9 parts of Herba Anoectochili roxburghii.
Wherein preferred 4-6 parts of Fructus Phyllanthi, the preferred 6-9 parts of Poria.
Wherein preferred 5-7 parts of the Radix Astragali, the preferred 4-8 parts of Fructus Mori.
From Abelmoschus esculentus fruit, other compositions select the prepared slices of Chinese crude drugs to wherein Abelmoschus esculentus.
The preparation method of the Chinese medicine composition of present invention preventing and treating Rana grylia bark rot, in turn includes the following steps：
(1) by Abelmoschus esculentus fruit clean after at a temperature of 0-10 DEG C cold preservation 10-14 hours, by cold preservation after Abelmoschus esculentus fruit be cut into thin
Piece, carries out completing, dries；
(2) after other prepared slices of Chinese crude drugs remove impurity such as the Abelmoschus esculentus for making and Semen Trigonellae, cleaning, the fine powder that is ground into more than 60 mesh
Shape, being placed in 80-100 times of weight boiling water carries out the cooking of 60-70 minutes, and cooled and filtered obtains filtrate I, and melt cinder adds 2-3 times of weight
90-95% ethanol solution, extract 60-80 minutes at 40-45 DEG C, vacuum distillation obtains filtrate II, and the two mixing obtains final product extraction
Liquid；Leachate is spray-dried to make dry extract, adds adjuvant, is first pressed into thick piece in advance, is then comminuted into granule；
(3) by the granule for making pack sterilizing.
Wherein step（2）The adjuvant preferably microcrystalline cellulose, Microcrystalline Cellulose with the weight ratio of dry extract is（10-
14）：1.
The medicine using method of the present invention：Into the frog phase, when feeding every time, every 10 kg diet weighs 400 grams of preventing and treating U.S.
The Chinese medicine composition of Rana nigromaculata bark rot, is subsequently adding in feedstuff, mixes, feeds immediately, and each nursing once, is freely adopted sooner or later daily
Food, 10 days is a course for the treatment of, can feed one course for the treatment of of this medicine every 10 days, and froglet phase medicine halves.
Specific embodiment
Embodiment of the present invention is described in detail below in conjunction with embodiment, but those skilled in the art will
Understand, the following example is merely to illustrate the present invention, and should not be taken as limiting the scope of the invention.
Table 1 for embodiment 1-7 formula, unit for gram
Table 1
| Embodiment | Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Embodiment 5 | Embodiment 6 |
| Abelmoschus esculentus | 15 | 70 | 45 | 40 | 50 | 30 |
| Semen Trigonellae | 70 | 15 | 40 | 45 | 35 | 45 |
| Radix Ophiopogonis | 3 | 9 | 5 | 7 | 4 | 6 |
| Herba Salviae Chinensiss | 9 | 3 | 7 | 5 | 8 | 8 |
| Rhizoma Polygonati Odorati | 3 | 9 | 5 | 7 | 5 | 6 |
| Radix Trichosanthis | 9 | 3 | 7 | 5 | 7 | 8 |
| Herba Taraxaci | 3 | 9 | 5 | 7 | 4 | 6 |
| Herba Anoectochili roxburghii | 9 | 3 | 7 | 5 | 8 | 8 |
| Fructus Phyllanthi | 3 | 9 | 5 | 7 | 4 | 6 |
| Poria | 9 | 3 | 7 | 5 | 8 | 8 |
| The Radix Astragali | 3 | 9 | 7 | 5 | 6 | 6 |
| Fructus Mori | 9 | 3 | 5 | 7 | 6 | 8 |
Table 2 for embodiment 8-14 formula, unit for gram
Table 2
| Embodiment | Embodiment 7 | 8 | 9 | 10 | 11 | 12 |
| Abelmoschus esculentus | 50 | 60 | 25 | 60 | 25 | 30 |
| Semen Trigonellae | 30 | 25 | 60 | 25 | 60 | 45 |
| Radix Ophiopogonis | 6 | 4 | 6 | 4 | 8 | 6 |
| Herba Salviae Chinensiss | 6 | 8 | 6 | 8 | 4 | 8 |
| Rhizoma Polygonati Odorati | 6 | 5 | 7 | 4 | 8 | 6 |
| Radix Trichosanthis | 6 | 7 | 5 | 8 | 4 | 8 |
| Herba Taraxaci | 6 | 4 | 6 | 4 | 8 | 6 |
| Herba Anoectochili roxburghii | 6 | 8 | 6 | 8 | 4 | 8 |
| Fructus Phyllanthi | 6 | 6 | 7 | 8 | 4 | 6 |
| Poria | 6 | 6 | 5 | 4 | 8 | 8 |
| The Radix Astragali | 6 | 8 | 5 | 4 | 8 | 6 |
| Fructus Mori | 6 | 4 | 7 | 8 | 4 | 8 |
Comparative example 1
Herba Salviae Chinensiss in embodiment 1 are removed, other formula are completely the same with embodiment 1；
Comparative example 2
Herba Anoectochili roxburghii in embodiment 1 is removed, other formula are completely the same with embodiment 1；
The preparation method of above example and comparative example health tea is as follows：
(1) by Abelmoschus esculentus fruit clean after at a temperature of 0-10 DEG C cold preservation 10-14 hours, by cold preservation after Abelmoschus esculentus fruit be cut into thin
Piece, carries out completing, dries；
(2) after other prepared slices of Chinese crude drugs remove impurity such as the Abelmoschus esculentus for making and Semen Trigonellae, cleaning, the fine powder that is ground into more than 60 mesh
Shape, being placed in 80-100 times of weight boiling water carries out the cooking of 60-70 minutes, and cooled and filtered obtains filtrate I, and melt cinder adds 2-3 times of weight
90-95% ethanol solution, extract 60-80 minutes at 40-45 DEG C, vacuum distillation obtains filtrate II, and the two mixing obtains final product Semen Maydiss
Must lixiviating solution；Leachate is spray-dried to make dry extract, adds microcrystalline cellulose excipients (14：1), thick piece is pressed in advance first,
It is then comminuted into granule；
(3) by the granule for making pack sterilizing.
Clinical trial one：
(1) laboratory animal
Select 300 Rana grylias with bark rot into the frog, loss of appetite, be reluctant activity.
(2) experimental technique
Random packet, is divided into 15 groups, and 20 per group, wherein 14 groups is experimental group, respectively feeding embodiment 1-12, comparative example
The feedstuff that medicine described in 1-2 is made into, daily each 2 times, free choice feeding sooner or later, continuous 10 days；Another group is matched group, using existing
Have the technology frog must health therapy, according still further to normal feed mode feed.Treatment is observed and is made a record for 10 days afterwards.It is specifically shown in
Table 2.
(3) treatment standard and observational technique
Cure：Bark rot is fully recovered；
Effectively：Bark rot is effectively alleviated, and has very big alleviation；
Invalid：Bark rot is not effectively alleviated.
Table 2
| Cure number | Significant figure | Invalid number | |
| Embodiment 1 | 20 | 0 | 0 |
| Embodiment 2 | 10 | 0 | 1 |
| Embodiment 3 | 10 | 1 | 0 |
| Embodiment 4 | 19 | 1 | 0 |
| Embodiment 5 | 20 | 0 | 0 |
| Embodiment 6 | 20 | 0 | 0 |
| Embodiment 7 | 19 | 1 | 0 |
| Embodiment 8 | 18 | 1 | 1 |
| Embodiment 9 | 20 | 0 | 0 |
| Embodiment 10 | 19 | 1 | 0 |
| Embodiment 11 | 18 | 1 | 1 |
| Embodiment 12 | 17 | 1 | 2 |
| Comparative example 1 | 12 | 4 | 4 |
| Comparative example 2 | 11 | 5 | 4 |
| Matched group | 8 | 4 | 8 |
It follows that in the case of equal consumption, it is right that the therapeutic effect that is mixed into using medicine of the present invention in feedstuff is substantially better than
Ratio and matched group.And after 1 month, experimental group is 0 recurrence, and matched group has 5 recurrences.
Clinical trial two：
In order to prove feeding effect of the present invention to Rana grylia, feeding experiment is repeatedly tried, choose large-scale Rana grylia and support
The Rana grylia for being in into the frog phase of field 3000 is grown, body weight size, health status are basically identical, are randomly divided into 15 groups, per group 200
Only.Wherein 14 groups is experimental group, the feedstuff that feeding embodiment 1-12, medicine described in comparative example 1-2 are made into respectively, sooner or later daily
Respectively twice, free choice feeding, continuous 10 days；One course for the treatment of of this medicine was fed every 10 days.Another group is matched group, feeds not dosing
The identical feedstuff of thing, the nursing number of times of 15 groups of Rana nigromaculatas, feeding volume, nursing time all same.Contrast the various performances of 15 groups of Rana nigromaculatas
Index, meansigma methodss such as table 3.
After 1 month, 15 groups of Rana nigromaculata situation such as tables 3：
Table 3
| Ill evil number | Moon weightening (g)/only | |
| Embodiment 1 | 0 | 112 |
| Embodiment 2 | 7 | 99 |
| Embodiment 3 | 0 | 109 |
| Embodiment 4 | 0 | 104 |
| Embodiment 5 | 0 | 107 |
| Embodiment 6 | 0 | 111 |
| Embodiment 7 | 2 | 107 |
| Embodiment 8 | 0 | 100 |
| Embodiment 9 | 4 | 102 |
| Embodiment 10 | 0 | 105 |
| Embodiment 11 | 1 | 106 |
| Embodiment 12 | 0 | 108 |
| Comparative example 1 | 23 | 85 |
| Comparative example 2 | 30 | 81 |
| Matched group | 51 | 72 |
Can be evident that from table, after the medicine that the continuous feeding present invention is provided, daily gain is more right for experimental group Rana nigromaculata
Increase substantially according to group, and experimental group is compared with matched group honey stomach, energetic, state is good, occur without disease substantially.
Claims (9)
1. a kind of preventing and treating Rana grylia bark rot Chinese medicine composition, it is characterised in that in terms of parts by weight, its raw material is by as follows
Composition is made：Abelmoschus esculentus 10-70 parts, Semen Trigonellae 10-80 parts, Radix Ophiopogonis 2-10 parts, Herba Salviae Chinensiss 5-10 parts, Rhizoma Polygonati Odorati 2-10 parts, Radix Trichosanthis
2-10 parts, Herba Taraxaci 2-10 parts, Herba Anoectochili roxburghii 2-10 parts, Fructus Phyllanthi 2-10 parts, Poria 2-10 parts, Radix Astragali 2-10 parts, Fructus Mori 2-
10 parts.
2. according to claim 1 preventing and treating Rana grylia bark rot Chinese medicine composition, it is characterised in that described for 30-50
Part, Semen Trigonellae is 30-45 parts.
3. according to claim 1 preventing and treating Rana grylia bark rot Chinese medicine composition, it is characterised in that the wherein wheat
Winter is 4-7 parts, and Herba Salviae Chinensiss are 6-9 parts.
4. according to claim 1 preventing and treating Rana grylia bark rot Chinese medicine composition, it is characterised in that the Rhizoma Polygonati Odorati
For 5-8 parts, Radix Trichosanthis are 4-8 parts.
5. the Chinese medicine composition of preventing and treating Rana grylia bark rot according to claim 1, it is characterised in that the Pu is public
English is 4-8 parts, and Herba Anoectochili roxburghii is 5-9 parts.
6. according to claim 1 preventing and treating Rana grylia bark rot Chinese medicine composition, it is characterised in that the emblic
Son is 4-6 parts, and Poria is 6-9 parts.
7. according to claim 1 preventing and treating Rana grylia bark rot Chinese medicine composition, it is characterised in that described wherein
The Radix Astragali is 5-7 parts, and Fructus Mori is 4-8 parts.
8. according to claim 1 preventing and treating Rana grylia bark rot medicine preparation method, it is characterised in that include successively
Following steps：
(1) by Abelmoschus esculentus fruit clean after at a temperature of 0-10 DEG C cold preservation 10-14 hours, by cold preservation after Abelmoschus esculentus fruit be cut into thin
Piece, carries out completing, dries；
(2) after other prepared slices of Chinese crude drugs remove impurity such as the Abelmoschus esculentus for making and Semen Trigonellae, cleaning, the fine powder that is ground into more than 60 mesh
Shape, being placed in 80-100 times of weight boiling water carries out the cooking of 60-70 minutes, and cooled and filtered obtains filtrate I, and melt cinder adds 2-3 times of weight
90-95% ethanol solution, extract 60-80 minutes at 40-45 DEG C, vacuum distillation obtains filtrate II, and the two mixing obtains final product extraction
Liquid；Leach that 0 liquid is spray-dried to make dry extract, add adjuvant, be first pressed into thick piece in advance, be then comminuted into granule；
(3) by the granule for making pack sterilizing.
9. the preparation method of Rana grylia bark rot medicine is prevented and treated according to claim 8, it is characterised in that step（2）Described
Adjuvant is Microcrystalline Cellulose, and Microcrystalline Cellulose with the weight ratio of dry extract is（10-14）：1.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106491952A (en) * | 2016-12-11 | 2017-03-15 | 衡阳县轩健农牧发展有限公司 | Protective agents of porcine skin disease and preparation method thereof |
-
2016
- 2016-12-06 CN CN201611110948.8A patent/CN106491961A/en active Pending
Non-Patent Citations (1)
| Title |
|---|
| 沈晓峰等: "黄芪在皮肤病治疗中的应用", 《新疆中医药》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106491952A (en) * | 2016-12-11 | 2017-03-15 | 衡阳县轩健农牧发展有限公司 | Protective agents of porcine skin disease and preparation method thereof |
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Application publication date: 20170315 |