CN106460033B - 使用核酸条形码分析与单细胞缔合的核酸 - Google Patents
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- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
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- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
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- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
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- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/60—Detection means characterised by use of a special device
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Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111683991.4A CN114717291A (zh) | 2013-12-30 | 2014-12-30 | 使用核酸条形码分析与单细胞缔合的核酸 |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361922012P | 2013-12-30 | 2013-12-30 | |
| US61/922,012 | 2013-12-30 | ||
| PCT/US2014/072898 WO2015103339A1 (en) | 2013-12-30 | 2014-12-30 | Analysis of nucleic acids associated with single cells using nucleic acid barcodes |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111683991.4A Division CN114717291A (zh) | 2013-12-30 | 2014-12-30 | 使用核酸条形码分析与单细胞缔合的核酸 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106460033A CN106460033A (zh) | 2017-02-22 |
| CN106460033B true CN106460033B (zh) | 2021-12-24 |
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Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111683991.4A Pending CN114717291A (zh) | 2013-12-30 | 2014-12-30 | 使用核酸条形码分析与单细胞缔合的核酸 |
| CN201480076617.XA Active CN106460033B (zh) | 2013-12-30 | 2014-12-30 | 使用核酸条形码分析与单细胞缔合的核酸 |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111683991.4A Pending CN114717291A (zh) | 2013-12-30 | 2014-12-30 | 使用核酸条形码分析与单细胞缔合的核酸 |
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Families Citing this family (189)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9424392B2 (en) | 2005-11-26 | 2016-08-23 | Natera, Inc. | System and method for cleaning noisy genetic data from target individuals using genetic data from genetically related individuals |
| US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
| US10316362B2 (en) | 2010-05-18 | 2019-06-11 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US20190010543A1 (en) | 2010-05-18 | 2019-01-10 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US12152275B2 (en) | 2010-05-18 | 2024-11-26 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| EP2854057B1 (en) | 2010-05-18 | 2018-03-07 | Natera, Inc. | Methods for non-invasive pre-natal ploidy calling |
| US12221653B2 (en) | 2010-05-18 | 2025-02-11 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US11332793B2 (en) | 2010-05-18 | 2022-05-17 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US11326208B2 (en) | 2010-05-18 | 2022-05-10 | Natera, Inc. | Methods for nested PCR amplification of cell-free DNA |
| US11322224B2 (en) | 2010-05-18 | 2022-05-03 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| US9677118B2 (en) | 2014-04-21 | 2017-06-13 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US11332785B2 (en) | 2010-05-18 | 2022-05-17 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| US11939634B2 (en) | 2010-05-18 | 2024-03-26 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US11339429B2 (en) | 2010-05-18 | 2022-05-24 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| US11408031B2 (en) | 2010-05-18 | 2022-08-09 | Natera, Inc. | Methods for non-invasive prenatal paternity testing |
| US9074251B2 (en) | 2011-02-10 | 2015-07-07 | Illumina, Inc. | Linking sequence reads using paired code tags |
| EP2670894B1 (en) | 2011-02-02 | 2017-11-29 | University Of Washington Through Its Center For Commercialization | Massively parallel continguity mapping |
| JP6153874B2 (ja) | 2011-02-09 | 2017-06-28 | ナテラ, インコーポレイテッド | 非侵襲的出生前倍数性呼び出しのための方法 |
| US9150852B2 (en) | 2011-02-18 | 2015-10-06 | Raindance Technologies, Inc. | Compositions and methods for molecular labeling |
| US11435358B2 (en) | 2011-06-23 | 2022-09-06 | Board Of Regents, The University Of Texas System | Single molecule peptide sequencing |
| GB2513024B (en) | 2012-02-27 | 2016-08-31 | Cellular Res Inc | A clonal amplification method |
| LT3305918T (lt) | 2012-03-05 | 2020-09-25 | President And Fellows Of Harvard College | Būdai, skirti epigenetinių sekų sudarymui |
| US10400280B2 (en) | 2012-08-14 | 2019-09-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US9951386B2 (en) * | 2014-06-26 | 2018-04-24 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10323279B2 (en) | 2012-08-14 | 2019-06-18 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10752949B2 (en) | 2012-08-14 | 2020-08-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10221442B2 (en) | 2012-08-14 | 2019-03-05 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| US10273541B2 (en) | 2012-08-14 | 2019-04-30 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US9701998B2 (en) | 2012-12-14 | 2017-07-11 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US11591637B2 (en) | 2012-08-14 | 2023-02-28 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| CN111748607B (zh) | 2012-08-14 | 2024-04-30 | 10X基因组学有限公司 | 微胶囊组合物及方法 |
| US20140100126A1 (en) | 2012-08-17 | 2014-04-10 | Natera, Inc. | Method for Non-Invasive Prenatal Testing Using Parental Mosaicism Data |
| US10533221B2 (en) | 2012-12-14 | 2020-01-14 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| CA2894694C (en) | 2012-12-14 | 2023-04-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| WO2014124338A1 (en) | 2013-02-08 | 2014-08-14 | 10X Technologies, Inc. | Polynucleotide barcode generation |
| CA2898456C (en) | 2013-03-13 | 2020-11-10 | Illumina, Inc. | Methods and compositions for nucleic acid sequencing |
| GB2525568B (en) * | 2013-03-15 | 2020-10-14 | Abvitro Llc | Single cell barcoding for antibody discovery |
| ES2857908T3 (es) | 2013-08-28 | 2021-09-29 | Becton Dickinson Co | Análisis masivamente paralelo de células individuales |
| WO2015054292A1 (en) | 2013-10-07 | 2015-04-16 | Cellular Research, Inc. | Methods and systems for digitally counting features on arrays |
| AU2014364926B2 (en) | 2013-12-20 | 2017-12-14 | Illumina, Inc. | Preserving genomic connectivity information in fragmented genomic DNA samples |
| SG11201605344YA (en) * | 2013-12-30 | 2016-07-28 | Atreca Inc | Analysis of nucleic acids associated with single cells using nucleic acid barcodes |
| CN114534806B (zh) | 2014-04-10 | 2024-03-29 | 10X基因组学有限公司 | 用于封装和分割试剂的流体装置、系统和方法及其应用 |
| AU2015249846B2 (en) | 2014-04-21 | 2021-07-22 | Natera, Inc. | Detecting mutations and ploidy in chromosomal segments |
| US20150298091A1 (en) | 2014-04-21 | 2015-10-22 | President And Fellows Of Harvard College | Systems and methods for barcoding nucleic acids |
| AU2015250034B2 (en) * | 2014-04-21 | 2019-06-20 | President And Fellows Of Harvard College | Systems and methods for barcoding nucleic acids |
| AU2015253299B2 (en) * | 2014-04-29 | 2018-06-14 | Illumina, Inc. | Multiplexed single cell gene expression analysis using template switch and tagmentation |
| US10975371B2 (en) | 2014-04-29 | 2021-04-13 | Illumina, Inc. | Nucleic acid sequence analysis from single cells |
| US20180173845A1 (en) | 2014-06-05 | 2018-06-21 | Natera, Inc. | Systems and Methods for Detection of Aneuploidy |
| US12312640B2 (en) | 2014-06-26 | 2025-05-27 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
| CN113249435B (zh) | 2014-06-26 | 2024-09-03 | 10X基因组学有限公司 | 分析来自单个细胞或细胞群体的核酸的方法 |
| CN106536756A (zh) | 2014-06-26 | 2017-03-22 | 10X基因组学有限公司 | 核酸序列的分析 |
| NZ730044A (en) * | 2014-09-15 | 2024-08-30 | Abvitro Llc | High-throughput nucleotide library sequencing |
| CA2964799A1 (en) | 2014-10-17 | 2016-04-21 | Illumina Cambridge Limited | Contiguity preserving transposition |
| PT3207134T (pt) * | 2014-10-17 | 2019-09-17 | Illumina Cambridge Ltd | Transposição que preserva a contiguidade |
| CN114807307A (zh) | 2014-10-29 | 2022-07-29 | 10X 基因组学有限公司 | 用于靶核酸测序的方法和组合物 |
| US9975122B2 (en) | 2014-11-05 | 2018-05-22 | 10X Genomics, Inc. | Instrument systems for integrated sample processing |
| SG11201705615UA (en) | 2015-01-12 | 2017-08-30 | 10X Genomics Inc | Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same |
| CN107250379B (zh) | 2015-02-19 | 2021-12-28 | 贝克顿迪金森公司 | 结合蛋白质组信息和基因组信息的高通量单细胞分析 |
| US11274343B2 (en) | 2015-02-24 | 2022-03-15 | 10X Genomics, Inc. | Methods and compositions for targeted nucleic acid sequence coverage |
| WO2016137973A1 (en) | 2015-02-24 | 2016-09-01 | 10X Genomics Inc | Partition processing methods and systems |
| ES2836802T3 (es) | 2015-02-27 | 2021-06-28 | Becton Dickinson Co | Códigos de barras moleculares espacialmente direccionables |
| WO2016138500A1 (en) * | 2015-02-27 | 2016-09-01 | Cellular Research, Inc. | Methods and compositions for barcoding nucleic acids for sequencing |
| EP4180535A1 (en) | 2015-03-30 | 2023-05-17 | Becton, Dickinson and Company | Methods and compositions for combinatorial barcoding |
| WO2016168584A1 (en) | 2015-04-17 | 2016-10-20 | President And Fellows Of Harvard College | Barcoding systems and methods for gene sequencing and other applications |
| EP4582556A3 (en) | 2015-04-23 | 2025-10-08 | Becton, Dickinson and Company | Methods and compositions for whole transcriptome amplification |
| EP3294906B1 (en) | 2015-05-11 | 2024-07-10 | Natera, Inc. | Methods for determining ploidy |
| US11124823B2 (en) | 2015-06-01 | 2021-09-21 | Becton, Dickinson And Company | Methods for RNA quantification |
| SG10201912283RA (en) * | 2015-08-28 | 2020-02-27 | Illumina Inc | Nucleic acid sequence analysis from single cells |
| ES2745694T3 (es) | 2015-09-11 | 2020-03-03 | Cellular Res Inc | Métodos y composiciones para normalización de biblioteca de ácido nucleico |
| EP3933047A1 (en) | 2015-09-24 | 2022-01-05 | AbVitro LLC | Affinity-oligonucleotide conjugates and uses thereof |
| CN108473984A (zh) * | 2015-11-04 | 2018-08-31 | 阿特雷卡公司 | 用于分析与单个细胞相关联的核酸的核酸条形码的组合组 |
| US20170141793A1 (en) * | 2015-11-13 | 2017-05-18 | Microsoft Technology Licensing, Llc | Error correction for nucleotide data stores |
| SG11201804086VA (en) | 2015-12-04 | 2018-06-28 | 10X Genomics Inc | Methods and compositions for nucleic acid analysis |
| WO2017117358A1 (en) * | 2015-12-30 | 2017-07-06 | Bio-Rad Laboratories, Inc. | Digital protein quantification |
| JP7179329B2 (ja) * | 2016-01-14 | 2022-11-29 | ヨーロピアン モレキュラー バイオロジー ラボラトリー | リガンド誘発型の細胞発現のマイクロ流体分析 |
| US20190085406A1 (en) | 2016-04-14 | 2019-03-21 | Guardant Health, Inc. | Methods for early detection of cancer |
| US11702702B2 (en) * | 2016-04-15 | 2023-07-18 | Predicine, Inc. | Systems and methods for detecting genetic alterations |
| WO2017181202A2 (en) | 2016-04-15 | 2017-10-19 | Natera, Inc. | Methods for lung cancer detection |
| AU2017261189B2 (en) | 2016-05-02 | 2023-02-09 | Becton, Dickinson And Company | Accurate molecular barcoding |
| WO2017197343A2 (en) | 2016-05-12 | 2017-11-16 | 10X Genomics, Inc. | Microfluidic on-chip filters |
| WO2017197338A1 (en) | 2016-05-13 | 2017-11-16 | 10X Genomics, Inc. | Microfluidic systems and methods of use |
| US10301677B2 (en) | 2016-05-25 | 2019-05-28 | Cellular Research, Inc. | Normalization of nucleic acid libraries |
| US20170342463A1 (en) * | 2016-05-25 | 2017-11-30 | Bio-Rad Laboratories, Inc. | Digital proximity assay |
| ES2979395T3 (es) | 2016-05-26 | 2024-09-25 | Becton Dickinson Co | Métodos de ajuste del recuento de etiquetas moleculares |
| US10202641B2 (en) | 2016-05-31 | 2019-02-12 | Cellular Research, Inc. | Error correction in amplification of samples |
| US10640763B2 (en) | 2016-05-31 | 2020-05-05 | Cellular Research, Inc. | Molecular indexing of internal sequences |
| CN105950612B (zh) * | 2016-07-08 | 2019-06-21 | 北京全式金生物技术有限公司 | 一种高效的dna接头连接方法 |
| CN109642219B (zh) | 2016-08-05 | 2021-02-26 | 生物辐射实验室股份有限公司 | 第二链引导 |
| WO2018031760A1 (en) * | 2016-08-10 | 2018-02-15 | Grail, Inc. | Methods of preparing dual-indexed dna libraries for bisulfite conversion sequencing |
| KR102522023B1 (ko) | 2016-09-26 | 2023-04-17 | 셀룰러 리서치, 인크. | 바코딩된 올리고뉴클레오티드 서열을 갖는 시약을 이용한 단백질 발현의 측정 |
| WO2018067517A1 (en) | 2016-10-04 | 2018-04-12 | Natera, Inc. | Methods for characterizing copy number variation using proximity-litigation sequencing |
| CN109983126A (zh) * | 2016-10-19 | 2019-07-05 | 10X基因组学有限公司 | 用于条形码化单个细胞或细胞群的核酸分子的方法和系统 |
| CN110312825A (zh) | 2016-10-24 | 2019-10-08 | 吉内恩福赛克公司 | 隐藏核酸内存在的信息 |
| GB201618485D0 (en) | 2016-11-02 | 2016-12-14 | Ucl Business Plc | Method of detecting tumour recurrence |
| WO2018089378A1 (en) | 2016-11-08 | 2018-05-17 | Cellular Research, Inc. | Methods for expression profile classification |
| US11608497B2 (en) | 2016-11-08 | 2023-03-21 | Becton, Dickinson And Company | Methods for cell label classification |
| EP3538662B1 (en) | 2016-11-10 | 2021-12-01 | Takara Bio USA, Inc. | Methods of producing amplified double stranded deoxyribonucleic acids and compositions and kits for use therein |
| US10011870B2 (en) * | 2016-12-07 | 2018-07-03 | Natera, Inc. | Compositions and methods for identifying nucleic acid molecules |
| CA3046379A1 (en) * | 2016-12-22 | 2018-06-28 | Illumina, Inc | Arrays with quality control tracers |
| US10550429B2 (en) | 2016-12-22 | 2020-02-04 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10815525B2 (en) | 2016-12-22 | 2020-10-27 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10011872B1 (en) * | 2016-12-22 | 2018-07-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| JP7104048B2 (ja) | 2017-01-13 | 2022-07-20 | セルラー リサーチ, インコーポレイテッド | 流体チャネルの親水性コーティング |
| US12264411B2 (en) | 2017-01-30 | 2025-04-01 | 10X Genomics, Inc. | Methods and systems for analysis |
| EP3545089B1 (en) | 2017-01-30 | 2022-03-09 | 10X Genomics, Inc. | Methods and systems for droplet-based single cell barcoding |
| EP3577232A1 (en) | 2017-02-01 | 2019-12-11 | Cellular Research, Inc. | Selective amplification using blocking oligonucleotides |
| LT3375889T (lt) * | 2017-03-17 | 2020-06-25 | Hifibio Sas | Vienos ląstelės analizė |
| AU2018252018A1 (en) * | 2017-04-14 | 2019-10-10 | Guardant Health, Inc. | Methods of attaching adapters to sample nucleic acids |
| CA3062287A1 (en) * | 2017-04-21 | 2018-10-25 | Mesa Biotech, Inc. | Fluidic test cassette |
| CN110168087B (zh) * | 2017-04-27 | 2023-11-14 | 深圳华大基因股份有限公司 | Dna标签及其应用 |
| US11584951B2 (en) | 2017-05-02 | 2023-02-21 | The University Of Tokyo | Method for integrally detecting nondestructive measurement information and genome-related information of one cell |
| SG11201910195WA (en) * | 2017-05-05 | 2019-11-28 | Scipio Bioscience | Methods for trapping and barcoding discrete biological units in hydrogel |
| US20180340169A1 (en) | 2017-05-26 | 2018-11-29 | 10X Genomics, Inc. | Single cell analysis of transposase accessible chromatin |
| CN116064732A (zh) | 2017-05-26 | 2023-05-05 | 10X基因组学有限公司 | 转座酶可接近性染色质的单细胞分析 |
| KR102790050B1 (ko) | 2017-06-05 | 2025-04-04 | 백톤 디킨슨 앤드 컴퍼니 | 단일 세포를 위한 샘플 인덱싱 |
| CN107502607A (zh) * | 2017-06-20 | 2017-12-22 | 浙江大学 | 一种大量组织、细胞样本mRNA的分子条形码标记、文库构建、测序的方法 |
| WO2019060716A1 (en) | 2017-09-25 | 2019-03-28 | Freenome Holdings, Inc. | SAMPLE EXTRACTION METHODS AND SYSTEMS |
| CN114525273B (zh) * | 2017-10-27 | 2025-01-28 | 10X基因组学有限公司 | 用于样品制备和分析的方法和系统 |
| CN111051523B (zh) | 2017-11-15 | 2024-03-19 | 10X基因组学有限公司 | 功能化凝胶珠 |
| US20190153438A1 (en) * | 2017-11-15 | 2019-05-23 | Viome, Inc. | Methods and compositions for preparing polynucleotide libraries |
| US10829815B2 (en) | 2017-11-17 | 2020-11-10 | 10X Genomics, Inc. | Methods and systems for associating physical and genetic properties of biological particles |
| WO2019104337A1 (en) | 2017-11-27 | 2019-05-31 | The Trustees Of Columbia University In The City Of New York | Rna printing and sequencing devices, methods, and systems |
| US20210371853A1 (en) * | 2017-12-09 | 2021-12-02 | Viome, Inc. | Methods for nucleic acid library creation |
| WO2019118926A1 (en) | 2017-12-14 | 2019-06-20 | Tai Diagnostics, Inc. | Assessing graft suitability for transplantation |
| CN111492068B (zh) * | 2017-12-19 | 2025-03-21 | 贝克顿迪金森公司 | 与寡核苷酸相关联的颗粒 |
| KR102014470B1 (ko) * | 2017-12-29 | 2019-08-26 | 한국과학기술원 | 핵산의 절단 및 중합연쇄반응 시스템 기반 등온 핵산증폭기술을 이용한 표적 rna 검출 방법 |
| WO2019133874A1 (en) * | 2017-12-31 | 2019-07-04 | Berkeley Lights, Inc. | General functional assay |
| WO2019147663A1 (en) * | 2018-01-24 | 2019-08-01 | Freenome Holdings, Inc. | Methods and systems for abnormality detection in the patterns of nucleic acids |
| SG11202007686VA (en) * | 2018-02-12 | 2020-09-29 | 10X Genomics Inc | Methods characterizing multiple analytes from individual cells or cell populations |
| US11639928B2 (en) | 2018-02-22 | 2023-05-02 | 10X Genomics, Inc. | Methods and systems for characterizing analytes from individual cells or cell populations |
| CN112074610A (zh) * | 2018-02-22 | 2020-12-11 | 10X基因组学有限公司 | 接合介导的核酸分析 |
| US20210087549A1 (en) * | 2018-02-23 | 2021-03-25 | Yale University | Single-cell freeze-thaw lysis |
| EP3762494B1 (en) * | 2018-03-07 | 2022-11-23 | Wisconsin Alumni Research Foundation | High throughput nucleic acid profiling of single cells |
| SG11202009889VA (en) | 2018-04-06 | 2020-11-27 | 10X Genomics Inc | Systems and methods for quality control in single cell processing |
| CA3090426A1 (en) | 2018-04-14 | 2019-10-17 | Natera, Inc. | Methods for cancer detection and monitoring by means of personalized detection of circulating tumor dna |
| EP4545647A3 (en) | 2018-05-03 | 2025-07-09 | Becton, Dickinson and Company | Molecular barcoding on opposite transcript ends |
| WO2019213294A1 (en) | 2018-05-03 | 2019-11-07 | Becton, Dickinson And Company | High throughput multiomics sample analysis |
| IL271454B2 (en) * | 2018-05-17 | 2025-04-01 | Illumina Inc | Rapid single-cell genetic sequencing with low Aggregation bias |
| US12234509B2 (en) | 2018-07-03 | 2025-02-25 | Natera, Inc. | Methods for detection of donor-derived cell-free DNA |
| EP3821010A4 (en) | 2018-07-12 | 2022-04-20 | Board of Regents, The University of Texas System | Molecular neighborhood detection by oligonucleotides |
| US20200032335A1 (en) | 2018-07-27 | 2020-01-30 | 10X Genomics, Inc. | Systems and methods for metabolome analysis |
| JP7631187B2 (ja) | 2018-08-17 | 2025-02-18 | ジェネンテック, インコーポレイテッド | In vitroトランスサイトーシスアッセイ |
| EP3844299A1 (en) * | 2018-08-28 | 2021-07-07 | Becton Dickinson and Company | Sample multiplexing using carbohydrate-binding and membrane-permeable reagents |
| EP4471156A3 (en) | 2018-10-01 | 2025-02-26 | Becton, Dickinson and Company | Determining 5' transcript sequences |
| JP2022504225A (ja) | 2018-10-05 | 2022-01-13 | ボード オブ リージェンツ,ザ ユニバーシティ オブ テキサス システム | 固相でのn末端ペプチド捕捉および解放の方法 |
| US20220010373A1 (en) | 2018-11-07 | 2022-01-13 | The University Of Tokyo | Method for detecting genome-related information of cell coexisting with at least one type of test substance |
| JP7618548B2 (ja) | 2018-11-08 | 2025-01-21 | ベクトン・ディキンソン・アンド・カンパニー | ランダムプライミングを使用した単一細胞の全トランスクリプトーム解析 |
| EP3891303A4 (en) * | 2018-12-07 | 2022-09-14 | Qiagen Sciences LLC | Methods for preparing cdna samples for rna sequencing, and cdna samples and uses thereof |
| US11459607B1 (en) * | 2018-12-10 | 2022-10-04 | 10X Genomics, Inc. | Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes |
| WO2020123384A1 (en) | 2018-12-13 | 2020-06-18 | Cellular Research, Inc. | Selective extension in single cell whole transcriptome analysis |
| CN111378557B (zh) | 2018-12-26 | 2023-06-06 | 财团法人工业技术研究院 | 用于产生液珠的管状结构及液珠产生方法 |
| WO2020150356A1 (en) | 2019-01-16 | 2020-07-23 | Becton, Dickinson And Company | Polymerase chain reaction normalization through primer titration |
| ES2989967T3 (es) | 2019-01-23 | 2024-11-28 | Becton Dickinson Co | Oligonucleótidos asociados con anticuerpos |
| US12071617B2 (en) | 2019-02-14 | 2024-08-27 | Becton, Dickinson And Company | Hybrid targeted and whole transcriptome amplification |
| WO2020214642A1 (en) | 2019-04-19 | 2020-10-22 | Becton, Dickinson And Company | Methods of associating phenotypical data and single cell sequencing data |
| EP3959330A4 (en) * | 2019-04-24 | 2023-07-05 | Molecular Assemblies, Inc. | HOMOPOLYMER ENCODED NUCLEIC ACID MEMORY |
| CN110241460A (zh) * | 2019-05-31 | 2019-09-17 | 南方医科大学南方医院 | 一种甄别独立样品自身交叉反应的免疫组库方法 |
| EP3980559A1 (en) | 2019-06-06 | 2022-04-13 | Natera, Inc. | Methods for detecting immune cell dna and monitoring immune system |
| CN120099137A (zh) | 2019-07-22 | 2025-06-06 | 贝克顿迪金森公司 | 单细胞染色质免疫沉淀测序测定 |
| CA3151004A1 (en) * | 2019-08-14 | 2021-02-18 | iRepertoire, Inc. | Probe-capture method for tcr alpha and beta chain vdj-recovery from oligo-dt reverse transcribed rna |
| CA3157359A1 (en) | 2019-10-10 | 2021-04-15 | 1859, Inc. | Methods and systems for microfluidic screening |
| CN114729350A (zh) | 2019-11-08 | 2022-07-08 | 贝克顿迪金森公司 | 使用随机引发获得用于免疫组库测序的全长v(d)j信息 |
| JP7107535B2 (ja) * | 2020-01-10 | 2022-07-27 | シンクサイト株式会社 | 新規細胞表現型スクリーニング方法 |
| US11512337B2 (en) | 2020-01-13 | 2022-11-29 | Fluent Biosciences Inc. | Emulsion based drug screening |
| CN115768558A (zh) | 2020-01-13 | 2023-03-07 | 福路伦特生物科学公司 | 用于单细胞基因谱分析的方法和系统 |
| JP2023511279A (ja) * | 2020-01-13 | 2023-03-17 | フルーエント バイオサイエンシーズ インコーポレイテッド | 単一細胞シーケンシング |
| US11649497B2 (en) | 2020-01-13 | 2023-05-16 | Becton, Dickinson And Company | Methods and compositions for quantitation of proteins and RNA |
| CN115175999A (zh) | 2020-01-22 | 2022-10-11 | 阿特雷卡公司 | 多个转录物的高通量连接 |
| US12416037B2 (en) * | 2020-01-24 | 2025-09-16 | Vanderbilt University | Methods for identification of ligand-blocking antibodies and for determining antibody potency |
| WO2021155057A1 (en) | 2020-01-29 | 2021-08-05 | Becton, Dickinson And Company | Barcoded wells for spatial mapping of single cells through sequencing |
| US12449419B1 (en) | 2020-02-12 | 2025-10-21 | 10X Genomics, Inc. | Methods for detecting binding of peptide-MHC monomers to T cells |
| WO2021173719A1 (en) | 2020-02-25 | 2021-09-02 | Becton, Dickinson And Company | Bi-specific probes to enable the use of single-cell samples as single color compensation control |
| US11866782B2 (en) | 2020-03-16 | 2024-01-09 | Fluent Biosciences Inc. | Multi-omic analysis in monodisperse droplets |
| US12428685B2 (en) | 2020-03-24 | 2025-09-30 | Illumina, Inc. | Viral detection using template emulsification |
| EP4150118A1 (en) | 2020-05-14 | 2023-03-22 | Becton Dickinson and Company | Primers for immune repertoire profiling |
| EP4158055B1 (en) | 2020-06-02 | 2024-03-27 | Becton, Dickinson and Company | Oligonucleotides and beads for 5 prime gene expression assay |
| US11932901B2 (en) | 2020-07-13 | 2024-03-19 | Becton, Dickinson And Company | Target enrichment using nucleic acid probes for scRNAseq |
| CA3189597A1 (en) | 2020-07-15 | 2022-01-20 | Fluent Biosciences Inc. | Tiered ligation oligos |
| WO2022026909A1 (en) | 2020-07-31 | 2022-02-03 | Becton, Dickinson And Company | Single cell assay for transposase-accessible chromatin |
| US11739443B2 (en) | 2020-11-20 | 2023-08-29 | Becton, Dickinson And Company | Profiling of highly expressed and lowly expressed proteins |
| US12392771B2 (en) | 2020-12-15 | 2025-08-19 | Becton, Dickinson And Company | Single cell secretome analysis |
| IL303800A (en) | 2020-12-18 | 2023-08-01 | Ionis Pharmaceuticals Inc | Compounds and methods for modulating factor xii |
| CA3202814A1 (en) * | 2020-12-22 | 2022-06-30 | Hifibio Sas | Microfluidic methods and systems |
| WO2022182682A1 (en) | 2021-02-23 | 2022-09-01 | 10X Genomics, Inc. | Probe-based analysis of nucleic acids and proteins |
| US20230048356A1 (en) * | 2021-03-10 | 2023-02-16 | Factorial Diagnostics, Inc. | Cell barcoding compositions and methods |
| EP4433600A4 (en) * | 2021-11-16 | 2025-10-08 | Pioneer Hi Bred Int | CORN EVENT DAS-01131-3 AND METHODS OF DETECTING THE SAME |
| EP4353825A1 (en) * | 2022-10-10 | 2024-04-17 | bisy GmbH | Modified promoter sequences |
| WO2025019326A2 (en) * | 2023-07-14 | 2025-01-23 | President And Fellows Of Harvard College | In situ amplification of rna |
| WO2025085431A1 (en) * | 2023-10-16 | 2025-04-24 | Bio-Rad Laboratories, Inc. | Enzymatic selection of nucleic acid |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2036989A1 (en) * | 2007-09-12 | 2009-03-18 | Institut Pasteur | Single cell based reporter assay to monitor gene expression patterns with high spatio-temporal resolution |
| WO2013134261A1 (en) * | 2012-03-05 | 2013-09-12 | President And Fellows Of Harvard College | Systems and methods for epigenetic sequencing |
Family Cites Families (31)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US5624711A (en) * | 1995-04-27 | 1997-04-29 | Affymax Technologies, N.V. | Derivatization of solid supports and methods for oligomer synthesis |
| US5928906A (en) | 1996-05-09 | 1999-07-27 | Sequenom, Inc. | Process for direct sequencing during template amplification |
| EP1510251B1 (en) | 2001-02-23 | 2006-09-06 | Japan Science and Technology Agency | Process and apparatus for producing microcapsules |
| US7198897B2 (en) * | 2001-12-19 | 2007-04-03 | Brandeis University | Late-PCR |
| US7976779B2 (en) * | 2002-06-26 | 2011-07-12 | California Institute Of Technology | Integrated LC-ESI on a chip |
| JP2007525963A (ja) * | 2003-06-20 | 2007-09-13 | イルミナ インコーポレイテッド | 全ゲノム増幅および遺伝型決定のための方法および組成物 |
| TWI333977B (en) | 2003-09-18 | 2010-12-01 | Symphogen As | Method for linking sequences of interest |
| CN100431679C (zh) | 2004-03-23 | 2008-11-12 | 独立行政法人科学技术振兴机构 | 微小液滴的生成方法及装置 |
| JOP20080381B1 (ar) | 2007-08-23 | 2023-03-28 | Amgen Inc | بروتينات مرتبطة بمولدات مضادات تتفاعل مع بروبروتين كونفيرتاز سيتيليزين ككسين من النوع 9 (pcsk9) |
| WO2010014820A2 (en) * | 2008-07-30 | 2010-02-04 | Life Technologies Corporation | Particles for use in supported nucleic acid ligation and detection sequencing |
| US9625454B2 (en) | 2009-09-04 | 2017-04-18 | The Research Foundation For The State University Of New York | Rapid and continuous analyte processing in droplet microfluidic devices |
| EP2420579A1 (en) * | 2010-08-17 | 2012-02-22 | QIAGEN GmbH | Helicase dependent isothermal amplification using nicking enzymes |
| EP2614154B1 (en) * | 2010-09-10 | 2014-12-17 | New England Biolabs, Inc. | Method for reducing adapter-dimer formation |
| ES2730951T3 (es) | 2010-10-08 | 2019-11-13 | Harvard College | Secuenciación inmune de alto rendimiento |
| EP2625320B1 (en) * | 2010-10-08 | 2019-03-27 | President and Fellows of Harvard College | High-throughput single cell barcoding |
| DK2652155T3 (en) | 2010-12-16 | 2017-02-13 | Gigagen Inc | Methods for Massive Parallel Analysis of Nucleic Acids in Single Cells |
| US9150852B2 (en) * | 2011-02-18 | 2015-10-06 | Raindance Technologies, Inc. | Compositions and methods for molecular labeling |
| WO2012129363A2 (en) | 2011-03-24 | 2012-09-27 | President And Fellows Of Harvard College | Single cell nucleic acid detection and analysis |
| US20140141442A1 (en) * | 2011-04-05 | 2014-05-22 | Institut National De La Sante Et De La Recherche Medicale (Inserm) | Linear dna amplification |
| DK2702146T3 (en) | 2011-04-28 | 2019-03-11 | Univ Leland Stanford Junior | IDENTIFICATION OF POLYNUCLEOTIDES CONNECTED WITH A SAMPLE |
| WO2013096643A1 (en) | 2011-12-23 | 2013-06-27 | Gigagen | Methods and apparatuses for droplet mixing |
| WO2013126741A1 (en) * | 2012-02-24 | 2013-08-29 | Raindance Technologies, Inc. | Labeling and sample preparation for sequencing |
| NO2694769T3 (enExample) * | 2012-03-06 | 2018-03-03 | ||
| EP2852682B1 (en) * | 2012-05-21 | 2017-10-04 | Fluidigm Corporation | Single-particle analysis of particle populations |
| DK2861760T3 (da) | 2012-06-15 | 2020-03-09 | Univ Texas | Sekventering med høj kapacitet af flere udskrifter af en enkelt celle |
| WO2014108850A2 (en) * | 2013-01-09 | 2014-07-17 | Yeda Research And Development Co. Ltd. | High throughput transcriptome analysis |
| CN115558661A (zh) * | 2013-08-23 | 2023-01-03 | 路德维格癌症研究院 | 用于使用模板转换反应进行cDNA合成和单细胞转录组概况分析的方法和组合物 |
| GB201317301D0 (en) * | 2013-09-30 | 2013-11-13 | Linnarsson Sten | Method for capturing and encoding nucleic acid from a plurality of single cells |
| SG11201605344YA (en) | 2013-12-30 | 2016-07-28 | Atreca Inc | Analysis of nucleic acids associated with single cells using nucleic acid barcodes |
| US20150298091A1 (en) * | 2014-04-21 | 2015-10-22 | President And Fellows Of Harvard College | Systems and methods for barcoding nucleic acids |
-
2014
- 2014-12-30 SG SG11201605344YA patent/SG11201605344YA/en unknown
- 2014-12-30 PL PL14830928.9T patent/PL3089822T3/pl unknown
- 2014-12-30 CA CA2935122A patent/CA2935122C/en active Active
- 2014-12-30 WO PCT/US2014/072898 patent/WO2015103339A1/en not_active Ceased
- 2014-12-30 PT PT148309289T patent/PT3089822T/pt unknown
- 2014-12-30 US US14/586,857 patent/US9580736B2/en active Active
- 2014-12-30 CN CN202111683991.4A patent/CN114717291A/zh active Pending
- 2014-12-30 CN CN201480076617.XA patent/CN106460033B/zh active Active
- 2014-12-30 DK DK14830928.9T patent/DK3089822T3/da active
- 2014-12-30 JP JP2016544526A patent/JP6608368B2/ja active Active
- 2014-12-30 ES ES14830928T patent/ES2912183T3/es active Active
- 2014-12-30 AU AU2014373757A patent/AU2014373757B2/en active Active
- 2014-12-30 EP EP22165189.6A patent/EP4094834A1/en active Pending
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- 2014-12-30 KR KR1020227028110A patent/KR20220119751A/ko not_active Ceased
- 2014-12-30 SG SG10201807112XA patent/SG10201807112XA/en unknown
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2017
- 2017-02-08 US US15/428,064 patent/US10316345B2/en active Active
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2019
- 2019-05-03 US US16/402,626 patent/US20200123582A1/en not_active Abandoned
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2022
- 2022-04-08 US US17/716,617 patent/US20220243240A1/en active Pending
- 2022-06-17 US US17/842,968 patent/US20220389472A1/en not_active Abandoned
- 2022-06-17 US US17/842,966 patent/US20220389471A1/en not_active Abandoned
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2024
- 2024-02-07 US US18/435,438 patent/US20250059576A1/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2036989A1 (en) * | 2007-09-12 | 2009-03-18 | Institut Pasteur | Single cell based reporter assay to monitor gene expression patterns with high spatio-temporal resolution |
| WO2013134261A1 (en) * | 2012-03-05 | 2013-09-12 | President And Fellows Of Harvard College | Systems and methods for epigenetic sequencing |
Also Published As
| Publication number | Publication date |
|---|---|
| KR20160108377A (ko) | 2016-09-19 |
| PL3089822T3 (pl) | 2022-09-19 |
| EP3089822A1 (en) | 2016-11-09 |
| US20250059576A1 (en) | 2025-02-20 |
| CA2935122C (en) | 2023-09-19 |
| US20170369921A1 (en) | 2017-12-28 |
| JP6608368B2 (ja) | 2019-11-20 |
| US9580736B2 (en) | 2017-02-28 |
| SG11201605344YA (en) | 2016-07-28 |
| DK3089822T3 (da) | 2022-05-02 |
| KR20220119751A (ko) | 2022-08-30 |
| AU2014373757B2 (en) | 2019-12-12 |
| US20200123582A1 (en) | 2020-04-23 |
| US10316345B2 (en) | 2019-06-11 |
| SG10201807112XA (en) | 2018-09-27 |
| EP4094834A1 (en) | 2022-11-30 |
| US20220243240A1 (en) | 2022-08-04 |
| ES2912183T3 (es) | 2022-05-24 |
| AU2014373757A1 (en) | 2016-07-07 |
| CA2935122A1 (en) | 2015-07-09 |
| CN106460033A (zh) | 2017-02-22 |
| JP2017506877A (ja) | 2017-03-16 |
| CN114717291A (zh) | 2022-07-08 |
| US20220389472A1 (en) | 2022-12-08 |
| KR102433825B1 (ko) | 2022-08-31 |
| US20150329891A1 (en) | 2015-11-19 |
| US20220389471A1 (en) | 2022-12-08 |
| WO2015103339A1 (en) | 2015-07-09 |
| EP3089822B1 (en) | 2022-04-06 |
| PT3089822T (pt) | 2022-05-06 |
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