CN106431669A - Method for planting selenium-rich peanuts - Google Patents
Method for planting selenium-rich peanuts Download PDFInfo
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- CN106431669A CN106431669A CN201610870006.3A CN201610870006A CN106431669A CN 106431669 A CN106431669 A CN 106431669A CN 201610870006 A CN201610870006 A CN 201610870006A CN 106431669 A CN106431669 A CN 106431669A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
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- C05—FERTILISERS; MANUFACTURE THEREOF
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- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- C—CHEMISTRY; METALLURGY
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- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
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Abstract
The invention discloses a method for planting selenium-rich peanuts. The method comprises the following steps of 1, soil preparation and fertilization; 2, seed selecting and sowing; 3, field management, and further comprises the step that in the peanut blooming acicula forming period, the fruiting period and the later growth and development period, a cultivation nutrient solution is spayed to leaf surfaces. The cultivation nutrient solution is prepared through the following method which includes the steps that 1, raw materials are prepared, wherein 100 parts of pure water, 0.6-1.0 part of L-selenium methionine, 1.5-2.5 parts of water-soluble compound fertilizer with NPK being 15-15-15 and 0.1-0.3 part of ethoxy fibers are prepared; 2, the raw materials in the step 1 are mixed and then put in a high-pressure tank, the pressure is increased to 4 kg/cm<2>, the pressure is made to be even and kept for 2 min to 3 min, then normal pressure is restored within 1 s to 3 s, pulp is obtained, the pulp is inoculated with bacillus subtilis liquid strains cultured to be mature at the temperature of 25 DEG C according to the volume percent of 5%-10%, the materials are put in a fermentation tank, sterile air is introduced into the fermentation tank, stirring and fermentation are carried out, after fermentation is completed, the temperature is quickly raised to 70 DEG C to be kept for 0.5 h to 1 h, then inactivation is carried out, and the selenium-rich peanuts are obtained.
Description
Technical field
The present invention relates to the cultural method of peanut cultivation technical field, more particularly, to selenium-rich peanuts.
Background technology
Semen arachidis hypogaeae (peanut), original name Semen arachidis hypogaeae (scientific name：Arachis hypogaea Linn.), be China's abundance,
Eat widely a kind of nut, also known as " peanut ", " mud bean " etc..Semen arachidis hypogaeae fruit contains protein, fat, saccharide, vitamin
A, vitamin B6, Vitamin E, vitamin K, and the nutritional labeling such as mineral calcium, phosphorus, ferrum, the amino containing 8 kinds of needed by human body
Acid and unsaturated fatty acid, containing materials such as lecithin, choline, carotene, crude fibres.Fat content is 44-45%, protein
Content is 24-36%, and sugar content is 20% about.Containing abundant vitamin B2, PP, A, D, E, calcium and ferrum etc..And contain sulfur
The multivitamins such as amine element, riboflavin, nicotinic acid, have the brain cell development promoting people, the effect of hypermnesis.Semen arachidis hypogaeae is China
Important industrial crops, rich in fat and protein, and contain the multivitamin such as thiamine, riboflavin, nicotinic acid, mineral
Content is also very abundant, particularly contains the necessary aminoacid of human body, has promotion brain cell development, the function of hypermnesis, both may be used
Edible, also feedstuff can use, be the important raw material of industry, be large exporting again.Of many uses, national economy accounts for weight
Want status.
For a long time, peanut cultivation does not have the high-yield culturing mode of system perfecting, makes peanut cultivation close not high, and divulges information
Light transmission condition is poor, and Semen arachidis hypogaeae can not carry out photosynthesis well, adds trace element deficiency and peanut nutrition growth imbalance, causes
Semen arachidis hypogaeae per mu yield level is made to be restricted, 200-300 kilogram of usual mu.
Selenium is trace element needed by human.Selenium participates in multiple selenoenzymes and Selenonic protein in synthesized human.Wherein paddy Guang
Sweet peptide peroxidase, is catalyzed hydroperoxides in vivo or lipid peroxide is changed into water or various alcohols, eliminates
Free radical, to biomembranous attack, protects biomembrane from oxidative damage；Selenium participates in constituting the de- iodine enzyme of iodate thyroid amino acid.?
Internal selenium and Vitamin E are worked in coordination with, and can protect cell membrane, prevent the oxidation of unsaturated fatty acid.Trace selenium has protective effect on cancer risk
And liver-protective effect.Selenium can improve human immunity, promotes the propagation of lymphocyte and the synthesis of antibody and immunoglobulin.
Selenium has the obvious effect suppressing and protecting to kinds cancers such as colon cancer, skin carcinoma, hepatocarcinoma, breast carcinoma, and it is in body
Mesostate methyl enol there is stronger active anticancer.Selenium is sought with Vitamin E, garlicin, linoleic acid, germanium, zinc etc.
Foster element has collaborative antioxidative effect, increases antioxidant activity.Meanwhile, selenium has the work mitigating and alleviating heavy metal toxicity
With.
Scientific circles' research finds, the height of blood selenium level is closely bound up with the generation of cancer.Substantial amounts of survey data explanation, one
Individual area food and the height of Selenium in Soils content have direct relation with the sickness rate of cancer, for example：This regional food and soil
Se content in earth is high, and the M ＆ M of cancer is just low, conversely, this regional cancer morbidity and mortality rate are just
Height, the generation close relation of true explanation selenium and cancer.Simultaneously scientific circles also identify selenium have prophylaxis of cancer effect, be
" king of anti-cancer " of body trace element.Because inorganic selenium salt toxicity is larger, forbid in food and feedstuff within 1993 in Japan
Middle interpolation, supports using organic form selenium such as the higher Selenonic protein of safety, aminoacid, or Se-rich farm products.
The research to selenium-rich peanuts at present, such as Chinese patent CN102643713B, Biological selenium-rich aromatic peanut oil
And its production method, describe the Semen arachidis hypogaeae that it obtains, the organic selenium constituent content of Semen arachidis hypogaeae reaches 0.490mg/kg after testing,
And common peanut core organic selenium constituent content is 0.014mg/kg, it is 35 times of common peanut core.And for example Chinese patent
CN103988685B, a kind of high yield cultivating method of selenium-rich peanuts, the Se content in institute's yield peanut is stablized in 0.18-0.2mg/
Kg, and in the Semen arachidis hypogaeae of conventional method cultivation, Se content is only less than 0.05, considerably increases the health-care effect of Semen arachidis hypogaeae.
Content of the invention
It is an object of the invention to provide the cultural method of selenium-rich peanuts, reach realize Semen arachidis hypogaeae high yield increase income, selenium element content
Stable effect.
The cultural method of selenium-rich peanuts of the present invention, comprises the steps：
1) site preparation and fertilization：Suitable soil is selected to turn over after spreading fertilizer over the fields the mixed fertilizer that becomes thoroughly decomposed ridging, ploughed depth 25-30cm；
2) seed selection sowing：Select full pair of a kind of fruit, such as apple, pear, etc. peanut pod, shine fruit prior to seeding and peel off after 2-3 days, grain-by-grain seed selection after peeling off
Go out the big seed of perfect kernel as kernel, kernel is carried out beginning sowing in good time after dressing seed, depth of planting 3-7cm；
3) field management：Weeding, split application, irrigation and drainage and PCO before after planting carrying out bud, and fit
When harvest；
Also include, bloom lower pin phase, fruiting period and growth promoter later stage foliage-spray cultivating nutrient solution respectively in Semen arachidis hypogaeae, institute
State cultivating nutrient solution, be prepared by the following preparation method：
1. raw material prepares：Prepare 100 parts of pure water, L- Selenomethionine 0.6-1.0 part, the water solublity for 15-15-15 for the NPK
Compound fertilizer's 1.5-2.5 part, hydroxyethyl cellulose 0.1-0.3 part；
2., after raw material 1. mixes by step, put in pressure pan, be forced into 4kg/cm2So as to pressure is uniformly, and keep
2-3min, then recovers normal pressure in 1-3s, obtains serosity, presses percent by volume at 25 DEG C and accesses culture maturation for 5-10%
Bacillus subtilises liquid spawn, puts in fermentation tank, is passed through filtrated air in fermentation tank, is stirred fermenting, fermentation ends
Afterwards, rapidly temperature is risen to 70 DEG C and keep 0.5-1.0h inactivation, obtain.
Step 1 of the present invention) described in mixed fertilizer of becoming thoroughly decomposed, preparation method preferably by every mu needs fertilizer 1500kg and calcium
Magnesium-phosphorus fertilizer 50kg 10d mixing compost prior to seeding, prior to seeding 2d by carbamide 5kg, potassium sulfate 10kg and compound fertilizer lOkg mix
Continue compost until turning over before sowing after the fertilizer mixing of compost.
Step 2) described in dressing seed, preferably 50% carbendazol wettable powder presses 0.3% and kernel of kernel weight
Mixed, described begins sowing in good time, refer to be sowed respectively at spring and autumn, the wherein sowing time in spring is selected vertical
Before and after Spring Festival gas, and away from sowing when at least continuous 5d average temperature of the earth's surface 4-6cm depth soil moisture is more than 15 DEG C, planted in fall
Selection of time is in late July to early August.
Step 2) described sowing, preferably sow spacing in the rows 18-22cm, line-spacing 20-25cm, 4-6 row, every cave are sowed in each ridge face
Broadcast 2-3 grain kernel, thickness of sowing 1.8-2.0 ten thousand Seedlings/mu, depth of planting 3-7cm, and suppressed in after planting.
Step 3) described in field management, be to carry out with reference to fertilization strategy when peanut seedling stem grows 3-5 blade
Intertillage is loosened the soil, and described fertilising is to impose Semen arachidis hypogaeae composite fertilizer special lOkg according to every mu, or the consumption of carbamide 4kg and potassium sulfate 5kg
Spread fertilizer over the fields；Also include florescence fertilising, bloom 10-15d in Semen arachidis hypogaeae respectively and Semen arachidis hypogaeae blooms lower pin early stage according to 20-25kg/ mu
Consumption spread fertilizer over the fields plant ash and Calx, and carry out cultivation and banking after spreading fertilizer over the fields；Before described bud, herbicidal methods are：After planting 3-4 days
It is sprayed on ridge according to every mu of sown area with after 80% isopropyl methylamine cream 90-1358 and N,N'-dimethyl-.gamma..gamma.'-dipyridylium 75m1 and water 50kg mixture
Face and ridge both sides.
Spray, so that blade face uniform fold to be defined after described cultivating nutrient solution, preferably 3-5 times of dilution.
The 1. described raw material of step, preferably 100 parts of pure water, 0.8 part of L- Selenomethionine, NPK are the water-soluble of 15-15-15
2.0 parts of compound fertilizer of property, 0.2 part of hydroxyethyl cellulose, described L- Selenomethionine, i.e. L- selenomethionine, it is also called selenium egg ammonia
Acid.
The step 2. preferred 250r/min of described speed of agitator, described fermentation, preferable temperature keeps 35-37 DEG C, fermentation time
For 3-4h.
The cultural method of described bacillus subtilises liquid spawn is：Using triangular flask strain cultivation, below attach most importance to
Amount percent, by the albumen processing slurry 30.0-35.0%, KH2P041.0%, MgS042.0%, NaCl0.5% put into three
In the bottle of angle, stir, adjust pH to 5.6,125 DEG C of sterilizing 10min, be cooled to 25 DEG C, access the hay through slant culture
Bacillus, 28 DEG C of shaking table cultures 60-100h, for fermentation tank inoculation.
Compared to existing technology, it is an advantage of the current invention that：
1st, Selenium-rich nutrient solution of the prior art, usually using inorganic selenium, particularly sodium selenite and sodium selenate, sub- selenium
Sour potassium and potassium selenate etc., obtain from the side-product of metal mineral reserves, and inorganic selenium have larger toxicity, and are difficult to be absorbed, as
Nutritional solution, assimilation effect is bad.The Selenomethionine that the present invention uses, is a kind of organic selenium source, compared with inorganic selenium, seleno egg ammonia
Acid has safe and efficient advantage, and it can substitute some methionine and be attached to the reversible selenium storage of formation one on body protein
Storehouse, and there is not toxic hazard.
2nd, Selenomethionine is that in nature, selenium is present in one of plant and feed grain seleno amino with organic form
The plant growings such as acid, frumentum and forage crop are not necessarily required to Selenomethionine, but with Methionine synthesis they can utilize ring
Selenite particularly in soil and selenate synthesis Selenomethionine in border.Available selenium in soil is more, in plant
Selenomethionine content is higher.At present, Selenomethionine is mainly derived from natural selenium yeast, is being primarily present of organic selenium in selenium yeast
Form, but Selenomethionine has dense yeast taste, directly water-soluble rear sprinkling, and absorption efficiency is low and influences whether finally to produce
The taste and flavor of product.Bacillus subtillis, are one kind of Bacilluss, and bacillus subtilises thalline itself synthesizes alphalise starch
The enzymes such as enzyme, protease, Digestive Enzyme, cellulase, are equivalent to compound enzyme, and the present invention is mixed withered by Selenomethionine, compound fertilizer
Careless bacilluss after fermentation, is fermented using the zymasises that Selenomethionine product carries, and is on the one hand beneficial to selenium element and enters Semen arachidis hypogaeae,
On the other hand with Methionine synthesis they can be using the selenite particularly in soil and selenate synthesis selenium in environment
Methionine, can also be effectively improved, dissolve yeast flavor.
3rd, the HIGH PRESSURE TREATMENT that the food sterilization stage uses is applied to the compounding stage by the present invention, by " being forced into
4kg/cm2So as to pressure is uniformly, and keep 2-3min, in 1-3s, then recover normal pressure ", by moment, pressure is reduced to 0,
On the one hand can be changed to sterilize by the moment of pressure, on the other hand can effectively refine, disperse Selenomethionine and compound fertilizer,
Advantageously in the absorption of Semen arachidis hypogaeae.
4th, the present invention with the addition of hydroxyethyl cellulose in nutritional solution, has good thickening, suspension, dispersion, emulsifying, glues
The characteristics such as conjunction, film forming, protection moisture and offer protecting colloid, using so that Selenomethionine fully sticks in nutritional solution
On blade face, branch, beneficial to the absorption to nutritional labeling for the blade face, the Semen arachidis hypogaeae being obtained using the technology of the present invention, Semen arachidis hypogaeae after testing
Organic selenium constituent content reach 0.65-0.98mg/kg.
Specific embodiment
With embodiment, the invention will be further described below, but the invention is not limited in these embodiments.
Embodiment 1：
The cultural method of selenium-rich peanuts, comprises the steps：
1) site preparation and fertilization：Suitable soil is selected to turn over after spreading fertilizer over the fields the mixed fertilizer that becomes thoroughly decomposed ridging, ploughed depth 25-30cm；
2) seed selection sowing：Select full pair of a kind of fruit, such as apple, pear, etc. peanut pod, shine fruit prior to seeding and peel off after 2-3 days, grain-by-grain seed selection after peeling off
Go out the big seed of perfect kernel as kernel, kernel is carried out beginning sowing in good time after dressing seed, depth of planting 3-7cm；
3) field management：Weeding, split application, irrigation and drainage and PCO before after planting carrying out bud, and fit
When harvest；
Also include, bloom lower pin phase, fruiting period and growth promoter later stage foliage-spray cultivating nutrient solution respectively in Semen arachidis hypogaeae, institute
State cultivating nutrient solution, be prepared by the following preparation method：
1. raw material prepares：Prepare 100 parts of pure water, 0.6 part of L- Selenomethionine, the Water Soluble Compound for 15-15-15 for the NPK
Fertile 1.5 parts, 0.3 part of hydroxyethyl cellulose；
2., after raw material 1. mixes by step, put in pressure pan, be forced into 4kg/cm2So as to pressure is uniformly, and keep
2-3min, then recovers normal pressure in 1-3s, obtains serosity, is ripe withered of 5% access culture at 25 DEG C by percent by volume
Careless bacillus cereuss liquid strain, puts in fermentation tank, is passed through filtrated air in fermentation tank, is stirred fermenting, speed of agitator
250r/min, fermentation time is 3h, and temperature keeps 35-37 DEG C, after fermentation ends, rapidly temperature is risen to 70 DEG C and keeps 0.5h to go out
Live, obtain.
Embodiment 2：
1) site preparation and fertilization：Suitable soil is selected to turn over after spreading fertilizer over the fields the mixed fertilizer that becomes thoroughly decomposed ridging, ploughed depth 25-30cm；
2) seed selection sowing：Select full pair of a kind of fruit, such as apple, pear, etc. peanut pod, shine fruit prior to seeding and peel off after 2-3 days, grain-by-grain seed selection after peeling off
Go out the big seed of perfect kernel as kernel, kernel is carried out beginning sowing in good time after dressing seed, depth of planting 3-7cm；
3) field management：Weeding, split application, irrigation and drainage and PCO before after planting carrying out bud, and fit
When harvest；
Also include, bloom lower pin phase, fruiting period and growth promoter later stage foliage-spray cultivating nutrient solution respectively in Semen arachidis hypogaeae, institute
State cultivating nutrient solution, be prepared by the following preparation method：
1. raw material prepares：Prepare 100 parts of pure water, 1.0 parts of L- Selenomethionine, the Water Soluble Compound for 15-15-15 for the NPK
Fertile 2.5 parts, 0.1 part of hydroxyethyl cellulose；
2., after raw material 1. mixes by step, put in pressure pan, be forced into 4kg/cm2So as to pressure is uniformly, and keep
2-3min, then recovers normal pressure in 1-3s, obtains serosity, is ripe withered of 10% access culture at 25 DEG C by percent by volume
Careless bacillus cereuss liquid strain, puts in fermentation tank, is passed through filtrated air in fermentation tank, is stirred fermenting, speed of agitator
250r/min, fermentation time is 4h, and temperature keeps 35-37 DEG C, after fermentation ends, rapidly temperature is risen to 70 DEG C and keeps 1.0h to go out
Live, obtain.
Embodiment 3：
1) site preparation and fertilization：Suitable soil is selected to turn over after spreading fertilizer over the fields the mixed fertilizer that becomes thoroughly decomposed ridging, ploughed depth 25-30cm；
2) seed selection sowing：Select full pair of a kind of fruit, such as apple, pear, etc. peanut pod, shine fruit prior to seeding and peel off after 2-3 days, grain-by-grain seed selection after peeling off
Go out the big seed of perfect kernel as kernel, kernel is carried out beginning sowing in good time after dressing seed, depth of planting 3-7cm；
3) field management：Weeding, split application, irrigation and drainage and PCO before after planting carrying out bud, and fit
When harvest；
Also include, bloom lower pin phase, fruiting period and growth promoter later stage foliage-spray cultivating nutrient solution respectively in Semen arachidis hypogaeae, institute
State cultivating nutrient solution, be prepared by the following preparation method：
1. raw material prepares：Prepare 100 parts of pure water, 0.8 part of L- Selenomethionine, the Water Soluble Compound for 15-15-15 for the NPK
Fertile 2.0 parts, 0.2 part of hydroxyethyl cellulose；
2., after raw material 1. mixes by step, put in pressure pan, be forced into 4kg/cm2So as to pressure is uniformly, and keep
2-3min, then recovers normal pressure in 1-3s, obtains serosity, is ripe withered of 8% access culture at 25 DEG C by percent by volume
Careless bacillus cereuss liquid strain, puts in fermentation tank, is passed through filtrated air in fermentation tank, is stirred fermenting, speed of agitator
250r/min, fermentation time is 3h, and temperature keeps 35-37 DEG C, after fermentation ends, rapidly temperature is risen to 70 DEG C and keeps 0.5h to go out
Live, obtain.
Comparative example：
1) site preparation and fertilization：Suitable soil is selected to turn over after spreading fertilizer over the fields the mixed fertilizer that becomes thoroughly decomposed ridging, ploughed depth 25-30cm；
2) seed selection sowing：Select full pair of a kind of fruit, such as apple, pear, etc. peanut pod, shine fruit prior to seeding and peel off after 2-3 days, grain-by-grain seed selection after peeling off
Go out the big seed of perfect kernel as kernel, kernel is carried out beginning sowing in good time after dressing seed, depth of planting 3-7cm；
3) field management：Weeding, split application, irrigation and drainage and PCO before after planting carrying out bud, and fit
When harvest；
Also include, bloom lower pin phase, fruiting period and growth promoter later stage foliage-spray cultivating nutrient solution respectively in Semen arachidis hypogaeae, institute
State cultivating nutrient solution, be prepared by the following preparation method：
1. raw material prepares：Prepare 100 parts of pure water, 0.8 part of L- Selenomethionine, the Water Soluble Compound for 15-15-15 for the NPK
Fertile 2.0 parts, 0.2 part of hydroxyethyl cellulose,
2. by step, raw material 1. obtains after mixing.
Experimental example：
The three batch Semen arachidis hypogaeaes being obtained using the embodiment of the present invention 3, the organic selenium constituent content difference of Semen arachidis hypogaeae after testing
It is 0.73mg/kg, 1.05mg/kg, 0.84mg/kg；
The three batch Semen arachidis hypogaeaes being obtained using comparative example, after testing the organic selenium constituent content of Semen arachidis hypogaeae be respectively
0.15mg/kg、0.12mg/kg、0.09mg/kg.
Claims (4)
1. the cultural method of selenium-rich peanuts is it is characterised in that comprise the steps：
1) site preparation and fertilization：Suitable soil is selected to turn over after spreading fertilizer over the fields the mixed fertilizer that becomes thoroughly decomposed ridging, ploughed depth 25-30cm；
2) seed selection sowing：Select full pair of a kind of fruit, such as apple, pear, etc. peanut pod, shine fruit prior to seeding and peel off after 2-3 days, grain-by-grain seed selection after peeling off has gone out
The big seed of granulate, as kernel, kernel is carried out beginning sowing in good time after dressing seed, depth of planting 3-7cm；
3) field management：Weeding, split application, irrigation and drainage and PCO before after planting carrying out bud, and receive in good time
Obtain；
Also include, bloom lower pin phase, fruiting period and growth promoter later stage foliage-spray cultivating nutrient solution respectively in Semen arachidis hypogaeae, described cultivation
Cultivation nutrient fluid, is prepared by the following preparation method：
1. raw material prepares：Prepare 100 parts of pure water, L- Selenomethionine 0.6-1.0 part, the Water Soluble Compound for 15-15-15 for the NPK
Fertile 1.5-2.5 part, hydroxyethyl cellulose 0.1-0.3 part；
2., after raw material 1. mixes by step, put in pressure pan, be forced into 4kg/cm2So as to pressure is uniformly, and keep 2-
3min, then recovers normal pressure in 1-3s, obtains serosity, presses percent by volume at 25 DEG C and accesses the withered of culture maturation for 5-10%
Careless bacillus cereuss liquid strain, puts in fermentation tank, is passed through filtrated air in fermentation tank, is stirred fermenting, after fermentation ends,
Rapidly temperature is risen to 70 DEG C and keep 0.5-1.0h inactivation, obtain.
2. selenium-rich peanuts according to claim 1 cultural method it is characterised in that：The 1. described raw material of step, is pure
100 parts of water purification, 0.8 part of L- Selenomethionine, NPK are 2.0 parts of the water soluble composite fertilizer of 15-15-15,0.2 part of hydroxyethyl cellulose.
3. selenium-rich peanuts according to claim 1 cultural method it is characterised in that：The 2. described speed of agitator of step
250r/min, described fermentation, temperature keeps 35-37 DEG C, and fermentation time is 3-4h.
4. selenium-rich peanuts according to claim 3 cultural method it is characterised in that：Step 2. described bacillus subtilises
The cultural method of liquid spawn is：Using triangular flask strain cultivation, below for percetage by weight, by the egg albumen powder processing
Slurry 30.0-35.0%, KH2PO41.0%, MgSO42.0%, NaCl0.5% put in triangular flask, stir, adjust pH to
5.6,125 DEG C of sterilizing 10min, are cooled to 25 DEG C, access the Bacillus subtilis strain through slant culture, 28 DEG C of shaking table cultures
60-100h, for fermentation tank inoculation.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108739200A (en) * | 2018-06-11 | 2018-11-06 | 广西健美乐食品有限公司 | A kind of implantation methods of selenium-rich peanuts |
| CN110301318A (en) * | 2019-08-09 | 2019-10-08 | 广西壮族自治区土壤肥料工作站 | The implantation methods of selenium-rich peanuts |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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