CN106386313A - Selenium-enriched rice planting method - Google Patents
Selenium-enriched rice planting method Download PDFInfo
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- CN106386313A CN106386313A CN201610869592.XA CN201610869592A CN106386313A CN 106386313 A CN106386313 A CN 106386313A CN 201610869592 A CN201610869592 A CN 201610869592A CN 106386313 A CN106386313 A CN 106386313A
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- Prior art keywords
- selenium
- rice
- fermentation
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- planting method
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
- A01C21/005—Following a specific plan, e.g. pattern
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/20—Cereals
- A01G22/22—Rice
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
- C05D9/02—Other inorganic fertilisers containing trace elements
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The invention discloses a selenium-enriched rice planting method. The method includes the following steps that a nutrient solution is sprayed in the later tillering stage of rice until the leaf surface is uniformly covered with the nutrient solution, and the nutrient solution is prepared from the following preparing method including the steps that 1, raw materials are prepared, wherein 100 parts of purified water, 1.5-2.5 parts of L-selenomethionine and 0.5-1.5 parts of hydroxyethyl cellulose are prepared; 2, after being mixed, the raw materials in the step 1 are put into a steam bursting tank at the pressure of 4MPa, the materials are heated to 150 DEG C and kept at the temperature for 2 min, then pressure is fast released within 1-3 s to obtain slurry, the slurry is inoculated with bacillus subtilis liquid strain cultured to be mature according to the volume ratio of 5% at the temperature of 25 DEG C, the mixture is put into a fermentation tank, sterile air is introduced into the fermentation tank, stirring is carried out for fermentation at the stirring speed of 250 r/min, the fermentation time is 3 h, the temperature is kept at 35-37 DEG C, and after fermentation is finished, the temperature is rapidly raised to 70 DEG C and kept for 0.5 h to carry out inactivation to obtain the nutrient solution.
Description
Technical field
The present invention relates to Rice Cropping technical field, more particularly, to rice rich in selenium paddy rice planting method.
Background technology
Oryza sativa L., originates in China, Oryza sativa L. institute knot reality is Oryza glutinosa, claims rice or rice, be world's staple food crop after shelling
One of.Before 7000 years, Yangtze River in China basin is with regard to rice cultivation.Rice in China sowing face accounts for the 1/4 of national cereal crops, and yield
Then account for more than half.According to different methods, Oryza sativa L. can be divided into long-grained nonglutinous rice and japonica rice, early rice and middle late rice, Oryza sativa L. var. glutinosa Matsum. and non-Oryza sativa L. var. glutinosa Matsum..
Oryza sativa L. institute knot reality is Oryza glutinosa, and Oryza glutinosa (grain) claims rice, scented rice, rice after shelling.Nearly half population in the world, with rice be all
Food.The existing 14000-18000 of cultivation history.For important cereal crops, can starch processed, wine brewing, vinegar processed, Testa oryzae can refine sugar, squeeze
Oil, extraction furfural, for industrial and pharmaceutical；Rice straw is good feedstuff and paper making raw material and braided material, and Fructus Setariae Germinatuss and rice root are available for
Medicinal.The eating method of rice is varied, has rice, congee, rice cake, rice cake, rice wine etc..Oryza sativa L., in addition to edible, also may be used
Make the raw material of industry to make wine, to refine sugar, rice husk, rice straw, can be used as feedstuff.China Oryza sativa L. main producing region is mainly the Northeast, length
River basin, Pearl River Delta.Belong to direct economy crop.Still the staple food of 1/3rd mankind in the world.
Selenium is trace element needed by human.Selenium participates in multiple selenoenzymes and Selenonic protein in synthesized human.Wherein paddy Guang
Sweet peptide peroxidase, is catalyzed hydroperoxides in vivo or lipid peroxide is changed into water or various alcohols, eliminates
Free radical, to biomembranous attack, protects biomembrane from oxidative damage；Selenium participates in constituting the de- iodine enzyme of iodate thyroid amino acid.?
Internal selenium and Vitamin E are worked in coordination with, and can protect cell membrane, prevent the oxidation of unsaturated fatty acid.Trace selenium has protective effect on cancer risk
And liver-protective effect.Selenium can improve human immunity, promotes the propagation of lymphocyte and the synthesis of antibody and immunoglobulin.
Selenium has the obvious effect suppressing and protecting to kinds cancers such as colon cancer, skin carcinoma, hepatocarcinoma, breast carcinoma, and it is in body
Mesostate methyl enol there is stronger active anticancer.Selenium is sought with Vitamin E, garlicin, linoleic acid, germanium, zinc etc.
Foster element has collaborative antioxidative effect, increases antioxidant activity.Meanwhile, selenium has the work mitigating and alleviating heavy metal toxicity
With.
Scientific circles' research finds, the height of blood selenium level is closely bound up with the generation of cancer.Substantial amounts of survey data explanation, one
Individual area food and the height of Selenium in Soils content have direct relation with the sickness rate of cancer, for example：This regional food and soil
Se content in earth is high, and the M ＆ M of cancer is just low, conversely, this regional cancer morbidity and mortality rate are just
Height, the generation close relation of true explanation selenium and cancer.Simultaneously scientific circles also identify selenium have prophylaxis of cancer effect, be
" king of anti-cancer " of body trace element.Because inorganic selenium salt toxicity is larger, forbid in food and feedstuff within 1993 in Japan
Middle interpolation, supports using organic form selenium such as the higher Selenonic protein of safety, aminoacid, or Se-rich farm products.
The research to Selenium rich rice, rice rich in selenium at present, such as Chinese patent CN1608435A, a kind of Selenium rich rice
Cultural method, discloses a kind of selenium-rich agent being made up of foliage-spray cultivation selenium-rich water inorganic compound containing selenium and phosphate
The method of rice；CN101228834A, a kind of production method of nutritious rice rich in Se, disclose a kind of in paddy growth not
Same time, spray the mixed liquor being mixed by RE microbial fertilizer, sodium selenite and boric acid solution, to produce selenium-rich nutritional rice
Method；CN1844062A is a kind of to spray selenium-enriched fertilizer and preparation method thereof in leaf, disclose a kind of containing selenium and with not on year-on-year basis
Foliage fertilizer formulation of composition such as trace element and preparation method thereof in the nitrogen of example, phosphorus, potassium and the multiple beneficial such as zinc, magnesium, copper.
Above-mentioned prior art is all the selenium source using inorganic selenium (selenate or selenite) as fertilizer, and inorganic selenium remain
Amount is big, and inorganic selenium have obvious toxic action to body, and safety is relatively low；Using the method for foliage-spray inorganic selenium, it is excellent
Point is that Crop is fast, but its shortcoming is it is also obvious that foliage-spray first requires selenium fertilizer comparision contents high, such as
CN1382374A, the selenic acid in plant rich in selenium nutrition regulating agent or the content of the salt of Monohydrated selenium dioxide are 4-25%, and the selenium of high concentration is fertile
Increased with fertile cost.
Content of the invention
It is an object of the invention to provide rice rich in selenium paddy rice planting method, by rice tillering this nutrition of latter stage foliage-spray
Liquid, reach realize rice high yield increase income, the effect of selenium element stable content.
Rice rich in selenium paddy rice planting method of the present invention, on the basis of existing technology, method is as follows：
In rice tillering latter stage spray nutritious liquor, so that blade face uniform fold to be defined, described nutritional solution, by following preparation
Method obtains：
1) raw material prepares：Prepare 100 parts of pure water, L- Selenomethionine 1.5-2.5 part, hydroxyethyl cellulose 0.5-1.0 part；
2) by step 1) raw material mixing after, put into steam explosion tank, pressure 4MPa, be heated to 150 DEG C, keep 2min,
Then in 1-3s, quick release pressure obtains serosity, presses percent by volume at 25 DEG C and accesses the ripe hay of culture for 5-10%
Bacillus cereuss liquid strain, puts in fermentation tank, is passed through filtrated air in fermentation tank, is stirred fermenting, after fermentation ends, fast
Temperature is risen to 70 DEG C and keeps 0.5-1.0h inactivation by speed, obtains.
Spray nutritious liquor of the present invention, sprays 1-2 time after preferably nutritional solution being diluted 3-5 times.
Step 1 of the present invention) described in raw material, preferably 100 parts of pure water, 2.0 parts of L- Selenomethionine, hydroxyethyl cellulose
0.8 part, described L- Selenomethionine, i.e. L- selenomethionine, it is also called Selenomethionine.
Step 2) described in the preferred 250r/min of speed of agitator, described fermentation, preferable temperature keep 35-37 DEG C, during fermentation
Between be 3-4h.
The cultural method of described bacillus subtilises liquid spawn is：Using triangular flask strain cultivation, below attach most importance to
Amount percent, by the albumen processing slurry 30.0-35.0%, KH2P041.0%, MgS042.0%, NaCl0.5% put into
In triangular flask, stir, adjust pH to 5.6,125 DEG C of sterilizing 10min, be cooled to 25 DEG C, access withered through slant culture
Careless Bacillus, 28 DEG C of shaking table cultures 60-100h, for fermentation tank inoculation.
Compared to existing technology, it is an advantage of the current invention that：
1st, Selenium-rich nutrient solution of the prior art, usually using inorganic selenium, particularly sodium selenite and sodium selenate, sub- selenium
Sour potassium and potassium selenate etc., obtain from the side-product of metal mineral reserves, and inorganic selenium have larger toxicity, and are difficult to be absorbed, as
Nutritional solution, assimilation effect is bad.The Selenomethionine that the present invention uses, is a kind of organic selenium source, compared with inorganic selenium, seleno egg ammonia
Acid has safe and efficient advantage, and it can substitute some methionine and be attached to the reversible selenium storage of formation one on body protein
Storehouse, and there is not toxic hazard.
2nd, Selenomethionine is that in nature, selenium is present in one of plant and feed grain seleno amino with organic form
The plant growings such as acid, frumentum and forage crop are not necessarily required to Selenomethionine, but with Methionine synthesis they can utilize ring
Selenite particularly in soil and selenate synthesis Selenomethionine in border.Available selenium in soil is more, in plant
Selenomethionine content is higher.At present, Selenomethionine is mainly derived from natural selenium yeast, is being primarily present of organic selenium in selenium yeast
Form, but Selenomethionine has dense yeast taste, directly water-soluble rear sprinkling, and absorption efficiency is low and influences whether finally to produce
The taste and flavor of product.Bacillus subtillis, are one kind of Bacilluss, and bacillus subtilises thalline itself synthesizes alphalise starch
The enzymes such as enzyme, protease, Digestive Enzyme, cellulase, are equivalent to compound enzyme, and the present invention is by Selenomethionine mixing Ko subtilis bar
Bacterium after fermentation, is fermented using the zymasises that Selenomethionine product carries, and is on the one hand beneficial to selenium element and enters Oryza sativa L., on the other hand
With Methionine synthesis they can be using the selenite particularly in soil and selenate synthesis Selenomethionine in environment, also
Can be effectively improved, dissolve yeast flavor.
3rd, steam explosion be using high-temperature high-pressure steam process biomass material, and realized by moment pressure leak process right
The surface texture change of raw material, Component seperation and hemicellulose degradation, are generally used for corn straw, straw and Wooden Biomass etc.
The pretreatment of raw material.The present invention passes through " to put into steam explosion tank, pressure 4MPa, be heated to 150 DEG C, keep 2min, then in 1-
Quick release pressure in 3s ", on the one hand can change to sterilize by the moment of pressure, on the other hand can effectively refine, divide
Scattered Selenomethionine, advantageously in the absorption of Oryza sativa L..
4th, the present invention with the addition of hydroxyethyl cellulose in nutritional solution, has good thickening, suspension, dispersion, emulsifying, glues
The characteristics such as conjunction, film forming, protection moisture and offer protecting colloid, using so that Selenomethionine fully sticks in nutritional solution
On blade face, branch, beneficial to the absorption to nutritional labeling for the blade face, the rice being obtained using the technology of the present invention, according to GB/T 22499-
2008《The standard test of selenium-rich Oryza glutinosa》In rice, the content of selenium is 0.27-0.38mg/kg.
Specific embodiment
With embodiment, the invention will be further described below, but the invention is not limited in these embodiments.
Embodiment 1：
Rice rich in selenium paddy rice planting method, method is as follows：
Spray 1 time in rice tillering latter stage after nutritional solution is diluted 3 times, so that blade face uniform fold to be defined, described nutrition
Liquid, is prepared by the following preparation method：
1) raw material prepares：Prepare 100 parts of pure water, 1.5 parts of L- Selenomethionine, 1.0 parts of hydroxyethyl cellulose；
2) by step 1) raw material mixing after, put into steam explosion tank, pressure 4MPa, be heated to 150 DEG C, keep 2min,
Then in 1-3s, quick release pressure obtains serosity, at 25 DEG C by the hay bud that percent by volume is that maturation is cultivated in 5% access
Spore bacillus liquid spawn, puts in fermentation tank, is passed through filtrated air in fermentation tank, is stirred fermenting, speed of agitator 250r/
Min, fermentation time is 3h, and temperature keeps 35-37 DEG C, after fermentation ends, rapidly temperature is risen to 70 DEG C and keeps 0.5h inactivation, obtain
Arrive.
Embodiment 2：
Rice rich in selenium paddy rice planting method, method is as follows：
Spray 1 time in rice tillering latter stage after nutritional solution is diluted 5 times, so that blade face uniform fold to be defined, described nutrition
Liquid, is prepared by the following preparation method：
1) raw material prepares：Prepare 100 parts of pure water, 2.5 parts of L- Selenomethionine, 0.5 part of hydroxyethyl cellulose；
2) by step 1) raw material mixing after, put into steam explosion tank, pressure 4MPa, be heated to 150 DEG C, keep 2min,
Then in 1-3s, quick release pressure obtains serosity, at 25 DEG C by the hay bud that percent by volume is that maturation is cultivated in 5% access
Spore bacillus liquid spawn, puts in fermentation tank, is passed through filtrated air in fermentation tank, is stirred fermenting, speed of agitator 250r/
Min, fermentation time is 3h, and temperature keeps 35-37 DEG C, after fermentation ends, rapidly temperature is risen to 70 DEG C and keeps 0.5h inactivation, obtain
Arrive.
Embodiment 3：
Rice rich in selenium paddy rice planting method, method is as follows：
Spray 2 times in rice tillering latter stage after nutritional solution is diluted 4 times, so that blade face uniform fold to be defined, described nutrition
Liquid, is prepared by the following preparation method：
1) raw material prepares：Prepare 100 parts of pure water, 2.0 parts of L- Selenomethionine, 0.8 part of hydroxyethyl cellulose；
2) by step 1) raw material mixing after, put into steam explosion tank, pressure 4MPa, be heated to 150 DEG C, keep 2min,
Then in 1-3s, quick release pressure obtains serosity, at 25 DEG C by the hay bud that percent by volume is that maturation is cultivated in 5% access
Spore bacillus liquid spawn, puts in fermentation tank, is passed through filtrated air in fermentation tank, is stirred fermenting, speed of agitator 250r/
Min, fermentation time is 3h, and temperature keeps 35-37 DEG C, after fermentation ends, rapidly temperature is risen to 70 DEG C and keeps 0.5h inactivation, obtain
Arrive.
Comparative example：
Spray 2 times in rice tillering latter stage after nutritional solution is diluted 4 times, so that blade face uniform fold to be defined, described nutrition
Liquid, is prepared by the following preparation method：
1) raw material prepares：Prepare 100 parts of pure water, 2.0 parts of L- Selenomethionine, 0.8 part of hydroxyethyl cellulose；
2) by step 1) raw material mixing after obtain.
Experimental example：
The three batch rice being obtained using the embodiment of the present invention 3, the selenium element content of rice is 0.27mg/ respectively after testing
kg、0.30mg/kg、0.38mg/kg；
The three batch rice being obtained using comparative example, after testing the selenium element content of rice be respectively 0.08mg/kg,
0.07mg/kg、0.06mg/kg.
Claims (5)
1. rice rich in selenium paddy rice planting method is it is characterised in that method is as follows：
In rice tillering latter stage spray nutritious liquor, so that blade face uniform fold to be defined, described nutritional solution, by following preparation method
Obtain：
1) raw material prepares：Prepare 100 parts of pure water, L- Selenomethionine 1.5-2.5 part, hydroxyethyl cellulose 0.5-1.0 part；
2) by step 1) raw material mixing after, put into steam explosion tank, pressure 4MPa, be heated to 150 DEG C, keep 2min, then
In 1-3s, quick release pressure obtains serosity, at 25 DEG C by the bacillus subtilis that percent by volume is that maturation is cultivated in 5% access
Bacteria liquid strain, puts in fermentation tank, is passed through filtrated air in fermentation tank, is stirred fermenting, and speed of agitator 250r/min sends out
The ferment time is 3h, and temperature keeps 35-37 DEG C, after fermentation ends, rapidly temperature is risen to 70 DEG C and keeps 0.5h inactivation, obtain.
2. rice rich in selenium paddy rice planting method according to claim 1 it is characterised in that：Described spray nutritious liquor, is to seek
Nutrient solution sprays 1-2 time after diluting 3-5 times.
3. rice rich in selenium paddy rice planting method according to claim 1 it is characterised in that：Step 1) described in raw material, be pure
100 parts of water purification, 2.0 parts of L- Selenomethionine, 0.8 part of hydroxyethyl cellulose.
4. rice rich in selenium paddy rice planting method according to claim 1 it is characterised in that：Step 2) described in speed of agitator
250r/min, described fermentation, temperature keeps 35-37 DEG C, and fermentation time is 3-4h.
5. rice rich in selenium paddy rice planting method according to claim 1 it is characterised in that：Step 2) described bacillus subtilises
The cultural method of liquid spawn is：Using triangular flask strain cultivation, below for percetage by weight, by the egg albumen powder processing
Slurry 30.0-35.0%, KH2PO41.0%, MgSO42.0%, NaCl0.5% put in triangular flask, stir, adjust pH to
5.6,125 DEG C of sterilizing 10min, are cooled to 25 DEG C, access the Bacillus subtilis strain through slant culture, 28 DEG C of shaking table cultures
60-100h, for fermentation tank inoculation.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107691143A (en) * | 2017-11-02 | 2018-02-16 | 桂林浩新科技服务有限公司 | The implantation methods of selenium-rich corn |
| CN108713382A (en) * | 2018-05-03 | 2018-10-30 | 梁秀芳 | The cultural method of selenium-rich beautiful millettia root |
| CN111533602A (en) * | 2020-04-24 | 2020-08-14 | 安徽汉合生物科技有限公司 | Special foliar fertilizer for selenium-rich biological rice |
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| CN102498995A (en) * | 2011-10-25 | 2012-06-20 | 惠州市海纳粮油食品有限公司 | Production method for organic selenium-rich iron-rich nutrition enriched rice |
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| CN111533602A (en) * | 2020-04-24 | 2020-08-14 | 安徽汉合生物科技有限公司 | Special foliar fertilizer for selenium-rich biological rice |
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