CN106278666A - Rice rich in selenium Rice Cropping nutritional solution - Google Patents
Rice rich in selenium Rice Cropping nutritional solution Download PDFInfo
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- CN106278666A CN106278666A CN201610869990.1A CN201610869990A CN106278666A CN 106278666 A CN106278666 A CN 106278666A CN 201610869990 A CN201610869990 A CN 201610869990A CN 106278666 A CN106278666 A CN 106278666A
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- rice
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
- C05D9/02—Other inorganic fertilisers containing trace elements
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Soil Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses rice rich in selenium Rice Cropping nutritional solution, be prepared by the following preparation method: 1) raw material preparation: prepare pure water 100 parts, L Selenomethionine 1.5 2.5 parts, hydroxyethyl cellulose 0.5 1.0 parts;2) by step 1) raw material mixing after, put in pressure pan, be forced into 4kg/cm2Make its pressure uniform, and keep 2 3min, in 1 3s, then recover normal pressure, obtain serosity, 25 DEG C by volume percentage ratio be 5 10% to access and cultivate ripe bacillus subtilis liquid spawns, put in fermentation tank, fermentation tank be passed through filtrated air, it is stirred fermentation, after fermentation ends, rapidly temperature is risen to 70 DEG C and keeps 0.5 1.0h inactivation, obtain.
Description
Technical field
The present invention relates to Rice Cropping technical field, particularly relate to rice rich in selenium Rice Cropping nutritional solution.
Background technology
Oryza sativa L., originates in China, the Oryza sativa L. institute i.e. Oryza glutinosa of knot reality, claims rice or rice, be world's staple food crop after shelling
One of.Before 7000 years, Yangtze River in China basin is with regard to rice cultivation.Rice in China sowing face accounts for the 1/4 of whole nation cereal crops, and yield
Then account for more than half.According to different methods, Oryza sativa L. can be divided into long-grained nonglutinous rice and japonica rice, early rice and middle late rice, Oryza sativa L. var. glutinosa Matsum. and non-Oryza sativa L. var. glutinosa Matsum..
The Oryza sativa L. institute i.e. Oryza glutinosa of knot reality, Oryza glutinosa (grain) claims rice, scented rice, rice after shelling.Nearly half population in the world, with rice be all
Food.The existing 14000-18000 of cultivation history.For important cereal crops, can starch processed, make wine, vinegar processed, Testa oryzae can refine sugar, squeeze
Oil, extraction furfural, for industrial and pharmaceutical;Rice straw is good feedstuff and paper making raw material and braided material, Fructus Setariae Germinatus and rice root are available for
Medicinal.The eating method of rice is varied, has rice, congee, rice cake, rice cake, rice wine etc..Oryza sativa L., in addition to edible, also may be used
With wine brewing, refine sugar and make the raw material of industry, rice husk, rice straw, can be as feedstuff.China Oryza sativa L. main producing region is mainly the Northeast, length
Basin, river, Pearl River Delta.Belong to direct economy crop.The staple food of 1/3rd mankind the most in the world.
Selenium is the trace element of needed by human.Selenium participates in multiple selenoenzyme and Selenonic protein in synthesized human.Its two-story valley Guang
Sweet peptide peroxidase, catalysis hydroperoxides or lipid peroxide are changed into water or various alcohols in vivo, eliminate
The biomembranous attack of radical pair, protection biomembrane is from oxidative damage;Selenium participates in constituting iodate thyroid amino acid and takes off iodine enzyme.?
Internal selenium and vitamin E are worked in coordination with, it is possible to protection cell membrane, prevent the oxidation of unsaturated fatty acid.Trace selenium has protective effect on cancer risk
And liver-protective effect.Selenium can improve human immunity, the propagation of promotion lymphocyte and antibody and the synthesis of immunoglobulin.
Selenium has significantly suppression and the effect of protection to kinds cancers such as colon cancer, skin carcinoma, hepatocarcinoma, breast carcinoma, and it is in body
Mesostate methyl enol there is stronger active anticancer.Selenium is sought with vitamin E, garlicin, linoleic acid, germanium, zinc etc.
Foster element has collaborative antioxidative effect, increases antioxidant activity.Meanwhile, selenium has the work alleviating and alleviating heavy metal toxicity
With.
Scientific circles' research finds, the height of blood selenium level is closely bound up with the generation of cancer.Substantial amounts of survey data illustrates, one
Individual area food and the height of Selenium in Soils content have direct relation with the sickness rate of cancer, such as: the food in this area and soil
Se content in earth is high, and the M & M of cancer is the lowest, otherwise, the cancer morbidity in this area and mortality rate are just
Height, true explanation selenium and the generation close relation of cancer.Scientific circles also identify selenium and have prophylaxis of cancer effect simultaneously, are
" king of anti-cancer " of body trace element.Owing to inorganic selenium salt toxicity is relatively big, within 1993, forbid at food and feedstuff in Japan
Middle interpolation, supports to use the organic form selenium such as the higher Selenonic protein of safety, aminoacid, or Se-rich farm products.
At present to Selenium rich rice, the research of rice rich in selenium, such as Chinese patent CN1608435A, a kind of Selenium rich rice
Cultural method, discloses and a kind of is cultivated selenium-rich water by foliage-spray by the selenium-rich agent that forms containing selenium inorganic compound and phosphate
The method of rice;CN101228834A, the production method of a kind of nutritious rice rich in Se, disclose a kind of at paddy growth not
Same time, spray the mixed liquor being mixed by RE microbial fertilizer, sodium selenite and boric acid solution, to produce selenium-rich nutritional rice
Method;CN1844062A mono-kind sprays the selenium-enriched fertilizer and preparation method thereof in leaf, disclose a kind of containing selenium and with the most on year-on-year basis
Foliage fertilizer formulation of the composition such as trace element and preparation method thereof in the multiple beneficial such as the nitrogen of example, phosphorus, potassium and zinc, magnesium, copper.
Above-mentioned prior art is all the selenium source using inorganic selenium (selenate or selenite) as fertilizer, and inorganic selenium remain
Amount is big, and inorganic selenium have obvious toxic action to body, and safety is relatively low;The method using foliage-spray inorganic selenium, it is excellent
Point is that Crop is fast, but its shortcoming is it is also obvious that first foliage-spray requires that selenium fertilizer comparision contents is high, as
CN1382374A, selenic acid or the content of the salt of Monohydrated selenium dioxide in plant rich in selenium nutrition regulating agent are 4-25%, and the selenium of high concentration is fertile
Add with fertile cost.
Summary of the invention
It is an object of the invention to provide rice rich in selenium Rice Cropping nutritional solution, by this battalion of rice tillering foliage-spray in latter stage
Nutrient solution, reaches to realize the effect that rice high yield increases income, selenium constituent content is stable.
Rice rich in selenium Rice Cropping nutritional solution of the present invention, is prepared by the following preparation method:
1) raw material prepares: prepare pure water 100 parts, L-Selenomethionine 1.5-2.5 part, hydroxyethyl cellulose 0.5-1.0 part;
2) by step 1) raw material mixing after, put in pressure pan, be forced into 4kg/cm2So that it is pressure is uniform, and keeps
2-3min, then recovers normal pressure in 1-3s, obtains serosity, 25 DEG C by volume percentage ratio be 5-10% access cultivate ripe
Bacillus subtilis liquid spawn, puts in fermentation tank, is passed through filtrated air in fermentation tank, is stirred fermentation, fermentation ends
After, rapidly temperature is risen to 70 DEG C and keeps 0.5-1.0h inactivation, obtain.
Step 1 of the present invention) described in raw material, preferably pure water 100 parts, L-Selenomethionine 2.0 parts, hydroxyethyl cellulose
0.8 part, described L-Selenomethionine, i.e. L-selenomethionine, it is also called Selenomethionine.
Step 2) described in the preferred 250r/min of speed of agitator, described fermentation, preferable temperature keep 35-37 DEG C, during fermentation
Between be 3-4h.
The cultural method of described bacillus subtilis liquid spawn is: uses triangular flask strain cultivation, below attaches most importance to
Amount percent, albumen slurry 30.0-35.0% that will process, KH2P041.0%, MgS042.0%, NaCl0.5% put into
In triangular flask, stir, regulate pH to 5.6,125 DEG C of sterilizing 10min, be cooled to 25 DEG C, access withered through slant culture
Grass Bacillus, 28 DEG C of shaking tables are cultivated 60-100h, are inoculated for fermentation tank.
Nutritional solution of the present invention is used for rice rich in selenium Rice Cropping, sprays this nutritional solution 1-2 time latter stage at rice tillering, preferably will
Nutritional solution sprays, to be as the criterion by blade face uniform fold after diluting 3-5 times.
Compared to existing technology, it is an advantage of the current invention that:
1, Selenium-rich nutrient solution of the prior art, usually uses inorganic selenium, particularly sodium selenite and sodium selenate, sub-selenium
Acid potassium and potassium selenate etc., obtain from the side-product of metal mineral reserves, and inorganic selenium have bigger toxicity, and are difficult to be absorbed, as
Nutritional solution, assimilation effect is bad.The Selenomethionine that the present invention uses, is a kind of organic selenium source, compared with inorganic selenium, and seleno egg ammonia
Acid has safe and efficient advantage, and it can be attached on body protein form a reversible selenium storage by Substitute For Partial methionine
Storehouse, and there is not toxic hazard.
2, a kind of seleno amino that during Selenomethionine is nature, selenium is present in plant and feed grain with organic form
Acid, the plant growing such as frumentum and forage crop is not necessarily required to Selenomethionine, but along with methionine synthesis they can utilize ring
The particularly selenite in soil and selenate synthesis Selenomethionine in border.Available selenium in soil is the most, in plant
Selenomethionine content is the highest.At present, Selenomethionine is mainly derived from natural selenium yeast, is being primarily present of organic selenium in selenium yeast
Form, but Selenomethionine has dense yeast taste, and direct water-soluble rear sprinkling, absorption efficiency is low and influences whether finally to produce
The taste and flavor of product.Bacillus subtillis, is the one of Bacillus, bacillus subtilis thalline self synthesis alphalise starch
The enzymes such as enzyme, protease, lipase, cellulase, are equivalent to compound enzyme, and the present invention passes through Selenomethionine mixing Ko subtilis bar
Bacterium after fermentation, utilize Selenomethionine product with zymasis fermentation, on the one hand be beneficial to selenium element and enter Oryza sativa L., on the other hand
Along with methionine synthesis they can utilize in environment the selenite in particularly soil and selenate synthesis Selenomethionine, also
Can be effectively improved, dissolve yeast flavor.
3, the HIGH PRESSURE TREATMENT that the food sterilization stage uses is applied to the compounding stage by the present invention, by " being forced into
4kg/cm2So that it is pressure is uniform, and keeps 2-3min, then recovers normal pressure in 1-3s ", by moment, pressure is reduced to 0,
On the one hand can be changed by the moment of pressure and carry out sterilizing, on the other hand can effectively refine, disperse Selenomethionine, advantageously
Absorption in Oryza sativa L..
4, the present invention with the addition of hydroxyethyl cellulose in nutritional solution, have good thickening, suspend, disperse, emulsifying, viscous
The characteristics such as conjunction, film forming, protection moisture and offer protecting colloid, use so that Selenomethionine fully sticks in nutritional solution
On blade face, branch, the beneficially blade face absorption to nutritional labeling, use the rice that the technology of the present invention obtains, according to GB/T 22499-
In 2008 " standard test of selenium-rich Oryza glutinosa " rice, the content of selenium is 0.27-0.38mg/kg.
Detailed description of the invention
With embodiment, the invention will be further described below, but the invention is not limited in these embodiments.
Embodiment 1:
Rice rich in selenium Rice Cropping nutritional solution, is prepared by the following preparation method:
1) raw material prepares: prepare pure water 100 parts, L-Selenomethionine 1.5 parts, hydroxyethyl cellulose 1.0 parts;
2) by step 1) raw material mixing after, put in pressure pan, be forced into 4kg/cm2So that it is pressure is uniform, and keeps
2-3min, then recovers normal pressure in 1-3s, obtains serosity, 25 DEG C by volume percentage ratio to be that 5% access is cultivated ripe withered
Grass bacillus cereus liquid strain, puts in fermentation tank, is passed through filtrated air in fermentation tank, is stirred fermentation, speed of agitator
250r/min, fermentation time is 3h, and temperature keeps 35-37 DEG C, after fermentation ends, temperature rises to rapidly 70 DEG C and keeps 0.5h to go out
Live, obtain.
Embodiment 2:
Rice rich in selenium Rice Cropping nutritional solution, is prepared by the following preparation method:
1) raw material prepares: prepare pure water 100 parts, L-Selenomethionine 2.5 parts, hydroxyethyl cellulose 0.5 part;
2) by step 1) raw material mixing after, put in pressure pan, be forced into 4kg/cm2So that it is pressure is uniform, and keeps
2-3min, then recovers normal pressure in 1-3s, obtains serosity, 25 DEG C by volume percentage ratio to be that 10% access is cultivated ripe withered
Grass bacillus cereus liquid strain, puts in fermentation tank, is passed through filtrated air in fermentation tank, is stirred fermentation, speed of agitator
250r/min, fermentation time is 4h, and temperature keeps 35-37 DEG C, after fermentation ends, temperature rises to rapidly 70 DEG C and keeps 1.0h to go out
Live, obtain.
Embodiment 3:
Rice rich in selenium Rice Cropping nutritional solution, is prepared by the following preparation method:
1) raw material prepares: prepare pure water 100 parts, L-Selenomethionine 2.0 parts, hydroxyethyl cellulose 0.8 part;
2) by step 1) raw material mixing after, put in pressure pan, be forced into 4kg/cm2So that it is pressure is uniform, and keeps
2-3min, then recovers normal pressure in 1-3s, obtains serosity, 25 DEG C by volume percentage ratio to be that 8% access is cultivated ripe withered
Grass bacillus cereus liquid strain, puts in fermentation tank, is passed through filtrated air in fermentation tank, is stirred fermentation, speed of agitator
250r/min, fermentation time is 3h, and temperature keeps 35-37 DEG C, after fermentation ends, temperature rises to rapidly 70 DEG C and keeps 0.5h to go out
Live, obtain.
Comparative example:
Rice rich in selenium Rice Cropping nutritional solution, is prepared by the following preparation method:
1) raw material prepares: prepare pure water 100 parts, L-Selenomethionine 2.0 parts, hydroxyethyl cellulose 0.8 part;
2) by step 1) raw material mixing after obtain.
Experimental example:
Using the three batch rice that the embodiment of the present invention 3 obtains, the selenium constituent content of rice is 0.27mg/ respectively after testing
kg、0.30mg/kg、0.38mg/kg;
Use the three batch rice that obtain of comparative example, after testing the selenium constituent content of rice be respectively 0.08mg/kg,
0.07mg/kg、0.06mg/kg。
Claims (4)
1. rice rich in selenium Rice Cropping nutritional solution, it is characterised in that be prepared by the following preparation method:
1) raw material prepares: prepare pure water 100 parts, L-Selenomethionine 1.5-2.5 part, hydroxyethyl cellulose 0.5-1.0 part;
2) by step 1) raw material mixing after, put in pressure pan, be forced into 4kg/cm2So that it is pressure is uniform, and keeps 2-
3min, then recovers normal pressure in 1-3s, obtains serosity, 25 DEG C by volume percentage ratio be 5-10% access cultivate ripe withered
Grass bacillus cereus liquid strain, puts in fermentation tank, is passed through filtrated air in fermentation tank, is stirred fermentation, after fermentation ends,
Rapidly temperature is risen to 70 DEG C and keeps 0.5-1.0h inactivation, obtain.
Rice rich in selenium Rice Cropping nutritional solution the most according to claim 1, it is characterised in that: step 1) described in raw material, for
Pure water 100 parts, L-Selenomethionine 2.0 parts, hydroxyethyl cellulose 0.8 part.
Rice rich in selenium Rice Cropping nutritional solution the most according to claim 1, it is characterised in that: step 2) described in speed of agitator
250r/min, described fermentation, temperature keeps 35-37 DEG C, and fermentation time is 3-4h.
Rice rich in selenium Rice Cropping nutritional solution the most according to claim 1, it is characterised in that: step 2) described bacillus subtilis
The cultural method of bacteria liquid strain is: uses triangular flask strain cultivation, is below percetage by weight, the albumen that will process
Slurry 30.0-35.0%, KH2PO41.0%, MgSO42.0%, NaCl0.5% put in triangular flask, stir, and regulate pH
To 5.6,125 DEG C of sterilizing 10min, it is cooled to 25 DEG C, accesses the Bacillus subtilis strain through slant culture, 28 DEG C of shaking table trainings
Support 60-100h, inoculate for fermentation tank.
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107382519A (en) * | 2017-08-28 | 2017-11-24 | 广西钟山县富强雅瑶华香农业有限公司 | A kind of selenium-rich rice plants nutrient solution |
CN111533602A (en) * | 2020-04-24 | 2020-08-14 | 安徽汉合生物科技有限公司 | Special foliar fertilizer for selenium-rich biological rice |
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CN102415252A (en) * | 2011-08-23 | 2012-04-18 | 南京远望富硒农产品有限责任公司 | Method for preparing selenium-rich rice |
CN102503632A (en) * | 2011-10-25 | 2012-06-20 | 惠州市海纳粮油食品有限公司 | Selenium-rich organic/inorganic fertilizer for producing selenium-rick rice as well as application method thereof |
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CN105272761A (en) * | 2015-11-16 | 2016-01-27 | 南宁穗康农业科技有限公司 | Selenium-enriched biological organic fertilizer and production method thereof |
CN105950503A (en) * | 2016-05-19 | 2016-09-21 | 湖北民族学院 | Bacillus subtilis SE201412 and organic-selenium-rich bactericide |
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CN102391047A (en) * | 2011-08-08 | 2012-03-28 | 江苏浩波农业科技开发有限公司 | Sustained-release soil selenium fertilizer used for degrading residual pesticide and reducing heavy metal content, and preparation method thereof |
CN102415252A (en) * | 2011-08-23 | 2012-04-18 | 南京远望富硒农产品有限责任公司 | Method for preparing selenium-rich rice |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN111533602A (en) * | 2020-04-24 | 2020-08-14 | 安徽汉合生物科技有限公司 | Special foliar fertilizer for selenium-rich biological rice |
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